TY  - JOUR
A1  - Schneider, Jaana
A1  - Pultar, Marianne
A1  - Oesterreicher, Johannes
A1  - Bobbili, Madhusudhan Reddy
A1  - Mühleder, Severin
A1  - Priglinger, Eleni
A1  - Redl, Heinz
A1  - Spittler, Andreas
A1  - Grillari, Johannes
A1  - Holnthoner, Wolfgang
T1  - Cre mRNA Is Not Transferred by EVs from Endothelial and Adipose-Derived Stromal/Stem Cells during Vascular Network Formation
JF  - Int J Mol Sci.
N2  - Coculture systems employing adipose tissue-derived mesenchymal stromal/stem cells (ASC) and endothelial cells (EC) represent a widely used technique to model vascularization. Within this system, cell-cell communication is crucial for the achievement of functional vascular network formation. Extracellular vesicles (EVs) have recently emerged as key players in cell communication by transferring bioactive molecules between cells. In this study we aimed to address the role of EVs in ASC/EC cocultures by discriminating between cells, which have received functional EV cargo from cells that have not. Therefore, we employed the Cre-loxP system, which is based on donor cells expressing the Cre recombinase, whose mRNA was previously shown to be packaged into EVs and reporter cells containing a construct of floxed dsRed upstream of the eGFP coding sequence. The evaluation of Cre induced color switch in the reporter system via EVs indicated that there is no EV-mediated RNA transmission either between EC themselves or EC and ASC. However, since Cre mRNA was not found present in EVs, it remains unclear if Cre mRNA is generally not packaged into EVs or if EVs are not taken up by the utilized cell types. Our data indicate that this technique may not be applicable to evaluate EV-mediated cell-to-cell communication in an in vitro setting using EC and ASC. Further investigations will require a functional system showing efficient and specific loading of Cre mRNA or protein into EVs.
KW  - Tissue Engineering
KW  - Stem Cells
KW  - Vascular Network Formation
KW  - EVs
KW  - Endothelial Cells
Y1  - 
VL  - 2021
IS  - 22(8)
SP  - 4050
ER  - 
TY  - JOUR
A1  - Trisko, Johanna
A1  - Fleck, Johanna
A1  - Kau, Silvio
A1  - Oesterreicher, Johannes
A1  - Holnthoner, Wolfgang
T1  - Lymphatic and Blood Endothelial Extracellular Vesicles: A Story Yet to Be Written
JF  - Life
N2  - Extracellular vesicles (EVs), such as exosomes, microvesicles, and apoptotic bodies, are cell-derived, lipid bilayer-enclosed particles mediating intercellular communication and are therefore vital for transmitting a plethora of biological signals. The vascular endothelium substantially contributes to the circulating particulate secretome, targeting important signaling pathways that affect blood cells and regulate adaptation and plasticity of endothelial cells in a paracrine manner. Different molecular signatures and functional properties of endothelial cells reflect their heterogeneity among different vascular beds and drive current research to understand varying physiological and pathological effects of blood and lymphatic endothelial EVs. Endothelial EVs have been linked to the development and progression of various vascular diseases, thus having the potential to serve as biomarkers and clinical treatment targets. This review aims to provide a brief overview of the human vasculature, the biology of extracellular vesicles, and the current knowledge of endothelium-derived EVs, including their potential role as biomarkers in disease development.
KW  - Tissue Engineering
KW  - Endothelial Cells
KW  - vascularization
KW  - extracellular vesicles
KW  - lymphatic endothelial cells
Y1  - 
VL  - 2022
IS  - 12(5)
SP  - 654
ER  -