@article{SchuhHeherWeihsetal.,
  author    = {Schuh, Christina and Heher, Philipp and Weihs, Anna and Fuchs, Christiane and Gabriel, Christian and Wolbank, Susanne and Mittermayr, Rainer and Redl, Heinz and R{\"u}nzler, Dominik and Teuschl, Andreas},
  title     = {In vitro extracorporeal shock wave treatment enhances stemness and preserves multipotency of rat and human adipose-derived stem cells},
  series = {Journal of Cytotherapy},
  journal   = {Journal of Cytotherapy},
  subject      = {Shockwave},
  language  = {en}
}
@article{SimboeckMarksteinerMachaceketal.,
  author    = {Simb{\"o}ck, Elisabeth and Marksteiner, Jessica and Machacek, Thomas and Wiessner, Katharina and Gepp, Barbara and Jesenberger, Veronika and Weihs, Anna and Leitner, Rita},
  title     = {The Power of Problem Based Learning beyond its Didactic Attributes},
  series = {Journal of Problem Based Learning in Higher Education (JPBLHE)},
  volume    = {9},
  journal   = {Journal of Problem Based Learning in Higher Education (JPBLHE)},
  number    = {1},
  pages     = {109 -- 130},
  abstract  = {Hybrid courses with a focus on practice-orientated education and self-guided learning phases are on the rise on the higher education sector. Disciplines in Life Sciences implicate a high degree of practical laboratory expertise. The University of Applied Sciences (UAS) in Vienna, Austria, has thus been endeavoured offering students a high qualitative education integrating hybrid courses based on PBL principles, which consist of on-site (including the transmission of necessary background and practical laboratory training) and off-site (including self-study phases) sessions. As practical laboratory units are central in those courses, the restrictive measures, including the transition to a complete online teaching format due to the first Covid-19-pandemic lock-down, had severe effects on the implementation and the quality of the curriculum. According to surveys made specifically to address this problematic situation, it can be concluded that on-site practical units are fundamental for certain disciplines such as Life Sciences.},
  subject      = {Problem-based Learning},
  language  = {en}
}
@article{QuartinelloTallianAueretal.,
  author    = {Quartinello, Felice and Tallian, Claudia and Auer, Julia and Sch{\"o}n, Herta and Vielnascher, Robert and Weinberger, Simone and Wieland, Karin and Weihs, Anna and Rollett, Alexandra and Lendl, Bernhard and Teuschl, Andreas and Pellis, Alessandro and G{\"u}bitz, Georg},
  title     = {Smart Textiles in Wound Care: Functionalization of Cotton/PET Blends with Antimicrobial Nanocapsules},
  series = {Journal of Materials Chemistry B},
  journal   = {Journal of Materials Chemistry B},
  subject      = {Smart textiles},
  language  = {en}
}
@article{TallianHerreroRollettStadleretal.,
  author    = {Tallian, Claudia and Herrero-Rollett, Alexandra and Stadler, Karina and Vielnascher, Robert and Wieland, Karin and Weihs, Anna and Pellis, Alessandro and Teuschl, Andreas and Lendl, Bernhard and Amenitsch, Heinz and Guebitz, Georg M.},
  title     = {Structural insights into pH-responsive drug release of self-assembling human serum albumin-silk fibroin nanocapsules.},
  series = {European Journal of Pharmaceutics and Biopharmaceutics},
  journal   = {European Journal of Pharmaceutics and Biopharmaceutics},
