TY  - GEN
A1  - Salzer, Elias
A1  - Rieder, Bernhard
A1  - Monforte Vila, Xavier
A1  - Weihs, Anna
A1  - Rünzler, Dominik
A1  - Teuschl, Andreas
T1  - Evaluation of a novel hydrostatic pressure bioreactor on bovine cartilage chips
KW  - Bioreactor
KW  - Tissue Engineering
KW  - Cartilage
Y1  - 
ER  - 
TY  - JOUR
A1  - Hackethal, Johannes
A1  - Mühleder, Severin
A1  - Hofer, Alexandra
A1  - Schneider, Karl Heinrich
A1  - Prüller, Johanna
A1  - Hennerbichler, Simone
A1  - Redl, Heinz
A1  - Teuschl, Andreas
T1  - An Effective Method of Atelocollagen Type 1/3 Isolation from Human Placenta and Its In Vitro Characterization in Two-Dimensional and Three-Dimensional Cell Culture Applications
JF  - Tissue Eng Part C Methods
KW  - Placenta
KW  - In Vitro
KW  - Cell Culture
Y1  - 2018
VL  - 23
IS  - 5
SP  - 274
EP  - 285
ER  - 
TY  - CHAP
A1  - Fuchs, Christiane
A1  - Weihs, Anna
A1  - Szwarc, Dorota
A1  - Mittermayr, Rainer
A1  - Rünzler, Dominik
A1  - Teuschl, Andreas
T1  - Shock wave treatment of muscle (stem) cells - a new implementation for regeneration
T2  - Proceedings of the 20th International Congress of the ISMST
KW  - Shockwave treatment
KW  - Muscle Cells
KW  - Regeneration
Y1  - 2018
ER  - 
TY  - JOUR
A1  - Berkovitch, Yulia
A1  - Cohen, Talia
A1  - Peled, Eli
A1  - Schmidhammer, Robert
A1  - Hildner, Florian
A1  - Teuschl, Andreas
A1  - Wolbank, Susanne
A1  - Yelin, Dvir
A1  - Redl, Heinz
A1  - Seliktar, Dror
T1  - Hydrogel Composition and Laser Micro-Patterning to Regulate Sciatic Nerve Regeneration
JF  - Journal of Tissue Engineering and Regenerative Medicine
KW  - Micro-Patterning
KW  - Laser
KW  - Nerve Regeneration
Y1  - 2018
VL  - 12
IS  - 4
ER  - 
TY  - GEN
A1  - Fuchs, Christiane
A1  - Weihs, Anna
A1  - Szwarc, Dorota
A1  - Mittermayr, Rainer
A1  - Rünzler, Dominik
A1  - Teuschl, Andreas
T1  - Shock wave treatment of muscle (stem) cells - a new implementation for regeneration
KW  - Shockwave treatment
KW  - Muscle Cells
KW  - Regeneration
Y1  - 2018
ER  - 
TY  - GEN
A1  - Teuschl, Andreas
A1  - Weihs, Anna
A1  - Fuchs, Christiane
A1  - Monforte Vila, Xavier
T1  - Silk as a versatile biomaterial for musculoskeletal tissue engineering
KW  - Silk
KW  - Biomaterials
Y1  - 2018
ER  - 
TY  - JOUR
A1  - Schneider, Cornelia
A1  - Lehmann, Johannes
A1  - van Osch, Gerjo
A1  - Hildner, Florian
A1  - Teuschl, Andreas
A1  - Monforte Vila, Xavier
A1  - Miosga, David
A1  - Heimel, Patrick
A1  - Priglinger, Eleni
A1  - Redl, Heinz
A1  - Wolbank, Susanne
A1  - Nürnberger, Sylvia
T1  - Systematic Comparison of Protocols for the Preparation of Human Articular Cartilage for Use as Scaffold Material in Cartilage Tissue Engineering
JF  - Tissue Eng Part C Methods
KW  - Cartilage
KW  - Scaffold Material
Y1  - 2018
VL  - 22
IS  - 12
ER  - 
TY  - CHAP
A1  - Maleiner, Babette
A1  - Heher, Philipp
A1  - Teuschl, Andreas
A1  - Redl, Heinz
A1  - Rünzler, Dominik
A1  - Fuchs, Christiane
T1  - Generation of aligned skeletal muscle-like tissue based on the application of strain to a 3D fibrin scaffold
