TY - JOUR A1 - Maleiner, Babette A1 - Tomasch, Janine A1 - Heher, Philipp A1 - Spadiut, Oliver A1 - Rünzler, Dominik A1 - Fuchs, Christiane T1 - The Importance of Biophysical and Biochemical Stimuli in Dynamic Skeletal Muscle Models. JF - Frontiers in Physiology N2 - Classical approaches to engineer skeletal muscle tissue based on current regenerative and surgical procedures still do not meet the desired outcome for patient applications. Besides the evident need to create functional skeletal muscle tissue for the repair of volumetric muscle defects, there is also growing demand for platforms to study muscle-related diseases, such as muscular dystrophies or sarcopenia. Currently, numerous studies exist that have employed a variety of biomaterials, cell types and strategies for maturation of skeletal muscle tissue in 2D and 3D environments. However, researchers are just at the beginning of understanding the impact of different culture settings and their biochemical (growth factors and chemical changes) and biophysical cues (mechanical properties) on myogenesis. With this review we intend to emphasize the need for new in vitro skeletal muscle (disease) models to better recapitulate important structural and functional aspects of muscle development. We highlight the importance of choosing appropriate system components, e.g., cell and biomaterial type, structural and mechanical matrix properties or culture format, and how understanding their interplay will enable researchers to create optimized platforms to investigate myogenesis in healthy and diseased tissue. Thus, we aim to deliver guidelines for experimental designs to allow estimation of the potential influence of the selected skeletal muscle tissue engineering setup on the myogenic outcome prior to their implementation. Moreover, we offer a workflow to facilitate identifying and selecting different analytical tools to demonstrate the successful creation of functional skeletal muscle tissue. Ultimately, a refinement of existing strategies will lead to further progression in understanding important aspects of muscle diseases, muscle aging and muscle regeneration to improve quality of life of patients and enable the establishment of new treatment options. KW - Bioreactor KW - Muscle KW - Biomaterial Y1 - ER - TY - GEN A1 - Heher, Philipp A1 - Tomasch, Janine A1 - Maleiner, Babette A1 - Redl, Heinz A1 - Fuchs, Christiane T1 - The Importance of Biomechanical Cues for In Vitro Skeletal Myogenesis KW - In Vitro KW - Myogenesis Y1 - 2018 ER - TY - CHAP A1 - Meyer, Markus A1 - Donsa, Klaus A1 - Truskaller, Thomas A1 - Frohner, Matthias A1 - Pohn, Birgit A1 - Felfernig, Alexander A1 - Sinner, Franz A1 - Pieber, Thomas T1 - Development of a Protocol for Automated Glucose Measurement Transmission Used in Clinical Decision Support Systems Based on the Continua Design Guidelines T2 - Conference Proceedings eHealth 2018 KW - Mobile Health KW - Standardization KW - Clinical Decision Support Systems KW - Diabetes KW - Medication Errors Y1 - ER - TY - GEN A1 - Meyer, Markus A1 - Donsa, Klaus A1 - Truskaller, Thomas A1 - Frohner, Matthias A1 - Pohn, Birgit A1 - Felfernig, Alexander A1 - Sinner, Franz A1 - Pieber, Thomas T1 - Development of a Protocol for Automated Glucose Measurement Transmission Used in Clinical Decision Support Systems Based on the Continua Design Guidelines KW - Mobile Health KW - Standardization KW - Clinical Decision Support Systems KW - Diabetes KW - Medication Errors Y1 - ER - TY - JOUR A1 - Schanda, Jakob A1 - Keibl, Claudia A1 - Heimel, Patrick A1 - Monforte, Xavier A1 - Feichtinger, Xaver A1 - Teuschl, Andreas A1 - Baierl, Andreas A1 - Muschitz, Christian A1 - Redl, Heinz A1 - Fialka, Christian A1 - Mittermayr, Rainer T1 - Zoledronic Acid Substantially Improves Bone Microarchitecture and Biomechanical Properties After Rotator Cuff Repair in a Rodent Chronic Defect Model JF - Am J Sports Med N2 - Background: Bone mineral density at the humeral head is reduced in patients with chronic rotator cuff tears. Bone loss in the humeral head is associated with repair failure after rotator cuff reconstruction. Bisphosphonates (eg, zoledronic acid) increase bone mineral density. Hypothesis: Zoledronic acid improves bone mineral density of the humeral head and biomechanical properties of the enthesis after reconstruction of chronic rotator cuff tears in rats. Study design: Controlled laboratory study. Methods: A total of 32 male Sprague-Dawley rats underwent unilateral (left) supraspinatus tenotomy with delayed transosseous rotator cuff reconstruction after 3 weeks. All rats were sacrificed 8 weeks after rotator cuff repair. Animals were randomly assigned to 1 of 2 groups. At 1 day after rotator cuff reconstruction, the intervention group was treated with a single subcutaneous dose of zoledronic acid at 100 µg/kg bodyweight, and the control group received 1 mL of subcutaneous saline solution. In 12 animals of each group, micro-computed tomography scans of both shoulders were performed as well as biomechanical testing of the supraspinatus enthesis of both sides. In 4 animals of each group, histological analyses were conducted. Results: In the intervention group, bone volume fraction (bone volume/total volume [BV/TV]) of the operated side was higher at the lateral humeral head (P = .005) and the medial humeral head (P = .010) compared with the control group. Trabecular number on the operated side was higher at the lateral humeral head (P = .004) and the medial humeral head (P = .001) in the intervention group. Maximum load to failure rates on the operated side were higher in the intervention group (P < .001). Cortical thickness positively correlated with higher maximum load to failure rates in the intervention group (r = 0.69; P = .026). Histological assessment revealed increased bone formation in the intervention group. Conclusion: Single-dose therapy of zoledronic acid provided an improvement of bone microarchitecture at the humeral head as well as an increase of maximum load to failure rates after transosseous reconstruction of chronic rotator cuff lesions in rats. Clinical relevance: Zoledronic acid improves bone microarchitecture as well as biomechanical properties after reconstruction of chronic rotator cuff tears in rodents. These results need to be verified in clinical investigations. KW - Tissue Engineering KW - Rotator Cuff Tears Y1 - VL - 2020 Jul IS - 48 (9) SP - 2151 EP - 2160 ER - TY - JOUR A1 - Slezak, Paul A1 - Slezak, Cyrill A1 - Hartinger, Joachim A1 - Teuschl, Andreas A1 - Nürnberger, Sylvia A1 - Redl, Heinz A1 - Mittermayr, Rainer T1 - A Low Cost Implantation Model in the Rat That Allows a Spatial Assessment of Angiogenesis. JF - Frontiers in Bioengineering and Biotechnology N2 - There is continual demand for animal models that allow a quantitative assessment of angiogenic properties of biomaterials, therapies, and pharmaceuticals. In its simplest form, this is done by subcutaneous material implantation and subsequent vessel counting which usually omits spatial data. We have refined an implantation model and paired it with a computational analytic routine which outputs not only vessel count but also vessel density, distribution, and vessel penetration depth, that relies on a centric vessel as a reference point. We have successfully validated our model by characterizing