@article{TomaschMaleinerHeheretal.,
  author    = {Tomasch, Janine and Maleiner, Babette and Heher, Philipp and Rufin, Manuel and Andriotis, Orestis G. and Thurner, Philipp J. and Redl, Heinz and Fuchs, Christiane and Teuschl-Woller, Andreas H.},
  title     = {Changes in Elastic Moduli of Fibrin Hydrogels Within the Myogenic Range Alter Behavior of Murine C2C12 and Human C25 Myoblasts Differently},
  series = {Froniers in Bioengineering and Biotechnology},
  volume    = {10},
  journal   = {Froniers in Bioengineering and Biotechnology},
  pages     = {836520},
  abstract  = {Fibrin hydrogels have proven highly suitable scaffold materials for skeletal muscle tissue engineering in the past. Certain parameters of those types of scaffolds, however, greatly affect cellular mechanobiology and therefore the myogenic outcome. The aim of this study was to identify the influence of apparent elastic properties of fibrin scaffolds in 2D and 3D on myoblasts and evaluate if those effects differ between murine and human cells. Therefore, myoblasts were cultured on fibrin-coated multiwell plates ("2D") or embedded in fibrin hydrogels ("3D") with different elastic moduli. Firstly, we established an almost linear correlation between hydrogels' fibrinogen concentrations and apparent elastic moduli in the range of 7.5 mg/ml to 30 mg/ml fibrinogen (corresponds to a range of 7.7-30.9 kPa). The effects of fibrin hydrogel elastic modulus on myoblast proliferation changed depending on culture type (2D vs 3D) with an inhibitory effect at higher fibrinogen concentrations in 3D gels and vice versa in 2D. The opposite effect was evident in differentiating myoblasts as shown by gene expression analysis of myogenesis marker genes and altered myotube morphology. Furthermore, culture in a 3D environment slowed down proliferation compared to 2D, with a significantly more pronounced effect on human myoblasts. Differentiation potential was also substantially impaired upon incorporation into 3D gels in human, but not in murine, myoblasts. With this study, we gained further insight in the influence of apparent elastic modulus and culture type on cellular behavior and myogenic outcome of skeletal muscle tissue engineering approaches. Furthermore, the results highlight the need to adapt parameters of 3D culture setups established for murine cells when applied to human cells.},
  subject      = {Tissue Engineering},
  language  = {en}
}
@article{MaleinerTomaschHeheretal.,
  author    = {Maleiner, Babette and Tomasch, Janine and Heher, Philipp and Spadiut, Oliver and R{\"u}nzler, Dominik and Fuchs, Christiane},
  title     = {The Importance of Biophysical and Biochemical Stimuli in Dynamic Skeletal Muscle Models.},
  series = {Frontiers in Physiology},
  journal   = {Frontiers in Physiology},
  abstract  = {Classical approaches to engineer skeletal muscle tissue based on current regenerative and surgical procedures still do not meet the desired outcome for patient applications. Besides the evident need to create functional skeletal muscle tissue for the repair of volumetric muscle defects, there is also growing demand for platforms to study muscle-related diseases, such as muscular dystrophies or sarcopenia. Currently, numerous studies exist that have employed a variety of biomaterials, cell types and strategies for maturation of skeletal muscle tissue in 2D and 3D environments. However, researchers are just at the beginning of understanding the impact of different culture settings and their biochemical (growth factors and chemical changes) and biophysical cues (mechanical properties) on myogenesis. With this review we intend to emphasize the need for new in vitro skeletal muscle (disease) models to better recapitulate important structural and functional aspects of muscle development. We highlight the importance of choosing appropriate system components, e.g., cell and biomaterial type, structural and mechanical matrix properties or culture format, and how understanding their interplay will enable researchers to create optimized platforms to investigate myogenesis in healthy and diseased tissue. Thus, we aim to deliver guidelines for experimental designs to allow estimation of the potential influence of the selected skeletal muscle tissue engineering setup on the myogenic outcome prior to their implementation. Moreover, we offer a workflow to facilitate identifying and selecting different analytical tools to demonstrate the successful creation of functional skeletal muscle tissue. Ultimately, a refinement of existing strategies will lead to further progression in understanding important aspects of muscle diseases, muscle aging and muscle regeneration to improve quality of life of patients and enable the establishment of new treatment options.},
