TY - CHAP A1 - Salzbrunn, Benedikt A1 - Busch, Marc A1 - della Schiava, Marco A1 - Kipke, Christine T1 - rapid UX-score: Modulare und Adaptive Messung von User Experience T2 - Mensch und Computer 2020 - Workshopband KW - UX Messung KW - Modulare UX Messung KW - Adaptive UX Messung KW - Rasch-Modell Y1 - ER - TY - CHAP A1 - Mally, Franziska A1 - Hofstätter, Otto A1 - Eckelt, Markus T1 - Influence of Running Shoes and Running Velocity on “Ride” during Running. T2 - ISEA 2020 Online - Proceedings 2020 KW - Sports Equipment Technologies KW - Running Shoes KW - Footwear Y1 - 2020 ER - TY - JOUR A1 - Purtscher, Michaela A1 - Rothbauer, Mario A1 - Kratz, Sebastian Rudi Adam A1 - Bailey, Andrew A1 - Lieberzeit, Peter A1 - Ertl, Peter T1 - A microfluidic impedance-based extended infectivity assay: combining retroviral amplification and cytopathic effect monitoring on a single lab-on-a-chip platform JF - Lab on a Chip N2 - Detection, quantification and monitoring of virus – host cell interactions are of great importance when evaluating the safety of pharmaceutical products. With the wide usage of viral based vector systems in combination with mammalian cell lines for the production of biopharmaceuticals, the presence of replication competent viral particles needs to be avoided and potential hazards carefully assessed. Consequently, regulatory agencies recommend viral clearance studies using plaque assays or TCID50 assays to evaluate the efficiency of the production process in removing viruses. While plaque assays provide reliable information on the presence of viral contaminations, they are still tedious to perform and can take up to two weeks to finish. To overcome some of these limitations, we have automated, miniaturized and integrated the dual cell culture bioassay into a common lab-on-a-chip platform containing embedded electrical sensor arrays to enrich and detect infectious viruses. Results of our microfluidic single step assay show that a significant reduction in assay time down to 3 to 4 days can be achieved using simultaneous cell-based viral amplification, release and detection of cytopathic effects in a target cell line. We further demonstrate the enhancing effect of continuous fluid flow on infection of PG-4 reporter cells by newly formed and highly active virions by M. dunni cells, thus pointing to the importance of physical relevant viral–cell interactions. KW - Tissue Engineering KW - Microfluidics KW - Lab on Chip Y1 - 2021 VL - 2021 IS - Issue 7 SP - 1364 EP - 1372 ER - TY - JOUR A1 - Deininger, Christian A1 - Wagner, Andrea A1 - Heimel, Patrick A1 - Salzer, Elias A1 - Monforte Vila, Xavier A1 - Weißenbacher, Nadja A1 - Grillari, Johannes A1 - Redl, Heinz A1 - Wichlas, Florian A1 - Freude, Thomas A1 - Tempfer, Herbert A1 - Teuschl-Woller, Andreas A1 - Traweger, Andreas T1 - Enhanced BMP-2-Mediated Bone Repair Using an Anisotropic Silk Fibroin Scaffold Coated with Bone-like Apatite JF - Int. J. Mol. Sci. N2 - The repair of large bone defects remains challenging and often requires graft material due to limited availability of autologous bone. In clinical settings, collagen sponges loaded with excessive amounts of bone morphogenetic protein 2 (rhBMP-2) are occasionally used for the treatment of bone non-unions, increasing the risk of adverse events. Therefore, strategies to reduce rhBMP-2 dosage are desirable. Silk scaffolds show great promise due to their favorable biocompatibility and their utility for various biofabrication methods. For this study, we generated silk scaffolds with axially aligned pores, which were subsequently treated with 10× simulated body fluid (SBF) to generate an apatitic calcium phosphate coating. Using a rat femoral critical sized defect model (CSD) we evaluated if the resulting scaffold allows the reduction of BMP-2 dosage to promote efficient bone repair by providing appropriate guidance cues. Highly porous, anisotropic silk scaffolds were produced, demonstrating good cytocompatibility in vitro and treatment with 10× SBF resulted in efficient surface coating. In vivo, the coated silk scaffolds loaded with a low dose of rhBMP-2 demonstrated significantly improved bone regeneration when compared to the unmineralized scaffold. Overall, our findings show that this simple and cost-efficient technique yields scaffolds that enhance rhBMP-2 mediated bone healing. KW - Tissue Engineering KW - Biomaterials KW - silk scaffold KW - bone regeneration KW - pseudoarthrosis Y1 - VL - 23 IS - 1 / 283 ER - TY - JOUR A1 - Hackethal, Johannes A1 - Dungel, Peter A1 - Teuschl, Andreas Herbert T1 - Frequently Used Strategies to Isolate Extracellular Matrix Proteins from Human Placenta and Adipose Tissue JF - Tissue Engineering Part C: Methods N2 - The natural extracellular matrix (ECM) provides the optimal environment for cells. Many enzymatic or non-enzymatic based strategies to extract ECM proteins from tissues were published over the past years. However, every single isolation strategy reported so far is associated with specific bottlenecks. In this study, frequently used strategies to isolate ECM from human placenta or adipose tissue using Tris-, serum-, or pepsin-based buffers were compared. The resulting ECM proteins were biochemically characterized by analysis of cellular remnants using Hoechst DNA staining, glycosaminoglycan (GAG) content by dimethylmethylene blue, visualization of protein bands using sodium dodecyl sulfate-polyacrylamide gel electrophoresis analysis combined with amino acid quantification, and assessment of the proangiogenic profile using an angiogenesis array. Tris-NaCl-extracted ECM proteins showed a high heterogenic degree of extracted proteins, bioactive growth factors, and GAGs, but no collagen-I. Active serum-extracted ECM showed significant lower DNA remnants when compared with the Tris-NaCl isolation strategy. Pepsin-extracted ECM was rich in collagen-I and low amounts of remaining bioactive growth factors. This strategy was most effective to reduce DNA amounts when compared with the other isolation strategies. Pepsin-extracted ECM from both tissues easily gelled at 37°C, whereas the other extracted ECM strategies did not gel at 37°C (Tris-NaCl: liquid; serum: sponge). All relevant characteristics (DNA residues, ECM diversity and bioactivity, shape) of the extracted ECM proteins highly depend on its isolation strategy and could still be optimized. Impact statement The natural human extracellular matrix (ECM) is the ideal cell niche. Various strategies were reported to isolate human ECM components from various sources. In this article, we compared frequently used methods and compared their characteristics (DNA remnants, glycosaminoglycan content, sodium dodecyl sulfate-polyacrylamide gel electrophoresis analysis, amino acid quantification, angiogenesis array, and gel formation). We conclude that more research is still necessary to optimize current isolation approaches for in vitro or in vivo applications of human ECM. KW - Tissue Engineering KW - Biomaterials KW - Adipose Tissue KW - extracellular matrix KW - human placenta Y1 - VL - 27 IS - 12 SP - 649 EP - 660 ER - TY - JOUR A1 - Khimich, Margarita A. A1 - Prosolov, Konstantin A. A1 - Mishurova, Tatiana A1 - Evsevleev, Sergej A1 - Monforte, Xavier A1 - Teuschl, Andreas H. A1 - Slezak, Paul A1 - Ibragimov, Egor A. A1 - Saprykin, Alexander A. A1 - Kovalevskaya, Zhanna G. A1 - Dmitriev, Andrey I. A1 - Bruno, Giovanni A1 - Sharkeev, Yurii P. T1 - Advances in Laser Additive Manufacturing of Ti-Nb Alloys: From Nanostructured Powders to Bulk Objects JF - Nanomaterials (Basel) N2 - The additive manufacturing of low elastic modulus alloys that have a certain level of porosity for biomedical needs is a growing area of research. Here, we show the results of manufacturing of porous and dense samples by a laser powder bed fusion (LPBF) of Ti-Nb alloy, using two distinctive fusion strategies. The nanostructured Ti-Nb alloy powders were produced by mechanical alloying and have a nanostructured state with nanosized grains up to 90 nm. The manufactured porous samples have pronounced open porosity and advanced roughness, contrary to dense samples with a relatively smooth surface profile. The structure of both types of samples after LPBF is formed by uniaxial grains having micro- and nanosized features. The inner structure of the porous samples is comprised of an open interconnected system of pores. The volume fraction of isolated porosity is 2 vol. % and the total porosity is 20 vol. %. Cell viability was assessed in vitro