TY - CHAP A1 - Wießner, Katharina A1 - Praher, Daniela A1 - Liousia, Varvara A1 - Gepp, Barbara A1 - Leitner, Rita A1 - Rünzler, Dominik T1 - Testing the acute toxicity of an alternative to Glyphosate - Pelargonic acid T2 - SETAC Europe 30th Annual Meeting - Online N2 - Glyphosate-based herbicides were used as plant protection product globally for several decades. However, glyphosate is discussed as showing genotoxicity and many other side-effects such as inhibiting the mitochondrial succinate dehydrogenase, leading to a decreased ATP production. Therefore, finding alternative active substances is necessary. Pelargonic acid (nonanoic acid) and its ammonium salt (saponified form), which are used as alternatives to glyphosate-based herbicides, are biological derived substances considered as environmentally friendly herbicides. To test the effects of pelargonic acid in its acidic form (active substance in TopGun) and its saponified form (in Finalsan Plus) on the aquatic ecosystem, students of the master study program “environmental management and ecotoxicology” compared the toxicity levels of these substances using zebrafish embryos within the scope of a student research course. The project was developed by applying the 7-step problem-based learning method which allowed the students to design their experiments independently with the guidance and feedback of the lecturers. Acute toxicity was determined according to OECD test guideline 236 in D. rerio which revealed a LC50 of 1.55 mg/L of pelargonic acid, a LC50 value of 0.93 mg/L pelargonic acid in TopGun and a LC50 of 36.37 mg/L of Finalsan Plus. Neutral Red Uptake assays were performed on the rainbow trout-derived gill cell-line RTgill-W1 to determine the acute toxicity according to the OECD test guideline 129 which revealed the IC50 value of 12.4 mg/L pelargonic acid in TG. Due to solubility limits, no reliable IC50 could be obtained. The results of the tests indicate differences in the acute toxicity of the pelargonic acid-based formulations TopGun and Finalsan Plus. However, more investigations have to be done in order to analyse if the acidic form is more toxic than the saponified form. Furthermore, detailed ecotoxicological risk assessments and an evaluation of the genotoxicity of both herbicides has to be performed before any conclusion can be drawn. Financial support from the City of Vienna project PBL in Molecular Life Science (21-06) is gratefully acknowledged. KW - Environmental Chemistry KW - Ecotoxicology Y1 - 2020 ER - TY - CHAP A1 - Leitner, Rita A1 - Gepp, Barbara A1 - Liousia, Varvara A1 - Rünzler, Dominik T1 - Teaching 3R principles in ecotoxicology by performing a problem-based learning project T2 - ALTEX Proceedings, LINZ 2018 – EUSAAT 2018, Volume 7, No. 2, ISSN 2194-0479 (2018) KW - Problem Based Learning KW - Teaching KW - Ecotoxicology KW - 3R Principles Y1 - ER - TY - CHAP A1 - Fuchs, Christiane A1 - Weihs, Anna A1 - Szwarc, Dorota A1 - Mittermayr, Rainer A1 - Rünzler, Dominik A1 - Teuschl, Andreas T1 - Shock wave treatment of muscle (stem) cells - a new implementation for regeneration T2 - Proceedings of the 20th International Congress of the ISMST KW - Shockwave treatment KW - Muscle Cells KW - Regeneration Y1 - 2018 ER - TY - CHAP A1 - Maleiner, Babette A1 - Heher, Philipp A1 - Teuschl, Andreas A1 - Redl, Heinz A1 - Rünzler, Dominik A1 - Fuchs, Christiane T1 - Generation of aligned skeletal muscle-like tissue based on the application of strain to a 3D fibrin scaffold T2 - Proceedings of PACT "Designer Cells go Clinical" Symposium KW - Tissue Generation KW - Fibrin KW - Scaffold Y1 - 2018 ER - TY - CHAP A1 - Fuchs, Christiane A1 - Szwarc, Dorota A1 - Weihs, Anna A1 - Rünzler, Dominik T1 - Shock wave treatment of 3D cardiac model systems activates ERK 1/2 signaling pathway and influences cardiomyogenesis T2 - Proceedings of PACT "Designer Cells go Clinical" Symposium KW - Shockwave treatment KW - Cardiac Y1 - 2018 ER - TY - CHAP A1 - Fuchs, Christiane A1 - Szwarc, Dorota A1 - Weihs, Anna A1 - Rünzler, Dominik T1 - Shock wave treatment of 3D cardiac model systems activates ERK 1/2 signaling pathway and influences cardiomyogenesis T2 - Proceedings of LBG Meeting for Health Sciences 2016 KW - Shockwave treatment KW - Cardiac Y1 - 2018 ER - TY - CHAP A1 - Fuchs, Christiane A1 - Szwarc, Dorota A1 - Weihs, Anna A1 - Rünzler, Dominik T1 - Shock wave treatment positively influences cardiomyogenesis in an energy-dependent manner T2 - Proceedings of PACT "Designer Cells go Clinical" Symposium KW - Shockwave treatment KW - Cardiac Y1 - 2018 ER - TY - CHAP A1 - Weihs, Anna A1 - Knebl, Gerald A1 - Redl, Heinz A1 - Rünzler, Dominik T1 - Evaluation of cell migration methods in 3D hydrogels for tissue engineering applications T2 - 3. Forschungsforum der österreichischen Fachhochschulen / Fachhochschule Kärnten KW - Cells Y1 - 2019 SN - 978-3-853912850 SP - 490 EP - 491 ER - TY - CHAP A1 - Knebl, Gerald A1 - Weihs, Anna A1 - Weingant, Michaela A1 - Steininger, Thomas A1 - Redl, Heinz A1 - Rünzler, Dominik T1 - Automatisierte Datenauswertung eines Boyden-Mikro-Chemotaxis-Kammer Zell-Migrations Assays. T2 - 2. Forschungsforum der österreichischen Fachhochschulen KW - Cells KW - Data Analysis Y1 - 2019 SN - 978-3-8322-7023-0 SP - 412 EP - 417 ER - TY - CHAP A1 - Rünzler, Dominik A1 - Knebl, Gerald A1 - Peterbauer, A. A1 - Wolbank, Susanne A1 - Morton, Tatjana J. A1 - Redl, Heinz T1 - Darstellung fluoreszenzmarkierter adulter Stammzellen in drei-dimensionaler Zellkultur mittels Konfokaler Laser Scanning Mikroskopie T2 - Erstes Forschungsforum der österreichischen Fachhochschulen KW - Cells Y1 - 2019 SP - 311 EP - 316 ER - TY - CHAP A1 - Milan-Lobo, Laura A1 - Rünzler, Dominik A1 - Gaubitzer, Erwin A1 - Köhler, Gottfried A1 - Bonci, Antonello A1 - Freissmuth, Michael A1 - Sitte, Harald H. T1 - CRF receptors form dynamic complexes at the plasma membrane but only type 2 receptor increases lateral mobility after CRF binding T2 - FEBS Journal KW - Plasma Membrane Y1 - 2019 ER - TY - CHAP A1 - Kaisler, Raphaela A1 - Pázmándi, Christian A1 - Ortner, Anna A1 - Haberl, Ines A1 - Köhler, Gottfried A1 - Rünzler, Dominik A1 - Artl, Andreas A1 - Andreae, Fritz A1 - Mosgöller, Wilhelm T1 - Vasoactive intestinal peptide conjugate internalisation as strategy for cytoplasmic drug delivery T2 - Journal of Molecular Neuroscience KW - Cytplasmic Drug Delivery Y1 - 2019 ER - TY - CHAP A1 - Huber-Gries, Carina A1 - Moll, Dieter A1 - Liu, Jing A1 - Rünzler, Dominik A1 - Sára, Margit A1 - Sleytr, Uwe T1 - S-layer-streptavidin fusion proteins: Novel Tools in Nanobiotechnology T2 - 6th Annual Linz Winter Workshop - Book of Abstracts KW - Proteins KW - Nanobiology KW - Nanotechnology Y1 - 2019 SP - 11 EP - 11 ER - TY - CHAP A1 - Huber-Gries, Carina A1 - Liu, Jing A1 - Egelseer, Eva-Maria A1 - Rünzler, Dominik A1 - Sára, Margit A1 - Sleytr, Uwe T1 - Application Potential of Bacterial Self-Assembly Systems for Nanobiotechnology T2 - Nanobionics III (from Molecules to Applications) Book of Abstracts KW - Nanotechnology KW - Nanobiology KW - Bacteria Y1 - 2018 ER - TY - CHAP A1 - Huber-Gries, Carina A1 - Moll, Dieter A1 - Schlegel, Birgit A1 - Rünzler, Dominik A1 - Mader, Christoph A1 - Sára, Margit A1 - Sleytr, Uwe T1 - Recombinant S-layer-streptavidin fursion proteins: Functional protein lattices as nanoarrays for biochip development T2 - Proceedings der 28. Jahrestagung der Österreichischen Gesellschaft für Hygiene, Mikrobiologie und Präventivmedizin, Merano (Italy) KW - Proteins KW - Biochips Y1 - 2019 ER - TY - CHAP A1 - Leeb, Christine A1 - Leitner, Rita A1 - Pichler, Verena A1 - Huber-Gries, Carina A1 - Rünzler, Dominik A1 - Jesenberger, Veronika T1 - Einführung und Optimierung eines praxisorientierten PBL-Moduls im Life-Science-Bereich T2 - Proceedings des Kongresses Problem-Based Learning 2016 PBL - Kompetenzen fördern, Zukunft gestalten KW - Life Science KW - Problem Based Learning KW - Education Y1 - 2018 ER - TY - CHAP A1 - Huber-Gries, Carina A1 - Moll, Dieter A1 - Liu, Jing A1 - Rünzler, Dominik A1 - Sára, Margit A1 - Sleytr, Uwe B. T1 - Chimaeric S-Layers as Nanopatterned Biomaterials for Applications in Nanobiotechnology T2 - Poster presentation at the 7th Annual Linz Winter Workshop / Book of Abstracts / Linz KW - Biomaterial KW - Nanobiotechnology Y1 - 2019 SP - 1 EP - 10 ER - TY - CHAP A1 - Knebl, Gerald A1 - Morton, Tatjana J. A1 - Redl, Heinz A1 - Rünzler, Dominik T1 - Mechanical stimulation of cells in 3-dimensional fibrin constructs using a bioreactor T2 - 3. Forschungsforum der österreichischen Fachhochschulen / Fachhochschule Kärnten KW - Cells KW - Bioreactor Y1 - 2019 SP - 492 EP - 493 ER -