TY  - JOUR
A1  - Maleiner, Babette
A1  - Tomasch, Janine
A1  - Heher, Philipp
A1  - Spadiut, Oliver
A1  - Rünzler, Dominik
A1  - Fuchs, Christiane
T1  - The Importance of Biophysical and Biochemical Stimuli in Dynamic Skeletal Muscle Models.
JF  - Frontiers in Physiology
N2  - Classical approaches to engineer skeletal muscle tissue based on current regenerative and surgical procedures still do not meet the desired outcome for patient applications. Besides the evident need to create functional skeletal muscle tissue for the repair of volumetric muscle defects, there is also growing demand for platforms to study muscle-related diseases, such as muscular dystrophies or sarcopenia. Currently, numerous studies exist that have employed a variety of biomaterials, cell types and strategies for maturation of skeletal muscle tissue in 2D and 3D environments. However, researchers are just at the beginning of understanding the impact of different culture settings and their biochemical (growth factors and chemical changes) and biophysical cues (mechanical properties) on myogenesis. With this review we intend to emphasize the need for new in vitro skeletal muscle (disease) models to better recapitulate important structural and functional aspects of muscle development. We highlight the importance of choosing appropriate system components, e.g., cell and biomaterial type, structural and mechanical matrix properties or culture format, and how understanding their interplay will enable researchers to create optimized platforms to investigate myogenesis in healthy and diseased tissue. Thus, we aim to deliver guidelines for experimental designs to allow estimation of the potential influence of the selected skeletal muscle tissue engineering setup on the myogenic outcome prior to their implementation. Moreover, we offer a workflow to facilitate identifying and selecting different analytical tools to demonstrate the successful creation of functional skeletal muscle tissue. Ultimately, a refinement of existing strategies will lead to further progression in understanding important aspects of muscle diseases, muscle aging and muscle regeneration to improve quality of life of patients and enable the establishment of new treatment options.
KW  - Bioreactor
KW  - Muscle
KW  - Biomaterial
Y1  - 
ER  - 
TY  - JOUR
A1  - Schuh, Christina
A1  - Heher, Philipp
A1  - Weihs, Anna
A1  - Fuchs, Christiane
A1  - Gabriel, Christian
A1  - Wolbank, Susanne
A1  - Mittermayr, Rainer
A1  - Redl, Heinz
A1  - Rünzler, Dominik
A1  - Teuschl, Andreas
T1  - In vitro extracorporeal shock wave treatment enhances stemness and preserves multipotency of rat and human adipose-derived stem cells
JF  - Journal of Cytotherapy
KW  - Shockwave
Y1  - 
ER  - 
TY  - CHAP
A1  - Köhler, Gottfried
A1  - Rünzler, Dominik
A1  - Mayer, B.
A1  - Sára, Margit
A1  - Rasmussen, S.
A1  - Buravkova, L.
T1  - Microgravity. Applications in Biotechnology and Bioorganic Materials Sciences
T2  - 10 Years Space biomedical Research and Development in Austria
KW  - Microgravity
KW  - Biotechnology
KW  - Materials Sciences
Y1  - 2019
SP  - 65
EP  - 85
PB  - Facultas
CY  - Wien
ER  - 
TY  - CHAP
A1  - Wießner, Katharina
A1  - Praher, Daniela
A1  - Liousia, Varvara
A1  - Gepp, Barbara
A1  - Leitner, Rita
A1  - Rünzler, Dominik
T1  - Testing the acute toxicity of an alternative to Glyphosate - Pelargonic acid
T2  - SETAC Europe 30th Annual Meeting - Online
N2  - Glyphosate-based herbicides were used as plant protection product globally for several decades. However, glyphosate is discussed as showing genotoxicity and many other side-effects such as inhibiting the mitochondrial succinate dehydrogenase, leading to a decreased ATP production. Therefore, finding alternative active substances is necessary. Pelargonic acid (nonanoic acid) and its ammonium salt (saponified form), which are used as alternatives to glyphosate-based herbicides, are biological derived substances considered as environmentally friendly herbicides. To test the effects of pelargonic acid in its acidic form (active substance in TopGun) and its saponified form (in Finalsan Plus) on the aquatic ecosystem, students of the master study program “environmental management and ecotoxicology” compared the toxicity levels of these substances using zebrafish embryos within the scope of a student research course. The project was developed by applying the 7-step problem-based learning method which allowed the students to design their experiments independently with the guidance and feedback of the lecturers. Acute toxicity was determined according to OECD test guideline 236 in D. rerio which revealed a LC50 of 1.55 mg/L of pelargonic acid, a LC50 value of 0.93 mg/L pelargonic acid in TopGun and a LC50 of 36.37 mg/L of Finalsan Plus. Neutral Red Uptake assays were performed on the rainbow trout-derived gill cell-line RTgill-W1 to determine the acute toxicity according to the OECD test guideline 129 which revealed the IC50 value of 12.4 mg/L pelargonic acid in TG. Due to solubility limits, no reliable IC50 could be obtained. The results of the tests indicate differences in the acute toxicity of the pelargonic acid-based formulations TopGun and Finalsan Plus. However, more investigations have to be done in order to analyse if the acidic form is more toxic than the saponified form. Furthermore, detailed ecotoxicological risk assessments and an evaluation of the genotoxicity of both herbicides has to be performed before any conclusion can be drawn. Financial support from the City of Vienna project PBL in Molecular Life Science (21-06) is gratefully acknowledged.