for 3 and 7 days using the MG63 cell line. With longer culture periods, cells showed an increased cell density over the entire surface of a porous Ti-Nb sample. Both types of samples are not cytotoxic and could be used for further in vivo studies. KW - Tissue Engineering KW - Biomaterials KW - Laser Additive Manufacturing KW - Bulk Objects Y1 - VL - 11 IS - 5 / 1159 ER - TY - JOUR A1 - Farokhi, Maryam A1 - Aleemardani, Mina A1 - Solouk, Atefeh A1 - Mirzadeh, Hamid A1 - Teuschl, Andreas Herbert A1 - Redl, Heinz T1 - Crosslinking strategies for silk fibroin hydrogels: promising biomedical materials JF - Biomedical Materials N2 - Due to their strong biomimetic potential, silk fibroin (SF) hydrogels are impressive candidates for tissue engineering, due to their tunable mechanical properties, biocompatibility, low immunotoxicity, controllable biodegradability, and a remarkable capacity for biomaterial modification and the realization of a specific molecular structure. The fundamental chemical and physical structure of SF allows its structure to be altered using various crosslinking strategies. The established crosslinking methods enable the formation of three-dimensional (3D) networks under physiological conditions. There are different chemical and physical crosslinking mechanisms available for the generation of SF hydrogels (SFHs). These methods, either chemical or physical, change the structure of SF and improve its mechanical stability, although each method has its advantages and disadvantages. While chemical crosslinking agents guarantee the mechanical strength of SFH through the generation of covalent bonds, they could cause some toxicity, and their usage is not compatible with a cell-friendly technology. On the other hand, physical crosslinking approaches have been implemented in the absence of chemical solvents by the induction of β-sheet conformation in the SF structure. Unfortunately, it is not easy to control the shape and properties of SFHs when using this method. The current review discusses the different crosslinking mechanisms of SFH in detail, in order to support the development of engineered SFHs for biomedical applications. KW - Tissue Engineering KW - hydrogels KW - Biomaterials KW - silk fibroin Y1 - VL - 16 IS - 2 SP - 022004 ER - TY - CHAP A1 - Mally, Franziska T1 - In-Shoe Plantar Pressure Measurement—Influence of Insole Placement on Selected Parameters during Running. T2 - ISEA 2020 Online - Proceedings 2020, 49(1) KW - Sports Equipment Technologies KW - Running Shoes KW - Motion Analysis Y1 - 2020 SP - 50 EP - 50 ER - TY - JOUR A1 - Ziadlou, Reihane A1 - Rotman, Stijn A1 - Teuschl, Andreas A1 - Salzer, Elias A1 - Barbero, Andrea A1 - Martin, Ivan A1 - Alini, Mauro A1 - Eglin, David A1 - Grad, Sibylle T1 - Optimization of hyaluronic acid-tyramine/silk-fibroin composite hydrogels for cartilage tissue engineering and delivery of anti-inflammatory and anabolic drugs JF - Materials Science and Engineering: C N2 - Injury of articular cartilage leads to an imbalance in tissue homeostasis, and due to the poor self-healing capacity of cartilage the affected tissue often exhibits osteoarthritic changes. In recent years, injectable and highly tunable composite hydrogels for cartilage tissue engineering and drug delivery have been introduced as a desirable alternative to invasive treatments. In this study, we aimed to formulate injectable hydrogels for drug delivery and cartilage tissue engineering by combining different concentrations of hyaluronic acid-tyramine (HA-Tyr) with regenerated silk-fibroin (SF) solutions. Upon enzymatic crosslinking, the gelation and mechanical properties were characterized over time. To evaluate the effect of the hydrogel compositions and properties on extracellular matrix (ECM) deposition, bovine chondrocytes were embedded in enzymatically crosslinked HA-Tyr/SF composites (in further work abbreviated as HA/SF) or HA-Tyr hydrogels. We demonstrated that all hydrogel formulations were cytocompatible and could promote the expression of cartilage matrix proteins allowing chondrocytes to produce ECM, while the most prominent chondrogenic effects were observed in hydrogels with HA20/SF80 polymeric ratios. Unconfined mechanical testing showed that the compressive modulus for HA20/SF80 chondrocyte-laden constructs was increased almost 10-fold over 28 days of culture in chondrogenic medium which confirmed the superior production of ECM in this hydrogel compared to other hydrogels in this study. Furthermore, in hydrogels loaded with anabolic and anti-inflammatory drugs, HA20/SF80 hydrogel showed the longest and the most sustained release profile over time which is desirable for the long treatment duration typically necessary for osteoarthritic joints. In conclusion, HA20/SF80 hydrogel was successfully established as a suitable injectable biomaterial for cartilage tissue engineering and drug delivery applications. KW - Tissue Engineering KW - Cartilage KW - Mechanical Testing KW - Biomaterials KW - Chondrocytes Y1 - VL - 120 IS - 111701 ER - TY - JOUR A1 - Frohner, Matthias A1 - Mense, Alexander A1 - Krondraf, Nikolaus A1 - Sabutsch, Stefan T1 - Implementierungsleitfaden HL7 Implementation Guide for CDA® R2: Implementierungsleitfaden Meldung an das Epidemiologische Meldesystem (EMS) – Labor-und Arztmeldung, ELGA GmbH JF - ELGA CDA Implementierungsleitfäden HL7 KW - eHealth KW - ELGA Y1 - VL - 2020 ER - TY - JOUR A1 - Frohner, Matthias A1 - Sabutsch, Stefan A1 - Krondraf, Nikolaus T1 - ELGA CDA Implementierungsleitfäden HL7 Implementation Guide for CDA® R2: Ambulanzbefund, ELGA GmbH JF - ELGA CDA Implementierungsleitfäden HL7 KW - eHealth KW - ELGA Y1 - VL - 2020 ER - TY - CHAP A1 - Eckelt, Markus A1 - Mally, Franziska T1 - Running Shoes—Possible Correlations of Biomechanical and Material Tests. T2 - ISEA 2020 Online - Proceedings 2020, 49(1) KW - Sports Equipment Technologies KW - Running Shoes Y1 - 2020 SP - 25 EP - 25 ER - TY - CHAP A1 - Eckelt, Markus A1 - Mally, Franziska T1 - Use of Acceleration Sensors in Archery T2 - ISEA 2020 Online - Proceedings 2020, 49(1) KW - Sports Equipment Technologies KW - Acceleration Sensors KW - Archery Y1 - 2020 SP - 98 EP - 98 ER - TY - JOUR A1 - Jesenberger, Veronika A1 - Zmajkovicova, Katarina A1 - Catalanotti, Federica A1 - Baumgartner, Christian A1 - Reyes, Gloria Ximena A1 - Baccarini, Manuela T1 - MEK1 is required for PTEN membrane recruitment, AKT regulation, and the maintenance of peripheral tolerance JF - Mol. Cell N2 - The Raf/MEK/ERK and PI3K/Akt pathways are prominent effectors of oncogenic Ras. These pathways negatively regulate each other, but the mechanism involved is incompletely understood. We now identify MEK1 as an essential regulator of lipid/protein phosphatase PTEN, through which it controls phosphatidylinositol-3-phosphate accumulation and AKT signaling. MEK1 ablation stabilizes AKT activation and, in vivo, causes a lupus-like autoimmune disease and myeloproliferation. Mechanistically, MEK1 is necessary for PTEN membrane recruitment as part of a ternary complex containing the multidomain adaptor MAGI1. Complex formation is independent of MEK1 kinase activity but requires phosphorylation of T292 on MEK1 by activated ERK. Thus, inhibiting the ERK pathway reduces PTEN membrane recruitment, increasing phosphatidylinositol-3-phosphate accumulation and AKT activation. Our data offer a conceptual framework for the observation that activation of the PI3K pathway frequently mediate resistance to MEK inhibitors and for the promising results obtained by combined MEK/PI3K inhibition in preclinical cancer models. KW - MEK1 pathway KW - membrane recruitment KW - preclinical cancer models Y1 - 2020 VL - 2013 IS - 50 SP - 43 EP - 55 ER - TY - JOUR A1 - Catalanotti, Federica A1 - Reyes, Gloria Ximena A1 - Jesenberger, Veronika A1 - Galabova-Kovacs, Gergana A1 - de Matos Simoes, Ricardo A1 - Carugo, Oliviero A1 - Baccarini, Manuela T1 - A Mek1-Mek2 heterodimer determines the strength and duration of the Erk signal JF - Nat Struct Mol Biol. N2 - Mek1 and Mek2 (also known as Map2k1 and Map2k2, respectively) are evolutionarily conserved, dual-specificity kinases that mediate Erk1 and Erk2 activation during adhesion and growth factor signaling. Here we describe a previously uncharacterized, unexpected role of Mek1 in downregulating Mek2-dependent Erk signaling. Mek1 mediates the regulation of Mek2 in the context of a previously undiscovered Mek1-Mek2 complex. The Mek heterodimer is negatively regulated by Erk-mediated phosphorylation of