@article{TomaschMaleinerHeheretal., author = {Tomasch, Janine and Maleiner, Babette and Heher, Philipp and Rufin, Manuel and Andriotis, Orestis G. and Thurner, Philipp J. and Redl, Heinz and Fuchs, Christiane and Teuschl-Woller, Andreas H.}, title = {Changes in Elastic Moduli of Fibrin Hydrogels Within the Myogenic Range Alter Behavior of Murine C2C12 and Human C25 Myoblasts Differently}, series = {Froniers in Bioengineering and Biotechnology}, volume = {10}, journal = {Froniers in Bioengineering and Biotechnology}, pages = {836520}, abstract = {Fibrin hydrogels have proven highly suitable scaffold materials for skeletal muscle tissue engineering in the past. Certain parameters of those types of scaffolds, however, greatly affect cellular mechanobiology and therefore the myogenic outcome. The aim of this study was to identify the influence of apparent elastic properties of fibrin scaffolds in 2D and 3D on myoblasts and evaluate if those effects differ between murine and human cells. Therefore, myoblasts were cultured on fibrin-coated multiwell plates ("2D") or embedded in fibrin hydrogels ("3D") with different elastic moduli. Firstly, we established an almost linear correlation between hydrogels' fibrinogen concentrations and apparent elastic moduli in the range of 7.5 mg/ml to 30 mg/ml fibrinogen (corresponds to a range of 7.7-30.9 kPa). The effects of fibrin hydrogel elastic modulus on myoblast proliferation changed depending on culture type (2D vs 3D) with an inhibitory effect at higher fibrinogen concentrations in 3D gels and vice versa in 2D. The opposite effect was evident in differentiating myoblasts as shown by gene expression analysis of myogenesis marker genes and altered myotube morphology. Furthermore, culture in a 3D environment slowed down proliferation compared to 2D, with a significantly more pronounced effect on human myoblasts. Differentiation potential was also substantially impaired upon incorporation into 3D gels in human, but not in murine, myoblasts. With this study, we gained further insight in the influence of apparent elastic modulus and culture type on cellular behavior and myogenic outcome of skeletal muscle tissue engineering approaches. Furthermore, the results highlight the need to adapt parameters of 3D culture setups established for murine cells when applied to human cells.}, subject = {Tissue Engineering}, language = {en} } @misc{HeherTomaschMaleineretal., author = {Heher, Philipp and Tomasch, Janine and Maleiner, Babette and Redl, Heinz and Fuchs, Christiane}, title = {The Importance of Biomechanical Cues for In Vitro Skeletal Myogenesis}, subject = {In Vitro}, language = {en} } @article{SchuhHeherWeihsetal., author = {Schuh, Christina and Heher, Philipp and Weihs, Anna and Fuchs, Christiane and Gabriel, Christian and Wolbank, Susanne and Mittermayr, Rainer and Redl, Heinz and R{\"u}nzler, Dominik and Teuschl, Andreas}, title = {In vitro extracorporeal shock wave treatment enhances stemness and preserves multipotency of rat and human adipose-derived stem cells}, series = {Journal of Cytotherapy}, journal = {Journal of Cytotherapy}, subject = {Shockwave}, language = {en} } @article{DeiningerWagnerHeimeletal., author = {Deininger, Christian and Wagner, Andrea and Heimel, Patrick and Salzer, Elias and Monforte Vila, Xavier and Weißenbacher, Nadja and Grillari, Johannes and Redl, Heinz and Wichlas, Florian and Freude, Thomas and Tempfer, Herbert and Teuschl-Woller, Andreas and Traweger, Andreas}, title = {Enhanced BMP-2-Mediated Bone Repair Using an Anisotropic Silk Fibroin Scaffold Coated with Bone-like Apatite}, series = {Int. J. Mol. Sci.}, volume = {23}, journal = {Int. J. Mol. Sci.}, number = {1 / 283}, abstract = {The repair of large bone defects remains challenging and often requires graft material due to limited availability of autologous bone. In clinical settings, collagen sponges loaded with excessive amounts of bone morphogenetic protein 2 (rhBMP-2) are occasionally used for the treatment of bone non-unions, increasing the risk of adverse events. Therefore, strategies to reduce rhBMP-2 dosage are desirable. Silk scaffolds show great promise due to their favorable biocompatibility and their utility for various biofabrication methods. For this study, we generated silk scaffolds with axially aligned pores, which were subsequently treated with 10× simulated body fluid (SBF) to generate an apatitic calcium phosphate coating. Using a rat femoral critical sized defect model (CSD) we evaluated if the resulting scaffold allows the reduction of BMP-2 dosage to promote efficient bone repair by providing appropriate guidance cues. Highly porous, anisotropic silk scaffolds were produced, demonstrating good cytocompatibility in vitro and treatment with 10× SBF resulted in efficient surface coating. In vivo, the coated silk scaffolds loaded with a low dose of rhBMP-2 demonstrated significantly improved bone regeneration when compared to the unmineralized scaffold. Overall, our findings show that this simple and cost-efficient technique yields scaffolds that enhance rhBMP-2 mediated bone healing.}, subject = {Tissue Engineering}, language = {en} } @article{FarokhiAleemardaniSolouketal., author = {Farokhi, Maryam and Aleemardani, Mina and Solouk, Atefeh and Mirzadeh, Hamid and Teuschl, Andreas Herbert and Redl, Heinz}, title = {Crosslinking strategies for silk fibroin hydrogels: promising biomedical materials}, series = {Biomedical Materials}, volume = {16}, journal = {Biomedical Materials}, number = {2}, pages = {022004}, abstract = {Due to their strong biomimetic potential, silk fibroin (SF) hydrogels are impressive candidates for tissue engineering, due to their tunable mechanical properties, biocompatibility, low immunotoxicity, controllable biodegradability, and a remarkable capacity for biomaterial modification and the realization of a specific molecular structure. The fundamental chemical and physical structure of SF allows its structure to be altered using various crosslinking strategies. The established crosslinking methods enable the formation of three-dimensional (3D) networks under physiological conditions. There are different chemical and physical crosslinking mechanisms available for the generation of SF hydrogels (SFHs). These methods, either chemical or physical, change the structure of SF and improve its mechanical stability, although each method has its advantages and disadvantages. While chemical crosslinking agents guarantee the mechanical strength of SFH through the generation of covalent bonds, they could cause some toxicity, and their usage is not compatible with a cell-friendly technology. On the other hand, physical crosslinking approaches have been implemented in the absence of chemical solvents by the induction of β-sheet conformation in the SF structure. Unfortunately, it is not easy to control the shape and properties of SFHs when using this method. The current review discusses the different crosslinking mechanisms of SFH in detail, in order to support the development of engineered SFHs for biomedical applications.