  abstract  = {Inflammation processes are associated with significant decreases in tissue or lysosomal pH from 7.4 to 4, a fact that argues for the application of pH-responsive drug delivery systems. However, for their design and optimization a full understanding of the release mechanism is crucial. In this study we investigated the pH-depending drug release mechanism and the influence of silk fibroin (SF) concentration and SF degradation degree of human serum albumin (HSA)-SF nanocapsules. Sonochemically produced nanocapsules were investigated regarding particle size, colloidal stability, protein encapsulation, thermal stability and drug loading properties. Particles of the monodisperse phase showed average hydrodynamic radii between 438 and 888 nm as measured by DLS and AFM and a zeta potential of -11.12 ± 3.27 mV. Together with DSC results this indicated the successful production of stable nanocapsules. ATR-FTIR analysis demonstrated that SF had a positive effect on particle formation and stability due to induced beta-sheet formation and enhanced crosslinking. The pH-responsive release was found to depend on the SF concentration. In in-vitro release studies, HSA-SF nanocapsules composed of 50\% SF showed an increased pH-responsive release for all tested model substances (Rhodamine B, Crystal Violet and Evans Blue) and methotrexate at the lowered pH of 4.5 to pH 5.4, while HSA capsules without SF did not show any pH-responsive drug release. Mechanistic studies using confocal laser scanning microscopy (CLSM) and small angle X-ray scattering (SAXS) analyses showed that increases in particle porosity and decreases in particle densities are directly linked to pH-responsive release properties. Therefore, the pH-responsive release mechanism was identified as diffusion controlled in a novel and unique approach by linking scattering results with in vitro studies. Finally, cytotoxicity studies using the human monocytic THP-1 cell line indicated non-toxic behavior of the drug loaded nanocapsules when applied in a concentration of 62.5 µg mL-1.},
  subject      = {Biomaterial},
  language  = {en}
}
@misc{TeuschlSchuhWeihsetal.,
  author    = {Teuschl, Andreas and Schuh, Christina and Weihs, Anna and Guillaume, Olivier and Monforte Vila, Xavier and Redl, Heinz and Kaplan, David and R{\"u}nzler, Dominik},
  title     = {Tailoring bioactivity of silk-based biomaterials via delivering and functionalization strategies with fibrinogen/thrombin, plant lectins or laminin},
  subject      = {Biomaterials},
  language  = {en}
}
@misc{SalzerRiederMonforteVilaetal.,
  author    = {Salzer, Elias and Rieder, Bernhard and Monforte Vila, Xavier and Weihs, Anna and R{\"u}nzler, Dominik and Teuschl, Andreas},
  title     = {Evaluation of a novel hydrostatic pressure bioreactor on bovine cartilage chips},
  subject      = {Bioreactor},
  language  = {en}
}
@misc{SlezakRoseHercheretal.,
  author    = {Slezak, Paul and Rose, Roland and Hercher, David and Weihs, Anna and Fuchs, Christiane and Redl, Heinz and Mittermayr, Rainer and Slezak, Cyrill},
  title     = {Tracking therapeutic shockwaves and their impact on regeneration},
  subject      = {Shockwave Therapy},
  language  = {en}
}
@inproceedings{FuchsWeihsSzwarcetal.,
  author    = {Fuchs, Christiane and Weihs, Anna and Szwarc, Dorota and Mittermayr, Rainer and R{\"u}nzler, Dominik and Teuschl, Andreas},
  title     = {Shock wave treatment of muscle (stem) cells - a new implementation for regeneration},
  series = {Proceedings of the 20th International Congress of the ISMST},
  booktitle = {Proceedings of the 20th International Congress of the ISMST},
  subject      = {Shockwave treatment},
  language  = {en}
}
@misc{FuchsWeihsSzwarcetal.,
  author    = {Fuchs, Christiane and Weihs, Anna and Szwarc, Dorota and Mittermayr, Rainer and R{\"u}nzler, Dominik and Teuschl, Andreas},
  title     = {Shock wave treatment of muscle (stem) cells - a new implementation for regeneration},
  subject      = {Shockwave treatment},
  language  = {en}
}
@misc{TeuschlWeihsFuchsetal.,
  author    = {Teuschl, Andreas and Weihs, Anna and Fuchs, Christiane and Monforte Vila, Xavier},
