TY - JOUR A1 - Schuh, Christina A1 - Heher, Philipp A1 - Weihs, Anna A1 - Asmita, Banerjee A1 - Wolbank, Susanne A1 - Mittermayr, Rainer A1 - Redl, Heinz A1 - Rünzler, Dominik A1 - Teuschl, Andreas T1 - Adipose derived stem cells respond to in vitro extracorporeal shockwave treatment with increased stemness and multipotency JF - New Biotechnology KW - Shockwave Y1 - ER - TY - GEN A1 - Heher, Philipp A1 - Fuchs, Christiane A1 - Prüller, Johanna A1 - Maleiner, Babette A1 - Kollmitzer, Josef A1 - Rünzler, Dominik A1 - Teuschl, Andreas A1 - Wolbank, Susanne A1 - Redl, Heinz T1 - A bioreactor-based 3D culture system for skeletal muscle engineering in fibrin scaffolds KW - Bioreactors KW - Cell Culture KW - Muscle KW - Scaffold Y1 - 2018 ER - TY - JOUR A1 - Banerjee, Asmita A1 - Nürnberger, Sylvia A1 - Hennerbichler, Simone A1 - Riedl, Stefan A1 - Schuh, Christina A1 - Hacobian, Ara A1 - Teuschl, Andreas A1 - Eibl, Jürgen A1 - Redl, Heinz T1 - In toto differentiation of human amniotic membrane towards the Schwann cell lineage JF - 227-239 KW - Membrane KW - In toto differentiation Y1 - 2018 VL - 15 IS - 2 ER - TY - JOUR A1 - Teuschl, Andreas A1 - Neutsch, Lukas A1 - Monforte Vila, Xavier A1 - Rünzler, Dominik A1 - van Griensven, Martijn A1 - Gabor, Franz A1 - Redl, Heinz T1 - Enhanced cell adhesion on silk fibroin via lectin surface modification. JF - Acta Biomaterialia KW - Silk KW - Fibrin Y1 - ER - TY - JOUR A1 - Hohlrieder, Manfred A1 - Teuschl, Andreas A1 - Cicha, Klaus A1 - van Griensven, Martijn A1 - Redl, Heinz A1 - Stampfl, Jürgen T1 - Bioreactor and scaffold design for the mechanical stimulation of anterior cruciate ligament grafts JF - Biomedical materials and engineering KW - Bioreactors KW - Ligaments KW - Grafting Y1 - 2018 VL - 23 IS - 3 SP - 225 EP - 237 ER - TY - JOUR A1 - Dungel, Peter A1 - Teuschl, Andreas A1 - Banerjee, Asmita A1 - Paier-Pourani, Jamile A1 - Redl, Heinz A1 - Kozlov, Andrey T1 - Impact of mitochondria on nitrite metabolism in HL-1 cardiomyocytes JF - Frontiers in Physiology KW - Nitrite KW - Metabolism Y1 - 2018 IS - 4 ER - TY - JOUR A1 - Teuschl, Andreas A1 - Nürnberger, Sylvia A1 - Redl, Heinz A1 - Nau, Thomas T1 - Articular cartilage tissue regeneration: current research strategies and outlook for the future JF - European Surgery KW - Tissue Regeneration KW - Cartilage Tissue Y1 - 2018 VL - 45 IS - 3 SP - 142 EP - 153 ER - TY - JOUR A1 - Teuschl, Andreas A1 - van Griensven, Martijn A1 - Redl, Heinz T1 - Sericin removal from raw Bombys mori silk scaffolds of high hierarchical order JF - Tissue Eng Part C Methods KW - Scaffold KW - Sericin Y1 - 2018 ER - TY - JOUR A1 - Schuh, Christina A1 - Banerjee, Asmita A1 - Mosia, Shorena A1 - Hopf, Rudolf A1 - Grasl, Christian A1 - Schima, Heinrich A1 - Schmidhammer, Robert A1 - Redl, Heinz A1 - Rünzler, Dominik A1 - Morton, Tatjana J. T1 - Activated Schwann-like cells guided by fibrin structures enhance Axonal Regeneration JF - JOURNAL OF TISSUE ENGINEERING AND REGENERATIVE MEDICINE KW - Cells KW - Fibrin Y1 - 2019 VL - 2012 IS - vol. 6/no. 1 ER - TY - JOUR A1 - Rieder, Bernhard A1 - Weihs, Anna A1 - Teuschl, Andreas A1 - Knebl, Gerald A1 - Kollmitzer, Josef A1 - Redl, Heinz A1 - Rünzler, Dominik T1 - Evaluation of cell response on permanent and pulsed atmospheric pressure stressed cells JF - Journal of Tissue Engineering and Regenerative Medicine KW - Cells KW - Atmospheric Pressure Y1 - 2018 VL - 1 IS - 6 SP - 240 EP - 240 ER - TY - JOUR A1 - Martin M., Frank A1 - Kollmitzer, Josef A1 - Redl, Heinz A1 - Rünzler, Dominik T1 - Shear force stimulation of adipose-tissue derived stem cells in a novel bioreactor JF - Journal of Tissue Engineering and Regenerative Medicine KW - Stem Cells KW - Adipose Y1 - 2018 VL - 1 IS - 6 SP - 340 EP - 340 ER - TY - CHAP A1 - Weihs, Anna A1 - Knebl, Gerald A1 - Redl, Heinz A1 - Rünzler, Dominik T1 - Evaluation of cell migration methods in 3D hydrogels for tissue engineering applications T2 - 3. Forschungsforum der österreichischen Fachhochschulen / Fachhochschule Kärnten KW - Cells Y1 - 2019 SN - 