TY - JOUR A1 - Mandl, Thomas A1 - Meyerspeer, Martin A1 - Reichel, Martin A1 - Kern, Helmut A1 - Hofer, Christian A1 - Mayr, Winfried A1 - Moser, Ewald T1 - Functional electrical stimulation of long-term denervated, degenerated human skeletal muscle: estimating activation using T2-parameter magnetic resonance imaging methods JF - Artif Organs KW - Electrical Stimulation KW - Muscle Stimulation Y1 - 2019 IS - 32(8) SP - 604 EP - 608 ER - TY - GEN A1 - Teuschl, Andreas A1 - Fuchs, Christiane T1 - Bioreactors in Musculoskeletal Tissue Engineering KW - Bioreactor KW - Tissue Engineering Y1 - ER - TY - JOUR A1 - Maleiner, Babette A1 - Tomasch, Janine A1 - Heher, Philipp A1 - Spadiut, Oliver A1 - Rünzler, Dominik A1 - Fuchs, Christiane T1 - The Importance of Biophysical and Biochemical Stimuli in Dynamic Skeletal Muscle Models. JF - Frontiers in Physiology N2 - Classical approaches to engineer skeletal muscle tissue based on current regenerative and surgical procedures still do not meet the desired outcome for patient applications. Besides the evident need to create functional skeletal muscle tissue for the repair of volumetric muscle defects, there is also growing demand for platforms to study muscle-related diseases, such as muscular dystrophies or sarcopenia. Currently, numerous studies exist that have employed a variety of biomaterials, cell types and strategies for maturation of skeletal muscle tissue in 2D and 3D environments. However, researchers are just at the beginning of understanding the impact of different culture settings and their biochemical (growth factors and chemical changes) and biophysical cues (mechanical properties) on myogenesis. With this review we intend to emphasize the need for new in vitro skeletal muscle (disease) models to better recapitulate important structural and functional aspects of muscle development. We highlight the importance of choosing appropriate system components, e.g., cell and biomaterial type, structural and mechanical matrix properties or culture format, and how understanding their interplay will enable researchers to create optimized platforms to investigate myogenesis in healthy and diseased tissue. Thus, we aim to deliver guidelines for experimental designs to allow estimation of the potential influence of the selected skeletal muscle tissue engineering setup on the myogenic outcome prior to their implementation. Moreover, we offer a workflow to facilitate identifying and selecting different analytical tools to demonstrate the successful creation of functional skeletal muscle tissue. Ultimately, a refinement of existing strategies will lead to further progression in understanding important aspects of muscle diseases, muscle aging and muscle regeneration to improve quality of life of patients and enable the establishment of new treatment options. KW - Bioreactor KW - Muscle KW - Biomaterial Y1 - ER - TY - JOUR A1 - Tomasch, Janine A1 - Maleiner, Babette A1 - Heher, Philipp A1 - Rufin, Manuel A1 - Andriotis, Orestis G. A1 - Thurner, Philipp J. A1 - Redl, Heinz A1 - Fuchs, Christiane A1 - Teuschl-Woller, Andreas H. T1 - Changes in Elastic Moduli of Fibrin Hydrogels Within the Myogenic Range Alter Behavior of Murine C2C12 and Human C25 Myoblasts Differently JF - Froniers in Bioengineering and Biotechnology N2 - Fibrin hydrogels have proven highly suitable scaffold materials for skeletal muscle tissue engineering in the past. Certain parameters of those types of scaffolds, however, greatly affect cellular mechanobiology and therefore the myogenic outcome. The aim of this study was to identify the influence of apparent elastic properties of fibrin scaffolds in 2D and 3D on myoblasts and evaluate if those effects differ between murine and human cells. Therefore, myoblasts were cultured on fibrin-coated multiwell plates (“2D”) or embedded in fibrin hydrogels (“3D”) with different elastic moduli. Firstly, we established an almost linear correlation between hydrogels’ fibrinogen concentrations and apparent elastic moduli in the range of 7.5 mg/ml to 30 mg/ml fibrinogen (corresponds to a range of 7.7–30.9 kPa). The effects of fibrin hydrogel elastic modulus on myoblast proliferation changed depending on culture type (2D vs 3D) with an inhibitory effect at higher fibrinogen concentrations in 3D gels and vice versa in 2D. The opposite effect was evident in differentiating myoblasts as shown by gene expression analysis of myogenesis marker genes and altered myotube morphology. Furthermore, culture in a 3D environment slowed down proliferation compared to 2D, with a significantly more pronounced effect on human myoblasts. Differentiation potential was also substantially impaired upon incorporation into 3D gels in human, but not in murine, myoblasts. With this study, we gained further insight in the influence of apparent elastic modulus and culture type on cellular behavior and myogenic outcome of skeletal muscle tissue engineering approaches. Furthermore, the results highlight the need to adapt parameters of 3D culture setups established for murine cells when applied to human cells. KW - Tissue Engineering KW - Fibrin KW - Hydrogel KW - Biomaterials KW - Cell Culture Y1 - VL - 10 SP - 836520 ER - TY - JOUR A1 - Angelova, Liliya A1 - Daskalova, Albena A1 - Filipov, Emil A1 - Monforte Vila, Xavier A1 - Tomasch, Janine A1 - Avdeev, Georgi A1 - Teuschl-Woller, Andreas Herbert A1 - Buchvarov, Ivan T1 - Optimizing the Surface Structural and Morphological Properties of Silk Thin Films via Ultra-Short Laser Texturing for Creation of Muscle Cell Matrix Model JF - Polymers N2 - Temporary scaffolds that mimic the extracellular matrix's structure and provide a stable substratum for the natural growth of cells are an innovative trend in the field of tissue engineering. The aim of this study is to obtain and design porous 2D fibroin-based cell matrices by femtosecond laser-induced microstructuring for future applications in muscle tissue engineering. Ultra-fast laser treatment is a non-contact method, which generates controlled porosity-the creation of micro/nanostructures on the surface of the biopolymer that can strongly affect cell behavior, while the control over its surface characteristics has the potential of directing the growth of future muscle tissue in the desired direction. The laser structured 2D thin film matrices from silk were characterized by means of SEM, EDX, AFM, FTIR, Micro-Raman, XRD, and 3D-roughness analyses. A WCA evaluation and initial experiments with murine C2C12 myoblasts cells were also performed. The results show that by varying the laser parameters, a different structuring degree can be achieved through the initial lifting and ejection of the material around the area of laser interaction to generate porous channels with varying widths and depths. The proper optimization of the applied laser parameters can significantly improve the bioactive properties of the investigated 2D model of a muscle cell matrix. Keywords: biopolymers; femtosecond laser processing; muscle cell matrix 2D model; muscle tissue engineering; silk fibroin. KW - Tissue Engineering KW - Muscle Cell matrix Model KW - Silk Scaffold KW - Surface Structure Y1 - VL - 2022 IS - 14(13), 2584 ER - TY - GEN A1 - Heher, Philipp A1 - Tomasch, Janine A1 - Maleiner, Babette A1 - Redl, Heinz A1 - Fuchs, Christiane T1 - The Importance of Biomechanical Cues for In Vitro Skeletal Myogenesis KW - In Vitro KW - Myogenesis Y1 - 2018 ER - TY - GEN A1 - Tomasch, Janine T1 - Generation of 3D skeletal muscle-like scaffolds via the application of mechanical stimuli KW - Scaffold KW - Mechanical Stimuli Y1 - 2018 ER - TY - CHAP A1 - Mally, Franziska A1 - Hofstätter, Otto A1 - Eckelt, Markus T1 - Influence of Running Shoes and Running Velocity on “Ride” during Running. T2 - ISEA 2020 Online - Proceedings 2020 KW - Sports Equipment Technologies KW - Running Shoes KW - Footwear Y1 - 2020 ER - TY - JOUR A1 - Schuh, Christina A1 - Heher, Philipp A1 - Weihs, Anna A1 - Fuchs, Christiane A1 - Gabriel, Christian A1 - Wolbank, Susanne A1 - Mittermayr, Rainer A1 - Redl, Heinz A1 - Rünzler, Dominik A1 - Teuschl, Andreas T1 - In vitro extracorporeal shock wave treatment enhances stemness and preserves multipotency of rat and human adipose-derived stem cells JF - Journal of Cytotherapy KW - Shockwave Y1 - ER - TY - CHAP A1 - Meyer, Markus A1 - Donsa, Klaus A1 - Truskaller, Thomas A1 - Frohner, Matthias A1 - Pohn, Birgit A1 - Felfernig, Alexander A1 - Sinner, Franz A1 - Pieber, Thomas T1 - Development of a Protocol for Automated Glucose Measurement Transmission Used in Clinical Decision Support Systems Based on the Continua Design Guidelines T2 - Conference Proceedings eHealth 2018 KW - Mobile Health KW - Standardization KW - Clinical Decision Support Systems KW - Diabetes KW - Medication Errors Y1 - ER - TY - GEN A1 - Meyer, Markus A1 - Donsa, Klaus A1 - Truskaller, Thomas A1 - Frohner, Matthias A1 - Pohn, Birgit A1 - Felfernig, Alexander A1 - Sinner, Franz A1 - Pieber, Thomas T1 - Development of a Protocol for Automated Glucose Measurement Transmission Used in Clinical Decision Support Systems Based on the Continua Design Guidelines KW - Mobile Health KW - Standardization KW - Clinical Decision Support Systems KW - Diabetes KW - Medication Errors Y1 - ER - TY - GEN A1 - Mandl, Thomas A1 - Dohnal, Fahdi A1 - Stickler, Yvonne A1 - Martinek, Johannes A1 - Reichel, Martin A1 - Mayr, Winfried A1 - Rattay, Frank T1 - FE-Modellierung für Implantate als Alternative zur transkutanen Elektrostimulation des langzeitdenervierten Oberschenkels KW - Implants KW - Denervation Y1 - 2019 ER - TY - GEN A1 - Kornfeind, P. A1 - Blaha, A. A1 - Peuschl, M. A1 - Reichel, Martin A1 - Sabo, Anton T1 - 2-Komponentenplattform zur Erfassung der Stemmbrettkräfte am Ruderergometer und im Ruderboot KW - Sport KW - Rowing Y1 - 2019 ER - TY - JOUR A1 - Purtscher, Michaela A1 - Rothbauer, Mario A1 - Kratz, Sebastian Rudi Adam A1 - Bailey, Andrew A1 - Lieberzeit, Peter A1 - Ertl, Peter T1 - A microfluidic impedance-based extended infectivity assay: combining retroviral amplification and cytopathic effect monitoring on a single lab-on-a-chip platform JF - Lab on a Chip N2 - Detection, quantification and monitoring of virus – host cell interactions are of great importance when evaluating the safety of pharmaceutical products. With the wide usage of viral based vector systems in combination with mammalian cell lines for the production of biopharmaceuticals, the presence of replication competent viral particles needs to be avoided and potential hazards carefully assessed. Consequently, regulatory agencies recommend viral clearance studies using plaque assays or TCID50 assays to evaluate the efficiency of the production process in removing viruses. While plaque assays provide reliable information on the presence of viral contaminations, they are still tedious to perform and can take up to two weeks to finish. To overcome some of these limitations, we have automated, miniaturized and integrated the dual cell culture bioassay into a common lab-on-a-chip platform containing embedded electrical sensor arrays to enrich and detect infectious viruses. Results of our microfluidic single step assay show that a significant reduction in assay time down to 3 to 4 days can be achieved using simultaneous cell-based viral amplification, release and detection of cytopathic effects in a target cell line. We further demonstrate the enhancing effect of continuous fluid flow on infection of PG-4 reporter cells by newly formed and highly active virions by M. dunni cells, thus pointing to the importance of physical relevant viral–cell interactions. KW - Tissue Engineering KW - Microfluidics KW - Lab on Chip Y1 - 2021 VL - 2021 IS - Issue 7 SP - 1364 EP - 1372 ER - TY - JOUR A1 - Zupkovitz, Gordin A1 - Kabiljo, Julijan A1 - Kothmayer, Michael A1 - Schlick, Katharina A1 - Schöfer, Christian A1 - Lagger, Sabine A1 - Pusch, Oliver T1 - Analysis of Methylation Dynamics Reveals a Tissue-Specific, Age-Dependent Decline in 5-Methylcytosine Within the Genome of the Vertebrate Aging Model Nothobranchius furzeri. JF - Frontiers in Molecular Biosciences N2 - Erosion of the epigenetic DNA methylation landscape is a widely recognized hallmark of aging. Emerging advances in high throughput sequencing techniques, in particular DNA methylation data analysis, have resulted in the establishment of precise human and murine age prediction tools. In vertebrates, methylation of cytosine at the C5 position of CpG dinucleotides is executed by DNA methyltransferases (DNMTs) whereas the process of enzymatic demethylation is highly dependent on the activity of the ten-eleven translocation methylcytosine dioxygenase (TET) family of enzymes. Here, we report the identification of the key players constituting the DNA methylation machinery in the short-lived teleost aging model Nothobranchius furzeri. We present a comprehensive spatio-temporal expression profile of the methylation-associated enzymes from embryogenesis into late adulthood, thereby covering the complete killifish life cycle. Data mining of the N. furzeri genome produced five dnmt gene family orthologues corresponding to the mammalian DNMTs (DNMT1, 2, 3A, and 3B). Comparable to other teleost species, N. furzeri harbors multiple genomic copies of the de novo DNA methylation subfamily. A related search for the DNMT1 recruitment factor UHRF1 and TET family members resulted in the identification of N. furzeri uhrf1, tet1, tet2, and tet3. Phylogenetic analysis revealed high cross-species similarity on the amino acid level of all individual dnmts, tets, and uhrf1, emphasizing a high degree of functional conservation. During early killifish development all analyzed dnmts and tets showed a similar expression profile characterized by a strong increase in transcript levels after fertilization, peaking either at embryonic day 6 or at the black eye stage of embryonic development. In adult N. furzeri, DNA methylation regulating enzymes showed a ubiquitous tissue distribution. Specifically, we observed an age-dependent downregulation of dnmts, and to some extent uhrf1, which correlated with a significant decrease in global DNA methylation levels in the aging killifish liver and muscle. The age-dependent DNA methylation profile and spatio-temporal expression characteristics of its enzymatic machinery reported here may serve as an essential platform for the identification of an epigenetic aging clock in the new vertebrate model system N. furzeri. KW - Tissue Engineering KW - Killifish KW - Aging Model Y1 - VL - 8 IS - 627143 ER - TY - CHAP A1 - Wießner, Katharina A1 - Machacek-Link, Thomas A1 - Leitner, Rita T1 - Encouraging the development of higher-order cognitive skills via applied exercises and web-based self-assessment to teach the basic principles in molecular biology. T2 - PIXEL NPSE2021, März 2021 N2 - The responsibility of a lecturer is not only to share his or her knowledge with the students in an easy to understand manner, but also to help the students to embed new knowledge and to encourage the development of higher-order cognitive skills via applied exercises. In order to meet the growing demand for blended learning approaches a new course concept was established in autumn 2018. To enhance comprehension and to provide opportunities for self-assessment, web-based training units were implemented by using the interactive learning software “Articulate Storyline”. Students had to prepare at home for the course units by completing interactive chapters. Their learning outcome was assessed by online quizzes at the end of each chapter. Online Training chapters allowed time to focus on selected topics and to repeat key messages in following presence units. Additionally, guided group exercises were performed to promote analytic skills and abstract thinking. The students had to apply and combine their knowledge to solve problem-based challenges. An optional revision course was offered to the students, which allowed for interactive repetition of the acquired knowledge with the focus on student-to-lecturer dialog. An analysis based on a written evaluation of this course resulted in a positive feedback from the students, in particular regarding the guided exercises and the offered revision course. According to the students the group exercises allowed to process the learned subjects, promoted the group climate and were a convenient diversion from the frontal lecture format. Students who attended the revision course on a regular basis showed a better performance at the final exam and exceeded especially at interdisciplinary questions. The first implementation of this master´s degree course indicated that the combination of web-based training elements with frontal lecture elements, guided exercises stimulating cognitive skills and an optional revision course can teach students the basics of biology in an understandable way. This course structure is especially applicable to teach basic subjects for groups of students with varying initial knowledge. Financial support from the City of Vienna project PBL in Molecular Life Science (21-06) is gratefully acknowledged. KW - PBL KW - Didaktik KW - Lehre Y1 - ER - TY - JOUR A1 - Deininger, Christian A1 - Wagner, Andrea A1 - Heimel, Patrick A1 - Salzer, Elias A1 - Monforte Vila, Xavier A1 - Weißenbacher, Nadja A1 - Grillari, Johannes A1 - Redl, Heinz A1 - Wichlas, Florian A1 - Freude, Thomas A1 - Tempfer, Herbert A1 - Teuschl-Woller, Andreas A1 - Traweger, Andreas T1 - Enhanced BMP-2-Mediated Bone Repair Using an Anisotropic Silk Fibroin Scaffold Coated with Bone-like Apatite JF - Int. J. Mol. Sci. N2 - The repair of large bone defects remains challenging and often requires graft material due to limited availability of autologous bone. In clinical settings, collagen sponges loaded with excessive amounts of bone morphogenetic protein 2 (rhBMP-2) are occasionally used for the treatment of bone non-unions, increasing the risk of adverse events. Therefore, strategies to reduce rhBMP-2 dosage are desirable. Silk scaffolds show great promise due to their favorable biocompatibility and their utility for various biofabrication methods. For this study, we generated silk scaffolds with axially aligned pores, which were subsequently treated with 10× simulated body fluid (SBF) to generate an apatitic calcium phosphate coating. Using a rat femoral critical sized defect model (CSD) we evaluated if the resulting scaffold allows the reduction of BMP-2 dosage to promote efficient bone repair by providing appropriate guidance cues. Highly porous, anisotropic silk scaffolds were produced, demonstrating good cytocompatibility in vitro and treatment with 10× SBF resulted in efficient surface coating. In vivo, the coated silk scaffolds loaded with a low dose of rhBMP-2 demonstrated significantly improved bone regeneration when compared to the unmineralized scaffold. Overall, our findings show that this simple and cost-efficient technique yields scaffolds that enhance rhBMP-2 mediated bone healing. KW - Tissue Engineering KW - Biomaterials KW - silk scaffold KW - bone regeneration KW - pseudoarthrosis Y1 - VL - 23 IS - 1 / 283 ER - TY - JOUR A1 - Hackethal, Johannes A1 - Dungel, Peter A1 - Teuschl, Andreas Herbert T1 - Frequently Used Strategies to Isolate Extracellular Matrix Proteins from Human Placenta and Adipose Tissue JF - Tissue Engineering Part C: Methods N2 - The natural extracellular matrix (ECM) provides the optimal environment for cells. Many enzymatic or non-enzymatic based strategies to extract ECM proteins from tissues were published over the past years. However, every single isolation strategy reported so far is associated with specific bottlenecks. In this study, frequently used strategies to isolate ECM from human placenta or adipose tissue using Tris-, serum-, or pepsin-based buffers were compared. The resulting ECM proteins were biochemically characterized by analysis of cellular remnants using Hoechst DNA staining, glycosaminoglycan (GAG) content by dimethylmethylene blue, visualization of protein bands using sodium dodecyl sulfate-polyacrylamide gel electrophoresis analysis combined with amino acid quantification, and assessment of the proangiogenic profile using an angiogenesis array. Tris-NaCl-extracted ECM proteins showed a high heterogenic degree of extracted proteins, bioactive growth factors, and GAGs, but no collagen-I. Active serum-extracted ECM showed significant lower DNA remnants when compared with the Tris-NaCl isolation strategy. Pepsin-extracted ECM was rich in collagen-I and low amounts of remaining bioactive growth factors. This strategy was most effective to reduce DNA amounts when compared with the other isolation strategies. Pepsin-extracted ECM from both tissues easily gelled at 37°C, whereas the other extracted ECM strategies did not gel at 37°C (Tris-NaCl: liquid; serum: sponge). All relevant characteristics (DNA residues, ECM diversity and bioactivity, shape) of the extracted ECM proteins highly depend on its isolation strategy and could still be optimized. Impact statement The natural human extracellular matrix (ECM) is the ideal cell niche. Various strategies were reported to isolate human ECM components from various sources. In this article, we compared frequently used methods and compared their characteristics (DNA remnants, glycosaminoglycan content, sodium dodecyl sulfate-polyacrylamide gel electrophoresis analysis, amino acid quantification, angiogenesis array, and gel formation). We conclude that more research is still necessary to optimize current isolation approaches for in vitro or in vivo applications of human ECM. KW - Tissue Engineering KW - Biomaterials KW - Adipose Tissue KW - extracellular matrix KW - human placenta Y1 - VL - 27 IS - 12 SP - 649 EP - 660 ER - TY - JOUR A1 - Khimich, Margarita A. A1 - Prosolov, Konstantin A. A1 - Mishurova, Tatiana A1 - Evsevleev, Sergej A1 - Monforte, Xavier A1 - Teuschl, Andreas H. A1 - Slezak, Paul A1 - Ibragimov, Egor A. A1 - Saprykin, Alexander A. A1 - Kovalevskaya, Zhanna G. A1 - Dmitriev, Andrey I. A1 - Bruno, Giovanni A1 - Sharkeev, Yurii P. T1 - Advances in Laser Additive Manufacturing of Ti-Nb Alloys: From Nanostructured Powders to Bulk Objects JF - Nanomaterials (Basel) N2 - The additive manufacturing of low elastic modulus alloys that have a certain level of porosity for biomedical needs is a growing area of research. Here, we show the results of manufacturing of porous and dense samples by a laser powder bed fusion (LPBF) of Ti-Nb alloy, using two distinctive fusion strategies. The nanostructured Ti-Nb alloy powders were produced by mechanical alloying and have a nanostructured state with nanosized grains up to 90 nm. The manufactured porous samples have pronounced open porosity and advanced roughness, contrary to dense samples with a relatively smooth surface profile. The structure of both types of samples after LPBF is formed by uniaxial grains having micro- and nanosized features. The inner structure of the porous samples is comprised of an open interconnected system of pores. The volume fraction of isolated porosity is 2 vol. % and the total porosity is 20 vol. %. Cell viability was assessed in vitro for 3 and 7 days using the MG63 cell line. With longer culture periods, cells showed an increased cell density over the entire surface of a porous Ti-Nb sample. Both types of samples are not cytotoxic and could be used for further in vivo studies. KW - Tissue Engineering KW - Biomaterials KW - Laser Additive Manufacturing KW - Bulk Objects Y1 - VL - 11 IS - 5 / 1159 ER - TY - JOUR A1 - Farokhi, Maryam A1 - Aleemardani, Mina A1 - Solouk, Atefeh A1 - Mirzadeh, Hamid A1 - Teuschl, Andreas Herbert A1 - Redl, Heinz T1 - Crosslinking strategies for silk fibroin hydrogels: promising biomedical materials JF - Biomedical Materials N2 - Due to their strong biomimetic potential, silk fibroin (SF) hydrogels are impressive candidates for tissue engineering, due to their tunable mechanical properties, biocompatibility, low immunotoxicity, controllable biodegradability, and a remarkable capacity for biomaterial modification and the realization of a specific molecular structure. The fundamental chemical and physical structure of SF allows its structure to be altered using various crosslinking strategies. The established crosslinking methods enable the formation of three-dimensional (3D) networks under physiological conditions. There are different chemical and physical crosslinking mechanisms available for the generation of SF hydrogels (SFHs). These methods, either chemical or physical, change the structure of SF and improve its mechanical stability, although each method has its advantages and disadvantages. While chemical crosslinking agents guarantee the mechanical strength of SFH through the generation of covalent bonds, they could cause some toxicity, and their usage is not compatible with a cell-friendly technology. On the other hand, physical crosslinking approaches have been implemented in the absence of chemical solvents by the induction of β-sheet conformation in the SF structure. Unfortunately, it is not easy to control the shape and properties of SFHs when using this method. The current review discusses the different crosslinking mechanisms of SFH in detail, in order to support the development of engineered SFHs for biomedical applications. KW - Tissue Engineering KW - hydrogels KW - Biomaterials KW - silk fibroin Y1 - VL - 16 IS - 2 SP - 022004 ER - TY - JOUR A1 - Schanda, Jakob A1 - Keibl, Claudia A1 - Heimel, Patrick A1 - Monforte, Xavier A1 - Feichtinger, Xaver A1 - Teuschl, Andreas A1 - Baierl, Andreas A1 - Muschitz, Christian A1 - Redl, Heinz A1 - Fialka, Christian A1 - Mittermayr, Rainer T1 - Zoledronic Acid Substantially Improves Bone Microarchitecture and Biomechanical Properties After Rotator Cuff Repair in a Rodent Chronic Defect Model JF - Am J Sports Med N2 - Background: Bone mineral density at the humeral head is reduced in patients with chronic rotator cuff tears. Bone loss in the humeral head is associated with repair failure after rotator cuff reconstruction. Bisphosphonates (eg, zoledronic acid) increase bone mineral density. Hypothesis: Zoledronic acid improves bone mineral density of the humeral head and biomechanical properties of the enthesis after reconstruction of chronic rotator cuff tears in rats. Study design: Controlled laboratory study. Methods: A total of 32 male Sprague-Dawley rats underwent unilateral (left) supraspinatus tenotomy with delayed transosseous rotator cuff reconstruction after 3 weeks. All rats were sacrificed 8 weeks after rotator cuff repair. Animals were randomly assigned to 1 of 2 groups. At 1 day after rotator cuff reconstruction, the intervention group was treated with a single subcutaneous dose of zoledronic acid at 100 µg/kg bodyweight, and the control group received 1 mL of subcutaneous saline solution. In 12 animals of each group, micro-computed tomography scans of both shoulders were performed as well as biomechanical testing of the supraspinatus enthesis of both sides. In 4 animals of each group, histological analyses were conducted. Results: In the intervention group, bone volume fraction (bone volume/total volume [BV/TV]) of the operated side was higher at the lateral humeral head (P = .005) and the medial humeral head (P = .010) compared with the control group. Trabecular number on the operated side was higher at the lateral humeral head (P = .004) and the medial humeral head (P = .001) in the intervention group. Maximum load to failure rates on the operated side were higher in the intervention group (P < .001). Cortical thickness positively correlated with higher maximum load to failure rates in the intervention group (r = 0.69; P = .026). Histological assessment revealed increased bone formation in the intervention group. Conclusion: Single-dose therapy of zoledronic acid provided an improvement of bone microarchitecture at the humeral head as well as an increase of maximum load to failure rates after transosseous reconstruction of chronic rotator cuff lesions in rats. Clinical relevance: Zoledronic acid improves bone microarchitecture as well as biomechanical properties after reconstruction of chronic rotator cuff tears in rodents. These results need to be verified in clinical investigations. KW - Tissue Engineering KW - Rotator Cuff Tears Y1 - VL - 2020 Jul IS - 48 (9) SP - 2151 EP - 2160 ER - TY - JOUR A1 - Bachmann, Barbara A1 - Spitz, Sarah A1 - Schädl, Barbara A1 - Teuschl, Andreas A1 - Redl, Heinz A1 - Nürnberger, Sylvia A1 - Ertl, Peter T1 - Stiffness Matters: Fine-Tuned Hydrogel Elasticity Alters Chondrogenic Redifferentiation JF - Froniers in Bioengineering and Biotechnology N2 - Biomechanical cues such as shear stress, stretching, compression, and matrix elasticity are vital in the establishment of next generation physiological in vitro tissue models. Matrix elasticity, for instance, is known to guide stem cell differentiation, influence healing processes and modulate extracellular matrix (ECM) deposition needed for tissue development and maintenance. To better understand the biomechanical effect of matrix elasticity on the formation of articular cartilage analogs in vitro, this study aims at assessing the redifferentiation capacity of primary human chondrocytes in three different hydrogel matrices of predefined matrix elasticities. The hydrogel elasticities were chosen to represent a broad spectrum of tissue stiffness ranging from very soft tissues with a Young's modulus of 1 kPa up to elasticities of 30 kPa, representative of the perichondral-space. In addition, the interplay of matrix elasticity and transforming growth factor beta-3 (TGF-β3) on the redifferentiation of primary human articular chondrocytes was studied by analyzing both qualitative (viability, morphology, histology) and quantitative (RT-qPCR, sGAG, DNA) parameters, crucial to the chondrotypic phenotype. Results show that fibrin hydrogels of 30 kPa Young's modulus best guide chondrocyte redifferentiation resulting in a native-like morphology as well as induces the synthesis of physiologic ECM constituents such as glycosaminoglycans (sGAG) and collagen type II. This comprehensive study sheds light onto the mechanobiological impact of matrix elasticity on formation and maintenance of articular cartilage and thus represents a major step toward meeting the need for advanced in vitro tissue models to study both re- and degeneration of articular cartilage. KW - Tissue Engineering KW - Chondrogenic Redifferentiation KW - Biomaterials Y1 - 2021 VL - 2020 IS - 8 SP - 373 ER - TY - JOUR A1 - Simböck, Elisabeth A1 - Marksteiner, Jessica A1 - Machacek, Thomas A1 - Wiessner, Katharina A1 - Gepp, Barbara A1 - Jesenberger, Veronika A1 - Weihs, Anna A1 - Leitner, Rita T1 - The Power of Problem Based Learning beyond its Didactic Attributes JF - Journal of Problem Based Learning in Higher Education (JPBLHE) N2 - Hybrid courses with a focus on practice-orientated education and self-guided learning phases are on the rise on the higher education sector. Disciplines in Life Sciences implicate a high degree of practical laboratory expertise. The University of Applied Sciences (UAS) in Vienna, Austria, has thus been endeavoured offering students a high qualitative education integrating hybrid courses based on PBL principles, which consist of on-site (including the transmission of necessary background and practical laboratory training) and off-site (including self-study phases) sessions. As practical laboratory units are central in those courses, the restrictive measures, including the transition to a complete online teaching format due to the first Covid-19-pandemic lock-down, had severe effects on the implementation and the quality of the curriculum. According to surveys made specifically to address this problematic situation, it can be concluded that on-site practical units are fundamental for certain disciplines such as Life Sciences. KW - Problem-based Learning KW - Life Science didactics KW - Hybrid PBL-methods KW - COVID-19 KW - Life Science Education Y1 - VL - 9 IS - 1 SP - 109 EP - 