TY  - JOUR
A1  - Schuh, Christina
A1  - Heher, Philipp
A1  - Weihs, Anna
A1  - Fuchs, Christiane
A1  - Gabriel, Christian
A1  - Wolbank, Susanne
A1  - Mittermayr, Rainer
A1  - Redl, Heinz
A1  - Rünzler, Dominik
A1  - Teuschl, Andreas
T1  - In vitro extracorporeal shock wave treatment enhances stemness and preserves multipotency of rat and human adipose-derived stem cells
JF  - Journal of Cytotherapy
KW  - Shockwave
Y1  - 
ER  - 
TY  - JOUR
A1  - Simböck, Elisabeth
A1  - Marksteiner, Jessica
A1  - Machacek, Thomas
A1  - Wiessner, Katharina
A1  - Gepp, Barbara
A1  - Jesenberger, Veronika
A1  - Weihs, Anna
A1  - Leitner, Rita
T1  - The Power of Problem Based Learning beyond its Didactic Attributes
JF  - Journal of Problem Based Learning in Higher Education (JPBLHE)
N2  - Hybrid courses with a focus on practice-orientated education and self-guided learning phases are on the rise on the higher education sector. Disciplines in Life Sciences implicate a high degree of practical laboratory expertise. The University of Applied Sciences (UAS) in Vienna, Austria, has thus been endeavoured offering students a high qualitative education integrating hybrid courses based on PBL principles, which consist of on-site (including the transmission of necessary background and practical laboratory training) and off-site (including self-study phases) sessions. As practical laboratory units are central in those courses, the restrictive measures, including the transition to a complete online teaching format due to the first Covid-19-pandemic lock-down, had severe effects on the implementation and the quality of the curriculum. According to surveys made specifically to address this problematic situation, it can be concluded that on-site practical units are fundamental for certain disciplines such as Life Sciences.
KW  - Problem-based Learning
KW  - Life Science didactics
KW  - Hybrid PBL-methods
KW  - COVID-19
KW  - Life Science Education
Y1  - 
VL  - 9
IS  - 1
SP  - 109
EP  - 130
ER  - 
TY  - JOUR
A1  - Quartinello, Felice
A1  - Tallian, Claudia
A1  - Auer, Julia
A1  - Schön, Herta
A1  - Vielnascher, Robert
A1  - Weinberger, Simone
A1  - Wieland, Karin
A1  - Weihs, Anna
A1  - Rollett, Alexandra
A1  - Lendl, Bernhard
A1  - Teuschl, Andreas
A1  - Pellis, Alessandro
A1  - Gübitz, Georg
T1  - Smart Textiles in Wound Care: Functionalization of Cotton/PET Blends with Antimicrobial Nanocapsules
JF  - Journal of Materials Chemistry B
KW  - Smart textiles
KW  - Tissue Engineering
KW  - Regenerative Medicine
KW  - Antimicrobial
KW  - Wound
Y1  - 2019
ER  - 
TY  - JOUR
A1  - Tallian, Claudia
A1  - Herrero-Rollett, Alexandra
A1  - Stadler, Karina
A1  - Vielnascher, Robert
A1  - Wieland, Karin
A1  - Weihs, Anna
A1  - Pellis, Alessandro
A1  - Teuschl, Andreas
A1  - Lendl, Bernhard
A1  - Amenitsch, Heinz
A1  - Guebitz, Georg M.
T1  - Structural insights into pH-responsive drug release of self-assembling human serum albumin-silk fibroin nanocapsules.