T2  - Proceedings of PACT "Designer Cells go Clinical" Symposium
KW  - Tissue Generation
KW  - Fibrin
KW  - Scaffold
Y1  - 2018
ER  - 
TY  - GEN
A1  - Szwarc, Dorota
A1  - Fuchs, Christiane
A1  - Weihs, Anna
A1  - Monforte Vila, Xavier
A1  - Hanetseder, Dominik
A1  - Teuschl, Andreas
A1  - Rünzler, Dominik
T1  - The effect of shock waves on in vitro cartilage development in silk scaffolds
KW  - Shockwave treatment
KW  - In Vitro
KW  - Cartilage
KW  - Silk
Y1  - 2018
ER  - 
TY  - GEN
A1  - Maleiner, Babette
A1  - Heher, Philipp
A1  - Teuschl, Andreas
A1  - Redl, Heinz
A1  - Rünzler, Dominik
A1  - Fuchs, Christiane
T1  - Generation of aligned skeletal muscle-like tissue based on the application of strain to a 3D fibrin scaffold
KW  - Tissue Generation
KW  - Fibrin
KW  - Scaffold
Y1  - 2018
ER  - 
TY  - GEN
A1  - Teuschl, Andreas
A1  - Fuchs, Christiane
A1  - Weihs, Anna
A1  - Heimel, Patrick
A1  - Rünzler, Dominik
A1  - Redl, Heinz
A1  - Nau, Thomas
T1  - The Silk Road from Textiles to Novel Medical Implants
KW  - Silk
Y1  - 2018
ER  - 
TY  - GEN
A1  - Teuschl, Andreas
A1  - Heimel, Patrick
A1  - Nürnberger, Sylvia
A1  - Redl, Heinz
A1  - Nau, Thomas
T1  - ACL Regeneration using a novel silk fiber based scaffold - Histological Results of a Large Animal Study
KW  - Tissue Regeneration
KW  - Silk
KW  - Scaffold
Y1  - 2018
ER  - 
TY  - GEN
A1  - Teuschl, Andreas
T1  - Silk Processing - from Gels to ACL Replacement
KW  - Silk
Y1  - 2018
ER  - 
TY  - JOUR
A1  - Heher, Philipp
A1  - Maleiner, Babette
A1  - Prüller, Johanna
A1  - Teuschl, Andreas
A1  - Kollmitzer, Josef
A1  - Monforte Vila, Xavier
A1  - Wolbank, Susanne
A1  - Redl, Heinz
A1  - Rünzler, Dominik
A1  - Fuchs, Christiane
T1  - A novel bioreactor for the generation of highly aligned 3D skeletal muscle-like constructs through orientation of fibrin via application of static strain
JF  - Acta Biomaterialia
KW  - Bioreactor
Y1  - 
ER  - 
TY  - JOUR
A1  - Teuschl, Andreas
A1  - Schuh, Christina
A1  - Halbweis, Robert
A1  - Pajer, Krisztian
A1  - Marton, Gabor
A1  - Hopf, Rudolf
A1  - Mosia, Shorena
A1  - Rünzler, Dominik
A1  - Redl, Heinz
A1  - Nogradi, Antal
A1  - Hausner, Thomas
T1  - A new preparation method for anisotropic silk fibroin nerve guidance conduits and its evaluation in vitro and in a rat sciatic nerve defect model
JF  - Tissue Engineering Part C: Methods
KW  - Fibrin
KW  - Nerve Defect
Y1  - 2018
ER  - 
TY  - GEN
A1  - Teuschl, Andreas
A1  - Schuh, Christina
A1  - Halbweis, Robert
A1  - Marton, Gábor
A1  - Pajer, Krisztián
A1  - Hopf, Rudolf
A1  - Mosia, Shorena
A1  - Rünzler, Dominik
A1  - Nógrádi, Antal
A1  - Hausner, Thomas
A1  - Redl, Heinz
T1  - Silk fibroin for peripheral nerve regeneration: a novel preparation method improved mechanical characteristics and supports regeneration in rat sciatic nerves
KW  - Fibroin
KW  - Regeneration
Y1  - 2018
ER  - 
TY  - GEN
A1  - Fuchs, Christiane
A1  - Weihs, Anna
A1  - Teuschl, Andreas
A1  - Hartinger, Joachim