978-3-853912850 SP - 490 EP - 491 ER - TY - CHAP A1 - Knebl, Gerald A1 - Weihs, Anna A1 - Weingant, Michaela A1 - Steininger, Thomas A1 - Redl, Heinz A1 - Rünzler, Dominik T1 - Automatisierte Datenauswertung eines Boyden-Mikro-Chemotaxis-Kammer Zell-Migrations Assays. T2 - 2. Forschungsforum der österreichischen Fachhochschulen KW - Cells KW - Data Analysis Y1 - 2019 SN - 978-3-8322-7023-0 SP - 412 EP - 417 ER - TY - CHAP A1 - Rünzler, Dominik A1 - Knebl, Gerald A1 - Peterbauer, A. A1 - Wolbank, Susanne A1 - Morton, Tatjana J. A1 - Redl, Heinz T1 - Darstellung fluoreszenzmarkierter adulter Stammzellen in drei-dimensionaler Zellkultur mittels Konfokaler Laser Scanning Mikroskopie T2 - Erstes Forschungsforum der österreichischen Fachhochschulen KW - Cells Y1 - 2019 SP - 311 EP - 316 ER - TY - JOUR A1 - Teuschl, Andreas A1 - Aigner, Elmar A1 - Hohlrieder, Martin A1 - Cicha, Klaus A1 - Stampfl, Jürgen A1 - Redl, Heinz T1 - Stimulation of ligament tissue formation on a silk scaffold with mechanical loading using a custom-made bioreactor system JF - Journal of Tissue Engineering and Regenerative Medicine KW - Ligament KW - Tissue Formation KW - Scaffold KW - Silk KW - Bioreactor Y1 - 2018 VL - 1 IS - 6 SP - 51 EP - 51 ER - TY - JOUR A1 - Teuschl, Andreas A1 - Ferguson, James A1 - Szomolanyi, Pavol A1 - Trattnig, Siegfried A1 - Redl, Heinz A1 - Nau, Thomas T1 - Osteointegration of anterior cruciate ligament scaffolds fabricated of bombyx mori silk JF - Journal of Tissue Engineering and Regenerative Medicine KW - Osteointegration KW - Cruciate Ligament KW - Scaffold KW - Silk Y1 - 2018 VL - 1 IS - 6 SP - 181 EP - 182 ER - TY - JOUR A1 - Rieder, Bernhard A1 - Weihs, Anna A1 - Weidinger, Adelheid A1 - Sczwarc, Dorota A1 - Nürnberger, Sylvia A1 - Redl, Heinz A1 - Rünzler, Dominik A1 - Huber-Gries, Carina A1 - Teuschl, Andreas T1 - Hydrostatic pressure-generated reactive oxygen species induce osteoarthritic conditions in cartilage pellet cultures JF - Scientific Reports KW - Bioreactor KW - Osteoarthritis KW - Cartilage KW - Reactive oxygen species Y1 - ER - TY - GEN A1 - Mühleder, Severin A1 - Fuchs, Christiane A1 - Bassilio, Jose A1 - Sczwarc, Dorota A1 - Pill, Karoline A1 - Slezak, Paul A1 - Labuda, Krystina A1 - Siehs, Christian A1 - Pröll, Johannes A1 - Priglinger, Eleni A1 - Redl, Heinz A1 - Holnthoner, Wolfgang T1 - The purinergic receptor P2Y2 modulates endothelial sprouting and angiogenesis KW - Angiogenesis Y1 - 2018 ER - TY - JOUR A1 - Nürnberger, S. A1 - Schneider, C. A1 - Keibl, C. A1 - Schädl, Barbara A1 - Heimel, P. A1 - Monforte, X. A1 - Teuschl, A. H. A1 - Nalbach, M. A1 - Thurner, P. J. A1 - Grillari, J. A1 - Redl, Heinz A1 - Wolbank, S. T1 - Repopulation of decellularised articular cartilage by laser-based matrix engraving JF - EBioMedicine. N2 - Background: In spite of advances in the treatment of cartilage defects using cell and scaffold-based therapeutic strategies, the long-term outcome is still not satisfying since clinical scores decline years after treatment. Scaffold materials currently used in clinical settings have shown limitations in providing suitable biomechanical properties and an authentic and protective environment for regenerative cells. To tackle this problem, we developed a scaffold material based on decellularised human articular cartilage. Methods: Human articular cartilage matrix was engraved using a CO2 laser and treated for decellularisation and glycosaminoglycan removal. Characterisation of the resulting scaffold was performed via mechanical testing, DNA and GAG quantification and in vitro cultivation with adipose-derived stromal cells (ASC). Cell vitality, adhesion and chondrogenic differentiation were assessed. An ectopic, unloaded mouse model was used for the assessment of the in vivo performance of the scaffold in combination with ASC and human as well as bovine chondrocytes. The novel scaffold was compared to a commercial collagen type I/III scaffold. Findings: Crossed line engravings of the matrix allowed for a most regular and ubiquitous distribution of cells and