@article{MaleinerTomaschHeheretal., author = {Maleiner, Babette and Tomasch, Janine and Heher, Philipp and Spadiut, Oliver and R{\"u}nzler, Dominik and Fuchs, Christiane}, title = {The Importance of Biophysical and Biochemical Stimuli in Dynamic Skeletal Muscle Models.}, series = {Frontiers in Physiology}, journal = {Frontiers in Physiology}, abstract = {Classical approaches to engineer skeletal muscle tissue based on current regenerative and surgical procedures still do not meet the desired outcome for patient applications. Besides the evident need to create functional skeletal muscle tissue for the repair of volumetric muscle defects, there is also growing demand for platforms to study muscle-related diseases, such as muscular dystrophies or sarcopenia. Currently, numerous studies exist that have employed a variety of biomaterials, cell types and strategies for maturation of skeletal muscle tissue in 2D and 3D environments. However, researchers are just at the beginning of understanding the impact of different culture settings and their biochemical (growth factors and chemical changes) and biophysical cues (mechanical properties) on myogenesis. With this review we intend to emphasize the need for new in vitro skeletal muscle (disease) models to better recapitulate important structural and functional aspects of muscle development. We highlight the importance of choosing appropriate system components, e.g., cell and biomaterial type, structural and mechanical matrix properties or culture format, and how understanding their interplay will enable researchers to create optimized platforms to investigate myogenesis in healthy and diseased tissue. Thus, we aim to deliver guidelines for experimental designs to allow estimation of the potential influence of the selected skeletal muscle tissue engineering setup on the myogenic outcome prior to their implementation. Moreover, we offer a workflow to facilitate identifying and selecting different analytical tools to demonstrate the successful creation of functional skeletal muscle tissue. Ultimately, a refinement of existing strategies will lead to further progression in understanding important aspects of muscle diseases, muscle aging and muscle regeneration to improve quality of life of patients and enable the establishment of new treatment options.}, subject = {Bioreactor}, language = {en} } @misc{HeherTomaschMaleineretal., author = {Heher, Philipp and Tomasch, Janine and Maleiner, Babette and Redl, Heinz and Fuchs, Christiane}, title = {The Importance of Biomechanical Cues for In Vitro Skeletal Myogenesis}, subject = {In Vitro}, language = {en} } @inproceedings{MeyerDonsaTruskalleretal., author = {Meyer, Markus and Donsa, Klaus and Truskaller, Thomas and Frohner, Matthias and Pohn, Birgit and Felfernig, Alexander and Sinner, Franz and Pieber, Thomas}, title = {Development of a Protocol for Automated Glucose Measurement Transmission Used in Clinical Decision Support Systems Based on the Continua Design Guidelines}, series = {Conference Proceedings eHealth 2018}, booktitle = {Conference Proceedings eHealth 2018}, subject = {Mobile Health}, language = {en} } @misc{MeyerDonsaTruskalleretal., author = {Meyer, Markus and Donsa, Klaus and Truskaller, Thomas and Frohner, Matthias and Pohn, Birgit and Felfernig, Alexander and Sinner, Franz and Pieber, Thomas}, title = {Development of a Protocol for Automated Glucose Measurement Transmission Used in Clinical Decision Support Systems Based on the Continua Design Guidelines}, subject = {Mobile Health}, language = {en} } @article{SchandaKeiblHeimeletal., author = {Schanda, Jakob and Keibl, Claudia and Heimel, Patrick and Monforte, Xavier and Feichtinger, Xaver and Teuschl, Andreas and Baierl, Andreas and Muschitz, Christian and Redl, Heinz and Fialka, Christian and Mittermayr, Rainer}, title = {Zoledronic Acid Substantially Improves Bone Microarchitecture and Biomechanical Properties After Rotator Cuff Repair in a Rodent Chronic Defect Model}, series = {Am J Sports Med}, volume = {2020 Jul}, journal = {Am J Sports Med}, number = {48 (9)}, pages = {2151 -- 2160}, abstract = {Background: Bone mineral density at the humeral head is reduced in patients with chronic rotator cuff tears. Bone loss in the humeral head is associated with repair failure after rotator cuff reconstruction. Bisphosphonates (eg, zoledronic acid) increase