A1  - Slezak, Paul
A1  - Mittermayr, Rainer
A1  - Redl, Heinz
A1  - Junger, Wolfgang
A1  - Sitte, Harald
A1  - Rünzler, Dominik
T1  - Shockwave Treatment Enhances Proliferation and Improves Wound Healing via Purinergic Signaling Linked ERK 1/2 Pathways
KW  - Shockwave treatment
KW  - Healing Processes
KW  - Signaling
Y1  - 2018
ER  - 
TY  - GEN
A1  - Fuchs, Christiane
A1  - Weihs, Anna
A1  - Teuschl, Andreas
A1  - Hartinger, Joachim
A1  - Slezak, Paul
A1  - Mittermayr, Rainer
A1  - Redl, Heinz
A1  - Junger, Wolfgang
A1  - Sitte, Harald
A1  - Rünzler, Dominik
T1  - Shockwave Treatment Augments Proliferation and Improves Wound Healing via Purinergic Signaling Linked ERK 1/2 Pathways
KW  - Shockwave treatment
KW  - Healing Processes
KW  - Signaling
Y1  - 2018
ER  - 
TY  - JOUR
A1  - Guillaume, Olivier
A1  - Park, Jaesung
A1  - Monforte Vila, Xavier
A1  - Gruber-Blum, Simone
A1  - Redl, Heinz
A1  - Petter-Puchner, Akexander
A1  - Teuschl, Andreas
T1  - Fabrication of silk mesh with enhanced cytocompatibility: preliminary in vitro investigation toward cell-based therapy for hernia repair
JF  - Journal of Materials Science: Materials in Medicine
KW  - Cytocompatibility
KW  - Hernia Repair
Y1  - 2018
ER  - 
TY  - GEN
A1  - Weihs, Anna
A1  - Fuchs, Christiane
A1  - Teuschl, Andreas
A1  - Hartinger, Joachim
A1  - Slezak, Paul
A1  - Mittermayr, Rainer
A1  - Redl, Heinz
A1  - Junger, Wolfgang
A1  - Sitte, Harald
A1  - Rünzler, Dominik
T1  - Shockwave treatment activates Erk1/2 pathways predominantly via P2Y receptor involvement
KW  - Shockwave
Y1  - 
ER  - 
TY  - JOUR
A1  - Teuschl, Andreas
A1  - Heimel, Patrick
A1  - Nürnberger, Sylvia
A1  - van Griensven, Martijn
A1  - Redl, Heinz
A1  - Nau, Thomas
T1  - A Novel Silk Fiber-Based Scaffold for Regeneration of the Anterior Cruciate Ligament: Histological Results From a Study in Sheep.
JF  - The American Journal of Sports Medicine
KW  - Ligament
KW  - Regeneration
Y1  - 2018
ER  - 
TY  - GEN
A1  - Teuschl, Andreas
A1  - Fuchs, Christiane
A1  - Feichtinger, Georg
A1  - Heher, Philipp
A1  - Heimel, Patrick
A1  - Schuh, Christina
A1  - Nürnberger, Sylvia
A1  - Nau, Thomas
A1  - Rünzler, Dominik
A1  - Redl, Heinz
T1  - Fibrin or Fibroin - Not only the "o" Makes the Difference
KW  - Fibrin
KW  - Fibroin
Y1  - 2018
ER  - 
TY  - JOUR
A1  - Teuschl, Andreas
A1  - Balmayor, Elizabeth
A1  - Redl, Heinz
A1  - van Griensven, Martijn
A1  - Dungel, Peter
T1  - Phototherapy With LED Light Modulates Healing Processes in an In Vitro Scratch Wound-Model Using 3 Different Cell Types
JF  - Dermatologic Surgery
KW  - Phototherapy
KW  - Healing Processes
Y1  - 
VL  - 41
IS  - 2
SP  - 261
EP  - 268
ER  - 
TY  - JOUR
A1  - Schuh, Christina
A1  - Hercher, David
A1  - Stainer, Michaela
A1  - Hopf, Rudolf
A1  - Teuschl, Andreas
A1  - Schmidhammer, Robert
A1  - Redl, Heinz
T1  - Extracorporeal shockwave treatment: A novel tool to improve Schwann cell isolation and culture
JF  - Cytotherapy
KW  - Extracorporeal shockwave
Y1  - 2018
