TY  - JOUR
A1  - Tomasch, Janine
A1  - Maleiner, Babette
A1  - Heher, Philipp
A1  - Rufin, Manuel
A1  - Andriotis, Orestis G.
A1  - Thurner, Philipp J.
A1  - Redl, Heinz
A1  - Fuchs, Christiane
A1  - Teuschl-Woller, Andreas H.
T1  - Changes in Elastic Moduli of Fibrin Hydrogels Within the Myogenic Range Alter Behavior of Murine C2C12 and Human C25 Myoblasts Differently
JF  - Froniers in Bioengineering and Biotechnology
N2  - Fibrin hydrogels have proven highly suitable scaffold materials for skeletal muscle tissue engineering in the past. Certain parameters of those types of scaffolds, however, greatly affect cellular mechanobiology and therefore the myogenic outcome. The aim of this study was to identify the influence of apparent elastic properties of fibrin scaffolds in 2D and 3D on myoblasts and evaluate if those effects differ between murine and human cells. Therefore, myoblasts were cultured on fibrin-coated multiwell plates (“2D”) or embedded in fibrin hydrogels (“3D”) with different elastic moduli. Firstly, we established an almost linear correlation between hydrogels’ fibrinogen concentrations and apparent elastic moduli in the range of 7.5 mg/ml to 30 mg/ml fibrinogen (corresponds to a range of 7.7–30.9 kPa). The effects of fibrin hydrogel elastic modulus on myoblast proliferation changed depending on culture type (2D vs 3D) with an inhibitory effect at higher fibrinogen concentrations in 3D gels and vice versa in 2D. The opposite effect was evident in differentiating myoblasts as shown by gene expression analysis of myogenesis marker genes and altered myotube morphology. Furthermore, culture in a 3D environment slowed down proliferation compared to 2D, with a significantly more pronounced effect on human myoblasts. Differentiation potential was also substantially impaired upon incorporation into 3D gels in human, but not in murine, myoblasts. With this study, we gained further insight in the influence of apparent elastic modulus and culture type on cellular behavior and myogenic outcome of skeletal muscle tissue engineering approaches. Furthermore, the results highlight the need to adapt parameters of 3D culture setups established for murine cells when applied to human cells.
KW  - Tissue Engineering
KW  - Fibrin
KW  - Hydrogel
KW  - Biomaterials
KW  - Cell Culture
Y1  - 
VL  - 10
SP  - 836520
ER  - 
TY  - JOUR
A1  - Eder, Gabriele C.
A1  - Voronko, Yuliya
A1  - Dimitriadis, Simon
A1  - Knöbl, Karl
A1  - Ujvári, Gusztáv
A1  - Berger, Karl A.
A1  - Halwachs, Martin
A1  - Neumaier, Lukas
A1  - Hirschl, Christina
T1  - Climate specific accelerated ageing tests and evaluation of ageing induced electrical, physical, and chemical changes
JF  - Progress in Photovoltaics: Research and Applications 2018
KW  - Photovoltaik
KW  - Degradation
Y1  - 
N1  - DOI:10.1002/pip.3090
SP  - 1
EP  - 16
ER  - 
TY  - CHAP
A1  - Eder, C.
A1  - Voronko, Yuliya
A1  - Dimitriadis, Simon
A1  - Knöbl, Karl
A1  - Ujvári, Gusztáv
A1  - Berger, Karl A.
A1  - Halwachs, Martin
A1  - Neumaier, Lukas
A1  - Hirschl, Christina
T1  - Influence of variable climatic stress conditions on performance and material degradation of PV-modules
T2  - SOPHIA PV-Reliability Workshop 2018, Ljubljana
KW  - Photovoltaics
KW  - Degradation
Y1  - 
ER  - 
TY  - CHAP
A1  - Eder, Gabriele C.
A1  - Voronko, Yuliya
A1  - Dimitriadis, Simon
A1  - Knöbl, Karl
A1  - Ujvári, Gusztáv
A1  - Berger, Karl A.
A1  - Neumaier, Lukas
T1  - Climate Specific Accelerated Ageing Tests & Evaluation of Ageing Induced Electrical, Physical and Chemical Changes
T2  - 35th EU PVSEC 2018 5DO.7.4
KW  - Photovoltaics
KW  - Degradation
Y1  - 
ER  - 
TY  - CHAP
A1  - Eder, Gabriele C.
A1  - Grillberger, Paul
A1  - Voronko, Yuliya
A1  - Kubitschek, Bernhard
A1  - Knöbl, Karl
T1  - UV-Fluorescence measurements as tool for the detection of degradation effects in PV-modules
T2  - Proceedings of the European Weathering Symposium EWS
KW  - Photovoltaics
KW  - Degradation
Y1  - 
ER  - 
TY  - CHAP
A1  - Eder, Gabriele C.
A1  - Voronko, Yuliya
A1  - Kubitschek, Bernhard
A1  - Knöbl, Karl
T1  - Fluorescence Spectroscopy and Imaging on aged polymeric PV Encapsulantes
T2  - 20th European Symposium on Polymer Sprectroscopy 09/2016 Dresden DOI: 10.13140/RG.2.2.20827.36648
KW  - Photovoltaics
KW  - Degradation
Y1  - 
ER  - 
TY  - CHAP
A1  - Eder, Gabriele C.
A1  - Voronko, Yuliya
A1  - Kubitschek, Bernhard
A1  - Knöbl, Karl
T1  - Fluorescence Spectroscopy and Imaging on aged polymeric PV Encapsulantes
T2  - Poster - Österreichische PV-Tagung Villach 11/2016
KW  - Photovoltaics
KW  - Degradation
Y1  - 
ER  - 
TY  - CHAP
A1  - Stohanzl, Martin
A1  - Kubinger, Wilfried
T1  - Low-Cost Multispectral Camera Array with Image Registration
T2  - Proceedings of the Austrian Robotics Workshop 2023, p. 146, 12-13 April, 2023, Linz, Austria
KW  - Multicamera
KW  - Multispectral
KW  - Calibration
Y1  - 
SP  - 146
EP  - 146
ER  -