bone mineral density. Hypothesis: Zoledronic acid improves bone mineral density of the humeral head and biomechanical properties of the enthesis after reconstruction of chronic rotator cuff tears in rats. Study design: Controlled laboratory study. Methods: A total of 32 male Sprague-Dawley rats underwent unilateral (left) supraspinatus tenotomy with delayed transosseous rotator cuff reconstruction after 3 weeks. All rats were sacrificed 8 weeks after rotator cuff repair. Animals were randomly assigned to 1 of 2 groups. At 1 day after rotator cuff reconstruction, the intervention group was treated with a single subcutaneous dose of zoledronic acid at 100 µg/kg bodyweight, and the control group received 1 mL of subcutaneous saline solution. In 12 animals of each group, micro-computed tomography scans of both shoulders were performed as well as biomechanical testing of the supraspinatus enthesis of both sides. In 4 animals of each group, histological analyses were conducted. Results: In the intervention group, bone volume fraction (bone volume/total volume [BV/TV]) of the operated side was higher at the lateral humeral head (P = .005) and the medial humeral head (P = .010) compared with the control group. Trabecular number on the operated side was higher at the lateral humeral head (P = .004) and the medial humeral head (P = .001) in the intervention group. Maximum load to failure rates on the operated side were higher in the intervention group (P < .001). Cortical thickness positively correlated with higher maximum load to failure rates in the intervention group (r = 0.69; P = .026). Histological assessment revealed increased bone formation in the intervention group. Conclusion: Single-dose therapy of zoledronic acid provided an improvement of bone microarchitecture at the humeral head as well as an increase of maximum load to failure rates after transosseous reconstruction of chronic rotator cuff lesions in rats. Clinical relevance: Zoledronic acid improves bone microarchitecture as well as biomechanical properties after reconstruction of chronic rotator cuff tears in rodents. These results need to be verified in clinical investigations.}, subject = {Tissue Engineering}, language = {en} } @article{SlezakSlezakHartingeretal., author = {Slezak, Paul and Slezak, Cyrill and Hartinger, Joachim and Teuschl, Andreas and N{\"u}rnberger, Sylvia and Redl, Heinz and Mittermayr, Rainer}, title = {A Low Cost Implantation Model in the Rat That Allows a Spatial Assessment of Angiogenesis.}, series = {Frontiers in Bioengineering and Biotechnology}, journal = {Frontiers in Bioengineering and Biotechnology}, abstract = {There is continual demand for animal models that allow a quantitative assessment of angiogenic properties of biomaterials, therapies, and pharmaceuticals. In its simplest form, this is done by subcutaneous material implantation and subsequent vessel counting which usually omits spatial data. We have refined an implantation model and paired it with a computational analytic routine which outputs not only vessel count but also vessel density, distribution, and vessel penetration depth, that relies on a centric vessel as a reference point. We have successfully validated our model by characterizing the angiogenic potential of a fibrin matrix in conjunction with recombinant human vascular endothelial growth factor (rhVEGF165). The inferior epigastric vascular pedicles of rats were sheathed with silicone tubes, which were subsequently filled with 0.2 ml of fibrin and different doses of rhVEGF165, centrically embedding the vessels. Over 4 weeks, tissue samples were harvested and subsequently immunohistologically stained and computationally analyzed. The model was able to detect variations over the angiogenic potentials of growth factor spiked fibrin matrices. Adding 20 ng of rhVEGF165 resulted in a significant increase in vasculature while 200 ng of rhVEGF165 did not improve vascular growth. Vascularized tissue volume increased during the first week and vascular density increased during the second week. Total vessel count increased significantly and exhibited a peak after 2 weeks which was followed by a resorption of vasculature by week 4. In summary, a simple implantation model to study in vivo vascularization with only a minimal workload attached was enhanced to include morphologic data of the emerging vascular tree.