ER  - 
TY  - GEN
A1  - Teuschl, Andreas
A1  - Nürnberger, Sylvia
A1  - Heimel, Patrick
A1  - Redl, Heinz
A1  - Nau, Thomas
T1  - Regeneration of the Anterior Cruciate Ligament Using a Silk-Fiber Based Scaffold - Histological Results
KW  - Tissue Regeneration
KW  - Ligaments
KW  - Scaffold
Y1  - 2018
ER  - 
TY  - JOUR
A1  - Nau, Thomas
A1  - Redl, Heinz
A1  - Teuschl, Andreas
T1  - Comment on: In Vivo Evaluation of Electrospun Polycaprolactone Graft for Anterior Cruciate Ligament Engineering
JF  - Tissue Engineering Part A
KW  - Grafting
KW  - Ligament
Y1  - 2018
ER  - 
TY  - JOUR
A1  - Gauillaume, Oliver
A1  - Teuschl, Andreas
A1  - Gruber-Blum, Simone
T1  - Emerging Trends in Abdominal Wall Reinforcement: Bringing Bio-Functionality to Meshes
JF  - Advanced Healthcare Materials
KW  - Abdominal Wall Reinforcement
Y1  - 
ER  - 
TY  - JOUR
A1  - Nau, Thomas
A1  - Teuschl, Andreas
T1  - Regeneration of the anterior cruciate ligament: Current strategies in tissue engineering
JF  - World Journal of Orthopedics
KW  - Ligament
KW  - Regeneration
KW  - Tissue Engineering
Y1  - 
VL  - 6
IS  - 1
ER  - 
TY  - JOUR
A1  - Weihs, Anna
A1  - Fuchs, Christiane
A1  - Teuschl, Andreas
A1  - Hartinger, Joachim
A1  - Slezak, Paul
A1  - Mittermayr, Rainer
A1  - Redl, Heinz
A1  - Junger, Wolfgang
A1  - Sitte, Harald
A1  - Rünzler, Dominik
T1  - Shock Wave Treatment Enhances Cell Proliferation and Improves Wound Healing by ATP Release-coupled Extracellular Signal-regulated Kinase (ERK) Activation
JF  - The Journal of biological chemistry
KW  - Shockwave
Y1  - 
ER  - 
TY  - JOUR
A1  - Schuh, Christina
A1  - Heher, Philipp
A1  - Weihs, Anna
A1  - Asmita, Banerjee
A1  - Wolbank, Susanne
A1  - Mittermayr, Rainer
A1  - Redl, Heinz
A1  - Rünzler, Dominik
A1  - Teuschl, Andreas
T1  - Adipose derived stem cells respond to in vitro extracorporeal shockwave treatment with increased stemness and multipotency
JF  - New Biotechnology
KW  - Shockwave
Y1  - 
ER  - 
TY  - GEN
A1  - Heher, Philipp
A1  - Fuchs, Christiane
A1  - Prüller, Johanna
A1  - Maleiner, Babette
A1  - Kollmitzer, Josef
A1  - Rünzler, Dominik
A1  - Teuschl, Andreas
A1  - Wolbank, Susanne
A1  - Redl, Heinz
T1  - A bioreactor-based 3D culture system for skeletal muscle engineering in fibrin scaffolds
KW  - Bioreactors
KW  - Cell Culture
KW  - Muscle
KW  - Scaffold
Y1  - 2018
ER  - 
TY  - JOUR
A1  - Banerjee, Asmita
A1  - Nürnberger, Sylvia
A1  - Hennerbichler, Simone
A1  - Riedl, Stefan
A1  - Schuh, Christina
A1  - Hacobian, Ara
A1  - Teuschl, Andreas
A1  - Eibl, Jürgen
A1  - Redl, Heinz
T1  - In toto differentiation of human amniotic membrane towards the Schwann cell lineage
JF  - 227-239
KW  - Membrane
KW  - In toto differentiation
Y1  - 2018
VL  - 15
IS  - 2
ER  - 
TY  - JOUR
A1  - Teuschl, Andreas
A1  - Neutsch, Lukas
A1  - Monforte Vila, Xavier
A1  - Rünzler, Dominik
A1  - van Griensven, Martijn
A1  - Gabor, Franz
A1  - Redl, Heinz
T1  - Enhanced cell adhesion on silk fibroin via lectin surface modification.
