@misc{TeuschlFuchs,
  author    = {Teuschl, Andreas and Fuchs, Christiane},
  title     = {Bioreactors in Musculoskeletal Tissue Engineering},
  subject      = {Bioreactor},
  language  = {en}
}
@article{SchuhHeherWeihsetal.,
  author    = {Schuh, Christina and Heher, Philipp and Weihs, Anna and Fuchs, Christiane and Gabriel, Christian and Wolbank, Susanne and Mittermayr, Rainer and Redl, Heinz and R{\"u}nzler, Dominik and Teuschl, Andreas},
  title     = {In vitro extracorporeal shock wave treatment enhances stemness and preserves multipotency of rat and human adipose-derived stem cells},
  series = {Journal of Cytotherapy},
  journal   = {Journal of Cytotherapy},
  subject      = {Shockwave},
  language  = {en}
}
@article{HackethalDungelTeuschl,
  author    = {Hackethal, Johannes and Dungel, Peter and Teuschl, Andreas Herbert},
  title     = {Frequently Used Strategies to Isolate Extracellular Matrix Proteins from Human Placenta and Adipose Tissue},
  series = {Tissue Engineering Part C: Methods},
  volume    = {27},
  journal   = {Tissue Engineering Part C: Methods},
  number    = {12},
  pages     = {649 -- 660},
  abstract  = {The natural extracellular matrix (ECM) provides the optimal environment for cells. Many enzymatic or non-enzymatic based strategies to extract ECM proteins from tissues were published over the past years. However, every single isolation strategy reported so far is associated with specific bottlenecks. In this study, frequently used strategies to isolate ECM from human placenta or adipose tissue using Tris-, serum-, or pepsin-based buffers were compared. The resulting ECM proteins were biochemically characterized by analysis of cellular remnants using Hoechst DNA staining, glycosaminoglycan (GAG) content by dimethylmethylene blue, visualization of protein bands using sodium dodecyl sulfate-polyacrylamide gel electrophoresis analysis combined with amino acid quantification, and assessment of the proangiogenic profile using an angiogenesis array. Tris-NaCl-extracted ECM proteins showed a high heterogenic degree of extracted proteins, bioactive growth factors, and GAGs, but no collagen-I. Active serum-extracted ECM showed significant lower DNA remnants when compared with the Tris-NaCl isolation strategy. Pepsin-extracted ECM was rich in collagen-I and low amounts of remaining bioactive growth factors. This strategy was most effective to reduce DNA amounts when compared with the other isolation strategies. Pepsin-extracted ECM from both tissues easily gelled at 37°C, whereas the other extracted ECM strategies did not gel at 37°C (Tris-NaCl: liquid; serum: sponge). All relevant characteristics (DNA residues, ECM diversity and bioactivity, shape) of the extracted ECM proteins highly depend on its isolation strategy and could still be optimized. Impact statement The natural human extracellular matrix (ECM) is the ideal cell niche. Various strategies were reported to isolate human ECM components from various sources. In this article, we compared frequently used methods and compared their characteristics (DNA remnants, glycosaminoglycan content, sodium dodecyl sulfate-polyacrylamide gel electrophoresis analysis, amino acid quantification, angiogenesis array, and gel formation). We conclude that more research is still necessary to optimize current isolation approaches for in vitro or in vivo applications of human ECM.},
  subject      = {Tissue Engineering},
  language  = {en}
}
@article{KhimichProsolovMishurovaetal.,
  author    = {Khimich, Margarita A. and Prosolov, Konstantin A. and Mishurova, Tatiana and Evsevleev, Sergej and Monforte, Xavier and Teuschl, Andreas H. and Slezak, Paul and Ibragimov, Egor A. and Saprykin, Alexander A. and Kovalevskaya, Zhanna G. and Dmitriev, Andrey I. and Bruno, Giovanni and Sharkeev, Yurii P.},
  title     = {Advances in Laser Additive Manufacturing of Ti-Nb Alloys: From Nanostructured Powders to Bulk Objects},
  series = {Nanomaterials (Basel)},
  volume    = {11},
  journal   = {Nanomaterials (Basel)},
  number    = {5 / 1159},
  abstract  = {The additive manufacturing of low elastic modulus alloys that have a certain level of porosity for biomedical needs is a growing area of research. Here, we show the results of manufacturing of porous and dense samples by a laser powder bed fusion (LPBF) of Ti-Nb alloy, using two distinctive fusion strategies. The nanostructured Ti-Nb alloy powders were produced by mechanical alloying and have a nanostructured state with nanosized grains up to 90 nm. The manufactured porous samples have pronounced open porosity and advanced roughness, contrary to dense samples with a relatively smooth surface profile. The structure of both types of samples after LPBF is formed by uniaxial grains having micro- and nanosized features. The inner structure of the porous samples is comprised of an open interconnected system of pores. The volume fraction of isolated porosity is 2 vol. \% and the total porosity is 20 vol. \%. Cell viability was assessed in vitro for 3 and 7 days using the MG63 cell line. With longer culture periods, cells showed an increased cell density over the entire surface of a porous Ti-Nb sample. Both types of samples are not cytotoxic and could be used for further in vivo studies.},
  subject      = {Tissue Engineering},
  language  = {en}
}
@article{FarokhiAleemardaniSolouketal.,
  author    = {Farokhi, Maryam and Aleemardani, Mina and Solouk, Atefeh and Mirzadeh, Hamid and Teuschl, Andreas Herbert and Redl, Heinz},
  title     = {Crosslinking strategies for silk fibroin hydrogels: promising biomedical materials},
  series = {Biomedical Materials},
  volume    = {16},
  journal   = {Biomedical Materials},
  number    = {2},
  pages     = {022004},
  abstract  = {Due to their strong biomimetic potential, silk fibroin (SF) hydrogels are impressive candidates for tissue engineering, due to their tunable mechanical properties, biocompatibility, low immunotoxicity, controllable biodegradability, and a remarkable capacity for biomaterial modification and the realization of a specific molecular structure. The fundamental chemical and physical structure of SF allows its structure to be altered using various crosslinking strategies. The established crosslinking methods enable the formation of three-dimensional (3D) networks under physiological conditions. There are different chemical and physical crosslinking mechanisms available for the generation of SF hydrogels (SFHs). These methods, either chemical or physical, change the structure of SF and improve its mechanical stability, although each method has its advantages and disadvantages. While chemical crosslinking agents guarantee the mechanical strength of SFH through the generation of covalent bonds, they could cause some toxicity, and their usage is not compatible with a cell-friendly technology. On the other hand, physical crosslinking approaches have been implemented in the absence of chemical solvents by the induction of β-sheet conformation in the SF structure. Unfortunately, it is not easy to control the shape and properties of SFHs when using this method. The current review discusses the different crosslinking mechanisms of SFH in detail, in order to support the development of engineered SFHs for biomedical applications.},
  subject      = {Tissue Engineering},
  language  = {en}
}
@article{SchandaKeiblHeimeletal.,
  author    = {Schanda, Jakob and Keibl, Claudia and Heimel, Patrick and Monforte, Xavier and Feichtinger, Xaver and Teuschl, Andreas and Baierl, Andreas and Muschitz, Christian and Redl, Heinz and Fialka, Christian and Mittermayr, Rainer},
  title     = {Zoledronic Acid Substantially Improves Bone Microarchitecture and Biomechanical Properties After Rotator Cuff Repair in a Rodent Chronic Defect Model},
