TY  - JOUR
A1  - Tomasch, Janine
A1  - Maleiner, Babette
A1  - Hromada, Carina
A1  - Szwarc-Hofbauer, Dorota
A1  - Teuschl-Woller, Andreas
T1  - Cyclic Tensile Stress Induces Skeletal Muscle Hypertrophy and Myonuclear Accretion in a 3D Model
JF  - Tissue Eng. Part A.
N2  - Skeletal muscle is highly adaptive to mechanical stress due to its resident stem cells and the pronounced level of myotube plasticity. Herein, we study the adaptation to mechanical stress and its underlying molecular mechanisms in a tissue-engineered skeletal muscle model. We subjected differentiated 3D skeletal muscle-like constructs to cyclic tensile stress using a custom-made bioreactor system, which resulted in immediate activation of stress-related signal transducers (Erk1/2, p38). Cell cycle re-entry, increased proliferation, and onset of myogenesis indicated subsequent myoblast activation. Furthermore, elevated focal adhesion kinase and β-catenin activity in mechanically stressed constructs suggested increased cell adhesion and migration. After 3 days of mechanical stress, gene expression of the fusogenic markers MyoMaker and MyoMixer, myotube diameter, myonuclear accretion, as well as S6 activation, were significantly increased. Our results highlight that we established a promising tool to study sustained adaptation to mechanical stress in healthy, hypertrophic, or regenerating skeletal muscle.
KW  - fibrin
KW  - tissue engineering
KW  - tensile stress
KW  - regeneration
KW  - hypertrophy
Y1  - 
VL  - 2023
IS  - Mar
SP  - 257
EP  - 268
ER  - 
TY  - THES
A1  - Tomasch, Janine
T1  - Strategies to improve the myogenic outcome of skeletal muscle tissue engineering approaches through optimization of biomaterial properties and mechanical stimuli
KW  - muscle
KW  - bioreactor
KW  - tissue engineering
KW  - fibrin
KW  - biomaterial
Y1  - 
ER  -