  title     = {Silk as a versatile biomaterial for musculoskeletal tissue engineering},
  subject      = {Silk},
  language  = {en}
}
@misc{SzwarcFuchsWeihsetal.,
  author    = {Szwarc, Dorota and Fuchs, Christiane and Weihs, Anna and R{\"u}nzler, Dominik},
  title     = {Molecular mechanisms underlying the potential of shock wave treatment for cardiac therapy},
  subject      = {Shockwave treatment},
  language  = {en}
}
@misc{SzwarcFuchsWeihsetal.,
  author    = {Szwarc, Dorota and Fuchs, Christiane and Weihs, Anna and Monforte Vila, Xavier and Hanetseder, Dominik and Teuschl, Andreas and R{\"u}nzler, Dominik},
  title     = {The effect of shock waves on in vitro cartilage development in silk scaffolds},
  subject      = {Shockwave treatment},
  language  = {en}
}
@misc{TeuschlFuchsWeihsetal.,
  author    = {Teuschl, Andreas and Fuchs, Christiane and Weihs, Anna and Heimel, Patrick and R{\"u}nzler, Dominik and Redl, Heinz and Nau, Thomas},
  title     = {The Silk Road from Textiles to Novel Medical Implants},
  subject      = {Silk},
  language  = {en}
}
@inproceedings{FuchsSzwarcWeihsetal.,
  author    = {Fuchs, Christiane and Szwarc, Dorota and Weihs, Anna and R{\"u}nzler, Dominik},
  title     = {Shock wave treatment of 3D cardiac model systems activates ERK 1/2 signaling pathway and influences cardiomyogenesis},
  series = {Proceedings of PACT "Designer Cells go Clinical" Symposium},
  booktitle = {Proceedings of PACT "Designer Cells go Clinical" Symposium},
  subject      = {Shockwave treatment},
  language  = {en}
}
@inproceedings{FuchsSzwarcWeihsetal.,
  author    = {Fuchs, Christiane and Szwarc, Dorota and Weihs, Anna and R{\"u}nzler, Dominik},
  title     = {Shock wave treatment of 3D cardiac model systems activates ERK 1/2 signaling pathway and influences cardiomyogenesis},
  series = {Proceedings of LBG Meeting for Health Sciences 2016},
  booktitle = {Proceedings of LBG Meeting for Health Sciences 2016},
  subject      = {Shockwave treatment},
  language  = {en}
}
@misc{FuchsSzwarcWeihsetal.,
  author    = {Fuchs, Christiane and Szwarc, Dorota and Weihs, Anna and R{\"u}nzler, Dominik},
  title     = {Shock wave treatment of 3D cardiac model systems activates ERK 1/2 signaling pathway and influences cardiomyogenesis},
  subject      = {Shockwave treatment},
  language  = {en}
}
@inproceedings{FuchsSzwarcWeihsetal.,
  author    = {Fuchs, Christiane and Szwarc, Dorota and Weihs, Anna and R{\"u}nzler, Dominik},
  title     = {Shock wave treatment positively influences cardiomyogenesis in an energy-dependent manner},
  series = {Proceedings of PACT "Designer Cells go Clinical" Symposium},
  booktitle = {Proceedings of PACT "Designer Cells go Clinical" Symposium},
  subject      = {Shockwave treatment},
  language  = {en}
}
@misc{Weihs,
  author    = {Weihs, Anna},
  title     = {Shock wave treatment for in vitro tissue engineering applications},
  subject      = {Shockwave treatment},
  language  = {en}
}
@misc{Weihs,
  author    = {Weihs, Anna},
  title     = {Shock wave treatment for in vitro tissue engineering applications},
  subject      = {Shockwave treatment},
  language  = {en}
}
@misc{Weihs,
  author    = {Weihs, Anna},
  title     = {Mechanotransduction in shock wave treatment},
  subject      = {Shockwave treatment},
  language  = {en}
}
@misc{FuchsWeihsTeuschletal.,
  author    = {Fuchs, Christiane and Weihs, Anna and Teuschl, Andreas and Hartinger, Joachim and Slezak, Paul and Mittermayr, Rainer and Redl, Heinz and Junger, Wolfgang and Sitte, Harald and R{\"u}nzler, Dominik},
  title     = {Shockwave Treatment Enhances Proliferation and Improves Wound Healing via Purinergic Signaling Linked ERK 1/2 Pathways},
  subject      = {Shockwave treatment},
  language  = {en}
}
