TY  - JOUR
A1  - Purtscher, Michaela
A1  - Rothbauer, Mario
A1  - Kratz, Sebastian Rudi Adam
A1  - Bailey, Andrew
A1  - Lieberzeit, Peter
A1  - Ertl, Peter
T1  - A microfluidic impedance-based extended infectivity assay: combining retroviral amplification and cytopathic effect monitoring on a single lab-on-a-chip platform
JF  - Lab on a Chip
N2  - Detection, quantification and monitoring of virus – host cell interactions are of great importance when evaluating the safety of pharmaceutical products. With the wide usage of viral based vector systems in combination with mammalian cell lines for the production of biopharmaceuticals, the presence of replication competent viral particles needs to be avoided and potential hazards carefully assessed. Consequently, regulatory agencies recommend viral clearance studies using plaque assays or TCID50 assays to evaluate the efficiency of the production process in removing viruses. While plaque assays provide reliable information on the presence of viral contaminations, they are still tedious to perform and can take up to two weeks to finish. To overcome some of these limitations, we have automated, miniaturized and integrated the dual cell culture bioassay into a common lab-on-a-chip platform containing embedded electrical sensor arrays to enrich and detect infectious viruses. Results of our microfluidic single step assay show that a significant reduction in assay time down to 3 to 4 days can be achieved using simultaneous cell-based viral amplification, release and detection of cytopathic effects in a target cell line. We further demonstrate the enhancing effect of continuous fluid flow on infection of PG-4 reporter cells by newly formed and highly active virions by M. dunni cells, thus pointing to the importance of physical relevant viral–cell interactions.
KW  - Tissue Engineering
KW  - Microfluidics
KW  - Lab on Chip
Y1  - 2021
VL  - 2021
IS  - Issue 7
SP  - 1364
EP  - 1372
ER  - 
TY  - GEN
A1  - Szwarc, Dorota
A1  - Fuchs, Christiane
A1  - Purtscher, Michaela
A1  - Rünzler, Dominik
T1  - Elucidating the molecular mechanisms underlying cardiac shock wave therapy
KW  - Shockwave treatment
KW  - Cardiac
Y1  - 2018
ER  - 
TY  - JOUR
A1  - Rosser, Julie
A1  - Calvo, Isabel Olmos
A1  - Schlager, Magdalena
A1  - Purtscher, Michaela
A1  - Jenner, Florien
A1  - Ertl, Peter
T1  - Recent Advances of Biologically Inspired 3D Microfluidic Hydrogel Cell Culture Systems
JF  - J Cell Biol Cell Metalab
KW  - Hydrogel
KW  - Cell Culture
Y1  - 
IS  - 2
ER  - 
TY  - GEN
A1  - Stoiber, Stefan
A1  - Purtscher, Michaela
A1  - Gepp, Barbara
A1  - Huber-Gries, Carina
T1  - Towards a platform for spatially defined cell characterization of a miniturized heart tissue model
KW  - Microfluidic
KW  - Tissue Engineering
KW  - Heart
Y1  - 
ER  - 
TY  - GEN
A1  - Purtscher, Michaela
A1  - Ergir, Ece
A1  - Szwarc, Dorota
A1  - Monforte Vila, Xavier
A1  - Rünzler, Dominik
A1  - Huber-Gries, Carina
T1  - A microfluidic-based easy-to-use cardiac tissue model for drug screening applications
KW  - Tissue Generation
KW  - Drug Screening
KW  - Cardiac
Y1  - 2018
ER  - 
TY  - GEN
A1  - Purtscher, Michaela
A1  - Ergir, Ece
A1  - Monforte Vila, Xavier
A1  - Huber-Gries, Carina
T1  - Establishment of an in vitro heart tissue model for pre-clinical screening of therapeutic agents using microfluidic technology
KW  - Tissue Generation
KW  - Heart Tissue
Y1  - 2018
ER  - 
TY  - GEN
A1  - Purtscher, Michaela
A1  - Ergir, Ece
A1  - Szwarc, Dorota
A1  - Huber-Gries, Carina
T1  - Microfluidic based heart-tissue model for directed development of cardiac specific cell types
KW  - Heart Tissue
KW  - Tissue Generation
KW  - Cardiac
Y1  - 2018
ER  - 
TY  - JOUR
A1  - Bachmann, Barbara
A1  - Spitz, Sarah
A1  - Rothbauer, Mario
A1  - Jordan, Christian
A1  - Purtscher, Michaela
A1  - Zirath, Helene
A1  - Schuller, Patrick
A1  - Eilenberger, Christoph
A1  - Ali, Syed Faheem
A1  - Mühleder, Severin
A1  - Priglinger, Eleni
A1  - Harasek, Michael
A1  - Redl, Heinz
A1  - Holnthoner, Wolfgang
A1  - Ertl, Peter
T1  - Engineering of three-dimensional pre-vascular networks within fibrin hydrogel constructs by microfluidic control over reciprocal cell signaling
JF  - Biomicrofluidics
KW  - Microfluidic
KW  - Vascularization
KW  - Tissue Engineering
Y1  - 2019
ER  -