TY - GEN A1 - Teuschl, Andreas A1 - Fuchs, Christiane T1 - Bioreactors in Musculoskeletal Tissue Engineering KW - Bioreactor KW - Tissue Engineering Y1 - ER - TY - GEN A1 - Küenburg, Bernhard A1 - Teuschl, Andreas T1 - Regenerative silk ligament: scale up and regulatory strategy of a textile engineered silk implant for tissue regeneration of injured human ACL (anterior cruciate ligament) N2 - 61 a93591 27-SY-1 Regenerative silk ligament: scale up and regulatory strategy of a textile engineered silk implant for tissue regeneration of injured human ACL (anterior cruciate ligament) Bernhard Küenburg，Andreas Teuschl MorphoMed, Austria In the USA around 370.000 annual ACL ruptures in the predominately young population (age 18-30) following sport injuries cause a lot of pain and long term troubles for the affected patients. The well established standard procedure of autologous tissue transplantation stabilizes the knee and allows sport activities after 9-12 months. However, harvest of autologous tissue such as patellar tendon or semitendinosus (the most frequently used autograft) weakens other body areas and long term data show an increased rate of arthroses (up to 50% after 10 years) associated with ACL reconstructions. Based on numerous preclinical data and data from a 12-month sheep study the scale-up as well as regulatory approval process of a novel textile engineered silk implant as ACL graft have been initiated. It is planned to enter a clinical trial and strive for regulatory approval as a medical class 3 implant. The sheep data (1) have revealed an approximately 50% degradation of the purified medical silk, which acts as scaffold for the regrowth of a new endogenous ligament. The formation of oriented collagenous tissue fibers including vascularization proves a ligament tissue regeneration for the first time. Scale-up and implementation of a commercial process require a defined set of specifications of commercial silkworm (Bombyx mori) silk, a biological raw material sourced from the textile industry as well as adequate analytical methods to characterize the depletion of sericin in the course of the production process. Based on first full scale samples, the defined biocompatibility program has to be executed, in the EU following the ISO requirements of the notified bodies, in the USA determined by the FDA. Upon submission of the full technical documentation as well as the biocompati-bility data, approval of a clinical trial can be achieved in order to demonstrate the clinical efficacy of the silk based ACL graft compared to the gold standard method in two different randomized groups. As primary endpoint the knee stability will be tested by an apparatus supported Lachmann test. In this study, besides the clinical benefit the patient safety is an important goal. The results of this study will be used for achievement of regulatory approval. References: 1) Teuschl A, Heimel P, Nürnberger S, van Griensven M, Redl H, Nau T. Am. J. Sports Med. 2016;44:1547–57. 2) Teuschl AH, van Griensven M, Redl H. Tissue Eng. Part C Methods. 2014;20:431–9. KW - Biomaterial KW - Ligament Y1 - ER - TY - GEN A1 - Teuschl, Andreas A1 - Schuh, Christina A1 - Weihs, Anna A1 - Guillaume, Olivier A1 - Monforte Vila, Xavier A1 - Redl, Heinz A1 - Kaplan, David A1 - Rünzler, Dominik T1 - Tailoring bioactivity of silk-based biomaterials via delivering and functionalization strategies with fibrinogen/thrombin, plant lectins or laminin KW - Biomaterials KW - Tissue Engineering KW - Silk Y1 - ER - TY - GEN A1 - Teuschl, Andreas A1 - Heimel, Patrick A1 - Monforte Vila, Xavier A1 - Nürnberger, Sylvia A1 - Tangl, Stefan A1 - van Griensven, Martijn A1 - Redl, Heinz A1 - Nau, Thomas T1 - Anterior cruciate ligament regeneration using the silk-based RegACL scaffold KW - Tissue Engineering KW - Silk KW - Biomaterial KW - Anterior Cruciate Ligament Y1 - ER - TY - GEN A1 - Salzer, Elias A1 - Rieder, Bernhard A1 - Monforte Vila, Xavier A1 - Weihs, Anna A1 - Rünzler, Dominik A1 - Teuschl, Andreas T1 - Evaluation of a novel hydrostatic pressure bioreactor on bovine cartilage chips KW - Bioreactor KW - Tissue Engineering KW - Cartilage Y1 - ER - TY - GEN A1 - Fuchs, Christiane A1 - Weihs, Anna A1 - Szwarc, Dorota A1 - Mittermayr, Rainer A1 - Rünzler, Dominik A1 - Teuschl, Andreas T1 - Shock wave treatment of muscle (stem) cells - a new implementation for regeneration KW - Shockwave treatment KW - Muscle Cells KW - Regeneration Y1 - 2018 ER - TY - GEN A1 - Teuschl, Andreas A1 - Weihs, Anna A1 - Fuchs, Christiane A1 - Monforte Vila, Xavier T1 - Silk