@article{PriglingerSchuhSteffenhagenetal., author = {Priglinger, Eleni and Schuh, Christina and Steffenhagen, Carolin and Wurzer, Christoph and Maier, Julia and N{\"u}rnberger, Sylvia and Holnthoner, Wolfgang and Fuchs, Christiane and Suessner, Susanne and R{\"u}nzler, Dominik and Redl, Heinz and Wolbank, Susanne}, title = {Improvement of adipose tissue-derived cells by low-energy extracorporeal shock wave therapy.}, series = {Cytotherapy}, journal = {Cytotherapy}, pages = {1079 -- 1095}, abstract = {BACKGROUND: Cell-based therapies with autologous adipose tissue-derived cells have shown great potential in several clinical studies in the last decades. The majority of these studies have been using the stromal vascular fraction (SVF), a heterogeneous mixture of fibroblasts, lymphocytes, monocytes/macrophages, endothelial cells, endothelial progenitor cells, pericytes and adipose-derived stromal/stem cells (ASC) among others. Although possible clinical applications of autologous adipose tissue-derived cells are manifold, they are limited by insufficient uniformity in cell identity and regenerative potency. METHODS: In our experimental set-up, low-energy extracorporeal shock wave therapy (ESWT) was performed on freshly obtained human adipose tissue and isolated adipose tissue SVF cells aiming to equalize and enhance stem cell properties and functionality. RESULTS: After ESWT on adipose tissue we could achieve higher cellular adenosine triphosphate (ATP) levels compared with ESWT on the isolated SVF as well as the control. ESWT on adipose tissue resulted in a significantly higher expression of single mesenchymal and vascular marker compared with untreated control. Analysis of SVF protein secretome revealed a significant enhancement in insulin-like growth factor (IGF)-1 and placental growth factor (PLGF) after ESWT on adipose tissue. DISCUSSION: Summarizing we could show that ESWT on adipose tissue enhanced the cellular ATP content and modified the expression of single mesenchymal and vascular marker, and thus potentially provides a more regenerative cell population. Because the effectiveness of autologous cell therapy is dependent on the therapeutic potency of the patient's cells, this technology might raise the number of patients eligible for autologous cell transplantation.}, subject = {Shockwave Therapy}, language = {en} } @article{SchuhHeherWeihsetal., author = {Schuh, Christina and Heher, Philipp and Weihs, Anna and Fuchs, Christiane and Gabriel, Christian and Wolbank, Susanne and Mittermayr, Rainer and Redl, Heinz and R{\"u}nzler, Dominik and Teuschl, Andreas}, title = {In vitro extracorporeal shock wave treatment enhances stemness and preserves multipotency of rat and human adipose-derived stem cells}, series = {Journal of Cytotherapy}, journal = {Journal of Cytotherapy}, subject = {Shockwave}, language = {en} } @article{BerkovitchCohenPeledetal., author = {Berkovitch, Yulia and Cohen, Talia and Peled, Eli and Schmidhammer, Robert and Hildner, Florian and Teuschl, Andreas and Wolbank, Susanne and Yelin, Dvir and Redl, Heinz and Seliktar, Dror}, title = {Hydrogel composition and laser micropatterning to regulate sciatic nerve regeneration.}, series = {Journal of Tissue Engineering and Regenerative Medicine}, journal = {Journal of Tissue Engineering and Regenerative Medicine}, pages = {1049 -- 1061}, abstract = {Treatment of peripheral nerve injuries has evolved over the past several decades to include the use of sophisticated new materials endowed with trophic and topographical cues that are essential for in vivo nerve fibre regeneration. In this research, we explored the use of an advanced design strategy for peripheral nerve repair, using biological and semi-synthetic hydrogels that enable controlled environmental stimuli to regenerate neurons and glial cells in a rat sciatic nerve resection model. The provisional nerve growth conduits were composed of either natural fibrin or adducts of synthetic polyethylene glycol and fibrinogen or gelatin. A photo-patterning technique was further applied to these 3D hydrogel biomaterials, in the form of laser-ablated microchannels, to provide contact guidance for unidirectional growth following sciatic nerve injury. We tested the regeneration capacity of subcritical nerve gap injuries in rats treated with photo-patterned materials and compared these with injuries treated with unpatterned hydrogels, either stiff or compliant. Among the factors tested were shear modulus, biological composition, and micropatterning of the materials. The microchannel guidance patterns, combined with appropriately matched degradation and stiffness properties of the material, proved most essential for the uniform tissue propagation during the nerve regeneration process.