TY  - JOUR
A1  - Priglinger, Eleni
A1  - Schuh, Christina
A1  - Steffenhagen, Carolin
A1  - Wurzer, Christoph
A1  - Maier, Julia
A1  - Nürnberger, Sylvia
A1  - Holnthoner, Wolfgang
A1  - Fuchs, Christiane
A1  - Suessner, Susanne
A1  - Rünzler, Dominik
A1  - Redl, Heinz
A1  - Wolbank, Susanne
T1  - Improvement of adipose tissue-derived cells by low-energy extracorporeal shock wave therapy.
JF  - Cytotherapy
N2  - BACKGROUND:

Cell-based therapies with autologous adipose tissue-derived cells have shown great potential in several clinical studies in the last decades. The majority of these studies have been using the stromal vascular fraction (SVF), a heterogeneous mixture of fibroblasts, lymphocytes, monocytes/macrophages, endothelial cells, endothelial progenitor cells, pericytes and adipose-derived stromal/stem cells (ASC) among others. Although possible clinical applications of autologous adipose tissue-derived cells are manifold, they are limited by insufficient uniformity in cell identity and regenerative potency.
METHODS:

In our experimental set-up, low-energy extracorporeal shock wave therapy (ESWT) was performed on freshly obtained human adipose tissue and isolated adipose tissue SVF cells aiming to equalize and enhance stem cell properties and functionality.
RESULTS:

After ESWT on adipose tissue we could achieve higher cellular adenosine triphosphate (ATP) levels compared with ESWT on the isolated SVF as well as the control. ESWT on adipose tissue resulted in a significantly higher expression of single mesenchymal and vascular marker compared with untreated control. Analysis of SVF protein secretome revealed a significant enhancement in insulin-like growth factor (IGF)-1 and placental growth factor (PLGF) after ESWT on adipose tissue.
DISCUSSION:

Summarizing we could show that ESWT on adipose tissue enhanced the cellular ATP content and modified the expression of single mesenchymal and vascular marker, and thus potentially provides a more regenerative cell population. Because the effectiveness of autologous cell therapy is dependent on the therapeutic potency of the patient's cells, this technology might raise the number of patients eligible for autologous cell transplantation.
KW  - Shockwave Therapy
KW  - Tissue Regeneration
KW  - Regenerative Medicine
Y1  - 
SP  - 1079
EP  - 1095
ER  -