@misc{FuchsWeihsTeuschletal.,
  author    = {Fuchs, Christiane and Weihs, Anna and Teuschl, Andreas and Hartinger, Joachim and Slezak, Paul and Mittermayr, Rainer and Redl, Heinz and Junger, Wolfgang and Sitte, Harald and R{\"u}nzler, Dominik},
  title     = {Shockwave Treatment Augments Proliferation and Improves Wound Healing via Purinergic Signaling Linked ERK 1/2 Pathways},
  subject      = {Shockwave treatment},
  language  = {en}
}
@misc{WeihsFuchsTeuschletal.,
  author    = {Weihs, Anna and Fuchs, Christiane and Teuschl, Andreas and Hartinger, Joachim and Slezak, Paul and Mittermayr, Rainer and Redl, Heinz and Junger, Wolfgang and Sitte, Harald and R{\"u}nzler, Dominik},
  title     = {Shockwave treatment activates Erk1/2 pathways predominantly via P2Y receptor involvement},
  subject      = {Shockwave},
  language  = {en}
}
@phdthesis{Weihs,
  author    = {Weihs, Anna},
  title     = {Elucidation of extracorporeal shock wave treatment triggered intracellular processes},
  school      = {Fachhochschule Technikum Wien},
  abstract  = {Die Wirkung von Stoßwellen auf den menschlichen K{\"o}rper ist bereits seit Jahrzehnten bekannt. Ihr zerst{\"o}rerisches Potential wird seit den 1980-ern zur Behandlung und Desintegration von Nierensteinen angewendet, wo die Stoßwellentherapie heutzutage die Standardbehandlung darstellt. Der erste unerwartete Effekt der Therapie - die Verdichtung des Beckenkammes - wurde schon damals bei Folgeuntersuchungen von Patienten mit Nierensteinen festgestellt. Die Beobachtung, dass Stoßwellen Effekte auf Knochen hervorrufen, ebnete den Weg f{\"u}r die Anwendung der Stoßwellentherapie in Bereichen abseits der Urologie. Mittlerweile wird die Stoßwellentherapie nicht nur zur Behandlung von Knochenbruchheilungsst{\"o}rungen und Tendinopathien, sondern auch bei Weichteilwundheilungsst{\"o}rungen eingesetzt. Trotz der vielf{\"a}ltigen Anwendungsgebiete ist der zugrunde liegende Wirkungsmechanismus des positiven Effekts der Stoßwellentherapie bis heute noch nicht vollst{\"a}ndig aufgekl{\"a}rt. Die Aktivierung von mechanotransduktiven Signalwegen wurde bereits teilweise in vitro und in vivo gezeigt, jedoch meist bei kn{\"o}chernen Indikationen. Doch auch f{\"u}r die Anwendung der Stoßwellentherapie in der (verz{\"o}gerten) Wundheilung ist die Aufkl{\"a}rung des Wirkungsmechanismus essentiell. Erst dadurch k{\"o}nnte diese nicht- invasive, effiziente und großteils Nebenwirkungs-freie Therapieform auch in diesem Bereich als m{\"o}gliche Standardtherapieform genutzt werden. In dieser Dissertation wurde zuerst ein Set-up zur in vitro Stoßwellenanwendung optimiert. Mithilfe eines Molek{\"u}l-Aufnahme Assays wurden die technischen Parameter f{\"u}r die in vitro Stoßwellenbehandlung festgelegt. Mit diesem in vitro Aufbau sollten daraufhin jene intrazellul{\"a}ren Mechanismen identifiziert werden, die von der Stoßwelle beeinflusst werden. In einem in vivo Modell sollte schlussendlich die Rolle dieser Mechanismen am wundheilungsf{\"o}rdernden Effekt der Stoßwelle gekl{\"a}rt werden. Um m{\"o}glichst universelle Effekte der Stoßwelle auf intrazellul{\"a}re Signalwege zu untersuchen, wurden verschiedenste Zelllinien verwendet. Diese beinhalteten die humane U937 Monozyten Zelllinie, humane Jurkat T-Zellen, die humane MG63 Osteosarcoma Zelllinie, die murine C3H10T1/2 mesenchymale Progenitor Zelllinie sowie prim{\"a}re humane mononukle{\"a}re Zellen des peripheren Blutes. Die in den proliferativen Effekt der Stowellentherapie involvierte Signalkaskade konnte darauffolgend erstmals sowohl in murinen C3H10T1/2 Zellen als auch in humanen Fettstammzellen (adipose tissue-derived stem cells) und in humanen Jurkat T-Zellen detailliert beschrieben werden. Außerdem wurde ATP als entscheidendes