chemical as well as enzymatic matrix treatment was performed to increase cell adhesion. The biomechanical characteristics of this novel scaffold that we term CartiScaff were found to be superior to those of commercially available materials. Neo-tissue was integrated excellently into the scaffold matrix and new collagen fibres were guided by the laser incisions towards a vertical alignment, a typical feature of native cartilage important for nutrition and biomechanics. In an ectopic, unloaded in vivo model, chondrocytes and mesenchymal stromal cells differentiated within the incisions despite the lack of growth factors and load, indicating a strong chondrogenic microenvironment within the scaffold incisions. Cells, most noticeably bone marrow-derived cells, were able to repopulate the empty chondrocyte lacunae inside the scaffold matrix. Interpretation: Due to the better load-bearing, its chondrogenic effect and the ability to guide matrix-deposition, CartiScaff is a promising biomaterial to accelerate rehabilitation and to improve long term clinical success of cartilage defect treatment. Funding: Austrian Research Promotion Agency FFG ("CartiScaff" #842455), Lorenz Böhler Fonds (16/13), City of Vienna Competence Team Project Signaltissue (MA23, #18-08). Keywords: Cartilage regeneration; Decellularisation; Ectopic animal model; Laser engraving; Mechanical testing; Repopulation. KW - Tissue Engineering KW - Cartilage regeneration KW - Mechanical Testing KW - Decellularization KW - Biomaterials Y1 - 2021 VL - 64 IS - 103196. ER - TY - JOUR A1 - Schneider, Karl Heinrich A1 - Enayati, Marjan A1 - Grasl, Christian A1 - Walter, Ingrid A1 - Budinsky, Lubos A1 - Zebic, Gabriel A1 - Kaun, Christoph A1 - Wagner, Anja A1 - Kratochwill, Klaus A1 - Redl, Heinz A1 - Teuschl, Andreas A1 - Podesser, Bruno K. A1 - Bergmeister, Helga T1 - Acellular vascular matrix grafts from human placenta chorion: Impact of ECM preservation on graft characteristics, protein composition and in vivo performance. JF - Biomaterials N2 - Small diameter vascular grafts from human placenta, decellularized with either Triton X-100 (Triton) or SDS and crosslinked with heparin were constructed and characterized. Graft biochemical properties, residual DNA, and protein composition were evaluated to compare the effect of the two detergents on graft matrix composition and structural alterations. Biocompatibility was tested in vitro by culturing the grafts with primary human macrophages and in vivo by subcutaneous implantation of graft conduits (n = 7 per group) into the flanks of nude rats. Subsequently, graft performance was evaluated using an aortic implantation model in Sprague Dawley rats (one month, n = 14). In situ graft imaging was performed using MRI angiography. Retrieved specimens were analyzed by electromyography, scanning electron microscopy, histology and immunohistochemistry to evaluate cell migration and the degree of functional tissue remodeling. Both decellularization methods resulted in grafts of excellent biocompatibility in vitro and in vivo, with low immunogenic potential. Proteomic data revealed removal of cytoplasmic proteins with relative enrichment of ECM proteins in decelluarized specimens of both groups. Noteworthy, LC-Mass Spectrometry analysis revealed that 16 proteins were exclusively preserved in Triton decellularized specimens in comparison to SDS-treated specimens. Aortic grafts showed high patency rates, no signs of thrombus formation, aneurysms or rupture. Conduits of both groups revealed tissue-specific cell migration indicative of functional remodeling. This study strongly suggests that decellularized allogenic grafts from the human placenta have the potential to be used as vascular replacement materials. Both detergents produced grafts with low residual immunogenicity and appropriate mechanical properties. Observed differences in graft characteristics due to preservation method had no impact on successful in vivo performance in the rodent model. KW - Biomaterial KW - Tissue Engineering Y1 - SP - 14 EP - 26 ER -