}, subject = {Tissue Engineering}, language = {en} } @article{SchandaKeiblHeimeletal., author = {Schanda, Jakob and Keibl, Claudia and Heimel, Patrick and Monforte, Xavier and Feichtinger, Xaver and Teuschl, Andreas and Baierl, Andreas and Muschitz, Christian and Redl, Heinz and Fialka, Christian and Mittermayr, Rainer}, title = {Zoledronic Acid Substantially Improves Bone Microarchitecture and Biomechanical Properties After Rotator Cuff Repair in a Rodent Chronic Defect Model}, series = {Am J Sports Med}, volume = {2020 Jul}, journal = {Am J Sports Med}, number = {48 (9)}, pages = {2151 -- 2160}, abstract = {Background: Bone mineral density at the humeral head is reduced in patients with chronic rotator cuff tears. Bone loss in the humeral head is associated with repair failure after rotator cuff reconstruction. Bisphosphonates (eg, zoledronic acid) increase bone mineral density. Hypothesis: Zoledronic acid improves bone mineral density of the humeral head and biomechanical properties of the enthesis after reconstruction of chronic rotator cuff tears in rats. Study design: Controlled laboratory study. Methods: A total of 32 male Sprague-Dawley rats underwent unilateral (left) supraspinatus tenotomy with delayed transosseous rotator cuff reconstruction after 3 weeks. All rats were sacrificed 8 weeks after rotator cuff repair. Animals were randomly assigned to 1 of 2 groups. At 1 day after rotator cuff reconstruction, the intervention group was treated with a single subcutaneous dose of zoledronic acid at 100 µg/kg bodyweight, and the control group received 1 mL of subcutaneous saline solution. In 12 animals of each group, micro-computed tomography scans of both shoulders were performed as well as biomechanical testing of the supraspinatus enthesis of both sides. In 4 animals of each group, histological analyses were conducted. Results: In the intervention group, bone volume fraction (bone volume/total volume [BV/TV]) of the operated side was higher at the lateral humeral head (P = .005) and the medial humeral head (P = .010) compared with the control group. Trabecular number on the operated side was higher at the lateral humeral head (P = .004) and the medial humeral head (P = .001) in the intervention group. Maximum load to failure rates on the operated side were higher in the intervention group (P < .001). Cortical thickness positively correlated with higher maximum load to failure rates in the intervention group (r = 0.69; P = .026). Histological assessment revealed increased bone formation in the intervention group. Conclusion: Single-dose therapy of zoledronic acid provided an improvement of bone microarchitecture at the humeral head as well as an increase of maximum load to failure rates after transosseous reconstruction of chronic rotator cuff lesions in rats. Clinical relevance: Zoledronic acid improves bone microarchitecture as well as biomechanical properties after reconstruction of chronic rotator cuff tears in rodents. These results need to be verified in clinical investigations.}, subject = {Tissue Engineering}, language = {en} } @article{BachmannSpitzSchaedletal., author = {Bachmann, Barbara and Spitz, Sarah and Sch{\"a}dl, Barbara and Teuschl, Andreas and Redl, Heinz and N{\"u}rnberger, Sylvia and Ertl, Peter}, title = {Stiffness Matters: Fine-Tuned Hydrogel Elasticity Alters Chondrogenic Redifferentiation}, series = {Froniers in Bioengineering and Biotechnology}, volume = {2020}, journal = {Froniers in Bioengineering and Biotechnology}, number = {8}, pages = {373}, abstract = {Biomechanical cues such as shear stress, stretching, compression, and matrix elasticity are vital in the establishment of next generation physiological in vitro tissue models. Matrix elasticity, for instance, is known to guide stem cell differentiation, influence healing processes and modulate extracellular matrix (ECM) deposition needed for tissue development and maintenance. To better understand the biomechanical effect of matrix elasticity on the formation of articular cartilage analogs in vitro, this study aims at assessing the redifferentiation capacity of primary human chondrocytes in three different hydrogel matrices of predefined matrix elasticities. The hydrogel elasticities were chosen to represent a broad spectrum of tissue stiffness ranging from very soft tissues with a Young's modulus of 1 kPa up to elasticities of 30 kPa, representative of the perichondral-space. In addition, the interplay of matrix elasticity and transforming growth factor beta-3 (TGF-β3) on the redifferentiation of primary human articular chondrocytes was studied by analyzing both qualitative (viability, morphology, histology) and quantitative (RT-qPCR, sGAG, DNA) parameters, crucial to the chondrotypic phenotype. Results show that fibrin hydrogels of 30 kPa Young's modulus best guide chondrocyte redifferentiation resulting in a native-like morphology as well as induces the synthesis of physiologic ECM constituents such as glycosaminoglycans (sGAG) and collagen type II. This comprehensive study sheds light onto the mechanobiological impact of matrix elasticity on formation and maintenance of articular cartilage and thus represents a major step toward meeting the need for advanced in vitro tissue models to study both re- and degeneration of articular cartilage.}, subject = {Tissue Engineering}, language = {en} } @article{FeichtingerMonforteKeibletal., author = {Feichtinger, Xaver and Monforte, Xavier and Keibl, Claudia and Hercher, David and Schanda, Jakob and Teuschl, Andreas and Muschitz, Christian and Redl, Heinz and Fialka, Christian and Mittermayr, Rainer}, title = {Substantial Biomechanical Improvement by Extracorporeal Shockwave Therapy After Surgical Repair of Rodent Chronic Rotator Cuff Tears.}, series = {American Journal of Sports Medicine}, journal = {American Journal of Sports Medicine}, subject = {Shockwave Therapy}, language = {en} } @article{TeuschlTanglHeimeletal., author = {Teuschl, Andreas and Tangl, Stefan and Heimel, Patrick and Schwarze, Uwe Yacine and Monforte, Xavier and Redl, Heinz and Nau, Thomas}, title = {Osteointegration of a Novel Silk Fiber-Based ACL Scaffold by Formation of a Ligament-Bone Interface.}, series = {American Journal of Sports Medicine}, journal = {American Journal of Sports Medicine}, subject = {Tissue Engineering}, language = {en} } @misc{TeuschlSchuhWeihsetal., author = {Teuschl, Andreas and Schuh, Christina and Weihs, Anna and Guillaume, Olivier and Monforte Vila, Xavier and Redl, Heinz and Kaplan, David and R{\"u}nzler, Dominik}, title = {Tailoring bioactivity of silk-based biomaterials via delivering and functionalization strategies with fibrinogen/thrombin, plant lectins or laminin}, subject = {Biomaterials}, language = {en} } @article{SlezakSlezakHartingeretal., author = {Slezak, Paul and Slezak, Cyrill and Hartinger, Joachim and Teuschl, Andreas and N{\"u}rnberger, Sylvia and Redl, Heinz and Mittermayr, Rainer}, title = {A Low Cost Implantation Model in the Rat That Allows a Spatial Assessment of Angiogenesis.}, series = {Frontiers in Bioengineering and Biotechnology}, journal = {Frontiers in Bioengineering and Biotechnology}, abstract = {There is continual demand for animal models that allow a quantitative assessment of angiogenic properties of biomaterials, therapies, and pharmaceuticals. In its simplest form, this is done by subcutaneous material implantation and subsequent vessel counting which usually omits spatial data. We have refined an implantation model and paired it with a computational analytic routine which outputs not only vessel count but also vessel density, distribution, and vessel penetration depth, that relies on a centric vessel as a reference point. We have successfully validated our model by characterizing the angiogenic potential of a fibrin matrix in conjunction with recombinant human vascular endothelial growth factor (rhVEGF165). The inferior epigastric vascular pedicles of rats were sheathed with silicone tubes, which were subsequently filled with 0.2 ml of fibrin and different doses of rhVEGF165, centrically embedding the vessels. Over 4 weeks, tissue samples were harvested and subsequently immunohistologically stained and computationally analyzed. The model was able to detect variations over the angiogenic potentials of growth factor spiked fibrin matrices. Adding 20 ng of rhVEGF165 resulted in a significant increase in vasculature while 200 ng of rhVEGF165 did not improve vascular growth. Vascularized tissue volume increased during the first week and vascular density increased during the second week. Total vessel count increased significantly and exhibited a peak after 2 weeks which was followed by a resorption of vasculature by week 4. In summary, a simple implantation model to study in vivo vascularization with only a minimal workload attached was enhanced to include morphologic data of the emerging vascular tree.