Signalmolek{\"u}l identifiziert, welches nach Freisetzung durch Stoßwellenbehandlung mittels purinergem Signaling die Erk1/2 Signalkaskade aktiviert. F{\"u}r die dadurch gesteigerte Proliferation in stoßwellenbehandelten Zellen ist die Erk1/2 Aktivierung essentiell. In einem in vivo Model f{\"u}r gest{\"o}rte Wundheilung in der Ratte wurde die Hypothese zur entscheidenden Rolle des Erk1/2 Signalweges im wundheilungsf{\"o}rdernden Effekt der Stoßwelle best{\"a}tigt. In dieser Dissertation wurde gezeigt, dass die durch purinerges Signaling aktivierte Erk1/2 Signalkaskade eine entscheidende Rolle in der durch Stoßwellentherapie beschleunigten Zellproliferation in vitro und Wundheilung in vivo {\"u}bernimmt. Das damit erweiterte Verst{\"a}ndnis der Wirkungsmechanismen der Stoßwellentherapie kann zur Weiterentwicklung dieser Behandlungsform als zuk{\"u}nftige Standardtherapie bei Wundheilungsst{\"o}rungen, wie z.B. f{\"u}r diabetische oder chronische Wunden, beitragen.},
  subject      = {Healing},
  language  = {en}
}
@misc{Weihs,
  author    = {Weihs, Anna},
  title     = {Aufkl{\"a}rung des Wirkungsmechanismus der Stoßwellentherapie in der Wundheilung},
  subject      = {Shockwave},
  language  = {de}
}
@article{WeihsFuchsTeuschletal.,
  author    = {Weihs, Anna and Fuchs, Christiane and Teuschl, Andreas and Hartinger, Joachim and Slezak, Paul and Mittermayr, Rainer and Redl, Heinz and Junger, Wolfgang and Sitte, Harald and R{\"u}nzler, Dominik},
  title     = {Shock Wave Treatment Enhances Cell Proliferation and Improves Wound Healing by ATP Release-coupled Extracellular Signal-regulated Kinase (ERK) Activation},
  series = {The Journal of biological chemistry},
  journal   = {The Journal of biological chemistry},
  subject      = {Shockwave},
  language  = {de}
}
@article{SchuhHeherWeihsetal.,
  author    = {Schuh, Christina and Heher, Philipp and Weihs, Anna and Asmita, Banerjee and Wolbank, Susanne and Mittermayr, Rainer and Redl, Heinz and R{\"u}nzler, Dominik and Teuschl, Andreas},
  title     = {Adipose derived stem cells respond to in vitro extracorporeal shockwave treatment with increased stemness and multipotency},
  series = {New Biotechnology},
  journal   = {New Biotechnology},
  subject      = {Shockwave},
  language  = {en}
}
@misc{Weihs,
  author    = {Weihs, Anna},
  title     = {Revealing underlying mechanisms of shock wave treatment induced wound healing},
  subject      = {Shockwave},
  language  = {en}
}
@article{RiederWeihsTeuschletal.,
  author    = {Rieder, Bernhard and Weihs, Anna and Teuschl, Andreas and Knebl, Gerald and Kollmitzer, Josef and Redl, Heinz and R{\"u}nzler, Dominik},
  title     = {Evaluation of cell response on permanent and pulsed atmospheric pressure stressed cells},
  series = {Journal of Tissue Engineering and Regenerative Medicine},
  volume    = {1},
  journal   = {Journal of Tissue Engineering and Regenerative Medicine},
  number    = {6},
  pages     = {240 -- 240},
  subject      = {Cells},
  language  = {en}
}
@article{WeihsJungerSchadenetal.,
  author    = {Weihs, Anna and Junger, Wolfgang and Schaden, Wolfgang and Sitte, Harald and R{\"u}nzler, Dominik},
  title     = {Extracorporeal shockwave treatment induced extracellular ATP release - a potential mechanism to activate wound healing},
  series = {Journal of Tissue Engineering and Regenerative Medicine},
  volume    = {1},
  journal   = {Journal of Tissue Engineering and Regenerative Medicine},
  number    = {6},
  pages     = {381 -- 381},
  subject      = {Shockwave treatment},
  language  = {en}
}
@article{RuenzlerWeihsJungeretal.,
  author    = {R{\"u}nzler, Dominik and Weihs, Anna and Junger, Wolfgang and Sitte, Harald and Schaden, Wolfgang},
