TY - JOUR A1 - Wala, Thomas A1 - Felleitner-Goll, Katharina T1 - Digitalisierung in der Weiterbildung. Wie man mit eigenen Online-Kursen ein passives Zusatzeinkommen generieren kann. JF - ASoK KW - Weiterbildung KW - Digitalisierung Y1 - IS - 5 SP - 189 EP - 191 ER - TY - JOUR A1 - Wala, Thomas A1 - Felleitner-Goll, Katharina T1 - Technologiemanagement JF - WISU KW - Technologiemanagement Y1 - IS - 4 SP - 349 EP - 350 ER - TY - JOUR A1 - Kierspel, Thomas A1 - Kadek, Alan A1 - Barran, Perdita A1 - Bellina, Bruno A1 - Bijedic, Adi A1 - Brodmerkel, Maxim N. A1 - Commandeur, Jan A1 - Caleman, Carl A1 - Damjanovic, Tomislav A1 - Dawod, Ibrahim A1 - De Santis, Emiliano A1 - Lekkas, Alexandros A1 - Lorenzen, Kristina A1 - López Morillo, Luis A1 - Mandl, Thomas A1 - Marklund, Erik G. A1 - Papanastasiou, Dimitris A1 - Ramakers, Lennart A. I. A1 - Schweikhard, Lutz A1 - Simke, Florian A1 - Sinelnikova, Anna A1 - Smyrnakis, Athanasios A1 - Timneanu, Nicusor A1 - Uetrecht, Charlotte T1 - Coherent diffractive imaging of proteins and viral capsids: simulating MS SPIDOC JF - Analytical and Bioanalytical Chemistry N2 - MS SPIDOC is a novel sample delivery system designed for single (isolated) particle imaging at X-ray Free-Electron Lasers that is adaptable towards most large-scale facility beamlines. Biological samples can range from small proteins to MDa particles. Following nano-electrospray ionization, ionic samples can be m/z-filtered and structurally separated before being oriented at the interaction zone. Here, we present the simulation package developed alongside this prototype. The first part describes how the front-to-end ion trajectory simulations have been conducted. Highlighted is a quadrant lens; a simple but efficient device that steers the ion beam within the vicinity of the strong DC orientation field in the interaction zone to ensure spatial overlap with the X-rays. The second part focuses on protein orientation and discusses its potential with respect to diffractive imaging methods. Last, coherent diffractive imaging of prototypical T = 1 and T = 3 norovirus capsids is shown. We use realistic experimental parameters from the SPB/SFX instrument at the European XFEL to demonstrate that low- resolution diffractive imaging data (q < 0.3 nm −1 ) can be collected with only a few X-ray pulses. Such low-resolution data are sufficient to distinguish between both symmetries of the capsids, allowing to probe low abundant species in a beam if MS SPIDOC is used as sample delivery. KW - SPI KW - X-ray KW - Native MS KW - Protein complex structure KW - Simulation Y1 - U6 - http://dx.doi.org/https://doi.org/10.1007/s00216-023-04658-y VL - 2023 IS - 415 SP - 4209 EP - 4220 ER - TY - JOUR A1 - Hanetseder, Dominik A1 - Levstek, Tina A1 - Teuschl-Woller, Andreas A1 - Frank, Julia Katharina A1 - Schaedl, Barbara A1 - Redl, Heinz A1 - Marolt Presen, Darja T1 - Engineering of extracellular matrix from human iPSC-mesenchymal progenitors to enhance osteogenic capacity of human bone marrow stromal cells independent of their age JF - Front Bioeng Biotechnol N2 - Regeneration of bone defects is often limited due to compromised bone tissue physiology. Previous studies suggest that engineered extracellular matrices enhance the regenerative capacity of mesenchymal stromal cells. In this study, we used human-induced pluripotent stem cells, a scalable source of young mesenchymal progenitors (hiPSC-MPs), to generate extracellular matrix (iECM) and test its effects on the osteogenic capacity of human bone-marrow mesenchymal stromal cells (BMSCs). iECM was deposited as a layer on cell culture dishes and into three-dimensional (3D) silk-based spongy scaffolds. After decellularization, iECM maintained inherent structural proteins including collagens, fibronectin and laminin, and contained minimal residual DNA. Young adult and aged BMSCs cultured on the iECM layer in osteogenic medium exhibited a significant increase in proliferation, osteogenic marker expression, and mineralization as compared to tissue culture plastic. With BMSCs from aged donors, matrix mineralization was only detected when cultured on iECM, but not on tissue culture plastic. When cultured in 3D iECM/silk scaffolds, BMSCs exhibited significantly increased osteogenic gene expression levels and bone matrix deposition. iECM layer showed a similar enhancement of aged BMSC proliferation, osteogenic gene expression, and mineralization compared with extracellular matrix layers derived from young adult or aged BMSCs. However, iECM increased osteogenic differentiation and decreased adipocyte formation compared with single protein substrates including collagen and fibronectin. Together, our data suggest that the microenvironment comprised of iECM can enhance the osteogenic activity of BMSCs, providing a bioactive and scalable biomaterial strategy for enhancing bone regeneration in patients with delayed or failed bone healing. KW - aging KW - iPSCs KW - osteogenic differentiation KW - bone marrow stromal cells KW - extracellular matrix Y1 - U6 - http://dx.doi.org/https://doi.org/10.3389/fbioe.2023.1214019 