the angiogenic potential of a fibrin matrix in conjunction with recombinant human vascular endothelial growth factor (rhVEGF165). The inferior epigastric vascular pedicles of rats were sheathed with silicone tubes, which were subsequently filled with 0.2 ml of fibrin and different doses of rhVEGF165, centrically embedding the vessels. Over 4 weeks, tissue samples were harvested and subsequently immunohistologically stained and computationally analyzed. The model was able to detect variations over the angiogenic potentials of growth factor spiked fibrin matrices. Adding 20 ng of rhVEGF165 resulted in a significant increase in vasculature while 200 ng of rhVEGF165 did not improve vascular growth. Vascularized tissue volume increased during the first week and vascular density increased during the second week. Total vessel count increased significantly and exhibited a peak after 2 weeks which was followed by a resorption of vasculature by week 4. In summary, a simple implantation model to study in vivo vascularization with only a minimal workload attached was enhanced to include morphologic data of the emerging vascular tree. KW - Tissue Engineering KW - Bioreactor KW - Biomaterial Y1 - ER - TY - JOUR A1 - Berkovitch, Yulia A1 - Cohen, Talia A1 - Peled, Eli A1 - Schmidhammer, Robert A1 - Hildner, Florian A1 - Teuschl, Andreas A1 - Wolbank, Susanne A1 - Yelin, Dvir A1 - Redl, Heinz A1 - Seliktar, Dror T1 - Hydrogel composition and laser micropatterning to regulate sciatic nerve regeneration. JF - Journal of Tissue Engineering and Regenerative Medicine N2 - Treatment of peripheral nerve injuries has evolved over the past several decades to include the use of sophisticated new materials endowed with trophic and topographical cues that are essential for in vivo nerve fibre regeneration. In this research, we explored the use of an advanced design strategy for peripheral nerve repair, using biological and semi-synthetic hydrogels that enable controlled environmental stimuli to regenerate neurons and glial cells in a rat sciatic nerve resection model. The provisional nerve growth conduits were composed of either natural fibrin or adducts of synthetic polyethylene glycol and fibrinogen or gelatin. A photo-patterning technique was further applied to these 3D hydrogel biomaterials, in the form of laser-ablated microchannels, to provide contact guidance for unidirectional growth following sciatic nerve injury. We tested the regeneration capacity of subcritical nerve gap injuries in rats treated with photo-patterned materials and compared these with injuries treated with unpatterned hydrogels, either stiff or compliant. Among the factors tested were shear modulus, biological composition, and micropatterning of the materials. The microchannel guidance patterns, combined with appropriately matched degradation and stiffness properties of the material, proved most essential for the uniform tissue propagation during the nerve regeneration process. KW - Tissue Engineering KW - Biomaterials KW - Nerve Regeneration Y1 - SP - 1049 EP - 1061 ER - TY - JOUR A1 - Nau, Thomas A1 - Teuschl, Andreas A1 - Ebner, Anna A1 - Jung, Ilse A1 - Schenk, Christian T1 - Low revision rate and excellent outcome of primary ACL repair with a minimum follow-up of 5 years. JF - Muscle, ligaments and tendons Journal N2 - Introduction: Due to limitations of ACL reconstruction, primary ACL repair has recently regained research interest. Although abandoned in the past, primary repair with conservation of the original ligament demonstrates considerable advantages compared to reconstruction. We hypothesized that early repair, strictly limited to patients with a proximal