JF  - European Journal of Pharmaceutics and Biopharmaceutics
N2  - Inflammation processes are associated with significant decreases in tissue or lysosomal pH from 7.4 to 4, a fact that argues for the application of pH-responsive drug delivery systems. However, for their design and optimization a full understanding of the release mechanism is crucial. In this study we investigated the pH-depending drug release mechanism and the influence of silk fibroin (SF) concentration and SF degradation degree of human serum albumin (HSA)-SF nanocapsules. Sonochemically produced nanocapsules were investigated regarding particle size, colloidal stability, protein encapsulation, thermal stability and drug loading properties. Particles of the monodisperse phase showed average hydrodynamic radii between 438 and 888 nm as measured by DLS and AFM and a zeta potential of -11.12 ± 3.27 mV. Together with DSC results this indicated the successful production of stable nanocapsules. ATR-FTIR analysis demonstrated that SF had a positive effect on particle formation and stability due to induced beta-sheet formation and enhanced crosslinking. The pH-responsive release was found to depend on the SF concentration. In in-vitro release studies, HSA-SF nanocapsules composed of 50% SF showed an increased pH-responsive release for all tested model substances (Rhodamine B, Crystal Violet and Evans Blue) and methotrexate at the lowered pH of 4.5 to pH 5.4, while HSA capsules without SF did not show any pH-responsive drug release. Mechanistic studies using confocal laser scanning microscopy (CLSM) and small angle X-ray scattering (SAXS) analyses showed that increases in particle porosity and decreases in particle densities are directly linked to pH-responsive release properties. Therefore, the pH-responsive release mechanism was identified as diffusion controlled in a novel and unique approach by linking scattering results with in vitro studies. Finally, cytotoxicity studies using the human monocytic THP-1 cell line indicated non-toxic behavior of the drug loaded nanocapsules when applied in a concentration of 62.5 µg mL-1.
KW  - Biomaterial
KW  - Tissue Engineering
Y1  - 
ER  - 
TY  - GEN
A1  - Teuschl, Andreas
A1  - Schuh, Christina
A1  - Weihs, Anna
A1  - Guillaume, Olivier
A1  - Monforte Vila, Xavier
A1  - Redl, Heinz
A1  - Kaplan, David
A1  - Rünzler, Dominik
T1  - Tailoring bioactivity of silk-based biomaterials via delivering and functionalization strategies with fibrinogen/thrombin, plant lectins or laminin
KW  - Biomaterials
KW  - Tissue Engineering
KW  - Silk
Y1  - 
ER  - 
TY  - GEN
A1  - Salzer, Elias
A1  - Rieder, Bernhard
A1  - Monforte Vila, Xavier
A1  - Weihs, Anna
A1  - Rünzler, Dominik
A1  - Teuschl, Andreas
T1  - Evaluation of a novel hydrostatic pressure bioreactor on bovine cartilage chips
KW  - Bioreactor
KW  - Tissue Engineering
KW  - Cartilage
Y1  - 
ER  - 
TY  - GEN
A1  - Slezak, Paul
A1  - Rose, Roland
A1  - Hercher, David
A1  - Weihs, Anna
A1  - Fuchs, Christiane
A1  - Redl, Heinz
A1  - Mittermayr, Rainer
A1  - Slezak, Cyrill
T1  - Tracking therapeutic shockwaves and their impact on regeneration
KW  - Shockwave Therapy
KW  - Tissue Regeneration
Y1  - 
ER  - 
TY  - CHAP
A1  - Fuchs, Christiane
A1  - Weihs, Anna
A1  - Szwarc, Dorota
A1  - Mittermayr, Rainer
A1  - Rünzler, Dominik
A1  - Teuschl, Andreas
T1  - Shock wave treatment of muscle (stem) cells - a new implementation for regeneration
T2  - Proceedings of the 20th International Congress of the ISMST
KW  - Shockwave treatment
KW  - Muscle Cells
KW  - Regeneration
Y1  - 2018
ER  - 
TY  - GEN
A1  - Fuchs, Christiane
A1  - Weihs, Anna
A1  - Szwarc, Dorota
A1  - Mittermayr, Rainer
A1  - Rünzler, Dominik
