@misc{TeuschlFuchs,
  author    = {Teuschl, Andreas and Fuchs, Christiane},
  title     = {Bioreactors in Musculoskeletal Tissue Engineering},
  subject      = {Bioreactor},
  language  = {en}
}
@article{MaleinerTomaschHeheretal.,
  author    = {Maleiner, Babette and Tomasch, Janine and Heher, Philipp and Spadiut, Oliver and R{\"u}nzler, Dominik and Fuchs, Christiane},
  title     = {The Importance of Biophysical and Biochemical Stimuli in Dynamic Skeletal Muscle Models.},
  series = {Frontiers in Physiology},
  journal   = {Frontiers in Physiology},
  abstract  = {Classical approaches to engineer skeletal muscle tissue based on current regenerative and surgical procedures still do not meet the desired outcome for patient applications. Besides the evident need to create functional skeletal muscle tissue for the repair of volumetric muscle defects, there is also growing demand for platforms to study muscle-related diseases, such as muscular dystrophies or sarcopenia. Currently, numerous studies exist that have employed a variety of biomaterials, cell types and strategies for maturation of skeletal muscle tissue in 2D and 3D environments. However, researchers are just at the beginning of understanding the impact of different culture settings and their biochemical (growth factors and chemical changes) and biophysical cues (mechanical properties) on myogenesis. With this review we intend to emphasize the need for new in vitro skeletal muscle (disease) models to better recapitulate important structural and functional aspects of muscle development. We highlight the importance of choosing appropriate system components, e.g., cell and biomaterial type, structural and mechanical matrix properties or culture format, and how understanding their interplay will enable researchers to create optimized platforms to investigate myogenesis in healthy and diseased tissue. Thus, we aim to deliver guidelines for experimental designs to allow estimation of the potential influence of the selected skeletal muscle tissue engineering setup on the myogenic outcome prior to their implementation. Moreover, we offer a workflow to facilitate identifying and selecting different analytical tools to demonstrate the successful creation of functional skeletal muscle tissue. Ultimately, a refinement of existing strategies will lead to further progression in understanding important aspects of muscle diseases, muscle aging and muscle regeneration to improve quality of life of patients and enable the establishment of new treatment options.},
  subject      = {Bioreactor},
  language  = {en}
}
@article{TomaschMaleinerHeheretal.,
  author    = {Tomasch, Janine and Maleiner, Babette and Heher, Philipp and Rufin, Manuel and Andriotis, Orestis G. and Thurner, Philipp J. and Redl, Heinz and Fuchs, Christiane and Teuschl-Woller, Andreas H.},
  title     = {Changes in Elastic Moduli of Fibrin Hydrogels Within the Myogenic Range Alter Behavior of Murine C2C12 and Human C25 Myoblasts Differently},
  series = {Froniers in Bioengineering and Biotechnology},
  volume    = {10},
  journal   = {Froniers in Bioengineering and Biotechnology},
  pages     = {836520},
  abstract  = {Fibrin hydrogels have proven highly suitable scaffold materials for skeletal muscle tissue engineering in the past. Certain parameters of those types of scaffolds, however, greatly affect cellular mechanobiology and therefore the myogenic outcome. The aim of this study was to identify the influence of apparent elastic properties of fibrin scaffolds in 2D and 3D on myoblasts and evaluate if those effects differ between murine and human cells. Therefore, myoblasts were cultured on fibrin-coated multiwell plates ("2D") or embedded in fibrin hydrogels ("3D") with different elastic moduli. Firstly, we established an almost linear correlation between hydrogels' fibrinogen concentrations and apparent elastic moduli in the range of 7.5 mg/ml to 30 mg/ml fibrinogen (corresponds to a range of 7.7-30.9 kPa). The effects of fibrin hydrogel elastic modulus on myoblast proliferation changed depending on culture type (2D vs 3D) with an inhibitory effect at higher fibrinogen concentrations in 3D gels and vice versa in 2D. The opposite effect was evident in differentiating myoblasts as shown by gene expression analysis of myogenesis marker genes and altered myotube morphology. Furthermore, culture in a 3D environment slowed down proliferation compared to 2D, with a significantly more pronounced effect on human myoblasts. Differentiation potential was also substantially impaired upon incorporation into 3D gels in human, but not in murine, myoblasts. With this study, we gained further insight in the influence of apparent elastic modulus and culture type on cellular behavior and myogenic outcome of skeletal muscle tissue engineering approaches. Furthermore, the results highlight the need to adapt parameters of 3D culture setups established for murine cells when applied to human cells.},
  subject      = {Tissue Engineering},
  language  = {en}
}
@article{AngelovaDaskalovaFilipovetal.,
  author    = {Angelova, Liliya and Daskalova, Albena and Filipov, Emil and Monforte Vila, Xavier and Tomasch, Janine and Avdeev, Georgi and Teuschl-Woller, Andreas Herbert and Buchvarov, Ivan},
  title     = {Optimizing the Surface Structural and Morphological Properties of Silk Thin Films via Ultra-Short Laser Texturing for Creation of Muscle Cell Matrix Model},
  series = {Polymers},
  volume    = {2022},
  journal   = {Polymers},
  number    = {14(13), 2584},
  abstract  = {Temporary scaffolds that mimic the extracellular matrix's structure and provide a stable substratum for the natural growth of cells are an innovative trend in the field of tissue engineering. The aim of this study is to obtain and design porous 2D fibroin-based cell matrices by femtosecond laser-induced microstructuring for future applications in muscle tissue engineering. Ultra-fast laser treatment is a non-contact method, which generates controlled porosity-the creation of micro/nanostructures on the surface of the biopolymer that can strongly affect cell behavior, while the control over its surface characteristics has the potential of directing the growth of future muscle tissue in the desired direction. The laser structured 2D thin film matrices from silk were characterized by means of SEM, EDX, AFM, FTIR, Micro-Raman, XRD, and 3D-roughness analyses. A WCA evaluation and initial experiments with murine C2C12 myoblasts cells were also performed. The results show that by varying the laser parameters, a different structuring degree can be achieved through the initial lifting and ejection of the material around the area of laser interaction to generate porous channels with varying widths and depths. The proper optimization of the applied laser parameters can significantly improve the bioactive properties of the investigated 2D model of a muscle cell matrix. Keywords: biopolymers; femtosecond laser processing; muscle cell matrix 2D model; muscle tissue engineering; silk fibroin.},
  subject      = {Tissue Engineering},
  language  = {en}
}
@misc{HeherTomaschMaleineretal.,
  author    = {Heher, Philipp and Tomasch, Janine and Maleiner, Babette and Redl, Heinz and Fuchs, Christiane},
  title     = {The Importance of Biomechanical Cues for In Vitro Skeletal Myogenesis},
  subject      = {In Vitro},
  language  = {en}
}
@misc{Tomasch,
  author    = {Tomasch, Janine},
  title     = {Generation of 3D skeletal muscle-like scaffolds via the application of mechanical stimuli},
  subject      = {Scaffold},
  language  = {en}
}
@article{SchuhHeherWeihsetal.,
  author    = {Schuh, Christina and Heher, Philipp and Weihs, Anna and Fuchs, Christiane and Gabriel, Christian and Wolbank, Susanne and Mittermayr, Rainer and Redl, Heinz and R{\"u}nzler, Dominik and Teuschl, Andreas},
  title     = {In vitro extracorporeal shock wave treatment enhances stemness and preserves multipotency of rat and human adipose-derived stem cells},
  series = {Journal of Cytotherapy},
  journal   = {Journal of Cytotherapy},
  subject      = {Shockwave},
  language  = {en}
}
@incollection{KoehlerRuenzlerMayeretal.,
  author    = {K{\"o}hler, Gottfried and R{\"u}nzler, Dominik and Mayer, B. and S{\´a}ra, Margit and Rasmussen, S. and Buravkova, L.},
  title     = {Microgravity. Applications in Biotechnology and Bioorganic Materials Sciences},
  series = {10 Years Space biomedical Research and Development in Austria},
  booktitle = {10 Years Space biomedical Research and Development in Austria},
  publisher = {Facultas},
  address   = {Wien},
  publisher      = {Fachhochschule Technikum Wien},
  pages     = {65 -- 85},
  subject      = {Microgravity},
  language  = {en}
}
@article{PurtscherRothbauerKratzetal.,
  author    = {Purtscher, Michaela and Rothbauer, Mario and Kratz, Sebastian Rudi Adam and Bailey, Andrew and Lieberzeit, Peter and Ertl, Peter},
  title     = {A microfluidic impedance-based extended infectivity assay: combining retroviral amplification and cytopathic effect monitoring on a single lab-on-a-chip platform},
  series = {Lab on a Chip},
  volume    = {2021},
  journal   = {Lab on a Chip},
  number    = {Issue 7},
  pages     = {1364 -- 1372},
  abstract  = {Detection, quantification and monitoring of virus - host cell interactions are of great importance when evaluating the safety of pharmaceutical products. With the wide usage of viral based vector systems in combination with mammalian cell lines for the production of biopharmaceuticals, the presence of replication competent viral particles needs to be avoided and potential hazards carefully assessed. Consequently, regulatory agencies recommend viral clearance studies using plaque assays or TCID50 assays to evaluate the efficiency of the production process in removing viruses. While plaque assays provide reliable information on the presence of viral contaminations, they are still tedious to perform and can take up to two weeks to finish. To overcome some of these limitations, we have automated, miniaturized and integrated the dual cell culture bioassay into a common lab-on-a-chip platform containing embedded electrical sensor arrays to enrich and detect infectious viruses. Results of our microfluidic single step assay show that a significant reduction in assay time down to 3 to 4 days can be achieved using simultaneous cell-based viral amplification, release and detection of cytopathic effects in a target cell line. We further demonstrate the enhancing effect of continuous fluid flow on infection of PG-4 reporter cells by newly formed and highly active virions by M. dunni cells, thus pointing to the importance of physical relevant viral-cell interactions.},
  subject      = {Tissue Engineering},
  language  = {en}
}
@article{ZupkovitzKabiljoKothmayeretal.,
  author    = {Zupkovitz, Gordin and Kabiljo, Julijan and Kothmayer, Michael and Schlick, Katharina and Sch{\"o}fer, Christian and Lagger, Sabine and Pusch, Oliver},
  title     = {Analysis of Methylation Dynamics Reveals a Tissue-Specific, Age-Dependent Decline in 5-Methylcytosine Within the Genome of the Vertebrate Aging Model Nothobranchius furzeri.},
  series = {Frontiers in Molecular Biosciences},
  volume    = {8},
  journal   = {Frontiers in Molecular Biosciences},
  number    = {627143},
  abstract  = {Erosion of the epigenetic DNA methylation landscape is a widely recognized hallmark of aging. Emerging advances in high throughput sequencing techniques, in particular DNA methylation data analysis, have resulted in the establishment of precise human and murine age prediction tools. In vertebrates, methylation of cytosine at the C5 position of CpG dinucleotides is executed by DNA methyltransferases (DNMTs) whereas the process of enzymatic demethylation is highly dependent on the activity of the ten-eleven translocation methylcytosine dioxygenase (TET) family of enzymes. Here, we report the identification of the key players constituting the DNA methylation machinery in the short-lived teleost aging model Nothobranchius furzeri. We present a comprehensive spatio-temporal expression profile of the methylation-associated enzymes from embryogenesis into late adulthood, thereby covering the complete killifish life cycle. Data mining of the N. furzeri genome produced five dnmt gene family orthologues corresponding to the mammalian DNMTs (DNMT1, 2, 3A, and 3B). Comparable to other teleost species, N. furzeri harbors multiple genomic copies of the de novo DNA methylation subfamily. A related search for the DNMT1 recruitment factor UHRF1 and TET family members resulted in the identification of N. furzeri uhrf1, tet1, tet2, and tet3. Phylogenetic analysis revealed high cross-species similarity on the amino acid level of all individual dnmts, tets, and uhrf1, emphasizing a high degree of functional conservation. During early killifish development all analyzed dnmts and tets showed a similar expression profile characterized by a strong increase in transcript levels after fertilization, peaking either at embryonic day 6 or at the black eye stage of embryonic development. In adult N. furzeri, DNA methylation regulating enzymes showed a ubiquitous tissue distribution. Specifically, we observed an age-dependent downregulation of dnmts, and to some extent uhrf1, which correlated with a significant decrease in global DNA methylation levels in the aging killifish liver and muscle. The age-dependent DNA methylation profile and spatio-temporal expression characteristics of its enzymatic machinery reported here may serve as an essential platform for the identification of an epigenetic aging clock in the new vertebrate model system N. furzeri.},
  subject      = {Tissue Engineering},
  language  = {en}
}
@inproceedings{WiessnerMachacekLinkLeitner,
  author    = {Wießner, Katharina and Machacek-Link, Thomas and Leitner, Rita},
  title     = {Encouraging the development of higher-order cognitive skills via applied exercises and web-based self-assessment to teach the basic principles in molecular biology.},
  series = {PIXEL NPSE2021, M{\"a}rz 2021},
  booktitle = {PIXEL NPSE2021, M{\"a}rz 2021},
  abstract  = {The responsibility of a lecturer is not only to share his or her knowledge with the students in an easy to understand manner, but also to help the students to embed new knowledge and to encourage the development of higher-order cognitive skills via applied exercises. In order to meet the growing demand for blended learning approaches a new course concept was established in autumn 2018. To enhance comprehension and to provide opportunities for self-assessment, web-based training units were implemented by using the interactive learning software "Articulate Storyline". Students had to prepare at home for the course units by completing interactive chapters. Their learning outcome was assessed by online quizzes at the end of each chapter. Online Training chapters allowed time to focus on selected topics and to repeat key messages in following presence units. Additionally, guided group exercises were performed to promote analytic skills and abstract thinking. The students had to apply and combine their knowledge to solve problem-based challenges. An optional revision course was offered to the students, which allowed for interactive repetition of the acquired knowledge with the focus on student-to-lecturer dialog. An analysis based on a written evaluation of this course resulted in a positive feedback from the students, in particular regarding the guided exercises and the offered revision course. According to the students the group exercises allowed to process the learned subjects, promoted the group climate and were a convenient diversion from the frontal lecture format. Students who attended the revision course on a regular basis showed a better performance at the final exam and exceeded especially at interdisciplinary questions. The first implementation of this master´s degree course indicated that the combination of web-based training elements with frontal lecture elements, guided exercises stimulating cognitive skills and an optional revision course can teach students the basics of biology in an understandable way. This course structure is especially applicable to teach basic subjects for groups of students with varying initial knowledge. Financial support from the City of Vienna project PBL in Molecular Life Science (21-06) is gratefully acknowledged.},
  subject      = {PBL},
  language  = {en}
}
@article{DeiningerWagnerHeimeletal.,
  author    = {Deininger, Christian and Wagner, Andrea and Heimel, Patrick and Salzer, Elias and Monforte Vila, Xavier and Weißenbacher, Nadja and Grillari, Johannes and Redl, Heinz and Wichlas, Florian and Freude, Thomas and Tempfer, Herbert and Teuschl-Woller, Andreas and Traweger, Andreas},
  title     = {Enhanced BMP-2-Mediated Bone Repair Using an Anisotropic Silk Fibroin Scaffold Coated with Bone-like Apatite},
  series = {Int. J. Mol. Sci.},
  volume    = {23},
  journal   = {Int. J. Mol. Sci.},
  number    = {1 / 283},
  abstract  = {The repair of large bone defects remains challenging and often requires graft material due to limited availability of autologous bone. In clinical settings, collagen sponges loaded with excessive amounts of bone morphogenetic protein 2 (rhBMP-2) are occasionally used for the treatment of bone non-unions, increasing the risk of adverse events. Therefore, strategies to reduce rhBMP-2 dosage are desirable. Silk scaffolds show great promise due to their favorable biocompatibility and their utility for various biofabrication methods. For this study, we generated silk scaffolds with axially aligned pores, which were subsequently treated with 10× simulated body fluid (SBF) to generate an apatitic calcium phosphate coating. Using a rat femoral critical sized defect model (CSD) we evaluated if the resulting scaffold allows the reduction of BMP-2 dosage to promote efficient bone repair by providing appropriate guidance cues. Highly porous, anisotropic silk scaffolds were produced, demonstrating good cytocompatibility in vitro and treatment with 10× SBF resulted in efficient surface coating. In vivo, the coated silk scaffolds loaded with a low dose of rhBMP-2 demonstrated significantly improved bone regeneration when compared to the unmineralized scaffold. Overall, our findings show that this simple and cost-efficient technique yields scaffolds that enhance rhBMP-2 mediated bone healing.},
  subject      = {Tissue Engineering},
  language  = {en}
}
@article{HackethalDungelTeuschl,
  author    = {Hackethal, Johannes and Dungel, Peter and Teuschl, Andreas Herbert},
  title     = {Frequently Used Strategies to Isolate Extracellular Matrix Proteins from Human Placenta and Adipose Tissue},
  series = {Tissue Engineering Part C: Methods},
  volume    = {27},
  journal   = {Tissue Engineering Part C: Methods},
  number    = {12},
  pages     = {649 -- 660},
  abstract  = {The natural extracellular matrix (ECM) provides the optimal environment for cells. Many enzymatic or non-enzymatic based strategies to extract ECM proteins from tissues were published over the past years. However, every single isolation strategy reported so far is associated with specific bottlenecks. In this study, frequently used strategies to isolate ECM from human placenta or adipose tissue using Tris-, serum-, or pepsin-based buffers were compared. The resulting ECM proteins were biochemically characterized by analysis of cellular remnants using Hoechst DNA staining, glycosaminoglycan (GAG) content by dimethylmethylene blue, visualization of protein bands using sodium dodecyl sulfate-polyacrylamide gel electrophoresis analysis combined with amino acid quantification, and assessment of the proangiogenic profile using an angiogenesis array. Tris-NaCl-extracted ECM proteins showed a high heterogenic degree of extracted proteins, bioactive growth factors, and GAGs, but no collagen-I. Active serum-extracted ECM showed significant lower DNA remnants when compared with the Tris-NaCl isolation strategy. Pepsin-extracted ECM was rich in collagen-I and low amounts of remaining bioactive growth factors. This strategy was most effective to reduce DNA amounts when compared with the other isolation strategies. Pepsin-extracted ECM from both tissues easily gelled at 37°C, whereas the other extracted ECM strategies did not gel at 37°C (Tris-NaCl: liquid; serum: sponge). All relevant characteristics (DNA residues, ECM diversity and bioactivity, shape) of the extracted ECM proteins highly depend on its isolation strategy and could still be optimized. Impact statement The natural human extracellular matrix (ECM) is the ideal cell niche. Various strategies were reported to isolate human ECM components from various sources. In this article, we compared frequently used methods and compared their characteristics (DNA remnants, glycosaminoglycan content, sodium dodecyl sulfate-polyacrylamide gel electrophoresis analysis, amino acid quantification, angiogenesis array, and gel formation). We conclude that more research is still necessary to optimize current isolation approaches for in vitro or in vivo applications of human ECM.},
  subject      = {Tissue Engineering},
  language  = {en}
}
@article{KhimichProsolovMishurovaetal.,
  author    = {Khimich, Margarita A. and Prosolov, Konstantin A. and Mishurova, Tatiana and Evsevleev, Sergej and Monforte, Xavier and Teuschl, Andreas H. and Slezak, Paul and Ibragimov, Egor A. and Saprykin, Alexander A. and Kovalevskaya, Zhanna G. and Dmitriev, Andrey I. and Bruno, Giovanni and Sharkeev, Yurii P.},
  title     = {Advances in Laser Additive Manufacturing of Ti-Nb Alloys: From Nanostructured Powders to Bulk Objects},
  series = {Nanomaterials (Basel)},
  volume    = {11},
  journal   = {Nanomaterials (Basel)},
  number    = {5 / 1159},
  abstract  = {The additive manufacturing of low elastic modulus alloys that have a certain level of porosity for biomedical needs is a growing area of research. Here, we show the results of manufacturing of porous and dense samples by a laser powder bed fusion (LPBF) of Ti-Nb alloy, using two distinctive fusion strategies. The nanostructured Ti-Nb alloy powders were produced by mechanical alloying and have a nanostructured state with nanosized grains up to 90 nm. The manufactured porous samples have pronounced open porosity and advanced roughness, contrary to dense samples with a relatively smooth surface profile. The structure of both types of samples after LPBF is formed by uniaxial grains having micro- and nanosized features. The inner structure of the porous samples is comprised of an open interconnected system of pores. The volume fraction of isolated porosity is 2 vol. \% and the total porosity is 20 vol. \%. Cell viability was assessed in vitro for 3 and 7 days using the MG63 cell line. With longer culture periods, cells showed an increased cell density over the entire surface of a porous Ti-Nb sample. Both types of samples are not cytotoxic and could be used for further in vivo studies.},
