@misc{TeuschlFuchs, author = {Teuschl, Andreas and Fuchs, Christiane}, title = {Bioreactors in Musculoskeletal Tissue Engineering}, subject = {Bioreactor}, language = {en} } @article{MaleinerTomaschHeheretal., author = {Maleiner, Babette and Tomasch, Janine and Heher, Philipp and Spadiut, Oliver and R{\"u}nzler, Dominik and Fuchs, Christiane}, title = {The Importance of Biophysical and Biochemical Stimuli in Dynamic Skeletal Muscle Models.}, series = {Frontiers in Physiology}, journal = {Frontiers in Physiology}, abstract = {Classical approaches to engineer skeletal muscle tissue based on current regenerative and surgical procedures still do not meet the desired outcome for patient applications. Besides the evident need to create functional skeletal muscle tissue for the repair of volumetric muscle defects, there is also growing demand for platforms to study muscle-related diseases, such as muscular dystrophies or sarcopenia. Currently, numerous studies exist that have employed a variety of biomaterials, cell types and strategies for maturation of skeletal muscle tissue in 2D and 3D environments. However, researchers are just at the beginning of understanding the impact of different culture settings and their biochemical (growth factors and chemical changes) and biophysical cues (mechanical properties) on myogenesis. With this review we intend to emphasize the need for new in vitro skeletal muscle (disease) models to better recapitulate important structural and functional aspects of muscle development. We highlight the importance of choosing appropriate system components, e.g., cell and biomaterial type, structural and mechanical matrix properties or culture format, and how understanding their interplay will enable researchers to create optimized platforms to investigate myogenesis in healthy and diseased tissue. Thus, we aim to deliver guidelines for experimental designs to allow estimation of the potential influence of the selected skeletal muscle tissue engineering setup on the myogenic outcome prior to their implementation. Moreover, we offer a workflow to facilitate identifying and selecting different analytical tools to demonstrate the successful creation of functional skeletal muscle tissue. Ultimately, a refinement of existing strategies will lead to further progression in understanding important aspects of muscle diseases, muscle aging and muscle regeneration to improve quality of life of patients and enable the establishment of new treatment options.}, subject = {Bioreactor}, language = {en} } @article{TomaschMaleinerHeheretal., author = {Tomasch, Janine and Maleiner, Babette and Heher, Philipp and Rufin, Manuel and Andriotis, Orestis G. and Thurner, Philipp J. and Redl, Heinz and Fuchs, Christiane and Teuschl-Woller, Andreas H.}, title = {Changes in Elastic Moduli of Fibrin Hydrogels Within the Myogenic Range Alter Behavior of Murine C2C12 and Human C25 Myoblasts Differently}, series = {Froniers in Bioengineering and Biotechnology}, volume = {10}, journal = {Froniers in Bioengineering and Biotechnology}, pages = {836520}, abstract = {Fibrin hydrogels have proven highly suitable scaffold materials for skeletal muscle tissue engineering in the past. Certain parameters of those types of scaffolds, however, greatly affect cellular mechanobiology and therefore the myogenic outcome. The aim of this study was to identify the influence of apparent elastic properties of fibrin scaffolds in 2D and 3D on myoblasts and evaluate if those effects differ between murine and human cells. Therefore, myoblasts were cultured on fibrin-coated multiwell plates ("2D") or embedded in fibrin hydrogels ("3D") with different elastic moduli. Firstly, we established an