@article{BachmannSpitzRothbaueretal.,
  author    = {Bachmann, Barbara and Spitz, Sarah and Rothbauer, Mario and Jordan, Christian and Purtscher, Michaela and Zirath, Helene and Schuller, Patrick and Eilenberger, Christoph and Ali, Syed Faheem and M{\"u}hleder, Severin and Priglinger, Eleni and Harasek, Michael and Redl, Heinz and Holnthoner, Wolfgang and Ertl, Peter},
  title     = {Engineering of three-dimensional pre-vascular networks within fibrin hydrogel constructs by microfluidic control over reciprocal cell signaling},
  series = {Biomicrofluidics},
  journal   = {Biomicrofluidics},
  subject      = {Microfluidic},
  language  = {en}
}
@article{SchneiderPultarOesterreicheretal.,
  author    = {Schneider, Jaana and Pultar, Marianne and Oesterreicher, Johannes and Bobbili, Madhusudhan Reddy and M{\"u}hleder, Severin and Priglinger, Eleni and Redl, Heinz and Spittler, Andreas and Grillari, Johannes and Holnthoner, Wolfgang},
  title     = {Cre mRNA Is Not Transferred by EVs from Endothelial and Adipose-Derived Stromal/Stem Cells during Vascular Network Formation},
  series = {Int J Mol Sci.},
  volume    = {2021},
  journal   = {Int J Mol Sci.},
  number    = {22(8)},
  pages     = {4050},
  abstract  = {Coculture systems employing adipose tissue-derived mesenchymal stromal/stem cells (ASC) and endothelial cells (EC) represent a widely used technique to model vascularization. Within this system, cell-cell communication is crucial for the achievement of functional vascular network formation. Extracellular vesicles (EVs) have recently emerged as key players in cell communication by transferring bioactive molecules between cells. In this study we aimed to address the role of EVs in ASC/EC cocultures by discriminating between cells, which have received functional EV cargo from cells that have not. Therefore, we employed the Cre-loxP system, which is based on donor cells expressing the Cre recombinase, whose mRNA was previously shown to be packaged into EVs and reporter cells containing a construct of floxed dsRed upstream of the eGFP coding sequence. The evaluation of Cre induced color switch in the reporter system via EVs indicated that there is no EV-mediated RNA transmission either between EC themselves or EC and ASC. However, since Cre mRNA was not found present in EVs, it remains unclear if Cre mRNA is generally not packaged into EVs or if EVs are not taken up by the utilized cell types. Our data indicate that this technique may not be applicable to evaluate EV-mediated cell-to-cell communication in an in vitro setting using EC and ASC. Further investigations will require a functional system showing efficient and specific loading of Cre mRNA or protein into EVs.},
  subject      = {Tissue Engineering},
  language  = {en}
}
@article{StrohmeierHofmannJacaketal.,
  author    = {Strohmeier, Karin and Hofmann, Martina and Jacak, Jaroslaw and Narzt, Marie-Sophie and Wahlmueller, Marlene and Mairhofer, Mario and Sch{\"a}dl, Barbara and Holnthoner, Wolfgang and Barsch, Martin and Sandhofer, Matthias and Wolbank, Susanne and Priglinger, Eleni},
  title     = {Multi-Level Analysis of Adipose Tissue Reveals the Relevance of Perivascular Subpopulations and an Increased Endothelial Permeability in Early-Stage Lipedema},
  series = {Biomedicines},
  volume    = {2022},
  journal   = {Biomedicines},
  number    = {10(5)},
  pages     = {1163},
  abstract  = {Lipedema is a chronic, progressive disease of adipose tissue with unknown etiology. Based on the relevance of the stromal vascular fraction (SVF) cell population in lipedema, we performed a thorough characterization of subcutaneous adipose tissue, SVF isolated thereof and the sorted populations of endothelial cells (EC), pericytes and cultured adipose-derived stromal/stem cells (ASC) of early-stage lipedema patients. We employed histological and gene expression analysis and investigated the endothelial barrier by immunofluorescence and analysis of endothelial permeability in vitro. Although there were no significant differences in histological stainings, we found altered gene expression of factors relevant for local estrogen metabolism (aromatase), preadipocyte commitment (ZNF423) and immune cell infiltration (CD11c) in lipedema on the tissue level, as well as in distinct cellular subpopulations. Machine learning analysis of immunofluorescence images of CD31 and ZO-1 revealed a morphological difference in the cellular junctions of EC cultures derived from healthy and lipedema individuals. Furthermore, the secretome of lipedema-derived SVF cells was sufficient to significantly increase leakiness of healthy human primary EC, which was also reflected by decreased mRNA expression of VE-cadherin. Here, we showed for the first time that the secretome of SVF cells creates an environment that triggers endothelial barrier dysfunction in early-stage lipedema. Moreover, since alterations in gene expression were detected on the cellular and/or tissue level, the choice of sample material is of high importance in elucidating this complex disease.},
  subject      = {Tissue Engineering},
  language  = {en}
}
@article{HromadaHartmannOesterreicheretal.,
  author    = {Hromada, Carina and Hartmann, Jaana and Oesterreicher, Johannes and Stoiber, Anton and Daerr, Anna and Sch{\"a}dl, Barbara and Priglinger, Eleni and Teuschl-Woller, Andreas H. and Holnthoner, Wolfgang and Heinzel, Johannes Christoph and Hercher, David},
  title     = {Occurrence of Lymphangiogenesis in Peripheral Nerve Autografts Contrasts Schwann Cell-Induced Apoptosis of Lymphatic Endothelial Cells In Vitro},
  series = {Biomolecules},
  volume    = {2022},
  journal   = {Biomolecules},
  number    = {12, 6},
  pages     = {820},
  abstract  = {Peripheral nerve injuries pose a major clinical concern world-wide, and functional recovery after segmental peripheral nerve injury is often unsatisfactory, even in cases of autografting. Although it is well established that angiogenesis plays a pivotal role during nerve regeneration, the influence of lymphangiogenesis is strongly under-investigated. In this study, we analyzed the presence of lymphatic vasculature in healthy and regenerated murine peripheral nerves, revealing that nerve autografts contained increased numbers of lymphatic vessels after segmental damage. This led us to elucidate the interaction between lymphatic endothelial cells (LECs) and Schwann cells (SCs) in vitro. We show that SC and LEC secretomes did not influence the respective other cell types' migration and proliferation in 2D scratch assay experiments. Furthermore, we successfully created lymphatic microvascular structures in SC-embedded 3D fibrin hydrogels, in the presence of supporting cells; whereas SCs seemed to exert anti-lymphangiogenic effects when cultured with LECs alone. Here, we describe, for the first time, increased lymphangiogenesis after peripheral nerve injury and repair. Furthermore, our findings indicate a potential lymph-repellent property of SCs, thereby providing a possible explanation for the lack of lymphatic vessels in the healthy endoneurium. Our results highlight the importance of elucidating the molecular mechanisms of SC-LEC interaction.},
  subject      = {Tissue Engineering},
  language  = {en}
}