ACL rupture and excellent tissue quality of the remaining ACL stump, would lead to equal revision rates and subjective outcomes as reported for ACL reconstruction after a minimum of 5 years. Methods: In this questionnaire study, patients who had a primary ACL repair between 2002 and 2009 were invited to participate. Besides any potential revision surgery, the Tegner activity scale and the Knee Injury and Osteoarthritis Outcome Score were included in the evaluation. Results: Out of 1912 patients who had ACL related surgery during the observation period, 221 (11.4%) had a primary ACL repair. 60 patients (61 knees) were available for follow-up. In 2/61 (3.3%) cases ACL revision surgery was performed and one patient had meniscus surgery of the affected side. The median Tegner activity scale was 6 (range, 3 to 10). The mean KOOS subscores were 88.8% (Function/Sports), 86.6% (Quality of life), 94.6 (Symptoms), 94.0 (Pain) and 97.0 (Activities of Daily Living). Conclusion: Primary ACL repair, strictly limited to proximal ruptures with good tissue quality leads to revision rates and subjective outcome comparable to ACL reconstruction. Level of evidence: IV. KW - Regeneration Y1 - SP - 185 EP - 190 ER - TY - CHAP A1 - Hackethal, Johannes A1 - Schuh, Christina A1 - Hofer, Alexandra A1 - Meixner, Barbara A1 - Hennerbichler, Simone A1 - Redl, Heinz A1 - Teuschl, Andreas T1 - Human Placenta Laminin-111 as a Multifunctional Protein for Tissue Engineering and Regenerative Medicine T2 - Advances in Experimental Medicine and Biology KW - Biomaterial KW - Tissue Engineering KW - Regenerative medicine Y1 - PB - Springer ER - TY - GEN A1 - Drauschke, Andreas T1 - Neues aus der Welt der Optik - von Laserschwertern und Tarnvorrichtungen KW - Optics Y1 - ER - TY - GEN A1 - Drauschke, Andreas T1 - Effiziente Solarzellen mit Metamaterialien KW - Photonics Y1 - ER - TY - CHAP A1 - Tkachenko Bril, Andrés Igor A1 - Jagos, Harald A1 - David, Veronika A1 - Pils, Katharina A1 - Gaudernak, Jakob A1 - Rafolt, Dietmar ED - Schreier, Günter ED - Hayn, Dieter T1 - Proof of Concept of a Partial Weight-Bearing Supporting Real-Time Feedback System T2 - Health Informatics meets eHealth - Proceedings of the 12th eHealth Conference KW - Ambulation KW - Weight-Bearing KW - Patient KW - Feedback Y1 - PB - IOS Press CY - Amsterdam ER - TY - CHAP A1 - Franzl, Gerald A1 - Reif, Valerie A1 - Berger, Angela A1 - Frohner, Matthias A1 - Gottschalk, Marion A1 - Koch, Georg T1 - Interoperabilität im Datenaustausch in der Energiewirtschaft vom Use Case zum Test der Integrationsprofile T2 - 15. Symposium Energieinnovation KW - Interoperability KW - Virtual Power Plant KW - Smart Grid KW - Power Supply Y1 - ER - TY - GEN A1 - Franzl, Gerald A1 - Reif, Valerie A1 - Berger, Angela A1 - Frohner, Matthias A1 - Gottschalk, Marion A1 - Koch, Georg T1 - Interoperabilität im Datenaustausch in der Energiewirtschaft vom Use Case zum Test der Integrationsprofile KW - Interoperability KW - Virtual Power Plant KW - Smart Grid KW - Power Supply Y1 - ER - TY - GEN A1 - Tkachenko Bril, Andrés Igor A1 - Jagos, Harald A1 - David, Veronika A1 - Pils, Katharina A1 - Gaudernak, Jakob A1 - Rafolt, Dietmar T1 - Proof of Concept of a Partial Weight-Bearing Supporting Real-Time Feedback System KW - Ambulation KW - Weight-Bearing KW - Patient KW - Feedback Y1 - ER - TY - GEN A1 - Drauschke, Andreas T1 - Applications of Optogenetics KW - Optogenetics Y1 - 2018 ER - TY - GEN A1 - Veigl, Christoph A1 - David, Veronika T1 - AsTeRICS und FlipMouse: Flexible Eingabesysteme und kostengünstige Lösungen für Kommunikation und Umgebungssteuerung für Menschen mit Behinderung KW - Communication KW - Assistive