almost linear correlation between hydrogels' fibrinogen concentrations and apparent elastic moduli in the range of 7.5 mg/ml to 30 mg/ml fibrinogen (corresponds to a range of 7.7-30.9 kPa). The effects of fibrin hydrogel elastic modulus on myoblast proliferation changed depending on culture type (2D vs 3D) with an inhibitory effect at higher fibrinogen concentrations in 3D gels and vice versa in 2D. The opposite effect was evident in differentiating myoblasts as shown by gene expression analysis of myogenesis marker genes and altered myotube morphology. Furthermore, culture in a 3D environment slowed down proliferation compared to 2D, with a significantly more pronounced effect on human myoblasts. Differentiation potential was also substantially impaired upon incorporation into 3D gels in human, but not in murine, myoblasts. With this study, we gained further insight in the influence of apparent elastic modulus and culture type on cellular behavior and myogenic outcome of skeletal muscle tissue engineering approaches. Furthermore, the results highlight the need to adapt parameters of 3D culture setups established for murine cells when applied to human cells.}, subject = {Tissue Engineering}, language = {en} } @misc{HeherTomaschMaleineretal., author = {Heher, Philipp and Tomasch, Janine and Maleiner, Babette and Redl, Heinz and Fuchs, Christiane}, title = {The Importance of Biomechanical Cues for In Vitro Skeletal Myogenesis}, subject = {In Vitro}, language = {en} } @article{SchuhHeherWeihsetal., author = {Schuh, Christina and Heher, Philipp and Weihs, Anna and Fuchs, Christiane and Gabriel, Christian and Wolbank, Susanne and Mittermayr, Rainer and Redl, Heinz and R{\"u}nzler, Dominik and Teuschl, Andreas}, title = {In vitro extracorporeal shock wave treatment enhances stemness and preserves multipotency of rat and human adipose-derived stem cells}, series = {Journal of Cytotherapy}, journal = {Journal of Cytotherapy}, subject = {Shockwave}, language = {en} } @misc{SlezakRoseHercheretal., author = {Slezak, Paul and Rose, Roland and Hercher, David and Weihs, Anna and Fuchs, Christiane and Redl, Heinz and Mittermayr, Rainer and Slezak, Cyrill}, title = {Tracking therapeutic shockwaves and their impact on regeneration}, subject = {Shockwave Therapy}, language = {en} } @inproceedings{FuchsWeihsSzwarcetal., author = {Fuchs, Christiane and Weihs, Anna and Szwarc, Dorota and Mittermayr, Rainer and R{\"u}nzler, Dominik and Teuschl, Andreas}, title = {Shock wave treatment of muscle (stem) cells - a new implementation for regeneration}, series = {Proceedings of the 20th International Congress of the ISMST}, booktitle = {Proceedings of the 20th International Congress of the ISMST}, subject = {Shockwave treatment}, language = {en} } @misc{FuchsWeihsSzwarcetal., author = {Fuchs, Christiane and Weihs, Anna and Szwarc, Dorota and Mittermayr, Rainer and R{\"u}nzler, Dominik and Teuschl, Andreas}, title = {Shock wave treatment of muscle (stem) cells - a new implementation for regeneration}, subject = {Shockwave treatment}, language = {en} } @misc{TeuschlWeihsFuchsetal., author = {Teuschl, Andreas and Weihs, Anna and Fuchs, Christiane and Monforte Vila, Xavier}, title = {Silk as a versatile biomaterial for musculoskeletal tissue engineering}, subject = {Silk}, language = {en} } @misc{SzwarcFuchsWeihsetal., author = {Szwarc, Dorota and Fuchs, Christiane and Weihs, Anna and R{\"u}nzler, Dominik}, title = {Molecular mechanisms underlying the potential of shock wave treatment for cardiac therapy}, subject = {Shockwave treatment}, language = {en} } @misc{SzwarcFuchsPurtscheretal., author = {Szwarc, Dorota and Fuchs, Christiane and Purtscher, Michaela and R{\"u}nzler, Dominik}, title = {Elucidating the molecular mechanisms underlying cardiac shock wave therapy}, subject = {Shockwave treatment}, language = {en} } @misc{Fuchs, author = {Fuchs, Christiane}, title = {The importance of mechanotransduction in myogenesis}, subject = {Mechanotransduction}, language = {en} } @inproceedings{MaleinerHeherTeuschletal., author = {Maleiner, Babette and Heher, Philipp and Teuschl, Andreas and Redl, Heinz and R{\"u}nzler, Dominik and Fuchs, Christiane}, title = {Generation of aligned skeletal muscle-like tissue based on the application of strain to a 3D fibrin scaffold}, series = {Proceedings of PACT "Designer Cells go Clinical" Symposium}, booktitle = {Proceedings of PACT "Designer Cells go Clinical" Symposium}, subject = {Tissue Generation}, language = {en} } @misc{SzwarcFuchsWeihsetal., author = {Szwarc, Dorota and Fuchs, Christiane and Weihs, Anna and Monforte Vila, Xavier and Hanetseder, Dominik and Teuschl, Andreas and R{\"u}nzler, Dominik}, title = {The effect of shock waves on in vitro cartilage development in silk scaffolds}, subject = {Shockwave treatment}, language = {en} } @misc{MaleinerHeherTeuschletal., author = {Maleiner, Babette and Heher, Philipp and Teuschl, Andreas and Redl, Heinz and R{\"u}nzler, Dominik and Fuchs, Christiane}, title = {Generation of aligned skeletal muscle-like tissue based on the application of strain to a 3D fibrin scaffold}, subject = {Tissue Generation}, language = {en} } @misc{TeuschlFuchsWeihsetal., author = {Teuschl, Andreas and Fuchs, Christiane and Weihs, Anna and Heimel, Patrick and R{\"u}nzler, Dominik and Redl, Heinz and Nau, Thomas}, title = {The Silk Road from Textiles to Novel Medical Implants}, subject = {Silk}, language = {en} } @inproceedings{FuchsSzwarcWeihsetal., author = {Fuchs, Christiane and Szwarc, Dorota and Weihs, Anna and R{\"u}nzler, Dominik}, title = {Shock wave treatment of 3D cardiac model systems activates ERK 1/2 signaling pathway and influences cardiomyogenesis}, series = {Proceedings of PACT "Designer Cells go Clinical" Symposium}, booktitle = {Proceedings of PACT "Designer Cells go Clinical" Symposium}, subject = {Shockwave treatment}, language = {en} } @inproceedings{FuchsSzwarcWeihsetal., author = {Fuchs, Christiane and Szwarc, Dorota and Weihs, Anna and R{\"u}nzler, Dominik}, title = {Shock wave treatment of 3D cardiac model systems activates ERK 1/2 signaling pathway and influences cardiomyogenesis}, series = {Proceedings of LBG Meeting for Health Sciences 2016}, booktitle = {Proceedings of LBG Meeting for Health Sciences 2016}, subject = {Shockwave treatment}, language = {en} } @misc{FuchsSzwarcWeihsetal., author = {Fuchs, Christiane and Szwarc, Dorota and Weihs, Anna and R{\"u}nzler, Dominik}, title = {Shock wave treatment of 3D cardiac model systems activates ERK 1/2 signaling pathway and influences cardiomyogenesis}, subject = {Shockwave treatment}, language = {en} } @inproceedings{FuchsSzwarcWeihsetal., author = {Fuchs, Christiane and Szwarc, Dorota and Weihs, Anna and R{\"u}nzler, Dominik}, title = {Shock wave treatment positively influences cardiomyogenesis in an energy-dependent manner}, series = {Proceedings of PACT "Designer Cells go Clinical" Symposium}, booktitle = {Proceedings of PACT "Designer Cells go Clinical" Symposium}, subject = {Shockwave treatment}, language = {en} } @inproceedings{BasoliChaudryCrucianietal., author = {Basoli, Valentina and Chaudry, Sidrah and Cruciani, Sara and Fuchs, Christiane and Rieger, Sabine and Dungel, Peter and Wolbank, Susanne and Ventura, Carlo and Grillari-Voglauer, Regina and Redl, Heinz and Maioli, Margherita}, title = {Epigenetic and molecular behavious of stem cells exposed to biophysical stimuli: new insights in