}, subject = {Tissue Engineering}, language = {en} } @article{BerkovitchCohenPeledetal., author = {Berkovitch, Yulia and Cohen, Talia and Peled, Eli and Schmidhammer, Robert and Hildner, Florian and Teuschl, Andreas and Wolbank, Susanne and Yelin, Dvir and Redl, Heinz and Seliktar, Dror}, title = {Hydrogel composition and laser micropatterning to regulate sciatic nerve regeneration.}, series = {Journal of Tissue Engineering and Regenerative Medicine}, journal = {Journal of Tissue Engineering and Regenerative Medicine}, pages = {1049 -- 1061}, abstract = {Treatment of peripheral nerve injuries has evolved over the past several decades to include the use of sophisticated new materials endowed with trophic and topographical cues that are essential for in vivo nerve fibre regeneration. In this research, we explored the use of an advanced design strategy for peripheral nerve repair, using biological and semi-synthetic hydrogels that enable controlled environmental stimuli to regenerate neurons and glial cells in a rat sciatic nerve resection model. The provisional nerve growth conduits were composed of either natural fibrin or adducts of synthetic polyethylene glycol and fibrinogen or gelatin. A photo-patterning technique was further applied to these 3D hydrogel biomaterials, in the form of laser-ablated microchannels, to provide contact guidance for unidirectional growth following sciatic nerve injury. We tested the regeneration capacity of subcritical nerve gap injuries in rats treated with photo-patterned materials and compared these with injuries treated with unpatterned hydrogels, either stiff or compliant. Among the factors tested were shear modulus, biological composition, and micropatterning of the materials. The microchannel guidance patterns, combined with appropriately matched degradation and stiffness properties of the material, proved most essential for the uniform tissue propagation during the nerve regeneration process.}, subject = {Tissue Engineering}, language = {en} } @misc{TeuschlHeimelMonforteVilaetal., author = {Teuschl, Andreas and Heimel, Patrick and Monforte Vila, Xavier and N{\"u}rnberger, Sylvia and Tangl, Stefan and van Griensven, Martijn and Redl, Heinz and Nau, Thomas}, title = {Anterior cruciate ligament regeneration using the silk-based RegACL scaffold}, subject = {Tissue Engineering}, language = {en} } @incollection{HackethalSchuhHoferetal., author = {Hackethal, Johannes and Schuh, Christina and Hofer, Alexandra and Meixner, Barbara and Hennerbichler, Simone and Redl, Heinz and Teuschl, Andreas}, title = {Human Placenta Laminin-111 as a Multifunctional Protein for Tissue Engineering and Regenerative Medicine}, series = {Advances in Experimental Medicine and Biology}, booktitle = {Advances in Experimental Medicine and Biology}, publisher = {Springer}, publisher = {Fachhochschule Technikum Wien}, subject = {Biomaterial}, language = {en} } @misc{SlezakRoseHercheretal., author = {Slezak, Paul and Rose, Roland and Hercher, David and Weihs, Anna and Fuchs, Christiane and Redl, Heinz and Mittermayr, Rainer and Slezak, Cyrill}, title = {Tracking therapeutic shockwaves and their impact on regeneration}, subject = {Shockwave Therapy}, language = {en} } @article{HackethalMuehlederHoferetal., author = {Hackethal, Johannes and M{\"u}hleder, Severin and Hofer, Alexandra and Schneider, Karl Heinrich and Pr{\"u}ller, Johanna and Hennerbichler, Simone and Redl, Heinz and Teuschl, Andreas}, title = {An Effective Method of Atelocollagen Type 1/3 Isolation from Human Placenta and Its In Vitro Characterization in Two-Dimensional and Three-Dimensional Cell Culture Applications}, series = {Tissue Eng Part C Methods}, volume = {23}, journal = {Tissue Eng Part C Methods}, number = {5}, pages = {274 -- 285}, subject = {Placenta}, language = {en} } @article{BerkovitchCohenPeledetal., author = {Berkovitch, Yulia and Cohen, Talia and Peled, Eli and Schmidhammer, Robert and Hildner, Florian and Teuschl, Andreas and Wolbank, Susanne and Yelin, Dvir and Redl, Heinz and Seliktar, Dror}, title = {Hydrogel Composition and Laser Micro-Patterning to Regulate Sciatic Nerve Regeneration}, series = {Journal of Tissue Engineering and Regenerative Medicine}, volume = {12}, journal = {Journal of Tissue Engineering and Regenerative Medicine}, number = {4}, subject = {Micro-Patterning}, language = {en} } @article{BernhardFergusonRiederetal., author = {Bernhard, Jonathan and Ferguson, James and Rieder, Bernhard and Heimel, Patrick and Nau, Thomas and Tangl, Stefan and Redl, Heinz and Vunjak-Novakovic, Gordana}, title = {Tissue-engineered hypertrophic chondrocyte grafts enhanced long bone repair biomaterials}, series = {Biomaterials}, journal = {Biomaterials}, number = {139}, pages = {202 -- 212}, subject = {Grafting}, language = {en} } @article{SchneiderLehmannvanOschetal., author = {Schneider, Cornelia and Lehmann, Johannes and van Osch, Gerjo and Hildner, Florian and Teuschl, Andreas and Monforte Vila, Xavier and Miosga, David and Heimel, Patrick and Priglinger, Eleni and Redl, Heinz and Wolbank, Susanne and N{\"u}rnberger, Sylvia}, title = {Systematic Comparison of Protocols for the Preparation of Human Articular Cartilage for Use as Scaffold Material in Cartilage Tissue Engineering}, series = {Tissue Eng Part C Methods}, volume = {22}, journal = {Tissue Eng Part C Methods}, number = {12}, subject = {Cartilage}, language = {en} } @inproceedings{MaleinerHeherTeuschletal., author = {Maleiner, Babette and Heher, Philipp and Teuschl, Andreas and Redl, Heinz and R{\"u}nzler, Dominik and Fuchs, Christiane}, title = {Generation of aligned skeletal muscle-like tissue based on the application of strain to a 3D fibrin scaffold}, series = {Proceedings of PACT "Designer Cells go Clinical" Symposium}, booktitle = {Proceedings of PACT "Designer Cells go Clinical" Symposium}, subject = {Tissue Generation}, language = {en} } @misc{MaleinerHeherTeuschletal., author = {Maleiner, Babette and Heher, Philipp and Teuschl, Andreas and Redl, Heinz and R{\"u}nzler, Dominik and Fuchs, Christiane}, title = {Generation of aligned skeletal muscle-like tissue based on the application of strain to a 3D fibrin scaffold}, subject = {Tissue Generation}, language = {en} } @misc{TeuschlFuchsWeihsetal., author = {Teuschl, Andreas and Fuchs, Christiane and Weihs, Anna and Heimel, Patrick and R{\"u}nzler, Dominik and Redl, Heinz and Nau, Thomas}, title = {The Silk Road from Textiles to Novel Medical Implants}, subject = {Silk}, language = {en} } @misc{TeuschlHeimelNuernbergeretal., author = {Teuschl, Andreas and Heimel, Patrick and N{\"u}rnberger, Sylvia and Redl, Heinz and Nau, Thomas}, title = {ACL Regeneration using a novel silk fiber based scaffold - Histological Results of a Large Animal Study}, subject = {Tissue Regeneration}, language = {en} } @inproceedings{BasoliChaudryCrucianietal., author = {Basoli, Valentina and Chaudry, Sidrah and Cruciani, Sara and Fuchs, Christiane and Rieger, Sabine and Dungel, Peter and Wolbank, Susanne and Ventura, Carlo and Grillari-Voglauer, Regina and Redl, Heinz and Maioli, Margherita}, title = {Epigenetic and molecular behavious of stem cells exposed to biophysical stimuli: new insights in regenerative medicine}, series = {Proceedings des Seminars zum 40. Jahresjubil{\"a}um der {\"O}sterreichischen Gesellschaft f{\"u}r Chirurgische Forschung}, booktitle = {Proceedings des Seminars zum 40. Jahresjubil{\"a}um der {\"O}sterreichischen Gesellschaft f{\"u}r Chirurgische Forschung}, subject = {Stem Cells}, language = {en} } @misc{BasoliChaudryCrucianietal., author = {Basoli, Valentina and Chaudry, Sidrah and Cruciani, Gabriele and Fuchs, Christiane and Rieger, Sabine and Dungel, Peter and Wolbank, Susanne and Ventura, Carlo and Grillari-Voglauer, Regina and Redl, Heinz and Maioli, Margherita}, title = {Epigenetic and molecular behavious of stem cells exposed to biophysical stimuli: new insights in regenerative medicine}, subject = {Stem Cells}, language = {en} } @article{HeherMaleinerPruelleretal., author = {Heher, Philipp and Maleiner, Babette and Pr{\"u}ller, Johanna and Teuschl, Andreas and Kollmitzer, Josef and