KW  - Environmental Chemistry
KW  - Ecotoxicology
Y1  - 2020
ER  - 
TY  - CHAP
A1  - Leitner, Rita
A1  - Gepp, Barbara
A1  - Liousia, Varvara
A1  - Rünzler, Dominik
T1  - Teaching 3R principles in ecotoxicology by performing a problem-based learning project
T2  - ALTEX Proceedings, LINZ 2018 – EUSAAT 2018, Volume 7, No. 2, ISSN 2194-0479 (2018)
KW  - Problem Based Learning
KW  - Teaching
KW  - Ecotoxicology
KW  - 3R Principles
Y1  - 
ER  - 
TY  - GEN
A1  - Gepp, Barbara
A1  - Liousia, Varvara
A1  - Machacek, Thomas
A1  - Praher, Daniela
A1  - Eisner, Katharina
A1  - Limbeck, Sophie
A1  - Gamper, Janine
A1  - Schwarzl, Elisabeth
A1  - Hazod, Till
A1  - Landwehr, Renate
A1  - Wenger, Charlotte
A1  - Olscher, Christoph
A1  - Leitner, Rita
A1  - Rünzler, Dominik
T1  - Pelargonic acid - an alternative to glyphosate-based herbicides?
KW  - Ecotoxicology
KW  - Herbicides
Y1  - 
ER  - 
TY  - GEN
A1  - Teuschl, Andreas
A1  - Schuh, Christina
A1  - Weihs, Anna
A1  - Guillaume, Olivier
A1  - Monforte Vila, Xavier
A1  - Redl, Heinz
A1  - Kaplan, David
A1  - Rünzler, Dominik
T1  - Tailoring bioactivity of silk-based biomaterials via delivering and functionalization strategies with fibrinogen/thrombin, plant lectins or laminin
KW  - Biomaterials
KW  - Tissue Engineering
KW  - Silk
Y1  - 
ER  - 
TY  - GEN
A1  - Salzer, Elias
A1  - Rieder, Bernhard
A1  - Monforte Vila, Xavier
A1  - Weihs, Anna
A1  - Rünzler, Dominik
A1  - Teuschl, Andreas
T1  - Evaluation of a novel hydrostatic pressure bioreactor on bovine cartilage chips
KW  - Bioreactor
KW  - Tissue Engineering
KW  - Cartilage
Y1  - 
ER  - 
TY  - GEN
A1  - Eisner, Katharina
A1  - Gepp, Barbara
A1  - Schnegg, Raimund
A1  - Dallinger, Reinhard
A1  - Rünzler, Dominik
T1  - Investigation of endocrine effects of sublethal cadmium doses on the reproduction system of the freshwater snail B. glabrata
KW  - Ecotoxicology
KW  - Endocrine disruptors
Y1  - 
ER  - 
TY  - GEN
A1  - Salzer, Elias
A1  - Liousia, Varvara
A1  - Monforte Vila, Xavier
A1  - Rünzler, Dominik
T1  - Tracking of small aquatic organisms with custom-made tracking plates
KW  - Ecotoxicology
KW  - Behavior Analysis
KW  - Aquatic Ecotoxicology
Y1  - 
ER  - 
TY  - CHAP
A1  - Fuchs, Christiane
A1  - Weihs, Anna
A1  - Szwarc, Dorota
A1  - Mittermayr, Rainer
A1  - Rünzler, Dominik
A1  - Teuschl, Andreas
T1  - Shock wave treatment of muscle (stem) cells - a new implementation for regeneration
T2  - Proceedings of the 20th International Congress of the ISMST
KW  - Shockwave treatment
KW  - Muscle Cells
KW  - Regeneration
Y1  - 2018
ER  - 
TY  - GEN
A1  - Fuchs, Christiane