Mek1 on Thr292, a residue missing in Mek2. Disabling this Erk-proximal negative-feedback step stabilizes the phosphorylation of both Mek2 and Erk in cultured cells and in vivo in Mek1 knockout embryos and mice. Thus, in disagreement with the current perception of the pathway, the role of Mek1 and Mek2 in growth factor-induced Erk phosphorylation is not interchangeable. Our data establish Mek1 as the crucial modulator of Mek and Erk signaling and have potential implications for the role of Mek1 and Mek2 in tumorigenesis. KW - Mek1-Mek2 pathway KW - tumorigenesis KW - ERK activation KW - cancer biology Y1 - 2020 VL - 2009 IS - 16(3) SP - 294 EP - 303 ER - TY - CHAP A1 - Pescha, David A1 - Horauer, Martin T1 - Eventroms in IEC 61499 Applications T2 - Proceedings of XXXIII Conference on Design of Circuits and Integrated Systems (DCIS ’2018), November 14-16, 2018, Lyon, France KW - IEC Standards KW - Storms KW - Sensors KW - Monitoring KW - Hardware Y1 - 2019 SN - 978-1-7281-0171-2 ER - TY - CHAP A1 - Dolezal, Dominik T1 - Educational Design Patterns for Student-Centered 21st Century Learning in Technology-Enhanced Learning Environments T2 - Educating Engineers for Future Industrial Revolutions: Proceedings of the 23rd International Conference on Interactive Collaborative Learning (ICL2020) KW - 21st century skills KW - student-centered learning KW - educational design patterns Y1 - ER - TY - CHAP A1 - Dolezal, Dominik T1 - The Learning Office Approach to Modern 21st Century Education: Third Evaluation of a Self-Driven Concept for Student-Centered Engineering Education T2 - IEEE 50th Annual Frontiers in Education Conference KW - learning office KW - 21st century skills KW - student-centered learning KW - computer science education KW - person-centered approach Y1 - ER - TY - JOUR A1 - Krautwald, Stefan A1 - Büscher, Dirk A1 - Jesenberger, Veronika A1 - Buder, Sylke A1 - Baccarini, Manuela T1 - Involvement of the protein tyrosine phosphatase SHP-1 in Ras-mediated activation of the mitogen-activated protein kinase pathway. JF - Mol Cell Biol. N2 - Ubiquitously expressed SH2-containing tyrosine phosphatases interact physically with tyrosine kinase receptors or their substrates and relay positive mitogenic signals via the activation of the Ras-mitogen-activated protein kinase (MAPK) pathway. Conversely, the structurally related phosphatase SHP-1 is predominantly expressed in hemopoietic cells and becomes tyrosine phosphorylated upon colony-stimulating factor 1 treatment of macrophages without associating with the colony-stimulating factor 1 receptor tyrosine kinase. Mice lacking functional SHP-1 (me/me and me(v)/me(v)) develop systemic autoimmune disease with accumulation of macrophages, suggesting that SHP-1 may be a negative regulator of hemopoietic cell growth. By using macrophages expressing dominant negative Ras and the me(v)/me(v) mouse mutant, we show that SHP-1 is activated in the course of mitogenic signal transduction in a Ras-dependent manner and that its activity is necessary for the Ras-dependent activation of the MAPK pathway but not of the Raf-1 kinase. Consistent with a role for SHP-1 as an intermediate between Ras and the MEK-MAPK pathway, Ras-independent activation of the latter kinases by bacterial lipopolysaccharide occurred normally in me(v)/me(v) cells. Our results sharply accentuate the diversity of signal transduction in mammalian cells, in which the same signaling intermediates can be rearranged to form different pathways. KW - Molecular Cell Biology KW - protein kinase pathway KW - Ras Y1 - 2020 U6 - http://dx.doi.org/doi: 10.1128/mcb.16.11.5955 VL - 1996 IS - 16(11) SP - 5955 EP - 5963 ER - TY - JOUR A1 - Jesenberger, Veronika A1 - Procyk, Katarzyna A1 - Yuan, Junying A1 - Reipert, Siegfried A1 - Baccarini, Manuela T1 - Salmonella-induced caspase-2 activation in macrophages: a novel mechanism in pathogen-mediated apoptosis JF - J Exp Med. N2 - The enterobacterial pathogen Salmonella induces phagocyte apoptosis in vitro and in vivo. These bacteria use a specialized type III secretion system to export a virulence factor, SipB, which directly activates the host's apoptotic machinery by targeting caspase-1. Caspase-1 is not involved in most apoptotic processes but plays a major role in cytokine maturation. We show that caspase-1-deficient macrophages undergo apoptosis within 4-6 h of infection with invasive bacteria. This process requires SipB, implying that this protein can initiate the apoptotic machinery by regulating components distinct from caspase-1. Invasive Salmonella typhimurium targets caspase-2 simultaneously with, but independently of, caspase-1. Besides caspase-2, the caspase-1-independent pathway involves the activation of caspase-3, -6, and -8 and the release of cytochrome c from mitochondria, none of which occurs during caspase-1-dependent apoptosis. By using caspase-2 knockout macrophages and chemical inhibition, we establish a role for caspase-2 in both caspase-1-dependent and -independent apoptosis. Particularly, activation of caspase-1 during fast Salmonella-induced apoptosis partially relies on caspase-2. The ability of Salmonella to induce caspase-1-independent macrophage apoptosis may play a role in situations in which activation of this protease is either prevented or uncoupled from the induction of apoptosis. KW - caspase-2 activation in macrophages KW - pathogen-activated apoptosis KW - Molecular Cell Biology Y1 - 2020 U6 - http://dx.doi.org/10.1084/jem.192.7.1035 VL - 2000 IS - 192(7) SP - 1035 EP - 1046 ER - TY - JOUR A1 - Jesenberger, Veronika A1 - Procyk, Katarzyna A1 - Rüth, Jochen A1 - Schreiber, Martin A1 - Theussl, Hans Christian A1 - Wagner, Erwin A1 - Baccarini, Manuela T1 - Protective Role of Raf-1 in Salmonella-Induced Macrophage Apoptosis JF - J Exp Med. N2 - Invasive Salmonella induces macrophage apoptosis via the activation of caspase-1 by the bacterial protein SipB. Here we show that infection of macrophages with Salmonella causes the activation and degradation of Raf-1, an important intermediate in macrophage proliferation and activation. Raf-1 degradation is SipB- and caspase-1–dependent, and is prevented by proteasome inhibitors. To study the functional significance of Raf-1 in this process, the c-raf-1 gene was inactivated by Cre-loxP–mediated recombination in vivo. Macrophages lacking c-raf-1 are hypersensitive towards pathogen-induced apoptosis. Surprisingly, activation of the antiapoptotic mitogen-activated protein kinase kinase (MEK)/extracellular signal–regulated kinase (ERK) and nuclear factor κB pathways is normal in Raf-1–deficient macrophages, and mitochondrial fragility is not increased. Instead, pathogen-mediated activation of caspase-1 is enhanced selectively, implying that Raf-1 antagonizes stimulus-induced caspase-1 activation and apoptosis. KW - Salmonella-Induced Macrophage Apoptosis KW - Raf-1 pathway KW - Molecular Cell Biology Y1 - 2020 U6 - http://dx.doi.org/10.1084/jem.193.3.353 VL - 2001 IS - 193(3) SP - 353 EP - 364 ER - TY - JOUR A1 - Jesenberger, Veronika A1 - Jentsch, Stefan T1 - Deadly encounter: ubiquitin meets apoptosis JF - Nat Rev Mol Cell Biol. N2 - The ubiquitin/proteasome pathway is the main non-lysosomal route for intracellular protein degradation in eukaryotes. It is instrumental to various cellular processes, such as cell-cycle progression, transcription and antigen processing. Recent findings also substantiate a pivotal role of the ubiquitin/proteasome pathway in the regulation of apoptosis. Regulatory molecules that are involved in programmed cell death have been identified as substrates of the proteasome. Moreover, key regulators of apoptosis themselves seem to have an active part in the proteolytic inactivation of death executors. KW - programmed cell death KW - Molecular Cell Biology Y1 - 2020 U6 - http://dx.doi.org/https://doi.org/10.1038/nrm731 VL - 2002 IS - 3(2) SP - 112 EP - 121 ER - TY - CHAP A1 - Litzenberger, Stefan T1 - A Study of Skin-Close Heat and Moisture with Different Types of Backpacks in Cycling. T2 - ISEA 2020 Online - Proceedings 2020, 49(1) KW - Sports Equipment Technologies KW - Cycling Y1 - 2020 SP - 86 EP - 86 ER - TY - GEN A1 - Rohatsch, Lukas T1 - Open Data Workshop KW - Open Data KW - Open Government Data Y1 - 2020 ER - TY - JOUR A1 - Sauermann, Stefan ED - RDA-COVID19-Epidemiology WG., Research Data Alliance T1 - Preservation of Individuals’ Privacy in Shared COVID-19 Related Data JF - COVID-19 Data sharing in epidemiology, version 0.054. N2 - This paper provides insight