  subject      = {Tissue Engineering},
  language  = {en}
}
@article{FarokhiAleemardaniSolouketal.,
  author    = {Farokhi, Maryam and Aleemardani, Mina and Solouk, Atefeh and Mirzadeh, Hamid and Teuschl, Andreas Herbert and Redl, Heinz},
  title     = {Crosslinking strategies for silk fibroin hydrogels: promising biomedical materials},
  series = {Biomedical Materials},
  volume    = {16},
  journal   = {Biomedical Materials},
  number    = {2},
  pages     = {022004},
  abstract  = {Due to their strong biomimetic potential, silk fibroin (SF) hydrogels are impressive candidates for tissue engineering, due to their tunable mechanical properties, biocompatibility, low immunotoxicity, controllable biodegradability, and a remarkable capacity for biomaterial modification and the realization of a specific molecular structure. The fundamental chemical and physical structure of SF allows its structure to be altered using various crosslinking strategies. The established crosslinking methods enable the formation of three-dimensional (3D) networks under physiological conditions. There are different chemical and physical crosslinking mechanisms available for the generation of SF hydrogels (SFHs). These methods, either chemical or physical, change the structure of SF and improve its mechanical stability, although each method has its advantages and disadvantages. While chemical crosslinking agents guarantee the mechanical strength of SFH through the generation of covalent bonds, they could cause some toxicity, and their usage is not compatible with a cell-friendly technology. On the other hand, physical crosslinking approaches have been implemented in the absence of chemical solvents by the induction of β-sheet conformation in the SF structure. Unfortunately, it is not easy to control the shape and properties of SFHs when using this method. The current review discusses the different crosslinking mechanisms of SFH in detail, in order to support the development of engineered SFHs for biomedical applications.},
  subject      = {Tissue Engineering},
  language  = {en}
}
@article{SchandaKeiblHeimeletal.,
  author    = {Schanda, Jakob and Keibl, Claudia and Heimel, Patrick and Monforte, Xavier and Feichtinger, Xaver and Teuschl, Andreas and Baierl, Andreas and Muschitz, Christian and Redl, Heinz and Fialka, Christian and Mittermayr, Rainer},
  title     = {Zoledronic Acid Substantially Improves Bone Microarchitecture and Biomechanical Properties After Rotator Cuff Repair in a Rodent Chronic Defect Model},
  series = {Am J Sports Med},
  volume    = {2020 Jul},
  journal   = {Am J Sports Med},
  number    = {48 (9)},
  pages     = {2151 -- 2160},
  abstract  = {Background: Bone mineral density at the humeral head is reduced in patients with chronic rotator cuff tears. Bone loss in the humeral head is associated with repair failure after rotator cuff reconstruction. Bisphosphonates (eg, zoledronic acid) increase bone mineral density. Hypothesis: Zoledronic acid improves bone mineral density of the humeral head and biomechanical properties of the enthesis after reconstruction of chronic rotator cuff tears in rats. Study design: Controlled laboratory study. Methods: A total of 32 male Sprague-Dawley rats underwent unilateral (left) supraspinatus tenotomy with delayed transosseous rotator cuff reconstruction after 3 weeks. All rats were sacrificed 8 weeks after rotator cuff repair. Animals were randomly assigned to 1 of 2 groups. At 1 day after rotator cuff reconstruction, the intervention group was treated with a single subcutaneous dose of zoledronic acid at 100 µg/kg bodyweight, and the control group received 1 mL of subcutaneous saline solution. In 12 animals of each group, micro-computed tomography scans of both shoulders were performed as well as biomechanical testing of the supraspinatus enthesis of both sides. In 4 animals of each group, histological analyses were conducted. Results: In the intervention group, bone volume fraction (bone volume/total volume [BV/TV]) of the operated side was higher at the lateral humeral head (P = .005) and the medial humeral head (P = .010) compared with the control group. Trabecular number on the operated side was higher at the lateral humeral head (P = .004) and the medial humeral head (P = .001) in the intervention group. Maximum load to failure rates on the operated side were higher in the intervention group (P < .001). Cortical thickness positively correlated with higher maximum load to failure rates in the intervention group (r = 0.69; P = .026). Histological assessment revealed increased bone formation in the intervention group. Conclusion: Single-dose therapy of zoledronic acid provided an improvement of bone microarchitecture at the humeral head as well as an increase of maximum load to failure rates after transosseous reconstruction of chronic rotator cuff lesions in rats. Clinical relevance: Zoledronic acid improves bone microarchitecture as well as biomechanical properties after reconstruction of chronic rotator cuff tears in rodents. These results need to be verified in clinical investigations.},
  subject      = {Tissue Engineering},
  language  = {en}
}
@article{BachmannSpitzSchaedletal.,
  author    = {Bachmann, Barbara and Spitz, Sarah and Sch{\"a}dl, Barbara and Teuschl, Andreas and Redl, Heinz and N{\"u}rnberger, Sylvia and Ertl, Peter},
  title     = {Stiffness Matters: Fine-Tuned Hydrogel Elasticity Alters Chondrogenic Redifferentiation},
  series = {Froniers in Bioengineering and Biotechnology},
  volume    = {2020},
  journal   = {Froniers in Bioengineering and Biotechnology},
  number    = {8},
  pages     = {373},
  abstract  = {Biomechanical cues such as shear stress, stretching, compression, and matrix elasticity are vital in the establishment of next generation physiological in vitro tissue models. Matrix elasticity, for instance, is known to guide stem cell differentiation, influence healing processes and modulate extracellular matrix (ECM) deposition needed for tissue development and maintenance. To better understand the biomechanical effect of matrix elasticity on the formation of articular cartilage analogs in vitro, this study aims at assessing the redifferentiation capacity of primary human chondrocytes in three different hydrogel matrices of predefined matrix elasticities. The hydrogel elasticities were chosen to represent a broad spectrum of tissue stiffness ranging from very soft tissues with a Young's modulus of 1 kPa up to elasticities of 30 kPa, representative of the perichondral-space. In addition, the interplay of matrix elasticity and transforming growth factor beta-3 (TGF-β3) on the redifferentiation of primary human articular chondrocytes was studied by analyzing both qualitative (viability, morphology, histology) and quantitative (RT-qPCR, sGAG, DNA) parameters, crucial to the chondrotypic phenotype. Results show that fibrin hydrogels of 30 kPa Young's modulus best guide chondrocyte redifferentiation resulting in a native-like morphology as well as induces the synthesis of physiologic ECM constituents such as glycosaminoglycans (sGAG) and collagen type II. This comprehensive study sheds light onto the mechanobiological impact of matrix elasticity on formation and maintenance of articular cartilage and thus represents a major step toward meeting the need for advanced in vitro tissue models to study both re- and degeneration of articular cartilage.},
  subject      = {Tissue Engineering},
  language  = {en}
}
@article{SimboeckMarksteinerMachaceketal.,
  author    = {Simb{\"o}ck, Elisabeth and Marksteiner, Jessica and Machacek, Thomas and Wiessner, Katharina and Gepp, Barbara and Jesenberger, Veronika and Weihs, Anna and Leitner, Rita},
  title     = {The Power of Problem Based Learning beyond its Didactic Attributes},
  series = {Journal of Problem Based Learning in Higher Education (JPBLHE)},
  volume    = {9},
  journal   = {Journal of Problem Based Learning in Higher Education (JPBLHE)},
  number    = {1},
  pages     = {109 -- 130},
  abstract  = {Hybrid courses with a focus on practice-orientated education and self-guided learning phases are on the rise on the higher education sector. Disciplines in Life Sciences implicate a high degree of practical laboratory expertise. The University of Applied Sciences (UAS) in Vienna, Austria, has thus been endeavoured offering students a high qualitative education integrating hybrid courses based on PBL principles, which consist of on-site (including the transmission of necessary background and practical laboratory training) and off-site (including self-study phases) sessions. As practical laboratory units are central in those courses, the restrictive measures, including the transition to a complete online teaching format due to the first Covid-19-pandemic lock-down, had severe effects on the implementation and the quality of the curriculum. According to surveys made specifically to address this problematic situation, it can be concluded that on-site practical units are fundamental for certain disciplines such as Life Sciences.},
  subject      = {Problem-based Learning},
  language  = {en}
}
@inproceedings{WiessnerPraherLiousiaetal.,
  author    = {Wießner, Katharina and Praher, Daniela and Liousia, Varvara and Gepp, Barbara and Leitner, Rita and R{\"u}nzler, Dominik},
  title     = {Testing the acute toxicity of an alternative to Glyphosate - Pelargonic acid},
  series = {SETAC Europe 30th Annual Meeting - Online},
  booktitle = {SETAC Europe 30th Annual Meeting - Online},
  organization    = {Society of Environmental Toxicology and Chemistry (SETAC)},
  abstract  = {Glyphosate-based herbicides were used as plant protection product globally for several decades. However, glyphosate is discussed as showing genotoxicity and many other side-effects such as inhibiting the mitochondrial succinate dehydrogenase, leading to a decreased ATP production. Therefore, finding alternative active substances is necessary. Pelargonic acid (nonanoic acid) and its ammonium salt (saponified form), which are used as alternatives to glyphosate-based herbicides, are biological derived substances considered as environmentally friendly herbicides. To test the effects of pelargonic acid in its acidic form (active substance in TopGun) and its saponified form (in Finalsan Plus) on the aquatic ecosystem, students of the master study program "environmental management and ecotoxicology" compared the toxicity levels of these substances using zebrafish embryos within the scope of a student research course. The project was developed by applying the 7-step problem-based learning method which allowed the students to design their experiments independently with the guidance and feedback of the lecturers. Acute toxicity was determined according to OECD test guideline 236 in D. rerio which revealed a LC50 of 1.55 mg/L of pelargonic acid, a LC50 value of 0.93 mg/L pelargonic acid in TopGun and a LC50 of 36.37 mg/L of Finalsan Plus. Neutral Red Uptake assays were performed on the rainbow trout-derived gill cell-line RTgill-W1 to determine the acute toxicity according to the OECD test guideline 129 which revealed the IC50 value of 12.4 mg/L pelargonic acid in TG. Due to solubility limits, no reliable IC50 could be obtained. The results of the tests indicate differences in the acute toxicity of the pelargonic acid-based formulations TopGun and Finalsan Plus. However, more investigations have to be done in order to analyse if the acidic form is more toxic than the saponified form. Furthermore, detailed ecotoxicological risk assessments and an evaluation of the genotoxicity of both herbicides has to be performed before any conclusion can be drawn. Financial support from the City of Vienna project PBL in Molecular Life Science (21-06) is gratefully acknowledged.},
  subject      = {Environmental Chemistry},
  language  = {en}
}
@article{ZiadlouRotmanTeuschletal.,
  author    = {Ziadlou, Reihane and Rotman, Stijn and Teuschl, Andreas and Salzer, Elias and Barbero, Andrea and Martin, Ivan and Alini, Mauro and Eglin, David and Grad, Sibylle},
  title     = {Optimization of hyaluronic acid-tyramine/silk-fibroin composite hydrogels for cartilage tissue engineering and delivery of anti-inflammatory and anabolic drugs},
  series = {Materials Science and Engineering: C},
  volume    = {120},
  journal   = {Materials Science and Engineering: C},
  number    = {111701},
  abstract  = {Injury of articular cartilage leads to an imbalance in tissue homeostasis, and due to the poor self-healing capacity of cartilage the affected tissue often exhibits osteoarthritic changes. In recent years, injectable and highly tunable composite hydrogels for cartilage tissue engineering and drug delivery have been introduced as a desirable alternative to invasive treatments. In this study, we aimed to formulate injectable hydrogels for drug delivery and cartilage tissue engineering by combining different concentrations of hyaluronic acid-tyramine (HA-Tyr) with regenerated silk-fibroin (SF) solutions. Upon enzymatic crosslinking, the gelation and mechanical properties were characterized over time. To evaluate the effect of the hydrogel compositions and properties on extracellular matrix (ECM) deposition, bovine chondrocytes were embedded in enzymatically crosslinked HA-Tyr/SF composites (in further work abbreviated as HA/SF) or HA-Tyr hydrogels. We demonstrated that all hydrogel formulations were cytocompatible and could promote the expression of cartilage matrix proteins allowing chondrocytes to produce ECM, while the most prominent chondrogenic effects were observed in hydrogels with HA20/SF80 polymeric ratios. Unconfined mechanical testing showed that the compressive modulus for HA20/SF80 chondrocyte-laden constructs was increased almost 10-fold over 28 days of culture in chondrogenic medium which confirmed the superior production of ECM in this hydrogel compared to other hydrogels in this study. Furthermore, in hydrogels loaded with anabolic and anti-inflammatory drugs, HA20/SF80 hydrogel showed the longest and the most sustained release profile over time which is desirable for the long treatment duration typically necessary for osteoarthritic joints. In conclusion, HA20/SF80 hydrogel was successfully established as a suitable injectable biomaterial for cartilage tissue engineering and drug delivery applications.},
  subject      = {Tissue Engineering},
  language  = {en}
}
@article{QuartinelloTallianAueretal.,
  author    = {Quartinello, Felice and Tallian, Claudia and Auer, Julia and Sch{\"o}n, Herta and Vielnascher, Robert and Weinberger, Simone and Wieland, Karin and Weihs, Anna and Rollett, Alexandra and Lendl, Bernhard and Teuschl, Andreas and Pellis, Alessandro and G{\"u}bitz, Georg},
  title     = {Smart Textiles in Wound Care: Functionalization of Cotton/PET Blends with Antimicrobial Nanocapsules},
  series = {Journal of Materials Chemistry B},
  journal   = {Journal of Materials Chemistry B},
  subject      = {Smart textiles},
  language  = {en}
}
@article{FeichtingerMonforteKeibletal.,
  author    = {Feichtinger, Xaver and Monforte, Xavier and Keibl, Claudia and Hercher, David and Schanda, Jakob and Teuschl, Andreas and Muschitz, Christian and Redl, Heinz and Fialka, Christian and Mittermayr, Rainer},
  title     = {Substantial Biomechanical Improvement by Extracorporeal Shockwave Therapy After Surgical Repair of Rodent Chronic Rotator Cuff Tears.},
  series = {American Journal of Sports Medicine},
  journal   = {American Journal of Sports Medicine},
  subject      = {Shockwave Therapy},
  language  = {en}
}
@article{TeuschlTanglHeimeletal.,
  author    = {Teuschl, Andreas and Tangl, Stefan and Heimel, Patrick and Schwarze, Uwe Yacine and Monforte, Xavier and Redl, Heinz and Nau, Thomas},
  title     = {Osteointegration of a Novel Silk Fiber-Based ACL Scaffold by Formation of a Ligament-Bone Interface.},
  series = {American Journal of Sports Medicine},
  journal   = {American Journal of Sports Medicine},
  subject      = {Tissue Engineering},
  language  = {en}
}
@article{SimsaMonforteSalzeretal.,
  author    = {Simsa, Robin and Monforte, Xavier and Salzer, Elias and Teuschl, Andreas and Jenndahl, Lachmi and Bergh, Niklas and Fogelstrand, Per},
  title     = {Effect of fluid dynamics on decellularization efficacy and mechanical properties of blood vessels.},
  series = {PLoS One},
  journal   = {PLoS One},
  subject      = {Tissue Engineering},
  language  = {en}
}
@article{JesenbergerZmajkovicovaCatalanottietal.,
  author    = {Jesenberger, Veronika and Zmajkovicova, Katarina and Catalanotti, Federica and Baumgartner, Christian and Reyes, Gloria Ximena and Baccarini, Manuela},
  title     = {MEK1 is required for PTEN membrane recruitment, AKT regulation, and the maintenance of peripheral tolerance},
  series = {Mol. Cell},
  volume    = {2013},
  journal   = {Mol. Cell},
  number    = {50},
  pages     = {43 -- 55},
  abstract  = {The Raf/MEK/ERK and PI3K/Akt pathways are prominent effectors of oncogenic Ras. These pathways negatively regulate each other, but the mechanism involved is incompletely understood. We now identify MEK1 as an essential regulator of lipid/protein phosphatase PTEN, through which it controls phosphatidylinositol-3-phosphate accumulation and AKT signaling. MEK1 ablation stabilizes AKT activation and, in vivo, causes a lupus-like autoimmune disease and myeloproliferation. Mechanistically, MEK1 is necessary for PTEN membrane recruitment as part of a ternary complex containing the multidomain adaptor MAGI1. Complex formation is independent of MEK1 kinase activity but requires phosphorylation of T292 on MEK1 by activated ERK. Thus, inhibiting the ERK pathway reduces PTEN membrane recruitment, increasing phosphatidylinositol-3-phosphate accumulation and AKT activation. Our data offer a conceptual framework for the observation that activation of the PI3K pathway frequently mediate resistance to MEK inhibitors and for the promising results obtained by combined MEK/PI3K inhibition in preclinical cancer models.},
  subject      = {MEK1 pathway},
  language  = {en}
}
@inproceedings{NebelSaladoManzanoJustetal.,
  author    = {Nebel, Sabrina and Salado Manzano, Cristina and Just, Valentin and Leeb, Christine and Jesenberger, Veronika},
  title     = {Role of the MEK/ERK pathway in chondrogenic differentiation: Establishment of a protocol for the generation of MEK1-knockout hTERT ASCs and assessment of their differentiation potential},
  series = {Proceedings des Forschungsforum 2017 der {\"o}sterreichischen Fachhochschulen},
  booktitle = {Proceedings des Forschungsforum 2017 der {\"o}sterreichischen Fachhochschulen},
  subject      = {Chondrogenesis},
  language  = {en}
}
@misc{NebelSaladoManzanoJustetal.,
  author    = {Nebel, Sabrina and Salado Manzano, Cristina and Just, Valentin and Leeb, Christine and Jesenberger, Veronika},
  title     = {Role of the MEK/ERK pathway in chondrogenic differentiation: Establishment of a protocol for the generation of MEK1-knockout hTERT ASCs and assessment of their differentiation potential},
  subject      = {Chondrogenesis},
  language  = {en}
}
@inproceedings{LeitnerGeppLiousiaetal.,
  author    = {Leitner, Rita and Gepp, Barbara and Liousia, Varvara and R{\"u}nzler, Dominik},
  title     = {Teaching 3R principles in ecotoxicology by performing a problem-based learning project},
  series = {ALTEX Proceedings, LINZ 2018 - EUSAAT 2018, Volume 7, No. 2, ISSN 2194-0479 (2018)},
  booktitle = {ALTEX Proceedings, LINZ 2018 - EUSAAT 2018, Volume 7, No. 2, ISSN 2194-0479 (2018)},
  subject      = {Problem Based Learning},
  language  = {en}
}
@article{CatalanottiReyesJesenbergeretal.,
  author    = {Catalanotti, Federica and Reyes, Gloria Ximena and Jesenberger, Veronika and Galabova-Kovacs, Gergana and de Matos Simoes, Ricardo and Carugo, Oliviero and Baccarini, Manuela},
  title     = {A Mek1-Mek2 heterodimer determines the strength and duration of the Erk signal},
  series = {Nat Struct Mol Biol.},
  volume    = {2009},
  journal   = {Nat Struct Mol Biol.},
  number    = {16(3)},
  pages     = {294 -- 303},
  abstract  = {Mek1 and Mek2 (also known as Map2k1 and Map2k2, respectively) are evolutionarily conserved, dual-specificity kinases that mediate Erk1 and Erk2 activation during adhesion and growth factor signaling. Here we describe a previously uncharacterized, unexpected role of Mek1 in downregulating Mek2-dependent Erk signaling. Mek1 mediates the regulation of Mek2 in the context of a previously undiscovered Mek1-Mek2 complex. The Mek heterodimer is negatively regulated by Erk-mediated phosphorylation of Mek1 on Thr292, a residue missing in Mek2. Disabling this Erk-proximal negative-feedback step stabilizes the phosphorylation of both Mek2 and Erk in cultured cells and in vivo in Mek1 knockout embryos and mice. Thus, in disagreement with the current perception of the pathway, the role of Mek1 and Mek2 in growth factor-induced Erk phosphorylation is not interchangeable. Our data establish Mek1 as the crucial modulator of Mek and Erk signaling and have potential implications for the role of Mek1 and Mek2 in tumorigenesis.},
  subject      = {Mek1-Mek2 pathway},
  language  = {en}
}
@misc{Leitner,
  author    = {Leitner, Rita},
  title     = {PBL-Szenario mit degressivem Support durch Lehrende im Modul "Angewandtes Forschungsprojekt in Umweltmanagement"},
  subject      = {Problem Based Learning},
  language  = {de}
}
@misc{GeppLiousiaMachaceketal.,
  author    = {Gepp, Barbara and Liousia, Varvara and Machacek, Thomas and Praher, Daniela and Eisner, Katharina and Limbeck, Sophie and Gamper, Janine and Schwarzl, Elisabeth and Hazod, Till and Landwehr, Renate and Wenger, Charlotte and Olscher, Christoph and Leitner, Rita and R{\"u}nzler, Dominik},
  title     = {Pelargonic acid - an alternative to glyphosate-based herbicides?},
  subject      = {Ecotoxicology},
  language  = {en}
}
@misc{Leitner,
  author    = {Leitner, Rita},
  title     = {Teaching 3R principles in ecotoxicology by performing a problem-based learning project},
  subject      = {Problem Based Learning},
  language  = {en}
}
@misc{KueenburgTeuschl,
  author    = {K{\"u}enburg, Bernhard and Teuschl, Andreas},