130 ER - TY - CHAP A1 - Mally, Franziska T1 - In-Shoe Plantar Pressure Measurement—Influence of Insole Placement on Selected Parameters during Running. T2 - ISEA 2020 Online - Proceedings 2020, 49(1) KW - Sports Equipment Technologies KW - Running Shoes KW - Motion Analysis Y1 - 2020 SP - 50 EP - 50 ER - TY - CHAP A1 - Wießner, Katharina A1 - Praher, Daniela A1 - Liousia, Varvara A1 - Gepp, Barbara A1 - Leitner, Rita A1 - Rünzler, Dominik T1 - Testing the acute toxicity of an alternative to Glyphosate - Pelargonic acid T2 - SETAC Europe 30th Annual Meeting - Online N2 - Glyphosate-based herbicides were used as plant protection product globally for several decades. However, glyphosate is discussed as showing genotoxicity and many other side-effects such as inhibiting the mitochondrial succinate dehydrogenase, leading to a decreased ATP production. Therefore, finding alternative active substances is necessary. Pelargonic acid (nonanoic acid) and its ammonium salt (saponified form), which are used as alternatives to glyphosate-based herbicides, are biological derived substances considered as environmentally friendly herbicides. To test the effects of pelargonic acid in its acidic form (active substance in TopGun) and its saponified form (in Finalsan Plus) on the aquatic ecosystem, students of the master study program “environmental management and ecotoxicology” compared the toxicity levels of these substances using zebrafish embryos within the scope of a student research course. The project was developed by applying the 7-step problem-based learning method which allowed the students to design their experiments independently with the guidance and feedback of the lecturers. Acute toxicity was determined according to OECD test guideline 236 in D. rerio which revealed a LC50 of 1.55 mg/L of pelargonic acid, a LC50 value of 0.93 mg/L pelargonic acid in TopGun and a LC50 of 36.37 mg/L of Finalsan Plus. Neutral Red Uptake assays were performed on the rainbow trout-derived gill cell-line RTgill-W1 to determine the acute toxicity according to the OECD test guideline 129 which revealed the IC50 value of 12.4 mg/L pelargonic acid in TG. Due to solubility limits, no reliable IC50 could be obtained. The results of the tests indicate differences in the acute toxicity of the pelargonic acid-based formulations TopGun and Finalsan Plus. However, more investigations have to be done in order to analyse if the acidic form is more toxic than the saponified form. Furthermore, detailed ecotoxicological risk assessments and an evaluation of the genotoxicity of both herbicides has to be performed before any conclusion can be drawn. Financial support from the City of Vienna project PBL in Molecular Life Science (21-06) is gratefully acknowledged. KW - Environmental Chemistry KW - Ecotoxicology Y1 - 2020 ER - TY - JOUR A1 - Ziadlou, Reihane A1 - Rotman, Stijn A1 - Teuschl, Andreas A1 - Salzer, Elias A1 - Barbero, Andrea A1 - Martin, Ivan A1 - Alini, Mauro A1 - Eglin, David A1 - Grad, Sibylle T1 - Optimization of hyaluronic acid-tyramine/silk-fibroin composite hydrogels for cartilage tissue engineering and delivery of anti-inflammatory and anabolic drugs JF - Materials Science and Engineering: C N2 - Injury of articular cartilage leads to an imbalance in tissue homeostasis, and due to the poor self-healing capacity of cartilage the affected tissue often exhibits osteoarthritic changes. In recent years, injectable and highly tunable composite hydrogels for cartilage tissue engineering and drug delivery have been introduced as a desirable alternative to invasive treatments. In this study, we aimed to formulate injectable hydrogels for drug delivery and cartilage tissue engineering by combining different concentrations of hyaluronic acid-tyramine (HA-Tyr) with regenerated silk-fibroin (SF) solutions. Upon enzymatic crosslinking, the gelation and mechanical properties were characterized over time. To evaluate the effect of the hydrogel compositions and properties on extracellular matrix (ECM) deposition, bovine chondrocytes were embedded in enzymatically crosslinked HA-Tyr/SF composites (in further work abbreviated as HA/SF) or HA-Tyr hydrogels. We demonstrated that all hydrogel formulations were cytocompatible and could promote the expression of cartilage matrix proteins allowing chondrocytes to produce ECM, while the most prominent chondrogenic effects were observed in hydrogels with HA20/SF80 polymeric ratios. Unconfined mechanical testing showed that the compressive modulus for HA20/SF80 chondrocyte-laden constructs was increased almost 10-fold over 28 days of culture in chondrogenic medium which confirmed the superior production of ECM in this hydrogel compared to other hydrogels in this study. Furthermore, in hydrogels loaded with anabolic and anti-inflammatory drugs, HA20/SF80 hydrogel showed the longest and the most sustained release profile over time which is desirable for the long treatment duration typically necessary for osteoarthritic joints. In conclusion, HA20/SF80 hydrogel was successfully established as a suitable injectable biomaterial for cartilage tissue engineering and drug delivery applications. KW - Tissue Engineering KW - Cartilage KW - Mechanical Testing KW - Biomaterials KW - Chondrocytes Y1 - VL - 120 IS - 111701 ER - TY - JOUR A1 - Frohner, Matthias A1 - Mense, Alexander A1 - Krondraf, Nikolaus A1 - Sabutsch, Stefan T1 - Implementierungsleitfaden HL7 Implementation Guide for CDA® R2: Implementierungsleitfaden Meldung an das Epidemiologische Meldesystem (EMS) – Labor-und Arztmeldung, ELGA GmbH JF - ELGA CDA Implementierungsleitfäden HL7 KW - eHealth KW - ELGA Y1 - VL - 2020 ER - TY - JOUR A1 - Frohner, Matthias A1 - Sabutsch, Stefan A1 - Krondraf, Nikolaus T1 - ELGA CDA Implementierungsleitfäden HL7 Implementation Guide for CDA® R2: Ambulanzbefund, ELGA GmbH JF - ELGA CDA Implementierungsleitfäden HL7 KW - eHealth KW - ELGA Y1 - VL - 2020 ER - TY - CHAP A1 - Eckelt, Markus A1 - Mally, Franziska T1 - Running Shoes—Possible Correlations of Biomechanical and Material Tests. T2 - ISEA 2020 Online - Proceedings 2020, 49(1) KW - Sports Equipment Technologies KW - Running Shoes Y1 - 2020 SP - 25 EP - 25 ER - TY - CHAP A1 - Eckelt, Markus A1 - Mally, Franziska T1 - Use of Acceleration Sensors in Archery T2 - ISEA 2020 Online - Proceedings 2020, 49(1) KW - Sports Equipment Technologies KW - Acceleration Sensors KW - Archery Y1 - 2020 SP - 98 EP - 98 ER - TY - JOUR A1 - Quartinello, Felice A1 - Tallian, Claudia A1 - Auer, Julia A1 - Schön, Herta A1 - Vielnascher, Robert A1 - Weinberger, Simone A1 - Wieland, Karin A1 - Weihs, Anna A1 - Rollett, Alexandra A1 - Lendl, Bernhard A1 - Teuschl, Andreas A1 - Pellis, Alessandro A1 - Gübitz, Georg T1 - Smart Textiles in Wound Care: Functionalization of Cotton/PET Blends with Antimicrobial Nanocapsules JF - Journal of Materials Chemistry B KW - Smart textiles KW - Tissue Engineering KW - Regenerative Medicine KW - Antimicrobial KW - Wound Y1 - 2019 ER - TY - JOUR A1 - Feichtinger, Xaver A1 - Monforte, Xavier A1 - Keibl, Claudia A1 - Hercher, David A1 - Schanda, Jakob A1 - Teuschl, Andreas A1 - Muschitz, Christian A1 - Redl, Heinz A1 - Fialka, Christian A1 - Mittermayr, Rainer T1 - Substantial Biomechanical Improvement by Extracorporeal Shockwave Therapy After Surgical Repair of Rodent Chronic Rotator Cuff Tears. JF - American Journal of Sports Medicine KW - Shockwave Therapy KW - Tissue Engineering KW - Regeneration KW - Surgery Y1 - ER - TY - JOUR A1 - Teuschl, Andreas A1 - Tangl, Stefan A1 - Heimel, Patrick A1 - Schwarze, Uwe Yacine A1 - Monforte, Xavier A1 - Redl, Heinz A1 - Nau, Thomas T1 - Osteointegration of a Novel Silk Fiber-Based ACL Scaffold by Formation of a Ligament-Bone Interface. JF - American Journal of Sports Medicine KW - Tissue Engineering KW - Silk KW - Scaffold KW - ACL Y1 - ER - TY - JOUR A1 - Simsa, Robin A1 - Monforte, Xavier A1 - Salzer, Elias A1 - Teuschl, Andreas A1 - Jenndahl, Lachmi A1 - Bergh, Niklas A1 - Fogelstrand, Per T1 - Effect of fluid dynamics on decellularization efficacy and mechanical properties of blood vessels. JF - PLoS One KW - Tissue Engineering KW - Decellularization KW - Blood Vessel Y1 - ER - TY - JOUR A1 - Jesenberger, Veronika A1 - Zmajkovicova, Katarina A1 - Catalanotti, Federica A1 - Baumgartner, Christian A1 - Reyes, Gloria Ximena A1 - Baccarini, Manuela T1 - MEK1 is required for PTEN membrane recruitment, AKT regulation, and the maintenance of peripheral tolerance JF - Mol. Cell N2 - The Raf/MEK/ERK and PI3K/Akt pathways are prominent effectors of oncogenic Ras. These pathways negatively regulate each other, but the mechanism involved is incompletely understood. We now identify MEK1 as an essential regulator of lipid/protein phosphatase PTEN, through which it controls phosphatidylinositol-3-phosphate accumulation and AKT signaling. MEK1 ablation stabilizes AKT activation and, in vivo, causes a lupus-like autoimmune disease and myeloproliferation. Mechanistically, MEK1 is necessary for PTEN membrane recruitment as part of a ternary complex containing the multidomain adaptor MAGI1. Complex formation is independent of MEK1 kinase activity but requires phosphorylation of T292 on MEK1 by activated ERK. Thus, inhibiting the ERK pathway reduces PTEN membrane recruitment, increasing phosphatidylinositol-3-phosphate accumulation and AKT activation. Our data offer a conceptual framework for the observation that activation of the PI3K pathway frequently mediate resistance to MEK inhibitors and for the promising results obtained by combined MEK/PI3K inhibition in preclinical cancer models. KW - MEK1 pathway KW - membrane recruitment KW - preclinical cancer models Y1 - 2020 VL - 2013 IS - 50 SP - 43 EP - 55 ER - TY - CHAP A1 - Nebel, Sabrina A1 - Salado Manzano, Cristina A1 - Just, Valentin A1 - Leeb, Christine A1 - Jesenberger, Veronika T1 - Role of the MEK/ERK pathway in chondrogenic differentiation: Establishment of a protocol for the generation of MEK1-knockout hTERT ASCs and assessment of their differentiation potential T2 - Proceedings des Forschungsforum 2017 der österreichischen Fachhochschulen KW - Chondrogenesis Y1 - 2018 ER - TY - GEN A1 - Nebel, Sabrina A1 - Salado Manzano, Cristina A1 - Just, Valentin A1 - Leeb, Christine A1 - Jesenberger, Veronika T1 - Role of the MEK/ERK pathway in chondrogenic differentiation: Establishment of a protocol for the generation of MEK1-knockout hTERT ASCs and assessment of their differentiation potential KW - Chondrogenesis Y1 - 2018 ER - TY - CHAP A1 - Leitner, Rita A1 - Gepp, Barbara A1 - Liousia, Varvara A1 - Rünzler, Dominik T1 - Teaching 3R principles in ecotoxicology by performing a problem-based learning project T2 - ALTEX Proceedings, LINZ 2018 – EUSAAT 2018, Volume 7, No. 2, ISSN 2194-0479 (2018) KW - Problem Based Learning KW - Teaching KW - Ecotoxicology KW - 3R Principles Y1 - ER - TY - JOUR A1 - Catalanotti, Federica A1 - Reyes, Gloria Ximena A1 - Jesenberger, Veronika A1 - Galabova-Kovacs, Gergana A1 - de Matos Simoes, Ricardo A1 - Carugo, Oliviero A1 - Baccarini, Manuela T1 - A Mek1-Mek2 heterodimer determines the strength and duration of the Erk signal JF - Nat Struct Mol Biol. N2 - Mek1 and Mek2 (also known as Map2k1 and Map2k2, respectively) are evolutionarily conserved, dual-specificity kinases that mediate Erk1 and Erk2 activation during adhesion and growth factor signaling. Here we describe a previously uncharacterized, unexpected role of Mek1 in downregulating Mek2-dependent Erk signaling. Mek1 mediates the regulation of Mek2 in the context of a previously undiscovered Mek1-Mek2 complex. The Mek heterodimer is negatively regulated by Erk-mediated phosphorylation of Mek1 on Thr292, a residue missing in Mek2. Disabling this Erk-proximal negative-feedback step stabilizes the phosphorylation of both Mek2 and Erk in cultured cells and in vivo in Mek1 knockout embryos and mice. Thus, in disagreement with the current perception of the pathway, the role of Mek1 and Mek2 in growth factor-induced Erk phosphorylation is not interchangeable. Our data establish Mek1 as the crucial modulator of Mek and Erk signaling and have potential implications for the role of Mek1 and Mek2 in tumorigenesis. KW - Mek1-Mek2 pathway KW - tumorigenesis KW - ERK activation KW - cancer biology Y1 - 2020 VL - 2009 IS - 16(3) SP - 294 EP - 303 ER - TY - GEN A1 - Leitner, Rita T1 - PBL-Szenario mit degressivem Support durch Lehrende im Modul "Angewandtes Forschungsprojekt in Umweltmanagement" KW - Problem Based Learning KW - Didactics KW - Environmental Management Y1 - ER - TY - GEN A1 - Gepp, Barbara A1 - Liousia, Varvara A1 - Machacek, Thomas A1 - Praher, Daniela A1 - Eisner, Katharina A1 - Limbeck, Sophie A1 - Gamper, Janine A1 - Schwarzl, Elisabeth A1 - Hazod, Till A1 - Landwehr, Renate A1 - Wenger, Charlotte A1 - Olscher, Christoph A1 - Leitner, Rita A1 - Rünzler, Dominik T1 - Pelargonic acid - an alternative to glyphosate-based herbicides? KW - Ecotoxicology KW - Herbicides Y1 - ER - TY - GEN A1 - Leitner, Rita T1 - Teaching 3R principles in ecotoxicology by performing a problem-based learning project KW - Problem Based Learning KW - Teaching KW - Ecotoxicology KW - Education KW - 3R Principles Y1 - ER - TY - GEN A1 - Bayer, Natascha T1 - Intraocular lens measurement setup based on a mechanical eye model KW - Eye Model KW - Intraocular Lens KW - Cataract Y1 - ER - TY - GEN A1 - Küenburg, Bernhard A1 - Teuschl, Andreas T1 - Regenerative silk ligament: scale up and regulatory strategy of a textile engineered silk implant for tissue regeneration of injured human ACL (anterior cruciate ligament) N2 - 61 a93591 27-SY-1 Regenerative silk ligament: scale up and regulatory strategy of a textile engineered silk implant for tissue regeneration of injured human ACL (anterior cruciate ligament) Bernhard Küenburg，Andreas Teuschl MorphoMed, Austria In the USA around 370.000 annual ACL ruptures in the predominately young population (age 18-30) following sport injuries cause a lot of pain and long term troubles for the affected patients. The well established standard procedure of autologous tissue transplantation stabilizes the knee and allows sport activities after 9-12 months. However, harvest of autologous tissue such as patellar tendon or semitendinosus (the most frequently used autograft) weakens other body areas and long term data show an increased rate of arthroses (up to 50% after 10 years) associated with ACL reconstructions. Based on numerous preclinical data and data from a 12-month sheep study the scale-up as well as regulatory approval process of a novel textile engineered silk implant as ACL graft have been initiated. It is planned to enter a clinical trial and strive for regulatory approval as a medical class 3 implant. The sheep data (1) have revealed an approximately 50% degradation of the purified medical silk, which acts as scaffold for the regrowth of a new endogenous ligament. The formation of oriented collagenous tissue fibers including vascularization proves a ligament tissue regeneration for the first time. Scale-up and implementation of a commercial process require a defined set of specifications of commercial silkworm (Bombyx mori) silk, a biological raw material sourced from the textile industry as well as adequate analytical methods to characterize the depletion of sericin in the course of the production process. Based on first full scale samples, the defined biocompatibility program has to be executed, in the EU following the ISO requirements of the notified bodies, in the USA determined by the FDA. Upon submission of the full technical documentation as well as the biocompati-bility data, approval of a clinical trial can be achieved in order to demonstrate the clinical efficacy of the silk based ACL graft compared to the gold standard method in two different randomized groups. As primary endpoint the knee stability will be tested by an apparatus supported Lachmann test. In this study, besides the clinical benefit the patient safety is an important goal. The results of this study will be used for achievement of regulatory approval. References: 1) Teuschl A, Heimel P, Nürnberger S, van Griensven M, Redl H, Nau T. Am. J. Sports Med. 2016;44:1547–57. 2) Teuschl AH, van Griensven M, Redl H. Tissue Eng. Part C Methods. 2014;20:431–9. KW - Biomaterial KW - Ligament Y1 - ER - TY - JOUR A1 - Tallian, Claudia A1 - Herrero-Rollett, Alexandra A1 - Stadler, Karina A1 - Vielnascher, Robert A1 - Wieland, Karin A1 - Weihs, Anna A1 - Pellis, Alessandro A1 - Teuschl, Andreas A1 - Lendl, Bernhard A1 - Amenitsch, Heinz A1 - Guebitz, Georg M. T1 - Structural insights into pH-responsive drug release of self-assembling human serum albumin-silk fibroin nanocapsules. JF - European Journal of Pharmaceutics and Biopharmaceutics N2 - Inflammation processes are associated with significant decreases in tissue or lysosomal pH from 7.4 to 4, a fact that argues for the application of pH-responsive drug delivery systems. However, for their design and optimization a full understanding of the release mechanism is crucial. In this study we investigated the pH-depending drug release mechanism and the influence of silk fibroin (SF) concentration and SF degradation degree of human serum albumin (HSA)-SF nanocapsules. Sonochemically produced nanocapsules were investigated regarding particle size, colloidal stability, protein encapsulation, thermal stability and drug loading properties. Particles of the monodisperse phase showed average hydrodynamic radii between 438 and 888 nm as measured by DLS and AFM and a zeta potential of -11.12 ± 3.27 mV. Together with DSC results this indicated the successful production of stable nanocapsules. ATR-FTIR analysis demonstrated that SF had a positive effect on particle formation and stability due to induced beta-sheet formation and enhanced crosslinking. The pH-responsive release was found to depend on the SF concentration. In in-vitro release studies, HSA-SF nanocapsules composed of 50% SF showed an increased pH-responsive release for all tested model substances (Rhodamine B, Crystal Violet and Evans Blue) and methotrexate at the lowered pH of 4.5 to pH 5.4, while HSA capsules without SF did not show any pH-responsive drug release. Mechanistic studies using confocal laser scanning microscopy (CLSM) and small angle X-ray scattering (SAXS) analyses showed that increases in particle porosity and decreases in particle densities are directly linked to pH-responsive release properties. Therefore, the pH-responsive release mechanism was identified as diffusion controlled in a novel and unique approach by linking scattering results with in vitro studies. Finally, cytotoxicity studies using the human monocytic THP-1 cell line indicated non-toxic behavior of the drug loaded nanocapsules when applied in a concentration of 62.5 µg mL-1. KW - Biomaterial KW - Tissue Engineering Y1 - ER - TY - GEN A1 - Teuschl, Andreas A1 - Schuh, Christina A1 - Weihs, Anna A1 - Guillaume, Olivier A1 - Monforte Vila, Xavier A1 - Redl, Heinz A1 - Kaplan, David A1 - Rünzler, Dominik T1 - Tailoring bioactivity of silk-based biomaterials via delivering and functionalization strategies with fibrinogen/thrombin, plant lectins or laminin KW - Biomaterials KW - Tissue Engineering KW - Silk Y1 - ER - TY - JOUR A1 - Slezak, Paul A1 - Slezak, Cyrill A1 - Hartinger, Joachim A1 - Teuschl, Andreas A1 - Nürnberger, Sylvia A1 - Redl, Heinz A1 - Mittermayr, Rainer T1 - A Low Cost Implantation Model in the Rat That Allows a Spatial Assessment of Angiogenesis. JF - Frontiers in Bioengineering and Biotechnology N2 - There is continual demand for animal models that allow a quantitative assessment of angiogenic properties of biomaterials, therapies, and pharmaceuticals. In its simplest form, this is done by subcutaneous material implantation and subsequent vessel counting which usually omits spatial data. We have refined an implantation model and paired it with a computational analytic routine which outputs not only vessel count but also vessel density, distribution, and vessel penetration depth, that relies on a centric vessel as a reference point. We have successfully validated our model by characterizing the angiogenic potential of a fibrin matrix in conjunction with recombinant human vascular endothelial growth factor (rhVEGF165). The inferior epigastric vascular pedicles of rats were sheathed with silicone tubes, which were subsequently filled with 0.2 ml of fibrin and different doses of rhVEGF165, centrically embedding the vessels. Over 4 weeks, tissue samples were harvested and subsequently immunohistologically stained and computationally analyzed. The model was able to detect variations over the angiogenic potentials of growth factor spiked fibrin matrices. Adding 20 ng of rhVEGF165 resulted in a significant increase in vasculature while 200 ng of rhVEGF165 did not improve vascular growth. Vascularized tissue volume increased during the first week and vascular density increased during the second week. Total vessel count increased significantly and exhibited a peak after 2 weeks which was followed by a resorption of vasculature by week 4. In summary, a simple implantation model to study in vivo vascularization with only a minimal workload attached was enhanced to include morphologic data of the emerging vascular tree. KW - Tissue Engineering KW - Bioreactor KW - Biomaterial Y1 - ER - TY - JOUR A1 - Berkovitch, Yulia A1 - Cohen, Talia A1 - Peled, Eli A1 - Schmidhammer, Robert A1 - Hildner, Florian A1 - Teuschl, Andreas A1 - Wolbank, Susanne A1 - Yelin, Dvir A1 - Redl, Heinz A1 - Seliktar, Dror T1 - Hydrogel composition and laser micropatterning to regulate sciatic nerve regeneration. JF - Journal of Tissue Engineering and Regenerative Medicine N2 - Treatment of peripheral nerve injuries has evolved over the past several decades to include the use of sophisticated new materials endowed with trophic and topographical cues that are essential for in vivo nerve fibre regeneration. In this research, we explored the use of an advanced design strategy for peripheral nerve repair, using biological and semi-synthetic hydrogels that enable controlled environmental stimuli to regenerate neurons and glial cells in a rat sciatic nerve resection model. The provisional nerve growth conduits were composed of either natural fibrin or adducts of synthetic polyethylene glycol and fibrinogen or gelatin. A photo-patterning technique was further applied to these 3D hydrogel biomaterials, in the form of laser-ablated microchannels, to provide contact guidance for unidirectional growth following sciatic nerve injury. We tested the regeneration capacity of subcritical nerve gap injuries in rats treated with photo-patterned materials and compared these with injuries treated with unpatterned hydrogels, either stiff or compliant. Among the factors tested were shear modulus, biological composition, and micropatterning of the materials. The microchannel guidance patterns, combined with appropriately matched degradation and stiffness properties of the material, proved most essential for the uniform tissue propagation during the nerve regeneration process. KW - Tissue Engineering KW - Biomaterials KW - Nerve Regeneration Y1 - SP - 1049 EP - 1061 ER - TY - JOUR A1 - Nau, Thomas A1 - Teuschl, Andreas A1 - Ebner, Anna A1 - Jung, Ilse A1 - Schenk, Christian T1 - Low revision rate and excellent outcome of primary ACL repair with a minimum follow-up of 5 years. JF - Muscle, ligaments and tendons Journal N2 - Introduction: Due to limitations of ACL reconstruction, primary ACL repair has recently regained research interest. Although abandoned in the past, primary repair with conservation of the original ligament demonstrates considerable advantages compared to reconstruction. We hypothesized that early repair, strictly limited to patients with a proximal ACL rupture and excellent tissue quality of the remaining ACL stump, would lead to equal revision rates and subjective outcomes as reported for ACL reconstruction after a minimum of 5 years. Methods: In this questionnaire study, patients who had a primary ACL repair between 2002 and 2009 were invited to participate. Besides any potential revision surgery, the Tegner activity scale and the Knee Injury and Osteoarthritis Outcome Score were included in the evaluation. Results: Out of 1912 patients who had ACL related surgery during the observation period, 221 (11.4%) had a primary ACL repair. 60 patients (61 knees) were available for follow-up. In 2/61 (3.3%) cases ACL revision surgery was performed and one patient had meniscus surgery of the affected side. The median Tegner activity scale was 6 (range, 3 to 10). The mean KOOS subscores were 88.8% (Function/Sports), 86.6% (Quality of life), 94.6 (Symptoms), 94.0 (Pain) and 97.0 (Activities of Daily Living). Conclusion: Primary ACL repair, strictly limited to proximal ruptures with good tissue quality leads to revision rates and subjective outcome comparable to ACL reconstruction. Level of evidence: IV. KW - Regeneration Y1 - SP - 185 EP - 190 ER - TY - GEN A1 - Teuschl, Andreas A1 - Heimel, Patrick A1 - Monforte Vila, Xavier A1 - Nürnberger, Sylvia A1 - Tangl, Stefan A1 - van Griensven, Martijn A1 - Redl, Heinz A1 - Nau, Thomas T1 - Anterior cruciate ligament regeneration using the silk-based RegACL scaffold KW - Tissue Engineering KW - Silk KW - Biomaterial KW - Anterior Cruciate Ligament Y1 - ER - TY - CHAP A1 - Hackethal, Johannes A1 - Schuh, Christina A1 - Hofer, Alexandra A1 - Meixner, Barbara A1 - Hennerbichler, Simone A1 - Redl, Heinz A1 - Teuschl, Andreas T1 - Human Placenta Laminin-111 as a Multifunctional Protein for Tissue Engineering and Regenerative Medicine T2 - Advances in Experimental Medicine and Biology KW - Biomaterial KW - Tissue Engineering KW - Regenerative medicine Y1 - PB - Springer ER - TY - GEN A1 - Salzer, Elias A1 - Rieder, Bernhard A1 - Monforte Vila, Xavier A1 - Weihs, Anna A1 - Rünzler, Dominik A1 - Teuschl, Andreas T1 - Evaluation of a novel hydrostatic pressure bioreactor on bovine cartilage chips KW - Bioreactor KW - Tissue Engineering KW - Cartilage Y1 - ER - TY - GEN A1 - Slezak, Paul A1 - Rose, Roland A1 - Hercher, David A1 - Weihs, Anna A1 - Fuchs, Christiane A1 - Redl, Heinz A1 - Mittermayr, Rainer A1 - Slezak, Cyrill T1 - Tracking therapeutic shockwaves and their impact on regeneration KW - Shockwave Therapy KW - Tissue Regeneration Y1 - ER - TY - GEN A1 - Drauschke, Andreas T1 - Neues aus der Welt der Optik - von Laserschwertern und Tarnvorrichtungen KW - Optics Y1 - ER - TY - GEN A1 - Drauschke, Andreas T1 - Effiziente Solarzellen mit Metamaterialien KW - Photonics Y1 - ER - TY - CHAP A1 - Tkachenko Bril, Andrés Igor A1 - Jagos, Harald A1 - David, Veronika A1 - Pils, Katharina A1 - Gaudernak, Jakob A1 - Rafolt, Dietmar ED - Schreier, Günter ED - Hayn, Dieter T1 - Proof of Concept of a Partial Weight-Bearing Supporting Real-Time Feedback System T2 - Health Informatics meets eHealth - Proceedings of the 12th eHealth Conference KW - Ambulation KW - Weight-Bearing KW - Patient KW - Feedback Y1 - PB - IOS Press CY - Amsterdam ER - TY - CHAP A1 - Franzl, Gerald A1 - Reif, Valerie A1 - Berger, Angela A1 - Frohner, Matthias A1 - Gottschalk, Marion A1 - Koch, Georg T1 - Interoperabilität im Datenaustausch in der Energiewirtschaft vom Use Case zum Test der Integrationsprofile T2 - 15. Symposium Energieinnovation KW - Interoperability KW - Virtual Power Plant KW - Smart Grid KW - Power Supply Y1 - ER - TY - GEN A1 - Eisner, Katharina A1 - Gepp, Barbara A1 - Schnegg, Raimund A1 - Dallinger, Reinhard A1 - Rünzler, Dominik T1 - Investigation of endocrine effects of sublethal cadmium doses on the reproduction system of the freshwater snail B. glabrata KW - Ecotoxicology KW - Endocrine disruptors Y1 - ER - TY - GEN A1 - Franzl, Gerald A1 - Reif, Valerie A1 - Berger, Angela A1 - Frohner, Matthias A1 - Gottschalk, Marion A1 - Koch, Georg T1 - Interoperabilität im Datenaustausch in der Energiewirtschaft vom Use Case zum Test der Integrationsprofile KW - Interoperability KW - Virtual Power Plant KW - Smart Grid KW - Power Supply Y1 - ER - TY - GEN A1 - Tkachenko Bril, Andrés Igor A1 - Jagos, Harald A1 - David, Veronika A1 - Pils, Katharina A1 - Gaudernak, Jakob A1 - Rafolt, Dietmar T1 - Proof of Concept of a Partial Weight-Bearing Supporting Real-Time Feedback System KW - Ambulation KW - Weight-Bearing KW - Patient KW - Feedback Y1 - ER - TY - GEN A1 - Salzer, Elias A1 - Liousia, Varvara A1 - Monforte Vila, Xavier A1 - Rünzler, Dominik T1 - Tracking of small aquatic organisms with custom-made tracking plates KW - Ecotoxicology KW - Behavior Analysis KW - Aquatic Ecotoxicology Y1 - ER - TY - GEN A1 - Wilfing, Julia A1 - Frühwirth, Clemens A1 - Lehner, Benjamin T1 - Feldmessungen der Gleit- und Haftreibungskoeffizienten von Skitourenfellen KW - Skiing KW - Measuring KW - Friction Y1 - ER - TY - GEN A1 - Drauschke, Andreas T1 - Applications of Optogenetics KW - Optogenetics Y1 - 2018 ER - TY - JOUR A1 - Hackethal, Johannes A1 - Mühleder, Severin A1 - Hofer, Alexandra A1 - Schneider, Karl Heinrich A1 - Prüller, Johanna A1 - Hennerbichler, Simone A1 - Redl, Heinz A1 - Teuschl, Andreas T1 - An Effective Method of Atelocollagen Type 1/3 Isolation from Human Placenta and Its In Vitro Characterization in Two-Dimensional and Three-Dimensional Cell Culture Applications JF - Tissue Eng Part C Methods KW - Placenta KW - In Vitro KW - Cell Culture Y1 - 2018 VL - 23 IS - 5 SP - 274 EP - 285 ER - TY - CHAP A1 - Fuchs, Christiane A1 - Weihs, Anna A1 - Szwarc, Dorota A1 - Mittermayr, Rainer A1 - Rünzler, Dominik A1 - Teuschl, Andreas T1 - Shock wave treatment of muscle (stem) cells - a new implementation for regeneration T2 - Proceedings of the 20th International Congress of the ISMST KW - Shockwave treatment KW - Muscle Cells KW - Regeneration Y1 - 2018 ER - TY - GEN A1 - Veigl, Christoph A1 - David, Veronika T1 - AsTeRICS und FlipMouse: Flexible Eingabesysteme und kostengünstige Lösungen für Kommunikation und Umgebungssteuerung für Menschen mit Behinderung KW - Communication KW - Assistive Technology KW - AsTeRICS framework Y1 - ER - TY - RPRT A1 - Freistetter, Florian T1 - The Power of Lauf KW - Bioreactor KW - Muscle KW - Tissue Engineering Y1 - ER - TY - JOUR A1 - Berkovitch, Yulia A1 - Cohen, Talia A1 - Peled, Eli A1 - Schmidhammer, Robert A1 - Hildner, Florian A1 - Teuschl, Andreas A1 - Wolbank, Susanne A1 - Yelin, Dvir A1 - Redl, Heinz A1 - Seliktar, Dror T1 - Hydrogel Composition and Laser Micro-Patterning to Regulate Sciatic Nerve Regeneration JF - Journal of Tissue Engineering and Regenerative Medicine KW - Micro-Patterning KW - Laser KW - Nerve Regeneration Y1 - 2018 VL - 12 IS - 4 ER - TY - GEN A1 - Fuchs, Christiane A1 - Weihs, Anna A1 - Szwarc, Dorota A1 - Mittermayr, Rainer A1 - Rünzler, Dominik A1 - Teuschl, Andreas T1 - Shock wave treatment of muscle (stem) cells - a new implementation for regeneration KW - Shockwave treatment KW - Muscle Cells KW - Regeneration Y1 - 2018 ER - TY - GEN A1 - Teuschl, Andreas A1 - Weihs, Anna A1 - Fuchs, Christiane A1 - Monforte Vila, Xavier T1 - Silk as a versatile biomaterial for musculoskeletal tissue engineering KW - Silk KW - Biomaterials Y1 - 2018 ER - TY - GEN A1 - Szwarc, Dorota A1 - Fuchs, Christiane A1 - Weihs, Anna A1 - Rünzler, Dominik T1 - Molecular mechanisms underlying the potential of shock wave treatment for cardiac therapy KW - Shockwave treatment KW - Cardiac Y1 - 2018 ER - TY - GEN A1 - Szwarc, Dorota A1 - Fuchs, Christiane A1 - Purtscher, Michaela A1 - Rünzler, Dominik T1 - Elucidating the molecular mechanisms underlying cardiac shock wave therapy KW - Shockwave treatment KW - Cardiac Y1 - 2018 ER - TY - GEN A1 - Sabo, Anton A1 - Eckelt, Markus T1 - Running Shoes - Possible Correlations of Biomechanical and Material Tests KW - Shoes KW - Testing Y1 - ER - TY - GEN A1 - Eckelt, Markus T1 - Customizing Shoe Insoles through CAD-FEM Modelling KW - Customized Equipment KW - Arch ratio KW - Footwear design KW - Contact pressure KW - 3D Anthropometry Y1 - ER - TY - JOUR A1 - Bernhard, Jonathan A1 - Ferguson, James A1 - Rieder, Bernhard A1 - Heimel, Patrick A1 - Nau, Thomas A1 - Tangl, Stefan A1 - Redl, Heinz A1 - Vunjak-Novakovic, Gordana T1 - Tissue-engineered hypertrophic chondrocyte grafts enhanced long bone repair biomaterials JF - Biomaterials KW - Grafting KW - Biomaterials Y1 - 2018 IS - 139 SP - 202 EP - 212 ER - TY - GEN A1 - Kotzian, Stefan A1 - Riess, Bernhard A1 - David, Veronika A1 - Haider, Sandra A1 - Spatt, Josef T1 - Virtual Reality Training in der neurologischen Rehabilitation - Eine Praktikabilitätsstudie zu einer Microsoft Kinect-Anwendung KW - Neurology KW - Rehabilitation KW - Virtual Reality Y1 - 2018 ER - TY - GEN A1 - Menschik, Alfred A1 - Mally, Franziska A1 - Gruber, Martin A1 - Sabo, Anton T1 - EMG-Messungen am Unterwasserlaufband KW - Electromyography KW - Health Y1 - 2018 ER - TY - GEN A1 - Bayer, Natascha T1 - Analysing the Impact of a Misaligned Toric Intraocular Lens on Wave Front Aberrations KW - Mechanical Eye Y1 - 2018 ER - TY - GEN A1 - Rünzler, Dominik T1 - Alternatives to Animal Experiment Models: Tracking Zebrafish Larvae and Daphnia KW - Animal Experiments KW - Zebrafish Y1 - 2018 ER - TY - GEN A1 - Eckelt, Markus T1 - Evaluating the Interaction Between Rim and Brake Pad on a Test Bench KW - Brake KW - Wheels KW - Test Bench Y1 - ER - TY - GEN A1 - Fuchs, Christiane T1 - The importance of mechanotransduction in myogenesis KW - Mechanotransduction KW - Myogenesis Y1 - 2018 ER - TY - JOUR A1 - Schneider, Cornelia A1 - Lehmann, Johannes A1 - van Osch, Gerjo A1 - Hildner, Florian A1 - Teuschl, Andreas A1 - Monforte Vila, Xavier A1 - Miosga, David A1 - Heimel, Patrick A1 - Priglinger, Eleni A1 - Redl, Heinz A1 - Wolbank, Susanne A1 - Nürnberger, Sylvia T1 - Systematic Comparison of Protocols for the Preparation of Human Articular Cartilage for Use as Scaffold Material in Cartilage Tissue Engineering JF - Tissue Eng Part C Methods KW - Cartilage KW - Scaffold Material Y1 - 2018 VL - 22 IS - 12 ER - TY - CHAP A1 - Maleiner, Babette A1 - Heher, Philipp A1 - Teuschl, Andreas A1 - Redl, Heinz A1 - Rünzler, Dominik A1 - Fuchs, Christiane T1 - Generation of aligned skeletal muscle-like tissue based on the application of strain to a 3D fibrin scaffold T2 - Proceedings of PACT "Designer Cells go Clinical" Symposium KW - Tissue Generation KW - Fibrin KW - Scaffold Y1 - 2018 ER - TY - GEN A1 - Szwarc, Dorota A1 - Fuchs, Christiane A1 - Weihs, Anna A1 - Monforte Vila, Xavier A1 - Hanetseder, Dominik A1 - Teuschl, Andreas A1 - Rünzler, Dominik T1 - The effect of shock waves on in vitro cartilage development in silk scaffolds KW - Shockwave treatment KW - In Vitro KW - Cartilage KW - Silk Y1 - 2018 ER - TY - GEN A1 - Maleiner, Babette A1 - Heher, Philipp A1 - Teuschl, Andreas A1 - Redl, Heinz A1 - Rünzler, Dominik A1 - Fuchs, Christiane T1 - Generation of aligned skeletal muscle-like tissue based on the application of strain to a 3D fibrin scaffold KW - Tissue Generation KW - Fibrin KW - Scaffold Y1 - 2018 ER - TY - GEN A1 - Teuschl, Andreas A1 - Fuchs, Christiane A1 - Weihs, Anna A1 - Heimel, Patrick A1 - Rünzler, Dominik A1 - Redl, Heinz A1 - Nau, Thomas T1 - The Silk Road from Textiles to Novel Medical Implants KW - Silk Y1 - 2018 ER - TY - GEN A1 - Teuschl, Andreas A1 - Heimel, Patrick A1 - Nürnberger, Sylvia A1 - Redl, Heinz A1 - Nau, Thomas T1 - ACL Regeneration using a novel silk fiber based scaffold - Histological Results of a Large Animal Study KW - Tissue Regeneration KW - Silk KW - Scaffold Y1 - 2018 ER - TY - GEN A1 - Teuschl, Andreas T1 - Silk Processing - from Gels to ACL Replacement KW - Silk Y1 - 2018 ER - TY - CHAP A1 - Fuchs, Christiane A1 - Szwarc, Dorota A1 - Weihs, Anna A1 - Rünzler, Dominik T1 - Shock wave treatment of 3D cardiac model systems activates ERK 1/2 signaling pathway and influences cardiomyogenesis T2 - Proceedings of PACT "Designer Cells go Clinical" Symposium KW - Shockwave treatment KW - Cardiac Y1 - 2018 ER - TY - CHAP A1 - Fuchs, Christiane A1 - Szwarc, Dorota A1 - Weihs, Anna A1 - Rünzler, Dominik T1 - Shock wave treatment of 3D cardiac model systems activates ERK 1/2 signaling pathway and influences cardiomyogenesis T2 - Proceedings of LBG Meeting for Health Sciences 2016 KW - Shockwave treatment KW - Cardiac Y1 - 2018 ER - TY - GEN A1 - Fuchs, Christiane A1 - Szwarc, Dorota A1 - Weihs, Anna A1 - Rünzler, Dominik T1 - Shock wave treatment of 3D cardiac model systems activates ERK 1/2 signaling pathway and influences cardiomyogenesis KW - Shockwave treatment KW - Cardiac Y1 - 2018 ER - TY - CHAP A1 - Fuchs, Christiane A1 - Szwarc, Dorota A1 - Weihs, Anna A1 - Rünzler, Dominik T1 - Shock wave treatment positively influences cardiomyogenesis in an energy-dependent manner T2 - Proceedings of PACT "Designer Cells go Clinical" Symposium KW - Shockwave treatment KW - Cardiac Y1 - 2018 ER - TY - GEN A1 - Weihs, Anna T1 - Shock wave treatment for in vitro tissue engineering applications KW - Shockwave treatment KW - In Vitro KW - Tissue Engineering Y1 - 2018 ER - TY - CHAP A1 - Basoli, Valentina A1 - Chaudry, Sidrah A1 - Cruciani, Sara A1 - Fuchs, Christiane A1 - Rieger, Sabine A1 - Dungel, Peter A1 - Wolbank, Susanne A1 - Ventura, Carlo A1 - Grillari-Voglauer, Regina A1 - Redl, Heinz A1 - Maioli, Margherita T1 - Epigenetic and molecular behavious of stem cells exposed to biophysical stimuli: new insights in regenerative medicine T2 - Proceedings des Seminars zum 40. Jahresjubiläum der Österreichischen Gesellschaft für Chirurgische Forschung KW - Stem Cells KW - Regeneration KW - Biophysical Stimuli Y1 - 2018 ER - TY - GEN A1 - Basoli, Valentina A1 - Chaudry, Sidrah A1 - Cruciani, Gabriele A1 - Fuchs, Christiane A1 - Rieger, Sabine A1 - Dungel, Peter A1 - Wolbank, Susanne A1 - Ventura, Carlo A1 - Grillari-Voglauer, Regina A1 - Redl, Heinz A1 - Maioli, Margherita T1 - Epigenetic and molecular behavious of stem cells exposed to biophysical stimuli: new insights in regenerative medicine KW - Stem Cells KW - Regeneration KW - Biophysical Stimuli Y1 - 2018 ER - TY - GEN A1 - Hofstätter, Otto A1 - Breyer, Peter A1 - Kafka, Patricia A1 - Sabo, Anton T1 - Development of a prototype for the simulation of human sway to make standardized and reproducible measurements of force and pressure sensors KW - Simulation KW - Human Sway Y1 - 2018 ER - TY - GEN A1 - Höttinger, Hannes A1 - Mally, Franziska A1 - Sabo, Anton T1 - Activity recognition in surfing - a comparative study between hidden markov model and support vector machine KW - Activity KW - Surfing Y1 - 2018 ER - TY - CHAP A1 - Drauschke, Andreas T1 - Comparison of Numerical Eye Models and its Representation within a Mechanical Eye Model T2 - IFAC-Papers Online KW - Mechanical Eye Y1 - 2018 SP - 133 EP - 138 ER - TY - GEN A1 - Drauschke, Andreas T1 - Comparison of Numerical Eye Models and its Representation within a Mechanical Eye Model KW - Mechanical Eye Y1 - 2018 ER - TY - GEN A1 - Weihs, Anna T1 - Shock wave treatment for in vitro tissue engineering applications KW - Shockwave treatment KW - In Vitro KW - Tissue Engineering Y1 - 2018 ER - TY - GEN A1 - Weihs, Anna T1 - Mechanotransduction in shock wave treatment KW - Shockwave treatment KW - Mechanotransduction Y1 - 2018 ER - TY - GEN A1 - Mandt, Denise A1 - Pichler, Verena A1 - Melchior, Sophie A1 - Rünzler, Dominik T1 - Establishment of 2D and 3D cell culture assays using RT-gillW1 cells KW - Cell Culture KW - Assay Y1 - 2018 ER - TY - GEN A1 - Öttl, Georg A1 - Mally, Franziska A1 - Sabo, Anton T1 - The influence of low-friction quickdraws on impact forces in climbing falls KW - Climbing KW - Falling Y1 - 2018 ER - TY - GEN A1 - Salzer, Elias A1 - Rünzler, Dominik T1 - Acute Immobilization Test with Daphnia magna - A Refined Methodology for the Detection of Immobilization KW - Immobilization Y1 - 2018 ER - TY - GEN A1 - Bayer, Natascha T1 - Analysis of Customized Ocular Data and Wave Front Aberrations Concerning their Deviations from Generic Eye Models KW - Mechanical Eye Y1 - 2018 ER - TY - GEN A1 - Salzer, Elias A1 - Rünzler, Dominik T1 - Live or dead is not enough: a large-scale mobility assay with Daphnia magna KW - Mobility Assay Y1 - 2018 ER - TY - GEN A1 - Fuchs, Christiane T1 - A mechanical stimulation strategy for engineering skeletal muscle-like tissue on a strained fibrin scaffold KW - Muscle Tissue KW - Fibrin KW - Scaffold Y1 - 2018 ER - TY - GEN A1 - Frohner, Matthias T1 - How to Design Mobile Applications for Security, Safety and Usability KW - Electronic Health Records Y1 - 2018 ER - TY - GEN A1 - Fuchs, Christiane T1 - The beneficial effects of in vitro shock wave treatment on cardiomyogenesis are energy dependent KW - In Vitro KW - Shockwave treatment KW - Myogenesis Y1 - 2018 ER - TY - GEN A1 - David, Veronika A1 - Aigner, Benjamin T1 - AsTeRICS Framework KW - Cross-Cultural Education Y1 - 2018 ER - TY - GEN A1 - Fuchs, Christiane T1 - Mechanotransduction - bioreactors KW - Bioreactors Y1 - 2018 ER - TY - JOUR A1 - Heher, Philipp A1 - Maleiner, Babette A1 - Prüller, Johanna A1 - Teuschl, Andreas A1 - Kollmitzer, Josef A1 - Monforte Vila, Xavier A1 - Wolbank, Susanne A1 - Redl, Heinz A1 - Rünzler, Dominik A1 - Fuchs, Christiane T1 - A novel bioreactor for the generation of highly aligned 3D skeletal muscle-like constructs through orientation of fibrin via application of static strain JF - Acta Biomaterialia KW - Bioreactor Y1 - ER - TY - JOUR A1 - Teuschl, Andreas A1 - Schuh, Christina A1 - Halbweis, Robert A1 - Pajer, Krisztian A1 - Marton, Gabor A1 - Hopf, Rudolf A1 - Mosia, Shorena A1 - Rünzler, Dominik A1 - Redl, Heinz A1 - Nogradi, Antal A1 - Hausner, Thomas T1 - A new preparation method for anisotropic silk fibroin nerve guidance conduits and its evaluation in vitro and in a rat sciatic nerve defect model JF - Tissue Engineering Part C: Methods KW - Fibrin KW - Nerve Defect Y1 - 2018 ER - TY - GEN A1 - Teuschl, Andreas A1 - Schuh, Christina A1 - Halbweis, Robert A1 - Marton, Gábor A1 - Pajer, Krisztián A1 - Hopf, Rudolf A1 - Mosia, Shorena A1 - Rünzler, Dominik A1 - Nógrádi, Antal A1 - Hausner, Thomas A1 - Redl, Heinz T1 - Silk fibroin for peripheral nerve regeneration: a novel preparation method improved mechanical characteristics and supports regeneration in rat sciatic nerves KW - Fibroin KW - Regeneration Y1 - 2018 ER - TY - GEN A1 - Fuchs, Christiane A1 - Weihs, Anna A1 - Teuschl, Andreas A1 - Hartinger, Joachim A1 - Slezak, Paul A1 - Mittermayr, Rainer A1 - Redl, Heinz A1 - Junger, Wolfgang A1 - Sitte, Harald A1 - Rünzler, Dominik T1 - Shockwave Treatment Enhances Proliferation and Improves Wound Healing via Purinergic Signaling Linked ERK 1/2 Pathways KW - Shockwave treatment KW - Healing Processes KW - Signaling Y1 - 2018 ER - TY - GEN A1 - Fuchs, Christiane A1 - Weihs, Anna A1 - Teuschl, Andreas A1 - Hartinger, Joachim A1 - Slezak, Paul A1 - Mittermayr, Rainer A1 - Redl, Heinz A1 - Junger, Wolfgang A1 - Sitte, Harald A1 - Rünzler, Dominik T1 - Shockwave Treatment Augments Proliferation and Improves Wound Healing via Purinergic Signaling Linked ERK 1/2 Pathways KW - Shockwave treatment KW - Healing Processes KW - Signaling Y1 - 2018 ER - TY - GEN A1 - Heher, Philipp A1 - Fuchs, Christiane A1 - Prüller, Johanna A1 - Babette, Maleiner A1 - Rünzler, Dominik A1 - Redl, Heinz T1 - A novel bioreactor for engineering skeletal muscle-like tissue in a strained fibrin scaffold KW - Bioreactor KW - Scaffold KW - Muscle Tissue Y1 - ER - TY - JOUR A1 - Guillaume, Olivier A1 - Park, Jaesung A1 - Monforte Vila, Xavier A1 - Gruber-Blum, Simone A1 - Redl, Heinz A1 - Petter-Puchner, Akexander A1 - Teuschl, Andreas T1 - Fabrication of silk mesh with enhanced cytocompatibility: preliminary in vitro investigation toward cell-based therapy for hernia repair JF - Journal of Materials Science: Materials in Medicine KW - Cytocompatibility KW - Hernia Repair Y1 - 2018 ER - TY - GEN A1 - Weihs, Anna A1 - Fuchs, Christiane A1 - Teuschl, Andreas A1 - Hartinger, Joachim A1 - Slezak, Paul A1 - Mittermayr, Rainer A1 - Redl, Heinz A1 - Junger, Wolfgang A1 - Sitte, Harald A1 - Rünzler, Dominik T1 - Shockwave treatment activates Erk1/2 pathways predominantly via P2Y receptor involvement KW - Shockwave Y1 - ER - TY - JOUR A1 - Teuschl, Andreas A1 - Heimel, Patrick A1 - Nürnberger, Sylvia A1 - van Griensven, Martijn A1 - Redl, Heinz A1 - Nau, Thomas T1 - A Novel Silk Fiber-Based Scaffold for Regeneration of the Anterior Cruciate Ligament: Histological Results From a Study in Sheep. JF - The American Journal of Sports Medicine KW - Ligament KW - Regeneration Y1 - 2018 ER - TY - GEN A1 - Teuschl, Andreas A1 - Fuchs, Christiane A1 - Feichtinger, Georg A1 - Heher, Philipp A1 - Heimel, Patrick A1 - Schuh, Christina A1 - Nürnberger, Sylvia A1 - Nau, Thomas A1 - Rünzler, Dominik A1 - Redl, Heinz T1 - Fibrin or Fibroin - Not only the "o" Makes the Difference KW - Fibrin KW - Fibroin Y1 - 2018 ER - TY - JOUR A1 - Teuschl, Andreas A1 - Balmayor, Elizabeth A1 - Redl, Heinz A1 - van Griensven, Martijn A1 - Dungel, Peter T1 - Phototherapy With LED Light Modulates Healing Processes in an In Vitro Scratch Wound-Model Using 3 Different Cell Types JF - Dermatologic Surgery KW - Phototherapy KW - Healing Processes Y1 - VL - 41 IS - 2 SP - 261 EP - 268 ER - TY - JOUR A1 - Schuh, Christina A1 - Hercher, David A1 - Stainer, Michaela A1 - Hopf, Rudolf A1 - Teuschl, Andreas A1 - Schmidhammer, Robert A1 - Redl, Heinz T1 - Extracorporeal shockwave treatment: A novel tool to improve Schwann cell isolation and culture JF - Cytotherapy KW - Extracorporeal shockwave Y1 - 2018 ER - TY - GEN A1 - Teuschl, Andreas A1 - Nürnberger, Sylvia A1 - Heimel, Patrick A1 - Redl, Heinz A1 - Nau, Thomas T1 - Regeneration of the Anterior Cruciate Ligament Using a Silk-Fiber Based Scaffold - Histological Results KW - Tissue Regeneration KW - Ligaments KW - Scaffold Y1 - 2018 ER - TY - JOUR A1 - Nau, Thomas A1 - Redl, Heinz A1 - Teuschl, Andreas T1 - Comment on: In Vivo Evaluation of Electrospun Polycaprolactone Graft for Anterior Cruciate Ligament Engineering JF - Tissue Engineering Part A KW - Grafting KW - Ligament Y1 - 2018 ER - TY - JOUR A1 - Gauillaume, Oliver A1 - Teuschl, Andreas A1 - Gruber-Blum, Simone T1 - Emerging Trends in Abdominal Wall Reinforcement: Bringing Bio-Functionality to Meshes JF - Advanced Healthcare Materials KW - Abdominal Wall Reinforcement Y1 - ER - TY - JOUR A1 - Nau, Thomas A1 - Teuschl, Andreas T1 - Regeneration of the anterior cruciate ligament: Current strategies in tissue engineering JF - World Journal of Orthopedics KW - Ligament KW - Regeneration KW - Tissue Engineering Y1 - VL - 6 IS - 1 ER - TY - THES A1 - Weihs, Anna T1 - Elucidation of extracorporeal shock wave treatment triggered intracellular processes N2 - Die Wirkung von Stoßwellen auf den menschlichen Körper ist bereits seit Jahrzehnten bekannt. Ihr zerstörerisches Potential wird seit den 1980-ern zur Behandlung und Desintegration von Nierensteinen angewendet, wo die Stoßwellentherapie heutzutage die Standardbehandlung darstellt. Der erste unerwartete Effekt der Therapie - die Verdichtung des Beckenkammes - wurde schon damals bei Folgeuntersuchungen von Patienten mit Nierensteinen festgestellt. Die Beobachtung, dass Stoßwellen Effekte auf Knochen hervorrufen, ebnete den Weg für die Anwendung der Stoßwellentherapie in Bereichen abseits der Urologie. Mittlerweile wird die Stoßwellentherapie nicht nur zur Behandlung von Knochenbruchheilungsstörungen und Tendinopathien, sondern auch bei Weichteilwundheilungsstörungen eingesetzt. Trotz der vielfältigen Anwendungsgebiete ist der zugrunde liegende Wirkungsmechanismus des positiven Effekts der Stoßwellentherapie bis heute noch nicht vollständig aufgeklärt. Die Aktivierung von mechanotransduktiven Signalwegen wurde bereits teilweise in vitro und in vivo gezeigt, jedoch meist bei knöchernen Indikationen. Doch auch für die Anwendung der Stoßwellentherapie in der (verzögerten) Wundheilung ist die Aufklärung des Wirkungsmechanismus essentiell. Erst dadurch könnte diese nicht- invasive, effiziente und großteils Nebenwirkungs-freie Therapieform auch in diesem Bereich als mögliche Standardtherapieform genutzt werden. In dieser Dissertation wurde zuerst ein Set-up zur in vitro Stoßwellenanwendung optimiert. Mithilfe eines Molekül-Aufnahme Assays wurden die technischen Parameter für die in vitro Stoßwellenbehandlung festgelegt. Mit diesem in vitro Aufbau sollten daraufhin jene intrazellulären Mechanismen identifiziert werden, die von der Stoßwelle beeinflusst werden. In einem in vivo Modell sollte schlussendlich die Rolle dieser Mechanismen am wundheilungsfördernden Effekt der Stoßwelle geklärt werden. Um möglichst universelle Effekte der Stoßwelle auf intrazelluläre Signalwege zu untersuchen, wurden verschiedenste Zelllinien verwendet. Diese beinhalteten die humane U937 Monozyten Zelllinie, humane Jurkat T-Zellen, die humane MG63 Osteosarcoma Zelllinie, die murine C3H10T1/2 mesenchymale Progenitor Zelllinie sowie primäre humane mononukleäre Zellen des peripheren Blutes. Die in den proliferativen Effekt der Stowellentherapie involvierte Signalkaskade konnte darauffolgend erstmals sowohl in murinen C3H10T1/2 Zellen als auch in humanen Fettstammzellen (adipose tissue-derived stem cells) und in humanen Jurkat T-Zellen detailliert beschrieben werden. Außerdem wurde ATP als entscheidendes Signalmolekül identifiziert, welches nach Freisetzung durch Stoßwellenbehandlung mittels purinergem Signaling die Erk1/2 Signalkaskade aktiviert. Für die dadurch gesteigerte Proliferation in stoßwellenbehandelten Zellen ist die Erk1/2 Aktivierung essentiell. In einem in vivo Model für gestörte Wundheilung in der Ratte wurde die Hypothese zur entscheidenden Rolle des Erk1/2 Signalweges im wundheilungsfördernden Effekt der Stoßwelle bestätigt. In dieser Dissertation wurde gezeigt, dass die durch purinerges Signaling aktivierte Erk1/2 Signalkaskade eine entscheidende Rolle in der durch Stoßwellentherapie beschleunigten Zellproliferation in vitro und Wundheilung in vivo übernimmt. Das damit erweiterte Verständnis der Wirkungsmechanismen der Stoßwellentherapie kann zur Weiterentwicklung dieser Behandlungsform als zukünftige Standardtherapie bei Wundheilungsstörungen, wie z.B. für diabetische oder chronische Wunden, beitragen. N2 - Since the early 1980s, shock wave treatment has been the golden standard treatment option for the disintegration of kidney stones in urology. A wide range of beneficial effects of shock waves on the human body was soon identified, starting with first observations of bone densification at the iliac crest after treatment of kidney stones. Since then, the indications for shock wave therapy have conquered areas apart from the field of urology. Nowadays, shock wave therapy is used for a variety of indications such as tendinopathies or impaired bone healing. Furthermore, patients suffering from poor wound healing such as diabetic foot ulcers and also chronic, non-healing wounds are treated successfully with shock waves. Despite the versatile application fields of shock wave therapy, the general principles underlying the beneficial effect of this treatment still remain to be fully elucidated. Several in vitro and in vivo studies, mostly involving osteoblast like cells and the osteo-inductive potential of shock wave treatment, already highlighted the role of the activation of mechanotransductory signaling pathways. For the clinical application of shock wave therapy as an accepted treatment for critically healing wounds (e.g. chronic or diabetic wounds, burns), general mechanistic evidence to explain the underlying mechanisms is essential. These data would facilitate the standardized application of this non-invasive, cost efficient and low- risk bearing therapy, which can be performed in an outpatient setting. First of all, an in vitro set-up was established and the necessary technical parameters for the optimal application of shock wave treatment on cell cultures were defined in this thesis. For this purpose, a molecule uptake assay was used as a functional assay. The following aims of this study were to elucidate the effect of shock wave treatment on intracellular signaling in vitro and to ultimately describe their role in the wound healing effect of shock wave treatment in vivo. To identify universal effects of shock wave treatment on intracellular signaling mechanisms, various cell lines were used, including the human U937 monocytic cell line, a human Jurkat T-cell line, the human MG63 osteosarcoma cell line, the C3H10T1/2 mouse mesenchymal progenitor cell line as well as primary human peripheral mononuclear cells. For the first time, the affected signaling cascade leading to the proliferative effect of shock wave treatment in vitro was described in detail in mouse C3H10T1/2 cells as well as in human adipose tissue-derived stem cells and human Jurkat T-cells. Further, ATP release from shock wave treated cells was shown to initiate intracellular Erk1/2 signaling activation via purinergic signaling. The thereby ultimately increased proliferation was reported to be dependent on shock wave treatment triggered Erk1/2 pathway activation. An in vivo study on impaired wound healing in rats confirmed the hypothesis on the essential role of Erk1/2 signaling in the shock wave treatment induced wound healing effect. Data clearly indicate the crucial importance of the Erk1/2 signaling cascade in the proliferative and wound healing effect of shock wave treatment. Conclusively, purinergic signaling activated Erk1/2 signaling cascades play an essential role in the shock wave treatment induced proliferative and wound healing effect. The thereby broadened knowledge on the underlying mechanistic principles of the effect of shock wave treatment contributes to the establishment of shock wave therapy as a feasible standard treatment for soft tissue wound healing disorders such as diabetic or chronic wounds. KW - Healing KW - Shockwave Y1 - 2018 ER - TY - CHAP A1 - Tkachenko Bril, Andres Igor A1 - David, Veronika A1 - Scherer, Matthias A1 - Jagos, Harald A1 - Kafka, Patricia A1 - Sabo, Anton T1 - Development of a Wearable Live-feedback System to Support Partial Weight-bearing While Recovering From Lower Extremity Injuries T2 - Proceedings of the 11th Conference of the International Sports Engineering Association (ISEA) 2016 KW - Injuries KW - Rehabilitation Y1 - 2018 ER - TY - GEN A1 - Tkachenko Bril, Andres Igor A1 - David, Veronika A1 - Scherer, Matthias A1 - Jagos, Harald A1 - Kafka, Patricia A1 - Sabo, Anton T1 - Development of a Wearable Live-feedback System to Support Partial Weight-bearing While Recovering From Lower Extremity Injuries KW - Injuries KW - Rehabilitation Y1 - 2018 ER - TY - JOUR A1 - Draper, Nick, A1 - Giles, David A1 - Schöffl, Volker A1 - Watts, Phillip A1 - Wolf, Peter A1 - Balas, Jiri A1 - Espana-Romero, Vanesa A1 - Gonzalez, Gina A1 - Fryer, Simon A1 - Fanchini, Maurizio A1 - Vigouroux, Laurent A1 - Seifert, Ludovic A1 - Donath, Lars A1 - Spoerri, Manuel A1 - Bonetti, Kelios A1 - Phillips, Kevin A1 - Stöcker, Urs A1 - Bourassas-Moreau, Felix A1 - Garrido, Inmaculada A1 - Drum, Scott A1 - Beekmeyer, Stuart A1 - Ziltener, Jean.Luc A1 - Taylor, Nicola A1 - Beeretz, Ina A1 - Mally, Franziska A1 - Amca, Arif Mithat A1 - Linhart, Caroline A1 - Abreu, Edgardo T1 - Comparative grading scales, statistical analysis, climber dscriptors and ability grouping JF - Sports Technology KW - Statistics Y1 - 2018 ER - TY - JOUR A1 - Jagos, Harald A1 - David, Veronika A1 - Haller, Michael A1 - Kotzian, Stefan A1 - Hofmann, Manuela A1 - Schlossarek, Stefan A1 - Eicholzer, Karoline A1 - Winkler, Manfred A1 - Frohner, Matthias A1 - Reichel, Martin A1 - Mayr, Winfried A1 - Rafolt, Dietmar T1 - A Framework for (Tele-) Monitoring of the Rehabilitation Progress in Stroke Patients: eHealth 2015 Special Issue. JF - Applied Clinical Informatics KW - Rehabilitation KW - Stroke Patients KW - eHealth Y1 - 2018 ER - TY - JOUR A1 - Ilhan-Mutlu, Aysegül A1 - Siehs, Christian A1 - Berghoff, Sophie A1 - Ricken, Gerda A1 - Widhalm, Georg A1 - Wagner, Ludwig A1 - Preusser, Matthias T1 - Expression profiling of angiogenesis-related genes in brain metastases of lung cancer and melanoma JF - Tumor Biology KW - Cancer KW - Tumor KW - Genetics Y1 - ER - TY - JOUR A1 - Rosser, Julie A1 - Calvo, Isabel Olmos A1 - Schlager, Magdalena A1 - Purtscher, Michaela A1 - Jenner, Florien A1 - Ertl, Peter T1 - Recent Advances of Biologically Inspired 3D Microfluidic Hydrogel Cell Culture Systems JF - J Cell Biol Cell Metalab KW - Hydrogel KW - Cell Culture Y1 - IS - 2 ER - TY - GEN A1 - Weihs, Anna T1 - Aufklärung des Wirkungsmechanismus der Stoßwellentherapie in der Wundheilung KW - Shockwave Y1 - ER - TY - JOUR A1 - Weihs, Anna A1 - Fuchs, Christiane A1 - Teuschl, Andreas A1 - Hartinger, Joachim A1 - Slezak, Paul A1 - Mittermayr, Rainer A1 - Redl, Heinz A1 - Junger, Wolfgang A1 - Sitte, Harald A1 - Rünzler, Dominik T1 - Shock Wave Treatment Enhances Cell Proliferation and Improves Wound Healing by ATP Release-coupled Extracellular Signal-regulated Kinase (ERK) Activation JF - The Journal of biological chemistry KW - Shockwave Y1 - ER - TY - JOUR A1 - Schuh, Christina A1 - Heher, Philipp A1 - Weihs, Anna A1 - Asmita, Banerjee A1 - Wolbank, Susanne A1 - Mittermayr, Rainer A1 - Redl, Heinz A1 - Rünzler, Dominik A1 - Teuschl, Andreas T1 - Adipose derived stem cells respond to in vitro extracorporeal shockwave treatment with increased stemness and multipotency JF - New Biotechnology KW - Shockwave Y1 - ER - TY - JOUR A1 - Jagos, Harald A1 - David, Veronika A1 - Reichel, Martin A1 - Kotzian, Stefan A1 - Schlossarek, Stefan A1 - Haller, Michael A1 - Rafolt, Dietmar T1 - Tele-monitoring of the rehabilitation progress in stroke patients JF - Studies in Health Technology and Informatics KW - Rehabilitation KW - Stroke Patients Y1 - 2018 ER - TY - GEN A1 - Kroller, Theophil A1 - Kreuzinger, Norbert A1 - Rünzler, Dominik T1 - Validierung und Anwendung eines zellkultur-basierten Assays für Screening von endokrin wirksamen Stoffen in Abwasser KW - Assay KW - Sewage Water Y1 - 2018 ER - TY - JOUR A1 - Jagos, Harald A1 - David, Veronika A1 - Reichel, Martin A1 - Rafolt, Dietmar T1 - ele-monitoring of the rehabilitation progress in stroke patients JF - Studies in health technology and informatics KW - Telemonitoring Y1 - ER - TY - GEN A1 - Guillaume, Olivier A1 - Monforte Vila, Xavier T1 - Development of a Biodegradable Silk Mesh Suitable for Intra-Operative Fast Cell Seeding KW - Silk KW - Cell Seeding Y1 - 2018 ER - TY - GEN A1 - Fuchs, Christiane T1 - Shockwave treatment promotes proliferation via purinergic signaling coupled ERK1/2 pathway activation KW - Shockwave Y1 - ER - TY - GEN A1 - Heher, Philipp A1 - Fuchs, Christiane A1 - Prüller, Johanna A1 - Maleiner, Babette A1 - Kollmitzer, Josef A1 - Rünzler, Dominik A1 - Teuschl, Andreas A1 - Wolbank, Susanne A1 - Redl, Heinz T1 - A bioreactor-based 3D culture system for skeletal muscle engineering in fibrin scaffolds KW - Bioreactors KW - Cell Culture KW - Muscle KW - Scaffold Y1 - 2018 ER - TY - JOUR A1 - Banerjee, Asmita A1 - Nürnberger, Sylvia A1 - Hennerbichler, Simone A1 - Riedl, Stefan A1 - Schuh, Christina A1 - Hacobian, Ara A1 - Teuschl, Andreas A1 - Eibl, Jürgen A1 - Redl, Heinz T1 - In toto differentiation of human amniotic membrane towards the Schwann cell lineage JF - 227-239 KW - Membrane KW - In toto differentiation Y1 - 2018 VL - 15 IS - 2 ER - TY - JOUR A1 - Teuschl, Andreas A1 - Neutsch, Lukas A1 - Monforte Vila, Xavier A1 - Rünzler, Dominik A1 - van Griensven, Martijn A1 - Gabor, Franz A1 - Redl, Heinz T1 - Enhanced cell adhesion on silk fibroin via lectin surface modification. JF - Acta Biomaterialia KW - Silk KW - Fibrin Y1 - ER - TY - GEN A1 - Weihs, Anna T1 - Revealing underlying mechanisms of shock wave treatment induced wound healing KW - Shockwave Y1 - ER - TY - JOUR A1 - Buta, Christiane A1 - David, Robert A1 - Dressel, Ralf A1 - Emgard, Mia A1 - Fuchs, Christiane A1 - Gross, Ulrike A1 - Healy, Lyn A1 - Hescheler, Jürgen A1 - Kolar, Roman A1 - Martin, Ulrich A1 - Mikkers, Harald A1 - Müller, Franz-Josef A1 - Schneider, Rebekka A1 - Seiler, Andrea A1 - Spielmann, Horst A1 - Weitzer, Georg T1 - REconsidering pluripotency tests: do we still need teratoma assays? JF - Stem Cell Research KW - Assay KW - Stem Cells Y1 - 2018 VL - 11 IS - 1 SP - 552 EP - 562 ER - TY - JOUR A1 - Hohlrieder, Manfred A1 - Teuschl, Andreas A1 - Cicha, Klaus A1 - van Griensven, Martijn A1 - Redl, Heinz A1 - Stampfl, Jürgen T1 - Bioreactor and scaffold design for the mechanical stimulation of anterior cruciate ligament grafts JF - Biomedical materials and engineering KW - Bioreactors KW - Ligaments KW - Grafting Y1 - 2018 VL - 23 IS - 3 SP - 225 EP - 237 ER - TY - JOUR A1 - Dungel, Peter A1 - Teuschl, Andreas A1 - Banerjee, Asmita A1 - Paier-Pourani, Jamile A1 - Redl, Heinz A1 - Kozlov, Andrey T1 - Impact of mitochondria on nitrite metabolism in HL-1 cardiomyocytes JF - Frontiers in Physiology KW - Nitrite KW - Metabolism Y1 - 2018 IS - 4 ER - TY - JOUR A1 - Teuschl, Andreas A1 - Nürnberger, Sylvia A1 - Redl, Heinz A1 - Nau, Thomas T1 - Articular cartilage tissue regeneration: current research strategies and outlook for the future JF - European Surgery KW - Tissue Regeneration KW - Cartilage Tissue Y1 - 2018 VL - 45 IS - 3 SP - 142 EP - 153 ER - TY - JOUR A1 - Teuschl, Andreas A1 - van Griensven, Martijn A1 - Redl, Heinz T1 - Sericin removal from raw Bombys mori silk scaffolds of high hierarchical order JF - Tissue Eng Part C Methods KW - Scaffold KW - Sericin Y1 - 2018 ER - TY - JOUR A1 - Schuh, Christina A1 - Banerjee, Asmita A1 - Mosia, Shorena A1 - Hopf, Rudolf A1 - Grasl, Christian A1 - Schima, Heinrich A1 - Schmidhammer, Robert A1 - Redl, Heinz A1 - Rünzler, Dominik A1 - Morton, Tatjana J. T1 - Activated Schwann-like cells guided by fibrin structures enhance Axonal Regeneration JF - JOURNAL OF TISSUE ENGINEERING AND REGENERATIVE MEDICINE KW - Cells KW - Fibrin Y1 - 2019 VL - 2012 IS - vol. 6/no. 1 ER - TY - JOUR A1 - Rieder, Bernhard A1 - Weihs, Anna A1 - Teuschl, Andreas A1 - Knebl, Gerald A1 - Kollmitzer, Josef A1 - Redl, Heinz A1 - Rünzler, Dominik T1 - Evaluation of cell response on permanent and pulsed atmospheric pressure stressed cells JF - Journal of Tissue Engineering and Regenerative Medicine KW - Cells KW - Atmospheric Pressure Y1 - 2018 VL - 1 IS - 6 SP - 240 EP - 240 ER - TY - JOUR A1 - Weihs, Anna A1 - Junger, Wolfgang A1 - Schaden, Wolfgang A1 - Sitte, Harald A1 - Rünzler, Dominik T1 - Extracorporeal shockwave treatment induced extracellular ATP release - a potential mechanism to activate wound healing JF - Journal of Tissue Engineering and Regenerative Medicine KW - Shockwave treatment KW - Healing Processes Y1 - 2018 VL - 1 IS - 6 SP - 381 EP - 381 ER - TY - JOUR A1 - Rünzler, Dominik A1 - Weihs, Anna A1 - Junger, Wolfgang A1 - Sitte, Harald A1 - Schaden, Wolfgang T1 - Extracorporeal shock waves - a novel method of mechanostimulation in tissue regeneration JF - Journal of Tissue Engineering and Regenerative Medicine KW - Shockwave treatment KW - Mechanostimulation Y1 - 2018 VL - 1 IS - 6 SP - 380 EP - 380 ER - TY - JOUR A1 - Martin M., Frank A1 - Kollmitzer, Josef A1 - Redl, Heinz A1 - Rünzler, Dominik T1 - Shear force stimulation of adipose-tissue derived stem cells in a novel bioreactor JF - Journal of Tissue Engineering and Regenerative Medicine KW - Stem Cells KW - Adipose Y1 - 2018 VL - 1 IS - 6 SP - 340 EP - 340 ER - TY - GEN A1 - Weihs, Anna A1 - Junger, Wolgang G. A1 - Schaden, Wolfgang A1 - Sitte, Harald H. A1 - Rünzler, Dominik T1 - Extracellular ATP release - a potential benefit in shock wave treatment KW - Shockwave Y1 - 2019 ER - TY - JOUR A1 - Kratochwill, Klaus A1 - Lechner, Michael A1 - Lichtenauer, Anton Michael A1 - Herzog, Rebecca A1 - Lederhuber, Hans Christian A1 - Siehs, Christian A1 - Endemann, Michaela A1 - Mayr, Bernd A1 - Rizzi, Andreas A1 - Aufricht, Christoph T1 - Interleukin-1 receptor-mediated inflammation impairs the heat shock response of human mesothelial cells JF - American Journal of Pathology KW - Human Cells Y1 - 2019 SP - 1544 EP - 1555 ER - TY - JOUR A1 - Führer, Hans Peter A1 - Blöschl, Ingrid A1 - Siehs, Christian A1 - Hassl, Andreas T1 - Detection of Toxoplasma gondii, Neospora caninum, and Encephalitozoon cuniculi in the brains of common voles (Microtus arvalis) and water voles (Arvicola terrestris) by gene amplification techniques in western Austria (Vorarlberg) JF - Parasitol Res. KW - Voles Y1 - 2019 IS - 107(2) ER - TY - CHAP A1 - Jagos, Harald A1 - Oberzaucher, Johannes A1 - Reichel, Martin A1 - Zagler, Wolfgang A1 - Hlauschek, Walter T1 - A Multimodal Approach for Insole Motion Measurement and Analysis T2 - Proceedings of 8th Conference of the International Sports Engineering Association, Wien KW - Health data KW - Insole Y1 - 2019 ER - TY - JOUR A1 - Milan-Lobo, Laura A1 - Gsandtner, Ingrid A1 - Gaubitzer, Erwin A1 - Rünzler, Dominik A1 - Buchmayer, Florian A1 - Köhler, Gottfried A1 - Bonci, Antonello A1 - Freissmuth, Michael A1 - Sitte, Harald H. T1 - Subtype-specific differences in corticotropin-releasing factor receptor complexes detected by fluorescence spectroscopy JF - Molecular Pharmacology (mol pharmacol) KW - Spectroscopy Y1 - 2019 IS - 76(6) SP - 1196 EP - 1210 ER - TY - CHAP A1 - Weihs, Anna A1 - Knebl, Gerald A1 - Redl, Heinz A1 - Rünzler, Dominik T1 - Evaluation of cell migration methods in 3D hydrogels for tissue engineering applications T2 - 3. Forschungsforum der österreichischen Fachhochschulen / Fachhochschule Kärnten KW - Cells Y1 - 2019 SN - 978-3-853912850 SP - 490 EP - 491 ER - TY - GEN A1 - Weihs, Anna T1 - Establishment of an in vitro experimental set-up to evaluate the biological effects of extracorporeal shock wave treatment KW - Shockwave Y1 - 2019 ER - TY - CHAP A1 - Mandl, Thomas A1 - Martinek, Johannes A1 - Mayr, Winfried A1 - Rattay, Frank A1 - Reichel, Martin T1 - Towards a numerical 3D model of Functional Electrical Stimulation of denervated, degenerated human skeletal muscle T2 - Proceedings of the 21st European Modeling and Simulation Symposium KW - Electrical Stimulation KW - Muscle Stimulation KW - Skeletal Muscle Y1 - 2019 ER - TY - CHAP A1 - Martinek, Johannes A1 - Mandl, Thomas A1 - Mayr, Winfried A1 - Rattay, Frank A1 - Reichel, Martin T1 - Coupling Effects in the Bidomain Model of Functional Electrical Stimulation T2 - Proceedings of the 21st European Modeling and Simulation Symposium KW - Electrical Stimulation Y1 - 2019 ER - TY - JOUR A1 - Martinek, Johannes A1 - Stickler, Yvonne A1 - Reichel, Martin A1 - Mayr, Winfried A1 - Rattay, Frank T1 - A novel approach to simulate Hodgkin-Huxley-like excitation with COMSOL multiphysics JF - Artificial Organs KW - Multiphysics Y1 - 2019 VL - 32 IS - 8 SP - 614 EP - 619 ER - TY - GEN A1 - Martinek, Johannes A1 - Stickler, Yvonne A1 - Mayr, Winfried A1 - Rattay, Frank T1 - Influence of the electrode position on the electrical field distribution and excitation of a denervated muscle fiber KW - Muscle Fiber KW - Muscle Excitation Y1 - 2019 ER - TY - CHAP A1 - Knebl, Gerald A1 - Weihs, Anna A1 - Weingant, Michaela A1 - Steininger, Thomas A1 - Redl, Heinz A1 - Rünzler, Dominik T1 - Automatisierte Datenauswertung eines Boyden-Mikro-Chemotaxis-Kammer Zell-Migrations Assays. T2 - 2. Forschungsforum der österreichischen Fachhochschulen KW - Cells KW - Data Analysis Y1 - 2019 SN - 978-3-8322-7023-0 SP - 412 EP - 417 ER - TY - CHAP A1 - Rünzler, Dominik A1 - Knebl, Gerald A1 - Peterbauer, A. A1 - Wolbank, Susanne A1 - Morton, Tatjana J. A1 - Redl, Heinz T1 - Darstellung fluoreszenzmarkierter adulter Stammzellen in drei-dimensionaler Zellkultur mittels Konfokaler Laser Scanning Mikroskopie T2 - Erstes Forschungsforum der österreichischen Fachhochschulen KW - Cells Y1 - 2019 SP - 311 EP - 316 ER - TY - CHAP A1 - Frohner, Matthias A1 - Urbauer, Philipp A1 - Bauer, Martin A1 - Schmidt, Johannes A1 - Escorihuela Navarro, Ana A1 - Siehs, Christian T1 - Applying standardized communication on personal health and sports devices T2 - Tagungsband der eHealth und eHealth Benchmarking KW - Healthcare KW - Sport KW - Standards Y1 - 2019 ER - TY - JOUR A1 - Stickler, Yvonne A1 - Martinek, Johannes A1 - Hofer, Christian A1 - Rattay, Frank T1 - A finite element model of the electrically stimulated human thigh: changes due to denervation and training JF - Artifical Organs KW - Electrical Stimulation KW - Muscle Stimulation Y1 - 2019 IS - Volume 32 Issue 8 SP - 620 EP - 624 ER - TY - CHAP A1 - Reichel, Martin A1 - Martinek, Johannes A1 - Koppelstätter, Norbert T1 - Angewandte Informatik-Jahresprojekte in selbstorganisierten Gruppen, gewichtete Leistungsbeurteilung T2 - DICFO-Beispiele guter Lehre an Fachhochschulen KW - Didactics KW - Teaching KW - Computer Science Y1 - 2019 SP - 45 EP - 48 ER - TY - GEN A1 - Frohner, Matthias A1 - Urbauer, Philipp A1 - Schmidt, Johannes A1 - Escorihuela Navarro, Ana T1 - Applying standardized communication on personal health and sports devices KW - Healthcare KW - Sport KW - Standards Y1 - 2019 ER - TY - CHAP A1 - Reichel, Martin A1 - Illes, Sandrina A1 - Hren, Martin A1 - Reichel, Martin A1 - Sabo, Anton T1 - Influence of Pedal Foot Position on Muscular Activity during Ergometer Cycling (P39) T2 - The Engineering of Sport 7 KW - Sport KW - Cycling Y1 - 2019 SP - 215 EP - 222 ER - TY - CHAP A1 - Martinek, Johannes A1 - Stickler, Yvonne A1 - Reichel, Martin A1 - Rattay, Frank T1 - Simulating Hodgkin-Huxley like Excitation using Comsol Multiphysics T2 - Proceeding Comsol Conference, Hannover (Germany) KW - Multiphysics Y1 - 2019 ER - TY - JOUR A1 - Grunt, Thomas A1 - Tomek, Katharina A1 - Wagner, Renate A1 - Puckmair, Klaudia A1 - Kainz, Birgit A1 - Rünzler, Dominik A1 - Gaiger, Alexander A1 - Köhler, Gottfried A1 - Zielinski, Christoph T1 - Upregulation of retinoic acid receptor-beta by the epidermal growth factor-receptor inhibitor PD153035 is not mediated by blockade of ErbB pathways JF - Journal of Cell Physiology KW - Cells Y1 - 2019 IS - 211(3) SP - 803 EP - 815 ER - TY - JOUR A1 - Spurny, Radovan A1 - Abdoulrahman, Kamaran A1 - Janda, Lubomir A1 - Rünzler, Dominik A1 - Köhler, Gottfried A1 - Castanon, Maria J. A1 - Wiche, Gerhard T1 - Oxidation and nitrosylation of cysteines proximal to the intermediate filament (IF)-binding site of plectin: effects on structure and vimentin binding and involvement in IF collapse JF - Journal of Biological Chemistry KW - Oxidation Y1 - 2019 IS - 282(11) SP - 8175 EP - 8187 ER - TY - JOUR A1 - Meyerspeer, Martin A1 - Mandl, Thomas A1 - Reichel, Martin A1 - Mayr, Winfried A1 - Hofer, Christian A1 - Kern, Helmut A1 - Moser, Ewald T1 - Effects of functional electrical stimulation in denervated thigh muscles of paraplegic patients mapped with T2 imaging JF - MAGMA KW - Electrical Stimulation KW - Muscle Stimulation Y1 - 2019 VL - 21 IS - 3 SP - 219 EP - 226 ER - TY - GEN A1 - Rünzler, Dominik T1 - Darstellung fluoreszenzmarkierter adulter Stammzellen in drei-dimensionaler Zellkultur mittels Konfokaler Laser Scanning Mikroskopie KW - Cells Y1 - 2019 ER - TY - GEN A1 - Rünzler, Dominik T1 - Automatisierte Datenauswertung eines Boyden-Mikro-Chemotaxis-Kammer Zell-Migrations Assays KW - Cells KW - Data Analysis Y1 - 2019 ER - TY - GEN A1 - Sabo, Anton A1 - Eckelt, Markus A1 - Reichel, Martin T1 - Sicherheit des Sportgerätes Kite KW - Sport KW - Security Y1 - 2019 ER - TY - CHAP A1 - Milan-Lobo, Laura A1 - Rünzler, Dominik A1 - Gaubitzer, Erwin A1 - Köhler, Gottfried A1 - Bonci, Antonello A1 - Freissmuth, Michael A1 - Sitte, Harald H. T1 - CRF receptors form dynamic complexes at the plasma membrane but only type 2 receptor increases lateral mobility after CRF binding T2 - FEBS Journal KW - Plasma Membrane Y1 - 2019 ER - TY - CHAP A1 - Kaisler, Raphaela A1 - Pázmándi, Christian A1 - Ortner, Anna A1 - Haberl, Ines A1 - Köhler, Gottfried A1 - Rünzler, Dominik A1 - Artl, Andreas A1 - Andreae, Fritz A1 - Mosgöller, Wilhelm T1 - Vasoactive intestinal peptide conjugate internalisation as strategy for cytoplasmic drug delivery T2 - Journal of Molecular Neuroscience KW - Cytplasmic Drug Delivery Y1 - 2019 ER - TY - JOUR A1 - Wruss, Jürgen A1 - Rünzler, Dominik A1 - Steiger, Christina A1 - Chiba, Peter A1 - Köhler, Gottfried A1 - Blaas, Dieter T1 - Attachment of VLDL receptors to an icosahedral virus along the 5-fold symmetry axis: multiple binding modes evidenced by fluorescence correlation spectroscopy JF - Biochemistry KW - Cells Y1 - 2019 IS - 46(21) SP - 6331 EP - 6339 ER - TY - CHAP A1 - Reichel, Martin A1 - Martinek, Johannes A1 - Mandl, Thomas A1 - Mayr, Winfried A1 - Rattay, Frank T1 - Development of modeling functional electrical stimulation in denervated muscle T2 - Proceedings of the 9th Vienna international workshop on functional electrostimulation KW - Electrical Stimulation KW - Muscle Stimulation KW - Muscle Denervation Y1 - 2019 SP - 159 EP - 162 ER - TY - CHAP A1 - Martinek, Johannes A1 - Stickler, Yvonne A1 - Dohnal, Fahdi A1 - Reichel, Martin A1 - Mayr, Winfried A1 - Rattay, Frank T1 - Simulation der elektrischen Stimulation im menschlichen Oberschenkel mit Hilfe von FEMLAB T2 - Neue Wege der Multiphysik Simulation KW - Muscle Stimulation Y1 - 2019 ER - TY - CHAP A1 - Martinek, Johannes A1 - Stickler, Yvonne A1 - Reichel, Martin A1 - Rattay, Frank T1 - A new approach to simulate Hodgkin - Huxley like excitatation with FEMLAB T2 - Proceedings of the 9-th Vienna international workshop on functional electrostimulation, Krems (Austria) KW - Neurons Y1 - 2019 SP - 163 EP - 166 ER - TY - GEN A1 - Rattay, Frank A1 - Martinek, Johannes A1 - Stickler, Yvonne A1 - Minassian, Karen T1 - Simulationen zur Funktionellen Elektrostimulation: Effektive Elektrodenkonfigurationen zur Muskelstimulation KW - Electrical Stimulation KW - Muscle Stimulation Y1 - 2019 ER - TY - CHAP A1 - Sabo, Anton A1 - Balt, M. A1 - Schrammel, G. A1 - Reichel, Martin T1 - Motion analysis supported by data acquisition during golf swing T2 - The Impact of Technology on Sport II KW - Sport KW - Golf Y1 - 2019 SP - 265 EP - 270 ER - TY - GEN A1 - Sabo, Anton A1 - Eckelt, Markus A1 - Bochdansky, Thomas T1 - Sport als Wirbelsäulenprävention KW - Sport KW - Prevention Y1 - 2019 ER - TY - JOUR A1 - Maier, Christina A1 - Rünzler, Dominik A1 - Schindelar, Julia A1 - Grabner, Gottfried A1 - Waldhäusl, Werner Klaus A1 - Köhler, Gottfried A1 - Luger, Anton T1 - G-protein-coupled glucocorticoid receptors on the pituitary cell membrane JF - Journal of Cell Science KW - Cells Y1 - 2019 IS - 118(15) SP - 3353 EP - 3361 ER - TY - GEN A1 - Sabo, Anton A1 - Bochdansky, Thomas A1 - Kristen, Karl-Heinz T1 - Krafttraining im Vergleich zu Koordinations-Balancetraining der Wirbelsäulenmuskulatur; praxisorientierte Anwendungen im Sport KW - Sport KW - Prevention Y1 - 2019 ER - TY - JOUR A1 - Martinek, Johannes A1 - Reichel, Martin A1 - Rattay, Frank A1 - Mayr, Winfried T1 - Analysis of calculated electrical activation of denervated muscle fibres in the human thigh JF - Artificial Organs KW - Muscle Stimulation KW - Electrical Stimulation KW - Muscle Denervation Y1 - 2019 VL - 29 IS - 6 SP - 444 EP - 447 ER - TY - GEN A1 - Martinek, Johannes A1 - Sticker, Yvonne A1 - Reichel, Martin A1 - Rattay, Frank T1 - Electrical stimulation of denervated skeletal muscles: a modeling study of transcutaneous stimulation KW - Electrical Stimulation KW - Muscle Stimulation Y1 - 2019 ER - TY - GEN A1 - Martinek, Johannes A1 - Reichl, Martin A1 - Strummer, Martin A1 - Schmöllebeck, Fritz T1 - Fachhochschule Technikum Wien – Femlab in der Lehre KW - Laboratory KW - Teaching Y1 - 2019 ER - TY - BOOK A1 - Witte, Kerstin A1 - Edelmann-Nusser, Jürgen A1 - Sabo, Anton A1 - Eckehard, Moritz T1 - Sporttechnologie zwischen Theorie und Praxis IV KW - Sport Y1 - 2019 PB - Shaker Verlag CY - Aachen ER - TY - GEN A1 - Sabo, Anton A1 - Eckelt, Markus A1 - Herczeg, C. T1 - Wechselwirkung zwischen Stocklänge und Nordic Walking Technik KW - Sport KW - Nordic Walking Y1 - 2019 ER - TY - GEN A1 - Sabo, Anton A1 - Bochdansky, Thomas A1 - Kristen, Karl-Heinz T1 - Allgemeine Biomechanik, Physik-Messtechnik im Leistungssport, Orthopädie, Prävention, Rehabilitation und Fitness KW - Sport KW - Biomechanics KW - Prevention KW - Rehabitation Y1 - 2019 ER - TY - GEN A1 - Senger, L. A1 - Pelz, A. A1 - Woller, P. A1 - Reichel, Martin A1 - Sabo, Anton T1 - Game-set-match- eine messtechnisch unterstützte Schlaganalyse KW - Sport Y1 - 2019 ER - TY - GEN A1 - Schrammel, G. A1 - Hacker, M. A1 - Manzl, G. A1 - Zaiser, C. A1 - Reichel, Martin A1 - Sabo, Anton T1 - Golf - the 19th hole KW - Sport KW - Golf Y1 - 2019 ER - TY - GEN A1 - Grafl, D. A1 - Schachner, O. A1 - Stiedl, M. A1 - Stolz, G. A1 - Reichel, Martin A1 - Sabo, Anton T1 - Fischer Skischuh KW - Sport KW - Skiing Y1 - 2019 ER - TY - GEN A1 - Fischer, G. A1 - Herczeg, C. A1 - Reichel, Martin A1 - Sabo, Anton T1 - Der Nordic Walking Schuh KW - Sport KW - Nordic Walking Y1 - 2019 ER - TY - GEN A1 - Magerböck, R. A1 - Ruzicka, G. A1 - Reichel, Martin A1 - Sabo, Anton T1 - Sportgeräteprüftisch KW - Sport Y1 - 2019 ER - TY - GEN A1 - Sabo, Anton A1 - Reichel, Martin T1 - Koordination, was ist das KW - Sport KW - Coordination Y1 - 2019 ER - TY - CHAP A1 - Rattay, Frank A1 - Reichel, Martin A1 - Martinek, Johannes A1 - Persy, Ilse A1 - Resatz, Susanne A1 - Mayr, Winfried T1 - Functional electrical stimulation of denervated skeletal muscles: a modeling study T2 - Proceedings of the 25th Annual International Conference of the Engineering in Medicine and Biology Society KW - Electrical Stimulation KW - Muscle Stimulation KW - Muscle Denervation Y1 - 2019 SP - 1557 EP - 1560 ER - TY - CHAP A1 - Reichel, Martin A1 - Martinek, Johannes A1 - Mayr, Winfried A1 - Rattay, Frank T1 - Functional electrical stimulation of denervated skeletal muscle fibers in 3D human thigh-modeling and simulation T2 - Proceedings of the 8th Vienna international workshop on functional electrostimulation KW - Muscle Stimulation KW - Muscle Denervation KW - Electrical Stimulation Y1 - 2019 SP - 44 EP - 47 ER - TY - JOUR A1 - Reichel, Martin A1 - Sabo, Anton A1 - Kollmitzer, Josef T1 - The effects of different back training systems on differential postural control JF - The Engineering of Sport 5 KW - Sport KW - Back Training Y1 - 2019 IS - Vol. 1 SP - 149 EP - 154 ER - TY - GEN A1 - Sabo, Anton A1 - Reichel, Martin A1 - Bochdansky, Thomas T1 - Posturographie, biomechanische Messtechnik zum Koordinationstest KW - Sport KW - Coordination Y1 - 2019 ER - TY - JOUR A1 - Reichel, Martin A1 - Sabo, Anton T1 - Flex-behaviour of bindings and boots in snowboard-systems JF - The Engineering of Sport 5 KW - Sport KW - Snowboard Y1 - 2019 IS - Vol. 2 SP - 209 EP - 215 ER - TY - GEN A1 - Sabo, Anton A1 - Schrammel, G. A1 - Eckelt, Markus A1 - Bochdansky, Thomas T1 - Messtechnik im Golfsport KW - Sport KW - Golf Y1 - 2019 ER - TY - GEN A1 - Sabo, Anton A1 - Dolezal, M. A1 - Reichel, Martin T1 - Carving ski macht Bandl hin KW - Sport KW - Skiing Y1 - 2019 ER - TY - JOUR A1 - Maier, Christina A1 - Rünzler, Dominik A1 - Wagner, Ludwig A1 - Grabner, Günther A1 - Köhler, Gottfried A1 - Lugner, Anton T1 - Evidence for specific glucocorticoid binding sites on the cell membrane by fluorescence correlation spectroscopy JF - Single Moleculus KW - Cells Y1 - 2019 IS - 3(4) SP - 211 EP - 216 ER - TY - CHAP A1 - Martinek, Johannes A1 - Reichel, Martin A1 - Rattay, Frank A1 - Mayr, Winfried T1 - Simulation and Analysis of Electric Activiation of Denervated Muscle Fibres in the Human Thigh T2 - FEM Workshop / Tagungsbandbeitrag / Ulm (Germany) KW - Electrical Stimulation KW - Muscle Stimulation Y1 - 2019 SP - 103 EP - 107 ER - TY - GEN A1 - Martinek, Johannes A1 - Reichel, Martin A1 - Mayr, Winfried A1 - Rattay, Frank T1 - Analyse der Aktivierung von denervierten Muskelfasern im menschlichen Oberschenkel bei FES KW - Muscle Denervation KW - Thigh KW - Muscle Stimulation Y1 - 2019 ER - TY - GEN A1 - Reichel, Martin A1 - Martinek, Johannes A1 - Rattay, Frank A1 - Mayr, Winfried T1 - Simulation der Funktionellen Elektrostimulation an der denervierten Oberschenkelmuskulatur KW - Electrical Stimulation KW - Muscle Stimulation Y1 - 2019 ER - TY - GEN A1 - Sabo, Anton A1 - Bochdansky, Thomas T1 - Biomechanik sportlicher Bewegungen ausgewählter Sportarten KW - Sport KW - Biomechanics Y1 - 2019 ER - TY - GEN A1 - Sabo, Anton A1 - Kollmitzer, Josef A1 - Bochdansky, Thomas T1 - Krafttraining im Vergleich zu Koordination-Balancetraining KW - Sport Y1 - 2019 ER - TY - CHAP A1 - Sabo, Anton A1 - Houska, M. A1 - Schenner, M. A1 - Osrael, J. T1 - XPRESS - 2000 - Gait and Motion Analysis T2 - XPRESS - 2000 - Gait and Motion Analysis KW - Sport Y1 - 2019 ER - TY - JOUR A1 - Krautwald, Stefan A1 - Büscher, Dirk A1 - Jesenberger, Veronika A1 - Buder, Sylke A1 - Baccarini, Manuela T1 - Involvement of the protein tyrosine phosphatase SHP-1 in Ras-mediated activation of the mitogen-activated protein kinase pathway. JF - Mol Cell Biol. N2 - Ubiquitously expressed SH2-containing tyrosine phosphatases interact physically with tyrosine kinase receptors or their substrates and relay positive mitogenic signals via the activation of the Ras-mitogen-activated protein kinase (MAPK) pathway. Conversely, the structurally related phosphatase SHP-1 is predominantly expressed in hemopoietic cells and becomes tyrosine phosphorylated upon colony-stimulating factor 1 treatment of macrophages without associating with the colony-stimulating factor 1 receptor tyrosine kinase. Mice lacking functional SHP-1 (me/me and me(v)/me(v)) develop systemic autoimmune disease with accumulation of macrophages, suggesting that SHP-1 may be a negative regulator of hemopoietic cell growth. By using macrophages expressing dominant negative Ras and the me(v)/me(v) mouse mutant, we show that SHP-1 is activated in the course of mitogenic signal transduction in a Ras-dependent manner and that its activity is necessary for the Ras-dependent activation of the MAPK pathway but not of the Raf-1 kinase. Consistent with a role for SHP-1 as an intermediate between Ras and the MEK-MAPK pathway, Ras-independent activation of the latter kinases by bacterial lipopolysaccharide occurred normally in me(v)/me(v) cells. Our results sharply accentuate the diversity of signal transduction in mammalian cells, in which the same signaling intermediates can be rearranged to form different pathways. KW - Molecular Cell Biology KW - protein kinase pathway KW - Ras Y1 - 2020 U6 - http://dx.doi.org/doi: 10.1128/mcb.16.11.5955 VL - 1996 IS - 16(11) SP - 5955 EP - 5963 ER - TY - JOUR A1 - Reichel, Martin A1 - Haumer, A. A1 - Schretter, H. A1 - Sabo, Anton T1 - Development of a Measurement-Prosthesis for a Ski Boot Test Bench JF - The Engineering of Sport KW - Sport KW - Skiing Y1 - 2019 VL - Vol. 1 IS - 7 SP - 255 EP - 262 ER - TY - JOUR A1 - Stickler, Yvonne A1 - Martinek, Johannes A1 - Rattay, Frank T1 - Modeling Needle Stimulation of Denervated Muscle Fibers: Voltage-Distance Relations and Fiber Polarization Effects JF - IEEE Transactions on Biomedical Engineering / IEEE Engineering in Medicine and Biology Society KW - Muscle Stimulation KW - Electric Stimulation Y1 - 2019 VL - Vol. 56 IS - issue 10 ER - TY - JOUR A1 - Stickler, Yvonne A1 - Martinek, Johannes A1 - Reichel, Martin A1 - Rattay, Frank T1 - Modeling the excitability of the denervated human thigh for the stimulation with surface and needle electrodes JF - Basic Applied Myology KW - Electric Stimulation KW - Muscle Stimulation Y1 - 2019 VL - Vol. 19 IS - Issue 5 SP - 237 EP - 242 ER - TY - JOUR A1 - Jesenberger, Veronika A1 - Procyk, Katarzyna A1 - Yuan, Junying A1 - Reipert, Siegfried A1 - Baccarini, Manuela T1 - Salmonella-induced caspase-2 activation in macrophages: a novel mechanism in pathogen-mediated apoptosis JF - J Exp Med. N2 - The enterobacterial pathogen Salmonella induces phagocyte apoptosis in vitro and in vivo. These bacteria use a specialized type III secretion system to export a virulence factor, SipB, which directly activates the host's apoptotic machinery by targeting caspase-1. Caspase-1 is not involved in most apoptotic processes but plays a major role in cytokine maturation. We show that caspase-1-deficient macrophages undergo apoptosis within 4-6 h of infection with invasive bacteria. This process requires SipB, implying that this protein can initiate the apoptotic machinery by regulating components distinct from caspase-1. Invasive Salmonella typhimurium targets caspase-2 simultaneously with, but independently of, caspase-1. Besides caspase-2, the caspase-1-independent pathway involves the activation of caspase-3, -6, and -8 and the release of cytochrome c from mitochondria, none of which occurs during caspase-1-dependent apoptosis. By using caspase-2 knockout macrophages and chemical inhibition, we establish a role for caspase-2 in both caspase-1-dependent and -independent apoptosis. Particularly, activation of caspase-1 during fast Salmonella-induced apoptosis partially relies on caspase-2. The ability of Salmonella to induce caspase-1-independent macrophage apoptosis may play a role in situations in which activation of this protease is either prevented or uncoupled from the induction of apoptosis. KW - caspase-2 activation in macrophages KW - pathogen-activated apoptosis KW - Molecular Cell Biology Y1 - 2020 U6 - http://dx.doi.org/10.1084/jem.192.7.1035 VL - 2000 IS - 192(7) SP - 1035 EP - 1046 ER - TY - JOUR A1 - Jesenberger, Veronika A1 - Procyk, Katarzyna A1 - Rüth, Jochen A1 - Schreiber, Martin A1 - Theussl, Hans Christian A1 - Wagner, Erwin A1 - Baccarini, Manuela T1 - Protective Role of Raf-1 in Salmonella-Induced Macrophage Apoptosis JF - J Exp Med. N2 - Invasive Salmonella induces macrophage apoptosis via the activation of caspase-1 by the bacterial protein SipB. Here we show that infection of macrophages with Salmonella causes the activation and degradation of Raf-1, an important intermediate in macrophage proliferation and activation. Raf-1 degradation is SipB- and caspase-1–dependent, and is prevented by proteasome inhibitors. To study the functional significance of Raf-1 in this process, the c-raf-1 gene was inactivated by Cre-loxP–mediated recombination in vivo. Macrophages lacking c-raf-1 are hypersensitive towards pathogen-induced apoptosis. Surprisingly, activation of the antiapoptotic mitogen-activated protein kinase kinase (MEK)/extracellular signal–regulated kinase (ERK) and nuclear factor κB pathways is normal in Raf-1–deficient macrophages, and mitochondrial fragility is not increased. Instead, pathogen-mediated activation of caspase-1 is enhanced selectively, implying that Raf-1 antagonizes stimulus-induced caspase-1 activation and apoptosis. KW - Salmonella-Induced Macrophage Apoptosis KW - Raf-1 pathway KW - Molecular Cell Biology Y1 - 2020 U6 - http://dx.doi.org/10.1084/jem.193.3.353 VL - 2001 IS - 193(3) SP - 353 EP - 364 ER - TY - JOUR A1 - Jesenberger, Veronika A1 - Jentsch, Stefan T1 - Deadly encounter: ubiquitin meets apoptosis JF - Nat Rev Mol Cell Biol. N2 - The ubiquitin/proteasome pathway is the main non-lysosomal route for intracellular protein degradation in eukaryotes. It is instrumental to various cellular processes, such as cell-cycle progression, transcription and antigen processing. Recent findings also substantiate a pivotal role of the ubiquitin/proteasome pathway in the regulation of apoptosis. Regulatory molecules that are involved in programmed cell death have been identified as substrates of the proteasome. Moreover, key regulators of apoptosis themselves seem to have an active part in the proteolytic inactivation of death executors. KW - programmed cell death KW - Molecular Cell Biology Y1 - 2020 U6 - http://dx.doi.org/https://doi.org/10.1038/nrm731 VL - 2002 IS - 3(2) SP - 112 EP - 121 ER - TY - CHAP A1 - Mayr, Winfried A1 - Hofer, Christian A1 - Rafolt, Dietmar A1 - Bijak, Manfred A1 - Lanmüller, Hermann A1 - Reichel, Martin A1 - Sauermann, Stefan A1 - Unger, Ewald T1 - The European Project RISE: General Overview and Engineering Aspects T2 - Proceedings of the 8th Vienna International Workshop on FES KW - Engineering Y1 - 2019 ER - TY - CHAP A1 - Litzenberger, Stefan T1 - A Study of Skin-Close Heat and Moisture with Different Types of Backpacks in Cycling. T2 - ISEA 2020 Online - Proceedings 2020, 49(1) KW - Sports Equipment Technologies KW - Cycling Y1 - 2020 SP - 86 EP - 86 ER - TY - JOUR A1 - Sauermann, Stefan ED - RDA-COVID19-Epidemiology WG., Research Data Alliance T1 - Preservation of Individuals’ Privacy in Shared COVID-19 Related Data JF - COVID-19 Data sharing in epidemiology, version 0.054. N2 - This paper provides insight into how restricted data can be incorporated in an open-be-default-by-design digital infrastructure for scientific data. We focus, in particular, on the ethical component of FAIRER (Findable, Accessible, Interoperable, Ethical, and Reproducible) data, and the pseudo-anonymization and anonymization of COVID-19 datasets to protect personally identifiable information (PII). First we consider the need for the customisation of the existing privacy preservation techniques in the context of rapid production, integration, sharing and analysis of COVID-19 data. Second, the methods for the pseudo-anonymization of direct identification variables are discussed. We also discuss different pseudo-IDs of the same person for multi-domain and multi-organization. Essentially, pseudo-anonymization and its encrypted domain specific IDs are used to successfully match data later, if required and permitted, as well as to restore the true ID (and authenticity) in individual cases of a patient's clarification.Third, we discuss application of statistical disclosure control (SDC) techniques to COVID-19 disease data. To assess and limit the risk of re-identification of individual persons in COVID-19 datasets (that are often enriched with other covariates like age, gender, nationality, etc.) to acceptable levels, the risk of successful re-identification by a combination of attribute values must be assessed and controlled. This is done using statistical disclosure control for anonymization of data. Lastly, we discuss the limitations of the proposed techniques and provide general guidelines on using disclosure risks to decide on appropriate modes for data sharing to preserve the privacy of the individuals in the datasets. KW - COVID19 KW - pseudo-anonymization KW - statistical disclosure control KW - data sharing KW - privacy Y1 - 2020 ER - TY - GEN A1 - Leitner, Rita A1 - Sauermann, Stefan T1 - Erreichen „harmonisierter Empfehlungen“: von Studierenden generierte Fragen als Ausgangspunkt für evidenzbasierte Argumentation von Stakeholder-Sichtweisen – ein problem-based learning Ansatz KW - Problem Based Learning KW - Didactics KW - eHealth KW - Education Y1 - ER - TY - CHAP A1 - Litzenberger, Stefan A1 - Christensen, Troels A1 - Hofstätter, Otto A1 - Sabo, Anton T1 - Prediction of Road Surface Quality during Cycling Using Smartphone Accelerometer Data T2 - 12th Conference of the International Sports Engineering Association KW - Cycling KW - Road Surface KW - Machine Learning KW - Accelerometer Data Y1 - ER - TY - CHAP A1 - Gerbovics, Ferenc A1 - Haller, Michael A1 - Bittermann, Mark A1 - Urbauer, Philipp A1 - Frohner, Matthias A1 - Pohn, Birgit A1 - Sauermann, Stefan A1 - Mense, Alexander T1 - ADVANCEMENT OF A WEARABLE HEALTH DATA HUB-PROTOTYPE FOR CONTEXT BASED TELEMONITORING USING STANDARDIZED DATA TRANSFER METHODS T2 - Tagungsband der eHealth und eHealth Benchmarking, Wien KW - Health data KW - Telemonitoring Y1 - 2019 ER - TY - CHAP A1 - Sauermann, Stefan A1 - Reichel, Martin A1 - Bijak, Manfred A1 - Unger, Ewald A1 - Rafolt, D. A1 - Mayr, Winfried A1 - Lanmüller, Hermann T1 - BioSignalizer: Data acquisition and analysis for experimental and clinical applications T2 - IFMBE Proceedings / Vienna, Austria, Verein zur Veranstaltung der EMBEC KW - Data Analysis Y1 - 2019 SP - 408 EP - 409 ER - TY - JOUR A1 - Illes, Sandrina A1 - Hassmann, Michaela A1 - Hren, Martin A1 - Ilagan, Reynaldo A1 - Litzenberger, Stefan A1 - Sabo, Anton T1 - Influence of pedalling with the metatarsus on running characteristics in triathlon competition JF - Procedia Engineering KW - Sport KW - Running Y1 - 2019 VL - 2010 IS - Vol. 2 (2) SP - 3127 EP - 3131 ER - TY - CHAP A1 - Sabo, Anton A1 - Kafka, Patricia A1 - Litzenberger, Stefan A1 - Sabo, Christoph T1 - The Engineering of Sport 8 - Engineering Emotion T2 - Procedia Engineering KW - Sport Y1 - 2019 ER - TY - JOUR A1 - Huber-Gries, Carina A1 - Ilk, Nicola A1 - Rünzler, Dominik A1 - Egelseer, Eva-Maria A1 - Weigert, Stefan A1 - Sleytr, Uwe T1 - The three S-layer-like homology motifs of the S-layer protein SbpA of Bacillus sphaericus CCM 2177 are not sufficient for binding to the pyruvylated secondary cell wall polymer JF - Molecular Microbiology KW - Proteins Y1 - 2018 VL - 55 IS - 1 SP - 197 EP - 205 ER - TY - GEN A1 - Thiele, Gerrit A1 - Kafka, Patricia A1 - Litzenberger, Stefan A1 - Sabo, Anton T1 - Ontrack measurements in motocross: The correlation of neck muscle activity and contact incidents of helmet and neck brace KW - Motocross KW - Neck Y1 - 2018 ER - TY - JOUR A1 - Tomasch, Janine A1 - Maleiner, Babette A1 - Hromada, Carina A1 - Szwarc-Hofbauer, Dorota A1 - Teuschl-Woller, Andreas T1 - Cyclic Tensile Stress Induces Skeletal Muscle Hypertrophy and Myonuclear Accretion in a 3D Model JF - Tissue Eng. Part A. N2 - Skeletal muscle is highly adaptive to mechanical stress due to its resident stem cells and the pronounced level of myotube plasticity. Herein, we study the adaptation to mechanical stress and its underlying molecular mechanisms in a tissue-engineered skeletal muscle model. We subjected differentiated 3D skeletal muscle-like constructs to cyclic tensile stress using a custom-made bioreactor system, which resulted in immediate activation of stress-related signal transducers (Erk1/2, p38). Cell cycle re-entry, increased proliferation, and onset of myogenesis indicated subsequent myoblast activation. Furthermore, elevated focal adhesion kinase and β-catenin activity in mechanically stressed constructs suggested increased cell adhesion and migration. After 3 days of mechanical stress, gene expression of the fusogenic markers MyoMaker and MyoMixer, myotube diameter, myonuclear accretion, as well as S6 activation, were significantly increased. Our results highlight that we established a promising tool to study sustained adaptation to mechanical stress in healthy, hypertrophic, or regenerating skeletal muscle. KW - fibrin KW - tissue engineering KW - tensile stress KW - regeneration KW - hypertrophy Y1 - VL - 2023 IS - Mar SP - 257 EP - 268 ER - TY - GEN A1 - Stoiber, Stefan A1 - Purtscher, Michaela A1 - Gepp, Barbara A1 - Huber-Gries, Carina T1 - Towards a platform for spatially defined cell characterization of a miniturized heart tissue model KW - Microfluidic KW - Tissue Engineering KW - Heart Y1 - ER - TY - GEN A1 - Sauermann, Stefan T1 - Healthcare Records in Austria and the EU Standards for Interoperability KW - Healthcare KW - Interoperability Y1 - ER - TY - GEN A1 - Purtscher, Michaela A1 - Ergir, Ece A1 - Szwarc, Dorota A1 - Monforte Vila, Xavier A1 - Rünzler, Dominik A1 - Huber-Gries, Carina T1 - A microfluidic-based easy-to-use cardiac tissue model for drug screening applications KW - Tissue Generation KW - Drug Screening KW - Cardiac Y1 - 2018 ER - TY - GEN A1 - Purtscher, Michaela A1 - Ergir, Ece A1 - Monforte Vila, Xavier A1 - Huber-Gries, Carina T1 - Establishment of an in vitro heart tissue model for pre-clinical screening of therapeutic agents using microfluidic technology KW - Tissue Generation KW - Heart Tissue Y1 - 2018 ER - TY - GEN A1 - Purtscher, Michaela A1 - Ergir, Ece A1 - Szwarc, Dorota A1 - Huber-Gries, Carina T1 - Microfluidic based heart-tissue model for directed development of cardiac specific cell types KW - Heart Tissue KW - Tissue Generation KW - Cardiac Y1 - 2018 ER - TY - CHAP A1 - Russold, Michael A1 - Bijak, Manfred A1 - Sauermann, Stefan A1 - Reichel, Martin A1 - Rakos, Monika A1 - Mayr, Winfried T1 - Object oriented Software Components for Multichannel-Stimulator Control T2 - 7th Vienna International Workshop on FES / Department of Biomedical Engineering and Physics, University of Vienna KW - Stimulator Y1 - 2019 SP - 254 EP - 257 ER - TY - JOUR A1 - Behrmann, Lasse A1 - Litzenberger, Stefan A1 - Mally, Franziska A1 - Sabo, Anton T1 - Evaluation of Bending and Torsional Properties of Different Ice Hockey Sticks JF - Procedia Engineering KW - Ice Hockey Y1 - ER - TY - GEN A1 - Sauermann, Stefan A1 - David, Veronika A1 - Schlögl, Alois A1 - Egelkraut, Reinhard A1 - Frohner, Matthias A1 - Pohn, Birgit A1 - Urbauer, Philipp A1 - Mense, Alexander T1 - Biosignals, Standards and FHIR – The Way to Go? KW - Biosignals KW - IT Standards Y1 - 2018 ER - TY - THES A1 - Frohner, Matthias T1 - Quality Criteria and Concepts for the Development of Mobile Health Applications KW - mHealth KW - Quality KW - Telemonitoring KW - Communication Standards KW - Validation Y1 - ER - TY - JOUR A1 - Ruess, Christian A1 - Kristen, Karl-Heinz A1 - Eckelt, Markus A1 - Mally, Franziska A1 - Litzenberger, Stefan A1 - Sabo, Anton T1 - Stand up Paddle Surfing - An Aerobic Workout and Balance Training JF - Procedia Engineering KW - Stand-Up Paddling Y1 - 2018 VL - 60 IS - 0 SP - 62 EP - 66 ER - TY - JOUR A1 - Schindelka, Bernhard A1 - Litzenberger, Stefan A1 - Sabo, Anton T1 - Body Climate Differences for Men and Women Wearing Functional Underwear during Sport at Temperatures Below Zero Degrees Celsius JF - Procedia Engineering KW - Body Climate KW - Underwear Y1 - 2018 VL - 60 IS - 0 SP - 46 EP - 50 ER - TY - JOUR A1 - Mally, Franziska A1 - Litzenberger, Stefan A1 - Sabo, Anton T1 - Surface Electromyography Measurements of Dorsal Muscle Cross-Activation in Therapeutic Climbing JF - Procedia Engineering KW - Muscle Activity KW - Climbing Y1 - 2018 VL - 60 IS - 0 SP - 22 EP - 27 ER - TY - CHAP A1 - Litzenberger, Stefan T1 - The Influence of Backpack-Design on Thermophysiological Parameters during Simulated Hiking Activities. T2 - ISEA 2020 Online - Proceedings 2020, 49(1) KW - Sports Equipment Technologies KW - Hiking Y1 - 2020 SP - 59 EP - 59 ER - TY - JOUR A1 - Sauermann, Stefan T1 - COVID-19 Questionnaires, Surveys, and Item-Banks: Overview of Clinical- and Population-Based Instruments JF - SSRN N2 - New COVID-19 related instruments are being rapidly developed around the world to collect patient- and population-based information. Heterogeneity between instruments limits comparability of results. The present study provides an overview of instruments and resources. We scoped the content domain on a selection of instruments using the Maelstrom taxonomy. Content of the instruments varied widely, from proximal measures (e.g., clinical symptoms, comorbidities, etc.) to distal measures such as political attitudes. We recommend that researchers reuse existing instruments to the greatest extent possible, and that they make results openly available in machine-readable format to facilitate reuse and maximize comparability of results across studies and countries. KW - COVID19 KW - Questionnaires KW - SARS-COV2 Y1 - 2020 ER - TY - JOUR A1 - Ruess, Christian A1 - Kristen, Karl-Heinz A1 - Eckelt, Markus A1 - Mally, Franziska A1 - Litzenberger, Stefan A1 - Sabo, Anton T1 - Acticvity of Trunk and Leg Muscles during Stand Up Paddle Surfing JF - Procedia Engineering KW - Muscle Activity KW - Stand-Up Paddling Y1 - 2018 VL - 60 IS - 0 SP - 57 EP - 61 ER - TY - GEN A1 - Sauermann, Stefan A1 - Schwarz, René T1 - Normenbaukasten für IT-Interoperabilität als Tool für das Risikomanagement KW - Interoperability Y1 - ER - TY - GEN A1 - Sauermann, Stefan A1 - Frohner, Matthias A1 - Schebesta, Eduard T1 - Zertifizierung von Medizin-Software KW - Medicine KW - Software Y1 - ER - TY - GEN A1 - Sauermann, Stefan T1 - Certifying for Interoperability Update: EU Projekt Antilope, IHE Conformity Assessment Scheme Pilot KW - Interoperability Y1 - ER - TY - GEN A1 - Sauermann, Stefan T1 - Experiences from Austria: Electronic Healthcare Record ELGA - Standards and Interoperability KW - Interoperability KW - ELGA KW - Healthcare Y1 - ER - TY - GEN A1 - Sauermann, Stefan T1 - Healthcare Records in Austria and the EU Experience and projects KW - Healthcare Y1 - ER - TY - GEN A1 - Sauermann, Stefan T1 - Medizinische Langzeitregister: Erfundene Räder? KW - eHealth Y1 - ER - TY - GEN A1 - Sauermann, Stefan T1 - IEEE standards in eHealth, AAL KW - eHealth KW - IEEE standards Y1 - ER - TY - GEN A1 - Hromada, Carina A1 - Tomasch, Janine A1 - Weihs, Anna A1 - Rünzler, Dominik A1 - Teuschl, Andreas T1 - Engineering of 3D Tissue Constructs Using our Novel MagneTissue Bioreactor as Alternatives to Animal Models KW - Bioreactor KW - Biomaterials Y1 - ER - TY - GEN A1 - Sauermann, Stefan T1 - FSR Online Debate: Facilitating interoperability of energy services in Europe – (what) can we learn from existing experience?” KW - Interoperability KW - eHealth Y1 - 2020 ER - TY - GEN A1 - Litzenberger, Stefan A1 - Mally, Franziska A1 - Braunstein, Björn A1 - Willwacher, Steffen A1 - Sabo, Anton A1 - Brüggemann, Gert-Peter T1 - Influence of weighted cuffs on ground reaction forces in running of an elite unilateral upper extremity amputee athlete. KW - Amputation KW - Running Y1 - 2018 ER - TY - GEN A1 - Litzenberger, Stefan A1 - Mally, Franziska A1 - Willwacher, Steffen A1 - Braunstein, Björn A1 - Sabo, Anton A1 - Brüggemann, Gert-Peter T1 - Einfluss einer unilateralen Dysmelie des Unterarms auf die Bodenreaktionskräfte eines 800m Läufers KW - Running Y1 - 2018 ER - TY - GEN A1 - Felsner, Eduard-Max A1 - Litzenberger, Stefan A1 - Mally, Franziska A1 - Sabo, Anton T1 - Musculoskeletal modelling of elite handcycling motion: Evaluation of muscular on- and offset KW - Handcycling Y1 - 2018 ER - TY - GEN A1 - Litzenberger, Stefan A1 - Sabo, Anton A1 - Fuss, Franz Konstantin T1 - Effect of different mounting angles of prosthetic feet dedicated to sprinting on reaction forces KW - Prothesis KW - Running Y1 - 2018 ER - TY - GEN A1 - Sauermann, Stefan T1 - Telemedicine and electronic health records: Status quo and future prospects KW - eHealth Y1 - 2018 ER - TY - GEN A1 - Sauermann, Stefan T1 - Telemonitoring in der EU und international: Status und Entwicklungen KW - Telemonitoring Y1 - 2018 ER - TY - CHAP A1 - Thiele, Gerrit A1 - Kafka, Patricia A1 - Litzenberger, Stefan A1 - Sabo, Anton T1 - Neck Braces in Motocross: Different Designs and Their Effects on Muscular Activity of the Neck T2 - Procedia Engineering KW - Motocross KW - Neck Brace Y1 - 2018 SP - 71 EP - 76 ER - TY - CHAP A1 - Litzenberger, Stefan A1 - Mally, Franziska A1 - Sabo, Anton T1 - Influence of Different Seating and Crank Positions on Muscular Activity in Elite Handcycling - A Case Study T2 - Procedia Engineering KW - Handcycling KW - Muscular Activity Y1 - 2018 SP - 355 EP - 360 ER - TY - GEN A1 - Litzenberger, Stefan A1 - Mally, Franziska A1 - Sabo, Anton T1 - Influence of Different Seating and Crank Positions on Muscular Activity in Elite Handcycling - A Case Study KW - Handcycling KW - Muscular Activity Y1 - 2018 ER - TY - CHAP A1 - Mally, Franziska A1 - Litzenberger, Stefan A1 - Sabo, Anton T1 - Kinematics of Elite Unilateral Below-elbow Amputee Treadmill-running - A Case Study T2 - Procedia Engineering KW - Amputation KW - Treadmill Y1 - 2018 SP - 449 EP - 454 ER - TY - CHAP A1 - Travnicek, Peter A1 - Litzenberger, Stefan A1 - Sabo, Anton T1 - Love at First Try? How Reliable is a First Impression for Selecting a Golf Putter? T2 - Procedia Engineering KW - Golf Y1 - 2018 SP - 65 EP - 70 ER - TY - JOUR A1 - Leeb, Christine A1 - Leitner, Rita A1 - Pichler, Verena A1 - Huber-Gries, Carina A1 - Rünzler, Dominik A1 - Jesenberger, Veronika T1 - Einführung und Optimierung eines praxisorientierten PBL-Moduls im Life-Science-Bereich JF - Zeitschrift für Hochschulentwicklung (ZFHE) KW - Life Science KW - Problem Based Learning KW - Education Y1 - 2018 VL - 11 IS - 3 SP - 107 EP - 121 ER - TY - GEN A1 - Sauermann, Stefan T1 - Die Gazelle in der Krankenhaus-IT – neue Wege für die Sicherheitstechnische Kontrolle und Leistungsüberprüfung KW - Krankenhaus IT Y1 - 2020 ER - TY - GEN A1 - Sauermann, Stefan T1 - Presentation of successful use of IHE profiles in national strategies (Austria and Switzerland) KW - eHealth Y1 - 2020 ER - TY - GEN A1 - Sauermann, Stefan T1 - Interoperabilität von Prozessen und IT-Systemen im Gesundheitswesen KW - QM und Digitalisierung KW - Interoperabilität Y1 - 2020 ER - TY - GEN A1 - Sauermann, Stefan T1 - Forum Spital für Manager 2019 - Digitalisierung im Krankenhaus - Vorsitzender KW - eHealth KW - Digitalisierung im Krankenhaus Y1 - 2020 ER - TY - GEN A1 - Sauermann, Stefan T1 - Data and Integrated Health Care: � Potential and Challenges of IT innovation KW - eHealth Y1 - 2020 ER - TY - GEN A1 - Sauermann, Stefan T1 - The potential of multi-stakeholder alignment in diabetes KW - Diabetes Y1 - 2020 ER - TY - GEN A1 - Sauermann, Stefan T1 - IHE Education & IHE Internationales KW - Health Interoperability KW - eHealth Y1 - 2020 ER - TY - JOUR A1 - Rieder, Bernhard A1 - Weihs, Anna A1 - Weidinger, Adelheid A1 - Sczwarc, Dorota A1 - Nürnberger, Sylvia A1 - Redl, Heinz A1 - Rünzler, Dominik A1 - Huber-Gries, Carina A1 - Teuschl, Andreas T1 - Hydrostatic pressure-generated reactive oxygen species induce osteoarthritic conditions in cartilage pellet cultures JF - Scientific Reports KW - Bioreactor KW - Osteoarthritis KW - Cartilage KW - Reactive oxygen species Y1 - ER - TY - CHAP A1 - Gerbovics, Ferenc A1 - Frohner, Matthias A1 - Urbauer, Philipp A1 - Bruckner, Ronald A1 - Pohn, Birgit A1 - Sauermann, Stefan A1 - Mense, Alexander T1 - DEVELOPMENT AND EXTENSION OF A MODULAR, JAVA-BASED, 2ND GENERATION ISO/IEEE 11073 MANAGER FRAMEWORK T2 - Tagungsband der eHealth und eHealth Benchmarking, Wien KW - ISO KW - Java KW - eHealth Y1 - 2019 ER - TY - GEN A1 - Sauermann, Stefan A1 - Behr, Marco A1 - Lyssy, Christoph A1 - Kabakci, Müge A1 - Mense, Alexander T1 - Erstellung eines Frameworks für standarbasierte Anwendungen im Personal Healthcare KW - Healthcare KW - Standards Y1 - 2019 ER - TY - CHAP A1 - Rafolt, Dietmar A1 - Mayr, Winfried A1 - Vetter, K. A1 - Gallasch, E. A1 - Bijak, Manfred A1 - Reichel, Martin A1 - Sauermann, Stefan A1 - Lanmüller, Hermann A1 - Unger, Ewald A1 - Hofer, Christian T1 - Saturation of the M-Wave Amplitude with Increasing Stimulation Amplitude T2 - Proceedings of the 8th Vienna International Workshop on FES KW - Amplitude Y1 - 2019 SP - 122 EP - 124 ER - TY - CHAP A1 - Rathgeber, Thomas A1 - Tekautz, P. A1 - Eckelt, Markus A1 - Litzenberger, Stefan A1 - Reichel, Martin A1 - Sabo, Anton T1 - Correlation between foot pronation and overcrossing during running T2 - 1st i-FAB Congress / Proceedings of The international Foot and Ankle Biomechanics community, Bologna (Italy) KW - Sport KW - Running Y1 - 2019 ER - TY - CHAP A1 - Tekautz, P. A1 - Rathgeber, Thomas A1 - Eckelt, Markus A1 - Litzenberger, Stefan A1 - Reichel, Martin A1 - Sabo, Anton T1 - Correlation between foot pronation and toeing out during running T2 - 1st i-FAB Congress / Proceedings of The international Foot and Ankle Biomechanics community, Bologna (Italy) KW - Sport KW - Running Y1 - 2019 ER - TY - JOUR A1 - Teuschl, Andreas A1 - Zipperle, Johannes A1 - Huber-Gries, Carina A1 - Kaplan, David T1 - Silk fibroin based carrier system for delivery of fibrinogen and thrombin as coagulant supplements JF - Journal of Biomedical Materials Research KW - Fibrin KW - Silk Y1 - 2018 VL - 105 IS - 3 ER - TY - CHAP A1 - Frohner, Matthias A1 - Meyer, Markus A1 - Donsa, Klaus A1 - Urbauer, Philipp A1 - David, Veronika A1 - Sauermann, Stefan T1 - Telemonitoring of Blood Glucose - A Prototype Android Application Enhancing the Patient / Health Professional Experience Using Health IT Communication Standards T2 - DSAI 2018 - 8th International Conference on Software Development and Technologies for Enhancing Accessibility and Fighting Info-exclusion KW - Mobile Application KW - Blood Glucose KW - Telemonitoring KW - Communication Standards Y1 - ER - TY - CHAP A1 - Sauermann, Stefan A1 - Bijak, Manfred A1 - Reichel, Martin A1 - Schmutterer, Christoph A1 - Rafolt, Dietmar A1 - Unger, Ewald A1 - Mayr, Winfried A1 - Lanmüller, Hermann T1 - BioSignalizer -A Class Library for Acquisition, Analysis, Display, and Management of Biosignals in Clinical and Experimental Research T2 - Proceedings / Österreichische Computergesellschaft KW - Biosignals Y1 - 2019 SP - 143 EP - 149 ER - TY - GEN A1 - Frohner, Matthias A1 - Meyer, Markus A1 - Donsa, Klaus A1 - Urbauer, Philipp A1 - David, Veronika A1 - Sauermann, Stefan T1 - Telemonitoring of Blood Glucose - A Prototype Android Application Enhancing the Patient / Health Professional Experience Using Health IT Communication Standards KW - Mobile Application KW - Blood Glucose KW - Telemonitoring KW - Communication Standards Y1 - ER - TY - CHAP A1 - Tekautz, P. A1 - Rathgeber, P. A1 - Litzenberger, Stefan A1 - Sabo, Anton T1 - Comparison of toeing out of the foot between overground and Treadmill Running T2 - The Impact of Technology on Sport III KW - Sport KW - Running Y1 - 2019 SP - 139 EP - 143 ER - TY - GEN A1 - Sauermann, Stefan T1 - Software –eine Aufgabe für den TSB?Orientierung im Dschungel der Beauftragten! KW - eHealth KW - Krankenhaus-IT Y1 - 2020 ER - TY - JOUR A1 - Sauermann, Stefan T1 - Practical Ideas from Professors: Standards Education in Your Courses JF - IEEE Educators Resource Library KW - Education Y1 - ER - TY - JOUR A1 - Bijak, Manfred A1 - Winfried, Mayr A1 - Rakos, Monika A1 - Hofer, Christian A1 - Lanmüller, Hermann A1 - Rafolt, Dietmar A1 - Reichel, Martin A1 - Sauermann, Stefan A1 - Schmutterer, Christoph A1 - Unger, Ewald A1 - Russold, Michael A1 - Kern, Helmut T1 - The Vienna functional electrical stimulation system for restoration of walking functions in spastic paraplegia JF - Artificial Organs KW - Electrical Stimulation Y1 - 2019 IS - 26 / 3 SP - 227 ER - TY - CHAP A1 - Bittermann, Mark A1 - Butschek, Alain A1 - Engel, Raffael A1 - Haller, Michael A1 - Gerbovics, Ferenc A1 - Mense, Alexander A1 - Sauermann, Stefan T1 - DEVELOPMENT AND REALISATION OF DATA COMMUNICATION FOR AN RFID AND BLUETOOTH SUPPORTED THERMOMETER BASED ON THE ISO/IEEE 11073 STANDARD T2 - Tagungsband der eHealth und eHealth Benchmarking, Wien KW - ISO KW - Data Communication KW - RFID KW - Bluetooth Y1 - 2019 ER - TY - JOUR A1 - Thiele, Gerrit A1 - Kafka, Patricia A1 - Litzenberger, Stefan A1 - Sabo, Anton T1 - A sensor construction to measure contact between helmet and neck brace during motocross riding JF - Proceedings of the Institution of Mechanical Engineers, Part P: Journal of Sports Engineering and Technology KW - Motocross Y1 - ER - TY - CHAP A1 - Reichel, Martin A1 - Bijak, Manfred A1 - Mayr, Winfried A1 - Lanmüller, Hermann A1 - Rafolt, Dietmar A1 - Rakos, Monika A1 - Sauermann, Stefan A1 - Unger, Ewald A1 - Turkof, E. T1 - Computer Controlled Intraoperative Electroneurodiagnostics T2 - Proceedings of the World Congress on Neuroinformatics / ARGESIM/ASIM, Wien KW - Electrical Stimulation Y1 - 2019 SP - 612 EP - 616 ER - TY - CHAP A1 - Sauermann, Stefan A1 - David, Veronika A1 - Schlögl, Alois A1 - Egelkraut, Reinhard A1 - Frohner, Matthias A1 - Pohn, Birgit A1 - Urbauer, Philipp A1 - Mense, Alexander T1 - Biosignals, Standards and FHIR – The Way to Go? T2 - Stud Health Technol Inform KW - Biosignals KW - Standards Y1 - 2018 SP - 356 EP - 362 ER - TY - CHAP A1 - Gerbovics, Ferenc A1 - Frohner, Matthias A1 - Urbauer, Philipp A1 - Pohn, Birgit A1 - Bruckner, Ronald A1 - Sauermann, Stefan A1 - Mense, Alexander T1 - Interoperabilität von medizinischen Geräten T2 - Tagungsband des 4. Forschungsforum der österreichischen Fachhochschulen, Pinkafeld KW - Interoperability KW - Medical devices Y1 - 2019 SP - 437 EP - 439 ER - TY - CHAP A1 - Oertel, Clemens A1 - Litzenberger, Stefan A1 - Eckelt, Markus A1 - Kafka, Patricia A1 - Sabo, Anton T1 - Development of a flexible Shot-Put Chair-Back for an Elite Paralympics Athlete T2 - The Impact of Technology on Sport III KW - Sport KW - Paralympics Y1 - 2019 SP - 429 EP - 433 ER - TY - JOUR A1 - Litzenberger, Stefan A1 - Sabo, Anton T1 - Can Silicon Retina Sensors be used for optical motion analysis in sports? JF - Procedia Engineering KW - Sport KW - Sensor Y1 - 2019 VL - 2012 IS - Vol 34/ No. 0 ER - TY - CHAP A1 - Behr, Marco A1 - Lyssy, Christoph A1 - Kabakci, Müge A1 - Mense, Alexander A1 - Sauermann, Stefan T1 - Archivierung von standardisierten IEEE 11073 Informationsobjekten in einer Datenbank für den Personal Healthcare T2 - Tagungsband / 3. Forschungsforum der österreichischen Fachhochschulen, Villach (Österreich) KW - Healthcare KW - Standards Y1 - 2019 ER - TY - CHAP A1 - Litzenberger, Stefan A1 - Fechter, Andreas A1 - Balac, Mathias A1 - Pfeifer, Mafred A1 - Reichel, Martin A1 - Sabo, Anton T1 - Einfluss der Pedalfußposition auf ausgewählte Parameter beim Mountainbiken T2 - Sporttechnologie zwischen Theorie und Praxis IV KW - Sport KW - Montainbiking Y1 - 2019 SP - 363 EP - 375 PB - Shaker Verlag CY - Aachen ER - TY - JOUR A1 - Sleytr, Uwe B. A1 - Huber-Gries, Carina A1 - Nicola, Ilk A1 - Puhm, Dietmar A1 - Schuster, Bernhard A1 - Egelseer, Eva-Maria T1 - S-layers as a tool kit for nanobiotechnological applications JF - FEMS Microbiology Letters KW - Nanobiotechnology Y1 - 2019 IS - 267(2) ER - TY - GEN A1 - Kamravamanesh, Donya A1 - Pflügl, Stefan A1 - Herwig, Christoph A1 - Lackner, Maximilian T1 - Optimization of process parameters to enhance PHA accumulation in Synechosystis sp. PCC 6714 using multivariate design of experiments KW - Bacteria Y1 - 2018 ER - TY - JOUR A1 - Pasching, Emrich A1 - Eckehard, Diem A1 - Litzenberger, Stefan A1 - Sabo, Anton T1 - Falls into via ferrata climbing sets carry a higher injury risk for lightweight climbers JF - Proceedings of the Institution of Mechanical Engineers, Part P: Journal of Sports Engineering and Technology KW - Sport KW - Climbing Y1 - 2019 ER - TY - GEN A1 - Sauermann, Stefan T1 - IHE / Compliance - Interoperabilität und Hygiene – gibt es Gemeinsamkeiten? KW - Telemonitoring KW - ELGA KW - Interoperability KW - Standardization Y1 - ER - TY - CHAP A1 - Rafolt, Dietmar A1 - Mayr, Winfried A1 - Freilinger, G. A1 - Bijak, Manfred A1 - Lanmüller, Hermann A1 - Reichel, Martin A1 - Sauermann, Stefan A1 - Schmutterer, Christoph A1 - Unger, Ewald A1 - Kozlovskaya, I. T1 - Application of Functional Electrical Stimulation (FES) for training in Long-Term Space Flights T2 - Proceedings CD / 18.Congress of the International Society of Biomechanics, Zürich KW - Electrical Stimulation Y1 - 2019 ER - TY - CHAP A1 - Sauermann, Stefan A1 - Bijak, Manfred A1 - Rafolt, Dietmar A1 - Unger, Ewald A1 - Reichel, Martin A1 - Mayr, Winfried A1 - Lanmüller, Hermann T1 - The BioSignalizer Software for Experimental Work on Functional Electrostimulation of Long-Term Denervated Rabbit Muscle T2 - Proceedings of the World Congress on Medical Physics and Biomedical Engineering (IFMBE 2003) KW - Muscle Stimulation KW - Electrical Stimulation KW - Muscle Denervation Y1 - 2019 ER - TY - JOUR A1 - Pillmeier, Christoph A1 - Litzenberger, Stefan A1 - Sabo, Anton T1 - The effect of on-deck warm-up routines in baseball on bat velocity, muscular activity and intensity in time-frequency space JF - Procedia Engineering KW - Sport KW - Baseball Y1 - 2019 IS - Vol 34/ No. 0 SP - 230 EP - 235 ER - TY - GEN A1 - Behr, Marco A1 - Lyssy, Christoph A1 - Kabakci, Müge A1 - Mense, Alexander A1 - Sauermann, Stefan T1 - Archivierung von standardisierten IEEE 11073 Informationsobjekten in einer Datenbank für den Personal Healthcare KW - Healthcare KW - Standards Y1 - 2019 ER - TY - CHAP A1 - Sauermann, Stefan A1 - Bijak, Manfred A1 - Rafolt, Dietmar A1 - Unger, E. A1 - Reichel, Martin A1 - Mayr, Winfried A1 - Lanmüller, Hermann T1 - Automated Equipment for electrical stimulation and analysis of the contraction dynamics of electrically stimulated skeletal muscle T2 - Proceedings of the 17th ISOB, Brisbane KW - Electrical Stimulation KW - Muscle Stimulation Y1 - 2019 SP - 53 EP - 54 ER - TY - CHAP A1 - Sauermann, Stefan A1 - Bijak, Manfred A1 - Reichel, Martin A1 - Rafolt, Dietmar A1 - Unger, Ewald A1 - Mayr, Winfried A1 - Lanmüller, Hermann T1 - Automated Estimation of Stimulation Threshold using Single Twitches and Acceleration Sensors T2 - Proceedings / Department of Biomedical Engineering and Physics, University of Vienna KW - Electrical Stimulation Y1 - 2019 SP - 148 EP - 151 ER - TY - JOUR A1 - Saravia, Veronica A1 - Nolte, M. A1 - Huber-Gries, Carina A1 - Puhm, Dietmar A1 - Fery, Andreas A1 - Sleytr, Uwe B. A1 - Toca-Herrera, Jose Luis T1 - Bacterial protein patterning by micro-contact printing of PLL-g-PEG JF - Journal of Biotechnology KW - Proteins Y1 - 2019 IS - 130(3) SP - 247 EP - 252 ER - TY - CHAP A1 - Sauermann, Stefan A1 - Bijak, Manfred A1 - Rafolt, Dietmar A1 - Unger, Ewald A1 - Reichel, Martin A1 - Mayr, Winfried A1 - Lanmüller, Hermann T1 - Automatisierte Datenerfassung zur Bestimmung optimaler FES Parameter T2 - Biomedizinische Technik 48. Ergänzungsband 1 KW - Data Collection Y1 - 2019 SP - 50 EP - 51 ER - TY - JOUR A1 - Thiele, Gerrit A1 - Kafka, Patricia A1 - Litzenberger, Stefan A1 - Sabo, Anton T1 - A sensor construction to measure contact between helmet and neck brace during motocross riding. JF - Journal of Sports Engineering and Technology KW - Motocross Y1 - 2018 ER - TY - CHAP A1 - Reichel, Martin A1 - Bijak, Manfred A1 - Mayr, Winfried A1 - Lanmüller, Hermann A1 - Rafolt, Dietmar A1 - Sauermann, Stefan A1 - Unger, Ewald A1 - Turkof, E. T1 - Biphasic Stimulation: An Alternative Approach to Minimize the Stimulus Artifact for Diagnostic and for Control Applications T2 - Proceedings of the 7th Vienna International Workshop on FES / Department of Biomedical Engineering and Physics, University of Vienna KW - Electrical Stimulation Y1 - 2019 SP - 122 EP - 125 ER - TY - CHAP A1 - Frohner, Matthias A1 - Urbauer, Philipp A1 - Bauer, Martin A1 - Gerbovics, Ferenc A1 - Mense, Alexander A1 - Sauermann, Stefan T1 - Design and Realisation of a Framework for Device Endcommunication According to the IEEE 11073-20601 Standard T2 - Tagungsband der eHealth und eHealth Benchmarking, Wien KW - Standards KW - eHealth KW - IEEE Y1 - 2019 ER - TY - GEN A1 - Litzenberger, Stefan A1 - Christensen, Troels A1 - Hofstätter, Otto A1 - Sabo, Anton T1 - "Klassifizierung des Straßenzustands beim Radfahren mittels Smartphone-Daten" KW - Cycling KW - Road Surface KW - Machine Learning KW - Accelerometer Data Y1 - ER - TY - GEN A1 - Litzenberger, Stefan A1 - Christensen, Troels A1 - Hofstätter, Otto A1 - Sabo, Anton T1 - Prediction of Road Surface Quality during Cycling Using Smartphone Accelerometer Data KW - Cycling KW - Road Surface KW - Machine Learning KW - Accelerometer Data Y1 - ER - TY - GEN A1 - Sauermann, Stefan T1 - Flashlight: Telemonitoring Rahmenrichtlinie KW - Telemonitoring KW - ELGA KW - Standardization KW - Interoperability KW - Telehealth Y1 - ER - TY - CHAP A1 - Mayr, Winfried A1 - Freilinger, G. A1 - Rafolt, Dietmar A1 - Bijak, Manfred A1 - Girsch, Werner A1 - Hofer, Christian A1 - Lanmüller, Hermann A1 - Reichel, Martin A1 - Sauermann, Stefan A1 - Schmutterer, Christoph A1 - Unger, Ewald A1 - Gratchev, V. T1 - FES as a Countermeasure against Muscular Atrophy in Long-Term Space Flights T2 - 7th Vienna International Workshop on FES / Department of Biomedical Engineering and Physics University of Vienna KW - Electrical Stimulation KW - Muscle Stimulation Y1 - 2019 SP - 246 EP - 249 ER - TY - JOUR A1 - Wurnitsch, Wolfgang A1 - Siebert, Marc A1 - Litzenberger, Stefan A1 - Sabo, Anton T1 - Development of an individually customizable integral carbon aerobar based on sEMG measurements of the upper limbs JF - Procedia Engineering KW - Sport Y1 - 2019 VL - 2010 IS - Vol. 2 (2) SP - 2631 EP - 2635 ER - TY - CHAP A1 - Graf, Simon A1 - Litzenberger, Stefan A1 - Sabo, Anton T1 - Edging stiffness of ski touring bindings T2 - Procedia Engineering KW - Sport KW - Ski Touring Y1 - 2019 SP - 37 EP - 43 ER - TY - GEN A1 - Sauermann, Stefan T1 - Continua Guidelines: Aktueller Stand und Ausblick KW - Standardization KW - eHealth Y1 - ER - TY - CHAP A1 - Leeb, Christine A1 - Leitner, Rita A1 - Pichler, Verena A1 - Huber-Gries, Carina A1 - Rünzler, Dominik A1 - Jesenberger, Veronika T1 - Einführung und Optimierung eines praxisorientierten PBL-Moduls im Life-Science-Bereich T2 - Proceedings des Kongresses Problem-Based Learning 2016 PBL - Kompetenzen fördern, Zukunft gestalten KW - Life Science KW - Problem Based Learning KW - Education Y1 - 2018 ER - TY - CHAP A1 - Gastinger, Roman A1 - Litzenberger, Stefan A1 - Sabo, Anton T1 - Design, development and construction of a monitoring table tennis net T2 - Procedia Engineering KW - Sport KW - Tennis Y1 - 2019 SP - 297 EP - 303 ER - TY - GEN A1 - Sauermann, Stefan A1 - Frohner, Matthias T1 - Wie vernetzen wir Medizintechnik und IT in OP und ICU KW - Surgery KW - Intensive Care unit KW - Standardization KW - Telemonitoring KW - Digital Healthcare Y1 - ER - TY - JOUR A1 - Mally, Franziska A1 - Litzenberger, Stefan A1 - Willwacher, Steffen A1 - Braunstein, Björn A1 - Brüggemann, Gert-Peter A1 - Sabo, Anton T1 - Kinetics of elite unilateral belowelbow amputee running: Comparison of symmetry of an impaired and an unimpaired athlete and the influence of additional weight on the impaired limb JF - Sports Engineering KW - Amputation KW - Athletes Y1 - 2018 ER - TY - GEN A1 - Leeb, Christine A1 - Leitner, Rita A1 - Pichler, Verena A1 - Huber-Gries, Carina A1 - Rünzler, Dominik A1 - Jesenberger, Veronika T1 - Einführung und Optimierung eines praxisorientierten PBL-Moduls im Life-Science-Bereich KW - Life Science KW - Problem Based Learning KW - Education Y1 - 2018 ER - TY - JOUR A1 - Kamravamanesh, Donya A1 - Pflügl, Stefan A1 - Nischkauer, Winfried A1 - Limbeck, Andreas A1 - Lackner, Maximilian A1 - Herwig, Christoph T1 - Photosynthetic poly-β-hydroxybutyrate accumulation in unicellular cyanobacterium Synechocystis sp. PCC 6714 JF - AMB Express KW - Bacteria Y1 - 2018 VL - 143 IS - 7 ER - TY - GEN A1 - Sauermann, Stefan T1 - Digital Healthcare als Werkzeug für die nahtlose Integrierte Versorgung KW - Telemedicine KW - Telehealth KW - Decision Making KW - ELGA KW - Standardization Y1 - ER - TY - GEN A1 - Sauermann, Stefan T1 - Die digitale Revolution und ihre Auswirkungen auf die Gesellschaft KW - Interoperability KW - Standardization KW - eHealth KW - ELGA KW - Digital Healthcare Y1 - ER - TY - JOUR A1 - Sauermann, Stefan T1 - RDA COVID-19 Working Group. Recommendations and Guidelines on data sharing JF - Research Data Alliance, 2020. N2 - The Research Data Alliance (RDA) COVID-19 Working Group members bring various, global expertise to develop a body of work that comprises how data from multiple disciplines inform response to a pandemic combined with guidelines and recommendations on data sharing under the present COVID-19 cicumstances. This extends to research software sharing, in recognition of the key role in software in analysing data. The work has been divided into four research areas (namely, clinical, omics, epidemiology, social sciences) with four cross cutting themes (namely, community participation, indigenous data, legal and ethical considerations, research software), as a way to focus the conversations, and provide an initial set of guidelines in a tight timeframe. The detailed guidelines are aimed to help stakeholders follow best practices to maximise the efficiency of their work, and to act as a blueprint for future emergencies. The recommendations in the document are aimed at helping policymakers and funders to maximise timely, quality data sharing and appropriate responses in such health emergencies. This work was executed in an intense period over just over 6 weeks, with five iterations, all of which were opened for public community comment. Draft releases and comments are avaialable here (https://doi.org/10.15497/rda00046). This activity has been conducted under the RDA guiding principles of Openness, Consensus, Balance, Harmonization, Community-driven, and Non-profit and technology-neutral. KW - COVID19 Data Sharing Y1 - 2020 ER - TY - JOUR A1 - David, Veronika A1 - Jagos, Harald A1 - Litzenberger, Stefan A1 - Reichel, Martin T1 - Instrumented insole for mobile and long distance motion pattern measurement JF - Procedia Engineering KW - Health data KW - Insole Y1 - 2019 IS - Vol 34/no 0 SP - 760 EP - 765 ER - TY - CHAP A1 - Reichel, Martin A1 - Bijak, Manfred A1 - Mayr, Winfried A1 - Lanmüller, Hermann A1 - Rafolt, Dietmar A1 - Rakos, Monika A1 - Sauermann, Stefan A1 - Unger, Ewald A1 - Turkof, E. T1 - Mobile PC-System for Intraoperative Electroneurodiagnostics T2 - Proceedings of the 7th Vienna International Workshop on FES / Vienna, Austria, Department of Biomedical Engineering and Physics, University of Vienna KW - Electroneurodiagnostics Y1 - 2019 SP - 250 EP - 253 ER - TY - CHAP A1 - Sauermann, Stefan A1 - Bijak, Manfred A1 - Reichel, Martin A1 - Unger, Ewald A1 - Rakos, Monika A1 - Rafolt, Dietmar A1 - Mayr, Winfried A1 - Lanmüller, Hermann T1 - A Computerized System for Control, Data Aquisition and Online Analyses of Biosignals T2 - Proceedings ARGESIM/ASIM, Wien KW - Biosignals Y1 - 2019 SP - 608 EP - 611 ER - TY - CHAP A1 - Bijak, Manfred A1 - Mayr, Winfried A1 - Rakos, Monika A1 - Hofer, Christian A1 - Lanmüller, Hermann A1 - Rafolt, Dietmar A1 - Reichel, Martin A1 - Sauermann, Stefan A1 - Schmutterer, Christoph A1 - Unger, Ewald A1 - Russold, Michael A1 - Kern, Helmut T1 - The Vienna FES system for restoration of walking functions in spastic paraplegia T2 - Proceedings of the 7th Vienna International Workshop on Functional Electrical Stimulation KW - Electrical Stimulation Y1 - 2019 ER - TY - GEN A1 - Reichel, Martin A1 - Martinek, Johannes A1 - Sauermann, Stefan A1 - Schmöllebeck, Fritz T1 - eHealth und eCard an der Fachhochschule Technikum Wien KW - eHealth Y1 - 2019 ER - TY - JOUR A1 - Litzenberger, Stefan A1 - Mally, Franziska A1 - Sabo, Anton T1 - Biomechanics of elite recumbent handcycling: a case study JF - Sports Engineering KW - Handcycling Y1 - 2018 ER - TY - JOUR A1 - Tang, Jilin A1 - Ebner, Andreas A1 - Ilk, Nicola A1 - Lichtblau, Helga A1 - Huber-Gries, Carina A1 - Zhu, Rong A1 - Pum, Dietmar A1 - Leitner, Michael A1 - Pastushenko, Vassili Filippovich A1 - Gruber, Hermann A1 - Sleytr, Uwe B. A1 - Hinterdorfer, Peter T1 - High-affinity tags fused to s-layer proteins probed by atomic force microscopy JF - Langmuir KW - Proteins Y1 - 2019 IS - 24(4) SP - 1324 EP - 1329 ER - TY - CHAP A1 - Sabutsch, Stefan A1 - Mense, Alexander A1 - Sauermann, Stefan T1 - Development of a nationwide harmonized interoperable laboratory report based on CDA for the Austrian Electronic Health Record System T2 - Proceedings of the 7th International Conference on Information Communication Technologies in Health 2009, Greece KW - Electronic Health Records KW - Interoperability Y1 - 2019 SN - 978-960-466-042-1 SN - 1790-3904 ER - TY - GEN A1 - Forjan, Mathias T1 - Reference Architecture and IT Standards for Integrating Medical Devices KW - Electronic Health Records Y1 - 2018 ER - TY - JOUR A1 - Pasteka, Richard A1 - Santos da Costa, Joao Pedro A1 - Barros, Nelson A1 - Kolar, Radim A1 - Forjan, Mathias T1 - Patient–Ventilator Interaction Testing Using the Electromechanical Lung Simulator xPULMTM during V/A-C and PSV Ventilation Mode JF - Applied Sciences N2 - During mechanical ventilation, a disparity between flow, pressure and volume demands of the patient and the assistance delivered by the mechanical ventilator often occurs. This paper introduces an alternative approach of simulating and evaluating patient–ventilator interactions with high fidelity using the electromechanical lung simulator xPULM™. The xPULM™ approximates respiratory activities of a patient during alternating phases of spontaneous breathing and apnea intervals while connected to a mechanical ventilator. Focusing on different triggering events, volume assist-control (V/A-C) and pressure support ventilation (PSV) modes were chosen to test patient–ventilator interactions. In V/A-C mode, a double-triggering was detected every third breathing cycle, leading to an asynchrony index of 16.67%, which is classified as severe. This asynchrony causes a significant increase of peak