Monforte Vila, Xavier and Wolbank, Susanne and Redl, Heinz and R{\"u}nzler, Dominik and Fuchs, Christiane}, title = {A novel bioreactor for the generation of highly aligned 3D skeletal muscle-like constructs through orientation of fibrin via application of static strain}, series = {Acta Biomaterialia}, journal = {Acta Biomaterialia}, subject = {Bioreactor}, language = {en} } @article{TeuschlSchuhHalbweisetal., author = {Teuschl, Andreas and Schuh, Christina and Halbweis, Robert and Pajer, Krisztian and Marton, Gabor and Hopf, Rudolf and Mosia, Shorena and R{\"u}nzler, Dominik and Redl, Heinz and Nogradi, Antal and Hausner, Thomas}, title = {A new preparation method for anisotropic silk fibroin nerve guidance conduits and its evaluation in vitro and in a rat sciatic nerve defect model}, series = {Tissue Engineering Part C: Methods}, journal = {Tissue Engineering Part C: Methods}, subject = {Fibrin}, language = {en} } @misc{TeuschlSchuhHalbweisetal., author = {Teuschl, Andreas and Schuh, Christina and Halbweis, Robert and Marton, G{\´a}bor and Pajer, Kriszti{\´a}n and Hopf, Rudolf and Mosia, Shorena and R{\"u}nzler, Dominik and N{\´o}gr{\´a}di, Antal and Hausner, Thomas and Redl, Heinz}, title = {Silk fibroin for peripheral nerve regeneration: a novel preparation method improved mechanical characteristics and supports regeneration in rat sciatic nerves}, subject = {Fibroin}, language = {en} } @misc{FuchsWeihsTeuschletal., author = {Fuchs, Christiane and Weihs, Anna and Teuschl, Andreas and Hartinger, Joachim and Slezak, Paul and Mittermayr, Rainer and Redl, Heinz and Junger, Wolfgang and Sitte, Harald and R{\"u}nzler, Dominik}, title = {Shockwave Treatment Enhances Proliferation and Improves Wound Healing via Purinergic Signaling Linked ERK 1/2 Pathways}, subject = {Shockwave treatment}, language = {en} } @misc{FuchsWeihsTeuschletal., author = {Fuchs, Christiane and Weihs, Anna and Teuschl, Andreas and Hartinger, Joachim and Slezak, Paul and Mittermayr, Rainer and Redl, Heinz and Junger, Wolfgang and Sitte, Harald and R{\"u}nzler, Dominik}, title = {Shockwave Treatment Augments Proliferation and Improves Wound Healing via Purinergic Signaling Linked ERK 1/2 Pathways}, subject = {Shockwave treatment}, language = {en} } @misc{HeherFuchsPruelleretal., author = {Heher, Philipp and Fuchs, Christiane and Pr{\"u}ller, Johanna and Babette, Maleiner and R{\"u}nzler, Dominik and Redl, Heinz}, title = {A novel bioreactor for engineering skeletal muscle-like tissue in a strained fibrin scaffold}, subject = {Bioreactor}, language = {en} } @article{GuillaumeParkMonforteVilaetal., author = {Guillaume, Olivier and Park, Jaesung and Monforte Vila, Xavier and Gruber-Blum, Simone and Redl, Heinz and Petter-Puchner, Akexander and Teuschl, Andreas}, title = {Fabrication of silk mesh with enhanced cytocompatibility: preliminary in vitro investigation toward cell-based therapy for hernia repair}, series = {Journal of Materials Science: Materials in Medicine}, journal = {Journal of Materials Science: Materials in Medicine}, subject = {Cytocompatibility}, language = {en} } @misc{WeihsFuchsTeuschletal., author = {Weihs, Anna and Fuchs, Christiane and Teuschl, Andreas and Hartinger, Joachim and Slezak, Paul and Mittermayr, Rainer and Redl, Heinz and Junger, Wolfgang and Sitte, Harald and R{\"u}nzler, Dominik}, title = {Shockwave treatment activates Erk1/2 pathways predominantly via P2Y receptor involvement}, subject = {Shockwave}, language = {en} } @article{TeuschlHeimelNuernbergeretal., author = {Teuschl, Andreas and Heimel, Patrick and N{\"u}rnberger, Sylvia and van Griensven, Martijn and Redl, Heinz and Nau, Thomas}, title = {A Novel Silk Fiber-Based Scaffold for Regeneration of the Anterior Cruciate Ligament: Histological Results From a Study in Sheep.}, series = {The American Journal of Sports Medicine}, journal = {The American Journal of Sports Medicine}, subject = {Ligament}, language = {en} } @misc{TeuschlFuchsFeichtingeretal., author = {Teuschl, Andreas and Fuchs, Christiane and Feichtinger, Georg and Heher, Philipp and Heimel, Patrick and Schuh, Christina and N{\"u}rnberger, Sylvia and Nau, Thomas and R{\"u}nzler, Dominik and Redl, Heinz}, title = {Fibrin or Fibroin - Not only the "o" Makes the Difference}, subject = {Fibrin}, language = {en} } @article{TeuschlBalmayorRedletal., author = {Teuschl, Andreas and Balmayor, Elizabeth and Redl, Heinz and van Griensven, Martijn and Dungel, Peter}, title = {Phototherapy With LED Light Modulates Healing Processes in an In Vitro Scratch Wound-Model Using 3 Different Cell Types}, series = {Dermatologic Surgery}, volume = {41}, journal = {Dermatologic Surgery}, number = {2}, pages = {261 -- 268}, subject = {Phototherapy}, language = {en} } @article{SchuhHercherStaineretal., author = {Schuh, Christina and Hercher, David and Stainer, Michaela and Hopf, Rudolf and Teuschl, Andreas and Schmidhammer, Robert and Redl, Heinz}, title = {Extracorporeal shockwave treatment: A novel tool to improve Schwann cell isolation and culture}, series = {Cytotherapy}, journal = {Cytotherapy}, subject = {Extracorporeal shockwave}, language = {en} } @misc{TeuschlNuernbergerHeimeletal., author = {Teuschl, Andreas and N{\"u}rnberger, Sylvia and Heimel, Patrick and Redl, Heinz and Nau, Thomas}, title = {Regeneration of the Anterior Cruciate Ligament Using a Silk-Fiber Based Scaffold - Histological Results}, subject = {Tissue Regeneration}, language = {en} } @article{NauRedlTeuschl, author = {Nau, Thomas and Redl, Heinz and Teuschl, Andreas}, title = {Comment on: In Vivo Evaluation of Electrospun Polycaprolactone Graft for Anterior Cruciate Ligament Engineering}, series = {Tissue Engineering Part A}, journal = {Tissue Engineering Part A}, subject = {Grafting}, language = {en} } @article{WeihsFuchsTeuschletal., author = {Weihs, Anna and Fuchs, Christiane and Teuschl, Andreas and Hartinger, Joachim and Slezak, Paul and Mittermayr, Rainer and Redl, Heinz and Junger, Wolfgang and Sitte, Harald and R{\"u}nzler, Dominik}, title = {Shock Wave Treatment Enhances Cell Proliferation and Improves Wound Healing by ATP Release-coupled Extracellular Signal-regulated Kinase (ERK) Activation}, series = {The Journal of biological chemistry}, journal = {The Journal of biological chemistry}, subject = {Shockwave}, language = {de} } @article{SchuhHeherWeihsetal., author = {Schuh, Christina and Heher, Philipp and Weihs, Anna and Asmita, Banerjee and Wolbank, Susanne and Mittermayr, Rainer and Redl, Heinz and R{\"u}nzler, Dominik and Teuschl, Andreas}, title = {Adipose derived stem cells respond to in vitro extracorporeal shockwave treatment with increased stemness and multipotency}, series = {New Biotechnology}, journal = {New Biotechnology}, subject = {Shockwave}, language = {en} } @misc{HeherFuchsPruelleretal., author = {Heher, Philipp and Fuchs, Christiane and Pr{\"u}ller, Johanna and Maleiner, Babette and Kollmitzer, Josef and R{\"u}nzler, Dominik and Teuschl, Andreas and Wolbank, Susanne and Redl, Heinz}, title = {A bioreactor-based 3D culture system for skeletal muscle engineering in fibrin scaffolds}, subject = {Bioreactors}, language = {en} } @article{BanerjeeNuernbergerHennerbichleretal., author = {Banerjee, Asmita and N{\"u}rnberger, Sylvia and Hennerbichler, Simone and Riedl, Stefan and Schuh, Christina and Hacobian, Ara and Teuschl, Andreas and Eibl, J{\"u}rgen and Redl, Heinz}, title = {In toto differentiation of human amniotic membrane towards the Schwann cell lineage}, series = {227-239}, volume = {15}, journal = {227-239}, number = {2}, subject = {Membrane}, language = {en} } @article{TeuschlNeutschMonforteVilaetal., author = {Teuschl, Andreas and Neutsch, Lukas and Monforte Vila, Xavier and R{\"u}nzler, Dominik and van Griensven, Martijn and Gabor, Franz and Redl, Heinz}, title = {Enhanced cell adhesion on silk fibroin via lectin surface modification.