  title     = {Regenerative silk ligament: scale up and regulatory strategy of a textile engineered silk implant for tissue regeneration of injured human ACL (anterior cruciate ligament)},
  abstract  = {61 a93591 27-SY-1 Regenerative silk ligament: scale up and regulatory strategy of a textile engineered silk implant for tissue regeneration of injured human ACL (anterior cruciate ligament) Bernhard K{\"u}enburg，Andreas Teuschl MorphoMed, Austria In the USA around 370.000 annual ACL ruptures in the predominately young population (age 18-30) following sport injuries cause a lot of pain and long term troubles for the affected patients. The well established standard procedure of autologous tissue transplantation stabilizes the knee and allows sport activities after 9-12 months. However, harvest of autologous tissue such as patellar tendon or semitendinosus (the most frequently used autograft) weakens other body areas and long term data show an increased rate of arthroses (up to 50\% after 10 years) associated with ACL reconstructions. Based on numerous preclinical data and data from a 12-month sheep study the scale-up as well as regulatory approval process of a novel textile engineered silk implant as ACL graft have been initiated. It is planned to enter a clinical trial and strive for regulatory approval as a medical class 3 implant. The sheep data (1) have revealed an approximately 50\% degradation of the purified medical silk, which acts as scaffold for the regrowth of a new endogenous ligament. The formation of oriented collagenous tissue fibers including vascularization proves a ligament tissue regeneration for the first time. Scale-up and implementation of a commercial process require a defined set of specifications of commercial silkworm (Bombyx mori) silk, a biological raw material sourced from the textile industry as well as adequate analytical methods to characterize the depletion of sericin in the course of the production process. Based on first full scale samples, the defined biocompatibility program has to be executed, in the EU following the ISO requirements of the notified bodies, in the USA determined by the FDA. Upon submission of the full technical documentation as well as the biocompati-bility data, approval of a clinical trial can be achieved in order to demonstrate the clinical efficacy of the silk based ACL graft compared to the gold standard method in two different randomized groups. As primary endpoint the knee stability will be tested by an apparatus supported Lachmann test. In this study, besides the clinical benefit the patient safety is an important goal. The results of this study will be used for achievement of regulatory approval. References: 1) Teuschl A, Heimel P, N{\"u}rnberger S, van Griensven M, Redl H, Nau T. Am. J. Sports Med. 2016;44:1547-57. 2) Teuschl AH, van Griensven M, Redl H. Tissue Eng. Part C Methods. 2014;20:431-9.},
  subject      = {Biomaterial},
  language  = {en}
}
@article{TallianHerreroRollettStadleretal.,
  author    = {Tallian, Claudia and Herrero-Rollett, Alexandra and Stadler, Karina and Vielnascher, Robert and Wieland, Karin and Weihs, Anna and Pellis, Alessandro and Teuschl, Andreas and Lendl, Bernhard and Amenitsch, Heinz and Guebitz, Georg M.},
  title     = {Structural insights into pH-responsive drug release of self-assembling human serum albumin-silk fibroin nanocapsules.},
  series = {European Journal of Pharmaceutics and Biopharmaceutics},
  journal   = {European Journal of Pharmaceutics and Biopharmaceutics},
  abstract  = {Inflammation processes are associated with significant decreases in tissue or lysosomal pH from 7.4 to 4, a fact that argues for the application of pH-responsive drug delivery systems. However, for their design and optimization a full understanding of the release mechanism is crucial. In this study we investigated the pH-depending drug release mechanism and the influence of silk fibroin (SF) concentration and SF degradation degree of human serum albumin (HSA)-SF nanocapsules. Sonochemically produced nanocapsules were investigated regarding particle size, colloidal stability, protein encapsulation, thermal stability and drug loading properties. Particles of the monodisperse phase showed average hydrodynamic radii between 438 and 888 nm as measured by DLS and AFM and a zeta potential of -11.12 ± 3.27 mV. Together with DSC results this indicated the successful production of stable nanocapsules. ATR-FTIR analysis demonstrated that SF had a positive effect on particle formation and stability due to induced beta-sheet formation and enhanced crosslinking. The pH-responsive release was found to depend on the SF concentration. In in-vitro release studies, HSA-SF nanocapsules composed of 50\% SF showed an increased pH-responsive release for all tested model substances (Rhodamine B, Crystal Violet and Evans Blue) and methotrexate at the lowered pH of 4.5 to pH 5.4, while HSA capsules without SF did not show any pH-responsive drug release. Mechanistic studies using confocal laser scanning microscopy (CLSM) and small angle X-ray scattering (SAXS) analyses showed that increases in particle porosity and decreases in particle densities are directly linked to pH-responsive release properties. Therefore, the pH-responsive release mechanism was identified as diffusion controlled in a novel and unique approach by linking scattering results with in vitro studies. Finally, cytotoxicity studies using the human monocytic THP-1 cell line indicated non-toxic behavior of the drug loaded nanocapsules when applied in a concentration of 62.5 µg mL-1.},
  subject      = {Biomaterial},
  language  = {en}
}
@misc{TeuschlSchuhWeihsetal.,
  author    = {Teuschl, Andreas and Schuh, Christina and Weihs, Anna and Guillaume, Olivier and Monforte Vila, Xavier and Redl, Heinz and Kaplan, David and R{\"u}nzler, Dominik},
  title     = {Tailoring bioactivity of silk-based biomaterials via delivering and functionalization strategies with fibrinogen/thrombin, plant lectins or laminin},
  subject      = {Biomaterials},
  language  = {en}
}
@article{SlezakSlezakHartingeretal.,
  author    = {Slezak, Paul and Slezak, Cyrill and Hartinger, Joachim and Teuschl, Andreas and N{\"u}rnberger, Sylvia and Redl, Heinz and Mittermayr, Rainer},
  title     = {A Low Cost Implantation Model in the Rat That Allows a Spatial Assessment of Angiogenesis.},
  series = {Frontiers in Bioengineering and Biotechnology},
  journal   = {Frontiers in Bioengineering and Biotechnology},
  abstract  = {There is continual demand for animal models that allow a quantitative assessment of angiogenic properties of biomaterials, therapies, and pharmaceuticals. In its simplest form, this is done by subcutaneous material implantation and subsequent vessel counting which usually omits spatial data. We have refined an implantation model and paired it with a computational analytic routine which outputs not only vessel count but also vessel density, distribution, and vessel penetration depth, that relies on a centric vessel as a reference point. We have successfully validated our model by characterizing the angiogenic potential of a fibrin matrix in conjunction with recombinant human vascular endothelial growth factor (rhVEGF165). The inferior epigastric vascular pedicles of rats were sheathed with silicone tubes, which were subsequently filled with 0.2 ml of fibrin and different doses of rhVEGF165, centrically embedding the vessels. Over 4 weeks, tissue samples were harvested and subsequently immunohistologically stained and computationally analyzed. The model was able to detect variations over the angiogenic potentials of growth factor spiked fibrin matrices. Adding 20 ng of rhVEGF165 resulted in a significant increase in vasculature while 200 ng of rhVEGF165 did not improve vascular growth. Vascularized tissue volume increased during the first week and vascular density increased during the second week. Total vessel count increased significantly and exhibited a peak after 2 weeks which was followed by a resorption of vasculature by week 4. In summary, a simple implantation model to study in vivo vascularization with only a minimal workload attached was enhanced to include morphologic data of the emerging vascular tree.},
  subject      = {Tissue Engineering},
  language  = {en}
}
@article{BerkovitchCohenPeledetal.,
  author    = {Berkovitch, Yulia and Cohen, Talia and Peled, Eli and Schmidhammer, Robert and Hildner, Florian and Teuschl, Andreas and Wolbank, Susanne and Yelin, Dvir and Redl, Heinz and Seliktar, Dror},
  title     = {Hydrogel composition and laser micropatterning to regulate sciatic nerve regeneration.},
  series = {Journal of Tissue Engineering and Regenerative Medicine},
  journal   = {Journal of Tissue Engineering and Regenerative Medicine},
  pages     = {1049 -- 1061},
  abstract  = {Treatment of peripheral nerve injuries has evolved over the past several decades to include the use of sophisticated new materials endowed with trophic and topographical cues that are essential for in vivo nerve fibre regeneration. In this research, we explored the use of an advanced design strategy for peripheral nerve repair, using biological and semi-synthetic hydrogels that enable controlled environmental stimuli to regenerate neurons and glial cells in a rat sciatic nerve resection model. The provisional nerve growth conduits were composed of either natural fibrin or adducts of synthetic polyethylene glycol and fibrinogen or gelatin. A photo-patterning technique was further applied to these 3D hydrogel biomaterials, in the form of laser-ablated microchannels, to provide contact guidance for unidirectional growth following sciatic nerve injury. We tested the regeneration capacity of subcritical nerve gap injuries in rats treated with photo-patterned materials and compared these with injuries treated with unpatterned hydrogels, either stiff or compliant. Among the factors tested were shear modulus, biological composition, and micropatterning of the materials. The microchannel guidance patterns, combined with appropriately matched degradation and stiffness properties of the material, proved most essential for the uniform tissue propagation during the nerve regeneration process.},
  subject      = {Tissue Engineering},
  language  = {en}
}
@article{NauTeuschlEbneretal.,
  author    = {Nau, Thomas and Teuschl, Andreas and Ebner, Anna and Jung, Ilse and Schenk, Christian},
  title     = {Low revision rate and excellent outcome of primary ACL repair with a minimum follow-up of 5 years.},
  series = {Muscle, ligaments and tendons Journal},
  journal   = {Muscle, ligaments and tendons Journal},
  pages     = {185 -- 190},
  abstract  = {Introduction: Due to limitations of ACL reconstruction, primary ACL repair has recently regained research interest. Although abandoned in the past, primary repair with conservation of the original ligament demonstrates considerable advantages compared to reconstruction. We hypothesized that early repair, strictly limited to patients with a proximal ACL rupture and excellent tissue quality of the remaining ACL stump, would lead to equal revision rates and subjective outcomes as reported for ACL reconstruction after a minimum of 5 years. Methods: In this questionnaire study, patients who had a primary ACL repair between 2002 and 2009 were invited to participate. Besides any potential revision surgery, the Tegner activity scale and the Knee Injury and Osteoarthritis Outcome Score were included in the evaluation. Results: Out of 1912 patients who had ACL related surgery during the observation period, 221 (11.4\%) had a primary ACL repair. 60 patients (61 knees) were available for follow-up. In 2/61 (3.3\%) cases ACL revision surgery was performed and one patient had meniscus surgery of the affected side. The median Tegner activity scale was 6 (range, 3 to 10). The mean KOOS subscores were 88.8\% (Function/Sports), 86.6\% (Quality of life), 94.6 (Symptoms), 94.0 (Pain) and 97.0 (Activities of Daily Living). Conclusion: Primary ACL repair, strictly limited to proximal ruptures with good tissue quality leads to revision rates and subjective outcome comparable to ACL reconstruction. Level of evidence: IV.},
  subject      = {Regeneration},
  language  = {en}
}
@misc{TeuschlHeimelMonforteVilaetal.,
  author    = {Teuschl, Andreas and Heimel, Patrick and Monforte Vila, Xavier and N{\"u}rnberger, Sylvia and Tangl, Stefan and van Griensven, Martijn and Redl, Heinz and Nau, Thomas},
  title     = {Anterior cruciate ligament regeneration using the silk-based RegACL scaffold},
  subject      = {Tissue Engineering},
  language  = {en}
}
@incollection{HackethalSchuhHoferetal.,
  author    = {Hackethal, Johannes and Schuh, Christina and Hofer, Alexandra and Meixner, Barbara and Hennerbichler, Simone and Redl, Heinz and Teuschl, Andreas},
  title     = {Human Placenta Laminin-111 as a Multifunctional Protein for Tissue Engineering and Regenerative Medicine},
  series = {Advances in Experimental Medicine and Biology},
  booktitle = {Advances in Experimental Medicine and Biology},
  publisher = {Springer},
  publisher      = {Fachhochschule Technikum Wien},
  subject      = {Biomaterial},
  language  = {en}
}
@misc{SalzerRiederMonforteVilaetal.,
  author    = {Salzer, Elias and Rieder, Bernhard and Monforte Vila, Xavier and Weihs, Anna and R{\"u}nzler, Dominik and Teuschl, Andreas},
  title     = {Evaluation of a novel hydrostatic pressure bioreactor on bovine cartilage chips},
  subject      = {Bioreactor},
  language  = {en}
}
@misc{SlezakRoseHercheretal.,
  author    = {Slezak, Paul and Rose, Roland and Hercher, David and Weihs, Anna and Fuchs, Christiane and Redl, Heinz and Mittermayr, Rainer and Slezak, Cyrill},
  title     = {Tracking therapeutic shockwaves and their impact on regeneration},
  subject      = {Shockwave Therapy},
  language  = {en}
}
@misc{EisnerGeppSchneggetal.,
  author    = {Eisner, Katharina and Gepp, Barbara and Schnegg, Raimund and Dallinger, Reinhard and R{\"u}nzler, Dominik},
  title     = {Investigation of endocrine effects of sublethal cadmium doses on the reproduction system of the freshwater snail B. glabrata},
  subject      = {Ecotoxicology},
  language  = {en}
}
@misc{SalzerLiousiaMonforteVilaetal.,
  author    = {Salzer, Elias and Liousia, Varvara and Monforte Vila, Xavier and R{\"u}nzler, Dominik},
  title     = {Tracking of small aquatic organisms with custom-made tracking plates},
  subject      = {Ecotoxicology},
  language  = {en}
}
@article{HackethalMuehlederHoferetal.,
  author    = {Hackethal, Johannes and M{\"u}hleder, Severin and Hofer, Alexandra and Schneider, Karl Heinrich and Pr{\"u}ller, Johanna and Hennerbichler, Simone and Redl, Heinz and Teuschl, Andreas},
  title     = {An Effective Method of Atelocollagen Type 1/3 Isolation from Human Placenta and Its In Vitro Characterization in Two-Dimensional and Three-Dimensional Cell Culture Applications},
  series = {Tissue Eng Part C Methods},
  volume    = {23},
  journal   = {Tissue Eng Part C Methods},
  number    = {5},
  pages     = {274 -- 285},
  subject      = {Placenta},
  language  = {en}
}
@inproceedings{FuchsWeihsSzwarcetal.,
  author    = {Fuchs, Christiane and Weihs, Anna and Szwarc, Dorota and Mittermayr, Rainer and R{\"u}nzler, Dominik and Teuschl, Andreas},
  title     = {Shock wave treatment of muscle (stem) cells - a new implementation for regeneration},
  series = {Proceedings of the 20th International Congress of the ISMST},
  booktitle = {Proceedings of the 20th International Congress of the ISMST},
  subject      = {Shockwave treatment},
  language  = {en}
}
@techreport{Freistetter,
  author    = {Freistetter, Florian},
  title     = {The Power of Lauf},
  organization    = {ORF Sendung "Science Busters"},
  subject      = {Bioreactor},
  language  = {de}
}
@article{BerkovitchCohenPeledetal.,
  author    = {Berkovitch, Yulia and Cohen, Talia and Peled, Eli and Schmidhammer, Robert and Hildner, Florian and Teuschl, Andreas and Wolbank, Susanne and Yelin, Dvir and Redl, Heinz and Seliktar, Dror},
  title     = {Hydrogel Composition and Laser Micro-Patterning to Regulate Sciatic Nerve Regeneration},
  series = {Journal of Tissue Engineering and Regenerative Medicine},
  volume    = {12},
  journal   = {Journal of Tissue Engineering and Regenerative Medicine},
  number    = {4},
  subject      = {Micro-Patterning},
  language  = {en}
}
@misc{FuchsWeihsSzwarcetal.,
  author    = {Fuchs, Christiane and Weihs, Anna and Szwarc, Dorota and Mittermayr, Rainer and R{\"u}nzler, Dominik and Teuschl, Andreas},
  title     = {Shock wave treatment of muscle (stem) cells - a new implementation for regeneration},
  subject      = {Shockwave treatment},
  language  = {en}
}
@misc{TeuschlWeihsFuchsetal.,
  author    = {Teuschl, Andreas and Weihs, Anna and Fuchs, Christiane and Monforte Vila, Xavier},
  title     = {Silk as a versatile biomaterial for musculoskeletal tissue engineering},
  subject      = {Silk},
  language  = {en}
}
@misc{SzwarcFuchsWeihsetal.,
  author    = {Szwarc, Dorota and Fuchs, Christiane and Weihs, Anna and R{\"u}nzler, Dominik},
  title     = {Molecular mechanisms underlying the potential of shock wave treatment for cardiac therapy},
  subject      = {Shockwave treatment},
  language  = {en}
}
@misc{SzwarcFuchsPurtscheretal.,
  author    = {Szwarc, Dorota and Fuchs, Christiane and Purtscher, Michaela and R{\"u}nzler, Dominik},
  title     = {Elucidating the molecular mechanisms underlying cardiac shock wave therapy},
  subject      = {Shockwave treatment},
  language  = {en}
}
@article{BernhardFergusonRiederetal.,
  author    = {Bernhard, Jonathan and Ferguson, James and Rieder, Bernhard and Heimel, Patrick and Nau, Thomas and Tangl, Stefan and Redl, Heinz and Vunjak-Novakovic, Gordana},
  title     = {Tissue-engineered hypertrophic chondrocyte grafts enhanced long bone repair biomaterials},
  series = {Biomaterials},
  journal   = {Biomaterials},
  number    = {139},
  pages     = {202 -- 212},
  subject      = {Grafting},
  language  = {en}
}
@misc{Ruenzler,
  author    = {R{\"u}nzler, Dominik},
  title     = {Alternatives to Animal Experiment Models: Tracking Zebrafish Larvae and Daphnia},
  subject      = {Animal Experiments},
  language  = {en}
}
@misc{Fuchs,
  author    = {Fuchs, Christiane},
  title     = {The importance of mechanotransduction in myogenesis},
  subject      = {Mechanotransduction},
  language  = {en}
}
@article{SchneiderLehmannvanOschetal.,
  author    = {Schneider, Cornelia and Lehmann, Johannes and van Osch, Gerjo and Hildner, Florian and Teuschl, Andreas and Monforte Vila, Xavier and Miosga, David and Heimel, Patrick and Priglinger, Eleni and Redl, Heinz and Wolbank, Susanne and N{\"u}rnberger, Sylvia},
  title     = {Systematic Comparison of Protocols for the Preparation of Human Articular Cartilage for Use as Scaffold Material in Cartilage Tissue Engineering},
  series = {Tissue Eng Part C Methods},
  volume    = {22},
  journal   = {Tissue Eng Part C Methods},
  number    = {12},
  subject      = {Cartilage},
  language  = {en}
}
@inproceedings{MaleinerHeherTeuschletal.,
  author    = {Maleiner, Babette and Heher, Philipp and Teuschl, Andreas and Redl, Heinz and R{\"u}nzler, Dominik and Fuchs, Christiane},
  title     = {Generation of aligned skeletal muscle-like tissue based on the application of strain to a 3D fibrin scaffold},
  series = {Proceedings of PACT "Designer Cells go Clinical" Symposium},
  booktitle = {Proceedings of PACT "Designer Cells go Clinical" Symposium},
  subject      = {Tissue Generation},
  language  = {en}
}
@misc{SzwarcFuchsWeihsetal.,
  author    = {Szwarc, Dorota and Fuchs, Christiane and Weihs, Anna and Monforte Vila, Xavier and Hanetseder, Dominik and Teuschl, Andreas and R{\"u}nzler, Dominik},
  title     = {The effect of shock waves on in vitro cartilage development in silk scaffolds},
  subject      = {Shockwave treatment},
  language  = {en}
}
@misc{MaleinerHeherTeuschletal.,
  author    = {Maleiner, Babette and Heher, Philipp and Teuschl, Andreas and Redl, Heinz and R{\"u}nzler, Dominik and Fuchs, Christiane},
  title     = {Generation of aligned skeletal muscle-like tissue based on the application of strain to a 3D fibrin scaffold},
  subject      = {Tissue Generation},
  language  = {en}
}
@misc{TeuschlFuchsWeihsetal.,
  author    = {Teuschl, Andreas and Fuchs, Christiane and Weihs, Anna and Heimel, Patrick and R{\"u}nzler, Dominik and Redl, Heinz and Nau, Thomas},
  title     = {The Silk Road from Textiles to Novel Medical Implants},
  subject      = {Silk},
  language  = {en}
}
@misc{TeuschlHeimelNuernbergeretal.,
  author    = {Teuschl, Andreas and Heimel, Patrick and N{\"u}rnberger, Sylvia and Redl, Heinz and Nau, Thomas},
  title     = {ACL Regeneration using a novel silk fiber based scaffold - Histological Results of a Large Animal Study},
  subject      = {Tissue Regeneration},
  language  = {en}
}
@misc{Teuschl,
  author    = {Teuschl, Andreas},
  title     = {Silk Processing - from Gels to ACL Replacement},
  subject      = {Silk},
  language  = {en}
}
@inproceedings{FuchsSzwarcWeihsetal.,
  author    = {Fuchs, Christiane and Szwarc, Dorota and Weihs, Anna and R{\"u}nzler, Dominik},
  title     = {Shock wave treatment of 3D cardiac model systems activates ERK 1/2 signaling pathway and influences cardiomyogenesis},
  series = {Proceedings of PACT "Designer Cells go Clinical" Symposium},
  booktitle = {Proceedings of PACT "Designer Cells go Clinical" Symposium},
  subject      = {Shockwave treatment},
  language  = {en}
}
@inproceedings{FuchsSzwarcWeihsetal.,
  author    = {Fuchs, Christiane and Szwarc, Dorota and Weihs, Anna and R{\"u}nzler, Dominik},
  title     = {Shock wave treatment of 3D cardiac model systems activates ERK 1/2 signaling pathway and influences cardiomyogenesis},
  series = {Proceedings of LBG Meeting for Health Sciences 2016},
  booktitle = {Proceedings of LBG Meeting for Health Sciences 2016},
  subject      = {Shockwave treatment},
  language  = {en}
}
@misc{FuchsSzwarcWeihsetal.,
  author    = {Fuchs, Christiane and Szwarc, Dorota and Weihs, Anna and R{\"u}nzler, Dominik},
  title     = {Shock wave treatment of 3D cardiac model systems activates ERK 1/2 signaling pathway and influences cardiomyogenesis},
  subject      = {Shockwave treatment},
  language  = {en}
}
@inproceedings{FuchsSzwarcWeihsetal.,
  author    = {Fuchs, Christiane and Szwarc, Dorota and Weihs, Anna and R{\"u}nzler, Dominik},
  title     = {Shock wave treatment positively influences cardiomyogenesis in an energy-dependent manner},
  series = {Proceedings of PACT "Designer Cells go Clinical" Symposium},
  booktitle = {Proceedings of PACT "Designer Cells go Clinical" Symposium},
