TY - JOUR A1 - Tomasch, Janine A1 - Maleiner, Babette A1 - Hromada, Carina A1 - Szwarc-Hofbauer, Dorota A1 - Teuschl-Woller, Andreas T1 - Cyclic Tensile Stress Induces Skeletal Muscle Hypertrophy and Myonuclear Accretion in a 3D Model JF - Tissue Eng. Part A. N2 - Skeletal muscle is highly adaptive to mechanical stress due to its resident stem cells and the pronounced level of myotube plasticity. Herein, we study the adaptation to mechanical stress and its underlying molecular mechanisms in a tissue-engineered skeletal muscle model. We subjected differentiated 3D skeletal muscle-like constructs to cyclic tensile stress using a custom-made bioreactor system, which resulted in immediate activation of stress-related signal transducers (Erk1/2, p38). Cell cycle re-entry, increased proliferation, and onset of myogenesis indicated subsequent myoblast activation. Furthermore, elevated focal adhesion kinase and β-catenin activity in mechanically stressed constructs suggested increased cell adhesion and migration. After 3 days of mechanical stress, gene expression of the fusogenic markers MyoMaker and MyoMixer, myotube diameter, myonuclear accretion, as well as S6 activation, were significantly increased. Our results highlight that we established a promising tool to study sustained adaptation to mechanical stress in healthy, hypertrophic, or regenerating skeletal muscle. KW - fibrin KW - tissue engineering KW - tensile stress KW - regeneration KW - hypertrophy Y1 - VL - 2023 IS - Mar SP - 257 EP - 268 ER - TY - GEN A1 - Nemec, Iris A1 - Frohner, Matthias T1 - How can life science students, especially biomedical engineering students, benefit from the extra-curricular offerings and systems already established in other scientific fields? T2 - Abstracts of the 2022 Joint Annual Conference of the Austrian (ÖGBMT), German (VDE DGBMT) and Swiss (SSBE) Societies for Biomedical Engineering, including the 14th Vienna International Workshop on Functional Electrical Stimulation KW - extra-curricular offerings KW - education Y1 - U6 - http://dx.doi.org/https://doi.org/10.1515/bmt-2022-2001 SP - 348 ER - TY - GEN A1 - Nemec, Iris A1 - Malaskova, Michaela A1 - Pereira, Luis A1 - Pavao, Joao A1 - Frohner, Matthias T1 - Experiences of intercultural teaching activities in the field of eHealth T2 - Abstracts of the 2022 Joint Annual Conference of the Austrian (ÖGBMT), German (VDE DGBMT) and Swiss (SSBE) Societies for Biomedical Engineering, including the 14th Vienna International Workshop on Functional Electrical Stimulation KW - intercultural teaching KW - eHealth Y1 - U6 - http://dx.doi.org/https://doi.org/10.1515/bmt-2022-2001 SP - 351 ER - TY - GEN A1 - Traxler, Lukas A1 - Balz, Andrea T1 - Current Advances in the Optical Characterization of Intraocular Lenses T2 - Abstracts of the 2022 Joint Annual Conference of the Austrian (ÖGBMT), German (VDE DGBMT) and Swiss (SSBE) Societies for Biomedical Engineering, including the 14th Vienna International Workshop on Functional Electrical Stimulation KW - Intraocular Lenses Y1 - U6 - http://dx.doi.org/https://doi.org/10.1515/bmt-2022-2001 SP - 102 ER - TY - JOUR A1 - Hanetseder, Dominik A1 - Levstek, Tina A1 - Teuschl-Woller, Andreas A1 - Frank, Julia Katharina A1 - Schaedl, Barbara A1 - Redl, Heinz A1 - Marolt Presen, Darja T1 - Engineering of extracellular matrix from human iPSC-mesenchymal progenitors to enhance osteogenic capacity of human bone marrow stromal cells independent of their age JF - Front Bioeng Biotechnol N2 - Regeneration of bone defects is often limited due to compromised bone tissue physiology. Previous studies suggest that engineered extracellular matrices enhance the regenerative capacity of mesenchymal stromal cells. In this study, we used human-induced pluripotent stem cells, a scalable source of young mesenchymal progenitors (hiPSC-MPs), to generate extracellular matrix (iECM) and test its effects on the osteogenic capacity of human bone-marrow mesenchymal stromal cells (BMSCs). iECM was deposited as a layer on cell culture dishes and into three-dimensional (3D) silk-based spongy scaffolds. After decellularization, iECM maintained inherent structural proteins including collagens, fibronectin and laminin, and contained minimal residual DNA. Young adult and aged BMSCs cultured on the iECM layer in osteogenic medium exhibited a significant increase in proliferation, osteogenic marker expression, and mineralization as compared to tissue culture plastic. With BMSCs from aged donors, matrix mineralization was only detected when cultured on iECM, but not on tissue culture plastic. When cultured in 3D iECM/silk scaffolds, BMSCs exhibited significantly increased osteogenic gene expression levels and bone matrix deposition. iECM layer showed a similar enhancement of aged BMSC proliferation, osteogenic gene expression, and mineralization compared with extracellular matrix layers