JF  - Acta Biomaterialia
KW  - Silk
KW  - Fibrin
Y1  - 
ER  - 
TY  - JOUR
A1  - Hohlrieder, Manfred
A1  - Teuschl, Andreas
A1  - Cicha, Klaus
A1  - van Griensven, Martijn
A1  - Redl, Heinz
A1  - Stampfl, Jürgen
T1  - Bioreactor and scaffold design for the mechanical stimulation of anterior cruciate ligament grafts
JF  - Biomedical materials and engineering
KW  - Bioreactors
KW  - Ligaments
KW  - Grafting
Y1  - 2018
VL  - 23
IS  - 3
SP  - 225
EP  - 237
ER  - 
TY  - JOUR
A1  - Dungel, Peter
A1  - Teuschl, Andreas
A1  - Banerjee, Asmita
A1  - Paier-Pourani, Jamile
A1  - Redl, Heinz
A1  - Kozlov, Andrey
T1  - Impact of mitochondria on nitrite metabolism in HL-1 cardiomyocytes
JF  - Frontiers in Physiology
KW  - Nitrite
KW  - Metabolism
Y1  - 2018
IS  - 4
ER  - 
TY  - JOUR
A1  - Teuschl, Andreas
A1  - Nürnberger, Sylvia
A1  - Redl, Heinz
A1  - Nau, Thomas
T1  - Articular cartilage tissue regeneration: current research strategies and outlook for the future
JF  - European Surgery
KW  - Tissue Regeneration
KW  - Cartilage Tissue
Y1  - 2018
VL  - 45
IS  - 3
SP  - 142
EP  - 153
ER  - 
TY  - JOUR
A1  - Teuschl, Andreas
A1  - van Griensven, Martijn
A1  - Redl, Heinz
T1  - Sericin removal from raw Bombys mori silk scaffolds of high hierarchical order
JF  - Tissue Eng Part C Methods
KW  - Scaffold
KW  - Sericin
Y1  - 2018
ER  - 
TY  - JOUR
A1  - Rieder, Bernhard
A1  - Weihs, Anna
A1  - Teuschl, Andreas
A1  - Knebl, Gerald
A1  - Kollmitzer, Josef
A1  - Redl, Heinz
A1  - Rünzler, Dominik
T1  - Evaluation of cell response on permanent and pulsed atmospheric pressure stressed cells
JF  - Journal of Tissue Engineering and Regenerative Medicine
KW  - Cells
KW  - Atmospheric Pressure
Y1  - 2018
VL  - 1
IS  - 6
SP  - 240
EP  - 240
ER  - 
TY  - JOUR
A1  - Teuschl, Andreas
A1  - Aigner, Elmar
A1  - Hohlrieder, Martin
A1  - Cicha, Klaus
A1  - Stampfl, Jürgen
A1  - Redl, Heinz
T1  - Stimulation of ligament tissue formation on a silk scaffold with mechanical loading using a custom-made bioreactor system
JF  - Journal of Tissue Engineering and Regenerative Medicine
KW  - Ligament
KW  - Tissue Formation
KW  - Scaffold
KW  - Silk
KW  - Bioreactor
Y1  - 2018
VL  - 1
IS  - 6
SP  - 51
EP  - 51
ER  - 
TY  - JOUR
A1  - Teuschl, Andreas
A1  - Ferguson, James
A1  - Szomolanyi, Pavol
A1  - Trattnig, Siegfried
A1  - Redl, Heinz
A1  - Nau, Thomas
T1  - Osteointegration of anterior cruciate ligament scaffolds fabricated of bombyx mori silk
JF  - Journal of Tissue Engineering and Regenerative Medicine
KW  - Osteointegration
KW  - Cruciate Ligament
KW  - Scaffold
KW  - Silk
Y1  - 2018
VL  - 1
IS  - 6
SP  - 181
EP  - 182
ER  - 
TY  - GEN
A1  - Hromada, Carina
A1  - Tomasch, Janine
A1  - Weihs, Anna
A1  - Rünzler, Dominik
A1  - Teuschl, Andreas
T1  - Engineering of 3D Tissue Constructs Using our Novel MagneTissue Bioreactor as Alternatives to Animal Models
KW  - Bioreactor
KW  - Biomaterials
Y1  - 
ER  - 
TY  - JOUR
A1  - Rieder, Bernhard
A1  - Weihs, Anna
A1  - Weidinger, Adelheid
A1  - Sczwarc, Dorota
A1  - Nürnberger, Sylvia
A1  - Redl, Heinz
A1  - Rünzler, Dominik
A1  - Huber-Gries, Carina
A1  - Teuschl, Andreas
T1  - Hydrostatic pressure-generated reactive oxygen species induce osteoarthritic conditions in cartilage pellet cultures
JF  - Scientific Reports