  series = {Am J Sports Med},
  volume    = {2020 Jul},
  journal   = {Am J Sports Med},
  number    = {48 (9)},
  pages     = {2151 -- 2160},
  abstract  = {Background: Bone mineral density at the humeral head is reduced in patients with chronic rotator cuff tears. Bone loss in the humeral head is associated with repair failure after rotator cuff reconstruction. Bisphosphonates (eg, zoledronic acid) increase bone mineral density. Hypothesis: Zoledronic acid improves bone mineral density of the humeral head and biomechanical properties of the enthesis after reconstruction of chronic rotator cuff tears in rats. Study design: Controlled laboratory study. Methods: A total of 32 male Sprague-Dawley rats underwent unilateral (left) supraspinatus tenotomy with delayed transosseous rotator cuff reconstruction after 3 weeks. All rats were sacrificed 8 weeks after rotator cuff repair. Animals were randomly assigned to 1 of 2 groups. At 1 day after rotator cuff reconstruction, the intervention group was treated with a single subcutaneous dose of zoledronic acid at 100 µg/kg bodyweight, and the control group received 1 mL of subcutaneous saline solution. In 12 animals of each group, micro-computed tomography scans of both shoulders were performed as well as biomechanical testing of the supraspinatus enthesis of both sides. In 4 animals of each group, histological analyses were conducted. Results: In the intervention group, bone volume fraction (bone volume/total volume [BV/TV]) of the operated side was higher at the lateral humeral head (P = .005) and the medial humeral head (P = .010) compared with the control group. Trabecular number on the operated side was higher at the lateral humeral head (P = .004) and the medial humeral head (P = .001) in the intervention group. Maximum load to failure rates on the operated side were higher in the intervention group (P < .001). Cortical thickness positively correlated with higher maximum load to failure rates in the intervention group (r = 0.69; P = .026). Histological assessment revealed increased bone formation in the intervention group. Conclusion: Single-dose therapy of zoledronic acid provided an improvement of bone microarchitecture at the humeral head as well as an increase of maximum load to failure rates after transosseous reconstruction of chronic rotator cuff lesions in rats. Clinical relevance: Zoledronic acid improves bone microarchitecture as well as biomechanical properties after reconstruction of chronic rotator cuff tears in rodents. These results need to be verified in clinical investigations.},
  subject      = {Tissue Engineering},
  language  = {en}
}
@article{BachmannSpitzSchaedletal.,
  author    = {Bachmann, Barbara and Spitz, Sarah and Sch{\"a}dl, Barbara and Teuschl, Andreas and Redl, Heinz and N{\"u}rnberger, Sylvia and Ertl, Peter},
  title     = {Stiffness Matters: Fine-Tuned Hydrogel Elasticity Alters Chondrogenic Redifferentiation},
  series = {Froniers in Bioengineering and Biotechnology},
  volume    = {2020},
  journal   = {Froniers in Bioengineering and Biotechnology},
  number    = {8},
  pages     = {373},
  abstract  = {Biomechanical cues such as shear stress, stretching, compression, and matrix elasticity are vital in the establishment of next generation physiological in vitro tissue models. Matrix elasticity, for instance, is known to guide stem cell differentiation, influence healing processes and modulate extracellular matrix (ECM) deposition needed for tissue development and maintenance. To better understand the biomechanical effect of matrix elasticity on the formation of articular cartilage analogs in vitro, this study aims at assessing the redifferentiation capacity of primary human chondrocytes in three different hydrogel matrices of predefined matrix elasticities. The hydrogel elasticities were chosen to represent a broad spectrum of tissue stiffness ranging from very soft tissues with a Young's modulus of 1 kPa up to elasticities of 30 kPa, representative of the perichondral-space. In addition, the interplay of matrix elasticity and transforming growth factor beta-3 (TGF-β3) on the redifferentiation of primary human articular chondrocytes was studied by analyzing both qualitative (viability, morphology, histology) and quantitative (RT-qPCR, sGAG, DNA) parameters, crucial to the chondrotypic phenotype. Results show that fibrin hydrogels of 30 kPa Young's modulus best guide chondrocyte redifferentiation resulting in a native-like morphology as well as induces the synthesis of physiologic ECM constituents such as glycosaminoglycans (sGAG) and collagen type II. This comprehensive study sheds light onto the mechanobiological impact of matrix elasticity on formation and maintenance of articular cartilage and thus represents a major step toward meeting the need for advanced in vitro tissue models to study both re- and degeneration of articular cartilage.},
  subject      = {Tissue Engineering},
  language  = {en}
}
@article{ZiadlouRotmanTeuschletal.,
  author    = {Ziadlou, Reihane and Rotman, Stijn and Teuschl, Andreas and Salzer, Elias and Barbero, Andrea and Martin, Ivan and Alini, Mauro and Eglin, David and Grad, Sibylle},
  title     = {Optimization of hyaluronic acid-tyramine/silk-fibroin composite hydrogels for cartilage tissue engineering and delivery of anti-inflammatory and anabolic drugs},
  series = {Materials Science and Engineering: C},
  volume    = {120},
  journal   = {Materials Science and Engineering: C},
  number    = {111701},
  abstract  = {Injury of articular cartilage leads to an imbalance in tissue homeostasis, and due to the poor self-healing capacity of cartilage the affected tissue often exhibits osteoarthritic changes. In recent years, injectable and highly tunable composite hydrogels for cartilage tissue engineering and drug delivery have been introduced as a desirable alternative to invasive treatments. In this study, we aimed to formulate injectable hydrogels for drug delivery and cartilage tissue engineering by combining different concentrations of hyaluronic acid-tyramine (HA-Tyr) with regenerated silk-fibroin (SF) solutions. Upon enzymatic crosslinking, the gelation and mechanical properties were characterized over time. To evaluate the effect of the hydrogel compositions and properties on extracellular matrix (ECM) deposition, bovine chondrocytes were embedded in enzymatically crosslinked HA-Tyr/SF composites (in further work abbreviated as HA/SF) or HA-Tyr hydrogels. We demonstrated that all hydrogel formulations were cytocompatible and could promote the expression of cartilage matrix proteins allowing chondrocytes to produce ECM, while the most prominent chondrogenic effects were observed in hydrogels with HA20/SF80 polymeric ratios. Unconfined mechanical testing showed that the compressive modulus for HA20/SF80 chondrocyte-laden constructs was increased almost 10-fold over 28 days of culture in chondrogenic medium which confirmed the superior production of ECM in this hydrogel compared to other hydrogels in this study. Furthermore, in hydrogels loaded with anabolic and anti-inflammatory drugs, HA20/SF80 hydrogel showed the longest and the most sustained release profile over time which is desirable for the long treatment duration typically necessary for osteoarthritic joints. In conclusion, HA20/SF80 hydrogel was successfully established as a suitable injectable biomaterial for cartilage tissue engineering and drug delivery applications.},
  subject      = {Tissue Engineering},
  language  = {en}
}
@article{QuartinelloTallianAueretal.,
  author    = {Quartinello, Felice and Tallian, Claudia and Auer, Julia and Sch{\"o}n, Herta and Vielnascher, Robert and Weinberger, Simone and Wieland, Karin and Weihs, Anna and Rollett, Alexandra and Lendl, Bernhard and Teuschl, Andreas and Pellis, Alessandro and G{\"u}bitz, Georg},
