TY  - JOUR
A1  - Jesenberger, Veronika
A1  - Zmajkovicova, Katarina
A1  - Catalanotti, Federica
A1  - Baumgartner, Christian
A1  - Reyes, Gloria Ximena
A1  - Baccarini, Manuela
T1  - MEK1 is required for PTEN membrane recruitment, AKT regulation, and the maintenance of peripheral tolerance
JF  - Mol. Cell
N2  - The Raf/MEK/ERK and PI3K/Akt pathways are prominent effectors of oncogenic Ras. These pathways negatively regulate each other, but the mechanism involved is incompletely understood. We now identify MEK1 as an essential regulator of lipid/protein phosphatase PTEN, through which it controls phosphatidylinositol-3-phosphate accumulation and AKT signaling. MEK1 ablation stabilizes AKT activation and, in vivo, causes a lupus-like autoimmune disease and myeloproliferation. Mechanistically, MEK1 is necessary for PTEN membrane recruitment as part of a ternary complex containing the multidomain adaptor MAGI1. Complex formation is independent of MEK1 kinase activity but requires phosphorylation of T292 on MEK1 by activated ERK. Thus, inhibiting the ERK pathway reduces PTEN membrane recruitment, increasing phosphatidylinositol-3-phosphate accumulation and AKT activation. Our data offer a conceptual framework for the observation that activation of the PI3K pathway frequently mediate resistance to MEK inhibitors and for the promising results obtained by combined MEK/PI3K inhibition in preclinical cancer models.
KW  - MEK1 pathway
KW  - membrane recruitment
KW  - preclinical cancer models
Y1  - 2020
VL  - 2013
IS  - 50
SP  - 43
EP  - 55
ER  - 
TY  - JOUR
A1  - Catalanotti, Federica
A1  - Reyes, Gloria Ximena
A1  - Jesenberger, Veronika
A1  - Galabova-Kovacs, Gergana
A1  - de Matos Simoes, Ricardo
A1  - Carugo, Oliviero
A1  - Baccarini, Manuela
T1  - A Mek1-Mek2 heterodimer determines the strength and duration of the Erk signal
JF  - Nat Struct Mol Biol.
N2  - Mek1 and Mek2 (also known as Map2k1 and Map2k2, respectively) are evolutionarily conserved, dual-specificity kinases that mediate Erk1 and Erk2 activation during adhesion and growth factor signaling. Here we describe a previously uncharacterized, unexpected role of Mek1 in downregulating Mek2-dependent Erk signaling. Mek1 mediates the regulation of Mek2 in the context of a previously undiscovered Mek1-Mek2 complex. The Mek heterodimer is negatively regulated by Erk-mediated phosphorylation of Mek1 on Thr292, a residue missing in Mek2. Disabling this Erk-proximal negative-feedback step stabilizes the phosphorylation of both Mek2 and Erk in cultured cells and in vivo in Mek1 knockout embryos and mice. Thus, in disagreement with the current perception of the pathway, the role of Mek1 and Mek2 in growth factor-induced Erk phosphorylation is not interchangeable. Our data establish Mek1 as the crucial modulator of Mek and Erk signaling and have potential implications for the role of Mek1 and Mek2 in tumorigenesis.
KW  - Mek1-Mek2 pathway
KW  - tumorigenesis
KW  - ERK activation
KW  - cancer biology
Y1  - 2020
VL  - 2009
IS  - 16(3)
SP  - 294
EP  - 303
ER  -