}, series = {Acta Biomaterialia}, journal = {Acta Biomaterialia}, subject = {Silk}, language = {en} } @article{HohlriederTeuschlCichaetal., author = {Hohlrieder, Manfred and Teuschl, Andreas and Cicha, Klaus and van Griensven, Martijn and Redl, Heinz and Stampfl, J{\"u}rgen}, title = {Bioreactor and scaffold design for the mechanical stimulation of anterior cruciate ligament grafts}, series = {Biomedical materials and engineering}, volume = {23}, journal = {Biomedical materials and engineering}, number = {3}, pages = {225 -- 237}, subject = {Bioreactors}, language = {en} } @article{DungelTeuschlBanerjeeetal., author = {Dungel, Peter and Teuschl, Andreas and Banerjee, Asmita and Paier-Pourani, Jamile and Redl, Heinz and Kozlov, Andrey}, title = {Impact of mitochondria on nitrite metabolism in HL-1 cardiomyocytes}, series = {Frontiers in Physiology}, journal = {Frontiers in Physiology}, number = {4}, subject = {Nitrite}, language = {en} } @article{TeuschlNuernbergerRedletal., author = {Teuschl, Andreas and N{\"u}rnberger, Sylvia and Redl, Heinz and Nau, Thomas}, title = {Articular cartilage tissue regeneration: current research strategies and outlook for the future}, series = {European Surgery}, volume = {45}, journal = {European Surgery}, number = {3}, pages = {142 -- 153}, subject = {Tissue Regeneration}, language = {en} } @article{TeuschlvanGriensvenRedl, author = {Teuschl, Andreas and van Griensven, Martijn and Redl, Heinz}, title = {Sericin removal from raw Bombys mori silk scaffolds of high hierarchical order}, series = {Tissue Eng Part C Methods}, journal = {Tissue Eng Part C Methods}, subject = {Scaffold}, language = {en} } @article{SchuhBanerjeeMosiaetal., author = {Schuh, Christina and Banerjee, Asmita and Mosia, Shorena and Hopf, Rudolf and Grasl, Christian and Schima, Heinrich and Schmidhammer, Robert and Redl, Heinz and R{\"u}nzler, Dominik and Morton, Tatjana J.}, title = {Activated Schwann-like cells guided by fibrin structures enhance Axonal Regeneration}, series = {JOURNAL OF TISSUE ENGINEERING AND REGENERATIVE MEDICINE}, volume = {2012}, journal = {JOURNAL OF TISSUE ENGINEERING AND REGENERATIVE MEDICINE}, number = {vol. 6/no. 1}, subject = {Cells}, language = {en} } @article{RiederWeihsTeuschletal., author = {Rieder, Bernhard and Weihs, Anna and Teuschl, Andreas and Knebl, Gerald and Kollmitzer, Josef and Redl, Heinz and R{\"u}nzler, Dominik}, title = {Evaluation of cell response on permanent and pulsed atmospheric pressure stressed cells}, series = {Journal of Tissue Engineering and Regenerative Medicine}, volume = {1}, journal = {Journal of Tissue Engineering and Regenerative Medicine}, number = {6}, pages = {240 -- 240}, subject = {Cells}, language = {en} } @article{MartinMKollmitzerRedletal., author = {Martin M., Frank and Kollmitzer, Josef and Redl, Heinz and R{\"u}nzler, Dominik}, title = {Shear force stimulation of adipose-tissue derived stem cells in a novel bioreactor}, series = {Journal of Tissue Engineering and Regenerative Medicine}, volume = {1}, journal = {Journal of Tissue Engineering and Regenerative Medicine}, number = {6}, pages = {340 -- 340}, subject = {Stem Cells}, language = {en} } @inproceedings{WeihsKneblRedletal., author = {Weihs, Anna and Knebl, Gerald and Redl, Heinz and R{\"u}nzler, Dominik}, title = {Evaluation of cell migration methods in 3D hydrogels for tissue engineering applications}, series = {3. Forschungsforum der {\"o}sterreichischen Fachhochschulen / Fachhochschule K{\"a}rnten}, booktitle = {3. Forschungsforum der {\"o}sterreichischen Fachhochschulen / Fachhochschule K{\"a}rnten}, isbn = {978-3-853912850}, pages = {490 -- 491}, subject = {Cells}, language = {en} } @inproceedings{KneblWeihsWeingantetal., author = {Knebl, Gerald and Weihs, Anna and Weingant, Michaela and Steininger, Thomas and Redl, Heinz and R{\"u}nzler, Dominik}, title = {Automatisierte Datenauswertung eines Boyden-Mikro-Chemotaxis-Kammer Zell-Migrations Assays.}, series = {2. Forschungsforum der {\"o}sterreichischen Fachhochschulen}, booktitle = {2. Forschungsforum der {\"o}sterreichischen Fachhochschulen}, isbn = {978-3-8322-7023-0}, pages = {412 -- 417}, subject = {Cells}, language = {de} } @inproceedings{RuenzlerKneblPeterbaueretal., author = {R{\"u}nzler, Dominik and Knebl, Gerald and Peterbauer, A. and Wolbank, Susanne and Morton, Tatjana J. and Redl, Heinz}, title = {Darstellung fluoreszenzmarkierter adulter Stammzellen in drei-dimensionaler Zellkultur mittels Konfokaler Laser Scanning Mikroskopie}, series = {Erstes Forschungsforum der {\"o}sterreichischen Fachhochschulen}, booktitle = {Erstes Forschungsforum der {\"o}sterreichischen Fachhochschulen}, pages = {311 -- 316}, subject = {Cells}, language = {en} } @article{TeuschlAignerHohlriederetal., author = {Teuschl, Andreas and Aigner, Elmar and Hohlrieder, Martin and Cicha, Klaus and Stampfl, J{\"u}rgen and Redl, Heinz}, title = {Stimulation of ligament tissue formation on a silk scaffold with mechanical loading using a custom-made bioreactor system}, series = {Journal of Tissue Engineering and Regenerative Medicine}, volume = {1}, journal = {Journal of Tissue Engineering and Regenerative Medicine}, number = {6}, pages = {51 -- 51}, subject = {Ligament}, language = {en} } @article{TeuschlFergusonSzomolanyietal., author = {Teuschl, Andreas and Ferguson, James and Szomolanyi, Pavol and Trattnig, Siegfried and Redl, Heinz and Nau, Thomas}, title = {Osteointegration of anterior cruciate ligament scaffolds fabricated of bombyx mori silk}, series = {Journal of Tissue Engineering and Regenerative Medicine}, volume = {1}, journal = {Journal of Tissue Engineering and Regenerative Medicine}, number = {6}, pages = {181 -- 182}, subject = {Osteointegration}, language = {en} } @article{RiederWeihsWeidingeretal., author = {Rieder, Bernhard and Weihs, Anna and Weidinger, Adelheid and Sczwarc, Dorota and N{\"u}rnberger, Sylvia and Redl, Heinz and R{\"u}nzler, Dominik and Huber-Gries, Carina and Teuschl, Andreas}, title = {Hydrostatic pressure-generated reactive oxygen species induce osteoarthritic conditions in cartilage pellet cultures}, series = {Scientific Reports}, journal = {Scientific Reports}, subject = {Bioreactor}, language = {en} } @misc{MuehlederFuchsBassilioetal., author = {M{\"u}hleder, Severin and Fuchs, Christiane and Bassilio, Jose and Sczwarc, Dorota and Pill, Karoline and Slezak, Paul and Labuda, Krystina and Siehs, Christian and Pr{\"o}ll, Johannes and Priglinger, Eleni and Redl, Heinz and Holnthoner, Wolfgang}, title = {The purinergic receptor P2Y2 modulates endothelial sprouting and angiogenesis}, subject = {Angiogenesis}, language = {en} } @article{NuernbergerSchneiderKeibletal., author = {N{\"u}rnberger, S. and Schneider, C. and Keibl, C. and Sch{\"a}dl, Barbara and Heimel, P. and Monforte, X. and Teuschl, A. H. and Nalbach, M. and Thurner, P. J. and Grillari, J. and Redl, Heinz and Wolbank, S.}, title = {Repopulation of decellularised articular cartilage by laser-based matrix engraving}, series = {EBioMedicine.}, volume = {64}, journal = {EBioMedicine.}, number = {103196.