A1  - Teuschl, Andreas
T1  - Shock wave treatment of muscle (stem) cells - a new implementation for regeneration
KW  - Shockwave treatment
KW  - Muscle Cells
KW  - Regeneration
Y1  - 2018
ER  - 
TY  - GEN
A1  - Teuschl, Andreas
A1  - Weihs, Anna
A1  - Fuchs, Christiane
A1  - Monforte Vila, Xavier
T1  - Silk as a versatile biomaterial for musculoskeletal tissue engineering
KW  - Silk
KW  - Biomaterials
Y1  - 2018
ER  - 
TY  - GEN
A1  - Szwarc, Dorota
A1  - Fuchs, Christiane
A1  - Weihs, Anna
A1  - Rünzler, Dominik
T1  - Molecular mechanisms underlying the potential of shock wave treatment for cardiac therapy
KW  - Shockwave treatment
KW  - Cardiac
Y1  - 2018
ER  - 
TY  - GEN
A1  - Szwarc, Dorota
A1  - Fuchs, Christiane
A1  - Weihs, Anna
A1  - Monforte Vila, Xavier
A1  - Hanetseder, Dominik
A1  - Teuschl, Andreas
A1  - Rünzler, Dominik
T1  - The effect of shock waves on in vitro cartilage development in silk scaffolds
KW  - Shockwave treatment
KW  - In Vitro
KW  - Cartilage
KW  - Silk
Y1  - 2018
ER  - 
TY  - GEN
A1  - Teuschl, Andreas
A1  - Fuchs, Christiane
A1  - Weihs, Anna
A1  - Heimel, Patrick
A1  - Rünzler, Dominik
A1  - Redl, Heinz
A1  - Nau, Thomas
T1  - The Silk Road from Textiles to Novel Medical Implants
KW  - Silk
Y1  - 2018
ER  - 
TY  - CHAP
A1  - Fuchs, Christiane
A1  - Szwarc, Dorota
A1  - Weihs, Anna
A1  - Rünzler, Dominik
T1  - Shock wave treatment of 3D cardiac model systems activates ERK 1/2 signaling pathway and influences cardiomyogenesis
T2  - Proceedings of PACT "Designer Cells go Clinical" Symposium
KW  - Shockwave treatment
KW  - Cardiac
Y1  - 2018
ER  - 
TY  - CHAP
A1  - Fuchs, Christiane
A1  - Szwarc, Dorota
A1  - Weihs, Anna
A1  - Rünzler, Dominik
T1  - Shock wave treatment of 3D cardiac model systems activates ERK 1/2 signaling pathway and influences cardiomyogenesis
T2  - Proceedings of LBG Meeting for Health Sciences 2016
KW  - Shockwave treatment
KW  - Cardiac
Y1  - 2018
ER  - 
TY  - GEN
A1  - Fuchs, Christiane
A1  - Szwarc, Dorota
A1  - Weihs, Anna
A1  - Rünzler, Dominik
T1  - Shock wave treatment of 3D cardiac model systems activates ERK 1/2 signaling pathway and influences cardiomyogenesis
KW  - Shockwave treatment
KW  - Cardiac
Y1  - 2018
ER  - 
TY  - CHAP
A1  - Fuchs, Christiane
A1  - Szwarc, Dorota
A1  - Weihs, Anna
A1  - Rünzler, Dominik
T1  - Shock wave treatment positively influences cardiomyogenesis in an energy-dependent manner
T2  - Proceedings of PACT "Designer Cells go Clinical" Symposium
KW  - Shockwave treatment
KW  - Cardiac
Y1  - 2018
ER  - 
TY  - GEN
A1  - Weihs, Anna
T1  - Shock wave treatment for in vitro tissue engineering applications
KW  - Shockwave treatment
KW  - In Vitro
KW  - Tissue Engineering
Y1  - 2018
ER  - 
TY  - GEN
A1  - Weihs, Anna
T1  - Shock wave treatment for in vitro tissue engineering applications
KW  - Shockwave treatment
KW  - In Vitro
KW  - Tissue Engineering
Y1  - 2018
ER  - 
TY  - GEN
A1  - Weihs, Anna
T1  - Mechanotransduction in shock wave treatment
KW  - Shockwave treatment
KW  - Mechanotransduction
Y1  - 2018
ER  - 
TY  - GEN
A1  - Fuchs, Christiane
A1  - Weihs, Anna
A1  - Teuschl, Andreas
A1  - Hartinger, Joachim
A1  - Slezak, Paul
A1  - Mittermayr, Rainer
A1  - Redl, Heinz
A1  - Junger, Wolfgang
A1  - Sitte, Harald
A1  - Rünzler, Dominik
T1  - Shockwave Treatment Enhances Proliferation and Improves Wound Healing via Purinergic Signaling Linked ERK 1/2 Pathways
KW  - Shockwave treatment
KW  - Healing Processes
KW  - Signaling
Y1  - 2018
ER  - 
TY  - GEN
A1  - Fuchs, Christiane
A1  - Weihs, Anna
A1  - Teuschl, Andreas
A1  - Hartinger, Joachim
A1  - Slezak, Paul
A1  - Mittermayr, Rainer
A1  - Redl, Heinz
A1  - Junger, Wolfgang
A1  - Sitte, Harald
A1  - Rünzler, Dominik