}, subject = {Tissue Engineering}, language = {en} } @article{BerkovitchCohenPeledetal., author = {Berkovitch, Yulia and Cohen, Talia and Peled, Eli and Schmidhammer, Robert and Hildner, Florian and Teuschl, Andreas and Wolbank, Susanne and Yelin, Dvir and Redl, Heinz and Seliktar, Dror}, title = {Hydrogel composition and laser micropatterning to regulate sciatic nerve regeneration.}, series = {Journal of Tissue Engineering and Regenerative Medicine}, journal = {Journal of Tissue Engineering and Regenerative Medicine}, pages = {1049 -- 1061}, abstract = {Treatment of peripheral nerve injuries has evolved over the past several decades to include the use of sophisticated new materials endowed with trophic and topographical cues that are essential for in vivo nerve fibre regeneration. In this research, we explored the use of an advanced design strategy for peripheral nerve repair, using biological and semi-synthetic hydrogels that enable controlled environmental stimuli to regenerate neurons and glial cells in a rat sciatic nerve resection model. The provisional nerve growth conduits were composed of either natural fibrin or adducts of synthetic polyethylene glycol and fibrinogen or gelatin. A photo-patterning technique was further applied to these 3D hydrogel biomaterials, in the form of laser-ablated microchannels, to provide contact guidance for unidirectional growth following sciatic nerve injury. We tested the regeneration capacity of subcritical nerve gap injuries in rats treated with photo-patterned materials and compared these with injuries treated with unpatterned hydrogels, either stiff or compliant. Among the factors tested were shear modulus, biological composition, and micropatterning of the materials. The microchannel guidance patterns, combined with appropriately matched degradation and stiffness properties of the material, proved most essential for the uniform tissue propagation during the nerve regeneration process.}, subject = {Tissue Engineering}, language = {en} } @article{NauTeuschlEbneretal., author = {Nau, Thomas and Teuschl, Andreas and Ebner, Anna and Jung, Ilse and Schenk, Christian}, title = {Low revision rate and excellent outcome of primary ACL repair with a minimum follow-up of 5 years.}, series = {Muscle, ligaments and tendons Journal}, journal = {Muscle, ligaments and tendons Journal}, pages = {185 -- 190}, abstract = {Introduction: Due to limitations of ACL reconstruction, primary ACL repair has recently regained research interest. Although abandoned in the past, primary repair with conservation of the original ligament demonstrates considerable advantages compared to reconstruction. We hypothesized that early repair, strictly limited to patients with a proximal ACL rupture and excellent tissue quality of the remaining ACL stump, would lead to equal revision rates and subjective outcomes as reported for ACL reconstruction after a minimum of 5 years. Methods: In this questionnaire study, patients who had a primary ACL repair between 2002 and 2009 were invited to participate. Besides any potential revision surgery, the Tegner activity scale and the Knee Injury and Osteoarthritis Outcome Score were included in the evaluation. Results: Out of 1912 patients who had ACL related surgery during the observation period, 221 (11.4\%) had a primary ACL repair. 60 patients (61 knees) were available for follow-up. In 2/61 (3.3\%) cases ACL revision surgery was performed and one patient had meniscus surgery of the affected side. The median Tegner activity scale was 6 (range, 3 to 10). The mean KOOS subscores were 88.8\% (Function/Sports), 86.6\% (Quality of life), 94.6 (Symptoms), 94.0 (Pain) and 97.0 (Activities of Daily Living). Conclusion: Primary ACL repair, strictly limited to proximal ruptures with good tissue quality leads to revision rates and subjective outcome comparable to ACL reconstruction. Level of evidence: IV.