into how restricted data can be incorporated in an open-be-default-by-design digital infrastructure for scientific data. We focus, in particular, on the ethical component of FAIRER (Findable, Accessible, Interoperable, Ethical, and Reproducible) data, and the pseudo-anonymization and anonymization of COVID-19 datasets to protect personally identifiable information (PII). First we consider the need for the customisation of the existing privacy preservation techniques in the context of rapid production, integration, sharing and analysis of COVID-19 data. Second, the methods for the pseudo-anonymization of direct identification variables are discussed. We also discuss different pseudo-IDs of the same person for multi-domain and multi-organization. Essentially, pseudo-anonymization and its encrypted domain specific IDs are used to successfully match data later, if required and permitted, as well as to restore the true ID (and authenticity) in individual cases of a patient's clarification.Third, we discuss application of statistical disclosure control (SDC) techniques to COVID-19 disease data. To assess and limit the risk of re-identification of individual persons in COVID-19 datasets (that are often enriched with other covariates like age, gender, nationality, etc.) to acceptable levels, the risk of successful re-identification by a combination of attribute values must be assessed and controlled. This is done using statistical disclosure control for anonymization of data. Lastly, we discuss the limitations of the proposed techniques and provide general guidelines on using disclosure risks to decide on appropriate modes for data sharing to preserve the privacy of the individuals in the datasets. KW - COVID19 KW - pseudo-anonymization KW - statistical disclosure control KW - data sharing KW - privacy Y1 - 2020 ER - TY - CHAP A1 - Orsolits, Horst T1 - Virtual Engineering Applied in a Digital Learning Factory T2 - Perspectives in Industry Digitalization and Advanced Manufacturing in the Black Sea Region KW - Virtual Engineering Y1 - 2021 ER - TY - CHAP A1 - Litzenberger, Stefan T1 - The Influence of Backpack-Design on Thermophysiological Parameters during Simulated Hiking Activities. T2 - ISEA 2020 Online - Proceedings 2020, 49(1) KW - Sports Equipment Technologies KW - Hiking Y1 - 2020 SP - 59 EP - 59 ER - TY - JOUR A1 - Sauermann, Stefan T1 - COVID-19 Questionnaires, Surveys, and Item-Banks: Overview of Clinical- and Population-Based Instruments JF - SSRN N2 - New COVID-19 related instruments are being rapidly developed around the world to collect patient- and population-based information. Heterogeneity between instruments limits comparability of results. The present study provides an overview of instruments and resources. We scoped the content domain on a selection of instruments using the Maelstrom taxonomy. Content of the instruments varied widely, from proximal measures (e.g., clinical symptoms, comorbidities, etc.) to distal measures such as political attitudes. We recommend that researchers reuse existing instruments to the greatest extent possible, and that they make results openly available in machine-readable format to facilitate reuse and maximize comparability of results across studies and countries. KW - COVID19 KW - Questionnaires KW - SARS-COV2 Y1 - 2020 ER - TY - GEN A1 - Sauermann, Stefan T1 - FSR Online Debate: Facilitating interoperability of energy services in Europe – (what) can we learn from existing experience?” KW - Interoperability KW - eHealth Y1 - 2020 ER - TY - GEN A1 - Sauermann, Stefan T1 - Die Gazelle in der Krankenhaus-IT – neue Wege für die Sicherheitstechnische Kontrolle und Leistungsüberprüfung KW - Krankenhaus IT Y1 - 2020 ER - TY - GEN A1 - Sauermann, Stefan T1 - Presentation of successful use of IHE profiles in national strategies (Austria and Switzerland) KW - eHealth Y1 - 2020 ER - TY - GEN A1 - Sauermann, Stefan T1 - Interoperabilität von Prozessen und IT-Systemen im Gesundheitswesen KW - QM und Digitalisierung KW - Interoperabilität Y1 - 2020 ER - TY - GEN A1 - Sauermann, Stefan T1 - Forum Spital für Manager 2019 - Digitalisierung im Krankenhaus - Vorsitzender KW - eHealth KW - Digitalisierung im Krankenhaus Y1 - 2020 ER - TY - GEN A1 - Sauermann, Stefan T1 - Data and Integrated Health Care: � Potential and Challenges of IT innovation KW - eHealth Y1 - 2020 ER - TY - GEN A1 - Sauermann, Stefan T1 - The potential of multi-stakeholder alignment in diabetes KW - Diabetes Y1 - 2020 ER - TY - GEN A1 - Sauermann, Stefan T1 - IHE Education & IHE Internationales KW - Health Interoperability KW - eHealth Y1 - 2020 ER - TY - CHAP A1 - Waller, Daniela T1 - Did COVID19 improve our teaching? T2 - ICERI - 13th annual International Conference of Education, Research and Innovation KW - Covid19 KW - teaching KW - eLearning KW - Distance Learning KW - didactic Y1 - ER - TY - GEN A1 - Sauermann, Stefan T1 - Software –eine Aufgabe für den TSB?Orientierung im Dschungel der Beauftragten! KW - eHealth KW - Krankenhaus-IT Y1 - 2020 ER - TY - GEN A1 - Rohatsch, Lukas T1 - Open Data Projects KW - Open Data KW - Open Government Data KW - Smart City Wien KW - Datenanalyse KW - Softwareentwicklung Y1 - ER - TY - JOUR A1 - Sauermann, Stefan T1 - RDA COVID-19 Working Group. Recommendations and Guidelines on data sharing JF - Research Data Alliance, 2020. N2 - The Research Data Alliance (RDA) COVID-19 Working Group members bring various, global expertise to develop a body of work that comprises how data from multiple disciplines inform response to a pandemic combined with guidelines and recommendations on data sharing under the present COVID-19 cicumstances. This extends to research software sharing, in recognition of the key role in software in analysing data. The work has been divided into four research areas (namely, clinical, omics, epidemiology, social sciences) with four cross cutting themes (namely, community participation, indigenous data, legal and ethical considerations, research software), as a way to focus the conversations, and provide an initial set of guidelines in a tight timeframe. The detailed guidelines are aimed to help stakeholders follow best practices to maximise the efficiency of their work, and to act as a blueprint for future emergencies. The recommendations in the document are aimed at helping policymakers and funders to maximise timely, quality data sharing and appropriate responses in such health emergencies. This work was executed in an intense period over just over 6 weeks, with five iterations, all of which were opened for public community comment. Draft releases and comments are avaialable here (https://doi.org/10.15497/rda00046). This activity has been conducted under the RDA guiding principles of Openness, Consensus, Balance, Harmonization, Community-driven, and Non-profit and technology-neutral. KW - COVID19 Data Sharing Y1 - 2020 ER - TY - BOOK A1 - Orsolits, Horst A1 - Lackner, Maximilian T1 - Virtual Reality und Augmented Reality in der Digitalen Produktion KW - Virtual Reality Y1 - 2021 SN - 978-3-658-29008-5 PB - Gabler ER - TY - RPRT A1 - Pasteka, Richard T1 - Der Standard, "Eine Lunge, die Beatmungsgeräte testet", 2019, Vienna, Austria. KW - Lungensimulation KW - Biomedical Engineering Y1 - 2020 ER - TY - BOOK A1 - Pasteka, Richard A1 - Hrušková, Jana A1 - Jakubík, Juraj A1 - Hendrych, Michal A1 - Svačinová, Jana A1 - Nováková, Zuzana A1 - Vejtasová, Veronika A1 - Kujalová, Veronika A1 - Budinskaya, Ksenia T1 - Fyziologie – teorie k praktickým cvičením T1 - Physiologie - Theorie zu praktischen Übungen KW - Physiology Y1 - N1 - Erstellt in Zusammenarbeit mit dem Servicezentrum für e-Learning der Masarykova univerzita PB - Masarykova univerzita, Fakulta informatiky Masarykovy univerzity CY - Brno ER - TY - GEN A1 - Pasteka, Richard T1 - International Lecture, University of Trás-os-Montes and Alto Douro, Portugal, "Human Respiratory System Modelling" KW - Biomedical Engineering Y1 - 2020 ER - TY - BOOK A1 - Hrušková, Jana A1 - Pasteka, Richard A1 - Jakubík, Juraj A1 - Hendrych, Michal A1 - Svačinová, Jana A1 - Nováková, Zuzana A1 - Vejtasová, Veronika A1 - Kujalová, Veronika A1 - Budinskaya, Ksenia T1 - Workbook fyziologie protokoly BT - Biomedicínská techniky a nelékařské obory KW - Physiology Y1 - SN - 978-80-210-9897-8 PB - Masarykova univerzita CY - Brno ER - TY - CHAP A1 - Pasteka, Richard A1 - Forjan, Mathias T1 - Actively breathing mechanical lung simulator development and preliminary measurements T2 - IFMBE,volume 65; EMBEC & NBC 2017 KW - Biomedical