  title     = {Smart Textiles in Wound Care: Functionalization of Cotton/PET Blends with Antimicrobial Nanocapsules},
  series = {Journal of Materials Chemistry B},
  journal   = {Journal of Materials Chemistry B},
  subject      = {Smart textiles},
  language  = {en}
}
@article{FeichtingerMonforteKeibletal.,
  author    = {Feichtinger, Xaver and Monforte, Xavier and Keibl, Claudia and Hercher, David and Schanda, Jakob and Teuschl, Andreas and Muschitz, Christian and Redl, Heinz and Fialka, Christian and Mittermayr, Rainer},
  title     = {Substantial Biomechanical Improvement by Extracorporeal Shockwave Therapy After Surgical Repair of Rodent Chronic Rotator Cuff Tears.},
  series = {American Journal of Sports Medicine},
  journal   = {American Journal of Sports Medicine},
  subject      = {Shockwave Therapy},
  language  = {en}
}
@article{TeuschlTanglHeimeletal.,
  author    = {Teuschl, Andreas and Tangl, Stefan and Heimel, Patrick and Schwarze, Uwe Yacine and Monforte, Xavier and Redl, Heinz and Nau, Thomas},
  title     = {Osteointegration of a Novel Silk Fiber-Based ACL Scaffold by Formation of a Ligament-Bone Interface.},
  series = {American Journal of Sports Medicine},
  journal   = {American Journal of Sports Medicine},
  subject      = {Tissue Engineering},
  language  = {en}
}
@article{SimsaMonforteSalzeretal.,
  author    = {Simsa, Robin and Monforte, Xavier and Salzer, Elias and Teuschl, Andreas and Jenndahl, Lachmi and Bergh, Niklas and Fogelstrand, Per},
  title     = {Effect of fluid dynamics on decellularization efficacy and mechanical properties of blood vessels.},
  series = {PLoS One},
  journal   = {PLoS One},
  subject      = {Tissue Engineering},
  language  = {en}
}
@misc{KueenburgTeuschl,
  author    = {K{\"u}enburg, Bernhard and Teuschl, Andreas},
  title     = {Regenerative silk ligament: scale up and regulatory strategy of a textile engineered silk implant for tissue regeneration of injured human ACL (anterior cruciate ligament)},
  abstract  = {61 a93591 27-SY-1 Regenerative silk ligament: scale up and regulatory strategy of a textile engineered silk implant for tissue regeneration of injured human ACL (anterior cruciate ligament) Bernhard K{\"u}enburg，Andreas Teuschl MorphoMed, Austria In the USA around 370.000 annual ACL ruptures in the predominately young population (age 18-30) following sport injuries cause a lot of pain and long term troubles for the affected patients. The well established standard procedure of autologous tissue transplantation stabilizes the knee and allows sport activities after 9-12 months. However, harvest of autologous tissue such as patellar tendon or semitendinosus (the most frequently used autograft) weakens other body areas and long term data show an increased rate of arthroses (up to 50\% after 10 years) associated with ACL reconstructions. Based on numerous preclinical data and data from a 12-month sheep study the scale-up as well as regulatory approval process of a novel textile engineered silk implant as ACL graft have been initiated. It is planned to enter a clinical trial and strive for regulatory approval as a medical class 3 implant. The sheep data (1) have revealed an approximately 50\% degradation of the purified medical silk, which acts as scaffold for the regrowth of a new endogenous ligament. The formation of oriented collagenous tissue fibers including vascularization proves a ligament tissue regeneration for the first time. Scale-up and implementation of a commercial process require a defined set of specifications of commercial silkworm (Bombyx mori) silk, a biological raw material sourced from the textile industry as well as adequate analytical methods to characterize the depletion of sericin in the course of the production process. Based on first full scale samples, the defined biocompatibility program has to be executed, in the EU following the ISO requirements of the notified bodies, in the USA determined by the FDA. Upon submission of the full technical documentation as well as the biocompati-bility data, approval of a clinical trial can be achieved in order to demonstrate the clinical efficacy of the silk based ACL graft compared to the gold standard method in two different randomized groups. As primary endpoint the knee stability will be tested by an apparatus supported Lachmann test. In this study, besides the clinical benefit the patient safety is an important goal. The results of this study will be used for achievement of regulatory approval. References: 1) Teuschl A, Heimel P, N{\"u}rnberger S, van Griensven M, Redl H, Nau T. Am. J. Sports Med. 2016;44:1547-57. 2) Teuschl AH, van Griensven M, Redl H. Tissue Eng. Part C Methods. 2014;20:431-9.},
  subject      = {Biomaterial},
  language  = {en}
}
@article{TallianHerreroRollettStadleretal.,
  author    = {Tallian, Claudia and Herrero-Rollett, Alexandra and Stadler, Karina and Vielnascher, Robert and Wieland, Karin and Weihs, Anna and Pellis, Alessandro and Teuschl, Andreas and Lendl, Bernhard and Amenitsch, Heinz and Guebitz, Georg M.},
  title     = {Structural insights into pH-responsive drug release of self-assembling human serum albumin-silk fibroin nanocapsules.},
  series = {European Journal of Pharmaceutics and Biopharmaceutics},
  journal   = {European Journal of Pharmaceutics and Biopharmaceutics},
  abstract  = {Inflammation processes are associated with significant decreases in tissue or lysosomal pH from 7.4 to 4, a fact that argues for the application of pH-responsive drug delivery systems. However, for their design and optimization a full understanding of the release mechanism is crucial. In this study we investigated the pH-depending drug release mechanism and the influence of silk fibroin (SF) concentration and SF degradation degree of human serum albumin (HSA)-SF nanocapsules. Sonochemically produced nanocapsules were investigated regarding particle size, colloidal stability, protein encapsulation, thermal stability and drug loading properties. Particles of the monodisperse phase showed average hydrodynamic radii between 438 and 888 nm as measured by DLS and AFM and a zeta potential of -11.12 ± 3.27 mV. Together with DSC results this indicated the successful production of stable nanocapsules. ATR-FTIR analysis demonstrated that SF had a positive effect on particle formation and stability due to induced beta-sheet formation and enhanced crosslinking. The pH-responsive release was found to depend on the SF concentration. In in-vitro release studies, HSA-SF nanocapsules composed of 50\% SF showed an increased pH-responsive release for all tested model substances (Rhodamine B, Crystal Violet and Evans Blue) and methotrexate at the lowered pH of 4.5 to pH 5.4, while HSA capsules without SF did not show any pH-responsive drug release. Mechanistic studies using confocal laser scanning microscopy (CLSM) and small angle X-ray scattering (SAXS) analyses showed that increases in particle porosity and decreases in particle densities are directly linked to pH-responsive release properties. Therefore, the pH-responsive release mechanism was identified as diffusion controlled in a novel and unique approach by linking scattering results with in vitro studies. Finally, cytotoxicity studies using the human monocytic THP-1 cell line indicated non-toxic behavior of the drug loaded nanocapsules when applied in a concentration of 62.5 µg mL-1.},
  subject      = {Biomaterial},
  language  = {en}
}
@misc{TeuschlSchuhWeihsetal.,
  author    = {Teuschl, Andreas and Schuh, Christina and Weihs, Anna and Guillaume, Olivier and Monforte Vila, Xavier and Redl, Heinz and Kaplan, David and R{\"u}nzler, Dominik},