KW  - Bioreactor
KW  - Osteoarthritis
KW  - Cartilage
KW  - Reactive oxygen species
Y1  - 
ER  - 
TY  - JOUR
A1  - Teuschl, Andreas
A1  - Zipperle, Johannes
A1  - Huber-Gries, Carina
A1  - Kaplan, David
T1  - Silk fibroin based carrier system for delivery of fibrinogen and thrombin as coagulant supplements
JF  - Journal of Biomedical Materials Research
KW  - Fibrin
KW  - Silk
Y1  - 2018
VL  - 105
IS  - 3
ER  - 
TY  - JOUR
A1  - Simsa, Robin
A1  - Padma, Arvind
A1  - Heher, Philipp
A1  - Hellström, Mats
A1  - Teuschl, Andreas
A1  - Jenndahl, Lachmi
A1  - Bergh, Niklas
A1  - Fogelstrand, Per
T1  - Systematic in vitro comparison of decellularization protocols for blood vessels.
JF  - PLoS One
KW  - Tissue Engineering
KW  - Decellularization
KW  - Blood Vessel
Y1  - 
ER  - 
TY  - JOUR
A1  - Schneider, Karl Heinrich
A1  - Enayati, Marjan
A1  - Grasl, Christian
A1  - Walter, Ingrid
A1  - Budinsky, Lubos
A1  - Zebic, Gabriel
A1  - Kaun, Christoph
A1  - Wagner, Anja
A1  - Kratochwill, Klaus
A1  - Redl, Heinz
A1  - Teuschl, Andreas
A1  - Podesser, Bruno K.
A1  - Bergmeister, Helga
T1  - Acellular vascular matrix grafts from human placenta chorion: Impact of ECM preservation on graft characteristics, protein composition and in vivo performance.
JF  - Biomaterials
N2  - Small diameter vascular grafts from human placenta, decellularized with either Triton X-100 (Triton) or SDS and crosslinked with heparin were constructed and characterized. Graft biochemical properties, residual DNA, and protein composition were evaluated to compare the effect of the two detergents on graft matrix composition and structural alterations. Biocompatibility was tested in vitro by culturing the grafts with primary human macrophages and in vivo by subcutaneous implantation of graft conduits (n = 7 per group) into the flanks of nude rats. Subsequently, graft performance was evaluated using an aortic implantation model in Sprague Dawley rats (one month, n = 14). In situ graft imaging was performed using MRI angiography. Retrieved specimens were analyzed by electromyography, scanning electron microscopy, histology and immunohistochemistry to evaluate cell migration and the degree of functional tissue remodeling. Both decellularization methods resulted in grafts of excellent biocompatibility in vitro and in vivo, with low immunogenic potential. Proteomic data revealed removal of cytoplasmic proteins with relative enrichment of ECM proteins in decelluarized specimens of both groups. Noteworthy, LC-Mass Spectrometry analysis revealed that 16 proteins were exclusively preserved in Triton decellularized specimens in comparison to SDS-treated specimens. Aortic grafts showed high patency rates, no signs of thrombus formation, aneurysms or rupture. Conduits of both groups revealed tissue-specific cell migration indicative of functional remodeling. This study strongly suggests that decellularized allogenic grafts from the human placenta have the potential to be used as vascular replacement materials. Both detergents produced grafts with low residual immunogenicity and appropriate mechanical properties. Observed differences in graft characteristics due to preservation method had no impact on successful in vivo performance in the rodent model.