Engineering KW - mechanical lung-simulator Y1 - ER - TY - JOUR A1 - Pasteka, Richard A1 - Forjan, Mathias A1 - Sauermann, Stefan A1 - Drauschke, Andreas T1 - Electro-mechanical Lung Simulator Using Polymer and Organic Human Lung Equivalents for Realistic Breathing Simulation JF - Scientific Reports N2 - Simulation models in respiratory research are increasingly used for medical product development and testing, especially because in-vivo models are coupled with a high degree of complexity and ethical concerns. This work introduces a respiratory simulation system, which is bridging the gap between the complex, real anatomical environment and the safe, cost-effective simulation methods. The presented electro-mechanical lung simulator, xPULM, combines in-silico, ex-vivo and mechanical respiratory approaches by realistically replicating an actively breathing human lung. The reproducibility of sinusoidal breathing simulations with xPULM was verified for selected breathing frequencies (10–18 bpm) and tidal volumes (400–600 ml) physiologically occurring during human breathing at rest. Human lung anatomy was modelled using latex bags and primed porcine lungs. High reproducibility of flow and pressure characteristics was shown by evaluating breathing cycles (nTotal = 3273) with highest standard deviation |3σ| for both, simplified lung equivalents (μV˙ = 23.98 ± 1.04 l/min, μP = −0.78 ± 0.63 hPa) and primed porcine lungs (μV˙ = 18.87 ± 2.49 l/min, μP = −21.13 ± 1.47 hPa). The adaptability of the breathing simulation parameters, coupled with the use of porcine lungs salvaged from a slaughterhouse process, represents an advancement towards anatomically and physiologically realistic modelling of human respiration. KW - Breathing Simulation KW - Lung Simulator KW - Biomedical Engineering Y1 - 2020 VL - Vol 9 IS - No. 1 SP - Article number: 19778 ER - TY - GEN A1 - Pasteka, Richard T1 - International Week Turku, "Hands-on learning and research applications" KW - Biomedical Engineering Y1 - 2020 ER - TY - CHAP A1 - Pasteka, Richard A1 - Santos da Costa, Joao Pedro A1 - Forjan, Mathias T1 - Characteristic Waveforms for Testing of Medical Aerosol Inhalers T2 - 8th European Medical and Biological Engineering Conference N2 - Respiratory diseases are characterised by high prevalence among the European population. Medical aerosol inhalers are the most commonly used means of drug delivery into the human respiratory system. This paper focuses on characteristic waveforms that can be utilised during aerosol deposition studies to simulate conditions of rapid human inhalation. Additionally, an inhalatory waveform based on clinically recorded spirometry data is introduced. Experimental measurements are performed and simulation results mutually compared using the electro-mechanical lung simulator xPULM. The inhalatory waveforms are repeatably simulated with high fidelity in regards to the waveform shape with the lowest value of the Goodness of fit 0.89. Additionally, the measured values for all characteristic inhalatory parameters are simulated with low standard deviation < 1. The differences between the required and measured waveform shapes are small, < 3 L/min and do not influence the overall inhalatory volume. This opens a possibility of utilising the xPULM for medical aerosol inhalers testing. KW - Breathing Simulation KW - Biomedical Engineering Y1 - SP - 240 EP - 246 PB - Springer International Publishing ER - TY - CHAP A1 - Pasteka, Richard A1 - Forjan, Mathias T1 - Changes of particle deposition caused by different breathing patterns during active lung simulation T2 - 41st Annual International Conference of the IEEE Engineering in Medicine and Biology Society (EMBC) N2 - Aerosols are an integral part of everyday life and as such are inhaled under various conditions and circumstances. These may vary based on the health and activity status of an individual. The aim of this work is to analyse the particle deposition mechanisms during the simulation of three different breathing patterns using an aerosol representing the PM1 fraction of fine particles. The active electro-mechanical lung simulator xPULM is utilized as a driving force and is combined with a non-invasive direct reading optical aerosol measurement system. Results show differences between the number of deposited particles for the three breathing patterns and for the three typical size ranges of airborne particles. Overall, the presented approach demonstrates the possibility of determining the changes of aerosol uptake based on different breathing patterns using the electro-mechanical lung simulator and laboratory produced aerosols. Further measurement cycles must be performed in order to validate the found interactions and to characterize the major influencing parameters. KW - Biomedical Engineering, Breathing simulation Y1 - ER - TY - GEN A1 - Pasteka, Richard A1 - Forjan, Mathias A1 - Drauschke, Andreas T1 - Comparison of breathing patterns for aerosol inhalation using an electro-mechanical lung simulator T2 - ALTEX Proceedings - EUSAAT 2018, Linz KW - Biomedical Engineering KW - mechanical lung-simulator Y1 - 2018 ER - TY - GEN A1 - Forjan, Mathias A1 - Pasteka, Richard A1 - Drauschke, Andreas T1 - Lung simulation – an alterna� tive approach to animal testing for applications in aerosol and respiratory research T2 - ALTEX Proceedings - EUSAAT 2018, Linz KW - Biomedical Engineering KW - lung simulation Y1 - ER - TY - CHAP A1 - Pasteka, Richard A1 - Forjan, Mathias A1 - Sauermann, Stefan T1 - Development of a multidisciplinary and telemedicine focused system database T2 - Health Informatics meets eHealth. Digital Insight – Information-Driven Health & Care Proceedings of the 11th eHealth2017 Conference (Studies in Health Technology and Informatics, Volume 236) N2 - Tele-rehabilitation at home is one of the promising approaches in increasing rehabilitative success and simultaneously decreasing the financial burden on the healthcare system. Objectives: Novel and mostly mobile devices are already in use, but shall be used in the future to a higher extent for allowing at home rehabilitation processes at a high quality level. The combination of exercises, assessments and available equipment is the basic objective of the presented database. Methods: The database has been structured in order to allow easy-to-use and fast access for the three main user groups. Therapists – looking for exercise and equipment combinations – patients – rechecking their tasks for home exercises – and manufacturers – entering their equipment for specific use cases. Results: The database has been evaluated by a proof of concept study and shows a high degree of applicability for the field of rehabilitative medicine. Currently it contains 110 exercises/assessments and 111 equipment/systems. Conclusion: Foundations of presented database are already established in the rehabilitative field of application, but can and will be enhanced in its functionality to be usable for a higher variety of medical fields and specificatios. KW - telemedicine KW - e-Health Y1 - SP - 144 EP - 151 PB - IOS Press eBooks ER - TY - GEN A1 - Pasteka, Richard T1 - Applications of Biomedical Engineering in Respiratory Care KW - Biomedical Engineering Y1 - ER - TY - CHAP A1 - Pasteka, Richard A1 - Forjan, Mathias T1 - Changes of particle deposition caused by different breathing patterns during active lung simulation T2 - 41st Annual International Conference of the IEEE Engineering in Medicine and Biology Society (EMBC), 2019 KW - Lung Simulation KW - Breathing Simulation KW - Biomedical Engineering Y1 - 2020 SP - 4969 EP - 4972 ER - TY - CHAP A1 - Himmelstoß, Felix A1 - Iz, Ali A1 - Votzi, Helmut T1 - Quadratic bidirectional buck-boost converter T2 - Proceedings of 2020 International Symposium on Power Electronics, Electrical Drives, Automation and Motion (SPEEDAM), Sorrento, Italy, June 24-26, 2020 KW - Buck-Boost converter KW - Quadratic KW - Bidirectional Y1 - 2020 SP - 738 EP - 743 ER - TY - JOUR A1 - Himmelstoß, Felix A1 - Votzi, Helmut T1 - Feedforward Control of Quadratic Converters JF - WSEAS Transactions of Circuits and Systems KW - DC/DC converters KW - quadratic KW - feedforward control KW - step-down Y1 - VL - 20 IS - 2021 SP - 147 EP - 155 ER - TY - CHAP A1 - Himmelstoß, Felix A1 - Votzi, Helmut T1 - Four quadratic DC/DC converters with one low-side switch and a tapped inductor at the input side T2 - Proceedings of IECON 2019, 45th Annual Conference of the IEEE Industrial Electronics Society, Lisbon, Portugal, Sep. 14-17, Lisbon Portugal KW - DC/DC Converter KW - Quadratic KW - Tapped Inductor Y1 - 2020 SP - 1774 EP - 1779 ER - TY - CHAP A1 - Himmelstoß, Felix A1 - Votzi, Helmut T1 - Simple Inductive Charge Pump T2 - Proceedings of 2020 IEEE 14th International Conference on Compatibility, Power Electronics and Power Engineering (CPE-POWERENG), Setubal, Portugal, 1-3 April, 2020 KW - DC/DC Converter KW - Charge Pump KW - Inductive Y1 - 2020 SP - 291 EP - 296 ER - TY - CHAP A1 - Himmelstoß, Felix A1 - Votzi, Helmut T1 - A Floating Double Buck-Boost Converter as Driver for a Permanent Exited DC Machine T2 - Proceedings of the 2021 International Conference on Electrical Drives & Power Electronics (EDPE 2021), 22.