  title     = {Extracorporeal shock waves - a novel method of mechanostimulation in tissue regeneration},
  series = {Journal of Tissue Engineering and Regenerative Medicine},
  volume    = {1},
  journal   = {Journal of Tissue Engineering and Regenerative Medicine},
  number    = {6},
  pages     = {380 -- 380},
  subject      = {Shockwave treatment},
  language  = {en}
}
@misc{WeihsJungerSchadenetal.,
  author    = {Weihs, Anna and Junger, Wolgang G. and Schaden, Wolfgang and Sitte, Harald H. and R{\"u}nzler, Dominik},
  title     = {Extracellular ATP release - a potential benefit in shock wave treatment},
  subject      = {Shockwave},
  language  = {en}
}
@inproceedings{WeihsKneblRedletal.,
  author    = {Weihs, Anna and Knebl, Gerald and Redl, Heinz and R{\"u}nzler, Dominik},
  title     = {Evaluation of cell migration methods in 3D hydrogels for tissue engineering applications},
  series = {3. Forschungsforum der {\"o}sterreichischen Fachhochschulen / Fachhochschule K{\"a}rnten},
  booktitle = {3. Forschungsforum der {\"o}sterreichischen Fachhochschulen / Fachhochschule K{\"a}rnten},
  isbn      = {978-3-853912850},
  pages     = {490 -- 491},
  subject      = {Cells},
  language  = {en}
}
@misc{Weihs,
  author    = {Weihs, Anna},
  title     = {Establishment of an in vitro experimental set-up to evaluate the biological effects of extracorporeal shock wave treatment},
  subject      = {Shockwave},
  language  = {en}
}
@inproceedings{KneblWeihsWeingantetal.,
  author    = {Knebl, Gerald and Weihs, Anna and Weingant, Michaela and Steininger, Thomas and Redl, Heinz and R{\"u}nzler, Dominik},
  title     = {Automatisierte Datenauswertung eines Boyden-Mikro-Chemotaxis-Kammer Zell-Migrations Assays.},
  series = {2. Forschungsforum der {\"o}sterreichischen Fachhochschulen},
  booktitle = {2. Forschungsforum der {\"o}sterreichischen Fachhochschulen},
  isbn      = {978-3-8322-7023-0},
  pages     = {412 -- 417},
  subject      = {Cells},
  language  = {de}
}
@misc{HromadaTomaschWeihsetal.,
  author    = {Hromada, Carina and Tomasch, Janine and Weihs, Anna and R{\"u}nzler, Dominik and Teuschl, Andreas},
  title     = {Engineering of 3D Tissue Constructs Using our Novel MagneTissue Bioreactor as Alternatives to Animal Models},
  subject      = {Bioreactor},
  language  = {en}
}
@article{RiederWeihsWeidingeretal.,
  author    = {Rieder, Bernhard and Weihs, Anna and Weidinger, Adelheid and Sczwarc, Dorota and N{\"u}rnberger, Sylvia and Redl, Heinz and R{\"u}nzler, Dominik and Huber-Gries, Carina and Teuschl, Andreas},
  title     = {Hydrostatic pressure-generated reactive oxygen species induce osteoarthritic conditions in cartilage pellet cultures},
  series = {Scientific Reports},
  journal   = {Scientific Reports},
  subject      = {Bioreactor},
  language  = {en}
}
@article{JohannesWeihsKarneretal.,
  author    = {Johannes, Hackethal and Weihs, Anna and Karner, Lisa and Metzger, Magdalena and Dungel, Peter and Hennerbichler, Simone and Redl, Heinz and Teuschl-Woller, Andreas Herbert},
  title     = {Novel Human Placenta-Based Extract for Vascularization Strategies in Tissue Engineering},
  series = {Tissue Eng Part C Methods},
  volume    = {27},
  journal   = {Tissue Eng Part C Methods},
  number    = {11},
  pages     = {616 -- 632},