VL - 11 ER - TY - JOUR A1 - Bernhard, Jonathan C A1 - Marolt Presen, Darja A1 - Li, Ming A1 - Monforte, Xavier A1 - Ferguson, James A1 - Leinfellner, Gabriele A1 - Heimel, Patrick A1 - Betti, Susanne L A1 - Shu, Sharon A1 - Teuschl-Woller, Andreas H A1 - Tangl, Stefan A1 - Redl, Heinz A1 - Vunjak-Novakovic, Gordana T1 - Effects of Endochondral and Intramembranous Ossification Pathways on Bone Tissue Formation and Vascularization in Human Tissue-Engineered Grafts JF - Cells N2 - Bone grafts can be engineered by differentiating human mesenchymal stromal cells (MSCs) via the endochondral and intramembranous ossification pathways. We evaluated the effects of each pathway on the properties of engineered bone grafts and their capacity to drive bone regeneration. Bone-marrow-derived MSCs were differentiated on silk scaffolds into either hypertrophic chondrocytes (hyper) or osteoblasts (osteo) over 5 weeks of in vitro cultivation, and were implanted subcutaneously for 12 weeks. The pathways' constructs were evaluated over time with respect to gene expression, composition, histomorphology, microstructure, vascularization and biomechanics. Hypertrophic chondrocytes expressed higher levels of osteogenic genes and deposited significantly more bone mineral and proteins than the osteoblasts. Before implantation, the mineral in the hyper group was less mature than that in the osteo group. Following 12 weeks of implantation, the hyper group had increased mineral density but a similar overall mineral composition compared with the osteo group. The hyper group also displayed significantly more blood vessel infiltration than the osteo group. Both groups contained M2 macrophages, indicating bone regeneration. These data suggest that, similar to the body's repair processes, endochondral pathway might be more advantageous when regenerating large defects, whereas intramembranous ossification could be utilized to guide the tissue formation pattern with a scaffold architecture. KW - bone tissue engineering KW - endochondral KW - mesenchymal stromal cells KW - ossification KW - intramembranous Y1 - U6 - http://dx.doi.org/10.3390/cells11193070 VL - 11 IS - 19:3070 ER - TY - JOUR A1 - Gollmann-Tepeköylü, Can A1 - Graber, Michael A1 - Hirsch, Jakob A1 - Mair, Sophia A1 - Naschberger, Andreas A1 - Pölzl, Leo A1 - Nägele, Felix A1 - Kirchmair, Elke A1 - Degenhart, Gerald A1 - Demetz, Egon A1 - Hilbe, Richard A1 - Chen, Hao-Yu A1 - Engert, James C A1 - Böhm, Anna A1 - Franz, Nadja A1 - Lobenwein, Daniela A1 - Lener, Daniela A1 - Fuchs, Christiane A1 - Weihs, Anna A1 - Töchterle, Sonja A1 - Vogel, Georg F A1 - Schweiger, Victor A1 - Eder, Jonas A1 - Pietschmann, Peter A1 - Seifert, Markus A1 - Kronenberg, Florian A1 - Coassin, Stefan A1 - Blumer, Michael A1 - Hackl, Hubert A1 - Meyer, Dirk A1 - Feuchtner, Gudrun A1 - Kirchmair, Rudolf A1 - Troppmair, Jakob A1 - Krane, Markus A1 - Weiss, Günther A1 - Tsimikas, Sotirios A1 - Thanassoulis, George A1 - Grimm, Michael A1 - Rupp, Bernhard A1 - Huber, Lukas A A1 - Zhang, Shen-Ying A1 - Casanova, Jean-Laurent A1 - Tancevski, Ivan A1 - Holfeld, Johannes T1 - Toll-Like Receptor 3 Mediates Aortic Stenosis Through a Conserved Mechanism of Calcification JF - Circulation KW - Toll-like receptor 3 KW - aortic valve disease KW - biglycan KW - extracellular matrix KW - osteogenesis Y1 - U6 - http://dx.doi.org/10.1161/CIRCULATIONAHA.122.063481 VL - 147 IS - 20 SP - 1518 EP - 1533 ER - TY - JOUR A1 - Xu, Yingyang A1 - Gepp, Barbara A1 - Lengger, Nina A1 - Yin, Jia A1 - Breiteneder, Heimo T1 - Identification of probable pectinesterase as a major allergen of pollen of the Asian white birch (Betula platyphylla) in northern China JF - Asian Pac J Allergy Immunol KW - birch pollen allergy KW - betula platyphylla KW - chinese population KW - major allergen KW - pectinesterase Y1 - U6 - http://dx.doi.org/10.12932/AP-100722-1409 ER - TY - JOUR A1 - Ahlborn, Felix A1 - Kupka, Friedrich A1 - Weiss, Achim A1 - Flaskamp, Martin T1 - Stellar evolution models with overshooting based on 3-equation non-local theories: II. Main sequence models of A- and B-type stars JF - Astronomy & Astrophysics KW - convection KW - turbulence KW - stars: evolution KW - stars: interiors Y1 - U6 - http://dx.doi.org/https://doi.org/10.1051/0004-6361/202243126 IS - Volume 667 SP - Article Number A97 ER - TY - JOUR A1 - Kupka, Friedrich A1 - Ahlborn, Felix A1 - Weiss, Achim T1 - Stellar evolution models with overshooting based on 3-equation non-local theories: I. Physical basics and the computation of the dissipation rate JF - Astronomy & Astrophysics KW - convection KW - turbulence KW - stars: evolution KW - stars: interiors Y1 - U6 - http://dx.doi.org/https://doi.org/10.1051/0004-6361/202243125 IS - Volume 667 SP - Article Number A96 ER - TY - JOUR A1 - Huber, Albert T1 - Quasilocal corrections to Bondi’s mass-loss formula and dynamical horizons JF - Physical Review D KW - Bondi´s mass-loss formula KW - quasilocal KW - dynamical horizons Y1 - U6 - http://dx.doi.org/https://doi.org/10.1103/PhysRevD.108.084056 VL - Vol. 108 IS - issue 8 ER -