TY - JOUR A1 - Schuh, Christina A1 - Heher, Philipp A1 - Weihs, Anna A1 - Fuchs, Christiane A1 - Gabriel, Christian A1 - Wolbank, Susanne A1 - Mittermayr, Rainer A1 - Redl, Heinz A1 - Rünzler, Dominik A1 - Teuschl, Andreas T1 - In vitro extracorporeal shock wave treatment enhances stemness and preserves multipotency of rat and human adipose-derived stem cells JF - Journal of Cytotherapy KW - Shockwave Y1 - ER - TY - JOUR A1 - Simböck, Elisabeth A1 - Marksteiner, Jessica A1 - Machacek, Thomas A1 - Wiessner, Katharina A1 - Gepp, Barbara A1 - Jesenberger, Veronika A1 - Weihs, Anna A1 - Leitner, Rita T1 - The Power of Problem Based Learning beyond its Didactic Attributes JF - Journal of Problem Based Learning in Higher Education (JPBLHE) N2 - Hybrid courses with a focus on practice-orientated education and self-guided learning phases are on the rise on the higher education sector. Disciplines in Life Sciences implicate a high degree of practical laboratory expertise. The University of Applied Sciences (UAS) in Vienna, Austria, has thus been endeavoured offering students a high qualitative education integrating hybrid courses based on PBL principles, which consist of on-site (including the transmission of necessary background and practical laboratory training) and off-site (including self-study phases) sessions. As practical laboratory units are central in those courses, the restrictive measures, including the transition to a complete online teaching format due to the first Covid-19-pandemic lock-down, had severe effects on the implementation and the quality of the curriculum. According to surveys made specifically to address this problematic situation, it can be concluded that on-site practical units are fundamental for certain disciplines such as Life Sciences. KW - Problem-based Learning KW - Life Science didactics KW - Hybrid PBL-methods KW - COVID-19 KW - Life Science Education Y1 - VL - 9 IS - 1 SP - 109 EP - 130 ER - TY - JOUR A1 - Quartinello, Felice A1 - Tallian, Claudia A1 - Auer, Julia A1 - Schön, Herta A1 - Vielnascher, Robert A1 - Weinberger, Simone A1 - Wieland, Karin A1 - Weihs, Anna A1 - Rollett, Alexandra A1 - Lendl, Bernhard A1 - Teuschl, Andreas A1 - Pellis, Alessandro A1 - Gübitz, Georg T1 - Smart Textiles in Wound Care: Functionalization of Cotton/PET Blends with Antimicrobial Nanocapsules JF - Journal of Materials Chemistry B KW - Smart textiles KW - Tissue Engineering KW - Regenerative Medicine KW - Antimicrobial KW - Wound Y1 - 2019 ER - TY - JOUR A1 - Tallian, Claudia A1 - Herrero-Rollett, Alexandra A1 - Stadler, Karina A1 - Vielnascher, Robert A1 - Wieland, Karin A1 - Weihs, Anna A1 - Pellis, Alessandro A1 - Teuschl, Andreas A1 - Lendl, Bernhard A1 - Amenitsch, Heinz A1 - Guebitz, Georg M. T1 - Structural insights into pH-responsive drug release of self-assembling human serum albumin-silk fibroin nanocapsules. JF - European Journal of Pharmaceutics and Biopharmaceutics N2 - Inflammation processes are associated with significant decreases in tissue or lysosomal pH from 7.4 to 4, a fact that argues for the application of pH-responsive drug delivery systems. However, for their design and optimization a full understanding of the release mechanism is crucial. In this study we investigated the pH-depending drug release mechanism and the influence of silk fibroin (SF) concentration and SF degradation degree of human serum albumin (HSA)-SF nanocapsules. Sonochemically produced nanocapsules were investigated regarding particle size, colloidal stability, protein encapsulation, thermal stability and drug loading properties. Particles of the monodisperse phase showed average hydrodynamic radii between 438 and 888 nm as measured by DLS and AFM and a zeta