  subject      = {Shockwave treatment},
  language  = {en}
}
@misc{Weihs,
  author    = {Weihs, Anna},
  title     = {Shock wave treatment for in vitro tissue engineering applications},
  subject      = {Shockwave treatment},
  language  = {en}
}
@inproceedings{BasoliChaudryCrucianietal.,
  author    = {Basoli, Valentina and Chaudry, Sidrah and Cruciani, Sara and Fuchs, Christiane and Rieger, Sabine and Dungel, Peter and Wolbank, Susanne and Ventura, Carlo and Grillari-Voglauer, Regina and Redl, Heinz and Maioli, Margherita},
  title     = {Epigenetic and molecular behavious of stem cells exposed to biophysical stimuli: new insights in regenerative medicine},
  series = {Proceedings des Seminars zum 40. Jahresjubil{\"a}um der {\"O}sterreichischen Gesellschaft f{\"u}r Chirurgische Forschung},
  booktitle = {Proceedings des Seminars zum 40. Jahresjubil{\"a}um der {\"O}sterreichischen Gesellschaft f{\"u}r Chirurgische Forschung},
  subject      = {Stem Cells},
  language  = {en}
}
@misc{BasoliChaudryCrucianietal.,
  author    = {Basoli, Valentina and Chaudry, Sidrah and Cruciani, Gabriele and Fuchs, Christiane and Rieger, Sabine and Dungel, Peter and Wolbank, Susanne and Ventura, Carlo and Grillari-Voglauer, Regina and Redl, Heinz and Maioli, Margherita},
  title     = {Epigenetic and molecular behavious of stem cells exposed to biophysical stimuli: new insights in regenerative medicine},
  subject      = {Stem Cells},
  language  = {en}
}
@misc{HofstaetterBreyerKafkaetal.,
  author    = {Hofst{\"a}tter, Otto and Breyer, Peter and Kafka, Patricia and Sabo, Anton},
  title     = {Development of a prototype for the simulation of human sway to make standardized and reproducible measurements of force and pressure sensors},
  subject      = {Simulation},
  language  = {en}
}
@misc{HoettingerMallySabo,
  author    = {H{\"o}ttinger, Hannes and Mally, Franziska and Sabo, Anton},
  title     = {Activity recognition in surfing - a comparative study between hidden markov model and support vector machine},
  subject      = {Activity},
  language  = {de}
}
@misc{Weihs,
  author    = {Weihs, Anna},
  title     = {Shock wave treatment for in vitro tissue engineering applications},
  subject      = {Shockwave treatment},
  language  = {en}
}
@misc{Weihs,
  author    = {Weihs, Anna},
  title     = {Mechanotransduction in shock wave treatment},
  subject      = {Shockwave treatment},
  language  = {en}
}
@misc{MandtPichlerMelchioretal.,
  author    = {Mandt, Denise and Pichler, Verena and Melchior, Sophie and R{\"u}nzler, Dominik},
  title     = {Establishment of 2D and 3D cell culture assays using RT-gillW1 cells},
  subject      = {Cell Culture},
  language  = {en}
}
@misc{OettlMallySabo,
  author    = {{\"O}ttl, Georg and Mally, Franziska and Sabo, Anton},
  title     = {The influence of low-friction quickdraws on impact forces in climbing falls},
  subject      = {Climbing},
  language  = {en}
}
@misc{SalzerRuenzler,
  author    = {Salzer, Elias and R{\"u}nzler, Dominik},
  title     = {Acute Immobilization Test with Daphnia magna - A Refined Methodology for the Detection of Immobilization},
  subject      = {Immobilization},
  language  = {en}
}
@misc{SalzerRuenzler,
  author    = {Salzer, Elias and R{\"u}nzler, Dominik},
  title     = {Live or dead is not enough: a large-scale mobility assay with Daphnia magna},
  subject      = {Mobility Assay},
  language  = {en}
}
@misc{Fuchs,
  author    = {Fuchs, Christiane},
  title     = {A mechanical stimulation strategy for engineering skeletal muscle-like tissue on a strained fibrin scaffold},
  subject      = {Muscle Tissue},
  language  = {en}
}
@misc{Fuchs,
  author    = {Fuchs, Christiane},
  title     = {The beneficial effects of in vitro shock wave treatment on cardiomyogenesis are energy dependent},
  subject      = {In Vitro},
  language  = {en}
}
@misc{Fuchs,
  author    = {Fuchs, Christiane},
  title     = {Mechanotransduction - bioreactors},
  subject      = {Bioreactors},
  language  = {en}
}
@article{HeherMaleinerPruelleretal.,
  author    = {Heher, Philipp and Maleiner, Babette and Pr{\"u}ller, Johanna and Teuschl, Andreas and Kollmitzer, Josef and Monforte Vila, Xavier and Wolbank, Susanne and Redl, Heinz and R{\"u}nzler, Dominik and Fuchs, Christiane},
  title     = {A novel bioreactor for the generation of highly aligned 3D skeletal muscle-like constructs through orientation of fibrin via application of static strain},
  series = {Acta Biomaterialia},
  journal   = {Acta Biomaterialia},
  subject      = {Bioreactor},
  language  = {en}
}
@article{TeuschlSchuhHalbweisetal.,
  author    = {Teuschl, Andreas and Schuh, Christina and Halbweis, Robert and Pajer, Krisztian and Marton, Gabor and Hopf, Rudolf and Mosia, Shorena and R{\"u}nzler, Dominik and Redl, Heinz and Nogradi, Antal and Hausner, Thomas},
  title     = {A new preparation method for anisotropic silk fibroin nerve guidance conduits and its evaluation in vitro and in a rat sciatic nerve defect model},
  series = {Tissue Engineering Part C: Methods},
  journal   = {Tissue Engineering Part C: Methods},
  subject      = {Fibrin},
  language  = {en}
}
@misc{TeuschlSchuhHalbweisetal.,
  author    = {Teuschl, Andreas and Schuh, Christina and Halbweis, Robert and Marton, G{\´a}bor and Pajer, Kriszti{\´a}n and Hopf, Rudolf and Mosia, Shorena and R{\"u}nzler, Dominik and N{\´o}gr{\´a}di, Antal and Hausner, Thomas and Redl, Heinz},
  title     = {Silk fibroin for peripheral nerve regeneration: a novel preparation method improved mechanical characteristics and supports regeneration in rat sciatic nerves},
  subject      = {Fibroin},
  language  = {en}
}
@misc{FuchsWeihsTeuschletal.,
  author    = {Fuchs, Christiane and Weihs, Anna and Teuschl, Andreas and Hartinger, Joachim and Slezak, Paul and Mittermayr, Rainer and Redl, Heinz and Junger, Wolfgang and Sitte, Harald and R{\"u}nzler, Dominik},
  title     = {Shockwave Treatment Enhances Proliferation and Improves Wound Healing via Purinergic Signaling Linked ERK 1/2 Pathways},
  subject      = {Shockwave treatment},
  language  = {en}
}
@misc{FuchsWeihsTeuschletal.,
  author    = {Fuchs, Christiane and Weihs, Anna and Teuschl, Andreas and Hartinger, Joachim and Slezak, Paul and Mittermayr, Rainer and Redl, Heinz and Junger, Wolfgang and Sitte, Harald and R{\"u}nzler, Dominik},
  title     = {Shockwave Treatment Augments Proliferation and Improves Wound Healing via Purinergic Signaling Linked ERK 1/2 Pathways},
  subject      = {Shockwave treatment},
  language  = {en}
}
@misc{HeherFuchsPruelleretal.,
  author    = {Heher, Philipp and Fuchs, Christiane and Pr{\"u}ller, Johanna and Babette, Maleiner and R{\"u}nzler, Dominik and Redl, Heinz},
  title     = {A novel bioreactor for engineering skeletal muscle-like tissue in a strained fibrin scaffold},
  subject      = {Bioreactor},
  language  = {en}
}
@article{GuillaumeParkMonforteVilaetal.,
  author    = {Guillaume, Olivier and Park, Jaesung and Monforte Vila, Xavier and Gruber-Blum, Simone and Redl, Heinz and Petter-Puchner, Akexander and Teuschl, Andreas},
  title     = {Fabrication of silk mesh with enhanced cytocompatibility: preliminary in vitro investigation toward cell-based therapy for hernia repair},
  series = {Journal of Materials Science: Materials in Medicine},
  journal   = {Journal of Materials Science: Materials in Medicine},
  subject      = {Cytocompatibility},
  language  = {en}
}
@misc{WeihsFuchsTeuschletal.,
  author    = {Weihs, Anna and Fuchs, Christiane and Teuschl, Andreas and Hartinger, Joachim and Slezak, Paul and Mittermayr, Rainer and Redl, Heinz and Junger, Wolfgang and Sitte, Harald and R{\"u}nzler, Dominik},
  title     = {Shockwave treatment activates Erk1/2 pathways predominantly via P2Y receptor involvement},
  subject      = {Shockwave},
  language  = {en}
}
@article{TeuschlHeimelNuernbergeretal.,
  author    = {Teuschl, Andreas and Heimel, Patrick and N{\"u}rnberger, Sylvia and van Griensven, Martijn and Redl, Heinz and Nau, Thomas},
  title     = {A Novel Silk Fiber-Based Scaffold for Regeneration of the Anterior Cruciate Ligament: Histological Results From a Study in Sheep.},
  series = {The American Journal of Sports Medicine},
  journal   = {The American Journal of Sports Medicine},
  subject      = {Ligament},
  language  = {en}
}
@misc{TeuschlFuchsFeichtingeretal.,
  author    = {Teuschl, Andreas and Fuchs, Christiane and Feichtinger, Georg and Heher, Philipp and Heimel, Patrick and Schuh, Christina and N{\"u}rnberger, Sylvia and Nau, Thomas and R{\"u}nzler, Dominik and Redl, Heinz},
  title     = {Fibrin or Fibroin - Not only the "o" Makes the Difference},
  subject      = {Fibrin},
  language  = {en}
}
@article{TeuschlBalmayorRedletal.,
  author    = {Teuschl, Andreas and Balmayor, Elizabeth and Redl, Heinz and van Griensven, Martijn and Dungel, Peter},
  title     = {Phototherapy With LED Light Modulates Healing Processes in an In Vitro Scratch Wound-Model Using 3 Different Cell Types},
  series = {Dermatologic Surgery},
  volume    = {41},
  journal   = {Dermatologic Surgery},
  number    = {2},
  pages     = {261 -- 268},
  subject      = {Phototherapy},
  language  = {en}
}
@article{SchuhHercherStaineretal.,
  author    = {Schuh, Christina and Hercher, David and Stainer, Michaela and Hopf, Rudolf and Teuschl, Andreas and Schmidhammer, Robert and Redl, Heinz},
  title     = {Extracorporeal shockwave treatment: A novel tool to improve Schwann cell isolation and culture},
  series = {Cytotherapy},
  journal   = {Cytotherapy},
  subject      = {Extracorporeal shockwave},
  language  = {en}
}
@misc{TeuschlNuernbergerHeimeletal.,
  author    = {Teuschl, Andreas and N{\"u}rnberger, Sylvia and Heimel, Patrick and Redl, Heinz and Nau, Thomas},
  title     = {Regeneration of the Anterior Cruciate Ligament Using a Silk-Fiber Based Scaffold - Histological Results},
  subject      = {Tissue Regeneration},
  language  = {en}
}
@article{NauRedlTeuschl,
  author    = {Nau, Thomas and Redl, Heinz and Teuschl, Andreas},
  title     = {Comment on: In Vivo Evaluation of Electrospun Polycaprolactone Graft for Anterior Cruciate Ligament Engineering},
  series = {Tissue Engineering Part A},
  journal   = {Tissue Engineering Part A},
  subject      = {Grafting},
  language  = {en}
}
@article{GauillaumeTeuschlGruberBlum,
  author    = {Gauillaume, Oliver and Teuschl, Andreas and Gruber-Blum, Simone},
  title     = {Emerging Trends in Abdominal Wall Reinforcement: Bringing Bio-Functionality to Meshes},
  series = {Advanced Healthcare Materials},
  journal   = {Advanced Healthcare Materials},
  subject      = {Abdominal Wall Reinforcement},
  language  = {en}
}
@article{NauTeuschl,
  author    = {Nau, Thomas and Teuschl, Andreas},
  title     = {Regeneration of the anterior cruciate ligament: Current strategies in tissue engineering},
  series = {World Journal of Orthopedics},
  volume    = {6},
  journal   = {World Journal of Orthopedics},
  number    = {1},
  subject      = {Ligament},
  language  = {en}
}
@phdthesis{Weihs,
  author    = {Weihs, Anna},
  title     = {Elucidation of extracorporeal shock wave treatment triggered intracellular processes},
  school      = {Fachhochschule Technikum Wien},
  abstract  = {Die Wirkung von Stoßwellen auf den menschlichen K{\"o}rper ist bereits seit Jahrzehnten bekannt. Ihr zerst{\"o}rerisches Potential wird seit den 1980-ern zur Behandlung und Desintegration von Nierensteinen angewendet, wo die Stoßwellentherapie heutzutage die Standardbehandlung darstellt. Der erste unerwartete Effekt der Therapie - die Verdichtung des Beckenkammes - wurde schon damals bei Folgeuntersuchungen von Patienten mit Nierensteinen festgestellt. Die Beobachtung, dass Stoßwellen Effekte auf Knochen hervorrufen, ebnete den Weg f{\"u}r die Anwendung der Stoßwellentherapie in Bereichen abseits der Urologie. Mittlerweile wird die Stoßwellentherapie nicht nur zur Behandlung von Knochenbruchheilungsst{\"o}rungen und Tendinopathien, sondern auch bei Weichteilwundheilungsst{\"o}rungen eingesetzt. Trotz der vielf{\"a}ltigen Anwendungsgebiete ist der zugrunde liegende Wirkungsmechanismus des positiven Effekts der Stoßwellentherapie bis heute noch nicht vollst{\"a}ndig aufgekl{\"a}rt. Die Aktivierung von mechanotransduktiven Signalwegen wurde bereits teilweise in vitro und in vivo gezeigt, jedoch meist bei kn{\"o}chernen Indikationen. Doch auch f{\"u}r die Anwendung der Stoßwellentherapie in der (verz{\"o}gerten) Wundheilung ist die Aufkl{\"a}rung des Wirkungsmechanismus essentiell. Erst dadurch k{\"o}nnte diese nicht- invasive, effiziente und großteils Nebenwirkungs-freie Therapieform auch in diesem Bereich als m{\"o}gliche Standardtherapieform genutzt werden. In dieser Dissertation wurde zuerst ein Set-up zur in vitro Stoßwellenanwendung optimiert. Mithilfe eines Molek{\"u}l-Aufnahme Assays wurden die technischen Parameter f{\"u}r die in vitro Stoßwellenbehandlung festgelegt. Mit diesem in vitro Aufbau sollten daraufhin jene intrazellul{\"a}ren Mechanismen identifiziert werden, die von der Stoßwelle beeinflusst werden. In einem in vivo Modell sollte schlussendlich die Rolle dieser Mechanismen am wundheilungsf{\"o}rdernden Effekt der Stoßwelle gekl{\"a}rt werden. Um m{\"o}glichst universelle Effekte der Stoßwelle auf intrazellul{\"a}re Signalwege zu untersuchen, wurden verschiedenste Zelllinien verwendet. Diese beinhalteten die humane U937 Monozyten Zelllinie, humane Jurkat T-Zellen, die humane MG63 Osteosarcoma Zelllinie, die murine C3H10T1/2 mesenchymale Progenitor Zelllinie sowie prim{\"a}re humane mononukle{\"a}re Zellen des peripheren Blutes. Die in den proliferativen Effekt der Stowellentherapie involvierte Signalkaskade konnte darauffolgend erstmals sowohl in murinen C3H10T1/2 Zellen als auch in humanen Fettstammzellen (adipose tissue-derived stem cells) und in humanen Jurkat T-Zellen detailliert beschrieben werden. Außerdem wurde ATP als entscheidendes Signalmolek{\"u}l identifiziert, welches nach Freisetzung durch Stoßwellenbehandlung mittels purinergem Signaling die Erk1/2 Signalkaskade aktiviert. F{\"u}r die dadurch gesteigerte Proliferation in stoßwellenbehandelten Zellen ist die Erk1/2 Aktivierung essentiell. In einem in vivo Model f{\"u}r gest{\"o}rte Wundheilung in der Ratte wurde die Hypothese zur entscheidenden Rolle des Erk1/2 Signalweges im wundheilungsf{\"o}rdernden Effekt der Stoßwelle best{\"a}tigt. In dieser Dissertation wurde gezeigt, dass die durch purinerges Signaling aktivierte Erk1/2 Signalkaskade eine entscheidende Rolle in der durch Stoßwellentherapie beschleunigten Zellproliferation in vitro und Wundheilung in vivo {\"u}bernimmt. Das damit erweiterte Verst{\"a}ndnis der Wirkungsmechanismen der Stoßwellentherapie kann zur Weiterentwicklung dieser Behandlungsform als zuk{\"u}nftige Standardtherapie bei Wundheilungsst{\"o}rungen, wie z.B. f{\"u}r diabetische oder chronische Wunden, beitragen.},
  subject      = {Healing},
  language  = {en}
}
@article{RosserCalvoSchlageretal.,
  author    = {Rosser, Julie and Calvo, Isabel Olmos and Schlager, Magdalena and Purtscher, Michaela and Jenner, Florien and Ertl, Peter},
  title     = {Recent Advances of Biologically Inspired 3D Microfluidic Hydrogel Cell Culture Systems},
  series = {J Cell Biol Cell Metalab},
  journal   = {J Cell Biol Cell Metalab},
  number    = {2},
  subject      = {Hydrogel},
  language  = {en}
}
@misc{Weihs,
  author    = {Weihs, Anna},
  title     = {Aufkl{\"a}rung des Wirkungsmechanismus der Stoßwellentherapie in der Wundheilung},
  subject      = {Shockwave},
  language  = {de}
}
@article{WeihsFuchsTeuschletal.,
  author    = {Weihs, Anna and Fuchs, Christiane and Teuschl, Andreas and Hartinger, Joachim and Slezak, Paul and Mittermayr, Rainer and Redl, Heinz and Junger, Wolfgang and Sitte, Harald and R{\"u}nzler, Dominik},
  title     = {Shock Wave Treatment Enhances Cell Proliferation and Improves Wound Healing by ATP Release-coupled Extracellular Signal-regulated Kinase (ERK) Activation},
  series = {The Journal of biological chemistry},
  journal   = {The Journal of biological chemistry},
  subject      = {Shockwave},
  language  = {de}
}
@article{SchuhHeherWeihsetal.,
  author    = {Schuh, Christina and Heher, Philipp and Weihs, Anna and Asmita, Banerjee and Wolbank, Susanne and Mittermayr, Rainer and Redl, Heinz and R{\"u}nzler, Dominik and Teuschl, Andreas},
  title     = {Adipose derived stem cells respond to in vitro extracorporeal shockwave treatment with increased stemness and multipotency},
  series = {New Biotechnology},
  journal   = {New Biotechnology},
  subject      = {Shockwave},
  language  = {en}
}
@misc{KrollerKreuzingerRuenzler,
  author    = {Kroller, Theophil and Kreuzinger, Norbert and R{\"u}nzler, Dominik},
  title     = {Validierung und Anwendung eines zellkultur-basierten Assays f{\"u}r Screening von endokrin wirksamen Stoffen in Abwasser},
  subject      = {Assay},
  language  = {de}
}
@misc{GuillaumeMonforteVila,
  author    = {Guillaume, Olivier and Monforte Vila, Xavier},
  title     = {Development of a Biodegradable Silk Mesh Suitable for Intra-Operative Fast Cell Seeding},
  subject      = {Silk},
  language  = {en}
}
@misc{Fuchs,
  author    = {Fuchs, Christiane},
  title     = {Shockwave treatment promotes proliferation via purinergic signaling coupled ERK1/2 pathway activation},
  subject      = {Shockwave},
  language  = {en}
}
@misc{HeherFuchsPruelleretal.,
  author    = {Heher, Philipp and Fuchs, Christiane and Pr{\"u}ller, Johanna and Maleiner, Babette and Kollmitzer, Josef and R{\"u}nzler, Dominik and Teuschl, Andreas and Wolbank, Susanne and Redl, Heinz},
  title     = {A bioreactor-based 3D culture system for skeletal muscle engineering in fibrin scaffolds},
  subject      = {Bioreactors},
  language  = {en}
}
@article{BanerjeeNuernbergerHennerbichleretal.,
  author    = {Banerjee, Asmita and N{\"u}rnberger, Sylvia and Hennerbichler, Simone and Riedl, Stefan and Schuh, Christina and Hacobian, Ara and Teuschl, Andreas and Eibl, J{\"u}rgen and Redl, Heinz},
  title     = {In toto differentiation of human amniotic membrane towards the Schwann cell lineage},
  series = {227-239},
  volume    = {15},
  journal   = {227-239},
  number    = {2},
  subject      = {Membrane},
  language  = {en}
}
@article{TeuschlNeutschMonforteVilaetal.,
  author    = {Teuschl, Andreas and Neutsch, Lukas and Monforte Vila, Xavier and R{\"u}nzler, Dominik and van Griensven, Martijn and Gabor, Franz and Redl, Heinz},
  title     = {Enhanced cell adhesion on silk fibroin via lectin surface modification.},
  series = {Acta Biomaterialia},
  journal   = {Acta Biomaterialia},
  subject      = {Silk},
  language  = {en}
}
@misc{Weihs,
  author    = {Weihs, Anna},
  title     = {Revealing underlying mechanisms of shock wave treatment induced wound healing},
  subject      = {Shockwave},
  language  = {en}
}
@article{ButaDavidDresseletal.,
  author    = {Buta, Christiane and David, Robert and Dressel, Ralf and Emgard, Mia and Fuchs, Christiane and Gross, Ulrike and Healy, Lyn and Hescheler, J{\"u}rgen and Kolar, Roman and Martin, Ulrich and Mikkers, Harald and M{\"u}ller, Franz-Josef and Schneider, Rebekka and Seiler, Andrea and Spielmann, Horst and Weitzer, Georg},
  title     = {REconsidering pluripotency tests: do we still need teratoma assays?},
  series = {Stem Cell Research},
  volume    = {11},
  journal   = {Stem Cell Research},
  number    = {1},
  pages     = {552 -- 562},
  subject      = {Assay},
  language  = {en}
}
@article{HohlriederTeuschlCichaetal.,
  author    = {Hohlrieder, Manfred and Teuschl, Andreas and Cicha, Klaus and van Griensven, Martijn and Redl, Heinz and Stampfl, J{\"u}rgen},
  title     = {Bioreactor and scaffold design for the mechanical stimulation of anterior cruciate ligament grafts},
  series = {Biomedical materials and engineering},
  volume    = {23},
  journal   = {Biomedical materials and engineering},
  number    = {3},
  pages     = {225 -- 237},
  subject      = {Bioreactors},
  language  = {en}
}
@article{DungelTeuschlBanerjeeetal.,
  author    = {Dungel, Peter and Teuschl, Andreas and Banerjee, Asmita and Paier-Pourani, Jamile and Redl, Heinz and Kozlov, Andrey},
  title     = {Impact of mitochondria on nitrite metabolism in HL-1 cardiomyocytes},
  series = {Frontiers in Physiology},
  journal   = {Frontiers in Physiology},
  number    = {4},
  subject      = {Nitrite},
  language  = {en}
}
@article{TeuschlNuernbergerRedletal.,
  author    = {Teuschl, Andreas and N{\"u}rnberger, Sylvia and Redl, Heinz and Nau, Thomas},
  title     = {Articular cartilage tissue regeneration: current research strategies and outlook for the future},
  series = {European Surgery},
  volume    = {45},
  journal   = {European Surgery},
  number    = {3},
  pages     = {142 -- 153},
  subject      = {Tissue Regeneration},
  language  = {en}
}
@article{TeuschlvanGriensvenRedl,
  author    = {Teuschl, Andreas and van Griensven, Martijn and Redl, Heinz},
  title     = {Sericin removal from raw Bombys mori silk scaffolds of high hierarchical order},
  series = {Tissue Eng Part C Methods},
  journal   = {Tissue Eng Part C Methods},
  subject      = {Scaffold},
  language  = {en}
}
@article{SchuhBanerjeeMosiaetal.,
  author    = {Schuh, Christina and Banerjee, Asmita and Mosia, Shorena and Hopf, Rudolf and Grasl, Christian and Schima, Heinrich and Schmidhammer, Robert and Redl, Heinz and R{\"u}nzler, Dominik and Morton, Tatjana J.},
  title     = {Activated Schwann-like cells guided by fibrin structures enhance Axonal Regeneration},
  series = {JOURNAL OF TISSUE ENGINEERING AND REGENERATIVE MEDICINE},
  volume    = {2012},
  journal   = {JOURNAL OF TISSUE ENGINEERING AND REGENERATIVE MEDICINE},
  number    = {vol. 6/no. 1},
  subject      = {Cells},
  language  = {en}
}
@article{RiederWeihsTeuschletal.,
  author    = {Rieder, Bernhard and Weihs, Anna and Teuschl, Andreas and Knebl, Gerald and Kollmitzer, Josef and Redl, Heinz and R{\"u}nzler, Dominik},
  title     = {Evaluation of cell response on permanent and pulsed atmospheric pressure stressed cells},
  series = {Journal of Tissue Engineering and Regenerative Medicine},
  volume    = {1},
  journal   = {Journal of Tissue Engineering and Regenerative Medicine},
  number    = {6},
  pages     = {240 -- 240},
  subject      = {Cells},
  language  = {en}
}
@article{WeihsJungerSchadenetal.,
  author    = {Weihs, Anna and Junger, Wolfgang and Schaden, Wolfgang and Sitte, Harald and R{\"u}nzler, Dominik},
  title     = {Extracorporeal shockwave treatment induced extracellular ATP release - a potential mechanism to activate wound healing},
  series = {Journal of Tissue Engineering and Regenerative Medicine},