}, subject = {Regeneration}, language = {en} } @incollection{HackethalSchuhHoferetal., author = {Hackethal, Johannes and Schuh, Christina and Hofer, Alexandra and Meixner, Barbara and Hennerbichler, Simone and Redl, Heinz and Teuschl, Andreas}, title = {Human Placenta Laminin-111 as a Multifunctional Protein for Tissue Engineering and Regenerative Medicine}, series = {Advances in Experimental Medicine and Biology}, booktitle = {Advances in Experimental Medicine and Biology}, publisher = {Springer}, publisher = {Fachhochschule Technikum Wien}, subject = {Biomaterial}, language = {en} } @misc{Drauschke, author = {Drauschke, Andreas}, title = {Neues aus der Welt der Optik - von Laserschwertern und Tarnvorrichtungen}, subject = {Optics}, language = {de} } @misc{Drauschke, author = {Drauschke, Andreas}, title = {Effiziente Solarzellen mit Metamaterialien}, subject = {Photonics}, language = {de} } @inproceedings{TkachenkoBrilJagosDavidetal., author = {Tkachenko Bril, Andr{\´e}s Igor and Jagos, Harald and David, Veronika and Pils, Katharina and Gaudernak, Jakob and Rafolt, Dietmar}, title = {Proof of Concept of a Partial Weight-Bearing Supporting Real-Time Feedback System}, series = {Health Informatics meets eHealth - Proceedings of the 12th eHealth Conference}, booktitle = {Health Informatics meets eHealth - Proceedings of the 12th eHealth Conference}, editor = {Schreier, G{\"u}nter and Hayn, Dieter}, publisher = {IOS Press}, address = {Amsterdam}, subject = {Ambulation}, language = {en} } @inproceedings{FranzlReifBergeretal., author = {Franzl, Gerald and Reif, Valerie and Berger, Angela and Frohner, Matthias and Gottschalk, Marion and Koch, Georg}, title = {Interoperabilit{\"a}t im Datenaustausch in der Energiewirtschaft vom Use Case zum Test der Integrationsprofile}, series = {15. Symposium Energieinnovation}, booktitle = {15. Symposium Energieinnovation}, subject = {Interoperability}, language = {de} } @misc{FranzlReifBergeretal., author = {Franzl, Gerald and Reif, Valerie and Berger, Angela and Frohner, Matthias and Gottschalk, Marion and Koch, Georg}, title = {Interoperabilit{\"a}t im Datenaustausch in der Energiewirtschaft vom Use Case zum Test der Integrationsprofile}, subject = {Interoperability}, language = {de} } @misc{TkachenkoBrilJagosDavidetal., author = {Tkachenko Bril, Andr{\´e}s Igor and Jagos, Harald and David, Veronika and Pils, Katharina and Gaudernak, Jakob and Rafolt, Dietmar}, title = {Proof of Concept of a Partial Weight-Bearing Supporting Real-Time Feedback System}, subject = {Ambulation}, language = {en} } @misc{Drauschke, author = {Drauschke, Andreas}, title = {Applications of Optogenetics}, subject = {Optogenetics}, language = {en} } @misc{VeiglDavid, author = {Veigl, Christoph and David, Veronika}, title = {AsTeRICS und FlipMouse: Flexible Eingabesysteme und kosteng{\"u}nstige L{\"o}sungen f{\"u}r Kommunikation und Umgebungssteuerung f{\"u}r Menschen mit Behinderung}, subject = {Communication}, language = {de} } @techreport{Freistetter, author = {Freistetter, Florian}, title = {The Power of Lauf}, organization = {ORF Sendung "Science Busters"}, subject = {Bioreactor}, language = {de} } @article{BerkovitchCohenPeledetal., author = {Berkovitch, Yulia and Cohen, Talia and Peled, Eli and Schmidhammer, Robert and Hildner, Florian and Teuschl, Andreas and Wolbank, Susanne and Yelin, Dvir and Redl, Heinz and Seliktar, Dror}, title = {Hydrogel Composition and Laser Micro-Patterning to Regulate Sciatic Nerve Regeneration}, series = {Journal of Tissue Engineering and Regenerative Medicine}, volume = {12}, journal = {Journal of Tissue Engineering and Regenerative Medicine}, number = {4}, subject = {Micro-Patterning}, language = {en} } @misc{SaboEckelt, author = {Sabo, Anton and Eckelt, Markus}, title = {Running Shoes - Possible Correlations of Biomechanical and Material Tests}, subject = {Shoes}, language = {en} } @misc{Eckelt, author = {Eckelt, Markus}, title = {Customizing Shoe Insoles through CAD-FEM Modelling}, subject = {Customized Equipment}, language = {en} } @article{BernhardFergusonRiederetal., author = {Bernhard, Jonathan and Ferguson, James and Rieder, Bernhard and Heimel, Patrick and Nau, Thomas and Tangl, Stefan and Redl, Heinz and Vunjak-Novakovic, Gordana}, title = {Tissue-engineered hypertrophic chondrocyte grafts enhanced long bone repair biomaterials}, series = {Biomaterials}, journal = {Biomaterials}, number = {139}, pages = {202 -- 212}, subject = {Grafting}, language = {en} } @misc{Eckelt, author = {Eckelt, Markus}, title = {Evaluating the Interaction Between Rim and Brake Pad on a Test Bench}, subject = {Brake}, language = {en} } @article{HeherMaleinerPruelleretal., author = {Heher, Philipp and Maleiner, Babette and Pr{\"u}ller, Johanna and Teuschl, Andreas and Kollmitzer, Josef and Monforte Vila, Xavier and Wolbank, Susanne and Redl, Heinz