potential of -11.12 ± 3.27 mV. Together with DSC results this indicated the successful production of stable nanocapsules. ATR-FTIR analysis demonstrated that SF had a positive effect on particle formation and stability due to induced beta-sheet formation and enhanced crosslinking. The pH-responsive release was found to depend on the SF concentration. In in-vitro release studies, HSA-SF nanocapsules composed of 50% SF showed an increased pH-responsive release for all tested model substances (Rhodamine B, Crystal Violet and Evans Blue) and methotrexate at the lowered pH of 4.5 to pH 5.4, while HSA capsules without SF did not show any pH-responsive drug release. Mechanistic studies using confocal laser scanning microscopy (CLSM) and small angle X-ray scattering (SAXS) analyses showed that increases in particle porosity and decreases in particle densities are directly linked to pH-responsive release properties. Therefore, the pH-responsive release mechanism was identified as diffusion controlled in a novel and unique approach by linking scattering results with in vitro studies. Finally, cytotoxicity studies using the human monocytic THP-1 cell line indicated non-toxic behavior of the drug loaded nanocapsules when applied in a concentration of 62.5 µg mL-1. KW - Biomaterial KW - Tissue Engineering Y1 - ER - TY - JOUR A1 - Weihs, Anna A1 - Fuchs, Christiane A1 - Teuschl, Andreas A1 - Hartinger, Joachim A1 - Slezak, Paul A1 - Mittermayr, Rainer A1 - Redl, Heinz A1 - Junger, Wolfgang A1 - Sitte, Harald A1 - Rünzler, Dominik T1 - Shock Wave Treatment Enhances Cell Proliferation and Improves Wound Healing by ATP Release-coupled Extracellular Signal-regulated Kinase (ERK) Activation JF - The Journal of biological chemistry KW - Shockwave Y1 - ER - TY - JOUR A1 - Schuh, Christina A1 - Heher, Philipp A1 - Weihs, Anna A1 - Asmita, Banerjee A1 - Wolbank, Susanne A1 - Mittermayr, Rainer A1 - Redl, Heinz A1 - Rünzler, Dominik A1 - Teuschl, Andreas T1 - Adipose derived stem cells respond to in vitro extracorporeal shockwave treatment with increased stemness and multipotency JF - New Biotechnology KW - Shockwave Y1 - ER - TY - JOUR A1 - Rieder, Bernhard A1 - Weihs, Anna A1 - Teuschl, Andreas A1 - Knebl, Gerald A1 - Kollmitzer, Josef A1 - Redl, Heinz A1 - Rünzler, Dominik T1 - Evaluation of cell response on permanent and pulsed atmospheric pressure stressed cells JF - Journal of Tissue Engineering and Regenerative Medicine KW - Cells KW - Atmospheric Pressure Y1 - 2018 VL - 1 IS - 6 SP - 240 EP - 240 ER - TY - JOUR A1 - Weihs, Anna A1 - Junger, Wolfgang A1 - Schaden, Wolfgang A1 - Sitte, Harald A1 - Rünzler, Dominik T1 - Extracorporeal shockwave treatment induced extracellular ATP release - a potential mechanism to activate wound healing JF - Journal of Tissue Engineering and Regenerative Medicine KW - Shockwave treatment KW - Healing Processes Y1 - 2018 VL - 1 IS - 6 SP - 381 EP - 381 ER - TY - JOUR A1 - Rünzler, Dominik A1 - Weihs, Anna A1 - Junger, Wolfgang A1 - Sitte, Harald A1 - Schaden, Wolfgang T1 - Extracorporeal shock waves - a novel method of mechanostimulation in tissue regeneration JF - Journal of Tissue Engineering and Regenerative Medicine KW - Shockwave treatment KW - Mechanostimulation Y1 - 2018 VL - 1 IS - 6 SP - 380 EP - 380 ER - TY - JOUR A1 - Rieder, Bernhard A1 - Weihs, Anna A1 - Weidinger, Adelheid A1 - Sczwarc, Dorota A1 - Nürnberger, Sylvia A1 - Redl, Heinz A1 - Rünzler, Dominik A1 - Huber-Gries, Carina A1 - Teuschl, Andreas T1 - Hydrostatic pressure-generated reactive oxygen species induce osteoarthritic conditions in cartilage pellet cultures JF - Scientific Reports KW - Bioreactor KW - Osteoarthritis KW - Cartilage KW - Reactive oxygen species Y1 - ER -