  volume    = {1},
  journal   = {Journal of Tissue Engineering and Regenerative Medicine},
  number    = {6},
  pages     = {381 -- 381},
  subject      = {Shockwave treatment},
  language  = {en}
}
@article{RuenzlerWeihsJungeretal.,
  author    = {R{\"u}nzler, Dominik and Weihs, Anna and Junger, Wolfgang and Sitte, Harald and Schaden, Wolfgang},
  title     = {Extracorporeal shock waves - a novel method of mechanostimulation in tissue regeneration},
  series = {Journal of Tissue Engineering and Regenerative Medicine},
  volume    = {1},
  journal   = {Journal of Tissue Engineering and Regenerative Medicine},
  number    = {6},
  pages     = {380 -- 380},
  subject      = {Shockwave treatment},
  language  = {en}
}
@article{MartinMKollmitzerRedletal.,
  author    = {Martin M., Frank and Kollmitzer, Josef and Redl, Heinz and R{\"u}nzler, Dominik},
  title     = {Shear force stimulation of adipose-tissue derived stem cells in a novel bioreactor},
  series = {Journal of Tissue Engineering and Regenerative Medicine},
  volume    = {1},
  journal   = {Journal of Tissue Engineering and Regenerative Medicine},
  number    = {6},
  pages     = {340 -- 340},
  subject      = {Stem Cells},
  language  = {en}
}
@misc{WeihsJungerSchadenetal.,
  author    = {Weihs, Anna and Junger, Wolgang G. and Schaden, Wolfgang and Sitte, Harald H. and R{\"u}nzler, Dominik},
  title     = {Extracellular ATP release - a potential benefit in shock wave treatment},
  subject      = {Shockwave},
  language  = {en}
}
@article{MilanLoboGsandtnerGaubitzeretal.,
  author    = {Milan-Lobo, Laura and Gsandtner, Ingrid and Gaubitzer, Erwin and R{\"u}nzler, Dominik and Buchmayer, Florian and K{\"o}hler, Gottfried and Bonci, Antonello and Freissmuth, Michael and Sitte, Harald H.},
  title     = {Subtype-specific differences in corticotropin-releasing factor receptor complexes detected by fluorescence spectroscopy},
  series = {Molecular Pharmacology (mol pharmacol)},
  journal   = {Molecular Pharmacology (mol pharmacol)},
  number    = {76(6)},
  pages     = {1196 -- 1210},
  subject      = {Spectroscopy},
  language  = {en}
}
@inproceedings{WeihsKneblRedletal.,
  author    = {Weihs, Anna and Knebl, Gerald and Redl, Heinz and R{\"u}nzler, Dominik},
  title     = {Evaluation of cell migration methods in 3D hydrogels for tissue engineering applications},
  series = {3. Forschungsforum der {\"o}sterreichischen Fachhochschulen / Fachhochschule K{\"a}rnten},
  booktitle = {3. Forschungsforum der {\"o}sterreichischen Fachhochschulen / Fachhochschule K{\"a}rnten},
  isbn      = {978-3-853912850},
  pages     = {490 -- 491},
  subject      = {Cells},
  language  = {en}
}
@misc{Weihs,
  author    = {Weihs, Anna},
  title     = {Establishment of an in vitro experimental set-up to evaluate the biological effects of extracorporeal shock wave treatment},
  subject      = {Shockwave},
  language  = {en}
}
@inproceedings{KneblWeihsWeingantetal.,
  author    = {Knebl, Gerald and Weihs, Anna and Weingant, Michaela and Steininger, Thomas and Redl, Heinz and R{\"u}nzler, Dominik},
  title     = {Automatisierte Datenauswertung eines Boyden-Mikro-Chemotaxis-Kammer Zell-Migrations Assays.},
  series = {2. Forschungsforum der {\"o}sterreichischen Fachhochschulen},
  booktitle = {2. Forschungsforum der {\"o}sterreichischen Fachhochschulen},
  isbn      = {978-3-8322-7023-0},
  pages     = {412 -- 417},
  subject      = {Cells},
  language  = {de}
}
@inproceedings{RuenzlerKneblPeterbaueretal.,
  author    = {R{\"u}nzler, Dominik and Knebl, Gerald and Peterbauer, A. and Wolbank, Susanne and Morton, Tatjana J. and Redl, Heinz},
  title     = {Darstellung fluoreszenzmarkierter adulter Stammzellen in drei-dimensionaler Zellkultur mittels Konfokaler Laser Scanning Mikroskopie},
  series = {Erstes Forschungsforum der {\"o}sterreichischen Fachhochschulen},
  booktitle = {Erstes Forschungsforum der {\"o}sterreichischen Fachhochschulen},
  pages     = {311 -- 316},
  subject      = {Cells},
  language  = {en}
}
@article{GruntTomekWagneretal.,
  author    = {Grunt, Thomas and Tomek, Katharina and Wagner, Renate and Puckmair, Klaudia and Kainz, Birgit and R{\"u}nzler, Dominik and Gaiger, Alexander and K{\"o}hler, Gottfried and Zielinski, Christoph},
  title     = {Upregulation of retinoic acid receptor-beta by the epidermal growth factor-receptor inhibitor PD153035 is not mediated by blockade of ErbB pathways},
  series = {Journal of Cell Physiology},
  journal   = {Journal of Cell Physiology},
  number    = {211(3)},
  pages     = {803 -- 815},
  subject      = {Cells},
  language  = {en}
}
@article{WillerroiderFuchsBallmerWeberetal.,
  author    = {Willerroider, Marika and Fuchs, Heidemarie and Ballmer-Weber, Barbara and Focke, Margarete and Susani, M. and Thalhammer, J.},
  title     = {Cloning and molecular and immunological characterisation of two new food allergens, Cap a 2 and Lyc e 1, profilins from bell pepper (Capsicum annuum) and tomato (Lycopersicon esculentum)},
  series = {International Archives of Allergy and Immunology},
  volume    = {131},
  journal   = {International Archives of Allergy and Immunology},
  number    = {4},
  pages     = {245 -- 255},
  subject      = {Cloning},
  language  = {en}
}
@article{SpurnyAbdoulrahmanJandaetal.,
  author    = {Spurny, Radovan and Abdoulrahman, Kamaran and Janda, Lubomir and R{\"u}nzler, Dominik and K{\"o}hler, Gottfried and Castanon, Maria J. and Wiche, Gerhard},
  title     = {Oxidation and nitrosylation of cysteines proximal to the intermediate filament (IF)-binding site of plectin: effects on structure and vimentin binding and involvement in IF collapse},
  series = {Journal of Biological Chemistry},
  journal   = {Journal of Biological Chemistry},
  number    = {282(11)},
  pages     = {8175 -- 8187},
  subject      = {Oxidation},
  language  = {en}
}
@misc{Ruenzler,
  author    = {R{\"u}nzler, Dominik},
  title     = {Darstellung fluoreszenzmarkierter adulter Stammzellen in drei-dimensionaler Zellkultur mittels Konfokaler Laser Scanning Mikroskopie},
  subject      = {Cells},
  language  = {de}
}
@misc{Ruenzler,
  author    = {R{\"u}nzler, Dominik},
  title     = {Automatisierte Datenauswertung eines Boyden-Mikro-Chemotaxis-Kammer Zell-Migrations Assays},
  subject      = {Cells},
  language  = {en}
}
@inproceedings{MilanLoboRuenzlerGaubitzeretal.,
  author    = {Milan-Lobo, Laura and R{\"u}nzler, Dominik and Gaubitzer, Erwin and K{\"o}hler, Gottfried and Bonci, Antonello and Freissmuth, Michael and Sitte, Harald H.},
  title     = {CRF receptors form dynamic complexes at the plasma membrane but only type 2 receptor increases lateral mobility after CRF binding},
  series = {FEBS Journal},
  booktitle = {FEBS Journal},
  subject      = {Plasma Membrane},
  language  = {en}
}
@inproceedings{KaislerPazmandiOrtneretal.,
  author    = {Kaisler, Raphaela and P{\´a}zm{\´a}ndi, Christian and Ortner, Anna and Haberl, Ines and K{\"o}hler, Gottfried and R{\"u}nzler, Dominik and Artl, Andreas and Andreae, Fritz and Mosg{\"o}ller, Wilhelm},
  title     = {Vasoactive intestinal peptide conjugate internalisation as strategy for cytoplasmic drug delivery},
  series = {Journal of Molecular Neuroscience},
  booktitle = {Journal of Molecular Neuroscience},
  subject      = {Cytplasmic Drug Delivery},
  language  = {en}
}
@article{WrussRuenzlerSteigeretal.,
  author    = {Wruss, J{\"u}rgen and R{\"u}nzler, Dominik and Steiger, Christina and Chiba, Peter and K{\"o}hler, Gottfried and Blaas, Dieter},
  title     = {Attachment of VLDL receptors to an icosahedral virus along the 5-fold symmetry axis: multiple binding modes evidenced by fluorescence correlation spectroscopy},
  series = {Biochemistry},
  journal   = {Biochemistry},
  number    = {46(21)},
  pages     = {6331 -- 6339},
  subject      = {Cells},
  language  = {en}
}
@article{MaierRuenzlerSchindelaretal.,
  author    = {Maier, Christina and R{\"u}nzler, Dominik and Schindelar, Julia and Grabner, Gottfried and Waldh{\"a}usl, Werner Klaus and K{\"o}hler, Gottfried and Luger, Anton},
  title     = {G-protein-coupled glucocorticoid receptors on the pituitary cell membrane},
  series = {Journal of Cell Science},
  journal   = {Journal of Cell Science},
  number    = {118(15)},
  pages     = {3353 -- 3361},
  subject      = {Cells},
  language  = {en}
}
@article{MaierRuenzlerWagneretal.,
  author    = {Maier, Christina and R{\"u}nzler, Dominik and Wagner, Ludwig and Grabner, G{\"u}nther and K{\"o}hler, Gottfried and Lugner, Anton},
  title     = {Evidence for specific glucocorticoid binding sites on the cell membrane by fluorescence correlation spectroscopy},
  series = {Single Moleculus},
  journal   = {Single Moleculus},
  number    = {3(4)},
  pages     = {211 -- 216},
  subject      = {Cells},
  language  = {en}
}
@article{TeuschlAignerHohlriederetal.,
  author    = {Teuschl, Andreas and Aigner, Elmar and Hohlrieder, Martin and Cicha, Klaus and Stampfl, J{\"u}rgen and Redl, Heinz},
  title     = {Stimulation of ligament tissue formation on a silk scaffold with mechanical loading using a custom-made bioreactor system},
  series = {Journal of Tissue Engineering and Regenerative Medicine},
  volume    = {1},
  journal   = {Journal of Tissue Engineering and Regenerative Medicine},
  number    = {6},
  pages     = {51 -- 51},
  subject      = {Ligament},
  language  = {en}
}
@article{TeuschlFergusonSzomolanyietal.,
  author    = {Teuschl, Andreas and Ferguson, James and Szomolanyi, Pavol and Trattnig, Siegfried and Redl, Heinz and Nau, Thomas},
  title     = {Osteointegration of anterior cruciate ligament scaffolds fabricated of bombyx mori silk},
  series = {Journal of Tissue Engineering and Regenerative Medicine},
  volume    = {1},
  journal   = {Journal of Tissue Engineering and Regenerative Medicine},
  number    = {6},
  pages     = {181 -- 182},
  subject      = {Osteointegration},
  language  = {en}
}
@misc{RuenzlerBergmannSaraetal.,
  author    = {R{\"u}nzler, Dominik and Bergmann, J. and S{\´a}ra, Margit and K{\"o}hler, Gottfried},
  title     = {Protein-Ligand Interactions: Fluorescence Binding Assays performed with FCS and Fluorescence Anisotropy Measurements},
  subject      = {Protein},
  language  = {en}
}
@misc{MaierRuenzlerSchindelaretal.,
  author    = {Maier, Christina and R{\"u}nzler, Dominik and Schindelar, Julia and Grabner, Gottfried and Luger, Anton},
  title     = {High-Affinity Membrane Glucocorticoid Binding Sites on the Living Cell},
  subject      = {Cell Membrane},
  language  = {en}
}
@misc{RuenzlerSaraKoehler,
  author    = {R{\"u}nzler, Dominik and S{\´a}ra, Margit and K{\"o}hler, Gottfried},
  title     = {Biophysical Investigations on the S-layer Protein SbsB},
  subject      = {Protein},
  language  = {en}
}
@misc{Ruenzler,
  author    = {R{\"u}nzler, Dominik},
  title     = {Detection of Ultrafine Particles in Living Cells by using Fluorescence Correlation Spectroscopy},
  subject      = {Particle Detection},
  language  = {en}
}
@misc{RuenzlerMaierSchindelaretal.,
  author    = {R{\"u}nzler, Dominik and Maier, Christina and Schindelar, Julia and Grabner, Gottfried and K{\"o}hler, Gottfried and Luger, Anton},
  title     = {High-affinity membrane glucocorticoid binding sites on the pituitary cell line AtT-20 characterized by Fluorescence Correlation Spectroscopy},
  subject      = {Cell},
  language  = {en}
}
@misc{RuenzlerMaierWagneretal.,
  author    = {R{\"u}nzler, Dominik and Maier, Christina and Wagner, Ludwig and Grabner, Gottfried and K{\"o}hler, Gottfried and Luger, Anton},
  title     = {Evidence for specific glucocorticoid binding sites on the cell membrane of a living cell by fluorescence correlation microscopy},
  subject      = {Glucocorticoid},
  language  = {en}
}
@misc{RuenzlerMaierSchindelaretal.,
  author    = {R{\"u}nzler, Dominik and Maier, Christina and Schindelar, Julia and K{\"o}hler, Gottfried and Luger, Anton},
  title     = {Flourescence Correlation Spectroscopy (FCS): A New Tool for Studying Membrane Steroid Receptors},
  subject      = {Spectroscopy},
  language  = {en}
}
@misc{MaierRuenzlerWagneretal.,
  author    = {Maier, Christina and R{\"u}nzler, Dominik and Wagner, Ludwig and Grabner, Gottfried and Luger, Anton},
  title     = {Functional Characterization of the Putative Membrane Glucocorticoid Receptor using FCS},
  subject      = {Cell Membrane},
  language  = {en}
}
@misc{Ruenzler,
  author    = {R{\"u}nzler, Dominik},
  title     = {Investigation of Putative Membrane Glucocorticoid Binding Sites on Single Living Cells by FCS},
  subject      = {Cell Membrane},
  language  = {en}
}
@misc{EdetsbergerRuenzlerSchindelaretal.,
  author    = {Edetsberger, Michael and R{\"u}nzler, Dominik and Schindelar, Julia and Valic, Eva and K{\"o}hler, Gottfried},
  title     = {Specific glucocorticoid binding sites on the cell membrane of AtT-20 cell line},
  series = {11th Meeting of the European Neuroendocrine Association (ENEA)},
  journal   = {11th Meeting of the European Neuroendocrine Association (ENEA)},
  subject      = {Cell Membrane},
  language  = {en}
}
@article{RosnerSchipanyGundackeretal.,
  author    = {Rosner, Margit and Schipany, Katharina and Gundacker, Claudia and Shanmugasundaram, Parthasaraty and Li, Kongzhao and Fuchs, Christiane and Lubec, Gert and Hengstschl{\"a}ger, Martin},
  title     = {Renal differentiation of amniotic fluid stem cells: perspectives for clinical application and for studies on specific human genetic diseases},
  series = {Eur J Clin Invest},
  journal   = {Eur J Clin Invest},
  subject      = {Stem Cells},
  language  = {en}
}
@article{GundackerScheinastDamjanovicetal.,
  author    = {Gundacker, Claudia and Scheinast, Matthias and Damjanovic, Lukas and Fuchs, Christiane and Rosner, Margit and Hengstschl{\"a}ger, Markus},
  title     = {Proliferation potential of human amniotic fluid stem cells differently respondes to mercury and lead exposure},
  series = {Amino Acids},
  journal   = {Amino Acids},
  subject      = {Stem Cells},
  language  = {en}
}
@article{RosnerFuchsDolznigetal.,
  author    = {Rosner, Margit and Fuchs, Christiane and Dolznig, Helmut and Hengstschl{\"a}ger, Markus},
  title     = {Different cytoplasmic/nucelar distribution of S6 protein phosphorylated at S240/244 and S235/236},
  series = {Amino Acids},
  journal   = {Amino Acids},
  number    = {40},
  pages     = {595 -- 600},
  subject      = {Proteins},
  language  = {en}
}
@misc{MaierAltenbergerRuenzler,
  author    = {Maier, Christina and Altenberger, T. and R{\"u}nzler, Dominik},
  title     = {Investigation of Putative Membrane Glucocorticoid Binding Sites on Single Living Cells by FCS},
  subject      = {Cell Membrane},
  language  = {en}
}
@misc{EdetsbergerRuenzlerSchindelaretal.,
  author    = {Edetsberger, Michael and R{\"u}nzler, Dominik and Schindelar, Julia and Valic, Eva and K{\"o}hler, Gottfried},
  title     = {Detection of Ultrafine Particles in Living Cells},
  subject      = {Cell},
  language  = {en}
}
@article{KrautwaldBuescherJesenbergeretal.,
  author    = {Krautwald, Stefan and B{\"u}scher, Dirk and Jesenberger, Veronika and Buder, Sylke and Baccarini, Manuela},
  title     = {Involvement of the protein tyrosine phosphatase SHP-1 in Ras-mediated activation of the mitogen-activated protein kinase pathway.},
  series = {Mol Cell Biol.},
  volume    = {1996},
  journal   = {Mol Cell Biol.},
  number    = {16(11)},
  doi       = {doi: 10.1128/mcb.16.11.5955},
  pages     = {5955 -- 5963},
  abstract  = {Ubiquitously expressed SH2-containing tyrosine phosphatases interact physically with tyrosine kinase receptors or their substrates and relay positive mitogenic signals via the activation of the Ras-mitogen-activated protein kinase (MAPK) pathway. Conversely, the structurally related phosphatase SHP-1 is predominantly expressed in hemopoietic cells and becomes tyrosine phosphorylated upon colony-stimulating factor 1 treatment of macrophages without associating with the colony-stimulating factor 1 receptor tyrosine kinase. Mice lacking functional SHP-1 (me/me and me(v)/me(v)) develop systemic autoimmune disease with accumulation of macrophages, suggesting that SHP-1 may be a negative regulator of hemopoietic cell growth. By using macrophages expressing dominant negative Ras and the me(v)/me(v) mouse mutant, we show that SHP-1 is activated in the course of mitogenic signal transduction in a Ras-dependent manner and that its activity is necessary for the Ras-dependent activation of the MAPK pathway but not of the Raf-1 kinase. Consistent with a role for SHP-1 as an intermediate between Ras and the MEK-MAPK pathway, Ras-independent activation of the latter kinases by bacterial lipopolysaccharide occurred normally in me(v)/me(v) cells. Our results sharply accentuate the diversity of signal transduction in mammalian cells, in which the same signaling intermediates can be rearranged to form different pathways.},
  subject      = {Molecular Cell Biology},
  language  = {en}
}
@article{JesenbergerProcykYuanetal.,
  author    = {Jesenberger, Veronika and Procyk, Katarzyna and Yuan, Junying and Reipert, Siegfried and Baccarini, Manuela},
  title     = {Salmonella-induced caspase-2 activation in macrophages: a novel mechanism in pathogen-mediated apoptosis},
  series = {J Exp Med.},
  volume    = {2000},
  journal   = {J Exp Med.},
  number    = {192(7)},
  doi       = {10.1084/jem.192.7.1035},
  pages     = {1035 -- 1046},
  abstract  = {The enterobacterial pathogen Salmonella induces phagocyte apoptosis in vitro and in vivo. These bacteria use a specialized type III secretion system to export a virulence factor, SipB, which directly activates the host's apoptotic machinery by targeting caspase-1. Caspase-1 is not involved in most apoptotic processes but plays a major role in cytokine maturation. We show that caspase-1-deficient macrophages undergo apoptosis within 4-6 h of infection with invasive bacteria. This process requires SipB, implying that this protein can initiate the apoptotic machinery by regulating components distinct from caspase-1. Invasive Salmonella typhimurium targets caspase-2 simultaneously with, but independently of, caspase-1. Besides caspase-2, the caspase-1-independent pathway involves the activation of caspase-3, -6, and -8 and the release of cytochrome c from mitochondria, none of which occurs during caspase-1-dependent apoptosis. By using caspase-2 knockout macrophages and chemical inhibition, we establish a role for caspase-2 in both caspase-1-dependent and -independent apoptosis. Particularly, activation of caspase-1 during fast Salmonella-induced apoptosis partially relies on caspase-2. The ability of Salmonella to induce caspase-1-independent macrophage apoptosis may play a role in situations in which activation of this protease is either prevented or uncoupled from the induction of apoptosis.},
  subject      = {caspase-2 activation in macrophages},
  language  = {en}
}
@article{JesenbergerProcykRuethetal.,
  author    = {Jesenberger, Veronika and Procyk, Katarzyna and R{\"u}th, Jochen and Schreiber, Martin and Theussl, Hans Christian and Wagner, Erwin and Baccarini, Manuela},
  title     = {Protective Role of Raf-1 in Salmonella-Induced Macrophage Apoptosis},
  series = {J Exp Med.},
  volume    = {2001},
  journal   = {J Exp Med.},
  number    = {193(3)},
  doi       = {10.1084/jem.193.3.353},
  pages     = {353 -- 364},
  abstract  = {Invasive Salmonella induces macrophage apoptosis via the activation of caspase-1 by the bacterial protein SipB. Here we show that infection of macrophages with Salmonella causes the activation and degradation of Raf-1, an important intermediate in macrophage proliferation and activation. Raf-1 degradation is SipB- and caspase-1-dependent, and is prevented by proteasome inhibitors. To study the functional significance of Raf-1 in this process, the c-raf-1 gene was inactivated by Cre-loxP-mediated recombination in vivo. Macrophages lacking c-raf-1 are hypersensitive towards pathogen-induced apoptosis. Surprisingly, activation of the antiapoptotic mitogen-activated protein kinase kinase (MEK)/extracellular signal-regulated kinase (ERK) and nuclear factor κB pathways is normal in Raf-1-deficient macrophages, and mitochondrial fragility is not increased. Instead, pathogen-mediated activation of caspase-1 is enhanced selectively, implying that Raf-1 antagonizes stimulus-induced caspase-1 activation and apoptosis.},
  subject      = {Salmonella-Induced Macrophage Apoptosis},
  language  = {en}
}
@article{JesenbergerJentsch,
  author    = {Jesenberger, Veronika and Jentsch, Stefan},
  title     = {Deadly encounter: ubiquitin meets apoptosis},
  series = {Nat Rev Mol Cell Biol.},
  volume    = {2002},
  journal   = {Nat Rev Mol Cell Biol.},
  number    = {3(2)},
  doi       = {https://doi.org/10.1038/nrm731},
  pages     = {112 -- 121},
  abstract  = {The ubiquitin/proteasome pathway is the main non-lysosomal route for intracellular protein degradation in eukaryotes. It is instrumental to various cellular processes, such as cell-cycle progression, transcription and antigen processing. Recent findings also substantiate a pivotal role of the ubiquitin/proteasome pathway in the regulation of apoptosis. Regulatory molecules that are involved in programmed cell death have been identified as substrates of the proteasome. Moreover, key regulators of apoptosis themselves seem to have an active part in the proteolytic inactivation of death executors.},
  subject      = {programmed cell death},
  language  = {en}
}
@misc{LeitnerSauermann,
  author    = {Leitner, Rita and Sauermann, Stefan},
  title     = {Erreichen „harmonisierter Empfehlungen": von Studierenden generierte Fragen als Ausgangspunkt f{\"u}r evidenzbasierte Argumentation von Stakeholder-Sichtweisen - ein problem-based learning Ansatz},
  subject      = {Problem Based Learning},
  language  = {de}
}
@article{HuberGriesIlkRuenzleretal.,
  author    = {Huber-Gries, Carina and Ilk, Nicola and R{\"u}nzler, Dominik and Egelseer, Eva-Maria and Weigert, Stefan and Sleytr, Uwe},
  title     = {The three S-layer-like homology motifs of the S-layer protein SbpA of Bacillus sphaericus CCM 2177 are not sufficient for binding to the pyruvylated secondary cell wall polymer},
  series = {Molecular Microbiology},
  volume    = {55},
  journal   = {Molecular Microbiology},
  number    = {1},
  pages     = {197 -- 205},
  subject      = {Proteins},
  language  = {en}
}
@misc{ThieleKafkaLitzenbergeretal.,
  author    = {Thiele, Gerrit and Kafka, Patricia and Litzenberger, Stefan and Sabo, Anton},
  title     = {Ontrack measurements in motocross: The correlation of neck muscle activity and contact incidents of helmet and neck brace},
