TY  - JOUR
A1  - Rothbauer, Mario
A1  - Byrne, Ruth A.
A1  - Schobesberger, Silvia
A1  - Olmos Calvo, Isabel
A1  - Fischer, Anita
A1  - Reihs, Eva I.
A1  - Spitz, Sarah
A1  - Bachmann, Barbara
A1  - Sevelda, Florian
A1  - Holinka, Johannes
A1  - Holnthoner, Wolfgang
A1  - Redl, Heinz
A1  - Toegel, Stefan
A1  - Windhager, Reinhard
A1  - Kiener, Hans P.
A1  - Ertl, Peter
T1  - Establishment of a human three-dimensional chip-based chondro-synovial coculture joint model for reciprocal cross talk studies in arthritis research
JF  - Lab on a Chip
N2  - Rheumatoid arthritis is characterised by a progressive, intermittent inflammation at the synovial membrane, which ultimately leads to the destruction of the synovial joint. The synovial membrane as the joint capsule's inner layer is lined with fibroblast-like synoviocytes that are the key player supporting persistent arthritis leading to bone erosion and cartilage destruction. While microfluidic models that model molecular aspects of bone erosion between bone-derived cells and synoviocytes have been established, RA's synovial-chondral axis has not yet been realised using a microfluidic 3D model based on human patient in vitro cultures. Consequently, we established a chip-based three-dimensional tissue coculture model that simulates the reciprocal cross talk between individual synovial and chondral organoids. When co-cultivated with synovial organoids, we could demonstrate that chondral organoids induce a higher degree of cartilage physiology and architecture and show differential cytokine response compared to their respective monocultures highlighting the importance of reciprocal tissue-level cross talk in the modelling of arthritic diseases.
KW  - Tissue Engineering
KW  - coculture joint model
KW  - arthritis
KW  - human three-dimensional chip
Y1  - 
VL  - 2021
IS  - 21
SP  - 4128
EP  - 4143
ER  -