TY  - GEN
A1  - Teuschl, Andreas
A1  - Schuh, Christina
A1  - Weihs, Anna
A1  - Guillaume, Olivier
A1  - Monforte Vila, Xavier
A1  - Redl, Heinz
A1  - Kaplan, David
A1  - Rünzler, Dominik
T1  - Tailoring bioactivity of silk-based biomaterials via delivering and functionalization strategies with fibrinogen/thrombin, plant lectins or laminin
KW  - Biomaterials
KW  - Tissue Engineering
KW  - Silk
Y1  - 
ER  - 
TY  - GEN
A1  - Teuschl, Andreas
A1  - Weihs, Anna
A1  - Fuchs, Christiane
A1  - Monforte Vila, Xavier
T1  - Silk as a versatile biomaterial for musculoskeletal tissue engineering
KW  - Silk
KW  - Biomaterials
Y1  - 2018
ER  - 
TY  - GEN
A1  - Hromada, Carina
A1  - Tomasch, Janine
A1  - Weihs, Anna
A1  - Rünzler, Dominik
A1  - Teuschl, Andreas
T1  - Engineering of 3D Tissue Constructs Using our Novel MagneTissue Bioreactor as Alternatives to Animal Models
KW  - Bioreactor
KW  - Biomaterials
Y1  - 
ER  - 
TY  - JOUR
A1  - Johannes, Hackethal
A1  - Weihs, Anna
A1  - Karner, Lisa
A1  - Metzger, Magdalena
A1  - Dungel, Peter
A1  - Hennerbichler, Simone
A1  - Redl, Heinz
A1  - Teuschl-Woller, Andreas Herbert
T1  - Novel Human Placenta-Based Extract for Vascularization Strategies in Tissue Engineering
JF  - Tissue Eng Part C Methods
N2  - There is critical unmet need for new vascularized tissues to support or replace injured tissues and organs. Various synthetic and natural materials were already established for use of two-dimensional (2D) and three-dimensional (3D) in vitro neovascularization assays, however, they still cannot mimic the complex functions of the sum of the extracellular matrix (ECM) in native intact tissue. Currently, this issue is only addressed by artificial products such as Matrigel™, which comprises a complex mixture of ECM proteins, extracted from animal tumor tissue. Despite its outstanding bioactivity, the isolation from tumor tissue hinders its translation into clinical applications. Since nonhuman ECM proteins may cause immune reactions, as are frequently observed in clinical trials, human ECM proteins represent the best option when aiming for clinical applications. Here, we describe an effective method of isolating a human placenta substrate (hpS) that induces the spontaneous formation of an interconnected network of green fluorescence-labeled human umbilical vein endothelial cells (gfpHUVECs) in vitro. The substrate was biochemically characterized by using a combination of bicinchoninic acid (BCA) assay, DNA, and glycosaminoglycan (GAG) content assays, sodium dodecyl sulfate/polyacrylamide gel electrophoresis (SDS-PAGE) analysis and Western blot, angiogenesis arrays, chromatographic thrombin detection, high performance liquid chromatography (HPLC)-based amino acid quantification analysis, and assessment of antimicrobial properties. 2D in vitro cell culture experiments have been performed to determine the vasculogenic potential of hpS, which demonstrated that cell networks developed on hpS show a significantly higher degree of complexity (number of tubules/junctions; total/mean tube length) when compared with Matrigel. As 3D cell culture techniques represent a more accurate representation of the in vivo condition, the substrate was 3D solidified using various natural polymers. 3D in vitro vasculogenesis assays have been performed by seeding gfpHUVECs in an hpS-fibrinogen clot. In conclusion, hpS provides a potent human/material-based alternative to xenogenic-material-based biomaterials for vascularization strategies in tissue engineering.
KW  - Tissue Engineering
KW  - Biomaterials
KW  - HUVEC
KW  - Acellular biological matrices
KW  - Angiogenesis and vasculogenesis
Y1  - 
VL  - 27
IS  - 11
SP  - 616
EP  - 632
ER  -