TY  - JOUR
A1  - Ziadlou, Reihane
A1  - Rotman, Stijn
A1  - Teuschl, Andreas
A1  - Salzer, Elias
A1  - Barbero, Andrea
A1  - Martin, Ivan
A1  - Alini, Mauro
A1  - Eglin, David
A1  - Grad, Sibylle
T1  - Optimization of hyaluronic acid-tyramine/silk-fibroin composite hydrogels for cartilage tissue engineering and delivery of anti-inflammatory and anabolic drugs
JF  - Materials Science and Engineering: C
N2  - Injury of articular cartilage leads to an imbalance in tissue homeostasis, and due to the poor self-healing capacity of cartilage the affected tissue often exhibits osteoarthritic changes. In recent years, injectable and highly tunable composite hydrogels for cartilage tissue engineering and drug delivery have been introduced as a desirable alternative to invasive treatments. In this study, we aimed to formulate injectable hydrogels for drug delivery and cartilage tissue engineering by combining different concentrations of hyaluronic acid-tyramine (HA-Tyr) with regenerated silk-fibroin (SF) solutions. Upon enzymatic crosslinking, the gelation and mechanical properties were characterized over time. To evaluate the effect of the hydrogel compositions and properties on extracellular matrix (ECM) deposition, bovine chondrocytes were embedded in enzymatically crosslinked HA-Tyr/SF composites (in further work abbreviated as HA/SF) or HA-Tyr hydrogels. We demonstrated that all hydrogel formulations were cytocompatible and could promote the expression of cartilage matrix proteins allowing chondrocytes to produce ECM, while the most prominent chondrogenic effects were observed in hydrogels with HA20/SF80 polymeric ratios. Unconfined mechanical testing showed that the compressive modulus for HA20/SF80 chondrocyte-laden constructs was increased almost 10-fold over 28 days of culture in chondrogenic medium which confirmed the superior production of ECM in this hydrogel compared to other hydrogels in this study. Furthermore, in hydrogels loaded with anabolic and anti-inflammatory drugs, HA20/SF80 hydrogel showed the longest and the most sustained release profile over time which is desirable for the long treatment duration typically necessary for osteoarthritic joints. In conclusion, HA20/SF80 hydrogel was successfully established as a suitable injectable biomaterial for cartilage tissue engineering and drug delivery applications.
KW  - Tissue Engineering
KW  - Cartilage
KW  - Mechanical Testing
KW  - Biomaterials
KW  - Chondrocytes
Y1  - 
VL  - 120
IS  - 111701
ER  - 
TY  - GEN
A1  - Salzer, Elias
A1  - Rieder, Bernhard
A1  - Monforte Vila, Xavier
A1  - Weihs, Anna
A1  - Rünzler, Dominik
A1  - Teuschl, Andreas
T1  - Evaluation of a novel hydrostatic pressure bioreactor on bovine cartilage chips
KW  - Bioreactor
KW  - Tissue Engineering
KW  - Cartilage
Y1  - 
ER  - 
TY  - JOUR
A1  - Schneider, Cornelia
A1  - Lehmann, Johannes
A1  - van Osch, Gerjo
A1  - Hildner, Florian
A1  - Teuschl, Andreas
A1  - Monforte Vila, Xavier
A1  - Miosga, David
A1  - Heimel, Patrick
A1  - Priglinger, Eleni
A1  - Redl, Heinz
A1  - Wolbank, Susanne
A1  - Nürnberger, Sylvia
T1  - Systematic Comparison of Protocols for the Preparation of Human Articular Cartilage for Use as Scaffold Material in Cartilage Tissue Engineering
JF  - Tissue Eng Part C Methods
KW  - Cartilage
KW  - Scaffold Material
Y1  - 2018
VL  - 22
IS  - 12
ER  - 
TY  - GEN
A1  - Szwarc, Dorota
A1  - Fuchs, Christiane
A1  - Weihs, Anna
A1  - Monforte Vila, Xavier
A1  - Hanetseder, Dominik
A1  - Teuschl, Andreas
A1  - Rünzler, Dominik
T1  - The effect of shock waves on in vitro cartilage development in silk scaffolds
KW  - Shockwave treatment
KW  - In Vitro
KW  - Cartilage
KW  - Silk
Y1  - 2018
ER  - 
TY  - JOUR
A1  - Rieder, Bernhard
A1  - Weihs, Anna
A1  - Weidinger, Adelheid
A1  - Sczwarc, Dorota
A1  - Nürnberger, Sylvia
A1  - Redl, Heinz
A1  - Rünzler, Dominik
A1  - Huber-Gries, Carina
A1  - Teuschl, Andreas
T1  - Hydrostatic pressure-generated reactive oxygen species induce osteoarthritic conditions in cartilage pellet cultures
JF  - Scientific Reports
KW  - Bioreactor
KW  - Osteoarthritis
KW  - Cartilage
KW  - Reactive oxygen species
Y1  - 
ER  - 
TY  - JOUR
A1  - Nürnberger, Sylvia
A1  - Schneider, Cornelia
A1  - van Osch, Gerjo
A1  - Keibl, Claudia
A1  - Rieder, Bernhard
A1  - Monforte, Xavier
A1  - Teuschl, Andreas
A1  - Mühleder, Severin
A1  - Holnthoner, Wolfgang
A1  - Schädl, Barbara
A1  - Gahleitner, Christoph
A1  - Redl, Heinz
A1  - Wolbank, Susanne
T1  - Repopulation of an auricular cartilage scaffold, AuriScaff, perforated with an enzyme combination.
JF  - Acta Biomaterialia
KW  - Tissue Engineering
KW  - Decellularization
KW  - Cartilage
Y1  - 
ER  -