regenerative medicine}, series = {Proceedings des Seminars zum 40. Jahresjubil{\"a}um der {\"O}sterreichischen Gesellschaft f{\"u}r Chirurgische Forschung}, booktitle = {Proceedings des Seminars zum 40. Jahresjubil{\"a}um der {\"O}sterreichischen Gesellschaft f{\"u}r Chirurgische Forschung}, subject = {Stem Cells}, language = {en} } @misc{BasoliChaudryCrucianietal., author = {Basoli, Valentina and Chaudry, Sidrah and Cruciani, Gabriele and Fuchs, Christiane and Rieger, Sabine and Dungel, Peter and Wolbank, Susanne and Ventura, Carlo and Grillari-Voglauer, Regina and Redl, Heinz and Maioli, Margherita}, title = {Epigenetic and molecular behavious of stem cells exposed to biophysical stimuli: new insights in regenerative medicine}, subject = {Stem Cells}, language = {en} } @misc{Fuchs, author = {Fuchs, Christiane}, title = {A mechanical stimulation strategy for engineering skeletal muscle-like tissue on a strained fibrin scaffold}, subject = {Muscle Tissue}, language = {en} } @misc{Fuchs, author = {Fuchs, Christiane}, title = {The beneficial effects of in vitro shock wave treatment on cardiomyogenesis are energy dependent}, subject = {In Vitro}, language = {en} } @misc{Fuchs, author = {Fuchs, Christiane}, title = {Mechanotransduction - bioreactors}, subject = {Bioreactors}, language = {en} } @article{HeherMaleinerPruelleretal., author = {Heher, Philipp and Maleiner, Babette and Pr{\"u}ller, Johanna and Teuschl, Andreas and Kollmitzer, Josef and Monforte Vila, Xavier and Wolbank, Susanne and Redl, Heinz and R{\"u}nzler, Dominik and Fuchs, Christiane}, title = {A novel bioreactor for the generation of highly aligned 3D skeletal muscle-like constructs through orientation of fibrin via application of static strain}, series = {Acta Biomaterialia}, journal = {Acta Biomaterialia}, subject = {Bioreactor}, language = {en} } @misc{FuchsWeihsTeuschletal., author = {Fuchs, Christiane and Weihs, Anna and Teuschl, Andreas and Hartinger, Joachim and Slezak, Paul and Mittermayr, Rainer and Redl, Heinz and Junger, Wolfgang and Sitte, Harald and R{\"u}nzler, Dominik}, title = {Shockwave Treatment Enhances Proliferation and Improves Wound Healing via Purinergic Signaling Linked ERK 1/2 Pathways}, subject = {Shockwave treatment}, language = {en} } @misc{FuchsWeihsTeuschletal., author = {Fuchs, Christiane and Weihs, Anna and Teuschl, Andreas and Hartinger, Joachim and Slezak, Paul and Mittermayr, Rainer and Redl, Heinz and Junger, Wolfgang and Sitte, Harald and R{\"u}nzler, Dominik}, title = {Shockwave Treatment Augments Proliferation and Improves Wound Healing via Purinergic Signaling Linked ERK 1/2 Pathways}, subject = {Shockwave treatment}, language = {en} } @misc{HeherFuchsPruelleretal., author = {Heher, Philipp and Fuchs, Christiane and Pr{\"u}ller, Johanna and Babette, Maleiner and R{\"u}nzler, Dominik and Redl, Heinz}, title = {A novel bioreactor for engineering skeletal muscle-like tissue in a strained fibrin scaffold}, subject = {Bioreactor}, language = {en} } @misc{WeihsFuchsTeuschletal., author = {Weihs, Anna and Fuchs, Christiane and Teuschl, Andreas and Hartinger, Joachim and Slezak, Paul and Mittermayr, Rainer and Redl, Heinz and Junger, Wolfgang and Sitte, Harald and R{\"u}nzler, Dominik}, title = {Shockwave treatment activates Erk1/2 pathways predominantly via P2Y receptor involvement}, subject = {Shockwave}, language = {en} } @misc{TeuschlFuchsFeichtingeretal., author = {Teuschl, Andreas and Fuchs, Christiane and Feichtinger, Georg and Heher, Philipp and Heimel, Patrick and Schuh, Christina and N{\"u}rnberger, Sylvia and Nau, Thomas and R{\"u}nzler, Dominik and Redl, Heinz}, title = {Fibrin or Fibroin - Not only the "o" Makes the Difference}, subject = {Fibrin}, language = {en} } @article{WeihsFuchsTeuschletal., author = {Weihs, Anna and Fuchs, Christiane and Teuschl, Andreas and Hartinger, Joachim and Slezak, Paul and Mittermayr, Rainer and Redl, Heinz and Junger, Wolfgang and Sitte, Harald and R{\"u}nzler, Dominik}, title = {Shock Wave Treatment Enhances Cell Proliferation and Improves Wound Healing by ATP Release-coupled Extracellular Signal-regulated Kinase (ERK) Activation}, series = {The Journal of biological chemistry}, journal = {The Journal of biological chemistry}, subject = {Shockwave}, language = {de} } @misc{Fuchs, author = {Fuchs, Christiane}, title = {Shockwave treatment promotes proliferation via purinergic signaling coupled ERK1/2 pathway activation}, subject = {Shockwave}, language = {en} } @misc{HeherFuchsPruelleretal., author = {Heher, Philipp and Fuchs, Christiane and Pr{\"u}ller, Johanna and Maleiner, Babette and Kollmitzer, Josef and R{\"u}nzler, Dominik and Teuschl, Andreas and Wolbank, Susanne and Redl, Heinz}, title = {A bioreactor-based 3D culture system for skeletal muscle engineering in fibrin scaffolds}, subject = {Bioreactors}, language = {en} } @article{ButaDavidDresseletal., author = {Buta, Christiane and David, Robert and Dressel, Ralf and Emgard, Mia and Fuchs, Christiane and Gross, Ulrike and Healy, Lyn and Hescheler, J{\"u}rgen and Kolar, Roman and Martin, Ulrich and Mikkers, Harald and M{\"u}ller, Franz-Josef and Schneider, Rebekka and Seiler, Andrea and Spielmann, Horst and Weitzer, Georg}, title = {REconsidering pluripotency tests: do we still need teratoma assays?}, series = {Stem Cell Research}, volume = {11}, journal = {Stem Cell Research}, number = {1}, pages = {552 -- 562}, subject = {Assay}, language = {en} } @article{RosnerSchipanyGundackeretal., author = {Rosner, Margit and Schipany, Katharina and Gundacker, Claudia and Shanmugasundaram, Parthasaraty and Li, Kongzhao and Fuchs, Christiane and Lubec, Gert and Hengstschl{\"a}ger, Martin}, title = {Renal differentiation of amniotic fluid stem cells: perspectives for clinical application and for studies on specific human genetic diseases}, series = {Eur J Clin Invest}, journal = {Eur J Clin Invest}, subject = {Stem Cells}, language = {en} } @article{GundackerScheinastDamjanovicetal., author = {Gundacker, Claudia and Scheinast, Matthias and Damjanovic, Lukas and Fuchs, Christiane and Rosner, Margit and Hengstschl{\"a}ger, Markus}, title = {Proliferation potential of human amniotic fluid stem cells differently respondes to mercury and lead exposure}, series = {Amino Acids}, journal = {Amino Acids}, subject = {Stem Cells}, language = {en} } @article{RosnerFuchsDolznigetal., author = {Rosner, Margit and Fuchs, Christiane and Dolznig, Helmut and Hengstschl{\"a}ger, Markus}, title = {Different cytoplasmic/nucelar distribution of S6 protein phosphorylated at S240/244 and S235/236}, series = {Amino Acids}, journal = {Amino Acids}, number = {40}, pages = {595 -- 600}, subject = {Proteins}, language = {en} } @misc{MuehlederFuchsBassilioetal., author = {M{\"u}hleder, Severin and Fuchs, Christiane and Bassilio, Jose and Sczwarc, Dorota and Pill, Karoline and Slezak, Paul and Labuda, Krystina and Siehs, Christian and Pr{\"o}ll, Johannes and Priglinger, Eleni and Redl, Heinz and Holnthoner, Wolfgang}, title = {The purinergic receptor P2Y2 modulates endothelial sprouting and angiogenesis}, subject = {Angiogenesis}, language = {en} } @article{PriglingerSchuhSteffenhagenetal., author = {Priglinger, Eleni and Schuh, Christina and Steffenhagen, Carolin and Wurzer, Christoph and Maier, Julia and N{\"u}rnberger, Sylvia and Holnthoner, Wolfgang and Fuchs, Christiane and Suessner, Susanne and R{\"u}nzler, Dominik and Redl, Heinz and Wolbank, Susanne}, title = {Improvement of adipose tissue-derived cells by low-energy extracorporeal shock wave therapy.