  subject      = {Bioreactor},
  language  = {en}
}
@misc{HeherTomaschMaleineretal.,
  author    = {Heher, Philipp and Tomasch, Janine and Maleiner, Babette and Redl, Heinz and Fuchs, Christiane},
  title     = {The Importance of Biomechanical Cues for In Vitro Skeletal Myogenesis},
  subject      = {In Vitro},
  language  = {en}
}
@article{SchuhHeherWeihsetal.,
  author    = {Schuh, Christina and Heher, Philipp and Weihs, Anna and Fuchs, Christiane and Gabriel, Christian and Wolbank, Susanne and Mittermayr, Rainer and Redl, Heinz and R{\"u}nzler, Dominik and Teuschl, Andreas},
  title     = {In vitro extracorporeal shock wave treatment enhances stemness and preserves multipotency of rat and human adipose-derived stem cells},
  series = {Journal of Cytotherapy},
  journal   = {Journal of Cytotherapy},
  subject      = {Shockwave},
  language  = {en}
}
@inproceedings{MaleinerHeherTeuschletal.,
  author    = {Maleiner, Babette and Heher, Philipp and Teuschl, Andreas and Redl, Heinz and R{\"u}nzler, Dominik and Fuchs, Christiane},
  title     = {Generation of aligned skeletal muscle-like tissue based on the application of strain to a 3D fibrin scaffold},
  series = {Proceedings of PACT "Designer Cells go Clinical" Symposium},
  booktitle = {Proceedings of PACT "Designer Cells go Clinical" Symposium},
  subject      = {Tissue Generation},
  language  = {en}
}
@misc{MaleinerHeherTeuschletal.,
  author    = {Maleiner, Babette and Heher, Philipp and Teuschl, Andreas and Redl, Heinz and R{\"u}nzler, Dominik and Fuchs, Christiane},
  title     = {Generation of aligned skeletal muscle-like tissue based on the application of strain to a 3D fibrin scaffold},
  subject      = {Tissue Generation},
  language  = {en}
}
@article{HeherMaleinerPruelleretal.,
  author    = {Heher, Philipp and Maleiner, Babette and Pr{\"u}ller, Johanna and Teuschl, Andreas and Kollmitzer, Josef and Monforte Vila, Xavier and Wolbank, Susanne and Redl, Heinz and R{\"u}nzler, Dominik and Fuchs, Christiane},
  title     = {A novel bioreactor for the generation of highly aligned 3D skeletal muscle-like constructs through orientation of fibrin via application of static strain},
  series = {Acta Biomaterialia},
  journal   = {Acta Biomaterialia},
  subject      = {Bioreactor},
  language  = {en}
}
@misc{HeherFuchsPruelleretal.,
  author    = {Heher, Philipp and Fuchs, Christiane and Pr{\"u}ller, Johanna and Babette, Maleiner and R{\"u}nzler, Dominik and Redl, Heinz},
  title     = {A novel bioreactor for engineering skeletal muscle-like tissue in a strained fibrin scaffold},
  subject      = {Bioreactor},
  language  = {en}
}
@misc{TeuschlFuchsFeichtingeretal.,
  author    = {Teuschl, Andreas and Fuchs, Christiane and Feichtinger, Georg and Heher, Philipp and Heimel, Patrick and Schuh, Christina and N{\"u}rnberger, Sylvia and Nau, Thomas and R{\"u}nzler, Dominik and Redl, Heinz},
  title     = {Fibrin or Fibroin - Not only the "o" Makes the Difference},
  subject      = {Fibrin},
  language  = {en}
}
@article{SchuhHeherWeihsetal.,
  author    = {Schuh, Christina and Heher, Philipp and Weihs, Anna and Asmita, Banerjee and Wolbank, Susanne and Mittermayr, Rainer and Redl, Heinz and R{\"u}nzler, Dominik and Teuschl, Andreas},
  title     = {Adipose derived stem cells respond to in vitro extracorporeal shockwave treatment with increased stemness and multipotency},
  series = {New Biotechnology},
  journal   = {New Biotechnology},
  subject      = {Shockwave},
  language  = {en}
}
@misc{HeherFuchsPruelleretal.,
  author    = {Heher, Philipp and Fuchs, Christiane and Pr{\"u}ller, Johanna and Maleiner, Babette and Kollmitzer, Josef and R{\"u}nzler, Dominik and Teuschl, Andreas and Wolbank, Susanne and Redl, Heinz},
  title     = {A bioreactor-based 3D culture system for skeletal muscle engineering in fibrin scaffolds},
  subject      = {Bioreactors},
  language  = {en}
}
@article{SimsaPadmaHeheretal.,
  author    = {Simsa, Robin and Padma, Arvind and Heher, Philipp and Hellstr{\"o}m, Mats and Teuschl, Andreas and Jenndahl, Lachmi and Bergh, Niklas and Fogelstrand, Per},
  title     = {Systematic in vitro comparison of decellularization protocols for blood vessels.},
  series = {PLoS One},
  journal   = {PLoS One},
  subject      = {Tissue Engineering},
  language  = {en}
}