T1  - Shockwave Treatment Augments Proliferation and Improves Wound Healing via Purinergic Signaling Linked ERK 1/2 Pathways
KW  - Shockwave treatment
KW  - Healing Processes
KW  - Signaling
Y1  - 2018
ER  - 
TY  - GEN
A1  - Weihs, Anna
A1  - Fuchs, Christiane
A1  - Teuschl, Andreas
A1  - Hartinger, Joachim
A1  - Slezak, Paul
A1  - Mittermayr, Rainer
A1  - Redl, Heinz
A1  - Junger, Wolfgang
A1  - Sitte, Harald
A1  - Rünzler, Dominik
T1  - Shockwave treatment activates Erk1/2 pathways predominantly via P2Y receptor involvement
KW  - Shockwave
Y1  - 
ER  - 
TY  - THES
A1  - Weihs, Anna
T1  - Elucidation of extracorporeal shock wave treatment triggered intracellular processes
N2  - Die Wirkung von Stoßwellen auf den menschlichen Körper ist bereits seit Jahrzehnten bekannt. Ihr zerstörerisches Potential wird seit den 1980-ern zur Behandlung und Desintegration von Nierensteinen angewendet, wo die Stoßwellentherapie heutzutage die Standardbehandlung darstellt. Der erste unerwartete Effekt der Therapie - die Verdichtung des Beckenkammes - wurde schon damals bei Folgeuntersuchungen von Patienten mit Nierensteinen festgestellt. Die Beobachtung, dass Stoßwellen Effekte auf Knochen hervorrufen, ebnete den Weg für die Anwendung der Stoßwellentherapie in Bereichen abseits der Urologie.
Mittlerweile wird die Stoßwellentherapie nicht nur zur Behandlung von Knochenbruchheilungsstörungen und Tendinopathien, sondern auch bei Weichteilwundheilungsstörungen eingesetzt. Trotz der vielfältigen Anwendungsgebiete ist der zugrunde liegende Wirkungsmechanismus des positiven Effekts der Stoßwellentherapie bis heute noch nicht vollständig aufgeklärt. Die Aktivierung von mechanotransduktiven Signalwegen wurde bereits teilweise in vitro und in vivo gezeigt, jedoch meist bei knöchernen Indikationen. Doch auch für die Anwendung der Stoßwellentherapie in der (verzögerten) Wundheilung ist die Aufklärung des Wirkungsmechanismus essentiell. Erst dadurch könnte diese nicht- invasive, effiziente und großteils Nebenwirkungs-freie Therapieform auch in diesem Bereich als mögliche Standardtherapieform genutzt werden. In dieser Dissertation wurde zuerst ein Set-up zur in vitro Stoßwellenanwendung optimiert. Mithilfe eines Molekül-Aufnahme Assays wurden die technischen Parameter für die in vitro Stoßwellenbehandlung festgelegt. Mit diesem in vitro Aufbau sollten daraufhin jene intrazellulären Mechanismen identifiziert werden, die von der Stoßwelle beeinflusst werden. In einem in vivo Modell sollte schlussendlich die Rolle dieser Mechanismen am wundheilungsfördernden Effekt der Stoßwelle geklärt werden. Um möglichst universelle Effekte der Stoßwelle auf intrazelluläre Signalwege zu untersuchen, wurden verschiedenste Zelllinien verwendet. Diese beinhalteten die humane U937 Monozyten Zelllinie, humane Jurkat T-Zellen, die humane MG63 Osteosarcoma Zelllinie, die murine C3H10T1/2 mesenchymale Progenitor Zelllinie sowie primäre humane mononukleäre Zellen des peripheren Blutes. Die in den proliferativen Effekt der Stowellentherapie involvierte Signalkaskade konnte darauffolgend erstmals sowohl in murinen C3H10T1/2 Zellen als auch in humanen Fettstammzellen (adipose tissue-derived stem cells) und in humanen Jurkat T-Zellen detailliert beschrieben werden. Außerdem wurde ATP als entscheidendes Signalmolekül identifiziert, welches nach Freisetzung durch Stoßwellenbehandlung mittels purinergem Signaling die Erk1/2 Signalkaskade aktiviert. Für die dadurch gesteigerte Proliferation in stoßwellenbehandelten Zellen ist die Erk1/2 Aktivierung essentiell. In einem in vivo Model für gestörte Wundheilung in der Ratte wurde die Hypothese zur entscheidenden Rolle des Erk1/2 Signalweges im wundheilungsfördernden Effekt der Stoßwelle bestätigt. In dieser Dissertation wurde gezeigt, dass die durch purinerges Signaling aktivierte Erk1/2 Signalkaskade eine entscheidende Rolle in der durch Stoßwellentherapie beschleunigten Zellproliferation in vitro und Wundheilung in vivo übernimmt. Das damit erweiterte Verständnis der Wirkungsmechanismen der Stoßwellentherapie kann zur Weiterentwicklung dieser Behandlungsform als zukünftige Standardtherapie bei Wundheilungsstörungen, wie z.B. für diabetische oder chronische Wunden, beitragen.