KW  - Biomaterial
KW  - Tissue Engineering
Y1  - 
SP  - 14
EP  - 26
ER  - 
TY  - JOUR
A1  - Teuschl, Andreas
A1  - Holnthoner, Wolfgang
A1  - Monforte, Xavier
T1  - Repopulation of an auricular cartilage scaffold, AuriScaff, perforated with an enzyme combination
JF  - Acta Biomater.
N2  - Biomaterials currently in use for articular cartilage regeneration do not mimic the composition or architecture of hyaline cartilage, leading to the formation of repair tissue with inferior characteristics. In this study we demonstrate the use of "AuriScaff", an enzymatically perforated bovine auricular cartilage scaffold, as a novel biomaterial for repopulation with regenerative cells and for the formation of high-quality hyaline cartilage. AuriScaff features a traversing channel network, generated by selective depletion of elastic fibers, enabling uniform repopulation with therapeutic cells. The complex collagen type II matrix is left intact, as observed by immunohistochemistry, SEM and TEM. The compressive modulus is diminished, but three times higher than in the clinically used collagen type I/III scaffold that served as control. Seeding tests with human articular chondrocytes (hAC) alone and in co-culture with human adipose-derived stromal/stem cells (ASC) confirmed that the network enabled cell migration throughout the scaffold. It also guides collagen alignment along the channels and, due to the generally traverse channel alignment, newly deposited cartilage matrix corresponds with the orientation of collagen within articular cartilage. In an osteochondral plug model, AuriScaff filled the complete defect with compact collagen type II matrix and enabled chondrogenic differentiation inside the channels. Using adult articular chondrocytes from bovine origin (bAC), filling of even deep defects with high-quality hyaline-like cartilage was achieved after 6 weeks in vivo. With its composition and spatial organization, AuriScaff provides an optimal chondrogenic environment for therapeutic cells to treat cartilage defects and is expected to improve long-term outcome by channel-guided repopulation followed by matrix deposition and alignment. STATEMENT OF SIGNIFICANCE: After two decades of tissue engineering for cartilage regeneration, there is still no optimal strategy available to overcome problems such as inconsistent clinical outcome, early and late graft failures. Especially large defects are dependent on biomaterials and their scaffolding, guiding and protective function. Considering the currently used biomaterials, structure and mechanical properties appear to be insufficient to fulfill this task. The novel scaffold developed within this study is the first approach enabling the use of dense cartilage matrix, repopulate it via channels and provide the cells with a compact collagen type II environment. Due to its density, it also provides better mechanical properties than materials currently used in clinics. We therefore think, that the auricular cartilage scaffold (AuriScaff) has a high potential to improve future cartilage regeneration approaches.
KW  - Auricular cartilage
KW  - Cartilage Regeneration
KW  - Human adipose derived stromal/stem cells
KW  - Tissue Engineering
KW  - Decellularization
Y1  - 2020
VL  - 2019
IS  - Mar/86
SP  - 207
EP  - 222
ER  - 
TY  - GEN
A1  - Schneider, Karl Heinrich
A1  - Aigner, Petra
A1  - Monforte Vila, Xavier
A1  - Holnthoner, Wolfgang
A1  - Teuschl, Andreas
A1  - Bergmeister, Helga
A1  - Redl, Heinz
T1  - Naturally derived acellular small diameter vascular grafts from human placenta for reconstructive surgery
KW  - Placenta
KW  - Grafting
KW  - Surgery
Y1  - 2018
ER  - 
TY  - JOUR
A1  - Schneider, Karl
A1  - Rohringer, Sabrina
A1  - Kapeller, Barbara
A1  - Grasl, Christian
A1  - Kiss, Herbert
A1  - Heber, Stefan
A1  - Walter, Ingrid
A1  - Teuschl, Andreas
A1  - Podesser, Bruno K.
A1  - Bergmeister, Helga
T1  - Riboflavin-mediated photooxidation to improve the characteristics of decellularized human arterial small diameter vascular grafts
JF  - Acta Biomater.
