TY  - JOUR
A1  - Tomasch, Janine
A1  - Maleiner, Babette
A1  - Heher, Philipp
A1  - Rufin, Manuel
A1  - Andriotis, Orestis G.
A1  - Thurner, Philipp J.
A1  - Redl, Heinz
A1  - Fuchs, Christiane
A1  - Teuschl-Woller, Andreas H.
T1  - Changes in Elastic Moduli of Fibrin Hydrogels Within the Myogenic Range Alter Behavior of Murine C2C12 and Human C25 Myoblasts Differently
JF  - Froniers in Bioengineering and Biotechnology
N2  - Fibrin hydrogels have proven highly suitable scaffold materials for skeletal muscle tissue engineering in the past. Certain parameters of those types of scaffolds, however, greatly affect cellular mechanobiology and therefore the myogenic outcome. The aim of this study was to identify the influence of apparent elastic properties of fibrin scaffolds in 2D and 3D on myoblasts and evaluate if those effects differ between murine and human cells. Therefore, myoblasts were cultured on fibrin-coated multiwell plates (“2D”) or embedded in fibrin hydrogels (“3D”) with different elastic moduli. Firstly, we established an almost linear correlation between hydrogels’ fibrinogen concentrations and apparent elastic moduli in the range of 7.5 mg/ml to 30 mg/ml fibrinogen (corresponds to a range of 7.7–30.9 kPa). The effects of fibrin hydrogel elastic modulus on myoblast proliferation changed depending on culture type (2D vs 3D) with an inhibitory effect at higher fibrinogen concentrations in 3D gels and vice versa in 2D. The opposite effect was evident in differentiating myoblasts as shown by gene expression analysis of myogenesis marker genes and altered myotube morphology. Furthermore, culture in a 3D environment slowed down proliferation compared to 2D, with a significantly more pronounced effect on human myoblasts. Differentiation potential was also substantially impaired upon incorporation into 3D gels in human, but not in murine, myoblasts. With this study, we gained further insight in the influence of apparent elastic modulus and culture type on cellular behavior and myogenic outcome of skeletal muscle tissue engineering approaches. Furthermore, the results highlight the need to adapt parameters of 3D culture setups established for murine cells when applied to human cells.
KW  - Tissue Engineering
KW  - Fibrin
KW  - Hydrogel
KW  - Biomaterials
KW  - Cell Culture
Y1  - 
VL  - 10
SP  - 836520
ER  - 
TY  - CHAP
A1  - Maleiner, Babette
A1  - Heher, Philipp
A1  - Teuschl, Andreas
A1  - Redl, Heinz
A1  - Rünzler, Dominik
A1  - Fuchs, Christiane
T1  - Generation of aligned skeletal muscle-like tissue based on the application of strain to a 3D fibrin scaffold
T2  - Proceedings of PACT "Designer Cells go Clinical" Symposium
KW  - Tissue Generation
KW  - Fibrin
KW  - Scaffold
Y1  - 2018
ER  - 
TY  - GEN
A1  - Maleiner, Babette
A1  - Heher, Philipp
A1  - Teuschl, Andreas
A1  - Redl, Heinz
A1  - Rünzler, Dominik
A1  - Fuchs, Christiane
T1  - Generation of aligned skeletal muscle-like tissue based on the application of strain to a 3D fibrin scaffold
KW  - Tissue Generation
KW  - Fibrin
KW  - Scaffold
Y1  - 2018
ER  - 
TY  - JOUR
A1  - Teuschl, Andreas
A1  - Schuh, Christina
A1  - Halbweis, Robert
A1  - Pajer, Krisztian
A1  - Marton, Gabor
A1  - Hopf, Rudolf
A1  - Mosia, Shorena
A1  - Rünzler, Dominik
A1  - Redl, Heinz
A1  - Nogradi, Antal
A1  - Hausner, Thomas
T1  - A new preparation method for anisotropic silk fibroin nerve guidance conduits and its evaluation in vitro and in a rat sciatic nerve defect model
JF  - Tissue Engineering Part C: Methods
KW  - Fibrin
KW  - Nerve Defect
Y1  - 2018
ER  - 
TY  - GEN
A1  - Teuschl, Andreas
A1  - Fuchs, Christiane
A1  - Feichtinger, Georg
A1  - Heher, Philipp
A1  - Heimel, Patrick
A1  - Schuh, Christina
A1  - Nürnberger, Sylvia
A1  - Nau, Thomas
A1  - Rünzler, Dominik
A1  - Redl, Heinz
T1  - Fibrin or Fibroin - Not only the "o" Makes the Difference
KW  - Fibrin
KW  - Fibroin
Y1  - 2018
ER  - 
TY  - JOUR
A1  - Teuschl, Andreas
A1  - Neutsch, Lukas
A1  - Monforte Vila, Xavier
A1  - Rünzler, Dominik
A1  - van Griensven, Martijn
A1  - Gabor, Franz
A1  - Redl, Heinz
T1  - Enhanced cell adhesion on silk fibroin via lectin surface modification.
JF  - Acta Biomaterialia
KW  - Silk
KW  - Fibrin
Y1  - 
ER  - 
TY  - JOUR
A1  - Schuh, Christina
A1  - Banerjee, Asmita
A1  - Mosia, Shorena
A1  - Hopf, Rudolf
A1  - Grasl, Christian
A1  - Schima, Heinrich
A1  - Schmidhammer, Robert
A1  - Redl, Heinz
A1  - Rünzler, Dominik
A1  - Morton, Tatjana J.
T1  - Activated Schwann-like cells guided by fibrin structures enhance Axonal Regeneration
JF  - JOURNAL OF TISSUE ENGINEERING AND REGENERATIVE MEDICINE
KW  - Cells
KW  - Fibrin
Y1  - 2019
VL  - 2012
IS  - vol. 6/no. 1
ER  -