}, abstract = {Background: In spite of advances in the treatment of cartilage defects using cell and scaffold-based therapeutic strategies, the long-term outcome is still not satisfying since clinical scores decline years after treatment. Scaffold materials currently used in clinical settings have shown limitations in providing suitable biomechanical properties and an authentic and protective environment for regenerative cells. To tackle this problem, we developed a scaffold material based on decellularised human articular cartilage. Methods: Human articular cartilage matrix was engraved using a CO2 laser and treated for decellularisation and glycosaminoglycan removal. Characterisation of the resulting scaffold was performed via mechanical testing, DNA and GAG quantification and in vitro cultivation with adipose-derived stromal cells (ASC). Cell vitality, adhesion and chondrogenic differentiation were assessed. An ectopic, unloaded mouse model was used for the assessment of the in vivo performance of the scaffold in combination with ASC and human as well as bovine chondrocytes. The novel scaffold was compared to a commercial collagen type I/III scaffold. Findings: Crossed line engravings of the matrix allowed for a most regular and ubiquitous distribution of cells and chemical as well as enzymatic matrix treatment was performed to increase cell adhesion. The biomechanical characteristics of this novel scaffold that we term CartiScaff were found to be superior to those of commercially available materials. Neo-tissue was integrated excellently into the scaffold matrix and new collagen fibres were guided by the laser incisions towards a vertical alignment, a typical feature of native cartilage important for nutrition and biomechanics. In an ectopic, unloaded in vivo model, chondrocytes and mesenchymal stromal cells differentiated within the incisions despite the lack of growth factors and load, indicating a strong chondrogenic microenvironment within the scaffold incisions. Cells, most noticeably bone marrow-derived cells, were able to repopulate the empty chondrocyte lacunae inside the scaffold matrix. Interpretation: Due to the better load-bearing, its chondrogenic effect and the ability to guide matrix-deposition, CartiScaff is a promising biomaterial to accelerate rehabilitation and to improve long term clinical success of cartilage defect treatment. Funding: Austrian Research Promotion Agency FFG ("CartiScaff" \#842455), Lorenz B{\"o}hler Fonds (16/13), City of Vienna Competence Team Project Signaltissue (MA23, \#18-08). Keywords: Cartilage regeneration; Decellularisation; Ectopic animal model; Laser engraving; Mechanical testing; Repopulation.}, subject = {Tissue Engineering}, language = {en} } @article{SchneiderEnayatiGrasletal., author = {Schneider, Karl Heinrich and Enayati, Marjan and Grasl, Christian and Walter, Ingrid and Budinsky, Lubos and Zebic, Gabriel and Kaun, Christoph and Wagner, Anja and Kratochwill, Klaus and Redl, Heinz and Teuschl, Andreas and Podesser, Bruno K. and Bergmeister, Helga}, title = {Acellular vascular matrix grafts from human placenta chorion: Impact of ECM preservation on graft characteristics, protein composition and in vivo performance.}, series = {Biomaterials}, journal = {Biomaterials}, pages = {14 -- 26}, abstract = {Small diameter vascular grafts from human placenta, decellularized with either Triton X-100 (Triton) or SDS and crosslinked with heparin were constructed and characterized. Graft biochemical properties, residual DNA, and protein composition were evaluated to compare the effect of the two detergents on graft matrix composition and structural alterations. Biocompatibility was tested in vitro by culturing the grafts with primary human macrophages and in vivo by subcutaneous implantation of graft conduits (n = 7 per group) into the flanks of nude rats. Subsequently, graft performance was evaluated using an aortic implantation model in Sprague Dawley rats (one month, n = 14). In situ graft imaging was performed using MRI angiography. Retrieved specimens were analyzed by electromyography, scanning electron microscopy, histology and immunohistochemistry to evaluate cell migration and the degree of functional tissue remodeling. Both decellularization methods resulted in grafts of excellent biocompatibility in vitro and in vivo, with low immunogenic potential. Proteomic data revealed removal of cytoplasmic proteins with relative enrichment of ECM proteins in decelluarized specimens of both groups. Noteworthy, LC-Mass Spectrometry analysis revealed that 16 proteins were exclusively preserved in Triton decellularized specimens in comparison to SDS-treated specimens. Aortic grafts showed high patency rates, no signs of thrombus formation, aneurysms or rupture. Conduits of both groups revealed tissue-specific cell migration indicative of functional remodeling. This study strongly suggests that decellularized allogenic grafts from the human placenta have the potential to be used as vascular replacement materials. Both detergents produced grafts with low residual immunogenicity and appropriate mechanical properties. Observed differences in graft characteristics due to preservation method had no impact on successful in vivo performance in the rodent model.}, subject = {Biomaterial}, language = {en} } @article{PriglingerSchuhSteffenhagenetal., author = {Priglinger, Eleni and Schuh, Christina and Steffenhagen, Carolin and Wurzer, Christoph and Maier, Julia and N{\"u}rnberger, Sylvia and Holnthoner, Wolfgang and Fuchs, Christiane and Suessner, Susanne and R{\"u}nzler, Dominik and Redl, Heinz and Wolbank, Susanne}, title = {Improvement of adipose tissue-derived cells by low-energy extracorporeal shock wave therapy.}, series = {Cytotherapy}, journal = {Cytotherapy}, pages = {1079 -- 1095}, abstract = {BACKGROUND: Cell-based therapies with autologous adipose tissue-derived cells have shown great potential in several clinical studies in the last decades. The majority of these studies have been using the stromal vascular fraction (SVF), a heterogeneous mixture of fibroblasts, lymphocytes, monocytes/macrophages, endothelial cells, endothelial progenitor cells, pericytes and adipose-derived stromal/stem cells (ASC) among others. Although possible clinical applications of autologous adipose tissue-derived cells are manifold, they are limited by insufficient uniformity in cell identity and regenerative potency. METHODS: In our experimental set-up, low-energy extracorporeal shock wave therapy (ESWT) was performed on freshly obtained human adipose tissue and isolated adipose tissue SVF cells aiming to equalize and enhance stem cell properties and functionality. RESULTS: After ESWT on adipose tissue we could achieve higher cellular adenosine triphosphate (ATP) levels compared with ESWT on the isolated SVF as well as the control. ESWT on adipose tissue resulted in a significantly higher expression of single mesenchymal and vascular marker compared with untreated control. Analysis of SVF protein secretome revealed a significant enhancement in insulin-like growth factor (IGF)-1 and placental growth factor (PLGF) after ESWT on adipose tissue. DISCUSSION: Summarizing we could show that ESWT on adipose tissue enhanced the cellular ATP content and modified the expression of single mesenchymal and vascular marker, and thus potentially provides a more regenerative cell population. Because the effectiveness of autologous cell therapy is dependent on the therapeutic potency of the patient's cells, this technology might raise the number of patients eligible for autologous cell transplantation.}, subject = {Shockwave Therapy}, language = {en} } @article{BachmannSpitzRothbaueretal., author = {Bachmann, Barbara and Spitz, Sarah and Rothbauer, Mario and Jordan, Christian and Purtscher, Michaela and Zirath, Helene and Schuller, Patrick and Eilenberger, Christoph and Ali, Syed Faheem and M{\"u}hleder, Severin and Priglinger, Eleni and Harasek, Michael and Redl, Heinz and Holnthoner, Wolfgang and Ertl, Peter}, title = {Engineering of three-dimensional pre-vascular networks within fibrin hydrogel constructs by microfluidic control over reciprocal cell signaling}, series = {Biomicrofluidics}, journal = {Biomicrofluidics}, subject = {Microfluidic}, language = {en} } @misc{SchneiderAignerMonforteVilaetal., author = {Schneider, Karl Heinrich and Aigner, Petra and Monforte Vila, Xavier and Holnthoner, Wolfgang and Teuschl, Andreas and Bergmeister, Helga and Redl, Heinz}, title = {Naturally derived acellular small diameter vascular grafts from human placenta for reconstructive surgery}, subject = {Placenta}, language = {en} } @article{SchneiderPultarOesterreicheretal., author = {Schneider, Jaana and Pultar, Marianne and Oesterreicher, Johannes and Bobbili, Madhusudhan Reddy and M{\"u}hleder, Severin and Priglinger, Eleni and Redl, Heinz and Spittler, Andreas and Grillari, Johannes and Holnthoner, Wolfgang}, title = {Cre mRNA Is Not Transferred by EVs from Endothelial and Adipose-Derived Stromal/Stem Cells during Vascular Network Formation}, series = {Int J Mol Sci.}, volume = {2021}, journal = {Int J Mol Sci.