Technology KW - AsTeRICS framework Y1 - ER - TY - RPRT A1 - Freistetter, Florian T1 - The Power of Lauf KW - Bioreactor KW - Muscle KW - Tissue Engineering Y1 - ER - TY - JOUR A1 - Berkovitch, Yulia A1 - Cohen, Talia A1 - Peled, Eli A1 - Schmidhammer, Robert A1 - Hildner, Florian A1 - Teuschl, Andreas A1 - Wolbank, Susanne A1 - Yelin, Dvir A1 - Redl, Heinz A1 - Seliktar, Dror T1 - Hydrogel Composition and Laser Micro-Patterning to Regulate Sciatic Nerve Regeneration JF - Journal of Tissue Engineering and Regenerative Medicine KW - Micro-Patterning KW - Laser KW - Nerve Regeneration Y1 - 2018 VL - 12 IS - 4 ER - TY - GEN A1 - Sabo, Anton A1 - Eckelt, Markus T1 - Running Shoes - Possible Correlations of Biomechanical and Material Tests KW - Shoes KW - Testing Y1 - ER - TY - GEN A1 - Eckelt, Markus T1 - Customizing Shoe Insoles through CAD-FEM Modelling KW - Customized Equipment KW - Arch ratio KW - Footwear design KW - Contact pressure KW - 3D Anthropometry Y1 - ER - TY - JOUR A1 - Bernhard, Jonathan A1 - Ferguson, James A1 - Rieder, Bernhard A1 - Heimel, Patrick A1 - Nau, Thomas A1 - Tangl, Stefan A1 - Redl, Heinz A1 - Vunjak-Novakovic, Gordana T1 - Tissue-engineered hypertrophic chondrocyte grafts enhanced long bone repair biomaterials JF - Biomaterials KW - Grafting KW - Biomaterials Y1 - 2018 IS - 139 SP - 202 EP - 212 ER - TY - GEN A1 - Eckelt, Markus T1 - Evaluating the Interaction Between Rim and Brake Pad on a Test Bench KW - Brake KW - Wheels KW - Test Bench Y1 - ER - TY - JOUR A1 - Heher, Philipp A1 - Maleiner, Babette A1 - Prüller, Johanna A1 - Teuschl, Andreas A1 - Kollmitzer, Josef A1 - Monforte Vila, Xavier A1 - Wolbank, Susanne A1 - Redl, Heinz A1 - Rünzler, Dominik A1 - Fuchs, Christiane T1 - A novel bioreactor for the generation of highly aligned 3D skeletal muscle-like constructs through orientation of fibrin via application of static strain JF - Acta Biomaterialia KW - Bioreactor Y1 - ER - TY - JOUR A1 - Jagos, Harald A1 - David, Veronika A1 - Reichel, Martin A1 - Kotzian, Stefan A1 - Schlossarek, Stefan A1 - Haller, Michael A1 - Rafolt, Dietmar T1 - Tele-monitoring of the rehabilitation progress in stroke patients JF - Studies in Health Technology and Informatics KW - Rehabilitation KW - Stroke Patients Y1 - 2018 ER - TY - CHAP A1 - Litzenberger, Stefan A1 - Christensen, Troels A1 - Hofstätter, Otto A1 - Sabo, Anton T1 - Prediction of Road Surface Quality during Cycling Using Smartphone Accelerometer Data T2 - 12th Conference of the International Sports Engineering Association KW - Cycling KW - Road Surface KW - Machine Learning KW - Accelerometer Data Y1 - ER - TY - GEN A1 - Sauermann, Stefan A1 - David, Veronika A1 - Schlögl, Alois A1 - Egelkraut, Reinhard A1 - Frohner, Matthias A1 - Pohn, Birgit A1 - Urbauer, Philipp A1 - Mense, Alexander T1 - Biosignals, Standards and FHIR – The Way to Go? KW - Biosignals KW - IT Standards Y1 - 2018 ER - TY - CHAP A1 - Frohner, Matthias A1 - Meyer, Markus A1 - Donsa, Klaus A1 - Urbauer, Philipp A1 - David, Veronika A1 - Sauermann, Stefan T1 - Telemonitoring of Blood Glucose - A Prototype Android Application Enhancing the Patient / Health Professional Experience Using Health IT Communication Standards T2 - DSAI 2018 - 8th International Conference on Software Development and Technologies for Enhancing Accessibility and Fighting Info-exclusion KW - Mobile Application KW - Blood Glucose KW - Telemonitoring KW - Communication Standards Y1 - ER - TY - GEN A1 - Frohner, Matthias A1 - Meyer, Markus A1 - Donsa, Klaus A1 - Urbauer, Philipp A1 - David, Veronika A1 - Sauermann, Stefan T1 - Telemonitoring of Blood Glucose - A Prototype Android Application Enhancing the