TY  - JOUR
A1  - Deininger, Christian
A1  - Wagner, Andrea
A1  - Heimel, Patrick
A1  - Salzer, Elias
A1  - Monforte Vila, Xavier
A1  - Weißenbacher, Nadja
A1  - Grillari, Johannes
A1  - Redl, Heinz
A1  - Wichlas, Florian
A1  - Freude, Thomas
A1  - Tempfer, Herbert
A1  - Teuschl-Woller, Andreas
A1  - Traweger, Andreas
T1  - Enhanced BMP-2-Mediated Bone Repair Using an Anisotropic Silk Fibroin Scaffold Coated with Bone-like Apatite
JF  - Int. J. Mol. Sci.
N2  - The repair of large bone defects remains challenging and often requires graft material due to limited availability of autologous bone. In clinical settings, collagen sponges loaded with excessive amounts of bone morphogenetic protein 2 (rhBMP-2) are occasionally used for the treatment of bone non-unions, increasing the risk of adverse events. Therefore, strategies to reduce rhBMP-2 dosage are desirable. Silk scaffolds show great promise due to their favorable biocompatibility and their utility for various biofabrication methods. For this study, we generated silk scaffolds with axially aligned pores, which were subsequently treated with 10× simulated body fluid (SBF) to generate an apatitic calcium phosphate coating. Using a rat femoral critical sized defect model (CSD) we evaluated if the resulting scaffold allows the reduction of BMP-2 dosage to promote efficient bone repair by providing appropriate guidance cues. Highly porous, anisotropic silk scaffolds were produced, demonstrating good cytocompatibility in vitro and treatment with 10× SBF resulted in efficient surface coating. In vivo, the coated silk scaffolds loaded with a low dose of rhBMP-2 demonstrated significantly improved bone regeneration when compared to the unmineralized scaffold. Overall, our findings show that this simple and cost-efficient technique yields scaffolds that enhance rhBMP-2 mediated bone healing.
KW  - Tissue Engineering
KW  - Biomaterials
KW  - silk scaffold
KW  - bone regeneration
KW  - pseudoarthrosis
Y1  - 
VL  - 23
IS  - 1 / 283
ER  - 
TY  - JOUR
A1  - Bernhard, Jonathan
A1  - Ferguson, James
A1  - Rieder, Bernhard
A1  - Heimel, Patrick
A1  - Nau, Thomas
A1  - Tangl, Stefan
A1  - Redl, Heinz
A1  - Vunjak-Novakovic, Gordana
T1  - Tissue-engineered hypertrophic chondrocyte grafts enhanced long bone repair biomaterials
JF  - Biomaterials
KW  - Grafting
KW  - Biomaterials
Y1  - 2018
IS  - 139
SP  - 202
EP  - 212
ER  -