N2  - Since the early 1980s, shock wave treatment has been the golden standard treatment option for the disintegration of kidney stones in urology. A wide range of beneficial effects of shock waves on the human body was soon identified, starting with first observations of bone densification at the iliac crest after treatment of kidney stones. Since then, the indications for shock wave therapy have conquered areas apart from the field of urology. Nowadays, shock wave therapy is used for a variety of indications such as tendinopathies or impaired bone healing.
Furthermore, patients suffering from poor wound healing such as diabetic foot ulcers and also chronic, non-healing wounds are treated successfully with shock waves. Despite the versatile application fields of shock wave therapy, the general principles underlying the beneficial effect of this treatment still remain to be fully elucidated. Several in vitro and in vivo studies, mostly involving osteoblast like cells and the osteo-inductive potential of shock wave treatment, already highlighted the role of the activation of mechanotransductory signaling pathways. For the clinical application of shock wave therapy as an accepted treatment for critically healing wounds (e.g. chronic or diabetic wounds, burns), general mechanistic evidence to explain the underlying mechanisms is essential. These data would facilitate the standardized application of this non-invasive, cost efficient and low- risk bearing therapy, which can be performed in an outpatient setting.
First of all, an in vitro set-up was established and the necessary technical parameters for the optimal application of shock wave treatment on cell cultures were defined in this thesis. For this purpose, a molecule uptake assay was used as a functional assay. The following aims of this study were to elucidate the effect of shock wave treatment on intracellular signaling in vitro and to ultimately describe their role in the wound healing effect of shock wave treatment in vivo. To identify universal effects of shock wave treatment on intracellular signaling mechanisms, various cell lines were used, including the human U937 monocytic cell line, a human Jurkat T-cell line, the human MG63 osteosarcoma cell line, the C3H10T1/2 mouse mesenchymal progenitor cell line as well as primary human peripheral mononuclear cells. For the first time, the affected signaling cascade leading to the proliferative effect of shock wave treatment in vitro was described in detail in mouse C3H10T1/2 cells as well as in human adipose tissue-derived stem cells and human Jurkat T-cells. Further, ATP release from shock wave treated cells was shown to initiate intracellular Erk1/2 signaling activation via purinergic signaling. The thereby ultimately increased proliferation was reported to be dependent on shock wave treatment triggered Erk1/2 pathway activation. An in vivo study on impaired wound healing in rats confirmed the hypothesis on the essential role of Erk1/2 signaling in the shock wave treatment induced wound healing effect. Data clearly indicate the crucial importance of the Erk1/2 signaling cascade in the proliferative and wound healing effect of shock wave treatment.