}, number = {22(8)}, pages = {4050}, abstract = {Coculture systems employing adipose tissue-derived mesenchymal stromal/stem cells (ASC) and endothelial cells (EC) represent a widely used technique to model vascularization. Within this system, cell-cell communication is crucial for the achievement of functional vascular network formation. Extracellular vesicles (EVs) have recently emerged as key players in cell communication by transferring bioactive molecules between cells. In this study we aimed to address the role of EVs in ASC/EC cocultures by discriminating between cells, which have received functional EV cargo from cells that have not. Therefore, we employed the Cre-loxP system, which is based on donor cells expressing the Cre recombinase, whose mRNA was previously shown to be packaged into EVs and reporter cells containing a construct of floxed dsRed upstream of the eGFP coding sequence. The evaluation of Cre induced color switch in the reporter system via EVs indicated that there is no EV-mediated RNA transmission either between EC themselves or EC and ASC. However, since Cre mRNA was not found present in EVs, it remains unclear if Cre mRNA is generally not packaged into EVs or if EVs are not taken up by the utilized cell types. Our data indicate that this technique may not be applicable to evaluate EV-mediated cell-to-cell communication in an in vitro setting using EC and ASC. Further investigations will require a functional system showing efficient and specific loading of Cre mRNA or protein into EVs.}, subject = {Tissue Engineering}, language = {en} } @article{NuernbergerSchneidervanOschetal., author = {N{\"u}rnberger, Sylvia and Schneider, Cornelia and van Osch, Gerjo and Keibl, Claudia and Rieder, Bernhard and Monforte, Xavier and Teuschl, Andreas and M{\"u}hleder, Severin and Holnthoner, Wolfgang and Sch{\"a}dl, Barbara and Gahleitner, Christoph and Redl, Heinz and Wolbank, Susanne}, title = {Repopulation of an auricular cartilage scaffold, AuriScaff, perforated with an enzyme combination.}, series = {Acta Biomaterialia}, journal = {Acta Biomaterialia}, subject = {Tissue Engineering}, language = {en} } @article{JohannesWeihsKarneretal., author = {Johannes, Hackethal and Weihs, Anna and Karner, Lisa and Metzger, Magdalena and Dungel, Peter and Hennerbichler, Simone and Redl, Heinz and Teuschl-Woller, Andreas Herbert}, title = {Novel Human Placenta-Based Extract for Vascularization Strategies in Tissue Engineering}, series = {Tissue Eng Part C Methods}, volume = {27}, journal = {Tissue Eng Part C Methods}, number = {11}, pages = {616 -- 632}, abstract = {There is critical unmet need for new vascularized tissues to support or replace injured tissues and organs. Various synthetic and natural materials were already established for use of two-dimensional (2D) and three-dimensional (3D) in vitro neovascularization assays, however, they still cannot mimic the complex functions of the sum of the extracellular matrix (ECM) in native intact tissue. Currently, this issue is only addressed by artificial products such as Matrigel™, which comprises a complex mixture of ECM proteins, extracted from animal tumor tissue. Despite its outstanding bioactivity, the isolation from tumor tissue hinders its translation into clinical applications. Since nonhuman ECM proteins may cause immune reactions, as are frequently observed in clinical trials, human ECM proteins represent the best option when aiming for clinical applications. Here, we describe an effective method of isolating a human placenta substrate (hpS) that induces the spontaneous formation of an interconnected network of green fluorescence-labeled human umbilical vein endothelial cells (gfpHUVECs) in vitro. The substrate was biochemically characterized by using a combination of bicinchoninic acid (BCA) assay, DNA, and glycosaminoglycan (GAG) content assays, sodium dodecyl sulfate/polyacrylamide gel electrophoresis (SDS-PAGE) analysis and Western blot, angiogenesis arrays, chromatographic thrombin detection, high performance liquid chromatography (HPLC)-based amino acid quantification analysis, and assessment of antimicrobial properties. 2D in vitro cell culture experiments have been performed to determine the vasculogenic potential of hpS, which demonstrated that cell networks developed on hpS show a significantly higher degree of complexity (number of tubules/junctions; total/mean tube length) when compared with Matrigel. As 3D cell culture techniques represent a more accurate representation of the in vivo condition, the substrate was 3D solidified using various natural polymers. 3D in vitro vasculogenesis assays have been performed by seeding gfpHUVECs in an hpS-fibrinogen clot. In conclusion, hpS provides a potent human/material-based alternative to xenogenic-material-based biomaterials for vascularization strategies in tissue engineering.}, subject = {Tissue Engineering}, language = {en} } @inproceedings{KneblMortonRedletal., author = {Knebl, Gerald and Morton, Tatjana J. and Redl, Heinz and R{\"u}nzler, Dominik}, title = {Mechanical stimulation of cells in 3-dimensional fibrin constructs using a bioreactor}, series = {3. Forschungsforum der {\"o}sterreichischen Fachhochschulen / Fachhochschule K{\"a}rnten}, booktitle = {3. Forschungsforum der {\"o}sterreichischen Fachhochschulen / Fachhochschule K{\"a}rnten}, pages = {492 -- 493}, subject = {Cells}, language = {en} } @article{SchneiderAignerHolnthoneretal., author = {Schneider, Karl Heinrich and Aigner, Petra and Holnthoner, Wolfgang and Monforte Vila, Xavier and N{\"u}rnberger, Sylvia and R{\"u}nzler, Dominik and Redl, Heinz and Teuschl, Andreas}, title = {Decellularized human placenta chorion matrix as a favorable source of small-diameter vascular grafts}, series = {Acta Biomaterialia}, journal = {Acta Biomaterialia}, subject = {Grafting}, language = {en} } @article{HeimelSwiadekSlezaketal., author = {Heimel, Patrick and Swiadek, Nicole V. and Slezak, Paul and Kerbl, Markus and Schneider, Cornelia and N{\"u}rnberger, Sylvia and Redl, Heinz and Teuschl, Andreas and Hercher, David}, title = {Iodine-Enhanced Micro-CT Imaging of Soft Tissue on the Example of Peripheral Nerve Regeneration}, series = {Contrast Media \& Molecular Imaging}, journal = {Contrast Media \& Molecular Imaging}, subject = {µCT}, language = {en} } @article{RohringerHolnthonerHackletal., author = {Rohringer, Sabrina and Holnthoner, Wolfgang and Hackl, Matthias and Weihs, Anna and R{\"u}nzler, Dominik and Skalicky, Susanna and Karbiener, Michael and Scheideler, Marcel and Pr{\"o}ll, Johannes and Gabriel, Christian and Schweighofer, Bernhard and Gr{\"o}ger, Marion and Spittler, Andreas and Grillari, Johannes and Redl, Heinz}, title = {Molecular and cellular effects of in vitro shockwave treatment on lymphatic endothelial cells.}, series = {PLoS one}, journal = {PLoS one}, subject = {Shockwave}, language = {en} } @article{RothbauerByrneSchobesbergeretal., author = {Rothbauer, Mario and Byrne, Ruth A. and Schobesberger, Silvia and Olmos Calvo, Isabel and Fischer, Anita and Reihs, Eva I. and Spitz, Sarah and Bachmann, Barbara and Sevelda, Florian and Holinka, Johannes and Holnthoner, Wolfgang and Redl, Heinz and Toegel, Stefan and Windhager, Reinhard and Kiener, Hans P. and Ertl, Peter}, title = {Establishment of a human three-dimensional chip-based chondro-synovial coculture joint model for reciprocal cross talk studies in arthritis research}, series = {Lab on a Chip}, volume = {2021}, journal = {Lab on a Chip}, number = {21}, pages = {4128 -- 4143}, abstract = {Rheumatoid arthritis is characterised by a progressive, intermittent inflammation at the synovial membrane, which ultimately leads to the destruction of the synovial joint. The synovial membrane as the joint capsule's inner layer is lined with fibroblast-like synoviocytes that are the key player supporting persistent arthritis leading to bone erosion and cartilage destruction. While microfluidic models that model molecular aspects of bone erosion between bone-derived cells and synoviocytes have been established, RA's synovial-chondral axis has not yet been realised using a microfluidic 3D model based on human patient in vitro cultures. Consequently, we established a chip-based three-dimensional tissue coculture model that simulates the reciprocal cross talk between individual synovial and chondral organoids. When co-cultivated with synovial organoids, we could demonstrate that chondral organoids induce a higher degree of cartilage physiology and architecture and show differential cytokine response compared to their respective monocultures highlighting the importance of reciprocal tissue-level cross talk in the modelling of arthritic diseases.