TY  - JOUR
A1  - Tomasch, Janine
A1  - Maleiner, Babette
A1  - Hromada, Carina
A1  - Szwarc-Hofbauer, Dorota
A1  - Teuschl-Woller, Andreas
T1  - Cyclic Tensile Stress Induces Skeletal Muscle Hypertrophy and Myonuclear Accretion in a 3D Model
JF  - Tissue Eng. Part A.
N2  - Skeletal muscle is highly adaptive to mechanical stress due to its resident stem cells and the pronounced level of myotube plasticity. Herein, we study the adaptation to mechanical stress and its underlying molecular mechanisms in a tissue-engineered skeletal muscle model. We subjected differentiated 3D skeletal muscle-like constructs to cyclic tensile stress using a custom-made bioreactor system, which resulted in immediate activation of stress-related signal transducers (Erk1/2, p38). Cell cycle re-entry, increased proliferation, and onset of myogenesis indicated subsequent myoblast activation. Furthermore, elevated focal adhesion kinase and β-catenin activity in mechanically stressed constructs suggested increased cell adhesion and migration. After 3 days of mechanical stress, gene expression of the fusogenic markers MyoMaker and MyoMixer, myotube diameter, myonuclear accretion, as well as S6 activation, were significantly increased. Our results highlight that we established a promising tool to study sustained adaptation to mechanical stress in healthy, hypertrophic, or regenerating skeletal muscle.
KW  - fibrin
KW  - tissue engineering
KW  - tensile stress
KW  - regeneration
KW  - hypertrophy
Y1  - 
VL  - 2023
IS  - Mar
SP  - 257
EP  - 268
ER  - 
TY  - GEN
A1  - Hromada, Carina
A1  - Tomasch, Janine
A1  - Weihs, Anna
A1  - Rünzler, Dominik
A1  - Teuschl, Andreas
T1  - Engineering of 3D Tissue Constructs Using our Novel MagneTissue Bioreactor as Alternatives to Animal Models
KW  - Bioreactor
KW  - Biomaterials
Y1  - 
ER  - 
TY  - JOUR
A1  - Hromada, Carina
A1  - Hartmann, Jaana
A1  - Oesterreicher, Johannes
A1  - Stoiber, Anton
A1  - Daerr, Anna
A1  - Schädl, Barbara
A1  - Priglinger, Eleni
A1  - Teuschl-Woller, Andreas H.
A1  - Holnthoner, Wolfgang
A1  - Heinzel, Johannes Christoph
A1  - Hercher, David
T1  - Occurrence of Lymphangiogenesis in Peripheral Nerve Autografts Contrasts Schwann Cell-Induced Apoptosis of Lymphatic Endothelial Cells In Vitro
JF  - Biomolecules
N2  - Peripheral nerve injuries pose a major clinical concern world-wide, and functional recovery after segmental peripheral nerve injury is often unsatisfactory, even in cases of autografting. Although it is well established that angiogenesis plays a pivotal role during nerve regeneration, the influence of lymphangiogenesis is strongly under-investigated. In this study, we analyzed the presence of lymphatic vasculature in healthy and regenerated murine peripheral nerves, revealing that nerve autografts contained increased numbers of lymphatic vessels after segmental damage. This led us to elucidate the interaction between lymphatic endothelial cells (LECs) and Schwann cells (SCs) in vitro. We show that SC and LEC secretomes did not influence the respective other cell types' migration and proliferation in 2D scratch assay experiments. Furthermore, we successfully created lymphatic microvascular structures in SC-embedded 3D fibrin hydrogels, in the presence of supporting cells; whereas SCs seemed to exert anti-lymphangiogenic effects when cultured with LECs alone. Here, we describe, for the first time, increased lymphangiogenesis after peripheral nerve injury and repair. Furthermore, our findings indicate a potential lymph-repellent property of SCs, thereby providing a possible explanation for the lack of lymphatic vessels in the healthy endoneurium. Our results highlight the importance of elucidating the molecular mechanisms of SC-LEC interaction.
KW  - Tissue Engineering
KW  - peripheral nerve regeneration
KW  - lymphangiogenesis
KW  - Schwann cells
KW  - lymphatic endothelial cells
Y1  - 
VL  - 2022
IS  - 12, 6
SP  - 820
ER  -