Patient / Health Professional Experience Using Health IT Communication Standards KW - Mobile Application KW - Blood Glucose KW - Telemonitoring KW - Communication Standards Y1 - ER - TY - CHAP A1 - Sauermann, Stefan A1 - David, Veronika A1 - Schlögl, Alois A1 - Egelkraut, Reinhard A1 - Frohner, Matthias A1 - Pohn, Birgit A1 - Urbauer, Philipp A1 - Mense, Alexander T1 - Biosignals, Standards and FHIR – The Way to Go? T2 - Stud Health Technol Inform KW - Biosignals KW - Standards Y1 - 2018 SP - 356 EP - 362 ER - TY - GEN A1 - Sauermann, Stefan T1 - IHE / Compliance - Interoperabilität und Hygiene – gibt es Gemeinsamkeiten? KW - Telemonitoring KW - ELGA KW - Interoperability KW - Standardization Y1 - ER - TY - GEN A1 - Litzenberger, Stefan A1 - Christensen, Troels A1 - Hofstätter, Otto A1 - Sabo, Anton T1 - Prediction of Road Surface Quality during Cycling Using Smartphone Accelerometer Data KW - Cycling KW - Road Surface KW - Machine Learning KW - Accelerometer Data Y1 - ER - TY - GEN A1 - Sauermann, Stefan T1 - Flashlight: Telemonitoring Rahmenrichtlinie KW - Telemonitoring KW - ELGA KW - Standardization KW - Interoperability KW - Telehealth Y1 - ER - TY - CHAP A1 - Gastinger, Roman A1 - Litzenberger, Stefan A1 - Sabo, Anton T1 - Design, development and construction of a monitoring table tennis net T2 - Procedia Engineering KW - Sport KW - Tennis Y1 - 2019 SP - 297 EP - 303 ER - TY - GEN A1 - Sauermann, Stefan A1 - Frohner, Matthias T1 - Wie vernetzen wir Medizintechnik und IT in OP und ICU KW - Surgery KW - Intensive Care unit KW - Standardization KW - Telemonitoring KW - Digital Healthcare Y1 - ER - TY - GEN A1 - Sauermann, Stefan T1 - Digital Healthcare als Werkzeug für die nahtlose Integrierte Versorgung KW - Telemedicine KW - Telehealth KW - Decision Making KW - ELGA KW - Standardization Y1 - ER - TY - GEN A1 - Sauermann, Stefan T1 - Die digitale Revolution und ihre Auswirkungen auf die Gesellschaft KW - Interoperability KW - Standardization KW - eHealth KW - ELGA KW - Digital Healthcare Y1 - ER - TY - GEN A1 - Frohner, Matthias A1 - Forjan, Mathias T1 - Interoperability in the OR Introducing OR.NET KW - Interoperability KW - Emergency Room KW - Harmonization Y1 - ER - TY - RPRT A1 - Pasteka, Richard T1 - Analysis of influences on inhalable aerosols under characteristic respiratory simulation conditions KW - Mechanical Lung KW - Aerosol KW - Breathing KW - Respiratory system KW - Airflow Y1 - ER - TY - CHAP A1 - David, Veronika A1 - Forjan, Mathias A1 - Paštěka, Richard A1 - Scherer, Matthias A1 - Hofstätter, Otto T1 - Development of a Multi-Purpose Easy-to-Use Set of Tools for Home Based Rehabilitation T2 - DSAI 2018 - 8th International Conference on Software Development and Technologies for Enhancing Accessibility and Fighting Info-exclusion KW - Rehabitation KW - Low-Cost KW - eHealth KW - Home-based Rehabilitation KW - Telerehabilitation Y1 - ER - TY - CHAP A1 - Pasteka, Richard A1 - Forjan, Mathias A1 - David, Veronika T1 - A Single Point of Contact Data Platform for Rehabilitative Exercises and Equipment T2 - DSAI 2018 - 8th International Conference on Software Development and Technologies for Enhancing Accessibility and Fighting Info-exclusion KW - Telemedicine KW - Rehabilitation KW - Exercise database KW - Home based rehabilitation Y1 - ER - TY - GEN A1 - Scherer, Matthias A1 - Zapf, Stefan A1 - Salomon, Cornelia A1 - Kotzian, Stefan A1 - Forjan, Mathias T1 - Development of a virtual environment for the rehabilitation of neurological patients KW - Virtual Supermarket KW - Neurological Rehabilitation KW - Simulator Sickness KW - Rehabilitation Y1 - ER - TY - CHAP A1 - Frohner, Matthias A1 - Gottschalk, Marion