Conclusively, purinergic signaling activated Erk1/2 signaling cascades play an essential role in the shock wave treatment induced proliferative and wound healing effect. The thereby broadened knowledge on the underlying mechanistic principles of the effect of shock wave treatment contributes to the establishment of shock wave therapy as a feasible standard treatment for soft tissue wound healing disorders such as diabetic or chronic wounds.
KW  - Healing
KW  - Shockwave
Y1  - 2018
ER  - 
TY  - GEN
A1  - Weihs, Anna
T1  - Aufklärung des Wirkungsmechanismus der Stoßwellentherapie in der Wundheilung
KW  - Shockwave
Y1  - 
ER  - 
TY  - JOUR
A1  - Weihs, Anna
A1  - Fuchs, Christiane
A1  - Teuschl, Andreas
A1  - Hartinger, Joachim
A1  - Slezak, Paul
A1  - Mittermayr, Rainer
A1  - Redl, Heinz
A1  - Junger, Wolfgang
A1  - Sitte, Harald
A1  - Rünzler, Dominik
T1  - Shock Wave Treatment Enhances Cell Proliferation and Improves Wound Healing by ATP Release-coupled Extracellular Signal-regulated Kinase (ERK) Activation
JF  - The Journal of biological chemistry
KW  - Shockwave
Y1  - 
ER  - 
TY  - JOUR
A1  - Schuh, Christina
A1  - Heher, Philipp
A1  - Weihs, Anna
A1  - Asmita, Banerjee
A1  - Wolbank, Susanne
A1  - Mittermayr, Rainer
A1  - Redl, Heinz
A1  - Rünzler, Dominik
A1  - Teuschl, Andreas
T1  - Adipose derived stem cells respond to in vitro extracorporeal shockwave treatment with increased stemness and multipotency
JF  - New Biotechnology
KW  - Shockwave
Y1  - 
ER  - 
TY  - GEN
A1  - Weihs, Anna
T1  - Revealing underlying mechanisms of shock wave treatment induced wound healing
KW  - Shockwave
Y1  - 
ER  - 
TY  - JOUR
A1  - Rieder, Bernhard
A1  - Weihs, Anna
A1  - Teuschl, Andreas
A1  - Knebl, Gerald
A1  - Kollmitzer, Josef
A1  - Redl, Heinz
A1  - Rünzler, Dominik
T1  - Evaluation of cell response on permanent and pulsed atmospheric pressure stressed cells
JF  - Journal of Tissue Engineering and Regenerative Medicine
KW  - Cells
KW  - Atmospheric Pressure
Y1  - 2018
VL  - 1
IS  - 6
SP  - 240
EP  - 240
ER  - 
TY  - JOUR
A1  - Weihs, Anna
A1  - Junger, Wolfgang
A1  - Schaden, Wolfgang
A1  - Sitte, Harald
A1  - Rünzler, Dominik
T1  - Extracorporeal shockwave treatment induced extracellular ATP release - a potential mechanism to activate wound healing
JF  - Journal of Tissue Engineering and Regenerative Medicine
KW  - Shockwave treatment
KW  - Healing Processes
Y1  - 2018
VL  - 1
IS  - 6
SP  - 381
EP  - 381
ER  - 
TY  - JOUR
A1  - Rünzler, Dominik
A1  - Weihs, Anna
A1  - Junger, Wolfgang
A1  - Sitte, Harald
A1  - Schaden, Wolfgang
T1  - Extracorporeal shock waves - a novel method of mechanostimulation in tissue regeneration
JF  - Journal of Tissue Engineering and Regenerative Medicine
KW  - Shockwave treatment
KW  - Mechanostimulation
Y1  - 2018
VL  - 1
IS  - 6
SP  - 380
EP  - 380
ER  - 
TY  - GEN
A1  - Weihs, Anna
A1  - Junger, Wolgang G.
