@article{SlezakSlezakHartingeretal.,
  author    = {Slezak, Paul and Slezak, Cyrill and Hartinger, Joachim and Teuschl, Andreas and N{\"u}rnberger, Sylvia and Redl, Heinz and Mittermayr, Rainer},
  title     = {A Low Cost Implantation Model in the Rat That Allows a Spatial Assessment of Angiogenesis.},
  series = {Frontiers in Bioengineering and Biotechnology},
  journal   = {Frontiers in Bioengineering and Biotechnology},
  abstract  = {There is continual demand for animal models that allow a quantitative assessment of angiogenic properties of biomaterials, therapies, and pharmaceuticals. In its simplest form, this is done by subcutaneous material implantation and subsequent vessel counting which usually omits spatial data. We have refined an implantation model and paired it with a computational analytic routine which outputs not only vessel count but also vessel density, distribution, and vessel penetration depth, that relies on a centric vessel as a reference point. We have successfully validated our model by characterizing the angiogenic potential of a fibrin matrix in conjunction with recombinant human vascular endothelial growth factor (rhVEGF165). The inferior epigastric vascular pedicles of rats were sheathed with silicone tubes, which were subsequently filled with 0.2 ml of fibrin and different doses of rhVEGF165, centrically embedding the vessels. Over 4 weeks, tissue samples were harvested and subsequently immunohistologically stained and computationally analyzed. The model was able to detect variations over the angiogenic potentials of growth factor spiked fibrin matrices. Adding 20 ng of rhVEGF165 resulted in a significant increase in vasculature while 200 ng of rhVEGF165 did not improve vascular growth. Vascularized tissue volume increased during the first week and vascular density increased during the second week. Total vessel count increased significantly and exhibited a peak after 2 weeks which was followed by a resorption of vasculature by week 4. In summary, a simple implantation model to study in vivo vascularization with only a minimal workload attached was enhanced to include morphologic data of the emerging vascular tree.},
  subject      = {Tissue Engineering},
  language  = {en}
}
@misc{SlezakRoseHercheretal.,
  author    = {Slezak, Paul and Rose, Roland and Hercher, David and Weihs, Anna and Fuchs, Christiane and Redl, Heinz and Mittermayr, Rainer and Slezak, Cyrill},
  title     = {Tracking therapeutic shockwaves and their impact on regeneration},
  subject      = {Shockwave Therapy},
  language  = {en}
}
@article{HeinzelOberhauserKeibletal.,
  author    = {Heinzel, Johannes Christoph and Oberhauser, Viola and Keibl, Claudia and Sch{\"a}dl, Barbara and Swiadek, Nicole V. and L{\"a}ngle, Gregor and Frick, Helen and Slezak, Cyrill and Prahm, Cosima and Grillari, Johannes and Kolbenschlag, Jonas and Hercher, David},
  title     = {ESWT Diminishes Axonal Regeneration following Repair of the Rat Median Nerve with Muscle-In-Vein Conduits but Not after Autologous Nerve Grafting},
  series = {Biomedicines},
  volume    = {2022},
  journal   = {Biomedicines},
  number    = {10(8)},
  pages     = {1777},
  abstract  = {Investigations reporting positive effects of extracorporeal shockwave therapy (ESWT) on nerve regeneration are limited to the rat sciatic nerve model. The effects of ESWT on muscle-in-vein conduits (MVCs) have also not been investigated yet. This study aimed to evaluate the effects of ESWT after repair of the rat median nerve with either autografts (ANGs) or MVCs. In male Lewis rats, a 7 mm segment of the right median nerve was reconstructed either with an ANG or an MVC. For each reconstructive technique, one group of animals received one application of ESWT while the other rats served as controls. The animals were observed for 12 weeks, and nerve regeneration was assessed using computerized gait analysis, the grasping test, electrophysiological evaluations and histological quantification of axons, blood vessels and lymphatic vasculature. Here, we provide for the first time a comprehensive analysis of ESWT effects on nerve regeneration in a rat model of median nerve injury. Furthermore, this study is among the first reporting the quantification of lymphatic vessels following peripheral nerve injury and reconstruction in vivo. While we found no significant direct positive effects of ESWT on peripheral nerve regeneration, results following nerve repair with MVCs were significantly inferior to those after ANG repair.},
  subject      = {Tissue Engineering},
  language  = {en}
}