N2  - Vascular grafts with a diameter of less than 6 mm are made from a variety of materials and techniques to provide alternatives to autologous vascular grafts. Decellularized materials have been proposed as a possible approach to create extracellular matrix (ECM) vascular prostheses as they are naturally derived and inherently support various cell functions. However, these desirable graft characteristics may be limited by alterations of the ECM during the decellularization process leading to decreased biomechanical properties and hemocompatibility. In this study, arteries from the human placenta chorion were decellularized using two distinct detergents (Triton X-100 or SDS), which differently affect ECM ultrastructure. To overcome biomechanical strength loss and collagen fiber exposure after decellularization, riboflavin-mediated UV (RUV) crosslinking was used to uniformly crosslink the collagenous ECM of the grafts. Graft characteristics and biocompatibility with and without RUV crosslinking were studied in vitro and in vivo. RUV-crosslinked ECM grafts showed significantly improved mechanical strength and smoothening of the luminal graft surfaces. Cell seeding using human endothelial cells revealed no cytotoxic effects of the RUV treatment. Short-term aortic implants in rats showed cell migration and differentiation of host cells. Functional graft remodeling was evident in all grafts. Thus, RUV crosslinking is a preferable tool to improve graft characteristics of decellularized matrix conduits.
KW  - Tissue Engineering
KW  - Biomaterials
KW  - Vascularization
Y1  - 2021
VL  - 2020
IS  - 116
SP  - 246
EP  - 258
ER  - 
TY  - JOUR
A1  - Nürnberger, Sylvia
A1  - Schneider, Cornelia
A1  - van Osch, Gerjo
A1  - Keibl, Claudia
A1  - Rieder, Bernhard
A1  - Monforte, Xavier
A1  - Teuschl, Andreas
A1  - Mühleder, Severin
A1  - Holnthoner, Wolfgang
A1  - Schädl, Barbara
A1  - Gahleitner, Christoph
A1  - Redl, Heinz
A1  - Wolbank, Susanne
T1  - Repopulation of an auricular cartilage scaffold, AuriScaff, perforated with an enzyme combination.
JF  - Acta Biomaterialia
KW  - Tissue Engineering
KW  - Decellularization
KW  - Cartilage
Y1  - 
ER  - 
TY  - JOUR
A1  - Schneider, Karl H.
A1  - Rohringer, Sabrina
A1  - Kapeller, Barbara
A1  - Grasl, Christian
A1  - Kiss, Herbert
A1  - Heber, Stefan
A1  - Walter, Ingrid
A1  - Teuschl, Andreas H.
A1  - Podesser, Bruno K.
A1  - Bergmeister, Helga
T1  - Riboflavin-mediated photooxidation to improve the characteristics of decellularized human arterial small diameter vascular grafts
JF  - Acta Biomaterialia
N2  - Vascular grafts with a diameter of less than 6 mm are made from a variety of materials and techniques to provide alternatives to autologous vascular grafts. Decellularized materials have been proposed as a possible approach to create extracellular matrix (ECM) vascular prostheses as they are naturally derived and inherently support various cell functions. However, these desirable graft characteristics may be limited by alterations of the ECM during the decellularization process leading to decreased biomechanical properties and hemocompatibility. In this study, arteries from the human placenta chorion were decellularized using two distinct detergents (Triton X-100 or SDS), which differently affect ECM ultrastructure. To overcome biomechanical strength loss and collagen fiber exposure after decellularization, riboflavin-mediated UV (RUV) crosslinking was used to uniformly crosslink the collagenous ECM of the grafts. Graft characteristics and biocompatibility with and without RUV crosslinking were studied in vitro and in vivo. RUV-crosslinked ECM grafts showed significantly improved mechanical strength and smoothening of the luminal graft surfaces. Cell seeding using human endothelial cells revealed no cytotoxic effects of the RUV treatment. Short-term aortic implants in rats showed cell migration and differentiation of host cells. Functional graft remodeling was evident in all grafts. Thus, RUV crosslinking is a preferable tool to improve graft characteristics of decellularized matrix conduits.
KW  - Tissue Engineering
KW  - Small diameter vascular graft
KW  - Biomaterials
KW  - Decellularization
KW  - Surface modification
Y1  - 
VL  - 116
SP  - 246
EP  - 258
ER  -