TY  - JOUR
A1  - Tallian, Claudia
A1  - Herrero-Rollett, Alexandra
A1  - Stadler, Karina
A1  - Vielnascher, Robert
A1  - Wieland, Karin
A1  - Weihs, Anna
A1  - Pellis, Alessandro
A1  - Teuschl, Andreas
A1  - Lendl, Bernhard
A1  - Amenitsch, Heinz
A1  - Guebitz, Georg M.
T1  - Structural insights into pH-responsive drug release of self-assembling human serum albumin-silk fibroin nanocapsules.
JF  - European Journal of Pharmaceutics and Biopharmaceutics
N2  - Inflammation processes are associated with significant decreases in tissue or lysosomal pH from 7.4 to 4, a fact that argues for the application of pH-responsive drug delivery systems. However, for their design and optimization a full understanding of the release mechanism is crucial. In this study we investigated the pH-depending drug release mechanism and the influence of silk fibroin (SF) concentration and SF degradation degree of human serum albumin (HSA)-SF nanocapsules. Sonochemically produced nanocapsules were investigated regarding particle size, colloidal stability, protein encapsulation, thermal stability and drug loading properties. Particles of the monodisperse phase showed average hydrodynamic radii between 438 and 888 nm as measured by DLS and AFM and a zeta potential of -11.12 ± 3.27 mV. Together with DSC results this indicated the successful production of stable nanocapsules. ATR-FTIR analysis demonstrated that SF had a positive effect on particle formation and stability due to induced beta-sheet formation and enhanced crosslinking. The pH-responsive release was found to depend on the SF concentration. In in-vitro release studies, HSA-SF nanocapsules composed of 50% SF showed an increased pH-responsive release for all tested model substances (Rhodamine B, Crystal Violet and Evans Blue) and methotrexate at the lowered pH of 4.5 to pH 5.4, while HSA capsules without SF did not show any pH-responsive drug release. Mechanistic studies using confocal laser scanning microscopy (CLSM) and small angle X-ray scattering (SAXS) analyses showed that increases in particle porosity and decreases in particle densities are directly linked to pH-responsive release properties. Therefore, the pH-responsive release mechanism was identified as diffusion controlled in a novel and unique approach by linking scattering results with in vitro studies. Finally, cytotoxicity studies using the human monocytic THP-1 cell line indicated non-toxic behavior of the drug loaded nanocapsules when applied in a concentration of 62.5 µg mL-1.
KW  - Biomaterial
KW  - Tissue Engineering
Y1  - 
ER  - 
TY  - GEN
A1  - Teuschl, Andreas
A1  - Schuh, Christina
A1  - Weihs, Anna
A1  - Guillaume, Olivier
A1  - Monforte Vila, Xavier
A1  - Redl, Heinz
A1  - Kaplan, David
A1  - Rünzler, Dominik
T1  - Tailoring bioactivity of silk-based biomaterials via delivering and functionalization strategies with fibrinogen/thrombin, plant lectins or laminin
KW  - Biomaterials
KW  - Tissue Engineering
KW  - Silk
Y1  - 
ER  - 
TY  - GEN
A1  - Salzer, Elias
A1  - Rieder, Bernhard
A1  - Monforte Vila, Xavier
A1  - Weihs, Anna
A1  - Rünzler, Dominik
A1  - Teuschl, Andreas
T1  - Evaluation of a novel hydrostatic pressure bioreactor on bovine cartilage chips
KW  - Bioreactor
KW  - Tissue Engineering
KW  - Cartilage
Y1  - 
ER  - 
TY  - GEN
A1  - Slezak, Paul
A1  - Rose, Roland
A1  - Hercher, David
A1  - Weihs, Anna
A1  - Fuchs, Christiane
A1  - Redl, Heinz
A1  - Mittermayr, Rainer
A1  - Slezak, Cyrill
T1  - Tracking therapeutic shockwaves and their impact on regeneration
KW  - Shockwave Therapy
KW  - Tissue Regeneration
Y1  - 
ER  - 
TY  - GEN
A1  - Hromada, Carina
A1  - Tomasch, Janine
A1  - Weihs, Anna
A1  - Rünzler, Dominik
A1  - Teuschl, Andreas
T1  - Engineering of 3D Tissue Constructs Using our Novel MagneTissue Bioreactor as Alternatives to Animal Models
KW  - Bioreactor
KW  - Biomaterials
Y1  - 
ER  - 
TY  - JOUR
A1  - Rieder, Bernhard
A1  - Weihs, Anna
A1  - Weidinger, Adelheid
A1  - Sczwarc, Dorota
A1  - Nürnberger, Sylvia
A1  - Redl, Heinz
A1  - Rünzler, Dominik
A1  - Huber-Gries, Carina
A1  - Teuschl, Andreas
T1  - Hydrostatic pressure-generated reactive oxygen species induce osteoarthritic conditions in cartilage pellet cultures
JF  - Scientific Reports
KW  - Bioreactor
KW  - Osteoarthritis
KW  - Cartilage
KW  - Reactive oxygen species
Y1  - 
ER  -