and R{\"u}nzler, Dominik and Fuchs, Christiane}, title = {A novel bioreactor for the generation of highly aligned 3D skeletal muscle-like constructs through orientation of fibrin via application of static strain}, series = {Acta Biomaterialia}, journal = {Acta Biomaterialia}, subject = {Bioreactor}, language = {en} } @article{JagosDavidReicheletal., author = {Jagos, Harald and David, Veronika and Reichel, Martin and Kotzian, Stefan and Schlossarek, Stefan and Haller, Michael and Rafolt, Dietmar}, title = {Tele-monitoring of the rehabilitation progress in stroke patients}, series = {Studies in Health Technology and Informatics}, journal = {Studies in Health Technology and Informatics}, subject = {Rehabilitation}, language = {en} } @inproceedings{LitzenbergerChristensenHofstaetteretal., author = {Litzenberger, Stefan and Christensen, Troels and Hofst{\"a}tter, Otto and Sabo, Anton}, title = {Prediction of Road Surface Quality during Cycling Using Smartphone Accelerometer Data}, series = {12th Conference of the International Sports Engineering Association}, booktitle = {12th Conference of the International Sports Engineering Association}, subject = {Cycling}, language = {en} } @misc{SauermannDavidSchloegletal., author = {Sauermann, Stefan and David, Veronika and Schl{\"o}gl, Alois and Egelkraut, Reinhard and Frohner, Matthias and Pohn, Birgit and Urbauer, Philipp and Mense, Alexander}, title = {Biosignals, Standards and FHIR - The Way to Go?}, subject = {Biosignals}, language = {en} } @inproceedings{FrohnerMeyerDonsaetal., author = {Frohner, Matthias and Meyer, Markus and Donsa, Klaus and Urbauer, Philipp and David, Veronika and Sauermann, Stefan}, title = {Telemonitoring of Blood Glucose - A Prototype Android Application Enhancing the Patient / Health Professional Experience Using Health IT Communication Standards}, series = {DSAI 2018 - 8th International Conference on Software Development and Technologies for Enhancing Accessibility and Fighting Info-exclusion}, booktitle = {DSAI 2018 - 8th International Conference on Software Development and Technologies for Enhancing Accessibility and Fighting Info-exclusion}, subject = {Mobile Application}, language = {en} } @misc{FrohnerMeyerDonsaetal., author = {Frohner, Matthias and Meyer, Markus and Donsa, Klaus and Urbauer, Philipp and David, Veronika and Sauermann, Stefan}, title = {Telemonitoring of Blood Glucose - A Prototype Android Application Enhancing the Patient / Health Professional Experience Using Health IT Communication Standards}, subject = {Mobile Application}, language = {en} } @inproceedings{SauermannDavidSchloegletal., author = {Sauermann, Stefan and David, Veronika and Schl{\"o}gl, Alois and Egelkraut, Reinhard and Frohner, Matthias and Pohn, Birgit and Urbauer, Philipp and Mense, Alexander}, title = {Biosignals, Standards and FHIR - The Way to Go?}, series = {Stud Health Technol Inform}, booktitle = {Stud Health Technol Inform}, pages = {356 -- 362}, subject = {Biosignals}, language = {en} } @misc{Sauermann, author = {Sauermann, Stefan}, title = {IHE / Compliance - Interoperabilit{\"a}t und Hygiene - gibt es Gemeinsamkeiten?}, subject = {Telemonitoring}, language = {de} } @misc{LitzenbergerChristensenHofstaetteretal., author = {Litzenberger, Stefan and Christensen, Troels and Hofst{\"a}tter, Otto and Sabo, Anton}, title = {Prediction of Road Surface Quality during Cycling Using Smartphone Accelerometer Data}, subject = {Cycling}, language = {en} } @misc{Sauermann, author = {Sauermann, Stefan}, title = {Flashlight: Telemonitoring Rahmenrichtlinie}, subject = {Telemonitoring}, language = {de} } @inproceedings{GastingerLitzenbergerSabo, author = {Gastinger, Roman and Litzenberger, Stefan and Sabo, Anton}, title = {Design, development and construction of a monitoring table tennis net}, series = {Procedia Engineering}, booktitle = {Procedia Engineering}, pages = {297 -- 303}, subject = {Sport}, language = {en} } @misc{SauermannFrohner, author = {Sauermann, Stefan and Frohner, Matthias}, title = {Wie vernetzen wir Medizintechnik und IT in OP und ICU}, subject = {Surgery}, language = {de} } @misc{Sauermann, author = {Sauermann, Stefan}, title = {Digital Healthcare als Werkzeug f{\"u}r die nahtlose Integrierte Versorgung}, subject = {Telemedicine}, language = {de} } @misc{Sauermann, author = {Sauermann, Stefan}, title = {Die digitale Revolution und ihre