A1 - Franzl, Gerald A1 - Pasteka, Richard A1 - Uslar, Mathias A1 - Sauermann, Stefan T1 - Smart Grid Interoperability Profiles Development T2 - IEEE International Conference on Smart Grid Communications KW - Smart Grid KW - Interoperability KW - Computer Architecture KW - Medical Services KW - Standards Y1 - ER - TY - GEN A1 - Tkachenko Bril, Andres Igor A1 - David, Veronika A1 - Forjan, Mathias A1 - Gaudernak, Jakob A1 - Pils, Katharina T1 - Evaluation of the feasibility of a partial weight-bearing supporting biofeedback system based on instrumented insoles and an Android application KW - Weight-Bearing KW - Insoles KW - Biofeedback System KW - Mobile Application KW - Pilot Study Y1 - ER - TY - GEN A1 - David, Veronika A1 - Forjan, Mathias A1 - Paštěka, Richard A1 - Scherer, Matthias A1 - Hofstätter, Otto T1 - Development of a Multi-Purpose Easy-to-Use Set of Tools for Home Based Rehabilitation KW - Rehabitation KW - Low-Cost KW - eHealth KW - Home-based Rehabilitation KW - Telerehabilitation Y1 - ER - TY - GEN A1 - Frohner, Matthias A1 - Forjan, Mathias T1 - Interoperability Challenges - How to integrate medical devices KW - Interoperability KW - eHealth KW - Hospital Y1 - ER - TY - GEN A1 - David, Veronika A1 - Forjan, Mathias T1 - Rehabilitation at Home - a Device supported Approach KW - Rehabilitation KW - Individualized Rehabilitation KW - Weight bearing KW - Exercise database KW - eHealth Y1 - ER - TY - GEN A1 - Mühleder, Severin A1 - Fuchs, Christiane A1 - Bassilio, Jose A1 - Sczwarc, Dorota A1 - Pill, Karoline A1 - Slezak, Paul A1 - Labuda, Krystina A1 - Siehs, Christian A1 - Pröll, Johannes A1 - Priglinger, Eleni A1 - Redl, Heinz A1 - Holnthoner, Wolfgang T1 - The purinergic receptor P2Y2 modulates endothelial sprouting and angiogenesis KW - Angiogenesis Y1 - 2018 ER - TY - JOUR A1 - Schneider, Karl Heinrich A1 - Enayati, Marjan A1 - Grasl, Christian A1 - Walter, Ingrid A1 - Budinsky, Lubos A1 - Zebic, Gabriel A1 - Kaun, Christoph A1 - Wagner, Anja A1 - Kratochwill, Klaus A1 - Redl, Heinz A1 - Teuschl, Andreas A1 - Podesser, Bruno K. A1 - Bergmeister, Helga T1 - Acellular vascular matrix grafts from human placenta chorion: Impact of ECM preservation on graft characteristics, protein composition and in vivo performance. JF - Biomaterials N2 - Small diameter vascular grafts from human placenta, decellularized with either Triton X-100 (Triton) or SDS and crosslinked with heparin were constructed and characterized. Graft biochemical properties, residual DNA, and protein composition were evaluated to compare the effect of the two detergents on graft matrix composition and structural alterations. Biocompatibility was tested in vitro by culturing the grafts with primary human macrophages and in vivo by subcutaneous implantation of graft conduits (n = 7 per group) into the flanks of nude rats. Subsequently, graft performance was evaluated using an aortic implantation model in Sprague Dawley rats (one month, n = 14). In situ graft imaging was performed using MRI angiography. Retrieved specimens were analyzed by electromyography, scanning electron microscopy, histology and immunohistochemistry to evaluate cell migration and the degree of functional tissue remodeling. Both decellularization methods resulted in grafts of excellent biocompatibility in vitro and in vivo, with low immunogenic potential. Proteomic data revealed removal of cytoplasmic proteins with relative enrichment of ECM proteins in decelluarized specimens of both groups. Noteworthy, LC-Mass Spectrometry analysis revealed that 16 proteins were exclusively preserved in Triton decellularized specimens in comparison to SDS-treated specimens. Aortic grafts