TY  - JOUR
A1  - Purtscher, Michaela
A1  - Rothbauer, Mario
A1  - Kratz, Sebastian Rudi Adam
A1  - Bailey, Andrew
A1  - Lieberzeit, Peter
A1  - Ertl, Peter
T1  - A microfluidic impedance-based extended infectivity assay: combining retroviral amplification and cytopathic effect monitoring on a single lab-on-a-chip platform
JF  - Lab on a Chip
N2  - Detection, quantification and monitoring of virus – host cell interactions are of great importance when evaluating the safety of pharmaceutical products. With the wide usage of viral based vector systems in combination with mammalian cell lines for the production of biopharmaceuticals, the presence of replication competent viral particles needs to be avoided and potential hazards carefully assessed. Consequently, regulatory agencies recommend viral clearance studies using plaque assays or TCID50 assays to evaluate the efficiency of the production process in removing viruses. While plaque assays provide reliable information on the presence of viral contaminations, they are still tedious to perform and can take up to two weeks to finish. To overcome some of these limitations, we have automated, miniaturized and integrated the dual cell culture bioassay into a common lab-on-a-chip platform containing embedded electrical sensor arrays to enrich and detect infectious viruses. Results of our microfluidic single step assay show that a significant reduction in assay time down to 3 to 4 days can be achieved using simultaneous cell-based viral amplification, release and detection of cytopathic effects in a target cell line. We further demonstrate the enhancing effect of continuous fluid flow on infection of PG-4 reporter cells by newly formed and highly active virions by M. dunni cells, thus pointing to the importance of physical relevant viral–cell interactions.
KW  - Tissue Engineering
KW  - Microfluidics
KW  - Lab on Chip
Y1  - 2021
VL  - 2021
IS  - Issue 7
SP  - 1364
EP  - 1372
ER  - 
TY  - JOUR
A1  - Bachmann, Barbara
A1  - Spitz, Sarah
A1  - Rothbauer, Mario
A1  - Jordan, Christian
A1  - Purtscher, Michaela
A1  - Zirath, Helene
A1  - Schuller, Patrick
A1  - Eilenberger, Christoph
A1  - Ali, Syed Faheem
A1  - Mühleder, Severin
A1  - Priglinger, Eleni
A1  - Harasek, Michael
A1  - Redl, Heinz
A1  - Holnthoner, Wolfgang
A1  - Ertl, Peter
T1  - Engineering of three-dimensional pre-vascular networks within fibrin hydrogel constructs by microfluidic control over reciprocal cell signaling
JF  - Biomicrofluidics
KW  - Microfluidic
KW  - Vascularization
KW  - Tissue Engineering
Y1  - 2019
ER  - 
TY  - JOUR
A1  - Rothbauer, Mario
A1  - Byrne, Ruth A.
A1  - Schobesberger, Silvia
A1  - Olmos Calvo, Isabel
A1  - Fischer, Anita
A1  - Reihs, Eva I.
A1  - Spitz, Sarah
A1  - Bachmann, Barbara
A1  - Sevelda, Florian
A1  - Holinka, Johannes
A1  - Holnthoner, Wolfgang
A1  - Redl, Heinz
A1  - Toegel, Stefan
A1  - Windhager, Reinhard
A1  - Kiener, Hans P.
A1  - Ertl, Peter
T1  - Establishment of a human three-dimensional chip-based chondro-synovial coculture joint model for reciprocal cross talk studies in arthritis research
JF  - Lab on a Chip
N2  - Rheumatoid arthritis is characterised by a progressive, intermittent inflammation at the synovial membrane, which ultimately leads to the destruction of the synovial joint. The synovial membrane as the joint capsule's inner layer is lined with fibroblast-like synoviocytes that are the key player supporting persistent arthritis leading to bone erosion and cartilage destruction. While microfluidic models that model molecular aspects of bone erosion between bone-derived cells and synoviocytes have been established, RA's synovial-chondral axis has not yet been realised using a microfluidic 3D model based on human patient in vitro cultures. Consequently, we established a chip-based three-dimensional tissue coculture model that simulates the reciprocal cross talk between individual synovial and chondral organoids. When co-cultivated with synovial organoids, we could demonstrate that chondral organoids induce a higher degree of cartilage physiology and architecture and show differential cytokine response compared to their respective monocultures highlighting the importance of reciprocal tissue-level cross talk in the modelling of arthritic diseases.
KW  - Tissue Engineering
KW  - coculture joint model
KW  - arthritis
KW  - human three-dimensional chip
Y1  - 
VL  - 2021
IS  - 21
SP  - 4128
EP  - 4143
ER  -