Auswirkungen auf die Gesellschaft}, subject = {Interoperability}, language = {de} } @misc{FrohnerForjan, author = {Frohner, Matthias and Forjan, Mathias}, title = {Interoperability in the OR Introducing OR.NET}, subject = {Interoperability}, language = {de} } @techreport{Pasteka, author = {Pasteka, Richard}, title = {Analysis of influences on inhalable aerosols under characteristic respiratory simulation conditions}, organization = {Brno University of Technology}, subject = {Mechanical Lung}, language = {en} } @inproceedings{DavidForjanPaštěkaetal., author = {David, Veronika and Forjan, Mathias and Paštěka, Richard and Scherer, Matthias and Hofst{\"a}tter, Otto}, title = {Development of a Multi-Purpose Easy-to-Use Set of Tools for Home Based Rehabilitation}, series = {DSAI 2018 - 8th International Conference on Software Development and Technologies for Enhancing Accessibility and Fighting Info-exclusion}, booktitle = {DSAI 2018 - 8th International Conference on Software Development and Technologies for Enhancing Accessibility and Fighting Info-exclusion}, subject = {Rehabitation}, language = {en} } @inproceedings{PastekaForjanDavid, author = {Pasteka, Richard and Forjan, Mathias and David, Veronika}, title = {A Single Point of Contact Data Platform for Rehabilitative Exercises and Equipment}, series = {DSAI 2018 - 8th International Conference on Software Development and Technologies for Enhancing Accessibility and Fighting Info-exclusion}, booktitle = {DSAI 2018 - 8th International Conference on Software Development and Technologies for Enhancing Accessibility and Fighting Info-exclusion}, subject = {Telemedicine}, language = {en} } @misc{SchererZapfSalomonetal., author = {Scherer, Matthias and Zapf, Stefan and Salomon, Cornelia and Kotzian, Stefan and Forjan, Mathias}, title = {Development of a virtual environment for the rehabilitation of neurological patients}, subject = {Virtual Supermarket}, language = {en} } @inproceedings{FrohnerGottschalkFranzletal., author = {Frohner, Matthias and Gottschalk, Marion and Franzl, Gerald and Pasteka, Richard and Uslar, Mathias and Sauermann, Stefan}, title = {Smart Grid Interoperability Profiles Development}, series = {IEEE International Conference on Smart Grid Communications}, booktitle = {IEEE International Conference on Smart Grid Communications}, subject = {Smart Grid}, language = {en} } @misc{TkachenkoBrilDavidForjanetal., author = {Tkachenko Bril, Andres Igor and David, Veronika and Forjan, Mathias and Gaudernak, Jakob and Pils, Katharina}, title = {Evaluation of the feasibility of a partial weight-bearing supporting biofeedback system based on instrumented insoles and an Android application}, subject = {Weight-Bearing}, language = {en} } @misc{DavidForjanPaštěkaetal., author = {David, Veronika and Forjan, Mathias and Paštěka, Richard and Scherer, Matthias and Hofst{\"a}tter, Otto}, title = {Development of a Multi-Purpose Easy-to-Use Set of Tools for Home Based Rehabilitation}, subject = {Rehabitation}, language = {en} } @misc{FrohnerForjan, author = {Frohner, Matthias and Forjan, Mathias}, title = {Interoperability Challenges - How to integrate medical devices}, subject = {Interoperability}, language = {en} } @misc{DavidForjan, author = {David, Veronika and Forjan, Mathias}, title = {Rehabilitation at Home - a Device supported Approach}, subject = {Rehabilitation}, language = {en} } @misc{MuehlederFuchsBassilioetal., author = {M{\"u}hleder, Severin and Fuchs, Christiane and Bassilio, Jose and Sczwarc, Dorota and Pill, Karoline and Slezak, Paul and Labuda, Krystina and Siehs, Christian and Pr{\"o}ll, Johannes and Priglinger, Eleni and Redl, Heinz and Holnthoner, Wolfgang}, title = {The purinergic receptor P2Y2 modulates endothelial sprouting and angiogenesis}, subject = {Angiogenesis}, language = {en} } @article{SchneiderEnayatiGrasletal., author = {Schneider, Karl Heinrich and Enayati, Marjan and Grasl, Christian and Walter, Ingrid and Budinsky, Lubos and Zebic, Gabriel and Kaun, Christoph and Wagner, Anja and Kratochwill, Klaus and Redl, Heinz and Teuschl, Andreas and Podesser, Bruno K. and Bergmeister, Helga}, title = {Acellular vascular matrix grafts from human placenta chorion: Impact of ECM preservation on graft characteristics, protein composition and in vivo performance.