}, subject = {Tissue Engineering}, language = {en} } @article{AshmwePosaRuehrnoessletal., author = {Ashmwe, Mohamed and Posa, Katja and R{\"u}hrn{\"o}ßl, Alexander and Heinzel, Johannes Christoph and Heimel, Patrick and Mock, Michael and Sch{\"a}dl, Barbara and Keibl, Claudia and Couillard-Despres, Sebastien and Redl, Heinz and Mittermayr, Rainer and Hercher, David}, title = {Effects of Extracorporeal Shockwave Therapy on Functional Recovery and Circulating miR-375 and miR-382-5p after Subacute and Chronic Spinal Cord Contusion Injury in Rats}, series = {Biomedicines}, volume = {2022}, journal = {Biomedicines}, number = {10(7)}, doi = {https://doi.org/10.3390/biomedicines10071630}, pages = {1630}, abstract = {Extracorporeal shockwave therapy (ESWT) can stimulate processes to promote regeneration, including cell proliferation and modulation of inflammation. Specific miRNA expression panels have been established to define correlations with regulatory targets within these pathways. This study aims to investigate the influence of low-energy ESWT-applied within the subacute and chronic phase of SCI (spinal cord injury) on recovery in a rat spinal cord contusion model. Outcomes were evaluated by gait analysis, µCT and histological analysis of spinal cords. A panel of serum-derived miRNAs after SCI and after ESWT was investigated to identify injury-, regeneration- and treatment-associated expression patterns. Rats receiving ESWT showed significant improvement in motor function in both a subacute and a chronic experimental setting. This effect was not reflected in changes in morphology, µCT-parameters or histological markers after ESWT. Expression analysis of various miRNAs, however, revealed changes after SCI and ESWT, with increased miR-375, indicating a neuroprotective effect, and decreased miR-382-5p potentially improving neuroplasticity via its regulatory involvement with BDNF. We were able to demonstrate a functional improvement of ESWT-treated animals after SCI in a subacute and chronic setting. Furthermore, the identification of miR-375 and miR-382-5p could potentially provide new targets for therapeutic intervention in future studies.}, subject = {Tissue Engineering}, language = {en} } @article{HanetsederLevstekTeuschlWolleretal., author = {Hanetseder, Dominik and Levstek, Tina and Teuschl-Woller, Andreas and Frank, Julia Katharina and Schaedl, Barbara and Redl, Heinz and Marolt Presen, Darja}, title = {Engineering of extracellular matrix from human iPSC-mesenchymal progenitors to enhance osteogenic capacity of human bone marrow stromal cells independent of their age}, series = {Front Bioeng Biotechnol}, volume = {11}, journal = {Front Bioeng Biotechnol}, doi = {https://doi.org/10.3389/fbioe.2023.1214019}, abstract = {Regeneration of bone defects is often limited due to compromised bone tissue physiology. Previous studies suggest that engineered extracellular matrices enhance the regenerative capacity of mesenchymal stromal cells. In this study, we used human-induced pluripotent stem cells, a scalable source of young mesenchymal progenitors (hiPSC-MPs), to generate extracellular matrix (iECM) and test its effects on the osteogenic capacity of human bone-marrow mesenchymal stromal cells (BMSCs). iECM was deposited as a layer on cell culture dishes and into three-dimensional (3D) silk-based spongy scaffolds. After decellularization, iECM maintained inherent structural proteins including collagens, fibronectin and laminin, and contained minimal residual DNA. Young adult and aged BMSCs cultured on the iECM layer in osteogenic medium exhibited a significant increase in proliferation, osteogenic marker expression, and mineralization as compared to tissue culture plastic. With BMSCs from aged donors, matrix mineralization was only detected when cultured on iECM, but not on tissue culture plastic. When cultured in 3D iECM/silk scaffolds, BMSCs exhibited significantly increased osteogenic gene expression levels and bone matrix deposition. iECM layer showed a similar enhancement of aged BMSC proliferation, osteogenic gene expression, and mineralization compared with extracellular matrix layers derived from young adult or aged BMSCs. However, iECM increased osteogenic differentiation and decreased adipocyte formation compared with single protein substrates including collagen and fibronectin. Together, our data suggest that the microenvironment comprised of iECM can enhance the osteogenic activity of BMSCs, providing a bioactive and scalable biomaterial strategy for enhancing bone regeneration in patients with delayed or failed bone healing.}, subject = {aging}, language = {en} } @article{BernhardMaroltPresenLietal., author = {Bernhard, Jonathan C and Marolt Presen, Darja and Li, Ming and Monforte, Xavier and Ferguson, James and Leinfellner, Gabriele and Heimel, Patrick and Betti, Susanne L and Shu, Sharon and Teuschl-Woller, Andreas H and Tangl, Stefan and Redl, Heinz and Vunjak-Novakovic, Gordana}, title = {Effects of Endochondral and Intramembranous Ossification Pathways on Bone Tissue Formation and Vascularization in Human Tissue-Engineered Grafts}, series = {Cells}, volume = {11}, journal = {Cells}, number = {19:3070}, doi = {10.3390/cells11193070}, abstract = {Bone grafts can be engineered by differentiating human mesenchymal stromal cells (MSCs) via the endochondral and intramembranous ossification pathways. We evaluated the effects of each pathway on the properties of engineered bone grafts and their capacity to drive bone regeneration. Bone-marrow-derived MSCs were differentiated on silk scaffolds into either hypertrophic chondrocytes (hyper) or osteoblasts (osteo) over 5 weeks of in vitro cultivation, and were implanted subcutaneously for 12 weeks. The pathways' constructs were evaluated over time with respect to gene expression, composition, histomorphology, microstructure, vascularization and biomechanics. Hypertrophic chondrocytes expressed higher levels of osteogenic genes and deposited significantly more bone mineral and proteins than the osteoblasts. Before implantation, the mineral in the hyper group was less mature than that in the osteo group. Following 12 weeks of implantation, the hyper group had increased mineral density but a similar overall mineral composition compared with the osteo group. The hyper group also displayed significantly more blood vessel infiltration than the osteo group. Both groups contained M2 macrophages, indicating bone regeneration. These data suggest that, similar to the body's repair processes, endochondral pathway might be more advantageous when regenerating large defects, whereas intramembranous ossification could be utilized to guide the tissue formation pattern with a scaffold architecture.}, subject = {bone tissue engineering}, language = {en} } @article{FeichtingerHeimelTangletal., author = {Feichtinger, Xaver and Heimel, Patrick and Tangl, Stefan and Keibl, Claudia and N{\"u}rnberger, Sylvia and Schanda, Jakob Emanuel and Hercher, David and Kocijan, Roland and Redl, Heinz and Grillari, Johannes and Fialka, Christian and Mittermayr, Rainer}, title = {Improved biomechanics in experimental chronic rotator cuff repair after shockwaves is not reflected by bone microarchitecture}, series = {PLoS One}, volume = {17}, journal = {PLoS One}, number = {1}, doi = {10.1371/journal.pone.0262294}, subject = {chronic rotator cuff repair}, language = {en} }