  title     = {Tailoring bioactivity of silk-based biomaterials via delivering and functionalization strategies with fibrinogen/thrombin, plant lectins or laminin},
  subject      = {Biomaterials},
  language  = {en}
}
@article{SlezakSlezakHartingeretal.,
  author    = {Slezak, Paul and Slezak, Cyrill and Hartinger, Joachim and Teuschl, Andreas and N{\"u}rnberger, Sylvia and Redl, Heinz and Mittermayr, Rainer},
  title     = {A Low Cost Implantation Model in the Rat That Allows a Spatial Assessment of Angiogenesis.},
  series = {Frontiers in Bioengineering and Biotechnology},
  journal   = {Frontiers in Bioengineering and Biotechnology},
  abstract  = {There is continual demand for animal models that allow a quantitative assessment of angiogenic properties of biomaterials, therapies, and pharmaceuticals. In its simplest form, this is done by subcutaneous material implantation and subsequent vessel counting which usually omits spatial data. We have refined an implantation model and paired it with a computational analytic routine which outputs not only vessel count but also vessel density, distribution, and vessel penetration depth, that relies on a centric vessel as a reference point. We have successfully validated our model by characterizing the angiogenic potential of a fibrin matrix in conjunction with recombinant human vascular endothelial growth factor (rhVEGF165). The inferior epigastric vascular pedicles of rats were sheathed with silicone tubes, which were subsequently filled with 0.2 ml of fibrin and different doses of rhVEGF165, centrically embedding the vessels. Over 4 weeks, tissue samples were harvested and subsequently immunohistologically stained and computationally analyzed. The model was able to detect variations over the angiogenic potentials of growth factor spiked fibrin matrices. Adding 20 ng of rhVEGF165 resulted in a significant increase in vasculature while 200 ng of rhVEGF165 did not improve vascular growth. Vascularized tissue volume increased during the first week and vascular density increased during the second week. Total vessel count increased significantly and exhibited a peak after 2 weeks which was followed by a resorption of vasculature by week 4. In summary, a simple implantation model to study in vivo vascularization with only a minimal workload attached was enhanced to include morphologic data of the emerging vascular tree.},
  subject      = {Tissue Engineering},
  language  = {en}
}
@article{BerkovitchCohenPeledetal.,
  author    = {Berkovitch, Yulia and Cohen, Talia and Peled, Eli and Schmidhammer, Robert and Hildner, Florian and Teuschl, Andreas and Wolbank, Susanne and Yelin, Dvir and Redl, Heinz and Seliktar, Dror},
  title     = {Hydrogel composition and laser micropatterning to regulate sciatic nerve regeneration.},
  series = {Journal of Tissue Engineering and Regenerative Medicine},
  journal   = {Journal of Tissue Engineering and Regenerative Medicine},
  pages     = {1049 -- 1061},
  abstract  = {Treatment of peripheral nerve injuries has evolved over the past several decades to include the use of sophisticated new materials endowed with trophic and topographical cues that are essential for in vivo nerve fibre regeneration. In this research, we explored the use of an advanced design strategy for peripheral nerve repair, using biological and semi-synthetic hydrogels that enable controlled environmental stimuli to regenerate neurons and glial cells in a rat sciatic nerve resection model. The provisional nerve growth conduits were composed of either natural fibrin or adducts of synthetic polyethylene glycol and fibrinogen or gelatin. A photo-patterning technique was further applied to these 3D hydrogel biomaterials, in the form of laser-ablated microchannels, to provide contact guidance for unidirectional growth following sciatic nerve injury. We tested the regeneration capacity of subcritical nerve gap injuries in rats treated with photo-patterned materials and compared these with injuries treated with unpatterned hydrogels, either stiff or compliant. Among the factors tested were shear modulus, biological composition, and micropatterning of the materials. The microchannel guidance patterns, combined with appropriately matched degradation and stiffness properties of the material, proved most essential for the uniform tissue propagation during the nerve regeneration process.},
  subject      = {Tissue Engineering},
  language  = {en}
}
@article{NauTeuschlEbneretal.,
  author    = {Nau, Thomas and Teuschl, Andreas and Ebner, Anna and Jung, Ilse and Schenk, Christian},
  title     = {Low revision rate and excellent outcome of primary ACL repair with a minimum follow-up of 5 years.},
  series = {Muscle, ligaments and tendons Journal},
  journal   = {Muscle, ligaments and tendons Journal},
  pages     = {185 -- 190},
  abstract  = {Introduction: Due to limitations of ACL reconstruction, primary ACL repair has recently regained research interest. Although abandoned in the past, primary repair with conservation of the original ligament demonstrates considerable advantages compared to reconstruction. We hypothesized that early repair, strictly limited to patients with a proximal ACL rupture and excellent tissue quality of the remaining ACL stump, would lead to equal revision rates and subjective outcomes as reported for ACL reconstruction after a minimum of 5 years. Methods: In this questionnaire study, patients who had a primary ACL repair between 2002 and 2009 were invited to participate. Besides any potential revision surgery, the Tegner activity scale and the Knee Injury and Osteoarthritis Outcome Score were included in the evaluation. Results: Out of 1912 patients who had ACL related surgery during the observation period, 221 (11.4\%) had a primary ACL repair. 60 patients (61 knees) were available for follow-up. In 2/61 (3.3\%) cases ACL revision surgery was performed and one patient had meniscus surgery of the affected side. The median Tegner activity scale was 6 (range, 3 to 10). The mean KOOS subscores were 88.8\% (Function/Sports), 86.6\% (Quality of life), 94.6 (Symptoms), 94.0 (Pain) and 97.0 (Activities of Daily Living). Conclusion: Primary ACL repair, strictly limited to proximal ruptures with good tissue quality leads to revision rates and subjective outcome comparable to ACL reconstruction. Level of evidence: IV.},
  subject      = {Regeneration},
  language  = {en}
}
@misc{TeuschlHeimelMonforteVilaetal.,
  author    = {Teuschl, Andreas and Heimel, Patrick and Monforte Vila, Xavier and N{\"u}rnberger, Sylvia and Tangl, Stefan and van Griensven, Martijn and Redl, Heinz and Nau, Thomas},
  title     = {Anterior cruciate ligament regeneration using the silk-based RegACL scaffold},
  subject      = {Tissue Engineering},
  language  = {en}
}
@incollection{HackethalSchuhHoferetal.,
  author    = {Hackethal, Johannes and Schuh, Christina and Hofer, Alexandra and Meixner, Barbara and Hennerbichler, Simone and Redl, Heinz and Teuschl, Andreas},
  title     = {Human Placenta Laminin-111 as a Multifunctional Protein for Tissue Engineering and Regenerative Medicine},
  series = {Advances in Experimental Medicine and Biology},
  booktitle = {Advances in Experimental Medicine and Biology},
  publisher = {Springer},
  publisher      = {Fachhochschule Technikum Wien},
  subject      = {Biomaterial},
  language  = {en}
}
@misc{SalzerRiederMonforteVilaetal.,
  author    = {Salzer, Elias and Rieder, Bernhard and Monforte Vila, Xavier and Weihs, Anna and R{\"u}nzler, Dominik and Teuschl, Andreas},
  title     = {Evaluation of a novel hydrostatic pressure bioreactor on bovine cartilage chips},
  subject      = {Bioreactor},
  language  = {en}
}
@article{HackethalMuehlederHoferetal.,