inspiratory pressure (7.96 ± 6.38 vs. 11.09 ± 0.49 cmH2O, p < 0.01)) and peak expiratory flow (−25.57 ± 8.93 vs. 32.90 ± 0.54 L/min, p < 0.01) when compared to synchronous phases of the breathing simulation. Additionally, events of premature cycling were observed during PSV mode. In this mode, the peak delivered volume during simulated spontaneous breathing phases increased significantly (917.09 ± 45.74 vs. 468.40 ± 31.79 mL, p < 0.01) compared to apnea phases. Various dynamic clinical situations can be approximated using this approach and thereby could help to identify undesired patient–ventilation interactions in the future. Rapidly manufactured ventilator systems could also be tested using this approach. View Full-Text KW - Breathing Simulation KW - Biomedical Engineering KW - Electromechanical lung simulator Y1 - VL - 11 IS - 9 ER - TY - GEN A1 - Frohner, Matthias A1 - Forjan, Mathias T1 - Interoperability in the OR Introducing OR.NET KW - Interoperability KW - Emergency Room KW - Harmonization Y1 - ER - TY - CHAP A1 - Frohner, Matthias A1 - Windisch, Michael A1 - Sauermann, Stefan A1 - Sekora, Jiri A1 - Forjan, Mathias T1 - Organ Telemonitoring in Ex-vivo Nutrition Circulation of Porcine Lungs Using Interoperability Standards T2 - Proceedings of the 12th IFAC/IEEE International Conference on Programmable Devices and Embedded Systems (PDeS 2013) KW - Telemonitoring KW - Lung Simulator KW - Interoperability Y1 - 2018 SP - 335 EP - 340 ER - TY - GEN A1 - Tabakovic, Momir A1 - Wahl, Harald A1 - Fechner, Hubert A1 - Bululukova, Darya A1 - Ballner, Mathias A1 - Allmayer, Sandra A1 - Franz, Peter A1 - Essl, Günter T1 - European Academic Smart Cities Network KW - Smart City KW - Academic Network KW - Europe Y1 - 2018 ER - TY - CHAP A1 - Lenz, Gregor A1 - Frohner, Matthias A1 - Sauermann, Stefan A1 - Forjan, Mathias T1 - LUMOR: An App for Standardized Control and Monitoring of a Porcine Lung and its Nutrient Cycle T2 - Proceedings of eHealth 2014 - Health Informatics Meets Informatics KW - Lung Simulator Y1 - 2018 ER - TY - CHAP A1 - Forjan, Mathias A1 - Frohner, Matthias T1 - Development of the mCM - mobile circulatory module - for ex-vivo physiological tissue for breathing simulation T2 - Abstracts of the 9th World Congress on Alternatives and Animal Use in the Life Sciences (ALTEX Proceedings) 2014 KW - Lung Simulator KW - Breathing Y1 - 2018 SP - 52 EP - 52 ER - TY - GEN A1 - Lenz, Gregor A1 - Frohner, Matthias A1 - Sauermann, Stefan A1 - Forjan, Mathias T1 - LUMOR: An App for Standardized Control and Monitoring of a Porcine Lung and its Nutrient Cycle KW - Lung Simulator Y1 - 2018 ER - TY - CHAP A1 - Urbauer, Philipp A1 - Herzog, Juliane A1 - Pohn, Birgit A1 - Forjan, Mathias A1 - Sauermann, Stefan T1 - Certification Programs for eHealth - Status Quo T2 - Proceedings of eHealth 2014 - Health Informatics Meets Informatics KW - eHealth KW - Education Y1 - 2018 ER - TY - GEN A1 - Forjan, Mathias A1 - Frohner, Matthias T1 - Development of the mCM - mobile circulatory module - for ex-vivo physiological tissue for breathing simulation KW - Lung Simulator KW - Breathing Y1 - 2018 ER - TY - GEN A1 - David, Veronika A1 - Forjan, Mathias A1 - Tkachenko Bril, Andres Igor T1 - Klinische Pilotstudie zur Prüfung der Machbarkeit und Akzeptanz eines Biofeedbacksystems zur Unterstützung der teilbelastenden Mobilisation KW - eHealth Y1 - 2018 ER - TY - GEN A1 - Sauermann, Stefan A1 - Forjan, Mathias A1 - Frohner, Matthias T1 - Integrating medical devices in hospitals and at home: Challenges & potentials KW - Electronic Health Records Y1 - 2018 ER - TY - GEN A1 - Sauermann, Stefan A1 - Forjan, Mathias T1 - eHealth Strategies on the Move: Researching State of the Art KW - eHealth Y1 - 2018 ER - TY - CHAP A1 - David, Veronika A1 - Forjan, Mathias A1 - Scherer, Matthias A1 - Reichel, Martin T1 - Entwicklung und Vorstudien zur Implementierung mobiler Rehabilitationssysteme im häuslichen Umfeld T2 - 9. Jahrestagung der deutschen Gesellschaft für Biomechanik (DgfB) 2015 KW - Rehabilitation Y1 - ER - TY - GEN A1 - David, Veronika A1 - Forjan, Mathias A1 - Scherer, Matthias A1 - Reichel, Martin T1 - Entwicklung und Vorstudien zur Implementierung mobiler Rehabilitationssysteme im häuslichen Umfeld KW - Rehabilitation Y1 - ER - TY - CHAP A1 - Pucher, Robert A1 - Wahl, Harald A1 - Mense, Alexander A1 - Nimmervoll, Alexander A1 - Hofmann, Alexander A1 - Schmöllebeck, Fritz T1 - Didaktik für das Wissenszeitalter T2 - Beiträge der Informationstagung Mikroelektronik 2003 / Schriftenreihe der ÖVE Nr. 33 KW - Didactics KW - Teaching Y1 - 2019 SP - 461 EP - 467 ER - TY - CHAP A1 - David, Veronika A1 - Forjan, Mathias A1 - Martinek, Johannes A1 - Kotzian, Stefan A1 - Jagos, Harald A1 - Rafolt, Dietmar T1 - Evaluation of Wearable Multimodal Sensor Insoles for Motion-pattern Measurements in Stroke Rehabilitation – a Pilot Study T2 - IEEE-RAS-EMBS International Conference on Rehabilitation Robotics (ICORR 2017) KW - Stroke Patients KW - Rehabilitation Y1 - 2018 ER - TY - RPRT A1 - Pasteka, Richard T1 - Der Standard, "Eine Lunge, die Beatmungsgeräte testet", 2019, Vienna, Austria. KW - Lungensimulation KW - Biomedical Engineering Y1 - 2020 ER - TY - BOOK A1 - Pasteka, Richard A1 - Hrušková, Jana A1 - Jakubík, Juraj A1 - Hendrych, Michal A1 - Svačinová, Jana A1 - Nováková, Zuzana A1 - Vejtasová, Veronika A1 - Kujalová, Veronika A1 - Budinskaya, Ksenia T1 - Fyziologie – teorie k praktickým cvičením T1 - Physiologie - Theorie zu praktischen Übungen KW - Physiology Y1 - N1 - Erstellt in Zusammenarbeit mit dem Servicezentrum für e-Learning der Masarykova univerzita PB - Masarykova univerzita, Fakulta informatiky Masarykovy univerzity CY - Brno ER - TY - GEN A1 - Pasteka, Richard T1 - International Lecture, University of Trás-os-Montes and Alto Douro, Portugal, "Human Respiratory System Modelling" KW - Biomedical Engineering Y1 - 2020 ER - TY - GEN A1 - David, Veronika A1 - Forjan, Mathias T1 - User Centered Design - Einbindung und Nutzung in Forschungsprojekten KW - Research Project KW - User Centered Design Y1 - 2018 ER - TY - GEN A1 - Urbauer, Philipp A1 - Frohner, Matthias A1 - Forjan, Mathias A1 - Pohn, Birgit A1 - Sauermann, Stefan A1 - Mense, Alexander T1 - A Closer Look on Standards Based Personal Health Device Communication: A Résumé over Four Years Implementing Telemonitoring Solutions KW - eHealth KW - Telemonitoring Y1 - 2019 ER - TY - BOOK A1 - Hrušková, Jana A1 - Pasteka, Richard A1 - Jakubík, Juraj A1 - Hendrych, Michal A1 - Svačinová, Jana A1 - Nováková, Zuzana A1 - Vejtasová, Veronika A1 - Kujalová, Veronika A1 - Budinskaya, Ksenia T1 - Workbook fyziologie protokoly BT - Biomedicínská techniky a nelékařské obory KW - Physiology Y1 - SN - 978-80-210-9897-8 PB - Masarykova univerzita CY - Brno ER - TY - CHAP A1 - Herzog, Juliane A1 - Pohn, Birgit A1 - Forjan, Mathias A1 - Sauermann, Stefan A1 - Urbauer, Philipp T1 - Education for eHealth - A Status Analysis T2 - Proceedings of eHealth 2014 - Health Informatics Meets Informatics KW - eHealth KW - Education Y1 - 2018 ER - TY - CHAP A1 - Pasteka, Richard A1 - Forjan, Mathias T1 - Actively breathing mechanical lung simulator development and preliminary measurements T2 - IFMBE,volume 65; EMBEC & NBC 2017 KW - Biomedical Engineering KW - mechanical lung-simulator Y1 - ER - TY - CHAP A1 - Pasteka, Richard A1 - Forjan, Mathias A1 - Drauschke, Andreas T1 - Comparison of Mathematical and Controlled Mechanical Lung Simulation in Active Breathing and Ventilated State T2 - Proceedings of the 15th IFAC Conference on Programmable Devices and Embedded Systems PDeS 2018 KW - Breathing Simulation KW - Mathematical Models KW - In-silico Models KW - Flow Measurement KW - Mechanical Simulation Y1 - ER - TY - CHAP A1 - Mense, Alexander A1 - Sauermann, Stefan A1 - Wahl, Harald A1 - Pucher, Robert A1 - Oeser, Reinhard T1 - Probing the Compatibility of CEN 13606 Part 4 and 5 with IHE T2 - Proceedings of the 5th International Conference On Information Communication Technologies in Health (ICICTH) / Samos Island (Greece) KW - CEN KW - Compatibility Y1 - 2019 SP - 68 EP - 73 ER - TY - JOUR A1 - Pasteka, Richard A1 - Schöllbauer, Lara Alina A1 - Santos da Costa, Joao Pedro A1 - Kolar, Radim A1 - Forjan, Mathias T1 - Experimental Evaluation of Dry Powder Inhalers During In- and Exhalation Using a Model of the Human Respiratory System (xPULM™) JF - Pharmaceutics N2 - Dry powder inhalers are used by a large number of patients worldwide to treat respiratory diseases. The objective of this work is to experimentally investigate changes in aerosol particle diameter and particle number concentration of pharmaceutical aerosols generated by four dry powder inhalers under realistic inhalation and exhalation conditions. To simulate patients undergoing inhalation therapy, the active respiratory system model (xPULM™) was used. A mechanical upper airway model was developed, manufactured, and introduced as a part of the xPULM™ to represent the human upper respiratory tract with high fidelity. Integration of optical aerosol spectrometry technique into the setup allowed for evaluation of pharmaceutical aerosols. The results show that there is a significant difference (p < 0.05) in mean particle diameter between inhaled and exhaled particles with the majority of the particles depositing in the lung, while particles with the size of (>0.5 μm) are least influenced by deposition mechanisms. The fraction of exhaled particles ranges from 2.13% (HandiHaler®) over 2.94% (BreezHaler®), and 6.22% (Turbohaler®) to 10.24% (Ellipta®). These values are comparable to previously published studies. Furthermore, the mechanical upper airway model increases the resistance of the overall system and acts as a filter for larger particles (>3 μm). In conclusion, the xPULM™ active respiratory system model is a viable option for studying interactions of pharmaceutical aerosols and the respiratory tract regarding applicable deposition mechanisms. The model strives to support the reduction of animal experimentation in aerosol research and provides an alternative to experiments with human subjects. KW - Biomedical Engineering KW - Dry powder inhaler resistance KW - optical aerosol spectrometry KW - mechanical upper airway model KW - inspiratory flow rate Y1 - 2022 VL - 2022 IS - 14/3 ER - TY - JOUR A1 - Pasteka, Richard A1 - Forjan, Mathias A1 - Sauermann, Stefan A1 - Drauschke, Andreas T1 - Electro-mechanical Lung Simulator Using Polymer and Organic Human Lung Equivalents for Realistic Breathing Simulation JF - Scientific Reports N2 - Simulation models in respiratory research are increasingly used for medical product development and testing, especially because in-vivo models are coupled with a high degree of complexity and ethical concerns. This work introduces a respiratory simulation system, which is bridging the gap between the complex, real anatomical environment and the safe, cost-effective simulation methods. The presented electro-mechanical lung simulator, xPULM, combines in-silico, ex-vivo and mechanical respiratory approaches by realistically replicating an actively breathing human lung. The reproducibility of sinusoidal breathing simulations with xPULM was verified for selected breathing frequencies (10–18 bpm) and tidal volumes (400–600 ml) physiologically occurring during human breathing at rest. Human lung anatomy was modelled using latex bags and primed porcine lungs. High reproducibility of flow and pressure characteristics was shown by evaluating breathing cycles (nTotal = 3273) with highest standard deviation |3σ| for both, simplified lung equivalents (μV˙ = 23.98 ± 1.04 l/min, μP = −0.78 ± 0.63 hPa) and primed porcine lungs (μV˙ = 18.87 ± 2.49 l/min, μP = −21.13 ± 1.47 hPa). The adaptability of the breathing simulation parameters, coupled with the use of porcine lungs salvaged from a slaughterhouse process, represents an advancement towards anatomically and physiologically realistic modelling of human respiration. KW - Breathing Simulation KW - Lung Simulator KW - Biomedical Engineering Y1 - 2020 VL - Vol 9 IS - No. 1 SP - Article number: 19778 ER - TY - GEN A1 - Pasteka, Richard T1 - International Week Turku, "Hands-on learning and research applications" KW - Biomedical Engineering Y1 - 2020 ER - TY - GEN A1 - Pasteka, Richard A1 - Forjan, Mathias A1 - Drauschke, Andreas T1 - Comparison of Mathematical and Controlled Mechanical Lung Simulation in Active Breathing and Ventilated State KW - Breathing Simulation KW - Flow Measurement KW - In-silico Models KW - Mathematical Models KW - Mechanical Simulation Y1 - ER - TY - GEN A1 - Herzog, Juliane A1 - Pohn, Birgit A1 - Forjan, Mathias A1 - Sauermann, Stefan A1 - Urbauer, Philipp T1 - Education for eHealth - A Status Analysis KW - eHealth KW - Education Y1 - 2018 ER - TY - CHAP A1 - Pasteka, Richard A1 - Santos da Costa, Joao Pedro A1 - Forjan, Mathias T1 - Characteristic Waveforms for Testing of Medical Aerosol Inhalers T2 - 8th European Medical and Biological Engineering Conference N2 - Respiratory diseases are characterised by high prevalence among the European population. Medical aerosol inhalers are the most commonly used means of drug delivery into the human respiratory system. This paper focuses on characteristic waveforms that can be utilised during aerosol deposition studies to simulate conditions of rapid human inhalation. Additionally, an inhalatory waveform based on clinically recorded spirometry data is introduced. Experimental measurements are performed and simulation results mutually compared using the electro-mechanical lung simulator xPULM. The inhalatory waveforms are repeatably simulated with high fidelity in regards to the waveform shape with the lowest value of the Goodness of fit 0.89. Additionally, the measured values for all characteristic inhalatory parameters are simulated with low standard deviation < 1. The differences between the required and measured waveform shapes are small, < 3 L/min and do not influence the overall inhalatory volume. This opens a possibility of utilising the xPULM for medical aerosol inhalers testing. KW - Breathing Simulation KW - Biomedical Engineering Y1 - SP - 240 EP - 246 PB - Springer International Publishing ER - TY - CHAP A1 - Pasteka, Richard A1 - Forjan, Mathias T1 - Changes of particle deposition caused by different breathing patterns during active lung simulation T2 - 41st Annual International Conference of the IEEE Engineering in Medicine and Biology Society (EMBC) N2 - Aerosols are an integral part of everyday life and as such are inhaled under various conditions and circumstances. These may vary based on the health and activity status of an individual. The aim of this work is to analyse the particle deposition mechanisms during the simulation of three different breathing patterns using an aerosol representing the PM1 fraction of fine particles. The active electro-mechanical lung simulator xPULM is utilized as a driving force and is combined with a non-invasive direct reading optical aerosol measurement system. Results show differences between the number of deposited particles for the three breathing patterns and for the three typical size ranges of airborne particles. Overall, the presented approach demonstrates the possibility of determining the changes of aerosol uptake based on different breathing patterns using the electro-mechanical lung simulator and laboratory produced aerosols. Further measurement cycles must be performed in order to validate the found interactions and to characterize the major influencing parameters. KW - Biomedical Engineering, Breathing simulation Y1 - ER - TY - JOUR A1 - Riess, Bernhard A1 - David, Veronika A1 - Scherer, Matthias A1 - Kotzian, Stefan A1 - Forjan, Mathias T1 - Development and Usability Test of an Innovative Low-Cost Rehabilitation Game for the Upper Extremities of Neurological Patients JF - Journal of Functional Neurology, Rehabilitation, and Ergonomics KW - Neurology KW - Rehabilitation Y1 - 2018 VL - 7 IS - 4 SP - 34 EP - 39 ER - TY - GEN A1 - Salomon, Cornelia A1 - Scherer, Matthias A1 - David, Veronika A1 - Kotzian, Stefan A1 - Forjan, Mathias T1 - Simulation Development of a Virtual Shopping Scenario via HTC VIVE for Neuropsychological Rehabilitation KW - Neurology KW - Rehabilitation KW - Virtual Reality Y1 - 2018 ER - TY - GEN A1 - David, Veronika A1 - Jagos, Harald A1 - Kotzian, Stefan A1 - Forjan, Mathias A1 - Sabo, Anton T1 - Instrumentation of Timed-up-and-Go Test for Stroke Patients using Multisensor Insoles KW - Stroke Patients Y1 - 2018 ER - TY - GEN A1 - Riess, Bernhard A1 - David, Veronika A1 - Scherer, Matthias A1 - Kotzian, Stefan A1 - Forjan, Mathias T1 - Development and Usability Test of an Innovative Low-Cost Rehabilitation Game for the Upper Extremities of Neurological Patients KW - Neurology KW - Health Applications KW - Rehabilitation Y1 - 2018 ER - TY - GEN A1 - Pasteka, Richard A1 - Forjan, Mathias A1 - Drauschke, Andreas T1 - Comparison of breathing patterns for aerosol inhalation using an electro-mechanical lung simulator T2 - ALTEX Proceedings - EUSAAT 2018, Linz KW - Biomedical Engineering KW - mechanical lung-simulator Y1 - 2018 ER - TY - GEN A1 - Gottschalk, Marion A1 - Franzl, Gerald A1 - Frohner, Matthias A1 - Pasteka, Richard A1 - Uslar, Mathias T1 - Structured workflow achieving interoperable smart energy systems KW - Interoperability KW - Energy System KW - Use Case KW - Integration Profiles KW - Testing Y1 - ER - TY - RPRT A1 - Pasteka, Richard T1 - Analysis of influences on inhalable aerosols under characteristic respiratory simulation conditions KW - Mechanical Lung KW - Aerosol KW - Breathing KW - Respiratory system KW - Airflow Y1 - ER - TY - GEN A1 - David, Veronika A1 - Forjan, Mathias A1 - Martinek, Johannes A1 - Kotzian, Stefan A1 - Jagos, Harald A1 - Rafolt, Dietmar T1 - Evaluation of Wearable Multimodal Sensor Insoles for Motion-pattern Measurements in Stroke Rehabilitation – a Pilot Study KW - Stroke Patients KW - Rehabilitation KW - Shoes Y1 - 2018 ER - TY - CHAP A1 - Gottschalk, Marion A1 - Franzl, Gerald A1 - Frohner, Matthias A1 - Paštěka, Richard A1 - Uslar, Mathias T1 - Structured workflow achieving interoperable smart energy systems T2 - Proceedings of the 7th DACH+ Conference on Energy Informatics KW - Interoperability KW - Energy System KW - Use Case KW - Integration Profile KW - Testing Y1 - ER - TY - JOUR A1 - Gottschalk, Marion A1 - Franzl, Gerald A1 - Frohner, Matthias A1 - Paštěka, Richard A1 - Uslar, Mathias T1 - From Integration Profiles to Interoperability Testing for Smart Energy Systems at Connectathon Energy JF - Energies KW - Interoperability KW - Smart Energy System KW - Use Case KW - Testing KW - Connectathon Y1 - IS - 11/12 ER - TY - GEN A1 - Forjan, Mathias A1 - Pasteka, Richard A1 - Drauschke, Andreas T1 - Lung simulation – an alterna� tive approach to animal testing for applications in aerosol and respiratory research T2 - ALTEX Proceedings - EUSAAT 2018, Linz KW - Biomedical Engineering KW - lung simulation Y1 - ER - TY - CHAP A1 - David, Veronika A1 - Forjan, Mathias A1 - Paštěka, Richard A1 - Scherer, Matthias A1 - Hofstätter, Otto T1 - Development of a Multi-Purpose Easy-to-Use Set of Tools for Home Based Rehabilitation T2 - DSAI 2018 - 8th International Conference on Software Development and Technologies for Enhancing Accessibility and Fighting Info-exclusion KW - Rehabitation KW - Low-Cost KW - eHealth KW - Home-based Rehabilitation KW - Telerehabilitation Y1 - ER - TY - CHAP A1 - Pasteka, Richard A1 - Forjan, Mathias A1 - David, Veronika T1 - A Single Point of Contact Data Platform for Rehabilitative Exercises and Equipment T2 - DSAI 2018 - 8th International Conference on Software Development and Technologies for Enhancing Accessibility and Fighting Info-exclusion KW - Telemedicine KW - Rehabilitation KW - Exercise database KW - Home based rehabilitation Y1 - ER - TY - GEN A1 - Scherer, Matthias A1 - Zapf, Stefan A1 - Salomon, Cornelia A1 - Kotzian, Stefan A1 - Forjan, Mathias T1 - Development of a virtual environment for the rehabilitation of neurological patients KW - Virtual Supermarket KW - Neurological Rehabilitation KW - Simulator Sickness KW - Rehabilitation Y1 - ER - TY - CHAP A1 - Oeser, Reinhard A1 - Mense, Alexander A1 - Sauermann, Stefan A1 - Pucher, Robert A1 - Wahl, Harald T1 - Technologies for standards-conform message development: Testing the feasibility of recent CEN prEN 13606 standards developments in Austrian health care T2 - Conference Proceedings 4th International Conference on Information Communication Technologies in Health (ICICTH), Samos Island (Greece) KW - Standards KW - Healthcare Y1 - 2019 SP - 215 EP - 219 ER - TY - CHAP A1 - Mense, Alexander A1 - Kaufmann, Christian A1 - Sauermann, Stefan A1 - Wahl, Harald T1 - Evaluation of methods for securing transmission of medical device or personal health device data from handheld to remote systems T2 - Proceedings of the 8th International Conference on Information and Communication Technology in Health, Samos (Greece) KW - Health data KW - Data Transmission Y1 - 2019 SN - 978-960-466-059-9 ER - TY - CHAP A1 - Pasteka, Richard A1 - Forjan, Mathias A1 - Sauermann, Stefan T1 - Development of a multidisciplinary and telemedicine focused system database T2 - Health Informatics meets eHealth. Digital Insight – Information-Driven Health & Care Proceedings of the 11th eHealth2017 Conference (Studies in Health Technology and Informatics, Volume 236) N2 - Tele-rehabilitation at home is one of the promising approaches in increasing rehabilitative success and simultaneously decreasing the financial burden on the healthcare system. Objectives: Novel and mostly mobile devices are already in use, but shall be used in the future to a higher extent for allowing at home rehabilitation processes at a high quality level. The combination of exercises, assessments and available equipment is the basic objective of the presented database. Methods: The database has been structured in order to allow easy-to-use and fast access for the three main user groups. Therapists – looking for exercise and equipment combinations – patients – rechecking their tasks for home exercises – and manufacturers – entering their equipment for specific use cases. Results: The database has been evaluated by a proof of concept study and shows a high degree of applicability for the field of rehabilitative medicine. Currently it contains 110 exercises/assessments and 111 equipment/systems. Conclusion: Foundations of presented database are already established in the rehabilitative field of application, but can and will be enhanced in its functionality to be usable for a higher variety of medical fields and specificatios. KW - telemedicine KW - e-Health Y1 - SP - 144 EP - 151 PB - IOS Press eBooks ER - TY - CHAP A1 - Frohner, Matthias A1 - Gottschalk, Marion A1 - Franzl, Gerald A1 - Pasteka, Richard A1 - Uslar, Mathias A1 - Sauermann, Stefan T1 - Smart Grid Interoperability Profiles Development T2 - IEEE International Conference on Smart Grid Communications KW - Smart Grid KW - Interoperability KW - Computer Architecture KW - Medical Services KW - Standards Y1 - ER - TY - GEN A1 - Frohner, Matthias A1 - Urbauer, Philipp A1 - Forjan, Mathias A1 - Pohn, Birgit A1 - Gerbovics, Ferenc A1 - Sauermann, Stefan A1 - Mense, Alexander T1 - Development of an Android App in compliance with the Continua Health Alliance Design Guidelines for medical device connectivity in mHealth KW - App development KW - eHealth Y1 - 2019 ER - TY - GEN A1 - Tkachenko Bril, Andres Igor A1 - David, Veronika A1 - Forjan, Mathias A1 - Gaudernak, Jakob A1 - Pils, Katharina T1 - Evaluation of the feasibility of a partial weight-bearing supporting biofeedback system based on instrumented insoles and an Android application KW - Weight-Bearing KW - Insoles KW - Biofeedback System KW - Mobile Application KW - Pilot Study Y1 - ER - TY - CHAP A1 - Hammerl, Barbara A1 - Pucher, Robert A1 - Mense, Alexander A1 - Wahl, Harald A1 - Schmöllebeck, Fritz T1 - Intrinsic motivation of students – the role of proper course design in education for sustainability T2 - Proceedings of the International Conference EMSU2004 Environmental Management for Sustainable Universities, Monterrey (Mexico) KW - Sustainability KW - Motivation KW - Teaching Y1 - 2019 ER - TY - CHAP A1 - Pucher, Robert A1 - Mense, Alexander A1 - Wahl, Harald A1 - Nimmervoll, Alexander A1 - Hofmann, Alexander A1 - Hammerl, Barbara A1 - Schmöllebeck, Fritz T1 - Intrinsic Motivation of Students in PBL Courses T2 - Proceedings of the International Conference PBL2004 Pleasure by Learning, Cancun (Mexico) KW - Problem Based Learning KW - Motivation KW - Teaching Y1 - 2019 ER - TY - GEN A1 - Pasteka, Richard T1 - Applications of Biomedical Engineering in Respiratory Care KW - Biomedical Engineering Y1 - ER - TY - GEN A1 - David, Veronika A1 - Forjan, Mathias A1 - Paštěka, Richard A1 - Scherer, Matthias A1 - Hofstätter, Otto T1 - Development of a Multi-Purpose Easy-to-Use Set of Tools for Home Based Rehabilitation KW - Rehabitation KW - Low-Cost KW - eHealth KW - Home-based Rehabilitation KW - Telerehabilitation Y1 - ER - TY - GEN A1 - Frohner, Matthias A1 - Forjan, Mathias T1 - Interoperability Challenges - How to integrate medical devices KW - Interoperability KW - eHealth KW - Hospital Y1 - ER - TY - JOUR A1 - Urbauer, Philipp A1 - Frohner, Matthias A1 - Forjan, Mathias A1 - Pohn, Birgit A1 - Sauermann, Stefan A1 - Mense, Alexander T1 - A Closer Look on Standards Based Personal Health Device Communication: A Résumé over Four Years Implementing Telemonitoring Solutions JF - European Journal for Biomedical Informatics KW - eHealth KW - Telemonitoring Y1 - 2019 VL - 2012 IS - Vol 8, Issue 3 SP - 65 EP - 70 ER - TY - CHAP A1 - Frohner, Matthias A1 - Urbauer, Philipp A1 - Forjan, Mathias A1 - Pohn, Birgit A1 - Gerbovics, Ferenc A1 - Sauermann, Stefan A1 - Mense, Alexander T1 - Development of an Android App in compliance with the Continua Health Alliance Design Guidelines for medical device connectivity in mHealth T2 - 46th annual conference of the German Society for Biomedical Engineering(BMT 2012) KW - App development KW - eHealth Y1 - 2019 SP - 1051 EP - 1053 ER - TY - CHAP A1 - Pasteka, Richard A1 - Forjan, Mathias T1 - Changes of particle deposition caused by different breathing patterns during active lung simulation T2 - 41st Annual International Conference of the IEEE Engineering in Medicine and Biology Society (EMBC), 2019 KW - Lung Simulation KW - Breathing Simulation KW - Biomedical Engineering Y1 - 2020 SP - 4969 EP - 4972 ER - TY - GEN A1 - David, Veronika A1 - Forjan, Mathias T1 - Rehabilitation at Home - a Device supported Approach KW - Rehabilitation KW - Individualized Rehabilitation KW - Weight bearing KW - Exercise database KW - eHealth Y1 - ER - TY - JOUR A1 - Obergruber, Julian A1 - Mehnen, Lars T1 - Development of a paraglide control system for automatic pitch JF - Proceedings of the 11th Conference of the International Sports Engineering Association (ISEA) 2016 KW - Control System Y1 - 2018 ER - TY - JOUR A1 - Tvorogov, Denis A1 - Anisimov, Andrey A1 - Zheng, Wei A1 - Leppánen, Veli-Matti A1 - Tammela, Tuomas A1 - Laurinavicius, Simonas A1 - Holnthoner, Wolfgang A1 - Helotera, Hanna A1 - Holopainen, Tanja A1 - Jeltsch, Michael A1 - Kalkkinen, Nisse A1 - Lankinen, Hikka A1 - Ojala, Päivi M. A1 - Alitalo, Kari T1 - Effective Suppression of Vascular Network Formation by Combination of Antibodies Blocking VEGFR Ligand Binding and Receptor Dimerization JF - Cancer Cell KW - Vascular Network Y1 - 2019 IS - 18(6) SP - 630 EP - 640 ER - TY - GEN A1 - Mühleder, Severin A1 - Fuchs, Christiane A1 - Bassilio, Jose A1 - Sczwarc, Dorota A1 - Pill, Karoline A1 - Slezak, Paul A1 - Labuda, Krystina A1 - Siehs, Christian A1 - Pröll, Johannes A1 - Priglinger, Eleni A1 - Redl, Heinz A1 - Holnthoner, Wolfgang T1 - The purinergic receptor P2Y2 modulates endothelial sprouting and angiogenesis KW - Angiogenesis Y1 - 2018 ER - TY - JOUR A1 - Simsa, Robin A1 - Padma, Arvind A1 - Heher, Philipp A1 - Hellström, Mats A1 - Teuschl, Andreas A1 - Jenndahl, Lachmi A1 - Bergh, Niklas A1 - Fogelstrand, Per T1 - Systematic in vitro comparison of decellularization protocols for blood vessels. JF - PLoS One KW - Tissue Engineering KW - Decellularization KW - Blood Vessel Y1 - ER - TY - JOUR A1 - Nürnberger, S. A1 - Schneider, C. A1 - Keibl, C. A1 - Schädl, Barbara A1 - Heimel, P. A1 - Monforte, X. A1 - Teuschl, A. H. A1 - Nalbach, M. A1 - Thurner, P. J. A1 - Grillari, J. A1 - Redl, Heinz A1 - Wolbank, S. T1 - Repopulation of decellularised articular cartilage by laser-based matrix engraving JF - EBioMedicine. N2 - Background: In spite of advances in the treatment of cartilage defects using cell and scaffold-based therapeutic strategies, the long-term outcome is still not satisfying since clinical scores decline years after treatment. Scaffold materials currently used in clinical settings have shown limitations in providing suitable biomechanical properties and an authentic and protective environment for regenerative cells. To tackle this problem, we developed a scaffold material based on decellularised human articular cartilage. Methods: Human articular cartilage matrix was engraved using a CO2 laser and treated for decellularisation and glycosaminoglycan removal. Characterisation of the resulting scaffold was performed via mechanical testing, DNA and GAG quantification and in vitro cultivation with adipose-derived stromal cells (ASC). Cell vitality, adhesion and chondrogenic differentiation were assessed. An ectopic, unloaded mouse model was used for the assessment of the in vivo performance of the scaffold in combination with ASC and human as well as bovine chondrocytes. The novel scaffold was compared to a commercial collagen type I/III scaffold. Findings: Crossed line engravings of the matrix allowed for a most regular and ubiquitous distribution of cells and chemical as well as enzymatic matrix treatment was performed to increase cell adhesion. The biomechanical characteristics of this novel scaffold that we term CartiScaff were found to be superior to those of commercially available materials. Neo-tissue was integrated excellently into the scaffold matrix and new collagen fibres were guided by the laser incisions towards a vertical alignment, a typical feature of native cartilage important for nutrition and biomechanics. In an ectopic, unloaded in vivo model, chondrocytes and mesenchymal stromal cells differentiated within the incisions despite the lack of growth factors and load, indicating a strong chondrogenic microenvironment within the scaffold incisions. Cells, most noticeably bone marrow-derived cells, were able to repopulate the empty chondrocyte lacunae inside the scaffold matrix. Interpretation: Due to the better load-bearing, its chondrogenic effect and the ability to guide matrix-deposition, CartiScaff is a promising biomaterial to accelerate rehabilitation and to improve long term clinical success of cartilage defect treatment. Funding: Austrian Research Promotion Agency FFG ("CartiScaff" #842455), Lorenz Böhler Fonds (16/13), City of Vienna Competence Team Project Signaltissue (MA23, #18-08). Keywords: Cartilage regeneration; Decellularisation; Ectopic animal model; Laser engraving; Mechanical testing; Repopulation. KW - Tissue Engineering KW - Cartilage regeneration KW - Mechanical Testing KW - Decellularization KW - Biomaterials Y1 - 2021 VL - 64 IS - 103196. ER - TY - JOUR A1 - Schneider, Karl Heinrich A1 - Enayati, Marjan A1 - Grasl, Christian A1 - Walter, Ingrid A1 - Budinsky, Lubos A1 - Zebic, Gabriel A1 - Kaun, Christoph A1 - Wagner, Anja A1 - Kratochwill, Klaus A1 - Redl, Heinz A1 - Teuschl, Andreas A1 - Podesser, Bruno K. A1 - Bergmeister, Helga T1 - Acellular vascular matrix grafts from human placenta chorion: Impact of ECM preservation on graft characteristics, protein composition and in vivo performance. JF - Biomaterials N2 - Small diameter vascular grafts from human placenta, decellularized with either Triton X-100 (Triton) or SDS and crosslinked with heparin were constructed and characterized. Graft biochemical