showed high patency rates, no signs of thrombus formation, aneurysms or rupture. Conduits of both groups revealed tissue-specific cell migration indicative of functional remodeling. This study strongly suggests that decellularized allogenic grafts from the human placenta have the potential to be used as vascular replacement materials. Both detergents produced grafts with low residual immunogenicity and appropriate mechanical properties. Observed differences in graft characteristics due to preservation method had no impact on successful in vivo performance in the rodent model. KW - Biomaterial KW - Tissue Engineering Y1 - SP - 14 EP - 26 ER - TY - JOUR A1 - Priglinger, Eleni A1 - Schuh, Christina A1 - Steffenhagen, Carolin A1 - Wurzer, Christoph A1 - Maier, Julia A1 - Nürnberger, Sylvia A1 - Holnthoner, Wolfgang A1 - Fuchs, Christiane A1 - Suessner, Susanne A1 - Rünzler, Dominik A1 - Redl, Heinz A1 - Wolbank, Susanne T1 - Improvement of adipose tissue-derived cells by low-energy extracorporeal shock wave therapy. JF - Cytotherapy N2 - BACKGROUND: Cell-based therapies with autologous adipose tissue-derived cells have shown great potential in several clinical studies in the last decades. The majority of these studies have been using the stromal vascular fraction (SVF), a heterogeneous mixture of fibroblasts, lymphocytes, monocytes/macrophages, endothelial cells, endothelial progenitor cells, pericytes and adipose-derived stromal/stem cells (ASC) among others. Although possible clinical applications of autologous adipose tissue-derived cells are manifold, they are limited by insufficient uniformity in cell identity and regenerative potency. METHODS: In our experimental set-up, low-energy extracorporeal shock wave therapy (ESWT) was performed on freshly obtained human adipose tissue and isolated adipose tissue SVF cells aiming to equalize and enhance stem cell properties and functionality. RESULTS: After ESWT on adipose tissue we could achieve higher cellular adenosine triphosphate (ATP) levels compared with ESWT on the isolated SVF as well as the control. ESWT on adipose tissue resulted in a significantly higher expression of single mesenchymal and vascular marker compared with untreated control. Analysis of SVF protein secretome revealed a significant enhancement in insulin-like growth factor (IGF)-1 and placental growth factor (PLGF) after ESWT on adipose tissue. DISCUSSION: Summarizing we could show that ESWT on adipose tissue enhanced the cellular ATP content and modified the expression of single mesenchymal and vascular marker, and thus potentially provides a more regenerative cell population. Because the effectiveness of autologous cell therapy is dependent on the therapeutic potency of the patient's cells, this technology might raise the number of patients eligible for autologous cell transplantation. KW - Shockwave Therapy KW - Tissue Regeneration KW - Regenerative Medicine Y1 - SP - 1079 EP - 1095 ER - TY - JOUR A1 - Bachmann, Barbara A1 - Spitz, Sarah A1 - Rothbauer, Mario A1 - Jordan, Christian A1 - Purtscher, Michaela A1 - Zirath, Helene A1 - Schuller, Patrick A1 - Eilenberger, Christoph A1 - Ali, Syed Faheem A1 - Mühleder, Severin A1 - Priglinger, Eleni A1 - Harasek, Michael A1 - Redl, Heinz A1 - Holnthoner, Wolfgang A1 - Ertl, Peter T1 - Engineering of three-dimensional pre-vascular networks within fibrin hydrogel constructs by microfluidic control over reciprocal cell signaling JF - Biomicrofluidics KW - Microfluidic KW - Vascularization KW - Tissue Engineering Y1 - 2019 ER - TY - GEN A1 - Traxler, Lukas T1 - Klare Sicht nach Grauem Star KW - Intraocular Lenses KW - Cataract KW - Testing method Y1 - ER -