-24. September 2021, Dubrovnik, Croatia KW - DC/DC converter KW - permanent magnet DC machine KW - modeling Y1 - SP - 72 EP - 77 ER - TY - JOUR A1 - Himmelstoß, Felix A1 - Edelmoser, Karl T1 - Flyback Converter with Quadratic Pre-Stage JF - WSEAS Transactions of Circuits and Systems, Vol. 19, 2020 KW - DC/DC Converter KW - Flyback Converter KW - Quadratic KW - Modeling Y1 - 2020 VL - 2020 IS - Vol. 19 SP - 156 EP - 163 ER - TY - CHAP A1 - Himmelstoß, Felix A1 - Votzi, Helmut T1 - Converters to drive two brushed DC machines T2 - 2021 IEEE 15th International Conference on Compatibility, Power Electronics and Power Engineering (CPE-POWERENG), 14-16 July 2021, Florence, Italy KW - DC converters KW - signal flow graph KW - step-up-down KW - permanent magnet (PM) DC machine Y1 - ER - TY - CHAP A1 - Himmelstoß, Felix A1 - Poepperl, Florian T1 - Inverting Zero-Current-Switching Buck Converter T2 - 2021 IEEE 19th International Power Electronics and Motion Control Conference (PEMC), 26.-29. April 2021, Glivice, Poland KW - DC/DC converter KW - zero current switching KW - resonant converter Y1 - SP - 108 EP - 113 ER - TY - JOUR A1 - Himmelstoß, Felix A1 - Edelmoser, Karl T1 - Modified Fourth-Order Buck-Boost Converter JF - WSEAS Transactions of Circuits and Systems KW - buck-boost converter KW - constant input current KW - large signal model KW - small signal model KW - simulation Y1 - SP - 33 EP - 40 ER - TY - CHAP A1 - Himmelstoß, Felix A1 - Edelmoser, Karl T1 - Modified Basic Converters in the Discontinuous Mode T2 - 2021 IEEE 19th International Power Electronics and Motion Control Conference (PEMC), 26.-29. April 2021, Glivice, Poland KW - buck-boost converter KW - discontinuous mode KW - ringing cancelation Y1 - SP - 118 EP - 123 ER - TY - JOUR A1 - Himmelstoß, Felix A1 - Votzi, Helmut T1 - Converter for the Drive of two Brushed DC motors JF - WSEAS Transactions of Circuits and Systems KW - brushed DC machine KW - PMDC motor KW - DC/DC converter KW - three-port converter Y1 - VL - 15 IS - 2020 SP - 249 EP - 256 ER - TY - CHAP A1 - Himmelstoß, Felix A1 - Votzi, Helmut A1 - Windisch, Michael T1 - Quadratic DC/DC converter with autotransformer at the output side T2 - Proceedings of 2020 IEEE 29th International Symposium on Industrial Electronics (ISIE), Delft, Netherlands, June 17-19, 2020 KW - DC/DC Converter KW - Quadratic KW - Tapped Inductor Y1 - 2020 SP - 767 EP - 772 ER - TY - JOUR A1 - Yalcin, Faruk A1 - Himmelstoß, Felix T1 - Boost Converter based 3-phase AC-AC Active Tracking Voltage Regulator Controlled by a Robust Hybrid Control Method JF - Sakarya University Journal of Science KW - AC-AC regulator KW - active tracking KW - boost converter KW - three-phase KW - THD Y1 - VL - 2022 IS - 26(1) SP - 24 EP - 37 ER - TY - CHAP A1 - Himmelstoß, Felix A1 - Jungmayer, Michael T1 - Zero-Current-Switching Buck Converter T2 - Proceedings of 2019 International Conference on Electrical Drives & Power Electronics (EDPE), Novy Smokovec, Slovakia, 24-26 Sept. 2019 KW - DC/DC Converter KW - Zero Current Switching KW - Resonance Converter Y1 - 2020 SP - 36 EP - 41 ER - TY - JOUR A1 - Himmelstoß, Felix A1 - Edelmoser, Karl T1 - Modified Buck-Boost Converter as DC Motor Driver with Continuous Input Current JF - WSEAS Transactions of Circuits and Systems KW - DC/DC converter KW - DC motor drive KW - modified Buck-Boost KW - large and small signal model Y1 - VL - 2020 IS - 19 SP - 186 EP - 194 ER - TY - JOUR A1 - Schneider, Jaana A1 - Pultar, Marianne A1 - Oesterreicher, Johannes A1 - Bobbili, Madhusudhan Reddy A1 - Mühleder, Severin A1 - Priglinger, Eleni A1 - Redl, Heinz A1 - Spittler, Andreas A1 - Grillari, Johannes A1 - Holnthoner, Wolfgang T1 - Cre mRNA Is Not Transferred by EVs from Endothelial and Adipose-Derived Stromal/Stem Cells during Vascular Network Formation JF - Int J Mol Sci. N2 - Coculture systems employing adipose tissue-derived mesenchymal stromal/stem cells (ASC) and endothelial cells (EC) represent a widely used technique to model vascularization. Within this system, cell-cell communication is crucial for the achievement of functional vascular network formation. Extracellular vesicles (EVs) have recently emerged as key players in cell communication by transferring bioactive molecules between cells. In this study we aimed to address the role of EVs in ASC/EC cocultures by discriminating between cells, which have received functional EV cargo from cells that have not. Therefore, we employed the Cre-loxP system, which is based on donor cells expressing the Cre recombinase, whose mRNA was previously shown to be packaged into EVs and reporter cells containing a construct of floxed dsRed upstream of the eGFP coding sequence. The evaluation of Cre induced color switch in the reporter system via EVs indicated that there is no EV-mediated RNA transmission either between EC themselves or EC and ASC. However, since Cre mRNA was not found present in EVs, it remains unclear if Cre mRNA is generally not packaged into EVs or if EVs are not taken up by the utilized cell types. Our data indicate that this technique may not be applicable to evaluate EV-mediated cell-to-cell communication in an in vitro setting using EC and ASC. Further investigations will require a functional system showing efficient and specific loading of Cre mRNA or protein into EVs. KW - Tissue Engineering KW - Stem Cells KW - Vascular Network Formation KW - EVs KW - Endothelial Cells Y1 - VL - 2021 IS - 22(8) SP - 4050 ER - TY - CHAP A1 - Widhalm, Dominik A1 - Goeschka, Karl M. A1 - Kastner, Wolfgang T1 - Undervolting on wireless sensor nodes: a critical perspective T2 - Proceedings of the 23rd International Conference on Distributed Computing and Networking (ICDCN 2022), 4-7 January 2022, Delhi, India KW - wireless sensor network KW - wireless sensor node KW - undervolting KW - energy efficiency KW - faults Y1 - U6 - http://dx.doi.org/https://doi.org/10.1145/3491003.3491018 ER - TY - JOUR A1 - Schneider, Karl H. A1 - Rohringer, Sabrina A1 - Kapeller, Barbara A1 - Grasl, Christian A1 - Kiss, Herbert A1 - Heber, Stefan A1 - Walter, Ingrid A1 - Teuschl, Andreas H. A1 - Podesser, Bruno K. A1 - Bergmeister, Helga T1 - Riboflavin-mediated photooxidation to improve the characteristics of decellularized human arterial small diameter vascular grafts JF - Acta Biomaterialia N2 - Vascular grafts with a diameter of less than 6 mm are made from a variety of materials and techniques to provide alternatives to autologous vascular grafts. Decellularized materials have been proposed as a possible approach to create extracellular matrix (ECM) vascular prostheses as they are naturally derived and inherently support various cell functions. However, these desirable graft characteristics may be limited by alterations of the ECM during the decellularization process leading to decreased biomechanical properties and hemocompatibility. In this study, arteries from the human placenta chorion were decellularized using two distinct detergents (Triton X-100 or SDS), which differently affect ECM ultrastructure. To overcome biomechanical strength loss and collagen fiber exposure after decellularization, riboflavin-mediated UV (RUV) crosslinking was used to uniformly crosslink the collagenous ECM of the grafts. Graft characteristics and biocompatibility with and without RUV crosslinking were studied in vitro and in vivo. RUV-crosslinked ECM grafts showed significantly improved mechanical strength and smoothening of the luminal graft surfaces. Cell seeding using human endothelial cells revealed no cytotoxic effects of the RUV treatment. Short-term aortic implants in rats showed cell migration and differentiation of host cells. Functional graft remodeling was evident in all grafts. Thus, RUV crosslinking is a preferable tool to improve graft characteristics of decellularized matrix