properties, residual DNA, and protein composition were evaluated to compare the effect of the two detergents on graft matrix composition and structural alterations. Biocompatibility was tested in vitro by culturing the grafts with primary human macrophages and in vivo by subcutaneous implantation of graft conduits (n = 7 per group) into the flanks of nude rats. Subsequently, graft performance was evaluated using an aortic implantation model in Sprague Dawley rats (one month, n = 14). In situ graft imaging was performed using MRI angiography. Retrieved specimens were analyzed by electromyography, scanning electron microscopy, histology and immunohistochemistry to evaluate cell migration and the degree of functional tissue remodeling. Both decellularization methods resulted in grafts of excellent biocompatibility in vitro and in vivo, with low immunogenic potential. Proteomic data revealed removal of cytoplasmic proteins with relative enrichment of ECM proteins in decelluarized specimens of both groups. Noteworthy, LC-Mass Spectrometry analysis revealed that 16 proteins were exclusively preserved in Triton decellularized specimens in comparison to SDS-treated specimens. Aortic grafts showed high patency rates, no signs of thrombus formation, aneurysms or rupture. Conduits of both groups revealed tissue-specific cell migration indicative of functional remodeling. This study strongly suggests that decellularized allogenic grafts from the human placenta have the potential to be used as vascular replacement materials. Both detergents produced grafts with low residual immunogenicity and appropriate mechanical properties. Observed differences in graft characteristics due to preservation method had no impact on successful in vivo performance in the rodent model. KW - Biomaterial KW - Tissue Engineering Y1 - SP - 14 EP - 26 ER - TY - CHAP A1 - Reutterer, Bernd A1 - Traxler, Lukas A1 - Bayer, Natascha A1 - Drauschke, Andreas T1 - Further improvement of an intraocular lens holder for more physiological measurements within a mechanical eye model T2 - Proc.SPIE Biomedical Imaging and Sensing Conference KW - Mechanical Eye Y1 - 2018 ER - TY - GEN A1 - Traxler, Lukas A1 - Bayer, Natascha A1 - Reutterer, Bernd A1 - Husinsky, Wolfgang A1 - Drauschke, Andreas T1 - Prüfung der optischen Qualität von Intraokularlinsen auf Tolerant gegenüber Fehlplatzierungen im Auge KW - Eye Lens KW - Mechanical Eye Y1 - 2018 ER - TY - GEN A1 - Traxler, Lukas A1 - Bayer, Natascha A1 - Reutterer, Bernd A1 - Drauschke, Andreas T1 - Improvement of Optics, Mechanics and the Usability of a Mechanical Eye Model for Vision Quality Evaluation of IOLs KW - Mechanical Eye Y1 - ER - TY - JOUR A1 - Teuschl, Andreas A1 - Holnthoner, Wolfgang A1 - Monforte, Xavier T1 - Repopulation of an auricular cartilage scaffold, AuriScaff, perforated with an enzyme combination JF - Acta Biomater. N2 - Biomaterials currently in use for articular cartilage regeneration do not mimic the composition or architecture of hyaline cartilage, leading to the formation of repair tissue with inferior characteristics. In this study we demonstrate the use of "AuriScaff", an enzymatically perforated bovine auricular cartilage scaffold, as a novel biomaterial for repopulation with regenerative cells and for the formation of high-quality hyaline cartilage. AuriScaff features a traversing channel network, generated by selective depletion of elastic fibers, enabling uniform repopulation with therapeutic cells. The complex collagen type II matrix is left intact, as observed by immunohistochemistry, SEM and TEM. The compressive modulus is diminished, but three times higher than in the clinically used collagen type I/III scaffold that served as control. Seeding tests with human articular chondrocytes (hAC) alone and in co-culture with human adipose-derived stromal/stem cells (ASC) confirmed that the network enabled cell migration throughout the scaffold. It also guides collagen alignment along the channels and, due to the generally traverse channel alignment, newly deposited cartilage matrix corresponds with the orientation of collagen within articular cartilage. In an osteochondral plug model, AuriScaff filled the complete defect with compact collagen type II matrix and enabled chondrogenic differentiation inside the channels. Using adult articular chondrocytes from bovine origin (bAC), filling of even deep defects with high-quality hyaline-like cartilage was achieved after 6 weeks in vivo. With its composition and spatial organization, AuriScaff provides an optimal chondrogenic environment for therapeutic cells to treat cartilage defects and is expected to improve long-term outcome by channel-guided repopulation followed by matrix deposition and alignment. STATEMENT OF SIGNIFICANCE: After two decades of tissue engineering for cartilage regeneration, there is still no optimal strategy available to overcome problems such as inconsistent clinical outcome, early and late graft failures. Especially large defects are dependent on biomaterials and their scaffolding, guiding and protective function. Considering the currently used biomaterials, structure and mechanical properties appear to be insufficient to fulfill this task. The novel scaffold developed within this study is the first approach enabling the use of dense cartilage matrix, repopulate it via channels and provide the cells with a compact collagen type II environment. Due to its density, it also provides better mechanical properties than materials currently used in clinics. We therefore think, that the auricular cartilage scaffold (AuriScaff) has a high potential to improve future cartilage regeneration approaches. KW - Auricular cartilage KW - Cartilage Regeneration KW - Human adipose derived stromal/stem cells KW - Tissue Engineering KW - Decellularization Y1 - 2020 VL - 2019 IS - Mar/86 SP - 207 EP - 222 ER - TY - GEN A1 - Rank, Elisabet A1 - Traxler, Lukas A1 - Bayer, Natascha A1 - Reutterer, Bernd A1 - Lux, Kirsten A1 - Drauschke, Andreas T1 - Reproducibility analysis of measurements with a mechanical semiautomatic eye model for evaluation of intraocular lenses KW - Mechanical Eye Y1 - ER - TY - GEN A1 - Obergruber, Julian A1 - Mehnen, Lars T1 - Development of a paraglide control system for automatic pitch stabilization to increase the passive safety KW - Paragliding KW - Safety Y1 - 2018 ER - TY - THES A1 - Traxler, Lukas T1 - Testing the Optical Quality of Intraocular Lenses Regarding Postoperative Tilt and Decentration KW - Intraocular Lens KW - Cataract KW - Testing method Y1 - PB - TU Wien - Fakultät für Physik CY - Wien ER - TY - GEN A1 - Traxler, Lukas A1 - Reutterer, Bernd A1 - Bayer, Natascha A1 - Rank, Elisabet A1 - Krause, Sylvio A1 - Beckert, Erik A1 - Drauschke, Andreas T1 - Tilt and decentration tolerance of intraocular lenses: measurements with an improved mechanical model eye KW - Eye Lens KW - Mechanical Eye Y1 - 2018 ER - TY - JOUR A1 - Priglinger, Eleni A1 - Schuh, Christina A1 - Steffenhagen, Carolin A1 - Wurzer, Christoph A1 - Maier, Julia A1 - Nürnberger, Sylvia A1 - Holnthoner, Wolfgang A1 - Fuchs, Christiane A1 - Suessner, Susanne A1 - Rünzler, Dominik A1 - Redl, Heinz A1 - Wolbank, Susanne T1 - Improvement of adipose tissue-derived cells by low-energy extracorporeal shock wave therapy. JF - Cytotherapy N2 - BACKGROUND: Cell-based therapies with autologous adipose tissue-derived cells have shown great potential in several clinical studies in the last decades. The majority of these studies have been using the stromal vascular fraction (SVF), a heterogeneous mixture of fibroblasts, lymphocytes, monocytes/macrophages, endothelial cells, endothelial progenitor cells, pericytes and adipose-derived stromal/stem cells (ASC) among others. Although possible clinical applications of autologous adipose tissue-derived cells are manifold, they are limited by insufficient uniformity in cell identity and regenerative potency. METHODS: In our experimental set-up, low-energy extracorporeal shock wave therapy (ESWT) was performed on freshly obtained human adipose tissue and isolated adipose tissue SVF cells aiming to equalize and enhance stem cell properties and functionality. RESULTS: After ESWT on adipose tissue we could achieve higher cellular adenosine triphosphate (ATP) levels compared with ESWT on the isolated SVF as well as the control. ESWT on adipose tissue resulted in a significantly higher expression of single mesenchymal and vascular marker compared with untreated control. Analysis of SVF protein secretome revealed a significant enhancement in insulin-like growth factor (IGF)-1 and placental growth factor (PLGF) after ESWT on adipose tissue. DISCUSSION: Summarizing we could show that ESWT on adipose tissue enhanced the cellular ATP content and modified the expression of single mesenchymal and vascular marker, and thus potentially provides a more regenerative cell population. Because the effectiveness of autologous cell therapy is dependent on the therapeutic potency of the patient's cells, this technology might raise the number of patients eligible for autologous cell transplantation. KW - Shockwave Therapy KW - Tissue Regeneration KW - Regenerative Medicine Y1 - SP - 1079 EP - 1095 ER - TY - JOUR A1 - Bachmann, Barbara A1 - Spitz, Sarah A1 - Rothbauer, Mario A1 - Jordan, Christian A1 - Purtscher, Michaela A1 - Zirath, Helene A1 - Schuller, Patrick A1 - Eilenberger, Christoph A1 - Ali, Syed Faheem A1 - Mühleder, Severin A1 - Priglinger, Eleni A1 - Harasek, Michael A1 - Redl, Heinz A1 - Holnthoner, Wolfgang A1 - Ertl, Peter T1 - Engineering of three-dimensional pre-vascular networks within fibrin hydrogel constructs by microfluidic control over reciprocal cell signaling JF - Biomicrofluidics KW - Microfluidic KW - Vascularization KW - Tissue Engineering Y1 - 2019 ER - TY - GEN A1 - Eckelt, Markus A1 - Herczeg, C. A1 - Sabo, Anton T1 - Gesundheitsfördernde Maßnahmen am Arbeitsplatz durch Nordic Walking KW - Sport KW - Nordic Walking Y1 - 2019 ER - TY - JOUR A1 - Cieślik-Boczula, Katarzyna A1 - Küpcü, Seta A1 - Rünzler, Dominik A1 - Koll, Aleksander A1 - Köhler, Gottfried T1 - Effects of the phenolic lipid 3-pentadecylphenol on phospholipid bilayer organization JF - Journal of Molecular Structure KW - Phospholipid Bilayer Y1 - 2019 IS - 919(1-3) SP - 373 EP - 380 ER - TY - GEN A1 - Schneider, Karl Heinrich A1 - Aigner, Petra A1 - Monforte Vila, Xavier A1 - Holnthoner, Wolfgang A1 - Teuschl, Andreas A1 - Bergmeister, Helga A1 - Redl, Heinz T1 - Naturally derived acellular small diameter vascular grafts from human placenta for reconstructive surgery KW - Placenta KW - Grafting KW - Surgery Y1 - 2018 ER - TY - GEN A1 - Nemec, Iris T1 - Current prosthetic projects and prosthetic developments KW - Prosthetics KW - 3D Printing KW - Use Case Y1 - ER - TY - JOUR A1 - Schneider, Karl A1 - Rohringer, Sabrina A1 - Kapeller, Barbara A1 - Grasl, Christian A1 - Kiss, Herbert A1 - Heber, Stefan A1 - Walter, Ingrid A1 - Teuschl, Andreas A1 - Podesser, Bruno K. A1 - Bergmeister, Helga T1 - Riboflavin-mediated photooxidation to improve the characteristics of decellularized human arterial small diameter vascular grafts JF - Acta Biomater. N2 - Vascular grafts with a diameter of less than 6 mm are made from a variety of materials and techniques to provide alternatives to autologous vascular grafts. Decellularized materials have been proposed as a possible approach to create extracellular matrix (ECM) vascular prostheses as they are naturally derived and inherently support various cell functions. However, these desirable graft characteristics may be limited by alterations of the ECM during the decellularization process leading to decreased biomechanical properties and hemocompatibility. In this study, arteries from the human placenta chorion were decellularized using two distinct detergents (Triton X-100 or SDS), which differently affect ECM ultrastructure. To overcome biomechanical strength loss and collagen fiber exposure after decellularization, riboflavin-mediated UV (RUV) crosslinking was used to uniformly crosslink the collagenous ECM of the grafts. Graft characteristics and biocompatibility with and without RUV crosslinking were studied in vitro and in vivo. RUV-crosslinked ECM grafts showed significantly improved mechanical strength and smoothening of the luminal graft surfaces. Cell seeding using human endothelial cells revealed no cytotoxic effects of the RUV treatment. Short-term aortic implants in rats showed cell migration and differentiation of host cells. Functional graft remodeling was evident in all grafts. Thus, RUV crosslinking is a preferable tool to improve graft characteristics of decellularized matrix conduits. KW - Tissue Engineering KW - Biomaterials KW - Vascularization Y1 - 2021 VL - 2020 IS - 116 SP - 246 EP - 258 ER - TY - CHAP A1 - Reutterer, Bernd A1 - Traxler, Lukas A1 - Bayer, Natascha A1 - Drauschke, Andreas T1 - Comparison of different wavefront measurement setups to judge the position tolerance of intraocular lenses in a model eye T2 - Proc.SPIE Biomedical Imaging and Sensing Conference KW - Mechanical Eye KW - Eye Lens Y1 - 2018 ER - TY - JOUR A1 - Schneider, Jaana A1 - Pultar, Marianne A1 - Oesterreicher, Johannes A1 - Bobbili, Madhusudhan Reddy A1 - Mühleder, Severin A1 - Priglinger, Eleni A1 - Redl, Heinz A1 - Spittler, Andreas A1 - Grillari, Johannes A1 - Holnthoner, Wolfgang T1 - Cre mRNA Is Not Transferred by EVs from Endothelial and Adipose-Derived Stromal/Stem Cells during Vascular Network Formation JF - Int J Mol Sci. N2 - Coculture systems employing adipose tissue-derived mesenchymal stromal/stem cells (ASC) and endothelial cells (EC) represent a widely used technique to model vascularization. Within this system, cell-cell communication is crucial for the achievement of functional vascular network formation. Extracellular vesicles (EVs) have recently emerged as key players in cell communication by transferring bioactive molecules between cells. In this study we aimed to address the role of EVs in ASC/EC cocultures by discriminating between cells, which have received functional EV cargo from cells that have not. Therefore, we employed the Cre-loxP system, which is based on donor cells expressing the Cre recombinase, whose mRNA was previously shown to be packaged into EVs and reporter cells containing a construct of floxed dsRed upstream of the eGFP coding sequence. The evaluation of Cre induced color switch in the reporter system via EVs indicated that there is no EV-mediated RNA transmission either between EC themselves or EC and ASC. However, since Cre mRNA was not found present in EVs, it remains unclear if Cre mRNA is generally not packaged into EVs or if EVs are not taken up by the utilized cell types. Our data indicate that this technique may not be applicable to evaluate EV-mediated cell-to-cell communication in an in vitro setting using EC and ASC. Further investigations will require a functional system showing efficient and specific loading of Cre mRNA or protein into EVs. KW - Tissue Engineering KW - Stem Cells KW - Vascular Network Formation KW - EVs KW - Endothelial Cells Y1 - VL - 2021 IS - 22(8) SP - 4050 ER - TY - JOUR A1 - Nürnberger, Sylvia A1 - Schneider, Cornelia A1 - van Osch, Gerjo A1 - Keibl, Claudia A1 - Rieder, Bernhard A1 - Monforte, Xavier A1 - Teuschl, Andreas A1 - Mühleder, Severin A1 - Holnthoner, Wolfgang A1 - Schädl, Barbara A1 - Gahleitner, Christoph A1 - Redl, Heinz A1 - Wolbank, Susanne T1 - Repopulation of an auricular cartilage scaffold, AuriScaff, perforated with an enzyme combination. JF - Acta Biomaterialia KW - Tissue Engineering KW - Decellularization KW - Cartilage Y1 - ER - TY - CHAP A1 - Traxler, Lukas A1 - Reutterer, Bernd A1 - Bayer, Natascha A1 - Rank, Elisabet A1 - Krause, Sylvio A1 - Beckert, Erik A1 - Drauschke, Andreas T1 - Tilt and decentration tolerance of intraocular lenses: measurements with an improved mechanical model eye T2 - Proceedings of the 26th Conference on Ophtalmic Technologies 2016 KW - Eye Lens KW - Mechanical Eye Y1 - 2018 ER - TY - CHAP A1 - Traxler, Lukas A1 - Bayer, Natascha A1 - Reutterer, Bernd A1 - Drauschke, Andreas T1 - Improvement of Optics, Mechanics and the Usability of a Mechanical Eye Model for Vision Quality Evaluation of IOLs T2 - Proceedings of the 13th IFAC and IEEE Conference on Programmable Devices and Embedded Systems (PDES) 2015 KW - Mechanical Eye Y1 - ER - TY - JOUR A1 - Johannes, Hackethal A1 - Weihs, Anna A1 - Karner, Lisa A1 - Metzger, Magdalena A1 - Dungel, Peter A1 - Hennerbichler, Simone A1 - Redl, Heinz A1 - Teuschl-Woller, Andreas Herbert T1 - Novel Human Placenta-Based Extract for Vascularization Strategies in Tissue Engineering JF - Tissue Eng Part C Methods N2 - There is critical unmet need for new vascularized tissues to support or replace injured tissues and organs. Various synthetic and natural materials were already established for use of two-dimensional (2D) and three-dimensional (3D) in vitro neovascularization assays, however, they still cannot mimic the complex functions of the sum of the extracellular matrix (ECM) in native intact tissue. Currently, this issue is only addressed by artificial products such as Matrigel™, which comprises a complex mixture of ECM proteins, extracted from animal tumor tissue. Despite its outstanding bioactivity, the isolation from tumor tissue hinders its translation into clinical applications. Since nonhuman ECM proteins may cause immune reactions, as are frequently observed in clinical trials, human ECM proteins represent the best option when aiming for clinical applications. Here, we describe an effective method of isolating a human placenta substrate (hpS) that induces the spontaneous formation of an interconnected network of green fluorescence-labeled human umbilical vein endothelial cells (gfpHUVECs) in vitro. The substrate was biochemically characterized by using a combination of bicinchoninic acid (BCA) assay, DNA, and glycosaminoglycan (GAG) content assays, sodium dodecyl sulfate/polyacrylamide gel electrophoresis (SDS-PAGE) analysis and Western blot, angiogenesis arrays, chromatographic thrombin detection, high performance liquid chromatography (HPLC)-based amino acid quantification analysis, and assessment of antimicrobial properties. 2D in vitro cell culture experiments have been performed to determine the vasculogenic potential of hpS, which demonstrated that cell networks developed on hpS show a significantly higher degree of complexity (number of tubules/junctions; total/mean tube length) when compared with Matrigel. As 3D cell culture techniques represent a more accurate representation of the in vivo condition, the substrate was 3D solidified using various natural polymers. 3D in vitro vasculogenesis assays have been performed by seeding gfpHUVECs in an hpS-fibrinogen clot. In conclusion, hpS provides a potent human/material-based alternative to xenogenic-material-based biomaterials for vascularization strategies in tissue engineering. KW - Tissue Engineering KW - Biomaterials KW - HUVEC KW - Acellular biological matrices KW - Angiogenesis and vasculogenesis Y1 - VL - 27 IS - 11 SP - 616 EP - 632 ER - TY - CHAP A1 - Hörzer, S. A1 - Fuchs, Christiane A1 - Gastinger, R. A1 - Sabo, Anton A1 - Mehnen, Lars A1 - Martinek, Johannes A1 - Reichel, Martin T1 - Simulation of spinning soccer ball trajectories influenced by altitude T2 - Procedia Engineering KW - Soccer Y1 - 2019 SP - 2461 EP - 2466 ER - TY - GEN A1 - Reutterer, Bernd A1 - Traxler, Lukas A1 - Bayer, Natascha A1 - Drauschke, Andreas T1 - Further improvement of an intraocular lens holder for more physiological measurements within a mechanical eye model KW - Mechanical Eye Y1 - 2018 ER - TY - CHAP A1 - Reutterer, Bernd A1 - Traxler, Lukas A1 - Bayer, Natascha A1 - Drauschke, Andreas T1 - Experiments for practical education in process parameter optimization for selective laser sintering to increase workpiece quality T2 - Proceedings of the 3rd Conference on Laser 3D Manufacturing 2016 KW - Laser Sintering Y1 - 2018 ER - TY - CHAP A1 - Knebl, Gerald A1 - Morton, Tatjana J. A1 - Redl, Heinz A1 - Rünzler, Dominik T1 - Mechanical stimulation of cells in 3-dimensional fibrin constructs using a bioreactor T2 - 3. Forschungsforum der österreichischen Fachhochschulen / Fachhochschule Kärnten KW - Cells KW - Bioreactor Y1 - 2019 SP - 492 EP - 493 ER - TY - CHAP A1 - Mense, Alexander A1 - Frohner, Matthias A1 - Kaufmann, Christian A1 - Pohn, Birgit A1 - Sauermann, Stefan A1 - Urbauer, Philipp A1 - Wahl, Harald T1 - Federated Identity Management for management and integration of personal health data archives T2 - PROCEEDINGS of the 10th International Conference on INFORMATION COMMUNICATION TECHNOLOGIES IN HEALTH, Chrysovaladis Prachalias (ed.), National and Kapodistrian University of Athens KW - Identity Management KW - Personal Health Y1 - 2019 ER - TY - JOUR A1 - Traxler, Lukas A1 - Bayer, Natascha A1 - Reutterer, Bernd A1 - Drauschke, Andreas A1 - Husinsky, Wolfgang T1 - Prüfung der optischen Qualität von Intraokularlinsen auf Tolerant gegenüber Fehlplatzierungen im Auge JF - Proceedings der Jahrestagung der Österreichischen Gesellschaft für Biomedizinische Technik (ÖGBMT) 2015 KW - Eye Lens KW - Mechanical Eye KW - Measurement Error Y1 - 2018 ER - TY - JOUR A1 - Schneider, Karl H. A1 - Rohringer, Sabrina A1 - Kapeller, Barbara A1 - Grasl, Christian A1 - Kiss, Herbert A1 - Heber, Stefan A1 - Walter, Ingrid A1 - Teuschl, Andreas H. A1 - Podesser, Bruno K. A1 - Bergmeister, Helga T1 - Riboflavin-mediated photooxidation to improve the characteristics of decellularized human arterial small diameter vascular grafts JF - Acta Biomaterialia N2 - Vascular grafts with a diameter of less than 6 mm are made from a variety of materials and techniques to provide alternatives to autologous vascular grafts. Decellularized materials have been proposed as a possible approach to create extracellular matrix (ECM) vascular prostheses as they are naturally derived and inherently support various cell functions. However, these desirable graft characteristics may be limited by alterations of the ECM during the decellularization process leading to decreased biomechanical properties and hemocompatibility. In this study, arteries from the human placenta chorion were decellularized using two distinct detergents (Triton X-100 or SDS), which differently affect ECM ultrastructure. To overcome biomechanical strength loss and collagen fiber exposure after decellularization, riboflavin-mediated UV (RUV) crosslinking was used to uniformly crosslink the collagenous ECM of the grafts. Graft characteristics and biocompatibility with and without RUV crosslinking were studied in vitro and in vivo. RUV-crosslinked ECM grafts showed significantly improved mechanical strength and smoothening of the luminal graft surfaces. Cell seeding using human endothelial cells revealed no cytotoxic effects of the RUV treatment. Short-term aortic implants in rats showed cell migration and differentiation of host cells. Functional graft remodeling was evident in all grafts. Thus, RUV crosslinking is a preferable tool to improve graft characteristics of decellularized matrix conduits. KW - Tissue Engineering KW - Small diameter vascular graft KW - Biomaterials KW - Decellularization KW - Surface modification Y1 - VL - 116 SP - 246 EP - 258 ER - TY - JOUR A1 - Heinzel, Johannes Christoph A1 - Oberhauser, Viola A1 - Keibl, Claudia A1 - Schädl, Barbara A1 - Swiadek, Nicole V. A1 - Längle, Gregor A1 - Frick, Helen A1 - Slezak, Cyrill A1 - Prahm, Cosima A1 - Grillari, Johannes A1 - Kolbenschlag, Jonas A1 - Hercher, David T1 - ESWT Diminishes Axonal Regeneration following Repair of the Rat Median Nerve with Muscle-In-Vein Conduits but Not after Autologous Nerve Grafting JF - Biomedicines N2 - Investigations reporting positive effects of extracorporeal shockwave therapy (ESWT) on nerve regeneration are limited to the rat sciatic nerve model. The effects of ESWT on muscle-in-vein conduits (MVCs) have also not been investigated yet. This study aimed to evaluate the effects of ESWT after repair of the rat median nerve with either autografts (ANGs) or MVCs. In male Lewis rats, a 7 mm segment of the right median nerve was reconstructed either with an ANG or an MVC. For each reconstructive technique, one group of animals received one application of ESWT while the other rats served as controls. The animals were observed for 12 weeks, and nerve regeneration was assessed using computerized gait analysis, the grasping test, electrophysiological evaluations and histological quantification of axons, blood vessels and lymphatic vasculature. Here, we provide for the first time a comprehensive analysis of ESWT effects on nerve regeneration in a rat model of median nerve injury. Furthermore, this study is among the first reporting the quantification of lymphatic vessels following peripheral nerve injury and reconstruction in vivo. While we found no significant direct positive effects of ESWT on peripheral nerve regeneration, results following nerve repair with MVCs were significantly inferior to those after ANG repair. KW - Tissue Engineering KW - Muscle-In-Vein Conduits KW - Axonal Regeneration KW - Autologous Nerve Grafting Y1 - VL - 2022 IS - 10(8) SP - 1777 ER - TY - JOUR A1 - Verstraeten, Valerie A1 - Holnthoner, Wolfgang A1 - Van Steensel, Maurice A1 - Veraart, Joep C J M A1 - Bladergroen, Reno S A1 - Heckmann, Caronline A A1 - Keskitalo, Salla A1 - Frank, Jorge A1 - Alitalo, Kari A1 - van Geel, Michel A1 - Steijlen, Peter M T1 - Functional analysis of FLT4 mutations associated with Nonne-Milroy lymphedema JF - Journal of Investigative Dermatology KW - Nonne-Milroy iymphedema KW - Mutation Y1 - 2019 IS - 129(2) SP - 509 EP - 512 ER - TY - JOUR A1 - Holnthoner, Wolfgang T1 - Adipose-tissue-derived therapeutic cells in their natural environment as an autologous cell therapy strategy: the microtissue-stromal vascular fraction JF - Eur Cell Mater. N2 - The prerequisite for a successful clinical use of autologous adipose-tissue-derived cells is the highest possible regenerative potential of the applied cell population, the stromal vascular fraction (SVF). Current isolation methods depend on high enzyme concentration, lysis buffer, long incubation steps and mechanical stress, resulting in single cell dissociation. The aim of the study was to limit cell manipulation and obtain a derivative comprising therapeutic cells (microtissue-SVF) without dissociation from their natural extracellular matrix, by employing a gentle good manufacturing practice (GMP)-grade isolation. The microtissue-SVF yielded larger numbers of viable cells as compared to the improved standard-SVF, both with low enzyme concentration and minimal dead cell content. It comprised stromal tissue compounds (collagen, glycosaminoglycans, fibroblasts), capillaries and vessel structures (CD31+, smooth muscle actin+). A broad range of cell types was identified by surface-marker characterisation, including mesenchymal, haematopoietic, pericytic, blood and lymphatic vascular and epithelial cells. Subpopulations such as supra-adventitial adipose-derived stromal/stem cells and endothelial progenitor cells were significantly more abundant in the microtissue-SVF, corroborated by significantly higher potency for angiogenic tube-like structure formation in vitro. The microtissue-SVF showed the characteristic phenotype and tri-lineage mesenchymal differentiation potential in vitro and an immunomodulatory and pro-angiogenic secretome. In vivo implantation of the microtissue-SVF combined with fat demonstrated successful graft integration in nude mice. The present study demonstrated a fast and gentle isolation by minor manipulation of liposuction material, achieving a therapeutically relevant cell population with high vascularisation potential and immunomodulatory properties still embedded in a fraction of its original matrix. KW - Adipose-tissue KW - autologous cell therapy KW - microtissue KW - stromal vascular fraction KW - vascularization Y1 - 2020 VL - 2019 IS - Feb, 37 SP - 113 EP - 133 ER - TY - JOUR A1 - Schneider, Karl Heinrich A1 - Aigner, Petra A1 - Holnthoner, Wolfgang A1 - Monforte Vila, Xavier A1 - Nürnberger, Sylvia A1 - Rünzler, Dominik A1 - Redl, Heinz A1 - Teuschl, Andreas T1 - Decellularized human placenta chorion matrix as a favorable source of small-diameter vascular grafts JF - Acta Biomaterialia KW - Grafting KW - Tissue Engineering Y1 - 2018 ER - TY - JOUR A1 - Heimel, Patrick A1 - Swiadek, Nicole V. A1 - Slezak, Paul A1 - Kerbl, Markus A1 - Schneider, Cornelia A1 - Nürnberger, Sylvia A1 - Redl, Heinz A1 - Teuschl, Andreas A1 - Hercher, David T1 - Iodine-Enhanced Micro-CT Imaging of Soft Tissue on the Example of Peripheral Nerve Regeneration JF - Contrast Media & Molecular Imaging KW - µCT KW - Imaging KW - Tissue Engineering KW - Tissue Regeneration Y1 - ER - TY - GEN A1 - Reutterer, Bernd A1 - Traxler, Lukas A1 - Bayer, Natascha A1 - Drauschke, Andreas T1 - Experiments for practical education in process parameter optimization for selective laser sintering to increase workpiece quality KW - Laser Sintering KW - Education Y1 - 2018 ER - TY - JOUR A1 - Strohmeier, Karin A1 - Hofmann, Martina A1 - Jacak, Jaroslaw A1 - Narzt, Marie-Sophie A1 - Wahlmueller, Marlene A1 - Mairhofer, Mario A1 - Schädl, Barbara A1 - Holnthoner, Wolfgang A1 - Barsch, Martin A1 - Sandhofer, Matthias A1 - Wolbank, Susanne A1 - Priglinger, Eleni T1 - Multi-Level Analysis of Adipose Tissue Reveals the Relevance of Perivascular Subpopulations and an Increased Endothelial Permeability in Early-Stage Lipedema JF - Biomedicines N2 - Lipedema is a chronic, progressive disease of adipose tissue with unknown etiology. Based on the relevance of the stromal vascular fraction (SVF) cell population in lipedema, we performed a thorough characterization of subcutaneous adipose tissue, SVF isolated thereof and the sorted populations of endothelial cells (EC), pericytes and cultured adipose-derived stromal/stem cells (ASC) of early-stage lipedema patients. We employed histological and gene expression analysis and investigated the endothelial barrier by immunofluorescence and analysis of endothelial permeability in vitro. Although there were no significant differences in histological stainings, we found altered gene expression of factors relevant for local estrogen metabolism (aromatase), preadipocyte commitment (ZNF423) and immune cell infiltration (CD11c) in lipedema on the tissue level, as well as in distinct cellular subpopulations. Machine learning analysis of immunofluorescence images of CD31 and ZO-1 revealed a morphological difference in the cellular junctions of EC cultures derived from healthy and lipedema individuals. Furthermore, the secretome of lipedema-derived SVF cells was sufficient to significantly increase leakiness of healthy human primary EC, which was also reflected by decreased mRNA expression of VE-cadherin. Here, we showed for the first time that the secretome of SVF cells creates an environment that triggers endothelial barrier dysfunction in early-stage lipedema. Moreover, since alterations in gene expression were detected on the cellular and/or tissue level, the choice of sample material is of high importance in elucidating this complex disease. KW - Tissue Engineering KW - Adipose Tissue KW - Lipedema KW - Endothelial Cells Y1 - VL - 2022 IS - 10(5) SP - 1163 ER - TY - JOUR A1 - Rohringer, Sabrina A1 - Holnthoner, Wolfgang A1 - Hackl, Matthias A1 - Weihs, Anna A1 - Rünzler, Dominik A1 - Skalicky, Susanna A1 - Karbiener, Michael A1 - Scheideler, Marcel A1 - Pröll, Johannes A1 - Gabriel, Christian A1 - Schweighofer, Bernhard A1 - Gröger, Marion A1 - Spittler, Andreas A1 - Grillari, Johannes A1 - Redl, Heinz T1 - Molecular and cellular effects of in vitro shockwave treatment on lymphatic endothelial cells. JF - PLoS one KW - Shockwave Y1 - ER - TY - CHAP A1 - Bayer, Natascha A1 - Rank, Elisabet A1 - Traxler, Lukas A1 - Beckert, Erik A1 - Drauschke, Andreas T1 - Implementation of a capsular bag model to enable sufficient lens stabilization within a mechanical eye model T2 - Proc.SPIE 9307, Ophthalmic Technologies XXV KW - Mechanical Eye Y1 - ER - TY - JOUR A1 - Holnthoner, Wolfgang A1 - Szwarc, Dorota T1 - Purinergic P2Y 2 receptors modulate endothelial sprouting JF - Cell Mol Life Sci. N2 - Purinergic P2 receptors are critical regulators of several functions within the vascular system, including platelet aggregation, vascular inflammation, and vascular tone. However, a role for ATP release and P2Y receptor signalling in angiogenesis remains poorly defined. Here, we demonstrate that blood vessel growth is controlled by P2Y2 receptors. Endothelial sprouting and vascular tube formation were significantly dependent on P2Y2 expression and inhibition of P2Y2 using a selective antagonist blocked microvascular network generation. Mechanistically, overexpression of P2Y2 in endothelial cells induced the expression of the proangiogenic molecules CXCR4, CD34, and angiopoietin-2, while expression of VEGFR-2 was decreased. Interestingly, elevated P2Y2 expression caused constitutive phosphorylation of ERK1/2 and VEGFR-2. However, stimulation of cells with the P2Y2 agonist UTP did not influence sprouting unless P2Y2 was constitutively expressed. Finally, inhibition of VEGFR-2 impaired spontaneous vascular network formation induced by P2Y2 overexpression. Our data suggest that P2Y2 receptors have an essential function in angiogenesis, and that P2Y2 receptors present a therapeutic target to regulate blood vessel growth. KW - Angiogenesis KW - Endothelial KW - Purinergic KW - P2Y2 KW - Cell Sprouting Y1 - 2020 VL - 2020 IS - Mar. SP - 885 EP - 901 ER - TY - GEN A1 - Gerbovics, Ferenc A1 - Haller, Michael A1 - Traxler, Lukas A1 - Urbauer, Philipp A1 - Frohner, Matthias A1 - Sauermann, Stefan A1 - Mense, Alexander T1 - Implementation of a wrist wearable Health Data Hub-prototype for context based telemonitoring of elderly people using standardized data transfer methods KW - Health data KW - Telemonitoring Y1 - 2019 SN - 978-1-60750-658-4 SN - 0926-9630 ER - TY - CHAP A1 - Rank, Elisabet A1 - Traxler, Lukas A1 - Bayer, Natascha A1 - Reutterer, Bernd A1 - Lux, Kirsten A1 - Drauschke, Andreas T1 - Reproducibility analysis of measurements with a mechanical semiautomatic eye model for evaluation of intraocular lenses T2 - Proc.SPIE 8936, Design and Quality for Biomedical Technologies VII KW - Mechanical Eye Y1 - ER - TY - GEN A1 - Traxler, Lukas T1 - Klare Sicht nach Grauem Star KW - Intraocular Lenses KW - Cataract KW - Testing method Y1 - ER - TY - JOUR A1 - Trisko, Johanna A1 - Fleck, Johanna A1 - Kau, Silvio A1 - Oesterreicher, Johannes A1 - Holnthoner, Wolfgang T1 - Lymphatic and Blood Endothelial Extracellular Vesicles: A Story Yet to Be Written JF - Life N2 - Extracellular vesicles (EVs), such as exosomes, microvesicles, and apoptotic bodies, are cell-derived, lipid bilayer-enclosed particles mediating intercellular communication and are therefore vital for transmitting a plethora of biological signals. The vascular endothelium substantially contributes to the circulating particulate secretome, targeting important signaling pathways that affect blood cells and regulate adaptation and plasticity of endothelial cells in a paracrine manner. Different molecular signatures and functional properties of endothelial cells reflect their heterogeneity among different vascular beds and drive current research to understand varying physiological and pathological effects of blood and lymphatic endothelial EVs. Endothelial EVs have been linked to the development and progression of various vascular diseases, thus having the potential to serve as biomarkers and clinical treatment targets. This review aims to provide a brief overview of the human vasculature, the biology of extracellular vesicles, and the current knowledge of endothelium-derived EVs, including their potential role as biomarkers in disease development. KW - Tissue Engineering KW - Endothelial Cells KW - vascularization KW - extracellular vesicles KW - lymphatic endothelial cells Y1 - VL - 2022 IS - 12(5) SP - 654 ER - TY - JOUR A1 - Ferner-Ortner-Bleckmann, Judith A1 - Huber-Gries, Carina A1 - Pavkov-Keller, Tea A1 - Keller, Walter A1 - Mader, Christoph A1 - Ilk, Nicola A1 - Sleytr, Uwe B. A1 - Egelseer, Eva-Maria T1 - The high-molecular-mass amylase (HMMA) of Geobacillus stearothermophilus ATCC 12980 interacts with the cell wall components by virtue of three specific binding regions JF - Molecular Microbiology KW - Cells Y1 - 2019 IS - 72(6) SP - 1448 EP - 1461 ER - TY - CHAP A1 - Pfützner, Helmut A1 - Kaniusas, Eugenijus A1 - Kosel, Jürgen A1 - Mehnen, Lars A1 - Meydan, Turgut A1 - Borza, Firuta A1 - Vazquez, Manuel A1 - Rohn, Michael A1 - Marquardt, Bernd T1 - First Magnetic Materials with Sensitivity for the Physical Quantity of 'Curvature' T2 - 4th Japanese Mediterranean Workshop on Applied Electromagnetic Engineering for Magnetic, Superconducting and Nano Materials KW - Magnetic KW - Materials Y1 - 2019 SP - 177 EP - 178 ER - TY - GEN A1 - Liousia, Varvara A1 - Salzer, Elias A1 - Mandt, Denise A1 - Rünzler, Dominik T1 - The impact of ethanol in vitro and in vivo: a comparative study KW - In Vivo KW - In Vitro KW - Ethanol Y1 - 2018 ER - TY - JOUR A1 - Ghalamkarpour, A. A1 - Holnthoner, Wolfgang A1 - Saharinen, Pipsa A1 - Boon, Laurence M A1 - Mulliken, J B A1 - Alitalo, Kari T1 - Recessive primary congenital lymphoedema caused by a VEGFR3 mutation JF - Journal of Medical Genetics KW - Lymphoedema Y1 - 2019 IS - 46(6) SP - 399 EP - 404 ER - TY - JOUR A1 - Bayer, Natascha A1 - Hirnschall, Nino A1 - Traxler, Lukas A1 - Drauschke, Andreas A1 - Find, Oliver T1 - Analysis of customized ocular data and wave front aberrations concerning their deviations from generic eye models JF - Proceedings der Dreiländertagung der biomedizinischen Technik (BMT) KW - Mechanical Eye Y1 - 2018 ER - TY - JOUR A1 - Sayer, Simon A1 - Zandrini, Tommaso A1 - Markovic, Marica A1 - Van Hoorick, Jasper A1 - Van Vlierberghe, Sandra A1 - Baudis, Stefan A1 - Holnthoner, Wolfgang A1 - Ovsianikov, Aleksandr T1 - Guiding cell migration in 3D with high-resolution photografting JF - Scientific Reports N2 - Multi-photon lithography (MPL) has proven to be a suitable tool to precisely control the microenvironment of cells in terms of the biochemical and biophysical properties of the hydrogel matrix. In this work, we present a novel method, based on multi-photon photografting of 4,4′-diazido-2,2′-stilbenedisulfonic acid (DSSA), and its capabilities to induce cell alignment, directional cell migration and endothelial sprouting in a gelatin-based hydrogel matrix. DSSA-photografting allows for the fabrication of complex patterns at a high-resolution and is a biocompatible, universally applicable and straightforward process that is comparably fast. We have demonstrated the preferential orientation of human adipose-derived stem cells (hASCs) in response to a photografted pattern. Co-culture spheroids of hASCs and human umbilical vein endothelial cells (HUVECs) have been utilized to study the directional migration of hASCs into the modified regions. Subsequently, we have highlighted the dependence of endothelial sprouting on the presence of hASCs and demonstrated the potential of photografting to control the direction of the sprouts. MPL-induced DSSA-photografting has been established as a promising method to selectively alter the microenvironment of cells. KW - Tissue Engineering KW - Cell migration KW - photografting Y1 - VL - 2022 IS - 12(1), 10196 SP - 8626 ER - TY - JOUR A1 - Hromada, Carina A1 - Hartmann, Jaana A1 - Oesterreicher, Johannes A1 - Stoiber, Anton A1 - Daerr, Anna A1 - Schädl, Barbara A1 - Priglinger, Eleni A1 - Teuschl-Woller, Andreas H. A1 - Holnthoner, Wolfgang A1 - Heinzel, Johannes Christoph A1 - Hercher, David T1 - Occurrence of Lymphangiogenesis in Peripheral Nerve Autografts Contrasts Schwann Cell-Induced Apoptosis of Lymphatic Endothelial Cells In Vitro JF - Biomolecules N2 - Peripheral nerve injuries pose a major clinical concern world-wide, and functional recovery after segmental peripheral nerve injury is often unsatisfactory, even in cases of autografting. Although it is well established that angiogenesis plays a pivotal role during nerve regeneration, the influence of lymphangiogenesis is strongly under-investigated. In this study, we analyzed the presence of lymphatic vasculature in healthy and regenerated murine peripheral nerves, revealing that nerve autografts contained increased numbers of lymphatic vessels after segmental damage. This led us to elucidate the interaction between lymphatic endothelial cells (LECs) and Schwann cells (SCs) in vitro. We show that SC and LEC secretomes did not influence the respective other cell types' migration and proliferation in 2D scratch assay experiments. Furthermore, we successfully created lymphatic microvascular structures in SC-embedded 3D fibrin hydrogels, in the presence of supporting cells; whereas SCs seemed to exert anti-lymphangiogenic effects when cultured with LECs alone. Here, we describe, for the first time, increased lymphangiogenesis after peripheral nerve injury and repair. Furthermore, our findings indicate a potential lymph-repellent property of SCs, thereby providing a possible explanation for the lack of lymphatic vessels in the healthy endoneurium. Our results highlight the importance of elucidating the molecular mechanisms of SC-LEC interaction. KW - Tissue Engineering KW - peripheral nerve regeneration KW - lymphangiogenesis KW - Schwann cells KW - lymphatic endothelial cells Y1 - VL - 2022 IS - 12, 6 SP - 820 ER - TY - JOUR A1 - Lauer, Henrik A1 - Prahm, Cosima A1 - Thiel, Johannes Tobias A1 - Kolbenschlag, Jonas A1 - Daigeler, Adrien A1 - Hercher, David A1 - Heinzel, Johannes Christoph T1 - The Grasping Test Revisited: A Systematic Review of Functional Recovery in Rat Models of Median Nerve Injury JF - Biomedicines N2 - The rat median nerve model is a well-established and frequently used model for peripheral nerve injury and repair. The grasping test is the gold-standard to evaluate functional recovery in this model. However, no comprehensive review exists to summarize the course of functional recovery in regard to the lesion type. According to PRISMA-guidelines, research was performed, including the databases PubMed and Web of Science. Groups were: (1) crush injury, (2) transection with end-to-end or with (3) end-to-side coaptation and (4) isogenic or acellular allogenic grafting. Total and respective number, as well as rat strain, type of nerve defect, length of isogenic or acellular allogenic allografts, time at first signs of motor recovery (FSR) and maximal recovery grasping strength (MRGS), were evaluated. In total, 47 articles met the inclusion criteria. Group I showed earliest signs of motor recovery. Slow recovery was observable in group III and in graft length above 25 mm. Isografts recovered faster compared to other grafts. The onset and course of recovery is heavily dependent from the type of nerve injury. The grasping test should be used complementary in addition to other volitional and non-volitional tests. Repetitive examinations should be planned carefully to optimize assessment of valid and reliable data. KW - Tissue Engineering KW - Median Nerve Injury KW - Nerve Regeneration Y1 - VL - 2022 IS - 10(8) SP - 1878 ER - TY - JOUR A1 - Rothbauer, Mario A1 - Byrne, Ruth A. A1 - Schobesberger, Silvia A1 - Olmos Calvo, Isabel A1 - Fischer, Anita A1 - Reihs, Eva I. A1 - Spitz, Sarah A1 - Bachmann, Barbara A1 - Sevelda, Florian A1 - Holinka, Johannes A1 - Holnthoner, Wolfgang A1 - Redl, Heinz A1 - Toegel, Stefan A1 - Windhager, Reinhard A1 - Kiener, Hans P. A1 - Ertl, Peter T1 - Establishment of a human three-dimensional chip-based chondro-synovial coculture joint model for reciprocal cross talk studies in arthritis research JF - Lab on a Chip N2 - Rheumatoid arthritis is characterised by a progressive, intermittent inflammation at the synovial membrane, which ultimately leads to the destruction of the synovial joint. The synovial membrane as the joint capsule's inner layer is lined with fibroblast-like synoviocytes that are the key player supporting persistent arthritis leading to bone erosion and cartilage destruction. While microfluidic models that model molecular aspects of bone erosion between bone-derived cells and synoviocytes have been established, RA's synovial-chondral axis has not yet been realised using a microfluidic 3D model based on human patient in vitro cultures. Consequently, we established a chip-based three-dimensional tissue coculture model that simulates the reciprocal cross talk between individual synovial and chondral organoids. When co-cultivated with synovial organoids, we could demonstrate that chondral organoids induce a higher degree of cartilage physiology and architecture and show differential cytokine response compared to their respective monocultures highlighting the importance of reciprocal tissue-level cross talk in the modelling of arthritic diseases. KW - Tissue Engineering KW - coculture joint model KW - arthritis KW - human three-dimensional chip Y1 - VL - 2021 IS - 21 SP - 4128 EP - 4143 ER - TY - GEN A1 - Liousia, Varvara A1 - Rünzler, Dominik T1 - Stage- and dose-dependent effects of methanol and ethanol on the locomotor activity of zebrafish larvae KW - Locomotor Activity KW - Zebrafish Y1 - 2018 ER - TY - JOUR A1 - Henderson, Ben A1 - Slingers, Gitte A1 - Pedrotti, Michele A1 - Pugliese, Giovanni A1 - Malaskova, Michaela A1 - Bryant, Luke A1 - Lomonaco, Tommaso A1 - Ghimenti, Silvia A1 - Moreno, Sergi A1 - Cordell, Rebecca A1 - Harren, Frans J M A1 - Schubert, Jochen A1 - Mayhew, Chris A A1 - Wilde, Michael A1 - Di Francesco, Fabio A1 - Koppen, Gudrun A1 - Beauchamp, Jonathan D A1 - Cristescu, Simona M T1 - The peppermint breath test benchmark for PTR-MS and SIFT-MS JF - Journal of Breath Research N2 - A major challenge for breath research is the lack of standardization in sampling and analysis. To address this, a test that utilizes a standardized intervention and a defined study protocol has been proposed to explore disparities in breath research across different analytical platforms and to provide benchmark values for comparison. Specifically, the Peppermint Experiment involves the targeted analysis in exhaled breath of volatile constituents of peppermint oil after ingestion of the encapsulated oil. Data from the Peppermint Experiment performed by proton transfer reaction mass spectrometry (PTR-MS) and selected ion flow tube mass spectrometry (SIFT-MS) are presented and discussed herein, including the product ions associated with the key peppermint volatiles, namely limonene, α- and β-pinene, 1,8-cineole, menthol, menthone and menthofuran. The breath washout profiles of these compounds from 65 individuals were collected, comprising datasets from five PTR-MS and two SIFT-MS instruments. The washout profiles of these volatiles were evaluated by comparing the log-fold change over time of the product ion intensities associated with each volatile. Benchmark values were calculated from the lower 95% confidence interval of the linear time-to-washout regression analysis for all datasets combined. Benchmark washout values from PTR-MS analysis were 353 min for the sum of monoterpenes and 1,8-cineole (identical product ions), 173 min for menthol, 330 min for menthofuran, and 218 min for menthone; from SIFT-MS analysis values were 228 min for the sum of monoterpenes, 281 min for the sum of monoterpenes and 1,8-cineole, and 370 min for menthone plus 1,8-cineole. Large inter- and intra-dataset variations were observed, whereby the latter suggests that biological variability plays a key role in how the compounds are absorbed, metabolized and excreted from the body via breath. This variability seems large compared to the influence of sampling and analytical procedures, but further investigations are recommended to clarify the effects of these factors. KW - standardization KW - breathomics KW - peppermint KW - benchmark KW - TR-MS Y1 - U6 - http://dx.doi.org/https://doi.org/10.1088/1752-7163/ac1fcf IS - 15 SP - Artikelnr. 046005 ER - TY - GEN A1 - Friedrich, Robin A1 - Lakic, Nevana A1 - Prähauser, Linda A1 - Schweitzer, Karoline A1 - Olscher, Christoph A1 - Monforte Vila, Xavier A1 - Leitner, Rita A1 - Gepp, Barbara T1 - Effects of Plastic on the Freshwater Snail Biomphalaria Glabrata T2 - SETAC Europe 32nd Annual Meeting in Copenhagen, Denmark from 15 - 19. May 2022 KW - Ecotoxicology KW - Biomphalaria Glabrata Y1 - ER - TY - GEN A1 - Nemec, Iris A1 - Frohner, Matthias T1 - How can life science students, especially biomedical engineering students, benefit from the extra-curricular offerings and systems already established in other scientific fields? T2 - Abstracts of the 2022 Joint Annual Conference of the Austrian (ÖGBMT), German (VDE DGBMT) and Swiss (SSBE) Societies for Biomedical Engineering, including the 14th Vienna International Workshop on Functional Electrical Stimulation KW - extra-curricular offerings KW - education Y1 - U6 - http://dx.doi.org/https://doi.org/10.1515/bmt-2022-2001 SP - 348 ER - TY - GEN A1 - Nemec, Iris A1 - Malaskova, Michaela A1 - Pereira, Luis A1 - Pavao, Joao A1 - Frohner, Matthias T1 - Experiences of intercultural teaching activities in the field of eHealth T2 - Abstracts of the 2022 Joint Annual Conference of the Austrian (ÖGBMT), German (VDE DGBMT) and Swiss (SSBE) Societies for Biomedical Engineering, including the 14th Vienna International Workshop on Functional Electrical Stimulation KW - intercultural teaching KW - eHealth Y1 - U6 - http://dx.doi.org/https://doi.org/10.1515/bmt-2022-2001 SP - 351 ER - TY - GEN A1 - Traxler, Lukas A1 - Balz, Andrea T1 - Current Advances in the Optical Characterization of Intraocular Lenses T2 - Abstracts of the 2022 Joint Annual Conference of the Austrian (ÖGBMT), German (VDE DGBMT) and Swiss (SSBE) Societies for Biomedical Engineering, including the 14th Vienna International Workshop on Functional Electrical Stimulation KW - Intraocular Lenses Y1 - U6 - http://dx.doi.org/https://doi.org/10.1515/bmt-2022-2001 SP - 102 ER - TY - JOUR A1 - Ashmwe, Mohamed A1 - Posa, Katja A1 - Rührnößl, Alexander A1 - Heinzel, Johannes Christoph A1 - Heimel, Patrick A1 - Mock, Michael A1 - Schädl, Barbara A1 - Keibl, Claudia A1 - Couillard-Despres, Sebastien A1 - Redl, Heinz A1 - Mittermayr, Rainer A1 - Hercher, David T1 - Effects of Extracorporeal Shockwave Therapy on Functional Recovery and Circulating miR-375 and miR-382-5p after Subacute and Chronic Spinal Cord Contusion Injury in Rats JF - Biomedicines N2 - Extracorporeal shockwave therapy (ESWT) can stimulate processes to promote regeneration, including cell proliferation and modulation of inflammation. Specific miRNA expression panels have been established to define correlations with regulatory targets within these pathways. This study aims to investigate the influence of low-energy ESWT-applied within the subacute and chronic phase of SCI (spinal cord injury) on recovery in a rat spinal cord contusion model. Outcomes were evaluated by gait analysis, µCT and histological analysis of spinal cords. A panel of serum-derived miRNAs after SCI and after ESWT was investigated to identify injury-, regeneration- and treatment-associated expression patterns. Rats receiving ESWT showed significant improvement in motor function in both a subacute and a chronic experimental setting. This effect was not reflected in changes in morphology, µCT-parameters or histological markers after ESWT. Expression analysis of various miRNAs, however, revealed changes after SCI and ESWT, with increased miR-375, indicating a neuroprotective effect, and decreased miR-382-5p potentially improving neuroplasticity via its regulatory involvement with BDNF. We were able to demonstrate a functional improvement of ESWT-treated animals after SCI in a subacute and chronic setting. Furthermore, the identification of miR-375 and miR-382-5p could potentially provide new targets for therapeutic intervention in future studies. KW - Tissue Engineering KW - ESWT KW - Spinal Cord Injury Y1 - U6 - http://dx.doi.org/https://doi.org/10.3390/biomedicines10071630 VL - 2022 IS - 10(7) SP - 1630 ER - TY - JOUR A1 - Hanetseder, Dominik A1 - Levstek, Tina A1 - Teuschl-Woller, Andreas A1 - Frank, Julia Katharina A1 - Schaedl, Barbara A1 - Redl, Heinz A1 - Marolt Presen, Darja T1 - Engineering of extracellular matrix from human iPSC-mesenchymal progenitors to enhance osteogenic capacity of human bone marrow stromal cells independent of their age JF - Front Bioeng Biotechnol N2 - Regeneration of bone defects is often limited due to compromised bone tissue physiology. Previous studies suggest that engineered extracellular matrices enhance the regenerative capacity of mesenchymal stromal cells. In this study, we used human-induced pluripotent stem cells, a scalable source of young mesenchymal progenitors (hiPSC-MPs), to generate extracellular matrix (iECM) and test its effects on the osteogenic capacity of human bone-marrow mesenchymal stromal cells (BMSCs). iECM was deposited as a layer on cell culture dishes and into three-dimensional (3D) silk-based spongy scaffolds. After decellularization, iECM maintained inherent structural proteins including collagens, fibronectin and laminin, and contained minimal residual DNA. Young adult and aged BMSCs cultured on the iECM layer in osteogenic medium exhibited a significant increase in proliferation, osteogenic marker expression, and mineralization as compared to tissue culture plastic. With BMSCs from aged donors, matrix mineralization was only detected when cultured on iECM, but not on tissue culture plastic. When cultured in 3D iECM/silk scaffolds, BMSCs exhibited significantly increased osteogenic gene expression levels and bone matrix deposition. iECM layer showed a similar enhancement of aged BMSC proliferation, osteogenic gene expression, and mineralization compared with extracellular matrix layers derived from young adult or aged BMSCs. However, iECM increased osteogenic differentiation and decreased adipocyte formation compared with single protein substrates including collagen and fibronectin. Together, our data suggest that the microenvironment comprised of iECM can enhance the osteogenic activity of BMSCs, providing a bioactive and scalable biomaterial strategy for enhancing bone regeneration in patients with delayed or failed bone healing. KW - aging KW - iPSCs KW - osteogenic differentiation KW - bone marrow stromal cells KW - extracellular matrix Y1 - U6 - http://dx.doi.org/https://doi.org/10.3389/fbioe.2023.1214019 VL - 11 ER - TY - JOUR A1 - Bernhard, Jonathan C A1 - Marolt Presen, Darja A1 - Li, Ming A1 - Monforte, Xavier A1 - Ferguson, James A1 - Leinfellner, Gabriele A1 - Heimel, Patrick A1 - Betti, Susanne L A1 - Shu, Sharon A1 - Teuschl-Woller, Andreas H A1 - Tangl, Stefan A1 - Redl, Heinz A1 - Vunjak-Novakovic, Gordana T1 - Effects of Endochondral and Intramembranous Ossification Pathways on Bone Tissue Formation and Vascularization in Human Tissue-Engineered Grafts JF - Cells N2 - Bone grafts can be engineered by differentiating human mesenchymal stromal cells (MSCs) via the endochondral and intramembranous ossification pathways. We evaluated the effects of each pathway on the properties of engineered bone grafts and their capacity to drive bone regeneration. Bone-marrow-derived MSCs were differentiated on silk scaffolds into either hypertrophic chondrocytes (hyper) or osteoblasts (osteo) over 5 weeks of in vitro cultivation, and were implanted subcutaneously for 12 weeks. The pathways' constructs were evaluated over time with respect to gene expression, composition, histomorphology, microstructure, vascularization and biomechanics. Hypertrophic chondrocytes expressed higher levels of osteogenic genes and deposited significantly more bone mineral and proteins than the osteoblasts. Before implantation, the mineral in the hyper group was less mature than that in the osteo group. Following 12 weeks of implantation, the hyper group had increased mineral density but a similar overall mineral composition compared with the osteo group. The hyper group also displayed significantly more blood vessel infiltration than the osteo group. Both groups contained M2 macrophages, indicating bone regeneration. These data suggest that, similar to the body's repair processes, endochondral pathway might be more advantageous when regenerating large defects, whereas intramembranous ossification could be utilized to guide the tissue formation pattern with a scaffold architecture. KW - bone tissue engineering KW - endochondral KW - mesenchymal stromal cells KW - ossification KW - intramembranous Y1 - U6 - http://dx.doi.org/10.3390/cells11193070 VL - 11 IS - 19:3070 ER - TY - JOUR A1 - Gollmann-Tepeköylü, Can A1 - Graber, Michael A1 - Hirsch, Jakob A1 - Mair, Sophia A1 - Naschberger, Andreas A1 - Pölzl, Leo A1 - Nägele, Felix A1 - Kirchmair, Elke A1 - Degenhart, Gerald A1 - Demetz, Egon A1 - Hilbe, Richard A1 - Chen, Hao-Yu A1 - Engert, James C A1 - Böhm, Anna A1 - Franz, Nadja A1 - Lobenwein, Daniela A1 - Lener, Daniela A1 - Fuchs, Christiane A1 - Weihs, Anna A1 - Töchterle, Sonja A1 - Vogel, Georg F A1 - Schweiger, Victor A1 - Eder, Jonas A1 - Pietschmann, Peter A1 - Seifert, Markus A1 - Kronenberg, Florian A1 - Coassin, Stefan A1 - Blumer, Michael A1 - Hackl, Hubert A1 - Meyer, Dirk A1 - Feuchtner, Gudrun A1 - Kirchmair, Rudolf A1 - Troppmair, Jakob A1 - Krane, Markus A1 - Weiss, Günther A1 - Tsimikas, Sotirios A1 - Thanassoulis, George A1 - Grimm, Michael A1 - Rupp, Bernhard A1 - Huber, Lukas A A1 - Zhang, Shen-Ying A1 - Casanova, Jean-Laurent A1 - Tancevski, Ivan A1 - Holfeld, Johannes T1 - Toll-Like Receptor 3 Mediates Aortic Stenosis Through a Conserved Mechanism of Calcification JF - Circulation KW - Toll-like receptor 3 KW - aortic valve disease KW - biglycan KW - extracellular matrix KW - osteogenesis Y1 - U6 - http://dx.doi.org/10.1161/CIRCULATIONAHA.122.063481 VL - 147 IS - 20 SP - 1518 EP - 1533 ER - TY - GEN A1 - Balz, Andrea A1 - Langer, Sarah A1 - Frohner, Matthias A1 - Forjan, Mathias T1 - Development of Internationalized Teaching and Training Modules for Healthcare Professionals T2 - Abstracts of the 2022 Joint Annual Conference of the Austrian (ÖGBMT), German (VDE DGBMT) and Swiss (SSBE) Societies for Biomedical Engineering, including the 14th Vienna International Workshop on Functional Electrical Stimulation KW - Internationalized Teaching KW - Healthcare Y1 - U6 - http://dx.doi.org/https://doi.org/10.1515/bmt-2022-2001 SP - 353 ER - TY - JOUR A1 - Xu, Yingyang A1 - Gepp, Barbara A1 - Lengger, Nina A1 - Yin, Jia A1 - Breiteneder, Heimo T1 - Identification of probable pectinesterase as a major allergen of pollen of the Asian white birch (Betula platyphylla) in northern China JF - Asian Pac J Allergy Immunol KW - birch pollen allergy KW - betula platyphylla KW - chinese population KW - major allergen KW - pectinesterase Y1 - U6 - http://dx.doi.org/10.12932/AP-100722-1409 ER - TY - GEN A1 - Eidi, Nada A1 - Balz, Andrea A1 - Forjan, Mathias T1 - MedTech-mR - Creating a Virtual Enviroment for Medical Training and Room Planning T2 - Abstracts of the 2022 Joint Annual Conference of the Austrian (ÖGBMT), German (VDE DGBMT) and Swiss (SSBE) Societies for Biomedical Engineering, including the 14th Vienna International Workshop on Functional Electrical Stimulation KW - Medical Training KW - Virtual Environment Y1 - U6 - http://dx.doi.org/https://doi.org/10.1515/bmt-2022-2001 SP - 349 ER - TY - GEN A1 - Malaskova, Michaela A1 - Forjan, Mathias T1 - Current topics in Life Science Engineering lectures T2 - Abstracts of the 2022 Joint Annual Conference of the Austrian (ÖGBMT), German (VDE DGBMT) and Swiss (SSBE) Societies for Biomedical Engineering, including the 14th Vienna International Workshop on Functional Electrical Stimulation KW - Life Science Engineering KW - Teaching Y1 - U6 - http://dx.doi.org/https://doi.org/10.1515/bmt-2022-2001 SP - 350 ER - TY - JOUR A1 - Romanelli, Pasquale A1 - Bieler, Lara A1 - Heimel, Patrick A1 - Škokić, Siniša A1 - Jakubecova, Dominika A1 - Kreutzer, Christina A1 - Zaunmair, Pia A1 - Smolčić, Tomislav A1 - Benedetti, Bruno A1 - Rohde, Eva A1 - Gimona, Mario A1 - Hercher, David A1 - Dobrivojević Radmilović, Marina A1 - Couillard-Despres, Sebastien T1 - Enhancing Functional Recovery Through Intralesional Application of Extracellular Vesicles in a Rat Model of Traumatic Spinal Cord Injury JF - Front Cell Neurosci N2 - Local inflammation plays a pivotal role in the process of secondary damage after spinal cord injury. We recently reported that acute intravenous application of extracellular vesicles (EVs) secreted by human umbilical cord mesenchymal stromal cells dampens the induction of inflammatory processes following traumatic spinal cord injury. However, systemic application of EVs is associated with delayed delivery to the site of injury and the necessity for high doses to reach therapeutic levels locally. To resolve these two constraints, we injected EVs directly at the lesion site acutely after spinal cord injury. We report here that intralesional application of EVs resulted in a more robust improvement of motor recovery, assessed with the BBB score and sub-score, as compared to the intravenous delivery. Moreover, the intralesional application was more potent in reducing inflammation and scarring after spinal cord injury than intravenous administration. Hence, the development of EV-based therapy for spinal cord injury should aim at an early application of vesicles close to the lesion. KW - exosomes KW - inflammation KW - locomotion KW - neuroimaging KW - motor function Y1 - U6 - http://dx.doi.org/10.3389/fncel.2021.795008 VL - 15 ER - TY - JOUR A1 - Feichtinger, Xaver A1 - Heimel, Patrick A1 - Tangl, Stefan A1 - Keibl, Claudia A1 - Nürnberger, Sylvia A1 - Schanda, Jakob Emanuel A1 - Hercher, David A1 - Kocijan, Roland A1 - Redl, Heinz A1 - Grillari, Johannes A1 - Fialka, Christian A1 - Mittermayr, Rainer T1 - Improved biomechanics in experimental chronic rotator cuff repair after shockwaves is not reflected by bone microarchitecture JF - PLoS One KW - chronic rotator cuff repair KW - bone microarchitecture Y1 - U6 - http://dx.doi.org/10.1371/journal.pone.0262294 VL - 17 IS - 1 ER - TY - THES A1 - Tomasch, Janine T1 - Strategies to improve the myogenic outcome of skeletal muscle tissue engineering approaches through optimization of biomaterial properties and mechanical stimuli KW - muscle KW - bioreactor KW - tissue engineering KW - fibrin KW - biomaterial Y1 - ER - TY - GEN A1 - Langer, Sarah A1 - Nemec, Iris A1 - Kollmitzer, Josef A1 - Scherer, Matthias A1 - Martinek, Johannes T1 - Vergleich des decompositionierten und des überlagerten Elektromyogramms zur Analyse der Muskelermüdung N2 - Ziel dieser Studie war das überlagerte Elektromyogramm (ueEMG) und das decompositionierte (dEMG) auf deren Reliabilität und Aussagekraft zu überprüfen. Hierfür vollzogen zehn gesunde männliche Probanden zu drei Testzeitpunkten isometrische Ermüdungsmessungen. Hintergrund Bei der Messung des ueEMG werden die Motor Units Aktionspotentiale (MUAPS) elektrisch überlagert. Physiologische Muskelermüdung zeigt sich hier unter anderem in der Veränderung des Frequenzspektrums und der Signalamplitude. Letzteres steigt bei Ermüdung an, wohingegen die mediane Frequenz (MF) sinkt. Eine weitere Möglichkeit die Muskelermüdung mittels Oberflächen-EMG zu ermitteln ist die Anwendung des dEMGs. Dieses ermöglicht mithilfe einer Mustererkennungssoftware einzelne MUAPS zu erkennen. Studien zeigten, dass aktive Motor Units (MU) bei wiederholten oder anhaltenden submaximalen Kontraktionen als Muskelermüdung ihre Feuerungsrate erhöhen und neue MU rekrutiert werden. Bisher wurden diese Methoden jeweils einzeln betrachtet. Um sicherzustellen welcher Ansatz für klinische Daten geeigneter ist, wurden Datensätze zu beiden Methoden gleichzeitig generiert und hinsichtlich ihrer Aussagekraft und Wiederholbarkeit mit statistischen Mitteln verglichen. KW - Elektromyogramm KW - Rehabilitationstechnik Y1 - 2022 ER -