  author    = {Hackethal, Johannes and M{\"u}hleder, Severin and Hofer, Alexandra and Schneider, Karl Heinrich and Pr{\"u}ller, Johanna and Hennerbichler, Simone and Redl, Heinz and Teuschl, Andreas},
  title     = {An Effective Method of Atelocollagen Type 1/3 Isolation from Human Placenta and Its In Vitro Characterization in Two-Dimensional and Three-Dimensional Cell Culture Applications},
  series = {Tissue Eng Part C Methods},
  volume    = {23},
  journal   = {Tissue Eng Part C Methods},
  number    = {5},
  pages     = {274 -- 285},
  subject      = {Placenta},
  language  = {en}
}
@inproceedings{FuchsWeihsSzwarcetal.,
  author    = {Fuchs, Christiane and Weihs, Anna and Szwarc, Dorota and Mittermayr, Rainer and R{\"u}nzler, Dominik and Teuschl, Andreas},
  title     = {Shock wave treatment of muscle (stem) cells - a new implementation for regeneration},
  series = {Proceedings of the 20th International Congress of the ISMST},
  booktitle = {Proceedings of the 20th International Congress of the ISMST},
  subject      = {Shockwave treatment},
  language  = {en}
}
@article{BerkovitchCohenPeledetal.,
  author    = {Berkovitch, Yulia and Cohen, Talia and Peled, Eli and Schmidhammer, Robert and Hildner, Florian and Teuschl, Andreas and Wolbank, Susanne and Yelin, Dvir and Redl, Heinz and Seliktar, Dror},
  title     = {Hydrogel Composition and Laser Micro-Patterning to Regulate Sciatic Nerve Regeneration},
  series = {Journal of Tissue Engineering and Regenerative Medicine},
  volume    = {12},
  journal   = {Journal of Tissue Engineering and Regenerative Medicine},
  number    = {4},
  subject      = {Micro-Patterning},
  language  = {en}
}
@misc{FuchsWeihsSzwarcetal.,
  author    = {Fuchs, Christiane and Weihs, Anna and Szwarc, Dorota and Mittermayr, Rainer and R{\"u}nzler, Dominik and Teuschl, Andreas},
  title     = {Shock wave treatment of muscle (stem) cells - a new implementation for regeneration},
  subject      = {Shockwave treatment},
  language  = {en}
}
@misc{TeuschlWeihsFuchsetal.,
  author    = {Teuschl, Andreas and Weihs, Anna and Fuchs, Christiane and Monforte Vila, Xavier},
  title     = {Silk as a versatile biomaterial for musculoskeletal tissue engineering},
  subject      = {Silk},
  language  = {en}
}
@article{SchneiderLehmannvanOschetal.,
  author    = {Schneider, Cornelia and Lehmann, Johannes and van Osch, Gerjo and Hildner, Florian and Teuschl, Andreas and Monforte Vila, Xavier and Miosga, David and Heimel, Patrick and Priglinger, Eleni and Redl, Heinz and Wolbank, Susanne and N{\"u}rnberger, Sylvia},
  title     = {Systematic Comparison of Protocols for the Preparation of Human Articular Cartilage for Use as Scaffold Material in Cartilage Tissue Engineering},
  series = {Tissue Eng Part C Methods},
  volume    = {22},
  journal   = {Tissue Eng Part C Methods},
  number    = {12},
  subject      = {Cartilage},
  language  = {en}
}
@inproceedings{MaleinerHeherTeuschletal.,
  author    = {Maleiner, Babette and Heher, Philipp and Teuschl, Andreas and Redl, Heinz and R{\"u}nzler, Dominik and Fuchs, Christiane},
  title     = {Generation of aligned skeletal muscle-like tissue based on the application of strain to a 3D fibrin scaffold},
  series = {Proceedings of PACT "Designer Cells go Clinical" Symposium},
  booktitle = {Proceedings of PACT "Designer Cells go Clinical" Symposium},
  subject      = {Tissue Generation},
  language  = {en}
}
@misc{SzwarcFuchsWeihsetal.,
  author    = {Szwarc, Dorota and Fuchs, Christiane and Weihs, Anna and Monforte Vila, Xavier and Hanetseder, Dominik and Teuschl, Andreas and R{\"u}nzler, Dominik},
  title     = {The effect of shock waves on in vitro cartilage development in silk scaffolds},
  subject      = {Shockwave treatment},
  language  = {en}
}
@misc{MaleinerHeherTeuschletal.,
  author    = {Maleiner, Babette and Heher, Philipp and Teuschl, Andreas and Redl, Heinz and R{\"u}nzler, Dominik and Fuchs, Christiane},
  title     = {Generation of aligned skeletal muscle-like tissue based on the application of strain to a 3D fibrin scaffold},
  subject      = {Tissue Generation},
  language  = {en}
}
@misc{TeuschlFuchsWeihsetal.,
  author    = {Teuschl, Andreas and Fuchs, Christiane and Weihs, Anna and Heimel, Patrick and R{\"u}nzler, Dominik and Redl, Heinz and Nau, Thomas},
  title     = {The Silk Road from Textiles to Novel Medical Implants},
  subject      = {Silk},
  language  = {en}
}
@misc{TeuschlHeimelNuernbergeretal.,
  author    = {Teuschl, Andreas and Heimel, Patrick and N{\"u}rnberger, Sylvia and Redl, Heinz and Nau, Thomas},
  title     = {ACL Regeneration using a novel silk fiber based scaffold - Histological Results of a Large Animal Study},
  subject      = {Tissue Regeneration},
  language  = {en}
}
@misc{Teuschl,
  author    = {Teuschl, Andreas},
  title     = {Silk Processing - from Gels to ACL Replacement},
  subject      = {Silk},
  language  = {en}
}
@article{HeherMaleinerPruelleretal.,
  author    = {Heher, Philipp and Maleiner, Babette and Pr{\"u}ller, Johanna and Teuschl, Andreas and Kollmitzer, Josef and Monforte Vila, Xavier and Wolbank, Susanne and Redl, Heinz and R{\"u}nzler, Dominik and Fuchs, Christiane},
  title     = {A novel bioreactor for the generation of highly aligned 3D skeletal muscle-like constructs through orientation of fibrin via application of static strain},
  series = {Acta Biomaterialia},
  journal   = {Acta Biomaterialia},
  subject      = {Bioreactor},
  language  = {en}
}
@article{TeuschlSchuhHalbweisetal.,
  author    = {Teuschl, Andreas and Schuh, Christina and Halbweis, Robert and Pajer, Krisztian and Marton, Gabor and Hopf, Rudolf and Mosia, Shorena and R{\"u}nzler, Dominik and Redl, Heinz and Nogradi, Antal and Hausner, Thomas},
  title     = {A new preparation method for anisotropic silk fibroin nerve guidance conduits and its evaluation in vitro and in a rat sciatic nerve defect model},
  series = {Tissue Engineering Part C: Methods},
  journal   = {Tissue Engineering Part C: Methods},
  subject      = {Fibrin},
  language  = {en}
}
@misc{TeuschlSchuhHalbweisetal.,
  author    = {Teuschl, Andreas and Schuh, Christina and Halbweis, Robert and Marton, G{\´a}bor and Pajer, Kriszti{\´a}n and Hopf, Rudolf and Mosia, Shorena and R{\"u}nzler, Dominik and N{\´o}gr{\´a}di, Antal and Hausner, Thomas and Redl, Heinz},
  title     = {Silk fibroin for peripheral nerve regeneration: a novel preparation method improved mechanical characteristics and supports regeneration in rat sciatic nerves},
  subject      = {Fibroin},
  language  = {en}
}
@misc{FuchsWeihsTeuschletal.,
  author    = {Fuchs, Christiane and Weihs, Anna and Teuschl, Andreas and Hartinger, Joachim and Slezak, Paul and Mittermayr, Rainer and Redl, Heinz and Junger, Wolfgang and Sitte, Harald and R{\"u}nzler, Dominik},
  title     = {Shockwave Treatment Enhances Proliferation and Improves Wound Healing via Purinergic Signaling Linked ERK 1/2 Pathways},
  subject      = {Shockwave treatment},
  language  = {en}
}
@misc{FuchsWeihsTeuschletal.,
  author    = {Fuchs, Christiane and Weihs, Anna and Teuschl, Andreas and Hartinger, Joachim and Slezak, Paul and Mittermayr, Rainer and Redl, Heinz and Junger, Wolfgang and Sitte, Harald and R{\"u}nzler, Dominik},
  title     = {Shockwave Treatment Augments Proliferation and Improves Wound Healing via Purinergic Signaling Linked ERK 1/2 Pathways},
  subject      = {Shockwave treatment},
  language  = {en}
}
@article{GuillaumeParkMonforteVilaetal.,
  author    = {Guillaume, Olivier and Park, Jaesung and Monforte Vila, Xavier and Gruber-Blum, Simone and Redl, Heinz and Petter-Puchner, Akexander and Teuschl, Andreas},
  title     = {Fabrication of silk mesh with enhanced cytocompatibility: preliminary in vitro investigation toward cell-based therapy for hernia repair},