  subject      = {Motocross},
  language  = {en}
}
@article{TomaschMaleinerHromadaetal.,
  author    = {Tomasch, Janine and Maleiner, Babette and Hromada, Carina and Szwarc-Hofbauer, Dorota and Teuschl-Woller, Andreas},
  title     = {Cyclic Tensile Stress Induces Skeletal Muscle Hypertrophy and Myonuclear Accretion in a 3D Model},
  series = {Tissue Eng. Part A.},
  volume    = {2023},
  journal   = {Tissue Eng. Part A.},
  number    = {Mar},
  pages     = {257 -- 268},
  abstract  = {Skeletal muscle is highly adaptive to mechanical stress due to its resident stem cells and the pronounced level of myotube plasticity. Herein, we study the adaptation to mechanical stress and its underlying molecular mechanisms in a tissue-engineered skeletal muscle model. We subjected differentiated 3D skeletal muscle-like constructs to cyclic tensile stress using a custom-made bioreactor system, which resulted in immediate activation of stress-related signal transducers (Erk1/2, p38). Cell cycle re-entry, increased proliferation, and onset of myogenesis indicated subsequent myoblast activation. Furthermore, elevated focal adhesion kinase and β-catenin activity in mechanically stressed constructs suggested increased cell adhesion and migration. After 3 days of mechanical stress, gene expression of the fusogenic markers MyoMaker and MyoMixer, myotube diameter, myonuclear accretion, as well as S6 activation, were significantly increased. Our results highlight that we established a promising tool to study sustained adaptation to mechanical stress in healthy, hypertrophic, or regenerating skeletal muscle.},
  subject      = {fibrin},
  language  = {en}
}
@misc{EgelseerVoellenklePleschbergeretal.,
  author    = {Egelseer, Eva-Maria and V{\"o}llenkle, Christine and Pleschberger, Magdalena and Moll, Dieter and Ilk, Nicola and Huber-Gries, Carina and Breitwieser, A. and Sleytr, Uwe and S{\´a}ra, Margit},
  title     = {The relevance of S-layer fusion proteins in nanotechnology},
  subject      = {Protein},
  language  = {en}
}
@misc{StoiberPurtscherGeppetal.,
  author    = {Stoiber, Stefan and Purtscher, Michaela and Gepp, Barbara and Huber-Gries, Carina},
  title     = {Towards a platform for spatially defined cell characterization of a miniturized heart tissue model},
  subject      = {Microfluidic},
  language  = {en}
}
@misc{PurtscherErgirSzwarcetal.,
  author    = {Purtscher, Michaela and Ergir, Ece and Szwarc, Dorota and Monforte Vila, Xavier and R{\"u}nzler, Dominik and Huber-Gries, Carina},
  title     = {A microfluidic-based easy-to-use cardiac tissue model for drug screening applications},
  subject      = {Tissue Generation},
  language  = {en}
}
@misc{PurtscherErgirMonforteVilaetal.,
  author    = {Purtscher, Michaela and Ergir, Ece and Monforte Vila, Xavier and Huber-Gries, Carina},
  title     = {Establishment of an in vitro heart tissue model for pre-clinical screening of therapeutic agents using microfluidic technology},
  subject      = {Tissue Generation},
  language  = {en}
}
@misc{PurtscherErgirSzwarcetal.,
  author    = {Purtscher, Michaela and Ergir, Ece and Szwarc, Dorota and Huber-Gries, Carina},
  title     = {Microfluidic based heart-tissue model for directed development of cardiac specific cell types},
  subject      = {Heart Tissue},
  language  = {en}
}
@misc{HromadaTomaschWeihsetal.,
  author    = {Hromada, Carina and Tomasch, Janine and Weihs, Anna and R{\"u}nzler, Dominik and Teuschl, Andreas},
  title     = {Engineering of 3D Tissue Constructs Using our Novel MagneTissue Bioreactor as Alternatives to Animal Models},
  subject      = {Bioreactor},
  language  = {en}
}
@misc{LitzenbergerMallyBraunsteinetal.,
  author    = {Litzenberger, Stefan and Mally, Franziska and Braunstein, Bj{\"o}rn and Willwacher, Steffen and Sabo, Anton and Br{\"u}ggemann, Gert-Peter},
  title     = {Influence of weighted cuffs on ground reaction forces in running of an elite unilateral upper extremity amputee athlete.},
  subject      = {Amputation},
  language  = {en}
}
@misc{LitzenbergerMallyWillwacheretal.,
  author    = {Litzenberger, Stefan and Mally, Franziska and Willwacher, Steffen and Braunstein, Bj{\"o}rn and Sabo, Anton and Br{\"u}ggemann, Gert-Peter},
  title     = {Einfluss einer unilateralen Dysmelie des Unterarms auf die Bodenreaktionskr{\"a}fte eines 800m L{\"a}ufers},
  subject      = {Running},
  language  = {de}
}
@misc{FelsnerLitzenbergerMallyetal.,
  author    = {Felsner, Eduard-Max and Litzenberger, Stefan and Mally, Franziska and Sabo, Anton},
  title     = {Musculoskeletal modelling of elite handcycling motion: Evaluation of muscular on- and offset},
  subject      = {Handcycling},
  language  = {en}
}
@misc{LitzenbergerSaboFuss,
  author    = {Litzenberger, Stefan and Sabo, Anton and Fuss, Franz Konstantin},
  title     = {Effect of different mounting angles of prosthetic feet dedicated to sprinting on reaction forces},
  subject      = {Prothesis},
  language  = {en}
}
@article{LeebLeitnerPichleretal.,
  author    = {Leeb, Christine and Leitner, Rita and Pichler, Verena and Huber-Gries, Carina and R{\"u}nzler, Dominik and Jesenberger, Veronika},
  title     = {Einf{\"u}hrung und Optimierung eines praxisorientierten PBL-Moduls im Life-Science-Bereich},
  series = {Zeitschrift f{\"u}r Hochschulentwicklung (ZFHE)},
  volume    = {11},
  journal   = {Zeitschrift f{\"u}r Hochschulentwicklung (ZFHE)},
  number    = {3},
  pages     = {107 -- 121},
  subject      = {Life Science},
  language  = {de}
}
@article{RiederWeihsWeidingeretal.,
  author    = {Rieder, Bernhard and Weihs, Anna and Weidinger, Adelheid and Sczwarc, Dorota and N{\"u}rnberger, Sylvia and Redl, Heinz and R{\"u}nzler, Dominik and Huber-Gries, Carina and Teuschl, Andreas},
  title     = {Hydrostatic pressure-generated reactive oxygen species induce osteoarthritic conditions in cartilage pellet cultures},
  series = {Scientific Reports},
  journal   = {Scientific Reports},
  subject      = {Bioreactor},
  language  = {en}
}
@article{TeuschlZipperleHuberGriesetal.,
  author    = {Teuschl, Andreas and Zipperle, Johannes and Huber-Gries, Carina and Kaplan, David},
  title     = {Silk fibroin based carrier system for delivery of fibrinogen and thrombin as coagulant supplements},
  series = {Journal of Biomedical Materials Research},
  volume    = {105},
  journal   = {Journal of Biomedical Materials Research},
  number    = {3},
  subject      = {Fibrin},
  language  = {en}
}
@inproceedings{HuberGriesMollLiuetal.,
  author    = {Huber-Gries, Carina and Moll, Dieter and Liu, Jing and R{\"u}nzler, Dominik and S{\´a}ra, Margit and Sleytr, Uwe},
  title     = {S-layer-streptavidin fusion proteins: Novel Tools in Nanobiotechnology},
  series = {6th Annual Linz Winter Workshop - Book of Abstracts},
  booktitle = {6th Annual Linz Winter Workshop - Book of Abstracts},
  pages     = {11 -- 11},
  subject      = {Proteins},
  language  = {en}
}
@article{HuberGries,
  author    = {Huber-Gries, Carina},
  title     = {Heterotetramers Formed by an S-Layer-Streptavidin Fusion Protein and Core-Streptavidin as a Nanoarrayed Template for Biochip Development},
  series = {Small},
  volume    = {2},
  journal   = {Small},
  number    = {1},
  pages     = {142 -- 150},
  subject      = {Biochips},
  language  = {en}
}
@article{SleytrHuberGriesNicolaetal.,
  author    = {Sleytr, Uwe B. and Huber-Gries, Carina and Nicola, Ilk and Puhm, Dietmar and Schuster, Bernhard and Egelseer, Eva-Maria},
  title     = {S-layers as a tool kit for nanobiotechnological applications},
  series = {FEMS Microbiology Letters},
  journal   = {FEMS Microbiology Letters},
  number    = {267(2)},
  subject      = {Nanobiotechnology},
  language  = {en}
}
@misc{KamravamaneshPflueglHerwigetal.,
  author    = {Kamravamanesh, Donya and Pfl{\"u}gl, Stefan and Herwig, Christoph and Lackner, Maximilian},
  title     = {Optimization of process parameters to enhance PHA accumulation in Synechosystis sp. PCC 6714 using multivariate design of experiments},
  subject      = {Bacteria},
  language  = {en}
}
@article{EbnerKienbergerHuberGriesetal.,
  author    = {Ebner, Andreas and Kienberger, Ferry and Huber-Gries, Carina and Kamruzzahan, A.S.M. and Pastushenko, Vassili and Tang, Jilin and Kada, Gerald and Gruber, Hermann and Sleytr, Uwe and S{\´a}ra, Margit and Hinterdorfer, Peter},
  title     = {Atomic-force-microscopy imaging and molecular-recognition-force microscopy of recrystallized heterotetramers comprising an S-layer-streptavidin fusion protein},
  series = {ChemBioChem},
  volume    = {7},
  journal   = {ChemBioChem},
  number    = {4},
  pages     = {588 -- 591},
  subject      = {Microscopy},
  language  = {en}
}
@inproceedings{HuberGriesLiuEgelseeretal.,
  author    = {Huber-Gries, Carina and Liu, Jing and Egelseer, Eva-Maria and R{\"u}nzler, Dominik and S{\´a}ra, Margit and Sleytr, Uwe},
  title     = {Application Potential of Bacterial Self-Assembly Systems for Nanobiotechnology},
  series = {Nanobionics III (from Molecules to Applications) Book of Abstracts},
  booktitle = {Nanobionics III (from Molecules to Applications) Book of Abstracts},
  subject      = {Nanotechnology},
  language  = {en}
}
@article{SaraviaNolteHuberGriesetal.,
  author    = {Saravia, Veronica and Nolte, M. and Huber-Gries, Carina and Puhm, Dietmar and Fery, Andreas and Sleytr, Uwe B. and Toca-Herrera, Jose Luis},
  title     = {Bacterial protein patterning by micro-contact printing of PLL-g-PEG},
  series = {Journal of Biotechnology},
  journal   = {Journal of Biotechnology},
  number    = {130(3)},
  pages     = {247 -- 252},
  subject      = {Proteins},
  language  = {en}
}
@article{RuenzlerHuberGriesMolletal.,
  author    = {R{\"u}nzler, Dominik and Huber-Gries, Carina and Moll, Dieter and S{\´a}ra, Margit},
  title     = {Biophysical characterization of the entire bacterial surface layer protein SbsB and its two distinct functional domains},
  series = {Journal of Biological Chemistry},
  journal   = {Journal of Biological Chemistry},
  number    = {279(7) / 13.},
  pages     = {5207 -- 5215},
  subject      = {Life Sciences},
  language  = {en}
}
@inproceedings{HuberGriesMollSchlegeletal.,
  author    = {Huber-Gries, Carina and Moll, Dieter and Schlegel, Birgit and R{\"u}nzler, Dominik and Mader, Christoph and S{\´a}ra, Margit and Sleytr, Uwe},
  title     = {Recombinant S-layer-streptavidin fursion proteins: Functional protein lattices as nanoarrays for biochip development},
  series = {Proceedings der 28. Jahrestagung der {\"O}sterreichischen Gesellschaft f{\"u}r Hygiene, Mikrobiologie und Pr{\"a}ventivmedizin, Merano (Italy)},
  booktitle = {Proceedings der 28. Jahrestagung der {\"O}sterreichischen Gesellschaft f{\"u}r Hygiene, Mikrobiologie und Pr{\"a}ventivmedizin, Merano (Italy)},
  subject      = {Proteins},
  language  = {en}
}
@inproceedings{LeebLeitnerPichleretal.,
  author    = {Leeb, Christine and Leitner, Rita and Pichler, Verena and Huber-Gries, Carina and R{\"u}nzler, Dominik and Jesenberger, Veronika},
  title     = {Einf{\"u}hrung und Optimierung eines praxisorientierten PBL-Moduls im Life-Science-Bereich},
  series = {Proceedings des Kongresses Problem-Based Learning 2016 PBL - Kompetenzen f{\"o}rdern, Zukunft gestalten},
  booktitle = {Proceedings des Kongresses Problem-Based Learning 2016 PBL - Kompetenzen f{\"o}rdern, Zukunft gestalten},
  subject      = {Life Science},
  language  = {de}
}
@inproceedings{MollHuberGriesSchlegeletal.,
  author    = {Moll, Dieter and Huber-Gries, Carina and Schlegel, Birgit and Pum, Dietmar and Sleytr, Uwe and S{\´a}ra, Margit},
  title     = {S-layer-streptavidin fursion protein as template for nanopatterned molecular arrays},
  series = {Proceedings of the National Academy of Sciences USA},
  booktitle = {Proceedings of the National Academy of Sciences USA},
  pages     = {14646 -- 14651},
  subject      = {Proteins},
  language  = {en}
}
@misc{RuenzlerMollHuberGriesetal.,
  author    = {R{\"u}nzler, Dominik and Moll, Dieter and Huber-Gries, Carina and Sleytr, Uwe and S{\´a}ra, Margit and K{\"o}hler, Gottfried},
  title     = {Characterization of the Functional Domains of Bacterial Surface Layer Proteins},
  series = {5th European Symposium of the Protein Society},
  journal   = {5th European Symposium of the Protein Society},
  subject      = {Proteins},
  language  = {en}
}
@misc{LeebLeitnerPichleretal.,
  author    = {Leeb, Christine and Leitner, Rita and Pichler, Verena and Huber-Gries, Carina and R{\"u}nzler, Dominik and Jesenberger, Veronika},
  title     = {Einf{\"u}hrung und Optimierung eines praxisorientierten PBL-Moduls im Life-Science-Bereich},
  subject      = {Life Science},
  language  = {de}
}
@article{MaderHuberGriesMolletal.,
  author    = {Mader, Christoph and Huber-Gries, Carina and Moll, Dieter and Sleytr, Uwe and S{\´a}ra, Margit},
  title     = {Interaction of the crystalline bacterial cell surface layer protein SbsB and the secondary cell wall polymer of Geobacillus stearothermophilus PV72 assessed by real-time surface plasmon resonance biosensor technology},
  series = {Journal of Bacteriology},
  volume    = {186},
  journal   = {Journal of Bacteriology},
  number    = {6},
  pages     = {1758 -- 1768},
  subject      = {Bacteria},
  language  = {en}
}
@misc{RuenzlerHuberGriesSaraetal.,
  author    = {R{\"u}nzler, Dominik and Huber-Gries, Carina and S{\´a}ra, Margit and K{\"o}hler, Gottfried},
  title     = {Fluorescence-based Equilibrium and Kinetic Studies on Carbohydrate-Protein Binding: Interaction of Bacterial Surface Layer Proteins with their Specific Secondary Cell Wall Polymers},
  subject      = {Fluorescence},
  language  = {en}
}
@inproceedings{HuberGriesMollLiuetal.,
  author    = {Huber-Gries, Carina and Moll, Dieter and Liu, Jing and R{\"u}nzler, Dominik and S{\´a}ra, Margit and Sleytr, Uwe B.},
  title     = {Chimaeric S-Layers as Nanopatterned Biomaterials for Applications in Nanobiotechnology},
  series = {Poster presentation at the 7th Annual Linz Winter Workshop / Book of Abstracts / Linz},
  booktitle = {Poster presentation at the 7th Annual Linz Winter Workshop / Book of Abstracts / Linz},
  pages     = {1 -- 10},
  subject      = {Biomaterial},
  language  = {en}
}
@article{TangEbnerIlketal.,
  author    = {Tang, Jilin and Ebner, Andreas and Ilk, Nicola and Lichtblau, Helga and Huber-Gries, Carina and Zhu, Rong and Pum, Dietmar and Leitner, Michael and Pastushenko, Vassili Filippovich and Gruber, Hermann and Sleytr, Uwe B. and Hinterdorfer, Peter},
  title     = {High-affinity tags fused to s-layer proteins probed by atomic force microscopy},
  series = {Langmuir},
  journal   = {Langmuir},
  number    = {24(4)},
  pages     = {1324 -- 1329},
  subject      = {Proteins},
  language  = {en}
}
@misc{DavidForjanSchereretal.,
  author    = {David, Veronika and Forjan, Mathias and Scherer, Matthias and Reichel, Martin},
  title     = {Entwicklung und Vorstudien zur Implementierung mobiler Rehabilitationssysteme im h{\"a}uslichen Umfeld},
  subject      = {Rehabilitation},
  language  = {de}
}
@article{TvorogovAnisimovZhengetal.,
  author    = {Tvorogov, Denis and Anisimov, Andrey and Zheng, Wei and Lepp{\´a}nen, Veli-Matti and Tammela, Tuomas and Laurinavicius, Simonas and Holnthoner, Wolfgang and Helotera, Hanna and Holopainen, Tanja and Jeltsch, Michael and Kalkkinen, Nisse and Lankinen, Hikka and Ojala, P{\"a}ivi M. and Alitalo, Kari},
  title     = {Effective Suppression of Vascular Network Formation by Combination of Antibodies Blocking VEGFR Ligand Binding and Receptor Dimerization},
  series = {Cancer Cell},
  journal   = {Cancer Cell},
  number    = {18(6)},
  pages     = {630 -- 640},
  subject      = {Vascular Network},
  language  = {en}
}
@misc{MuehlederFuchsBassilioetal.,
  author    = {M{\"u}hleder, Severin and Fuchs, Christiane and Bassilio, Jose and Sczwarc, Dorota and Pill, Karoline and Slezak, Paul and Labuda, Krystina and Siehs, Christian and Pr{\"o}ll, Johannes and Priglinger, Eleni and Redl, Heinz and Holnthoner, Wolfgang},
  title     = {The purinergic receptor P2Y2 modulates endothelial sprouting and angiogenesis},
  subject      = {Angiogenesis},
  language  = {en}
}
@article{SimsaPadmaHeheretal.,
  author    = {Simsa, Robin and Padma, Arvind and Heher, Philipp and Hellstr{\"o}m, Mats and Teuschl, Andreas and Jenndahl, Lachmi and Bergh, Niklas and Fogelstrand, Per},
  title     = {Systematic in vitro comparison of decellularization protocols for blood vessels.},
  series = {PLoS One},
  journal   = {PLoS One},
  subject      = {Tissue Engineering},
  language  = {en}
}
@article{NuernbergerSchneiderKeibletal.,
  author    = {N{\"u}rnberger, S. and Schneider, C. and Keibl, C. and Sch{\"a}dl, Barbara and Heimel, P. and Monforte, X. and Teuschl, A. H. and Nalbach, M. and Thurner, P. J. and Grillari, J. and Redl, Heinz and Wolbank, S.},
  title     = {Repopulation of decellularised articular cartilage by laser-based matrix engraving},
  series = {EBioMedicine.},
  volume    = {64},
  journal   = {EBioMedicine.},
  number    = {103196.},
  abstract  = {Background: In spite of advances in the treatment of cartilage defects using cell and scaffold-based therapeutic strategies, the long-term outcome is still not satisfying since clinical scores decline years after treatment. Scaffold materials currently used in clinical settings have shown limitations in providing suitable biomechanical properties and an authentic and protective environment for regenerative cells. To tackle this problem, we developed a scaffold material based on decellularised human articular cartilage. Methods: Human articular cartilage matrix was engraved using a CO2 laser and treated for decellularisation and glycosaminoglycan removal. Characterisation of the resulting scaffold was performed via mechanical testing, DNA and GAG quantification and in vitro cultivation with adipose-derived stromal cells (ASC). Cell vitality, adhesion and chondrogenic differentiation were assessed. An ectopic, unloaded mouse model was used for the assessment of the in vivo performance of the scaffold in combination with ASC and human as well as bovine chondrocytes. The novel scaffold was compared to a commercial collagen type I/III scaffold. Findings: Crossed line engravings of the matrix allowed for a most regular and ubiquitous distribution of cells and chemical as well as enzymatic matrix treatment was performed to increase cell adhesion. The biomechanical characteristics of this novel scaffold that we term CartiScaff were found to be superior to those of commercially available materials. Neo-tissue was integrated excellently into the scaffold matrix and new collagen fibres were guided by the laser incisions towards a vertical alignment, a typical feature of native cartilage important for nutrition and biomechanics. In an ectopic, unloaded in vivo model, chondrocytes and mesenchymal stromal cells differentiated within the incisions despite the lack of growth factors and load, indicating a strong chondrogenic microenvironment within the scaffold incisions. Cells, most noticeably bone marrow-derived cells, were able to repopulate the empty chondrocyte lacunae inside the scaffold matrix. Interpretation: Due to the better load-bearing, its chondrogenic effect and the ability to guide matrix-deposition, CartiScaff is a promising biomaterial to accelerate rehabilitation and to improve long term clinical success of cartilage defect treatment. Funding: Austrian Research Promotion Agency FFG ("CartiScaff" \#842455), Lorenz B{\"o}hler Fonds (16/13), City of Vienna Competence Team Project Signaltissue (MA23, \#18-08). Keywords: Cartilage regeneration; Decellularisation; Ectopic animal model; Laser engraving; Mechanical testing; Repopulation.},
  subject      = {Tissue Engineering},
  language  = {en}
}
@article{SchneiderEnayatiGrasletal.,
  author    = {Schneider, Karl Heinrich and Enayati, Marjan and Grasl, Christian and Walter, Ingrid and Budinsky, Lubos and Zebic, Gabriel and Kaun, Christoph and Wagner, Anja and Kratochwill, Klaus and Redl, Heinz and Teuschl, Andreas and Podesser, Bruno K. and Bergmeister, Helga},
  title     = {Acellular vascular matrix grafts from human placenta chorion: Impact of ECM preservation on graft characteristics, protein composition and in vivo performance.},
  series = {Biomaterials},
  journal   = {Biomaterials},
  pages     = {14 -- 26},
  abstract  = {Small diameter vascular grafts from human placenta, decellularized with either Triton X-100 (Triton) or SDS and crosslinked with heparin were constructed and characterized. Graft biochemical properties, residual DNA, and protein composition were evaluated to compare the effect of the two detergents on graft matrix composition and structural alterations. Biocompatibility was tested in vitro by culturing the grafts with primary human macrophages and in vivo by subcutaneous implantation of graft conduits (n = 7 per group) into the flanks of nude rats. Subsequently, graft performance was evaluated using an aortic implantation model in Sprague Dawley rats (one month, n = 14). In situ graft imaging was performed using MRI angiography. Retrieved specimens were analyzed by electromyography, scanning electron microscopy, histology and immunohistochemistry to evaluate cell migration and the degree of functional tissue remodeling. Both decellularization methods resulted in grafts of excellent biocompatibility in vitro and in vivo, with low immunogenic potential. Proteomic data revealed removal of cytoplasmic proteins with relative enrichment of ECM proteins in decelluarized specimens of both groups. Noteworthy, LC-Mass Spectrometry analysis revealed that 16 proteins were exclusively preserved in Triton decellularized specimens in comparison to SDS-treated specimens. Aortic grafts showed high patency rates, no signs of thrombus formation, aneurysms or rupture. Conduits of both groups revealed tissue-specific cell migration indicative of functional remodeling. This study strongly suggests that decellularized allogenic grafts from the human placenta have the potential to be used as vascular replacement materials. Both detergents produced grafts with low residual immunogenicity and appropriate mechanical properties. Observed differences in graft characteristics due to preservation method had no impact on successful in vivo performance in the rodent model.},
  subject      = {Biomaterial},
  language  = {en}
}
@article{TeuschlHolnthonerMonforte,
  author    = {Teuschl, Andreas and Holnthoner, Wolfgang and Monforte, Xavier},
  title     = {Repopulation of an auricular cartilage scaffold, AuriScaff, perforated with an enzyme combination},