A1  - Weihs, Anna
A1  - Szwarc, Dorota
A1  - Mittermayr, Rainer
A1  - Rünzler, Dominik
A1  - Teuschl, Andreas
T1  - Shock wave treatment of muscle (stem) cells - a new implementation for regeneration
KW  - Shockwave treatment
KW  - Muscle Cells
KW  - Regeneration
Y1  - 2018
ER  - 
TY  - GEN
A1  - Szwarc, Dorota
A1  - Fuchs, Christiane
A1  - Weihs, Anna
A1  - Rünzler, Dominik
T1  - Molecular mechanisms underlying the potential of shock wave treatment for cardiac therapy
KW  - Shockwave treatment
KW  - Cardiac
Y1  - 2018
ER  - 
TY  - GEN
A1  - Szwarc, Dorota
A1  - Fuchs, Christiane
A1  - Purtscher, Michaela
A1  - Rünzler, Dominik
T1  - Elucidating the molecular mechanisms underlying cardiac shock wave therapy
KW  - Shockwave treatment
KW  - Cardiac
Y1  - 2018
ER  - 
TY  - GEN
A1  - Rünzler, Dominik
T1  - Alternatives to Animal Experiment Models: Tracking Zebrafish Larvae and Daphnia
KW  - Animal Experiments
KW  - Zebrafish
Y1  - 2018
ER  - 
TY  - CHAP
A1  - Maleiner, Babette
A1  - Heher, Philipp
A1  - Teuschl, Andreas
A1  - Redl, Heinz
A1  - Rünzler, Dominik
A1  - Fuchs, Christiane
T1  - Generation of aligned skeletal muscle-like tissue based on the application of strain to a 3D fibrin scaffold
T2  - Proceedings of PACT "Designer Cells go Clinical" Symposium
KW  - Tissue Generation
KW  - Fibrin
KW  - Scaffold
Y1  - 2018
ER  - 
TY  - GEN
A1  - Szwarc, Dorota
A1  - Fuchs, Christiane
A1  - Weihs, Anna
A1  - Monforte Vila, Xavier
A1  - Hanetseder, Dominik
A1  - Teuschl, Andreas
A1  - Rünzler, Dominik
T1  - The effect of shock waves on in vitro cartilage development in silk scaffolds
KW  - Shockwave treatment
KW  - In Vitro
KW  - Cartilage
KW  - Silk
Y1  - 2018
ER  - 
TY  - GEN
A1  - Maleiner, Babette
A1  - Heher, Philipp
A1  - Teuschl, Andreas
A1  - Redl, Heinz
A1  - Rünzler, Dominik
A1  - Fuchs, Christiane
T1  - Generation of aligned skeletal muscle-like tissue based on the application of strain to a 3D fibrin scaffold
KW  - Tissue Generation
KW  - Fibrin
KW  - Scaffold
Y1  - 2018
ER  - 
TY  - GEN
A1  - Teuschl, Andreas
A1  - Fuchs, Christiane
A1  - Weihs, Anna
A1  - Heimel, Patrick
A1  - Rünzler, Dominik
A1  - Redl, Heinz
A1  - Nau, Thomas
T1  - The Silk Road from Textiles to Novel Medical Implants
KW  - Silk
Y1  - 2018
ER  - 
TY  - CHAP
A1  - Fuchs, Christiane
A1  - Szwarc, Dorota
A1  - Weihs, Anna
A1  - Rünzler, Dominik
T1  - Shock wave treatment of 3D cardiac model systems activates ERK 1/2 signaling pathway and influences cardiomyogenesis
T2  - Proceedings of PACT "Designer Cells go Clinical" Symposium
KW  - Shockwave treatment
KW  - Cardiac
Y1  - 2018
ER  -