}, series = {Cytotherapy}, journal = {Cytotherapy}, pages = {1079 -- 1095}, abstract = {BACKGROUND: Cell-based therapies with autologous adipose tissue-derived cells have shown great potential in several clinical studies in the last decades. The majority of these studies have been using the stromal vascular fraction (SVF), a heterogeneous mixture of fibroblasts, lymphocytes, monocytes/macrophages, endothelial cells, endothelial progenitor cells, pericytes and adipose-derived stromal/stem cells (ASC) among others. Although possible clinical applications of autologous adipose tissue-derived cells are manifold, they are limited by insufficient uniformity in cell identity and regenerative potency. METHODS: In our experimental set-up, low-energy extracorporeal shock wave therapy (ESWT) was performed on freshly obtained human adipose tissue and isolated adipose tissue SVF cells aiming to equalize and enhance stem cell properties and functionality. RESULTS: After ESWT on adipose tissue we could achieve higher cellular adenosine triphosphate (ATP) levels compared with ESWT on the isolated SVF as well as the control. ESWT on adipose tissue resulted in a significantly higher expression of single mesenchymal and vascular marker compared with untreated control. Analysis of SVF protein secretome revealed a significant enhancement in insulin-like growth factor (IGF)-1 and placental growth factor (PLGF) after ESWT on adipose tissue. DISCUSSION: Summarizing we could show that ESWT on adipose tissue enhanced the cellular ATP content and modified the expression of single mesenchymal and vascular marker, and thus potentially provides a more regenerative cell population. Because the effectiveness of autologous cell therapy is dependent on the therapeutic potency of the patient's cells, this technology might raise the number of patients eligible for autologous cell transplantation.}, subject = {Shockwave Therapy}, language = {en} } @inproceedings{HoerzerFuchsGastingeretal., author = {H{\"o}rzer, S. and Fuchs, Christiane and Gastinger, R. and Sabo, Anton and Mehnen, Lars and Martinek, Johannes and Reichel, Martin}, title = {Simulation of spinning soccer ball trajectories influenced by altitude}, series = {Procedia Engineering}, booktitle = {Procedia Engineering}, pages = {2461 -- 2466}, subject = {Soccer}, language = {en} } @article{GollmannTepekoeylueGraberHirschetal., author = {Gollmann-Tepek{\"o}yl{\"u}, Can and Graber, Michael and Hirsch, Jakob and Mair, Sophia and Naschberger, Andreas and P{\"o}lzl, Leo and N{\"a}gele, Felix and Kirchmair, Elke and Degenhart, Gerald and Demetz, Egon and Hilbe, Richard and Chen, Hao-Yu and Engert, James C and B{\"o}hm, Anna and Franz, Nadja and Lobenwein, Daniela and Lener, Daniela and Fuchs, Christiane and Weihs, Anna and T{\"o}chterle, Sonja and Vogel, Georg F and Schweiger, Victor and Eder, Jonas and Pietschmann, Peter and Seifert, Markus and Kronenberg, Florian and Coassin, Stefan and Blumer, Michael and Hackl, Hubert and Meyer, Dirk and Feuchtner, Gudrun and Kirchmair, Rudolf and Troppmair, Jakob and Krane, Markus and Weiss, G{\"u}nther and Tsimikas, Sotirios and Thanassoulis, George and Grimm, Michael and Rupp, Bernhard and Huber, Lukas A and Zhang, Shen-Ying and Casanova, Jean-Laurent and Tancevski, Ivan and Holfeld, Johannes}, title = {Toll-Like Receptor 3 Mediates Aortic Stenosis Through a Conserved Mechanism of Calcification}, series = {Circulation}, volume = {147}, journal = {Circulation}, number = {20}, doi = {10.1161/CIRCULATIONAHA.122.063481}, pages = {1518 -- 1533}, subject = {Toll-like receptor 3}, language = {en} }