conduits. KW - Tissue Engineering KW - Small diameter vascular graft KW - Biomaterials KW - Decellularization KW - Surface modification Y1 - VL - 116 SP - 246 EP - 258 ER - TY - CHAP A1 - Himmelstoß, Felix A1 - Edelmoser, Karl T1 - Quadratic Flyback Converter T2 - Proceedings of PCIM Europe digital days 2020; International Exhibition and Conference for Power Electronics, Intelligent Motion, Renewable Energy and Energy Management, Nuernberg, Germany, 7-8 July, 2020 KW - Quadratic Power Converter KW - Flyback Converter KW - Modeling Y1 - 2020 ER - TY - JOUR A1 - Holnthoner, Wolfgang T1 - Adipose-tissue-derived therapeutic cells in their natural environment as an autologous cell therapy strategy: the microtissue-stromal vascular fraction JF - Eur Cell Mater. N2 - The prerequisite for a successful clinical use of autologous adipose-tissue-derived cells is the highest possible regenerative potential of the applied cell population, the stromal vascular fraction (SVF). Current isolation methods depend on high enzyme concentration, lysis buffer, long incubation steps and mechanical stress, resulting in single cell dissociation. The aim of the study was to limit cell manipulation and obtain a derivative comprising therapeutic cells (microtissue-SVF) without dissociation from their natural extracellular matrix, by employing a gentle good manufacturing practice (GMP)-grade isolation. The microtissue-SVF yielded larger numbers of viable cells as compared to the improved standard-SVF, both with low enzyme concentration and minimal dead cell content. It comprised stromal tissue compounds (collagen, glycosaminoglycans, fibroblasts), capillaries and vessel structures (CD31+, smooth muscle actin+). A broad range of cell types was identified by surface-marker characterisation, including mesenchymal, haematopoietic, pericytic, blood and lymphatic vascular and epithelial cells. Subpopulations such as supra-adventitial adipose-derived stromal/stem cells and endothelial progenitor cells were significantly more abundant in the microtissue-SVF, corroborated by significantly higher potency for angiogenic tube-like structure formation in vitro. The microtissue-SVF showed the characteristic phenotype and tri-lineage mesenchymal differentiation potential in vitro and an immunomodulatory and pro-angiogenic secretome. In vivo implantation of the microtissue-SVF combined with fat demonstrated successful graft integration in nude mice. The present study demonstrated a fast and gentle isolation by minor manipulation of liposuction material, achieving a therapeutically relevant cell population with high vascularisation potential and immunomodulatory properties still embedded in a fraction of its original matrix. KW - Adipose-tissue KW - autologous cell therapy KW - microtissue KW - stromal vascular fraction KW - vascularization Y1 - 2020 VL - 2019 IS - Feb, 37 SP - 113 EP - 133 ER - TY - CHAP A1 - Widhalm, Dominik A1 - Goeschka, Karl M. A1 - Kastner, Wolfgang T1 - Node-level indicators of soft faults in wireless sensor networks T2 - Proceedings of the 40th International Symposium on Reliable Distributed Systems (SRDS 2021), 20-23 September 2021, Chicago, USA KW - wireless sensor network KW - wireless sensor node KW - soft faults KW - fault tolerance Y1 - SP - 13 EP - 22 PB - IEEE ER - TY - JOUR A1 - Himmelstoß, Felix T1 - Improved positive output voltage super-lift Boost converter JF - WSEAS TRANSACTIONS on POWER SYSTEMS KW - DC/DC converter KW - feedforward control KW - boost converter KW - simulation Y1 - VL - 2022 IS - Vol. 17 SP - 68 EP - 75 ER - TY - JOUR A1 - Strohmeier, Karin A1 - Hofmann, Martina A1 - Jacak, Jaroslaw A1 - Narzt, Marie-Sophie A1 - Wahlmueller, Marlene A1 - Mairhofer, Mario A1 - Schädl, Barbara A1 - Holnthoner, Wolfgang A1 - Barsch, Martin A1 - Sandhofer, Matthias A1 - Wolbank, Susanne A1 - Priglinger, Eleni T1 - Multi-Level Analysis of Adipose Tissue Reveals the Relevance of Perivascular Subpopulations and an Increased Endothelial Permeability in Early-Stage Lipedema JF - Biomedicines N2 - Lipedema is a chronic, progressive disease of adipose tissue with unknown etiology. Based on the relevance of the stromal vascular fraction (SVF) cell population in lipedema, we performed a thorough characterization of subcutaneous adipose tissue, SVF isolated thereof and the sorted populations of endothelial cells (EC), pericytes and cultured adipose-derived stromal/stem cells (ASC) of early-stage lipedema patients. We employed histological and gene expression analysis and investigated the endothelial barrier by immunofluorescence and analysis of endothelial permeability in vitro. Although there were no significant differences in histological stainings, we found altered gene expression of factors relevant for local estrogen metabolism (aromatase), preadipocyte commitment (ZNF423) and immune cell infiltration (CD11c) in lipedema on the tissue level, as well as in distinct cellular subpopulations. Machine learning analysis of immunofluorescence images of CD31 and ZO-1 revealed a morphological difference in the cellular junctions of EC cultures derived from healthy and lipedema individuals. Furthermore, the secretome of lipedema-derived SVF cells was sufficient to significantly increase leakiness of healthy human primary EC, which was also reflected by decreased mRNA expression of VE-cadherin. Here, we showed for the first time that the secretome of SVF cells creates an environment that triggers endothelial barrier dysfunction in early-stage lipedema. Moreover, since alterations in gene expression were detected on the cellular and/or tissue level, the choice of sample material is of high importance in elucidating this complex disease. KW - Tissue Engineering KW - Adipose Tissue KW - Lipedema KW - Endothelial Cells Y1 - VL - 2022 IS - 10(5) SP - 1163 ER - TY - CHAP A1 - Himmelstoß, Felix A1 - Edelmoser, Karl T1 - Zero-Current-Switching Boost Converter, Power Conversion and Intelligent Motion T2 - Proceedings of PCIM 2019, Nuernberg, Germany, May 7-9, 2019 KW - Discontinuous Conduction Mode KW - DC/DC Converter KW - Resonant Converter KW - ZCS KW - DCM Y1 - 2019 SN - 978-3-8007-4938-6 SP - 1686 EP - 1691 ER - TY - JOUR A1 - Trisko, Johanna A1 - Fleck, Johanna A1 - Kau, Silvio A1 - Oesterreicher, Johannes A1 - Holnthoner, Wolfgang T1 - Lymphatic and Blood Endothelial Extracellular Vesicles: A Story Yet to Be Written JF - Life N2 - Extracellular vesicles (EVs), such as exosomes, microvesicles, and apoptotic bodies, are cell-derived, lipid bilayer-enclosed particles mediating intercellular communication and are therefore vital for transmitting a plethora of biological signals. The vascular endothelium substantially contributes to the circulating particulate secretome, targeting important signaling pathways that affect blood cells and regulate adaptation and plasticity of endothelial cells in a paracrine manner. Different molecular signatures and functional properties of endothelial cells reflect their heterogeneity among different vascular beds and drive current research to understand varying physiological and pathological effects of blood and lymphatic endothelial EVs. Endothelial EVs have been linked to the development and progression of various vascular diseases, thus having the potential to serve as biomarkers and clinical treatment targets. This review aims to provide a brief overview of the human vasculature, the biology of extracellular vesicles, and the current knowledge of endothelium-derived EVs, including their potential role as biomarkers in disease development. KW - Tissue Engineering KW - Endothelial Cells KW - vascularization KW - extracellular vesicles KW - lymphatic endothelial cells Y1 - VL - 2022 IS - 12(5) SP - 654 ER - TY - JOUR A1 - Sayer, Simon A1 - Zandrini, Tommaso A1 - Markovic, Marica A1 - Van Hoorick, Jasper A1 - Van Vlierberghe, Sandra A1 - Baudis, Stefan A1 - Holnthoner, Wolfgang A1 - Ovsianikov, Aleksandr T1 - Guiding cell migration in 3D with high-resolution photografting JF - Scientific Reports N2 - Multi-photon lithography (MPL) has proven to be a suitable tool to precisely control the microenvironment of cells in terms of the biochemical and biophysical properties of the hydrogel matrix. In this work, we present a novel method, based on multi-photon photografting of 4,4′-diazido-2,2′-stilbenedisulfonic acid (DSSA), and its capabilities to induce cell alignment, directional cell migration and endothelial sprouting in a gelatin-based hydrogel matrix. DSSA-photografting allows for the fabrication of complex patterns at a high-resolution and is a biocompatible, universally applicable and straightforward process that is comparably fast. We have demonstrated the preferential orientation of human adipose-derived stem cells (hASCs) in response to a photografted pattern. Co-culture spheroids of hASCs and human umbilical vein endothelial cells (HUVECs) have been utilized to study the directional migration of hASCs into the modified regions. Subsequently, we have highlighted the dependence of endothelial sprouting on the presence of hASCs and demonstrated the potential of photografting to control the direction of the sprouts. MPL-induced DSSA-photografting has been established as a promising method to selectively alter the microenvironment of cells. KW - Tissue Engineering KW - Cell migration KW - photografting Y1 - VL - 2022 IS - 12(1), 10196 SP - 8626 ER - TY - CHAP A1 - Himmelstoß, Felix A1 - Jungmayer, Michael T1 - A Family of Modified Converters with Limited Duty Cycle T2 - Proceedings of the 2021 International Aegean Conference on Electrical Machines and Power Electronics (ACEMP) & 2021 International Conference on Optimization of Electrical and Electronic Equipment (OPTIM), 2.