}, series = {Biomaterials}, journal = {Biomaterials}, pages = {14 -- 26}, abstract = {Small diameter vascular grafts from human placenta, decellularized with either Triton X-100 (Triton) or SDS and crosslinked with heparin were constructed and characterized. Graft biochemical properties, residual DNA, and protein composition were evaluated to compare the effect of the two detergents on graft matrix composition and structural alterations. Biocompatibility was tested in vitro by culturing the grafts with primary human macrophages and in vivo by subcutaneous implantation of graft conduits (n = 7 per group) into the flanks of nude rats. Subsequently, graft performance was evaluated using an aortic implantation model in Sprague Dawley rats (one month, n = 14). In situ graft imaging was performed using MRI angiography. Retrieved specimens were analyzed by electromyography, scanning electron microscopy, histology and immunohistochemistry to evaluate cell migration and the degree of functional tissue remodeling. Both decellularization methods resulted in grafts of excellent biocompatibility in vitro and in vivo, with low immunogenic potential. Proteomic data revealed removal of cytoplasmic proteins with relative enrichment of ECM proteins in decelluarized specimens of both groups. Noteworthy, LC-Mass Spectrometry analysis revealed that 16 proteins were exclusively preserved in Triton decellularized specimens in comparison to SDS-treated specimens. Aortic grafts showed high patency rates, no signs of thrombus formation, aneurysms or rupture. Conduits of both groups revealed tissue-specific cell migration indicative of functional remodeling. This study strongly suggests that decellularized allogenic grafts from the human placenta have the potential to be used as vascular replacement materials. Both detergents produced grafts with low residual immunogenicity and appropriate mechanical properties. Observed differences in graft characteristics due to preservation method had no impact on successful in vivo performance in the rodent model.}, subject = {Biomaterial}, language = {en} } @article{PriglingerSchuhSteffenhagenetal., author = {Priglinger, Eleni and Schuh, Christina and Steffenhagen, Carolin and Wurzer, Christoph and Maier, Julia and N{\"u}rnberger, Sylvia and Holnthoner, Wolfgang and Fuchs, Christiane and Suessner, Susanne and R{\"u}nzler, Dominik and Redl, Heinz and Wolbank, Susanne}, title = {Improvement of adipose tissue-derived cells by low-energy extracorporeal shock wave therapy.}, series = {Cytotherapy}, journal = {Cytotherapy}, pages = {1079 -- 1095}, abstract = {BACKGROUND: Cell-based therapies with autologous adipose tissue-derived cells have shown great potential in several clinical studies in the last decades. The majority of these studies have been using the stromal vascular fraction (SVF), a heterogeneous mixture of fibroblasts, lymphocytes, monocytes/macrophages, endothelial cells, endothelial progenitor cells, pericytes and adipose-derived stromal/stem cells (ASC) among others. Although possible clinical applications of autologous adipose tissue-derived cells are manifold, they are limited by insufficient uniformity in cell identity and regenerative potency. METHODS: In our experimental set-up, low-energy extracorporeal shock wave therapy (ESWT) was performed on freshly obtained human adipose tissue and isolated adipose tissue SVF cells aiming to equalize and enhance stem cell properties and functionality. RESULTS: After ESWT on adipose tissue we could achieve higher cellular adenosine triphosphate (ATP) levels compared with ESWT on the isolated SVF as well as the control. ESWT on adipose tissue resulted in a significantly higher expression of single mesenchymal and vascular marker compared with untreated control. Analysis of SVF protein secretome revealed a significant enhancement in insulin-like growth factor (IGF)-1 and placental growth factor (PLGF) after ESWT on adipose tissue. DISCUSSION: Summarizing we could show that ESWT on adipose tissue enhanced the cellular ATP content and modified the expression of single mesenchymal and vascular marker, and thus potentially provides a more regenerative cell population. Because the effectiveness of autologous cell therapy is dependent on the therapeutic potency of the patient's cells, this technology might raise the number of patients eligible for autologous cell transplantation.}, subject = {Shockwave Therapy}, language = {en} }