  series = {Acta Biomater.},
  volume    = {2019},
  journal   = {Acta Biomater.},
  number    = {Mar/86},
  pages     = {207 -- 222},
  abstract  = {Biomaterials currently in use for articular cartilage regeneration do not mimic the composition or architecture of hyaline cartilage, leading to the formation of repair tissue with inferior characteristics. In this study we demonstrate the use of "AuriScaff", an enzymatically perforated bovine auricular cartilage scaffold, as a novel biomaterial for repopulation with regenerative cells and for the formation of high-quality hyaline cartilage. AuriScaff features a traversing channel network, generated by selective depletion of elastic fibers, enabling uniform repopulation with therapeutic cells. The complex collagen type II matrix is left intact, as observed by immunohistochemistry, SEM and TEM. The compressive modulus is diminished, but three times higher than in the clinically used collagen type I/III scaffold that served as control. Seeding tests with human articular chondrocytes (hAC) alone and in co-culture with human adipose-derived stromal/stem cells (ASC) confirmed that the network enabled cell migration throughout the scaffold. It also guides collagen alignment along the channels and, due to the generally traverse channel alignment, newly deposited cartilage matrix corresponds with the orientation of collagen within articular cartilage. In an osteochondral plug model, AuriScaff filled the complete defect with compact collagen type II matrix and enabled chondrogenic differentiation inside the channels. Using adult articular chondrocytes from bovine origin (bAC), filling of even deep defects with high-quality hyaline-like cartilage was achieved after 6 weeks in vivo. With its composition and spatial organization, AuriScaff provides an optimal chondrogenic environment for therapeutic cells to treat cartilage defects and is expected to improve long-term outcome by channel-guided repopulation followed by matrix deposition and alignment. STATEMENT OF SIGNIFICANCE: After two decades of tissue engineering for cartilage regeneration, there is still no optimal strategy available to overcome problems such as inconsistent clinical outcome, early and late graft failures. Especially large defects are dependent on biomaterials and their scaffolding, guiding and protective function. Considering the currently used biomaterials, structure and mechanical properties appear to be insufficient to fulfill this task. The novel scaffold developed within this study is the first approach enabling the use of dense cartilage matrix, repopulate it via channels and provide the cells with a compact collagen type II environment. Due to its density, it also provides better mechanical properties than materials currently used in clinics. We therefore think, that the auricular cartilage scaffold (AuriScaff) has a high potential to improve future cartilage regeneration approaches.},
  subject      = {Auricular cartilage},
  language  = {en}
}
@misc{RankTraxlerBayeretal.,
  author    = {Rank, Elisabet and Traxler, Lukas and Bayer, Natascha and Reutterer, Bernd and Lux, Kirsten and Drauschke, Andreas},
  title     = {Reproducibility analysis of measurements with a mechanical semiautomatic eye model for evaluation of intraocular lenses},
  subject      = {Mechanical Eye},
  language  = {en}
}
@misc{ObergruberMehnen,
  author    = {Obergruber, Julian and Mehnen, Lars},
  title     = {Development of a paraglide control system for automatic pitch stabilization to increase the passive safety},
  subject      = {Paragliding},
  language  = {en}
}
@article{PriglingerSchuhSteffenhagenetal.,
  author    = {Priglinger, Eleni and Schuh, Christina and Steffenhagen, Carolin and Wurzer, Christoph and Maier, Julia and N{\"u}rnberger, Sylvia and Holnthoner, Wolfgang and Fuchs, Christiane and Suessner, Susanne and R{\"u}nzler, Dominik and Redl, Heinz and Wolbank, Susanne},
  title     = {Improvement of adipose tissue-derived cells by low-energy extracorporeal shock wave therapy.},
  series = {Cytotherapy},
  journal   = {Cytotherapy},
  pages     = {1079 -- 1095},
  abstract  = {BACKGROUND: Cell-based therapies with autologous adipose tissue-derived cells have shown great potential in several clinical studies in the last decades. The majority of these studies have been using the stromal vascular fraction (SVF), a heterogeneous mixture of fibroblasts, lymphocytes, monocytes/macrophages, endothelial cells, endothelial progenitor cells, pericytes and adipose-derived stromal/stem cells (ASC) among others. Although possible clinical applications of autologous adipose tissue-derived cells are manifold, they are limited by insufficient uniformity in cell identity and regenerative potency. METHODS: In our experimental set-up, low-energy extracorporeal shock wave therapy (ESWT) was performed on freshly obtained human adipose tissue and isolated adipose tissue SVF cells aiming to equalize and enhance stem cell properties and functionality. RESULTS: After ESWT on adipose tissue we could achieve higher cellular adenosine triphosphate (ATP) levels compared with ESWT on the isolated SVF as well as the control. ESWT on adipose tissue resulted in a significantly higher expression of single mesenchymal and vascular marker compared with untreated control. Analysis of SVF protein secretome revealed a significant enhancement in insulin-like growth factor (IGF)-1 and placental growth factor (PLGF) after ESWT on adipose tissue. DISCUSSION: Summarizing we could show that ESWT on adipose tissue enhanced the cellular ATP content and modified the expression of single mesenchymal and vascular marker, and thus potentially provides a more regenerative cell population. Because the effectiveness of autologous cell therapy is dependent on the therapeutic potency of the patient's cells, this technology might raise the number of patients eligible for autologous cell transplantation.},
  subject      = {Shockwave Therapy},
  language  = {en}
}
@article{BachmannSpitzRothbaueretal.,
  author    = {Bachmann, Barbara and Spitz, Sarah and Rothbauer, Mario and Jordan, Christian and Purtscher, Michaela and Zirath, Helene and Schuller, Patrick and Eilenberger, Christoph and Ali, Syed Faheem and M{\"u}hleder, Severin and Priglinger, Eleni and Harasek, Michael and Redl, Heinz and Holnthoner, Wolfgang and Ertl, Peter},
  title     = {Engineering of three-dimensional pre-vascular networks within fibrin hydrogel constructs by microfluidic control over reciprocal cell signaling},
  series = {Biomicrofluidics},
  journal   = {Biomicrofluidics},
  subject      = {Microfluidic},
  language  = {en}
}
@article{CieślikBoczulaKuepcueRuenzleretal.,
  author    = {Cieślik-Boczula, Katarzyna and K{\"u}pc{\"u}, Seta and R{\"u}nzler, Dominik and Koll, Aleksander and K{\"o}hler, Gottfried},
  title     = {Effects of the phenolic lipid 3-pentadecylphenol on phospholipid bilayer organization},
  series = {Journal of Molecular Structure},
  journal   = {Journal of Molecular Structure},
  number    = {919(1-3)},
  pages     = {373 -- 380},
  subject      = {Phospholipid Bilayer},
  language  = {en}
}
@misc{SchneiderAignerMonforteVilaetal.,
  author    = {Schneider, Karl Heinrich and Aigner, Petra and Monforte Vila, Xavier and Holnthoner, Wolfgang and Teuschl, Andreas and Bergmeister, Helga and Redl, Heinz},
  title     = {Naturally derived acellular small diameter vascular grafts from human placenta for reconstructive surgery},
  subject      = {Placenta},
  language  = {en}
}
@article{SchneiderRohringerKapelleretal.,
  author    = {Schneider, Karl and Rohringer, Sabrina and Kapeller, Barbara and Grasl, Christian and Kiss, Herbert and Heber, Stefan and Walter, Ingrid and Teuschl, Andreas and Podesser, Bruno K. and Bergmeister, Helga},
  title     = {Riboflavin-mediated photooxidation to improve the characteristics of decellularized human arterial small diameter vascular grafts},
  series = {Acta Biomater.},
  volume    = {2020},
  journal   = {Acta Biomater.},
  number    = {116},
  pages     = {246 -- 258},
  abstract  = {Vascular grafts with a diameter of less than 6 mm are made from a variety of materials and techniques to provide alternatives to autologous vascular grafts. Decellularized materials have been proposed as a possible approach to create extracellular matrix (ECM) vascular prostheses as they are naturally derived and inherently support various cell functions. However, these desirable graft characteristics may be limited by alterations of the ECM during the decellularization process leading to decreased biomechanical properties and hemocompatibility. In this study, arteries from the human placenta chorion were decellularized using two distinct detergents (Triton X-100 or SDS), which differently affect ECM ultrastructure. To overcome biomechanical strength loss and collagen fiber exposure after decellularization, riboflavin-mediated UV (RUV) crosslinking was used to uniformly crosslink the collagenous ECM of the grafts. Graft characteristics and biocompatibility with and without RUV crosslinking were studied in vitro and in vivo. RUV-crosslinked ECM grafts showed significantly improved mechanical strength and smoothening of the luminal graft surfaces. Cell seeding using human endothelial cells revealed no cytotoxic effects of the RUV treatment. Short-term aortic implants in rats showed cell migration and differentiation of host cells. Functional graft remodeling was evident in all grafts. Thus, RUV crosslinking is a preferable tool to improve graft characteristics of decellularized matrix conduits.},
  subject      = {Tissue Engineering},
  language  = {en}
}
@article{SchneiderPultarOesterreicheretal.,
  author    = {Schneider, Jaana and Pultar, Marianne and Oesterreicher, Johannes and Bobbili, Madhusudhan Reddy and M{\"u}hleder, Severin and Priglinger, Eleni and Redl, Heinz and Spittler, Andreas and Grillari, Johannes and Holnthoner, Wolfgang},
  title     = {Cre mRNA Is Not Transferred by EVs from Endothelial and Adipose-Derived Stromal/Stem Cells during Vascular Network Formation},
  series = {Int J Mol Sci.},
  volume    = {2021},
  journal   = {Int J Mol Sci.},
  number    = {22(8)},
  pages     = {4050},
  abstract  = {Coculture systems employing adipose tissue-derived mesenchymal stromal/stem cells (ASC) and endothelial cells (EC) represent a widely used technique to model vascularization. Within this system, cell-cell communication is crucial for the achievement of functional vascular network formation. Extracellular vesicles (EVs) have recently emerged as key players in cell communication by transferring bioactive molecules between cells. In this study we aimed to address the role of EVs in ASC/EC cocultures by discriminating between cells, which have received functional EV cargo from cells that have not. Therefore, we employed the Cre-loxP system, which is based on donor cells expressing the Cre recombinase, whose mRNA was previously shown to be packaged into EVs and reporter cells containing a construct of floxed dsRed upstream of the eGFP coding sequence. The evaluation of Cre induced color switch in the reporter system via EVs indicated that there is no EV-mediated RNA transmission either between EC themselves or EC and ASC. However, since Cre mRNA was not found present in EVs, it remains unclear if Cre mRNA is generally not packaged into EVs or if EVs are not taken up by the utilized cell types. Our data indicate that this technique may not be applicable to evaluate EV-mediated cell-to-cell communication in an in vitro setting using EC and ASC. Further investigations will require a functional system showing efficient and specific loading of Cre mRNA or protein into EVs.},
  subject      = {Tissue Engineering},
  language  = {en}
}
@article{NuernbergerSchneidervanOschetal.,
  author    = {N{\"u}rnberger, Sylvia and Schneider, Cornelia and van Osch, Gerjo and Keibl, Claudia and Rieder, Bernhard and Monforte, Xavier and Teuschl, Andreas and M{\"u}hleder, Severin and Holnthoner, Wolfgang and Sch{\"a}dl, Barbara and Gahleitner, Christoph and Redl, Heinz and Wolbank, Susanne},
  title     = {Repopulation of an auricular cartilage scaffold, AuriScaff, perforated with an enzyme combination.},
  series = {Acta Biomaterialia},
  journal   = {Acta Biomaterialia},
  subject      = {Tissue Engineering},
  language  = {en}
}
@article{JohannesWeihsKarneretal.,
  author    = {Johannes, Hackethal and Weihs, Anna and Karner, Lisa and Metzger, Magdalena and Dungel, Peter and Hennerbichler, Simone and Redl, Heinz and Teuschl-Woller, Andreas Herbert},
  title     = {Novel Human Placenta-Based Extract for Vascularization Strategies in Tissue Engineering},
  series = {Tissue Eng Part C Methods},
  volume    = {27},
  journal   = {Tissue Eng Part C Methods},
  number    = {11},
  pages     = {616 -- 632},
  abstract  = {There is critical unmet need for new vascularized tissues to support or replace injured tissues and organs. Various synthetic and natural materials were already established for use of two-dimensional (2D) and three-dimensional (3D) in vitro neovascularization assays, however, they still cannot mimic the complex functions of the sum of the extracellular matrix (ECM) in native intact tissue. Currently, this issue is only addressed by artificial products such as Matrigel™, which comprises a complex mixture of ECM proteins, extracted from animal tumor tissue. Despite its outstanding bioactivity, the isolation from tumor tissue hinders its translation into clinical applications. Since nonhuman ECM proteins may cause immune reactions, as are frequently observed in clinical trials, human ECM proteins represent the best option when aiming for clinical applications. Here, we describe an effective method of isolating a human placenta substrate (hpS) that induces the spontaneous formation of an interconnected network of green fluorescence-labeled human umbilical vein endothelial cells (gfpHUVECs) in vitro. The substrate was biochemically characterized by using a combination of bicinchoninic acid (BCA) assay, DNA, and glycosaminoglycan (GAG) content assays, sodium dodecyl sulfate/polyacrylamide gel electrophoresis (SDS-PAGE) analysis and Western blot, angiogenesis arrays, chromatographic thrombin detection, high performance liquid chromatography (HPLC)-based amino acid quantification analysis, and assessment of antimicrobial properties. 2D in vitro cell culture experiments have been performed to determine the vasculogenic potential of hpS, which demonstrated that cell networks developed on hpS show a significantly higher degree of complexity (number of tubules/junctions; total/mean tube length) when compared with Matrigel. As 3D cell culture techniques represent a more accurate representation of the in vivo condition, the substrate was 3D solidified using various natural polymers. 3D in vitro vasculogenesis assays have been performed by seeding gfpHUVECs in an hpS-fibrinogen clot. In conclusion, hpS provides a potent human/material-based alternative to xenogenic-material-based biomaterials for vascularization strategies in tissue engineering.},
  subject      = {Tissue Engineering},
  language  = {en}
}
@inproceedings{KneblMortonRedletal.,
  author    = {Knebl, Gerald and Morton, Tatjana J. and Redl, Heinz and R{\"u}nzler, Dominik},
  title     = {Mechanical stimulation of cells in 3-dimensional fibrin constructs using a bioreactor},
  series = {3. Forschungsforum der {\"o}sterreichischen Fachhochschulen / Fachhochschule K{\"a}rnten},
  booktitle = {3. Forschungsforum der {\"o}sterreichischen Fachhochschulen / Fachhochschule K{\"a}rnten},
  pages     = {492 -- 493},
  subject      = {Cells},
  language  = {en}
}
@article{SchneiderRohringerKapelleretal.,
  author    = {Schneider, Karl H. and Rohringer, Sabrina and Kapeller, Barbara and Grasl, Christian and Kiss, Herbert and Heber, Stefan and Walter, Ingrid and Teuschl, Andreas H. and Podesser, Bruno K. and Bergmeister, Helga},
  title     = {Riboflavin-mediated photooxidation to improve the characteristics of decellularized human arterial small diameter vascular grafts},
  series = {Acta Biomaterialia},
  volume    = {116},
  journal   = {Acta Biomaterialia},
  pages     = {246 -- 258},
  abstract  = {Vascular grafts with a diameter of less than 6 mm are made from a variety of materials and techniques to provide alternatives to autologous vascular grafts. Decellularized materials have been proposed as a possible approach to create extracellular matrix (ECM) vascular prostheses as they are naturally derived and inherently support various cell functions. However, these desirable graft characteristics may be limited by alterations of the ECM during the decellularization process leading to decreased biomechanical properties and hemocompatibility. In this study, arteries from the human placenta chorion were decellularized using two distinct detergents (Triton X-100 or SDS), which differently affect ECM ultrastructure. To overcome biomechanical strength loss and collagen fiber exposure after decellularization, riboflavin-mediated UV (RUV) crosslinking was used to uniformly crosslink the collagenous ECM of the grafts. Graft characteristics and biocompatibility with and without RUV crosslinking were studied in vitro and in vivo. RUV-crosslinked ECM grafts showed significantly improved mechanical strength and smoothening of the luminal graft surfaces. Cell seeding using human endothelial cells revealed no cytotoxic effects of the RUV treatment. Short-term aortic implants in rats showed cell migration and differentiation of host cells. Functional graft remodeling was evident in all grafts. Thus, RUV crosslinking is a preferable tool to improve graft characteristics of decellularized matrix conduits.},
  subject      = {Tissue Engineering},
  language  = {en}
}
@article{HeinzelOberhauserKeibletal.,
  author    = {Heinzel, Johannes Christoph and Oberhauser, Viola and Keibl, Claudia and Sch{\"a}dl, Barbara and Swiadek, Nicole V. and L{\"a}ngle, Gregor and Frick, Helen and Slezak, Cyrill and Prahm, Cosima and Grillari, Johannes and Kolbenschlag, Jonas and Hercher, David},
  title     = {ESWT Diminishes Axonal Regeneration following Repair of the Rat Median Nerve with Muscle-In-Vein Conduits but Not after Autologous Nerve Grafting},
  series = {Biomedicines},
  volume    = {2022},
  journal   = {Biomedicines},
  number    = {10(8)},
  pages     = {1777},
  abstract  = {Investigations reporting positive effects of extracorporeal shockwave therapy (ESWT) on nerve regeneration are limited to the rat sciatic nerve model. The effects of ESWT on muscle-in-vein conduits (MVCs) have also not been investigated yet. This study aimed to evaluate the effects of ESWT after repair of the rat median nerve with either autografts (ANGs) or MVCs. In male Lewis rats, a 7 mm segment of the right median nerve was reconstructed either with an ANG or an MVC. For each reconstructive technique, one group of animals received one application of ESWT while the other rats served as controls. The animals were observed for 12 weeks, and nerve regeneration was assessed using computerized gait analysis, the grasping test, electrophysiological evaluations and histological quantification of axons, blood vessels and lymphatic vasculature. Here, we provide for the first time a comprehensive analysis of ESWT effects on nerve regeneration in a rat model of median nerve injury. Furthermore, this study is among the first reporting the quantification of lymphatic vessels following peripheral nerve injury and reconstruction in vivo. While we found no significant direct positive effects of ESWT on peripheral nerve regeneration, results following nerve repair with MVCs were significantly inferior to those after ANG repair.},
  subject      = {Tissue Engineering},
  language  = {en}
}
@article{VerstraetenHolnthonerVanSteenseletal.,
  author    = {Verstraeten, Valerie and Holnthoner, Wolfgang and Van Steensel, Maurice and Veraart, Joep C J M and Bladergroen, Reno S and Heckmann, Caronline A and Keskitalo, Salla and Frank, Jorge and Alitalo, Kari and van Geel, Michel and Steijlen, Peter M},
  title     = {Functional analysis of FLT4 mutations associated with Nonne-Milroy lymphedema},
  series = {Journal of Investigative Dermatology},
  journal   = {Journal of Investigative Dermatology},
  number    = {129(2)},
  pages     = {509 -- 512},
  subject      = {Nonne-Milroy iymphedema},
  language  = {en}
}
@article{Holnthoner,
  author    = {Holnthoner, Wolfgang},
  title     = {Adipose-tissue-derived therapeutic cells in their natural environment as an autologous cell therapy strategy: the microtissue-stromal vascular fraction},
  series = {Eur Cell Mater.},
  volume    = {2019},
  journal   = {Eur Cell Mater.},
  number    = {Feb, 37},
  pages     = {113 -- 133},
  abstract  = {The prerequisite for a successful clinical use of autologous adipose-tissue-derived cells is the highest possible regenerative potential of the applied cell population, the stromal vascular fraction (SVF). Current isolation methods depend on high enzyme concentration, lysis buffer, long incubation steps and mechanical stress, resulting in single cell dissociation. The aim of the study was to limit cell manipulation and obtain a derivative comprising therapeutic cells (microtissue-SVF) without dissociation from their natural extracellular matrix, by employing a gentle good manufacturing practice (GMP)-grade isolation. The microtissue-SVF yielded larger numbers of viable cells as compared to the improved standard-SVF, both with low enzyme concentration and minimal dead cell content. It comprised stromal tissue compounds (collagen, glycosaminoglycans, fibroblasts), capillaries and vessel structures (CD31+, smooth muscle actin+). A broad range of cell types was identified by surface-marker characterisation, including mesenchymal, haematopoietic, pericytic, blood and lymphatic vascular and epithelial cells. Subpopulations such as supra-adventitial adipose-derived stromal/stem cells and endothelial progenitor cells were significantly more abundant in the microtissue-SVF, corroborated by significantly higher potency for angiogenic tube-like structure formation in vitro. The microtissue-SVF showed the characteristic phenotype and tri-lineage mesenchymal differentiation potential in vitro and an immunomodulatory and pro-angiogenic secretome. In vivo implantation of the microtissue-SVF combined with fat demonstrated successful graft integration in nude mice. The present study demonstrated a fast and gentle isolation by minor manipulation of liposuction material, achieving a therapeutically relevant cell population with high vascularisation potential and immunomodulatory properties still embedded in a fraction of its original matrix.},