}, subject = {Tissue Engineering}, language = {en} } @article{BerkovitchCohenPeledetal., author = {Berkovitch, Yulia and Cohen, Talia and Peled, Eli and Schmidhammer, Robert and Hildner, Florian and Teuschl, Andreas and Wolbank, Susanne and Yelin, Dvir and Redl, Heinz and Seliktar, Dror}, title = {Hydrogel Composition and Laser Micro-Patterning to Regulate Sciatic Nerve Regeneration}, series = {Journal of Tissue Engineering and Regenerative Medicine}, volume = {12}, journal = {Journal of Tissue Engineering and Regenerative Medicine}, number = {4}, subject = {Micro-Patterning}, language = {en} } @article{SchneiderLehmannvanOschetal., author = {Schneider, Cornelia and Lehmann, Johannes and van Osch, Gerjo and Hildner, Florian and Teuschl, Andreas and Monforte Vila, Xavier and Miosga, David and Heimel, Patrick and Priglinger, Eleni and Redl, Heinz and Wolbank, Susanne and N{\"u}rnberger, Sylvia}, title = {Systematic Comparison of Protocols for the Preparation of Human Articular Cartilage for Use as Scaffold Material in Cartilage Tissue Engineering}, series = {Tissue Eng Part C Methods}, volume = {22}, journal = {Tissue Eng Part C Methods}, number = {12}, subject = {Cartilage}, language = {en} } @inproceedings{BasoliChaudryCrucianietal., author = {Basoli, Valentina and Chaudry, Sidrah and Cruciani, Sara and Fuchs, Christiane and Rieger, Sabine and Dungel, Peter and Wolbank, Susanne and Ventura, Carlo and Grillari-Voglauer, Regina and Redl, Heinz and Maioli, Margherita}, title = {Epigenetic and molecular behavious of stem cells exposed to biophysical stimuli: new insights in regenerative medicine}, series = {Proceedings des Seminars zum 40. Jahresjubil{\"a}um der {\"O}sterreichischen Gesellschaft f{\"u}r Chirurgische Forschung}, booktitle = {Proceedings des Seminars zum 40. Jahresjubil{\"a}um der {\"O}sterreichischen Gesellschaft f{\"u}r Chirurgische Forschung}, subject = {Stem Cells}, language = {en} } @misc{BasoliChaudryCrucianietal., author = {Basoli, Valentina and Chaudry, Sidrah and Cruciani, Gabriele and Fuchs, Christiane and Rieger, Sabine and Dungel, Peter and Wolbank, Susanne and Ventura, Carlo and Grillari-Voglauer, Regina and Redl, Heinz and Maioli, Margherita}, title = {Epigenetic and molecular behavious of stem cells exposed to biophysical stimuli: new insights in regenerative medicine}, subject = {Stem Cells}, language = {en} } @article{HeherMaleinerPruelleretal., author = {Heher, Philipp and Maleiner, Babette and Pr{\"u}ller, Johanna and Teuschl, Andreas and Kollmitzer, Josef and Monforte Vila, Xavier and Wolbank, Susanne and Redl, Heinz and R{\"u}nzler, Dominik and Fuchs, Christiane}, title = {A novel bioreactor for the generation of highly aligned 3D skeletal muscle-like constructs through orientation of fibrin via application of static strain}, series = {Acta Biomaterialia}, journal = {Acta Biomaterialia}, subject = {Bioreactor}, language = {en} } @article{SchuhHeherWeihsetal., author = {Schuh, Christina and Heher, Philipp and Weihs, Anna and Asmita, Banerjee and Wolbank, Susanne and Mittermayr, Rainer and Redl, Heinz and R{\"u}nzler, Dominik and Teuschl, Andreas}, title = {Adipose derived stem cells respond to in vitro extracorporeal shockwave treatment with increased stemness and multipotency}, series = {New Biotechnology}, journal = {New Biotechnology}, subject = {Shockwave}, language = {en} } @misc{HeherFuchsPruelleretal., author = {Heher, Philipp and Fuchs, Christiane and Pr{\"u}ller, Johanna and Maleiner, Babette and Kollmitzer, Josef and R{\"u}nzler, Dominik and Teuschl, Andreas and Wolbank, Susanne and Redl, Heinz}, title = {A bioreactor-based 3D culture system for skeletal muscle engineering in fibrin scaffolds}, subject = {Bioreactors}, language = {en} }