  series = {Journal of Materials Science: Materials in Medicine},
  journal   = {Journal of Materials Science: Materials in Medicine},
  subject      = {Cytocompatibility},
  language  = {en}
}
@misc{WeihsFuchsTeuschletal.,
  author    = {Weihs, Anna and Fuchs, Christiane and Teuschl, Andreas and Hartinger, Joachim and Slezak, Paul and Mittermayr, Rainer and Redl, Heinz and Junger, Wolfgang and Sitte, Harald and R{\"u}nzler, Dominik},
  title     = {Shockwave treatment activates Erk1/2 pathways predominantly via P2Y receptor involvement},
  subject      = {Shockwave},
  language  = {en}
}
@article{TeuschlHeimelNuernbergeretal.,
  author    = {Teuschl, Andreas and Heimel, Patrick and N{\"u}rnberger, Sylvia and van Griensven, Martijn and Redl, Heinz and Nau, Thomas},
  title     = {A Novel Silk Fiber-Based Scaffold for Regeneration of the Anterior Cruciate Ligament: Histological Results From a Study in Sheep.},
  series = {The American Journal of Sports Medicine},
  journal   = {The American Journal of Sports Medicine},
  subject      = {Ligament},
  language  = {en}
}
@misc{TeuschlFuchsFeichtingeretal.,
  author    = {Teuschl, Andreas and Fuchs, Christiane and Feichtinger, Georg and Heher, Philipp and Heimel, Patrick and Schuh, Christina and N{\"u}rnberger, Sylvia and Nau, Thomas and R{\"u}nzler, Dominik and Redl, Heinz},
  title     = {Fibrin or Fibroin - Not only the "o" Makes the Difference},
  subject      = {Fibrin},
  language  = {en}
}
@article{TeuschlBalmayorRedletal.,
  author    = {Teuschl, Andreas and Balmayor, Elizabeth and Redl, Heinz and van Griensven, Martijn and Dungel, Peter},
  title     = {Phototherapy With LED Light Modulates Healing Processes in an In Vitro Scratch Wound-Model Using 3 Different Cell Types},
  series = {Dermatologic Surgery},
  volume    = {41},
  journal   = {Dermatologic Surgery},
  number    = {2},
  pages     = {261 -- 268},
  subject      = {Phototherapy},
  language  = {en}
}
@article{SchuhHercherStaineretal.,
  author    = {Schuh, Christina and Hercher, David and Stainer, Michaela and Hopf, Rudolf and Teuschl, Andreas and Schmidhammer, Robert and Redl, Heinz},
  title     = {Extracorporeal shockwave treatment: A novel tool to improve Schwann cell isolation and culture},
  series = {Cytotherapy},
  journal   = {Cytotherapy},
  subject      = {Extracorporeal shockwave},
  language  = {en}
}
@misc{TeuschlNuernbergerHeimeletal.,
  author    = {Teuschl, Andreas and N{\"u}rnberger, Sylvia and Heimel, Patrick and Redl, Heinz and Nau, Thomas},
  title     = {Regeneration of the Anterior Cruciate Ligament Using a Silk-Fiber Based Scaffold - Histological Results},
  subject      = {Tissue Regeneration},
  language  = {en}
}
@article{NauRedlTeuschl,
  author    = {Nau, Thomas and Redl, Heinz and Teuschl, Andreas},
  title     = {Comment on: In Vivo Evaluation of Electrospun Polycaprolactone Graft for Anterior Cruciate Ligament Engineering},
  series = {Tissue Engineering Part A},
  journal   = {Tissue Engineering Part A},
  subject      = {Grafting},
  language  = {en}
}
@article{GauillaumeTeuschlGruberBlum,
  author    = {Gauillaume, Oliver and Teuschl, Andreas and Gruber-Blum, Simone},
  title     = {Emerging Trends in Abdominal Wall Reinforcement: Bringing Bio-Functionality to Meshes},
  series = {Advanced Healthcare Materials},
  journal   = {Advanced Healthcare Materials},
  subject      = {Abdominal Wall Reinforcement},
  language  = {en}
}
@article{NauTeuschl,
  author    = {Nau, Thomas and Teuschl, Andreas},
  title     = {Regeneration of the anterior cruciate ligament: Current strategies in tissue engineering},
  series = {World Journal of Orthopedics},
  volume    = {6},
  journal   = {World Journal of Orthopedics},
  number    = {1},
  subject      = {Ligament},
  language  = {en}
}
@article{WeihsFuchsTeuschletal.,
  author    = {Weihs, Anna and Fuchs, Christiane and Teuschl, Andreas and Hartinger, Joachim and Slezak, Paul and Mittermayr, Rainer and Redl, Heinz and Junger, Wolfgang and Sitte, Harald and R{\"u}nzler, Dominik},
  title     = {Shock Wave Treatment Enhances Cell Proliferation and Improves Wound Healing by ATP Release-coupled Extracellular Signal-regulated Kinase (ERK) Activation},
  series = {The Journal of biological chemistry},
  journal   = {The Journal of biological chemistry},
  subject      = {Shockwave},
  language  = {de}
}
@article{SchuhHeherWeihsetal.,
  author    = {Schuh, Christina and Heher, Philipp and Weihs, Anna and Asmita, Banerjee and Wolbank, Susanne and Mittermayr, Rainer and Redl, Heinz and R{\"u}nzler, Dominik and Teuschl, Andreas},
  title     = {Adipose derived stem cells respond to in vitro extracorporeal shockwave treatment with increased stemness and multipotency},
  series = {New Biotechnology},
  journal   = {New Biotechnology},
  subject      = {Shockwave},
  language  = {en}
}
@misc{HeherFuchsPruelleretal.,
  author    = {Heher, Philipp and Fuchs, Christiane and Pr{\"u}ller, Johanna and Maleiner, Babette and Kollmitzer, Josef and R{\"u}nzler, Dominik and Teuschl, Andreas and Wolbank, Susanne and Redl, Heinz},
  title     = {A bioreactor-based 3D culture system for skeletal muscle engineering in fibrin scaffolds},
  subject      = {Bioreactors},
  language  = {en}
}
@article{BanerjeeNuernbergerHennerbichleretal.,
  author    = {Banerjee, Asmita and N{\"u}rnberger, Sylvia and Hennerbichler, Simone and Riedl, Stefan and Schuh, Christina and Hacobian, Ara and Teuschl, Andreas and Eibl, J{\"u}rgen and Redl, Heinz},
  title     = {In toto differentiation of human amniotic membrane towards the Schwann cell lineage},
  series = {227-239},
  volume    = {15},
  journal   = {227-239},
  number    = {2},
  subject      = {Membrane},
  language  = {en}
}
@article{TeuschlNeutschMonforteVilaetal.,
  author    = {Teuschl, Andreas and Neutsch, Lukas and Monforte Vila, Xavier and R{\"u}nzler, Dominik and van Griensven, Martijn and Gabor, Franz and Redl, Heinz},
  title     = {Enhanced cell adhesion on silk fibroin via lectin surface modification.},
  series = {Acta Biomaterialia},
  journal   = {Acta Biomaterialia},
  subject      = {Silk},
  language  = {en}
}
@article{HohlriederTeuschlCichaetal.,
  author    = {Hohlrieder, Manfred and Teuschl, Andreas and Cicha, Klaus and van Griensven, Martijn and Redl, Heinz and Stampfl, J{\"u}rgen},
  title     = {Bioreactor and scaffold design for the mechanical stimulation of anterior cruciate ligament grafts},
  series = {Biomedical materials and engineering},
  volume    = {23},
  journal   = {Biomedical materials and engineering},
  number    = {3},
  pages     = {225 -- 237},
  subject      = {Bioreactors},
  language  = {en}
}
@article{DungelTeuschlBanerjeeetal.,
  author    = {Dungel, Peter and Teuschl, Andreas and Banerjee, Asmita and Paier-Pourani, Jamile and Redl, Heinz and Kozlov, Andrey},
  title     = {Impact of mitochondria on nitrite metabolism in HL-1 cardiomyocytes},
  series = {Frontiers in Physiology},
  journal   = {Frontiers in Physiology},
  number    = {4},
  subject      = {Nitrite},
  language  = {en}
}
@article{TeuschlNuernbergerRedletal.,
  author    = {Teuschl, Andreas and N{\"u}rnberger, Sylvia and Redl, Heinz and Nau, Thomas},
  title     = {Articular cartilage tissue regeneration: current research strategies and outlook for the future},
  series = {European Surgery},
  volume    = {45},
  journal   = {European Surgery},
  number    = {3},
  pages     = {142 -- 153},
  subject      = {Tissue Regeneration},
  language  = {en}
}
@article{TeuschlvanGriensvenRedl,
  author    = {Teuschl, Andreas and van Griensven, Martijn and Redl, Heinz},
  title     = {Sericin removal from raw Bombys mori silk scaffolds of high hierarchical order},