-3. September 2021, Brasov, Rumania KW - modified converters KW - DC/DC converters KW - AC/DC converters Y1 - U6 - http://dx.doi.org/https://doi.org/10.1109/OPTIM-ACEMP50812.2021.9590064 SP - 246 EP - 253 PB - IEEE ER - TY - JOUR A1 - Widhalm, Dominik A1 - Goeschka, Karl M. A1 - Kastner, Wolfgang T1 - An open-source wireless sensor node platform with active node-level reliability for monitoring applications JF - Sensors KW - sensor node KW - fault diagnosis KW - energy-efficiency KW - wireless sensor network Y1 - VL - 2021 IS - Volume 21, Issue 22, Article-No. 7613 ER - TY - JOUR A1 - Himmelstoß, Felix T1 - Virtual Laboratory: Switching of A Nearly Resistive Load JF - International Journal of Education and Learning Systems KW - virtual laboratory KW - inductive load KW - parasitic inductance KW - switching KW - overvoltage Y1 - VL - 2021 IS - Vol. 6 SP - 13 EP - 19 ER - TY - JOUR A1 - Yalcin, Faruk A1 - Himmelstoß, Felix T1 - Buck-type Single-phase AC-AC Active Tracking Voltage Regulator Controlled by an Enhanced Hybrid Control Method JF - Sakarya University Journal of Science KW - AC-AC regulator KW - active tracking KW - buck converter Y1 - VL - 2021 IS - 25 SP - 926 EP - 937 ER - TY - THES A1 - Widhalm, Dominik T1 - Sensor Node Fault Detection in Wireless Sensor Networks: An Immune-inspired Approach KW - fault detection KW - sensor node KW - node-level diagnostics KW - wireless sensor network KW - anomaly detection Y1 - ER - TY - CHAP A1 - Widhalm, Dominik A1 - Goeschka, Karl M. A1 - Kastner, Wolfgang T1 - Is Arduino a suitable platform for sensor nodes? T2 - Proceedings of the 47th Annual Conference of the IEEE Industrial Electronics Society (IECON 2021), 13-16 October 2021, Toronto, Canada KW - Arduino KW - wireless sensor network KW - sensor node KW - energy consumption Y1 - U6 - http://dx.doi.org/doi: 10.1109/IECON48115.2021.9589479 PB - IEEE ER - TY - JOUR A1 - Himmelstoß, Felix A1 - Edelmoser, Karl T1 - Virtual three-phase laboratory exercise during pandemic situation JF - WSEAS TRANSACTIONS on ADVANCES in ENGINEERING EDUCATION KW - virtual laboratory KW - three-phase systems KW - phasor diagram KW - neutral line interruption KW - voltage displacement Y1 - VL - 2022 ER - TY - CHAP A1 - Himmelstoß, Felix A1 - Votzi, Helmut T1 - Modified d(2-d)/(1-d)2-Converter T2 - Proceedings of the Power Conversion and Intelligent Motion Conference (PCIM) 2022, 10-12 May, 2022 Nurenberg, Germany KW - DC/DC converter KW - quadratic converter KW - modeling Y1 - U6 - http://dx.doi.org/doi: 10.30420/565822200 SP - 1446 EP - 1453 ER - TY - CHAP A1 - Himmelstoß, Felix A1 - Votzi, Helmut T1 - A family of quadratic DC/DC converters with one low-side switch and a tapped inductor at the output side T2 - Aegean Conference on Electrical Machines and Power Electronics & Optimization of Electrical & Electronic Equipment Conference, Istanbul Turkey, August, 27 – 29, 2019 KW - DC/DC Converter KW - Tapped Inductor Y1 - 2019 ER - TY - JOUR A1 - Yalcin, Faruk A1 - Himmelstoß, Felix T1 - Single-phase Boost-type Active Tracking AC-AC Voltage Regulator with an Improved Hybrid Control Technique JF - Duzce University Journal of Science and Technology KW - Boost converter KW - AC-AC regulator KW - Single-phase KW - Active tracking Y1 - VL - 2022 IS - 10, issue 1 SP - 139 EP - 153 ER - TY - JOUR A1 - Rothbauer, Mario A1 - Byrne, Ruth A. A1 - Schobesberger, Silvia A1 - Olmos Calvo, Isabel A1 - Fischer, Anita A1 - Reihs, Eva I. A1 - Spitz, Sarah A1 - Bachmann, Barbara A1 - Sevelda, Florian A1 - Holinka, Johannes A1 - Holnthoner, Wolfgang A1 - Redl, Heinz A1 - Toegel, Stefan A1 - Windhager, Reinhard A1 - Kiener, Hans P. A1 - Ertl, Peter T1 - Establishment of a human three-dimensional chip-based chondro-synovial coculture joint model for reciprocal cross talk studies in arthritis research JF - Lab on a Chip N2 - Rheumatoid arthritis is characterised by a progressive, intermittent inflammation at the synovial membrane, which ultimately leads to the destruction of the synovial joint. The synovial membrane as the joint capsule's inner layer is lined with fibroblast-like synoviocytes that are the key player supporting persistent arthritis leading to bone erosion and cartilage destruction. While microfluidic models that model molecular aspects of bone erosion between bone-derived cells and synoviocytes have been established, RA's synovial-chondral axis has not yet been realised using a microfluidic 3D model based on human patient in vitro cultures. Consequently, we established a chip-based three-dimensional tissue coculture model that simulates the reciprocal cross talk between individual synovial and chondral organoids. When co-cultivated with synovial organoids, we could demonstrate that chondral organoids induce a higher degree of cartilage physiology and architecture and show differential cytokine response compared to their respective monocultures highlighting the importance of reciprocal tissue-level cross talk in the modelling of arthritic diseases. KW - Tissue Engineering KW - coculture joint model KW - arthritis KW - human three-dimensional chip Y1 - VL - 2021 IS - 21 SP - 4128 EP - 4143 ER - TY - JOUR A1 - Yalcin, Faruk A1 - Himmelstoß, Felix T1 - Three-phase Active Tracking AC-AC Voltage Regulator based on Buck Converter with an Efficient Hybrid Control Technique JF - Düzce University Journal of Science & Technology KW - buck converter KW - AC-AC regulator KW - active tracking KW - THD Y1 - VL - 2022 IS - 10 SP - 537 EP - 554 ER - TY - CHAP A1 - Himmelstoß, Felix T1 - Controller design of a Buck converter with the help of LTSpice T2 - Proceedings of the International Asian Congress of Contemporary Sciences, 27-29 May, 2022, Van, Turkiye KW - DC/DC converter KW - Buck converter KW - feedforward KW - compensation controller KW - simulation Y1 - SP - 195 EP - 202 ER - TY - GEN A1 - Langer, Sarah A1 - Nemec, Iris A1 - Kollmitzer, Josef A1 - Scherer, Matthias A1 - Martinek, Johannes T1 - Vergleich des decompositionierten und des überlagerten Elektromyogramms zur Analyse der Muskelermüdung N2 - Ziel dieser Studie war das überlagerte Elektromyogramm (ueEMG) und das decompositionierte (dEMG) auf deren Reliabilität und Aussagekraft zu überprüfen. Hierfür vollzogen zehn gesunde männliche Probanden zu drei Testzeitpunkten isometrische Ermüdungsmessungen. Hintergrund Bei der Messung des ueEMG werden die Motor Units Aktionspotentiale (MUAPS) elektrisch überlagert. Physiologische Muskelermüdung zeigt sich hier unter anderem in der Veränderung des Frequenzspektrums und der Signalamplitude. Letzteres steigt bei Ermüdung an, wohingegen die mediane Frequenz (MF) sinkt. Eine weitere Möglichkeit die Muskelermüdung mittels Oberflächen-EMG zu ermitteln ist die Anwendung des dEMGs. Dieses ermöglicht mithilfe einer Mustererkennungssoftware einzelne MUAPS zu erkennen. Studien zeigten, dass aktive Motor Units (MU) bei wiederholten oder anhaltenden submaximalen Kontraktionen als Muskelermüdung ihre Feuerungsrate erhöhen und neue MU rekrutiert werden. Bisher wurden diese Methoden jeweils einzeln betrachtet. Um sicherzustellen welcher Ansatz für klinische Daten geeigneter ist, wurden Datensätze zu beiden Methoden gleichzeitig generiert und hinsichtlich ihrer Aussagekraft und Wiederholbarkeit mit statistischen Mitteln verglichen. KW - Elektromyogramm KW - Rehabilitationstechnik Y1 - 2022 ER -