as a versatile biomaterial for musculoskeletal tissue engineering KW - Silk KW - Biomaterials Y1 - 2018 ER - TY - GEN A1 - Szwarc, Dorota A1 - Fuchs, Christiane A1 - Weihs, Anna A1 - Monforte Vila, Xavier A1 - Hanetseder, Dominik A1 - Teuschl, Andreas A1 - Rünzler, Dominik T1 - The effect of shock waves on in vitro cartilage development in silk scaffolds KW - Shockwave treatment KW - In Vitro KW - Cartilage KW - Silk Y1 - 2018 ER - TY - GEN A1 - Maleiner, Babette A1 - Heher, Philipp A1 - Teuschl, Andreas A1 - Redl, Heinz A1 - Rünzler, Dominik A1 - Fuchs, Christiane T1 - Generation of aligned skeletal muscle-like tissue based on the application of strain to a 3D fibrin scaffold KW - Tissue Generation KW - Fibrin KW - Scaffold Y1 - 2018 ER - TY - GEN A1 - Teuschl, Andreas A1 - Fuchs, Christiane A1 - Weihs, Anna A1 - Heimel, Patrick A1 - Rünzler, Dominik A1 - Redl, Heinz A1 - Nau, Thomas T1 - The Silk Road from Textiles to Novel Medical Implants KW - Silk Y1 - 2018 ER - TY - GEN A1 - Teuschl, Andreas A1 - Heimel, Patrick A1 - Nürnberger, Sylvia A1 - Redl, Heinz A1 - Nau, Thomas T1 - ACL Regeneration using a novel silk fiber based scaffold - Histological Results of a Large Animal Study KW - Tissue Regeneration KW - Silk KW - Scaffold Y1 - 2018 ER - TY - GEN A1 - Teuschl, Andreas T1 - Silk Processing - from Gels to ACL Replacement KW - Silk Y1 - 2018 ER - TY - GEN A1 - Teuschl, Andreas A1 - Schuh, Christina A1 - Halbweis, Robert A1 - Marton, Gábor A1 - Pajer, Krisztián A1 - Hopf, Rudolf A1 - Mosia, Shorena A1 - Rünzler, Dominik A1 - Nógrádi, Antal A1 - Hausner, Thomas A1 - Redl, Heinz T1 - Silk fibroin for peripheral nerve regeneration: a novel preparation method improved mechanical characteristics and supports regeneration in rat sciatic nerves KW - Fibroin KW - Regeneration Y1 - 2018 ER - TY - GEN A1 - Fuchs, Christiane A1 - Weihs, Anna A1 - Teuschl, Andreas A1 - Hartinger, Joachim A1 - Slezak, Paul A1 - Mittermayr, Rainer A1 - Redl, Heinz A1 - Junger, Wolfgang A1 - Sitte, Harald A1 - Rünzler, Dominik T1 - Shockwave Treatment Enhances Proliferation and Improves Wound Healing via Purinergic Signaling Linked ERK 1/2 Pathways KW - Shockwave treatment KW - Healing Processes KW - Signaling Y1 - 2018 ER - TY - GEN A1 - Fuchs, Christiane A1 - Weihs, Anna A1 - Teuschl, Andreas A1 - Hartinger, Joachim A1 - Slezak, Paul A1 - Mittermayr, Rainer A1 - Redl, Heinz A1 - Junger, Wolfgang A1 - Sitte, Harald A1 - Rünzler, Dominik T1 - Shockwave Treatment Augments Proliferation and Improves Wound Healing via Purinergic Signaling Linked ERK 1/2 Pathways KW - Shockwave treatment KW - Healing Processes KW - Signaling Y1 - 2018 ER - TY - GEN A1 - Weihs, Anna A1 - Fuchs, Christiane A1 - Teuschl, Andreas A1 - Hartinger, Joachim A1 - Slezak, Paul A1 - Mittermayr, Rainer A1 - Redl, Heinz A1 - Junger, Wolfgang A1 - Sitte, Harald A1 - Rünzler, Dominik T1 - Shockwave treatment activates Erk1/2 pathways predominantly via P2Y receptor involvement KW - Shockwave Y1 - ER - TY - GEN A1 - Teuschl, Andreas A1 - Fuchs, Christiane A1 - Feichtinger, Georg A1 - Heher, Philipp A1 - Heimel, Patrick A1 - Schuh, Christina A1 - Nürnberger, Sylvia A1 - Nau, Thomas A1 - Rünzler, Dominik A1 - Redl, Heinz T1 - Fibrin or Fibroin - Not only the "o" Makes the Difference KW - Fibrin KW - Fibroin Y1 - 2018 ER - TY - GEN A1 - Teuschl, Andreas A1 - Nürnberger, Sylvia A1 - Heimel, Patrick A1 - Redl, Heinz A1 - Nau, Thomas T1 - Regeneration of the Anterior Cruciate Ligament Using a Silk-Fiber Based Scaffold - Histological Results KW - Tissue Regeneration KW - Ligaments KW - Scaffold Y1 - 2018 ER - TY - GEN A1 - Heher, Philipp A1 - Fuchs, Christiane A1 - Prüller, Johanna A1 - Maleiner, Babette A1 - Kollmitzer, Josef A1 - Rünzler, Dominik A1 - Teuschl, Andreas A1 - Wolbank, Susanne A1 - Redl, Heinz T1 - A bioreactor-based 3D culture system for skeletal muscle engineering in fibrin scaffolds KW - Bioreactors KW - Cell Culture KW - Muscle KW - Scaffold Y1 - 2018 ER - TY - GEN A1 - Hromada, Carina A1 - Tomasch, Janine A1 - Weihs, Anna A1 - Rünzler, Dominik A1 - Teuschl, Andreas T1 - Engineering of 3D Tissue Constructs Using our Novel MagneTissue Bioreactor as Alternatives to Animal Models KW - Bioreactor KW - Biomaterials Y1 - ER - TY - GEN A1 - Schneider, Karl Heinrich A1 - Aigner, Petra A1 - Monforte Vila, Xavier A1 - Holnthoner, Wolfgang A1 - Teuschl, Andreas A1 - Bergmeister, Helga A1 - Redl, Heinz T1 - Naturally derived acellular small diameter vascular grafts from human placenta for reconstructive surgery KW - Placenta KW - Grafting KW - Surgery Y1 - 2018 ER -