A1  - Schaden, Wolfgang
A1  - Sitte, Harald H.
A1  - Rünzler, Dominik
T1  - Extracellular ATP release - a potential benefit in shock wave treatment
KW  - Shockwave
Y1  - 2019
ER  - 
TY  - CHAP
A1  - Weihs, Anna
A1  - Knebl, Gerald
A1  - Redl, Heinz
A1  - Rünzler, Dominik
T1  - Evaluation of cell migration methods in 3D hydrogels for tissue engineering applications
T2  - 3. Forschungsforum der österreichischen Fachhochschulen / Fachhochschule Kärnten
KW  - Cells
Y1  - 2019
SN  - 978-3-853912850
SP  - 490
EP  - 491
ER  - 
TY  - GEN
A1  - Weihs, Anna
T1  - Establishment of an in vitro experimental set-up to evaluate the biological effects of extracorporeal shock wave treatment
KW  - Shockwave
Y1  - 2019
ER  - 
TY  - CHAP
A1  - Knebl, Gerald
A1  - Weihs, Anna
A1  - Weingant, Michaela
A1  - Steininger, Thomas
A1  - Redl, Heinz
A1  - Rünzler, Dominik
T1  - Automatisierte Datenauswertung eines Boyden-Mikro-Chemotaxis-Kammer Zell-Migrations Assays.
T2  - 2. Forschungsforum der österreichischen Fachhochschulen
KW  - Cells
KW  - Data Analysis
Y1  - 2019
SN  - 978-3-8322-7023-0
SP  - 412
EP  - 417
ER  - 
TY  - GEN
A1  - Hromada, Carina
A1  - Tomasch, Janine
A1  - Weihs, Anna
A1  - Rünzler, Dominik
A1  - Teuschl, Andreas
T1  - Engineering of 3D Tissue Constructs Using our Novel MagneTissue Bioreactor as Alternatives to Animal Models
KW  - Bioreactor
KW  - Biomaterials
Y1  - 
ER  - 
TY  - JOUR
A1  - Rieder, Bernhard
A1  - Weihs, Anna
A1  - Weidinger, Adelheid
A1  - Sczwarc, Dorota
A1  - Nürnberger, Sylvia
A1  - Redl, Heinz
A1  - Rünzler, Dominik
A1  - Huber-Gries, Carina
A1  - Teuschl, Andreas
T1  - Hydrostatic pressure-generated reactive oxygen species induce osteoarthritic conditions in cartilage pellet cultures
JF  - Scientific Reports
KW  - Bioreactor
KW  - Osteoarthritis
KW  - Cartilage
KW  - Reactive oxygen species
Y1  - 
ER  - 
TY  - JOUR
A1  - Johannes, Hackethal
A1  - Weihs, Anna
A1  - Karner, Lisa
A1  - Metzger, Magdalena
A1  - Dungel, Peter
A1  - Hennerbichler, Simone
A1  - Redl, Heinz
A1  - Teuschl-Woller, Andreas Herbert
T1  - Novel Human Placenta-Based Extract for Vascularization Strategies in Tissue Engineering
JF  - Tissue Eng Part C Methods
N2  - There is critical unmet need for new vascularized tissues to support or replace injured tissues and organs. Various synthetic and natural materials were already established for use of two-dimensional (2D) and three-dimensional (3D) in vitro neovascularization assays, however, they still cannot mimic the complex functions of the sum of the extracellular matrix (ECM) in native intact tissue. Currently, this issue is only addressed by artificial products such as Matrigel™, which comprises a complex mixture of ECM proteins, extracted from animal tumor tissue. Despite its outstanding bioactivity, the isolation from tumor tissue hinders its translation into clinical applications. Since nonhuman ECM proteins may cause immune reactions, as are frequently observed in clinical trials, human ECM proteins represent the best option when aiming for clinical applications. Here, we describe an effective method of isolating a human placenta substrate (hpS) that induces the spontaneous formation of an interconnected network of green fluorescence-labeled human umbilical vein endothelial cells (gfpHUVECs) in vitro. The substrate was biochemically characterized by using a combination of bicinchoninic acid (BCA) assay, DNA, and glycosaminoglycan (GAG) content assays, sodium dodecyl sulfate/polyacrylamide gel electrophoresis (SDS-PAGE) analysis and Western blot, angiogenesis arrays, chromatographic thrombin detection, high performance liquid chromatography (HPLC)-based amino acid quantification analysis, and assessment of antimicrobial properties. 2D in vitro cell culture experiments have been performed to determine the vasculogenic potential of hpS, which demonstrated that cell networks developed on hpS show a significantly higher degree of complexity (number of tubules/junctions; total/mean tube length) when compared with Matrigel. As 3D cell culture techniques represent a more accurate representation of the in vivo condition, the substrate was 3D solidified using various natural polymers. 3D in vitro vasculogenesis assays have been performed by seeding gfpHUVECs in an hpS-fibrinogen clot. In conclusion, hpS provides a potent human/material-based alternative to xenogenic-material-based biomaterials for vascularization strategies in tissue engineering.