  series = {Tissue Eng Part C Methods},
  journal   = {Tissue Eng Part C Methods},
  subject      = {Scaffold},
  language  = {en}
}
@article{RiederWeihsTeuschletal.,
  author    = {Rieder, Bernhard and Weihs, Anna and Teuschl, Andreas and Knebl, Gerald and Kollmitzer, Josef and Redl, Heinz and R{\"u}nzler, Dominik},
  title     = {Evaluation of cell response on permanent and pulsed atmospheric pressure stressed cells},
  series = {Journal of Tissue Engineering and Regenerative Medicine},
  volume    = {1},
  journal   = {Journal of Tissue Engineering and Regenerative Medicine},
  number    = {6},
  pages     = {240 -- 240},
  subject      = {Cells},
  language  = {en}
}
@article{TeuschlAignerHohlriederetal.,
  author    = {Teuschl, Andreas and Aigner, Elmar and Hohlrieder, Martin and Cicha, Klaus and Stampfl, J{\"u}rgen and Redl, Heinz},
  title     = {Stimulation of ligament tissue formation on a silk scaffold with mechanical loading using a custom-made bioreactor system},
  series = {Journal of Tissue Engineering and Regenerative Medicine},
  volume    = {1},
  journal   = {Journal of Tissue Engineering and Regenerative Medicine},
  number    = {6},
  pages     = {51 -- 51},
  subject      = {Ligament},
  language  = {en}
}
@article{TeuschlFergusonSzomolanyietal.,
  author    = {Teuschl, Andreas and Ferguson, James and Szomolanyi, Pavol and Trattnig, Siegfried and Redl, Heinz and Nau, Thomas},
  title     = {Osteointegration of anterior cruciate ligament scaffolds fabricated of bombyx mori silk},
  series = {Journal of Tissue Engineering and Regenerative Medicine},
  volume    = {1},
  journal   = {Journal of Tissue Engineering and Regenerative Medicine},
  number    = {6},
  pages     = {181 -- 182},
  subject      = {Osteointegration},
  language  = {en}
}
@misc{HromadaTomaschWeihsetal.,
  author    = {Hromada, Carina and Tomasch, Janine and Weihs, Anna and R{\"u}nzler, Dominik and Teuschl, Andreas},
  title     = {Engineering of 3D Tissue Constructs Using our Novel MagneTissue Bioreactor as Alternatives to Animal Models},
  subject      = {Bioreactor},
  language  = {en}
}
@article{RiederWeihsWeidingeretal.,
  author    = {Rieder, Bernhard and Weihs, Anna and Weidinger, Adelheid and Sczwarc, Dorota and N{\"u}rnberger, Sylvia and Redl, Heinz and R{\"u}nzler, Dominik and Huber-Gries, Carina and Teuschl, Andreas},
  title     = {Hydrostatic pressure-generated reactive oxygen species induce osteoarthritic conditions in cartilage pellet cultures},
  series = {Scientific Reports},
  journal   = {Scientific Reports},
  subject      = {Bioreactor},
  language  = {en}
}
@article{TeuschlZipperleHuberGriesetal.,
  author    = {Teuschl, Andreas and Zipperle, Johannes and Huber-Gries, Carina and Kaplan, David},
  title     = {Silk fibroin based carrier system for delivery of fibrinogen and thrombin as coagulant supplements},
  series = {Journal of Biomedical Materials Research},
  volume    = {105},
  journal   = {Journal of Biomedical Materials Research},
  number    = {3},
  subject      = {Fibrin},
  language  = {en}
}
@article{SimsaPadmaHeheretal.,
  author    = {Simsa, Robin and Padma, Arvind and Heher, Philipp and Hellstr{\"o}m, Mats and Teuschl, Andreas and Jenndahl, Lachmi and Bergh, Niklas and Fogelstrand, Per},
  title     = {Systematic in vitro comparison of decellularization protocols for blood vessels.},
  series = {PLoS One},
  journal   = {PLoS One},
  subject      = {Tissue Engineering},
  language  = {en}
}
@article{SchneiderEnayatiGrasletal.,
  author    = {Schneider, Karl Heinrich and Enayati, Marjan and Grasl, Christian and Walter, Ingrid and Budinsky, Lubos and Zebic, Gabriel and Kaun, Christoph and Wagner, Anja and Kratochwill, Klaus and Redl, Heinz and Teuschl, Andreas and Podesser, Bruno K. and Bergmeister, Helga},
  title     = {Acellular vascular matrix grafts from human placenta chorion: Impact of ECM preservation on graft characteristics, protein composition and in vivo performance.},
  series = {Biomaterials},
  journal   = {Biomaterials},
  pages     = {14 -- 26},
  abstract  = {Small diameter vascular grafts from human placenta, decellularized with either Triton X-100 (Triton) or SDS and crosslinked with heparin were constructed and characterized. Graft biochemical properties, residual DNA, and protein composition were evaluated to compare the effect of the two detergents on graft matrix composition and structural alterations. Biocompatibility was tested in vitro by culturing the grafts with primary human macrophages and in vivo by subcutaneous implantation of graft conduits (n = 7 per group) into the flanks of nude rats. Subsequently, graft performance was evaluated using an aortic implantation model in Sprague Dawley rats (one month, n = 14). In situ graft imaging was performed using MRI angiography. Retrieved specimens were analyzed by electromyography, scanning electron microscopy, histology and immunohistochemistry to evaluate cell migration and the degree of functional tissue remodeling. Both decellularization methods resulted in grafts of excellent biocompatibility in vitro and in vivo, with low immunogenic potential. Proteomic data revealed removal of cytoplasmic proteins with relative enrichment of ECM proteins in decelluarized specimens of both groups. Noteworthy, LC-Mass Spectrometry analysis revealed that 16 proteins were exclusively preserved in Triton decellularized specimens in comparison to SDS-treated specimens. Aortic grafts showed high patency rates, no signs of thrombus formation, aneurysms or rupture. Conduits of both groups revealed tissue-specific cell migration indicative of functional remodeling. This study strongly suggests that decellularized allogenic grafts from the human placenta have the potential to be used as vascular replacement materials. Both detergents produced grafts with low residual immunogenicity and appropriate mechanical properties. Observed differences in graft characteristics due to preservation method had no impact on successful in vivo performance in the rodent model.},
  subject      = {Biomaterial},
  language  = {en}
}
@article{TeuschlHolnthonerMonforte,
  author    = {Teuschl, Andreas and Holnthoner, Wolfgang and Monforte, Xavier},
  title     = {Repopulation of an auricular cartilage scaffold, AuriScaff, perforated with an enzyme combination},
  series = {Acta Biomater.},
  volume    = {2019},
  journal   = {Acta Biomater.},
  number    = {Mar/86},
  pages     = {207 -- 222},
  abstract  = {Biomaterials currently in use for articular cartilage regeneration do not mimic the composition or architecture of hyaline cartilage, leading to the formation of repair tissue with inferior characteristics. In this study we demonstrate the use of "AuriScaff", an enzymatically perforated bovine auricular cartilage scaffold, as a novel biomaterial for repopulation with regenerative cells and for the formation of high-quality hyaline cartilage. AuriScaff features a traversing channel network, generated by selective depletion of elastic fibers, enabling uniform repopulation with therapeutic cells. The complex collagen type II matrix is left intact, as observed by immunohistochemistry, SEM and TEM. The compressive modulus is diminished, but three times higher than in the clinically used collagen type I/III scaffold that served as control. Seeding tests with human articular chondrocytes (hAC) alone and in co-culture with human adipose-derived stromal/stem cells (ASC) confirmed that the network enabled cell migration throughout the scaffold. It also guides collagen alignment along the channels and, due to the generally traverse channel alignment, newly deposited cartilage matrix corresponds with the orientation of collagen within articular cartilage. In an osteochondral plug model, AuriScaff