  subject      = {Adipose-tissue},
  language  = {en}
}
@article{SchneiderAignerHolnthoneretal.,
  author    = {Schneider, Karl Heinrich and Aigner, Petra and Holnthoner, Wolfgang and Monforte Vila, Xavier and N{\"u}rnberger, Sylvia and R{\"u}nzler, Dominik and Redl, Heinz and Teuschl, Andreas},
  title     = {Decellularized human placenta chorion matrix as a favorable source of small-diameter vascular grafts},
  series = {Acta Biomaterialia},
  journal   = {Acta Biomaterialia},
  subject      = {Grafting},
  language  = {en}
}
@article{HeimelSwiadekSlezaketal.,
  author    = {Heimel, Patrick and Swiadek, Nicole V. and Slezak, Paul and Kerbl, Markus and Schneider, Cornelia and N{\"u}rnberger, Sylvia and Redl, Heinz and Teuschl, Andreas and Hercher, David},
  title     = {Iodine-Enhanced Micro-CT Imaging of Soft Tissue on the Example of Peripheral Nerve Regeneration},
  series = {Contrast Media \& Molecular Imaging},
  journal   = {Contrast Media \& Molecular Imaging},
  subject      = {µCT},
  language  = {en}
}
@article{StrohmeierHofmannJacaketal.,
  author    = {Strohmeier, Karin and Hofmann, Martina and Jacak, Jaroslaw and Narzt, Marie-Sophie and Wahlmueller, Marlene and Mairhofer, Mario and Sch{\"a}dl, Barbara and Holnthoner, Wolfgang and Barsch, Martin and Sandhofer, Matthias and Wolbank, Susanne and Priglinger, Eleni},
  title     = {Multi-Level Analysis of Adipose Tissue Reveals the Relevance of Perivascular Subpopulations and an Increased Endothelial Permeability in Early-Stage Lipedema},
  series = {Biomedicines},
  volume    = {2022},
  journal   = {Biomedicines},
  number    = {10(5)},
  pages     = {1163},
  abstract  = {Lipedema is a chronic, progressive disease of adipose tissue with unknown etiology. Based on the relevance of the stromal vascular fraction (SVF) cell population in lipedema, we performed a thorough characterization of subcutaneous adipose tissue, SVF isolated thereof and the sorted populations of endothelial cells (EC), pericytes and cultured adipose-derived stromal/stem cells (ASC) of early-stage lipedema patients. We employed histological and gene expression analysis and investigated the endothelial barrier by immunofluorescence and analysis of endothelial permeability in vitro. Although there were no significant differences in histological stainings, we found altered gene expression of factors relevant for local estrogen metabolism (aromatase), preadipocyte commitment (ZNF423) and immune cell infiltration (CD11c) in lipedema on the tissue level, as well as in distinct cellular subpopulations. Machine learning analysis of immunofluorescence images of CD31 and ZO-1 revealed a morphological difference in the cellular junctions of EC cultures derived from healthy and lipedema individuals. Furthermore, the secretome of lipedema-derived SVF cells was sufficient to significantly increase leakiness of healthy human primary EC, which was also reflected by decreased mRNA expression of VE-cadherin. Here, we showed for the first time that the secretome of SVF cells creates an environment that triggers endothelial barrier dysfunction in early-stage lipedema. Moreover, since alterations in gene expression were detected on the cellular and/or tissue level, the choice of sample material is of high importance in elucidating this complex disease.},
  subject      = {Tissue Engineering},
  language  = {en}
}
@article{RohringerHolnthonerHackletal.,
  author    = {Rohringer, Sabrina and Holnthoner, Wolfgang and Hackl, Matthias and Weihs, Anna and R{\"u}nzler, Dominik and Skalicky, Susanna and Karbiener, Michael and Scheideler, Marcel and Pr{\"o}ll, Johannes and Gabriel, Christian and Schweighofer, Bernhard and Gr{\"o}ger, Marion and Spittler, Andreas and Grillari, Johannes and Redl, Heinz},
  title     = {Molecular and cellular effects of in vitro shockwave treatment on lymphatic endothelial cells.},
  series = {PLoS one},
  journal   = {PLoS one},
  subject      = {Shockwave},
  language  = {en}
}
@inproceedings{BayerRankTraxleretal.,
  author    = {Bayer, Natascha and Rank, Elisabet and Traxler, Lukas and Beckert, Erik and Drauschke, Andreas},
  title     = {Implementation of a capsular bag model to enable sufficient lens stabilization within a mechanical eye model},
  series = {Proc.SPIE 9307, Ophthalmic Technologies XXV},
  booktitle = {Proc.SPIE 9307, Ophthalmic Technologies XXV},
  subject      = {Mechanical Eye},
  language  = {en}
}
@article{HolnthonerSzwarc,
  author    = {Holnthoner, Wolfgang and Szwarc, Dorota},
  title     = {Purinergic P2Y 2 receptors modulate endothelial sprouting},
  series = {Cell Mol Life Sci.},
  volume    = {2020},
  journal   = {Cell Mol Life Sci.},
  number    = {Mar.},
  pages     = {885 -- 901},
  abstract  = {Purinergic P2 receptors are critical regulators of several functions within the vascular system, including platelet aggregation, vascular inflammation, and vascular tone. However, a role for ATP release and P2Y receptor signalling in angiogenesis remains poorly defined. Here, we demonstrate that blood vessel growth is controlled by P2Y2 receptors. Endothelial sprouting and vascular tube formation were significantly dependent on P2Y2 expression and inhibition of P2Y2 using a selective antagonist blocked microvascular network generation. Mechanistically, overexpression of P2Y2 in endothelial cells induced the expression of the proangiogenic molecules CXCR4, CD34, and angiopoietin-2, while expression of VEGFR-2 was decreased. Interestingly, elevated P2Y2 expression caused constitutive phosphorylation of ERK1/2 and VEGFR-2. However, stimulation of cells with the P2Y2 agonist UTP did not influence sprouting unless P2Y2 was constitutively expressed. Finally, inhibition of VEGFR-2 impaired spontaneous vascular network formation induced by P2Y2 overexpression. Our data suggest that P2Y2 receptors have an essential function in angiogenesis, and that P2Y2 receptors present a therapeutic target to regulate blood vessel growth.},
  subject      = {Angiogenesis},
  language  = {en}
}
@inproceedings{RankTraxlerBayeretal.,
  author    = {Rank, Elisabet and Traxler, Lukas and Bayer, Natascha and Reutterer, Bernd and Lux, Kirsten and Drauschke, Andreas},
  title     = {Reproducibility analysis of measurements with a mechanical semiautomatic eye model for evaluation of intraocular lenses},
  series = {Proc.SPIE 8936, Design and Quality for Biomedical Technologies VII},
  booktitle = {Proc.SPIE 8936, Design and Quality for Biomedical Technologies VII},
  subject      = {Mechanical Eye},
  language  = {en}
}
@article{TriskoFleckKauetal.,
  author    = {Trisko, Johanna and Fleck, Johanna and Kau, Silvio and Oesterreicher, Johannes and Holnthoner, Wolfgang},
  title     = {Lymphatic and Blood Endothelial Extracellular Vesicles: A Story Yet to Be Written},
  series = {Life},
  volume    = {2022},
  journal   = {Life},
  number    = {12(5)},
  pages     = {654},
  abstract  = {Extracellular vesicles (EVs), such as exosomes, microvesicles, and apoptotic bodies, are cell-derived, lipid bilayer-enclosed particles mediating intercellular communication and are therefore vital for transmitting a plethora of biological signals. The vascular endothelium substantially contributes to the circulating particulate secretome, targeting important signaling pathways that affect blood cells and regulate adaptation and plasticity of endothelial cells in a paracrine manner. Different molecular signatures and functional properties of endothelial cells reflect their heterogeneity among different vascular beds and drive current research to understand varying physiological and pathological effects of blood and lymphatic endothelial EVs. Endothelial EVs have been linked to the development and progression of various vascular diseases, thus having the potential to serve as biomarkers and clinical treatment targets. This review aims to provide a brief overview of the human vasculature, the biology of extracellular vesicles, and the current knowledge of endothelium-derived EVs, including their potential role as biomarkers in disease development.},
  subject      = {Tissue Engineering},
  language  = {en}
}
@article{FernerOrtnerBleckmannHuberGriesPavkovKelleretal.,
  author    = {Ferner-Ortner-Bleckmann, Judith and Huber-Gries, Carina and Pavkov-Keller, Tea and Keller, Walter and Mader, Christoph and Ilk, Nicola and Sleytr, Uwe B. and Egelseer, Eva-Maria},
  title     = {The high-molecular-mass amylase (HMMA) of Geobacillus stearothermophilus ATCC 12980 interacts with the cell wall components by virtue of three specific binding regions},
  series = {Molecular Microbiology},
  journal   = {Molecular Microbiology},
  number    = {72(6)},
  pages     = {1448 -- 1461},
  subject      = {Cells},
  language  = {en}
}
@misc{LiousiaSalzerMandtetal.,
  author    = {Liousia, Varvara and Salzer, Elias and Mandt, Denise and R{\"u}nzler, Dominik},
  title     = {The impact of ethanol in vitro and in vivo: a comparative study},
  subject      = {In Vivo},
  language  = {en}
}
@article{GhalamkarpourHolnthonerSaharinenetal.,
  author    = {Ghalamkarpour, A. and Holnthoner, Wolfgang and Saharinen, Pipsa and Boon, Laurence M and Mulliken, J B and Alitalo, Kari},
  title     = {Recessive primary congenital lymphoedema caused by a VEGFR3 mutation},
  series = {Journal of Medical Genetics},
  journal   = {Journal of Medical Genetics},
  number    = {46(6)},
  pages     = {399 -- 404},
  subject      = {Lymphoedema},
  language  = {en}
}
@article{SayerZandriniMarkovicetal.,
  author    = {Sayer, Simon and Zandrini, Tommaso and Markovic, Marica and Van Hoorick, Jasper and Van Vlierberghe, Sandra and Baudis, Stefan and Holnthoner, Wolfgang and Ovsianikov, Aleksandr},
  title     = {Guiding cell migration in 3D with high-resolution photografting},
  series = {Scientific Reports},
  volume    = {2022},
  journal   = {Scientific Reports},
  number    = {12(1), 10196},
  pages     = {8626},
  abstract  = {Multi-photon lithography (MPL) has proven to be a suitable tool to precisely control the microenvironment of cells in terms of the biochemical and biophysical properties of the hydrogel matrix. In this work, we present a novel method, based on multi-photon photografting of 4,4′-diazido-2,2′-stilbenedisulfonic acid (DSSA), and its capabilities to induce cell alignment, directional cell migration and endothelial sprouting in a gelatin-based hydrogel matrix. DSSA-photografting allows for the fabrication of complex patterns at a high-resolution and is a biocompatible, universally applicable and straightforward process that is comparably fast. We have demonstrated the preferential orientation of human adipose-derived stem cells (hASCs) in response to a photografted pattern. Co-culture spheroids of hASCs and human umbilical vein endothelial cells (HUVECs) have been utilized to study the directional migration of hASCs into the modified regions. Subsequently, we have highlighted the dependence of endothelial sprouting on the presence of hASCs and demonstrated the potential of photografting to control the direction of the sprouts. MPL-induced DSSA-photografting has been established as a promising method to selectively alter the microenvironment of cells.},
  subject      = {Tissue Engineering},
  language  = {en}
}
@article{HromadaHartmannOesterreicheretal.,
  author    = {Hromada, Carina and Hartmann, Jaana and Oesterreicher, Johannes and Stoiber, Anton and Daerr, Anna and Sch{\"a}dl, Barbara and Priglinger, Eleni and Teuschl-Woller, Andreas H. and Holnthoner, Wolfgang and Heinzel, Johannes Christoph and Hercher, David},
  title     = {Occurrence of Lymphangiogenesis in Peripheral Nerve Autografts Contrasts Schwann Cell-Induced Apoptosis of Lymphatic Endothelial Cells In Vitro},
  series = {Biomolecules},
  volume    = {2022},
  journal   = {Biomolecules},
  number    = {12, 6},
  pages     = {820},
  abstract  = {Peripheral nerve injuries pose a major clinical concern world-wide, and functional recovery after segmental peripheral nerve injury is often unsatisfactory, even in cases of autografting. Although it is well established that angiogenesis plays a pivotal role during nerve regeneration, the influence of lymphangiogenesis is strongly under-investigated. In this study, we analyzed the presence of lymphatic vasculature in healthy and regenerated murine peripheral nerves, revealing that nerve autografts contained increased numbers of lymphatic vessels after segmental damage. This led us to elucidate the interaction between lymphatic endothelial cells (LECs) and Schwann cells (SCs) in vitro. We show that SC and LEC secretomes did not influence the respective other cell types' migration and proliferation in 2D scratch assay experiments. Furthermore, we successfully created lymphatic microvascular structures in SC-embedded 3D fibrin hydrogels, in the presence of supporting cells; whereas SCs seemed to exert anti-lymphangiogenic effects when cultured with LECs alone. Here, we describe, for the first time, increased lymphangiogenesis after peripheral nerve injury and repair. Furthermore, our findings indicate a potential lymph-repellent property of SCs, thereby providing a possible explanation for the lack of lymphatic vessels in the healthy endoneurium. Our results highlight the importance of elucidating the molecular mechanisms of SC-LEC interaction.},
  subject      = {Tissue Engineering},
  language  = {en}
}
@article{LauerPrahmThieletal.,
  author    = {Lauer, Henrik and Prahm, Cosima and Thiel, Johannes Tobias and Kolbenschlag, Jonas and Daigeler, Adrien and Hercher, David and Heinzel, Johannes Christoph},
  title     = {The Grasping Test Revisited: A Systematic Review of Functional Recovery in Rat Models of Median Nerve Injury},
  series = {Biomedicines},
  volume    = {2022},
  journal   = {Biomedicines},
  number    = {10(8)},
  pages     = {1878},
  abstract  = {The rat median nerve model is a well-established and frequently used model for peripheral nerve injury and repair. The grasping test is the gold-standard to evaluate functional recovery in this model. However, no comprehensive review exists to summarize the course of functional recovery in regard to the lesion type. According to PRISMA-guidelines, research was performed, including the databases PubMed and Web of Science. Groups were: (1) crush injury, (2) transection with end-to-end or with (3) end-to-side coaptation and (4) isogenic or acellular allogenic grafting. Total and respective number, as well as rat strain, type of nerve defect, length of isogenic or acellular allogenic allografts, time at first signs of motor recovery (FSR) and maximal recovery grasping strength (MRGS), were evaluated. In total, 47 articles met the inclusion criteria. Group I showed earliest signs of motor recovery. Slow recovery was observable in group III and in graft length above 25 mm. Isografts recovered faster compared to other grafts. The onset and course of recovery is heavily dependent from the type of nerve injury. The grasping test should be used complementary in addition to other volitional and non-volitional tests. Repetitive examinations should be planned carefully to optimize assessment of valid and reliable data.},
  subject      = {Tissue Engineering},
  language  = {en}
}
@article{RothbauerByrneSchobesbergeretal.,
  author    = {Rothbauer, Mario and Byrne, Ruth A. and Schobesberger, Silvia and Olmos Calvo, Isabel and Fischer, Anita and Reihs, Eva I. and Spitz, Sarah and Bachmann, Barbara and Sevelda, Florian and Holinka, Johannes and Holnthoner, Wolfgang and Redl, Heinz and Toegel, Stefan and Windhager, Reinhard and Kiener, Hans P. and Ertl, Peter},
  title     = {Establishment of a human three-dimensional chip-based chondro-synovial coculture joint model for reciprocal cross talk studies in arthritis research},
  series = {Lab on a Chip},
  volume    = {2021},
  journal   = {Lab on a Chip},
  number    = {21},
  pages     = {4128 -- 4143},
  abstract  = {Rheumatoid arthritis is characterised by a progressive, intermittent inflammation at the synovial membrane, which ultimately leads to the destruction of the synovial joint. The synovial membrane as the joint capsule's inner layer is lined with fibroblast-like synoviocytes that are the key player supporting persistent arthritis leading to bone erosion and cartilage destruction. While microfluidic models that model molecular aspects of bone erosion between bone-derived cells and synoviocytes have been established, RA's synovial-chondral axis has not yet been realised using a microfluidic 3D model based on human patient in vitro cultures. Consequently, we established a chip-based three-dimensional tissue coculture model that simulates the reciprocal cross talk between individual synovial and chondral organoids. When co-cultivated with synovial organoids, we could demonstrate that chondral organoids induce a higher degree of cartilage physiology and architecture and show differential cytokine response compared to their respective monocultures highlighting the importance of reciprocal tissue-level cross talk in the modelling of arthritic diseases.},
  subject      = {Tissue Engineering},
  language  = {en}
}
@misc{LiousiaRuenzler,
  author    = {Liousia, Varvara and R{\"u}nzler, Dominik},
  title     = {Stage- and dose-dependent effects of methanol and ethanol on the locomotor activity of zebrafish larvae},
  subject      = {Locomotor Activity},
  language  = {en}
}
@article{KierspelKadekBarranetal.,
  author    = {Kierspel, Thomas and Kadek, Alan and Barran, Perdita and Bellina, Bruno and Bijedic, Adi and Brodmerkel, Maxim N. and Commandeur, Jan and Caleman, Carl and Damjanovic, Tomislav and Dawod, Ibrahim and De Santis, Emiliano and Lekkas, Alexandros and Lorenzen, Kristina and L{\´o}pez Morillo, Luis and Mandl, Thomas and Marklund, Erik G. and Papanastasiou, Dimitris and Ramakers, Lennart A. I. and Schweikhard, Lutz and Simke, Florian and Sinelnikova, Anna and Smyrnakis, Athanasios and Timneanu, Nicusor and Uetrecht, Charlotte},
  title     = {Coherent diffractive imaging of proteins and viral capsids: simulating MS SPIDOC},
  series = {Analytical and Bioanalytical Chemistry},
  volume    = {2023},
  journal   = {Analytical and Bioanalytical Chemistry},
  number    = {415},
  doi       = {https://doi.org/10.1007/s00216-023-04658-y},
  pages     = {4209 -- 4220},
  abstract  = {MS SPIDOC is a novel sample delivery system designed for single (isolated) particle imaging at X-ray Free-Electron Lasers that is adaptable towards most large-scale facility beamlines. Biological samples can range from small proteins to MDa particles. Following nano-electrospray ionization, ionic samples can be m/z-filtered and structurally separated before being oriented at the interaction zone. Here, we present the simulation package developed alongside this prototype. The first part describes how the front-to-end ion trajectory simulations have been conducted. Highlighted is a quadrant lens; a simple but efficient device that steers the ion beam within the vicinity of the strong DC orientation field in the interaction zone to ensure spatial overlap with the X-rays. The second part focuses on protein orientation and discusses its potential with respect to diffractive imaging methods. Last, coherent diffractive imaging of prototypical T = 1 and T = 3 norovirus capsids is shown. We use realistic experimental parameters from the SPB/SFX instrument at the European XFEL to demonstrate that low- resolution diffractive imaging data (q < 0.3 nm -1 ) can be collected with only a few X-ray pulses. Such low-resolution data are sufficient to distinguish between both symmetries of the capsids, allowing to probe low abundant species in a beam if MS SPIDOC is used as sample delivery.},
  subject      = {SPI},
  language  = {en}
}
@misc{FriedrichLakicPraehauseretal.,
  author    = {Friedrich, Robin and Lakic, Nevana and Pr{\"a}hauser, Linda and Schweitzer, Karoline and Olscher, Christoph and Monforte Vila, Xavier and Leitner, Rita and Gepp, Barbara},
  title     = {Effects of Plastic on the Freshwater Snail Biomphalaria Glabrata},
  series = {SETAC Europe 32nd Annual Meeting in Copenhagen, Denmark from 15 - 19. May 2022},
  journal   = {SETAC Europe 32nd Annual Meeting in Copenhagen, Denmark from 15 - 19. May 2022},
  subject      = {Ecotoxicology},
  language  = {en}
}
@article{AshmwePosaRuehrnoessletal.,
  author    = {Ashmwe, Mohamed and Posa, Katja and R{\"u}hrn{\"o}ßl, Alexander and Heinzel, Johannes Christoph and Heimel, Patrick and Mock, Michael and Sch{\"a}dl, Barbara and Keibl, Claudia and Couillard-Despres, Sebastien and Redl, Heinz and Mittermayr, Rainer and Hercher, David},
  title     = {Effects of Extracorporeal Shockwave Therapy on Functional Recovery and Circulating miR-375 and miR-382-5p after Subacute and Chronic Spinal Cord Contusion Injury in Rats},
  series = {Biomedicines},
  volume    = {2022},
  journal   = {Biomedicines},
  number    = {10(7)},
  doi       = {https://doi.org/10.3390/biomedicines10071630},
  pages     = {1630},
  abstract  = {Extracorporeal shockwave therapy (ESWT) can stimulate processes to promote regeneration, including cell proliferation and modulation of inflammation. Specific miRNA expression panels have been established to define correlations with regulatory targets within these pathways. This study aims to investigate the influence of low-energy ESWT-applied within the subacute and chronic phase of SCI (spinal cord injury) on recovery in a rat spinal cord contusion model. Outcomes were evaluated by gait analysis, µCT and histological analysis of spinal cords. A panel of serum-derived miRNAs after SCI and after ESWT was investigated to identify injury-, regeneration- and treatment-associated expression patterns. Rats receiving ESWT showed significant improvement in motor function in both a subacute and a chronic experimental setting. This effect was not reflected in changes in morphology, µCT-parameters or histological markers after ESWT. Expression analysis of various miRNAs, however, revealed changes after SCI and ESWT, with increased miR-375, indicating a neuroprotective effect, and decreased miR-382-5p potentially improving neuroplasticity via its regulatory involvement with BDNF. We were able to demonstrate a functional improvement of ESWT-treated animals after SCI in a subacute and chronic setting. Furthermore, the identification of miR-375 and miR-382-5p could potentially provide new targets for therapeutic intervention in future studies.},
  subject      = {Tissue Engineering},
  language  = {en}
}