TY  - JOUR
A1  - Berkovitch, Yulia
A1  - Cohen, Talia
A1  - Peled, Eli
A1  - Schmidhammer, Robert
A1  - Hildner, Florian
A1  - Teuschl, Andreas
A1  - Wolbank, Susanne
A1  - Yelin, Dvir
A1  - Redl, Heinz
A1  - Seliktar, Dror
T1  - Hydrogel composition and laser micropatterning to regulate sciatic nerve regeneration.
JF  - Journal of Tissue Engineering and Regenerative Medicine
N2  - Treatment of peripheral nerve injuries has evolved over the past several decades to include the use of sophisticated new materials endowed with trophic and topographical cues that are essential for in vivo nerve fibre regeneration. In this research, we explored the use of an advanced design strategy for peripheral nerve repair, using biological and semi-synthetic hydrogels that enable controlled environmental stimuli to regenerate neurons and glial cells in a rat sciatic nerve resection model. The provisional nerve growth conduits were composed of either natural fibrin or adducts of synthetic polyethylene glycol and fibrinogen or gelatin. A photo-patterning technique was further applied to these 3D hydrogel biomaterials, in the form of laser-ablated microchannels, to provide contact guidance for unidirectional growth following sciatic nerve injury. We tested the regeneration capacity of subcritical nerve gap injuries in rats treated with photo-patterned materials and compared these with injuries treated with unpatterned hydrogels, either stiff or compliant. Among the factors tested were shear modulus, biological composition, and micropatterning of the materials. The microchannel guidance patterns, combined with appropriately matched degradation and stiffness properties of the material, proved most essential for the uniform tissue propagation during the nerve regeneration process.
KW  - Tissue Engineering
KW  - Biomaterials
KW  - Nerve Regeneration
Y1  - 
SP  - 1049
EP  - 1061
ER  - 
TY  - JOUR
A1  - Nürnberger, Sylvia
A1  - Schneider, Cornelia
A1  - van Osch, Gerjo
A1  - Keibl, Claudia
A1  - Rieder, Bernhard
A1  - Monforte, Xavier
A1  - Teuschl, Andreas
A1  - Mühleder, Severin
A1  - Holnthoner, Wolfgang
A1  - Schädl, Barbara
A1  - Gahleitner, Christoph
A1  - Redl, Heinz
A1  - Wolbank, Susanne
T1  - Repopulation of an auricular cartilage scaffold, AuriScaff, perforated with an enzyme combination.
JF  - Acta Biomaterialia
KW  - Tissue Engineering
KW  - Decellularization
KW  - Cartilage
Y1  - 
ER  - 
TY  - JOUR
A1  - Strohmeier, Karin
A1  - Hofmann, Martina
A1  - Jacak, Jaroslaw
A1  - Narzt, Marie-Sophie
A1  - Wahlmueller, Marlene
A1  - Mairhofer, Mario
A1  - Schädl, Barbara
A1  - Holnthoner, Wolfgang
A1  - Barsch, Martin
A1  - Sandhofer, Matthias
A1  - Wolbank, Susanne
A1  - Priglinger, Eleni
T1  - Multi-Level Analysis of Adipose Tissue Reveals the Relevance of Perivascular Subpopulations and an Increased Endothelial Permeability in Early-Stage Lipedema
JF  - Biomedicines
N2  - Lipedema is a chronic, progressive disease of adipose tissue with unknown etiology. Based on the relevance of the stromal vascular fraction (SVF) cell population in lipedema, we performed a thorough characterization of subcutaneous adipose tissue, SVF isolated thereof and the sorted populations of endothelial cells (EC), pericytes and cultured adipose-derived stromal/stem cells (ASC) of early-stage lipedema patients. We employed histological and gene expression analysis and investigated the endothelial barrier by immunofluorescence and analysis of endothelial permeability in vitro. Although there were no significant differences in histological stainings, we found altered gene expression of factors relevant for local estrogen metabolism (aromatase), preadipocyte commitment (ZNF423) and immune cell infiltration (CD11c) in lipedema on the tissue level, as well as in distinct cellular subpopulations. Machine learning analysis of immunofluorescence images of CD31 and ZO-1 revealed a morphological difference in the cellular junctions of EC cultures derived from healthy and lipedema individuals. Furthermore, the secretome of lipedema-derived SVF cells was sufficient to significantly increase leakiness of healthy human primary EC, which was also reflected by decreased mRNA expression of VE-cadherin. Here, we showed for the first time that the secretome of SVF cells creates an environment that triggers endothelial barrier dysfunction in early-stage lipedema. Moreover, since alterations in gene expression were detected on the cellular and/or tissue level, the choice of sample material is of high importance in elucidating this complex disease.
KW  - Tissue Engineering
KW  - Adipose Tissue
KW  - Lipedema
KW  - Endothelial Cells
Y1  - 
VL  - 2022
IS  - 10(5)
SP  - 1163
ER  -