filled the complete defect with compact collagen type II matrix and enabled chondrogenic differentiation inside the channels. Using adult articular chondrocytes from bovine origin (bAC), filling of even deep defects with high-quality hyaline-like cartilage was achieved after 6 weeks in vivo. With its composition and spatial organization, AuriScaff provides an optimal chondrogenic environment for therapeutic cells to treat cartilage defects and is expected to improve long-term outcome by channel-guided repopulation followed by matrix deposition and alignment. STATEMENT OF SIGNIFICANCE: After two decades of tissue engineering for cartilage regeneration, there is still no optimal strategy available to overcome problems such as inconsistent clinical outcome, early and late graft failures. Especially large defects are dependent on biomaterials and their scaffolding, guiding and protective function. Considering the currently used biomaterials, structure and mechanical properties appear to be insufficient to fulfill this task. The novel scaffold developed within this study is the first approach enabling the use of dense cartilage matrix, repopulate it via channels and provide the cells with a compact collagen type II environment. Due to its density, it also provides better mechanical properties than materials currently used in clinics. We therefore think, that the auricular cartilage scaffold (AuriScaff) has a high potential to improve future cartilage regeneration approaches.},
  subject      = {Auricular cartilage},
  language  = {en}
}
@misc{SchneiderAignerMonforteVilaetal.,
  author    = {Schneider, Karl Heinrich and Aigner, Petra and Monforte Vila, Xavier and Holnthoner, Wolfgang and Teuschl, Andreas and Bergmeister, Helga and Redl, Heinz},
  title     = {Naturally derived acellular small diameter vascular grafts from human placenta for reconstructive surgery},
  subject      = {Placenta},
  language  = {en}
}
@article{SchneiderRohringerKapelleretal.,
  author    = {Schneider, Karl and Rohringer, Sabrina and Kapeller, Barbara and Grasl, Christian and Kiss, Herbert and Heber, Stefan and Walter, Ingrid and Teuschl, Andreas and Podesser, Bruno K. and Bergmeister, Helga},
  title     = {Riboflavin-mediated photooxidation to improve the characteristics of decellularized human arterial small diameter vascular grafts},
  series = {Acta Biomater.},
  volume    = {2020},
  journal   = {Acta Biomater.},
  number    = {116},
  pages     = {246 -- 258},
  abstract  = {Vascular grafts with a diameter of less than 6 mm are made from a variety of materials and techniques to provide alternatives to autologous vascular grafts. Decellularized materials have been proposed as a possible approach to create extracellular matrix (ECM) vascular prostheses as they are naturally derived and inherently support various cell functions. However, these desirable graft characteristics may be limited by alterations of the ECM during the decellularization process leading to decreased biomechanical properties and hemocompatibility. In this study, arteries from the human placenta chorion were decellularized using two distinct detergents (Triton X-100 or SDS), which differently affect ECM ultrastructure. To overcome biomechanical strength loss and collagen fiber exposure after decellularization, riboflavin-mediated UV (RUV) crosslinking was used to uniformly crosslink the collagenous ECM of the grafts. Graft characteristics and biocompatibility with and without RUV crosslinking were studied in vitro and in vivo. RUV-crosslinked ECM grafts showed significantly improved mechanical strength and smoothening of the luminal graft surfaces. Cell seeding using human endothelial cells revealed no cytotoxic effects of the RUV treatment. Short-term aortic implants in rats showed cell migration and differentiation of host cells. Functional graft remodeling was evident in all grafts. Thus, RUV crosslinking is a preferable tool to improve graft characteristics of decellularized matrix conduits.},
  subject      = {Tissue Engineering},
  language  = {en}
}
@article{NuernbergerSchneidervanOschetal.,
  author    = {N{\"u}rnberger, Sylvia and Schneider, Cornelia and van Osch, Gerjo and Keibl, Claudia and Rieder, Bernhard and Monforte, Xavier and Teuschl, Andreas and M{\"u}hleder, Severin and Holnthoner, Wolfgang and Sch{\"a}dl, Barbara and Gahleitner, Christoph and Redl, Heinz and Wolbank, Susanne},
  title     = {Repopulation of an auricular cartilage scaffold, AuriScaff, perforated with an enzyme combination.},
  series = {Acta Biomaterialia},
  journal   = {Acta Biomaterialia},
  subject      = {Tissue Engineering},
  language  = {en}
}
@article{SchneiderRohringerKapelleretal.,
  author    = {Schneider, Karl H. and Rohringer, Sabrina and Kapeller, Barbara and Grasl, Christian and Kiss, Herbert and Heber, Stefan and Walter, Ingrid and Teuschl, Andreas H. and Podesser, Bruno K. and Bergmeister, Helga},
  title     = {Riboflavin-mediated photooxidation to improve the characteristics of decellularized human arterial small diameter vascular grafts},
  series = {Acta Biomaterialia},
  volume    = {116},
  journal   = {Acta Biomaterialia},
  pages     = {246 -- 258},
  abstract  = {Vascular grafts with a diameter of less than 6 mm are made from a variety of materials and techniques to provide alternatives to autologous vascular grafts. Decellularized materials have been proposed as a possible approach to create extracellular matrix (ECM) vascular prostheses as they are naturally derived and inherently support various cell functions. However, these desirable graft characteristics may be limited by alterations of the ECM during the decellularization process leading to decreased biomechanical properties and hemocompatibility. In this study, arteries from the human placenta chorion were decellularized using two distinct detergents (Triton X-100 or SDS), which differently affect ECM ultrastructure. To overcome biomechanical strength loss and collagen fiber exposure after decellularization, riboflavin-mediated UV (RUV) crosslinking was used to uniformly crosslink the collagenous ECM of the grafts. Graft characteristics and biocompatibility with and without RUV crosslinking were studied in vitro and in vivo. RUV-crosslinked ECM grafts showed significantly improved mechanical strength and smoothening of the luminal graft surfaces. Cell seeding using human endothelial cells revealed no cytotoxic effects of the RUV treatment. Short-term aortic implants in rats showed cell migration and differentiation of host cells. Functional graft remodeling was evident in all grafts. Thus, RUV crosslinking is a preferable tool to improve graft characteristics of decellularized matrix conduits.},
  subject      = {Tissue Engineering},
  language  = {en}
}
@article{SchneiderAignerHolnthoneretal.,
  author    = {Schneider, Karl Heinrich and Aigner, Petra and Holnthoner, Wolfgang and Monforte Vila, Xavier and N{\"u}rnberger, Sylvia and R{\"u}nzler, Dominik and Redl, Heinz and Teuschl, Andreas},
  title     = {Decellularized human placenta chorion matrix as a favorable source of small-diameter vascular grafts},
  series = {Acta Biomaterialia},
  journal   = {Acta Biomaterialia},
  subject      = {Grafting},
  language  = {en}
}
@article{HeimelSwiadekSlezaketal.,
  author    = {Heimel, Patrick and Swiadek, Nicole V. and Slezak, Paul and Kerbl, Markus and Schneider, Cornelia and N{\"u}rnberger, Sylvia and Redl, Heinz and Teuschl, Andreas and Hercher, David},
  title     = {Iodine-Enhanced Micro-CT Imaging of Soft Tissue on the Example of Peripheral Nerve Regeneration},
  series = {Contrast Media \& Molecular Imaging},
  journal   = {Contrast Media \& Molecular Imaging},
  subject      = {µCT},
  language  = {en}
}