KW  - Tissue Engineering
KW  - Biomaterials
KW  - HUVEC
KW  - Acellular biological matrices
KW  - Angiogenesis and vasculogenesis
Y1  - 
VL  - 27
IS  - 11
SP  - 616
EP  - 632
ER  - 
TY  - JOUR
A1  - Rohringer, Sabrina
A1  - Holnthoner, Wolfgang
A1  - Hackl, Matthias
A1  - Weihs, Anna
A1  - Rünzler, Dominik
A1  - Skalicky, Susanna
A1  - Karbiener, Michael
A1  - Scheideler, Marcel
A1  - Pröll, Johannes
A1  - Gabriel, Christian
A1  - Schweighofer, Bernhard
A1  - Gröger, Marion
A1  - Spittler, Andreas
A1  - Grillari, Johannes
A1  - Redl, Heinz
T1  - Molecular and cellular effects of in vitro shockwave treatment on lymphatic endothelial cells.
JF  - PLoS one
KW  - Shockwave
Y1  - 
ER  - 
TY  - JOUR
A1  - Gollmann-Tepeköylü, Can
A1  - Graber, Michael
A1  - Hirsch, Jakob
A1  - Mair, Sophia
A1  - Naschberger, Andreas
A1  - Pölzl, Leo
A1  - Nägele, Felix
A1  - Kirchmair, Elke
A1  - Degenhart, Gerald
A1  - Demetz, Egon
A1  - Hilbe, Richard
A1  - Chen, Hao-Yu
A1  - Engert, James C
A1  - Böhm, Anna
A1  - Franz, Nadja
A1  - Lobenwein, Daniela
A1  - Lener, Daniela
A1  - Fuchs, Christiane
A1  - Weihs, Anna
A1  - Töchterle, Sonja
A1  - Vogel, Georg F
A1  - Schweiger, Victor
A1  - Eder, Jonas
A1  - Pietschmann, Peter
A1  - Seifert, Markus
A1  - Kronenberg, Florian
A1  - Coassin, Stefan
A1  - Blumer, Michael
A1  - Hackl, Hubert
A1  - Meyer, Dirk
A1  - Feuchtner, Gudrun
A1  - Kirchmair, Rudolf
A1  - Troppmair, Jakob
A1  - Krane, Markus
A1  - Weiss, Günther
A1  - Tsimikas, Sotirios
A1  - Thanassoulis, George
A1  - Grimm, Michael
A1  - Rupp, Bernhard
A1  - Huber, Lukas A
A1  - Zhang, Shen-Ying
A1  - Casanova, Jean-Laurent
A1  - Tancevski, Ivan
A1  - Holfeld, Johannes
T1  - Toll-Like Receptor 3 Mediates Aortic Stenosis Through a Conserved Mechanism of Calcification
JF  - Circulation
KW  - Toll-like receptor 3
KW  - aortic valve disease
KW  - biglycan
KW  - extracellular matrix
KW  - osteogenesis
Y1  - 
U6  - http://dx.doi.org/10.1161/CIRCULATIONAHA.122.063481
VL  - 147
IS  - 20
SP  - 1518
EP  - 1533
ER  -