TY  - JOUR
A1  - Slezak, Paul
A1  - Slezak, Cyrill
A1  - Hartinger, Joachim
A1  - Teuschl, Andreas
A1  - Nürnberger, Sylvia
A1  - Redl, Heinz
A1  - Mittermayr, Rainer
T1  - A Low Cost Implantation Model in the Rat That Allows a Spatial Assessment of Angiogenesis.
JF  - Frontiers in Bioengineering and Biotechnology
N2  - There is continual demand for animal models that allow a quantitative assessment of angiogenic properties of biomaterials, therapies, and pharmaceuticals. In its simplest form, this is done by subcutaneous material implantation and subsequent vessel counting which usually omits spatial data. We have refined an implantation model and paired it with a computational analytic routine which outputs not only vessel count but also vessel density, distribution, and vessel penetration depth, that relies on a centric vessel as a reference point. We have successfully validated our model by characterizing the angiogenic potential of a fibrin matrix in conjunction with recombinant human vascular endothelial growth factor (rhVEGF165). The inferior epigastric vascular pedicles of rats were sheathed with silicone tubes, which were subsequently filled with 0.2 ml of fibrin and different doses of rhVEGF165, centrically embedding the vessels. Over 4 weeks, tissue samples were harvested and subsequently immunohistologically stained and computationally analyzed. The model was able to detect variations over the angiogenic potentials of growth factor spiked fibrin matrices. Adding 20 ng of rhVEGF165 resulted in a significant increase in vasculature while 200 ng of rhVEGF165 did not improve vascular growth. Vascularized tissue volume increased during the first week and vascular density increased during the second week. Total vessel count increased significantly and exhibited a peak after 2 weeks which was followed by a resorption of vasculature by week 4. In summary, a simple implantation model to study in vivo vascularization with only a minimal workload attached was enhanced to include morphologic data of the emerging vascular tree.
KW  - Tissue Engineering
KW  - Bioreactor
KW  - Biomaterial
Y1  - 
ER  - 
TY  - GEN
A1  - Slezak, Paul
A1  - Rose, Roland
A1  - Hercher, David
A1  - Weihs, Anna
A1  - Fuchs, Christiane
A1  - Redl, Heinz
A1  - Mittermayr, Rainer
A1  - Slezak, Cyrill
T1  - Tracking therapeutic shockwaves and their impact on regeneration
KW  - Shockwave Therapy
KW  - Tissue Regeneration
Y1  - 
ER  -