  abstract  = {There is critical unmet need for new vascularized tissues to support or replace injured tissues and organs. Various synthetic and natural materials were already established for use of two-dimensional (2D) and three-dimensional (3D) in vitro neovascularization assays, however, they still cannot mimic the complex functions of the sum of the extracellular matrix (ECM) in native intact tissue. Currently, this issue is only addressed by artificial products such as Matrigel™, which comprises a complex mixture of ECM proteins, extracted from animal tumor tissue. Despite its outstanding bioactivity, the isolation from tumor tissue hinders its translation into clinical applications. Since nonhuman ECM proteins may cause immune reactions, as are frequently observed in clinical trials, human ECM proteins represent the best option when aiming for clinical applications. Here, we describe an effective method of isolating a human placenta substrate (hpS) that induces the spontaneous formation of an interconnected network of green fluorescence-labeled human umbilical vein endothelial cells (gfpHUVECs) in vitro. The substrate was biochemically characterized by using a combination of bicinchoninic acid (BCA) assay, DNA, and glycosaminoglycan (GAG) content assays, sodium dodecyl sulfate/polyacrylamide gel electrophoresis (SDS-PAGE) analysis and Western blot, angiogenesis arrays, chromatographic thrombin detection, high performance liquid chromatography (HPLC)-based amino acid quantification analysis, and assessment of antimicrobial properties. 2D in vitro cell culture experiments have been performed to determine the vasculogenic potential of hpS, which demonstrated that cell networks developed on hpS show a significantly higher degree of complexity (number of tubules/junctions; total/mean tube length) when compared with Matrigel. As 3D cell culture techniques represent a more accurate representation of the in vivo condition, the substrate was 3D solidified using various natural polymers. 3D in vitro vasculogenesis assays have been performed by seeding gfpHUVECs in an hpS-fibrinogen clot. In conclusion, hpS provides a potent human/material-based alternative to xenogenic-material-based biomaterials for vascularization strategies in tissue engineering.},
  subject      = {Tissue Engineering},
  language  = {en}
}
@article{RohringerHolnthonerHackletal.,
  author    = {Rohringer, Sabrina and Holnthoner, Wolfgang and Hackl, Matthias and Weihs, Anna and R{\"u}nzler, Dominik and Skalicky, Susanna and Karbiener, Michael and Scheideler, Marcel and Pr{\"o}ll, Johannes and Gabriel, Christian and Schweighofer, Bernhard and Gr{\"o}ger, Marion and Spittler, Andreas and Grillari, Johannes and Redl, Heinz},
  title     = {Molecular and cellular effects of in vitro shockwave treatment on lymphatic endothelial cells.},
  series = {PLoS one},
  journal   = {PLoS one},
  subject      = {Shockwave},
  language  = {en}
}
@article{GollmannTepekoeylueGraberHirschetal.,
  author    = {Gollmann-Tepek{\"o}yl{\"u}, Can and Graber, Michael and Hirsch, Jakob and Mair, Sophia and Naschberger, Andreas and P{\"o}lzl, Leo and N{\"a}gele, Felix and Kirchmair, Elke and Degenhart, Gerald and Demetz, Egon and Hilbe, Richard and Chen, Hao-Yu and Engert, James C and B{\"o}hm, Anna and Franz, Nadja and Lobenwein, Daniela and Lener, Daniela and Fuchs, Christiane and Weihs, Anna and T{\"o}chterle, Sonja and Vogel, Georg F and Schweiger, Victor and Eder, Jonas and Pietschmann, Peter and Seifert, Markus and Kronenberg, Florian and Coassin, Stefan and Blumer, Michael and Hackl, Hubert and Meyer, Dirk and Feuchtner, Gudrun and Kirchmair, Rudolf and Troppmair, Jakob and Krane, Markus and Weiss, G{\"u}nther and Tsimikas, Sotirios and Thanassoulis, George and Grimm, Michael and Rupp, Bernhard and Huber, Lukas A and Zhang, Shen-Ying and Casanova, Jean-Laurent and Tancevski, Ivan and Holfeld, Johannes},
  title     = {Toll-Like Receptor 3 Mediates Aortic Stenosis Through a Conserved Mechanism of Calcification},
  series = {Circulation},
  volume    = {147},
  journal   = {Circulation},
  number    = {20},
  doi       = {10.1161/CIRCULATIONAHA.122.063481},
  pages     = {1518 -- 1533},
  subject      = {Toll-like receptor 3},
  language  = {en}
}