@article{SchuhBanerjeeMosiaetal.,
  author    = {Schuh, Christina and Banerjee, Asmita and Mosia, Shorena and Hopf, Rudolf and Grasl, Christian and Schima, Heinrich and Schmidhammer, Robert and Redl, Heinz and R{\"u}nzler, Dominik and Morton, Tatjana J.},
  title     = {Activated Schwann-like cells guided by fibrin structures enhance Axonal Regeneration},
  series = {JOURNAL OF TISSUE ENGINEERING AND REGENERATIVE MEDICINE},
  volume    = {2012},
  journal   = {JOURNAL OF TISSUE ENGINEERING AND REGENERATIVE MEDICINE},
  number    = {vol. 6/no. 1},
  subject      = {Cells},
  language  = {en}
}
@article{SchneiderEnayatiGrasletal.,
  author    = {Schneider, Karl Heinrich and Enayati, Marjan and Grasl, Christian and Walter, Ingrid and Budinsky, Lubos and Zebic, Gabriel and Kaun, Christoph and Wagner, Anja and Kratochwill, Klaus and Redl, Heinz and Teuschl, Andreas and Podesser, Bruno K. and Bergmeister, Helga},
  title     = {Acellular vascular matrix grafts from human placenta chorion: Impact of ECM preservation on graft characteristics, protein composition and in vivo performance.},
  series = {Biomaterials},
  journal   = {Biomaterials},
  pages     = {14 -- 26},
  abstract  = {Small diameter vascular grafts from human placenta, decellularized with either Triton X-100 (Triton) or SDS and crosslinked with heparin were constructed and characterized. Graft biochemical properties, residual DNA, and protein composition were evaluated to compare the effect of the two detergents on graft matrix composition and structural alterations. Biocompatibility was tested in vitro by culturing the grafts with primary human macrophages and in vivo by subcutaneous implantation of graft conduits (n = 7 per group) into the flanks of nude rats. Subsequently, graft performance was evaluated using an aortic implantation model in Sprague Dawley rats (one month, n = 14). In situ graft imaging was performed using MRI angiography. Retrieved specimens were analyzed by electromyography, scanning electron microscopy, histology and immunohistochemistry to evaluate cell migration and the degree of functional tissue remodeling. Both decellularization methods resulted in grafts of excellent biocompatibility in vitro and in vivo, with low immunogenic potential. Proteomic data revealed removal of cytoplasmic proteins with relative enrichment of ECM proteins in decelluarized specimens of both groups. Noteworthy, LC-Mass Spectrometry analysis revealed that 16 proteins were exclusively preserved in Triton decellularized specimens in comparison to SDS-treated specimens. Aortic grafts showed high patency rates, no signs of thrombus formation, aneurysms or rupture. Conduits of both groups revealed tissue-specific cell migration indicative of functional remodeling. This study strongly suggests that decellularized allogenic grafts from the human placenta have the potential to be used as vascular replacement materials. Both detergents produced grafts with low residual immunogenicity and appropriate mechanical properties. Observed differences in graft characteristics due to preservation method had no impact on successful in vivo performance in the rodent model.},
  subject      = {Biomaterial},
  language  = {en}
}
@article{SchneiderRohringerKapelleretal.,
  author    = {Schneider, Karl and Rohringer, Sabrina and Kapeller, Barbara and Grasl, Christian and Kiss, Herbert and Heber, Stefan and Walter, Ingrid and Teuschl, Andreas and Podesser, Bruno K. and Bergmeister, Helga},
  title     = {Riboflavin-mediated photooxidation to improve the characteristics of decellularized human arterial small diameter vascular grafts},
  series = {Acta Biomater.},
  volume    = {2020},
  journal   = {Acta Biomater.},
  number    = {116},
  pages     = {246 -- 258},
  abstract  = {Vascular grafts with a diameter of less than 6 mm are made from a variety of materials and techniques to provide alternatives to autologous vascular grafts. Decellularized materials have been proposed as a possible approach to create extracellular matrix (ECM) vascular prostheses as they are naturally derived and inherently support various cell functions. However, these desirable graft characteristics may be limited by alterations of the ECM during the decellularization process leading to decreased biomechanical properties and hemocompatibility. In this study, arteries from the human placenta chorion were decellularized using two distinct detergents (Triton X-100 or SDS), which differently affect ECM ultrastructure. To overcome biomechanical strength loss and collagen fiber exposure after decellularization, riboflavin-mediated UV (RUV) crosslinking was used to uniformly crosslink the collagenous ECM of the grafts. Graft characteristics and biocompatibility with and without RUV crosslinking were studied in vitro and in vivo. RUV-crosslinked ECM grafts showed significantly improved mechanical strength and smoothening of the luminal graft surfaces. Cell seeding using human endothelial cells revealed no cytotoxic effects of the RUV treatment. Short-term aortic implants in rats showed cell migration and differentiation of host cells. Functional graft remodeling was evident in all grafts. Thus, RUV crosslinking is a preferable tool to improve graft characteristics of decellularized matrix conduits.},
  subject      = {Tissue Engineering},
  language  = {en}
}
@article{SchneiderRohringerKapelleretal.,
  author    = {Schneider, Karl H. and Rohringer, Sabrina and Kapeller, Barbara and Grasl, Christian and Kiss, Herbert and Heber, Stefan and Walter, Ingrid and Teuschl, Andreas H. and Podesser, Bruno K. and Bergmeister, Helga},
  title     = {Riboflavin-mediated photooxidation to improve the characteristics of decellularized human arterial small diameter vascular grafts},
  series = {Acta Biomaterialia},
  volume    = {116},
  journal   = {Acta Biomaterialia},
  pages     = {246 -- 258},
  abstract  = {Vascular grafts with a diameter of less than 6 mm are made from a variety of materials and techniques to provide alternatives to autologous vascular grafts. Decellularized materials have been proposed as a possible approach to create extracellular matrix (ECM) vascular prostheses as they are naturally derived and inherently support various cell functions. However, these desirable graft characteristics may be limited by alterations of the ECM during the decellularization process leading to decreased biomechanical properties and hemocompatibility. In this study, arteries from the human placenta chorion were decellularized using two distinct detergents (Triton X-100 or SDS), which differently affect ECM ultrastructure. To overcome biomechanical strength loss and collagen fiber exposure after decellularization, riboflavin-mediated UV (RUV) crosslinking was used to uniformly crosslink the collagenous ECM of the grafts. Graft characteristics and biocompatibility with and without RUV crosslinking were studied in vitro and in vivo. RUV-crosslinked ECM grafts showed significantly improved mechanical strength and smoothening of the luminal graft surfaces. Cell seeding using human endothelial cells revealed no cytotoxic effects of the RUV treatment. Short-term aortic implants in rats showed cell migration and differentiation of host cells. Functional graft remodeling was evident in all grafts. Thus, RUV crosslinking is a preferable tool to improve graft characteristics of decellularized matrix conduits.},
  subject      = {Tissue Engineering},
  language  = {en}
}