derived from young adult or aged BMSCs. However, iECM increased osteogenic differentiation and decreased adipocyte formation compared with single protein substrates including collagen and fibronectin. Together, our data suggest that the microenvironment comprised of iECM can enhance the osteogenic activity of BMSCs, providing a bioactive and scalable biomaterial strategy for enhancing bone regeneration in patients with delayed or failed bone healing. KW - aging KW - iPSCs KW - osteogenic differentiation KW - bone marrow stromal cells KW - extracellular matrix Y1 - U6 - http://dx.doi.org/https://doi.org/10.3389/fbioe.2023.1214019 VL - 11 ER - TY - JOUR A1 - Bernhard, Jonathan C A1 - Marolt Presen, Darja A1 - Li, Ming A1 - Monforte, Xavier A1 - Ferguson, James A1 - Leinfellner, Gabriele A1 - Heimel, Patrick A1 - Betti, Susanne L A1 - Shu, Sharon A1 - Teuschl-Woller, Andreas H A1 - Tangl, Stefan A1 - Redl, Heinz A1 - Vunjak-Novakovic, Gordana T1 - Effects of Endochondral and Intramembranous Ossification Pathways on Bone Tissue Formation and Vascularization in Human Tissue-Engineered Grafts JF - Cells N2 - Bone grafts can be engineered by differentiating human mesenchymal stromal cells (MSCs) via the endochondral and intramembranous ossification pathways. We evaluated the effects of each pathway on the properties of engineered bone grafts and their capacity to drive bone regeneration. Bone-marrow-derived MSCs were differentiated on silk scaffolds into either hypertrophic chondrocytes (hyper) or osteoblasts (osteo) over 5 weeks of in vitro cultivation, and were implanted subcutaneously for 12 weeks. The pathways' constructs were evaluated over time with respect to gene expression, composition, histomorphology, microstructure, vascularization and biomechanics. Hypertrophic chondrocytes expressed higher levels of osteogenic genes and deposited significantly more bone mineral and proteins than the osteoblasts. Before implantation, the mineral in the hyper group was less mature than that in the osteo group. Following 12 weeks of implantation, the hyper group had increased mineral density but a similar overall mineral composition compared with the osteo group. The hyper group also displayed significantly more blood vessel infiltration than the osteo group. Both groups contained M2 macrophages, indicating bone regeneration. These data suggest that, similar to the body's repair processes, endochondral pathway might be more advantageous when regenerating large defects, whereas intramembranous ossification could be utilized to guide the tissue formation pattern with a scaffold architecture. KW - bone tissue engineering KW - endochondral KW - mesenchymal stromal cells KW - ossification KW - intramembranous Y1 - U6 - http://dx.doi.org/10.3390/cells11193070 VL - 11 IS - 19:3070 ER - TY - JOUR A1 - Gollmann-Tepeköylü, Can A1 - Graber, Michael A1 - Hirsch, Jakob A1 - Mair, Sophia A1 - Naschberger, Andreas A1 - Pölzl, Leo A1 - Nägele, Felix A1 - Kirchmair, Elke A1 - Degenhart, Gerald A1 - Demetz, Egon A1 - Hilbe, Richard A1 - Chen, Hao-Yu A1 - Engert, James C A1 - Böhm, Anna A1 - Franz, Nadja A1 - Lobenwein, Daniela A1 - Lener, Daniela A1 - Fuchs, Christiane A1 - Weihs, Anna A1 - Töchterle, Sonja A1 - Vogel, Georg F A1 - Schweiger, Victor A1 - Eder, Jonas A1 - Pietschmann, Peter A1 - Seifert, Markus A1 - Kronenberg, Florian A1 - Coassin, Stefan A1 - Blumer, Michael A1 - Hackl, Hubert A1 - Meyer, Dirk A1 - Feuchtner, Gudrun A1 - Kirchmair, Rudolf A1 - Troppmair, Jakob A1 - Krane, Markus A1 - Weiss, Günther A1 - Tsimikas, Sotirios A1 - Thanassoulis, George A1 - Grimm, Michael A1 - Rupp, Bernhard A1 - Huber, Lukas A A1 - Zhang, Shen-Ying A1 - Casanova, Jean-Laurent A1 - Tancevski, Ivan A1 - Holfeld, Johannes T1 - Toll-Like Receptor 3 Mediates Aortic Stenosis Through a Conserved Mechanism of Calcification JF - Circulation KW - Toll-like receptor 3 KW - aortic valve disease KW - biglycan KW - extracellular matrix KW - osteogenesis Y1 - U6 - http://dx.doi.org/10.1161/CIRCULATIONAHA.122.063481 VL - 147 IS - 20 SP - 1518 EP - 1533 ER - TY - GEN A1 - Balz, Andrea A1 - Langer, Sarah A1 - Frohner, Matthias A1 - Forjan, Mathias T1 - Development of Internationalized Teaching and Training Modules for Healthcare Professionals T2 - Abstracts of the 2022 Joint Annual Conference of the Austrian (ÖGBMT), German (VDE DGBMT) and Swiss (SSBE) Societies for Biomedical Engineering, including the 14th Vienna International Workshop on Functional Electrical Stimulation KW - Internationalized Teaching KW - Healthcare Y1 - U6 - http://dx.doi.org/https://doi.org/10.1515/bmt-2022-2001 SP - 353 ER - TY - JOUR A1 - Xu, Yingyang A1 - Gepp, Barbara A1 - Lengger, Nina A1 - Yin, Jia A1 - Breiteneder, Heimo T1 - Identification of probable pectinesterase as a major allergen of pollen of the Asian white birch (Betula platyphylla) in northern China JF - Asian Pac J Allergy Immunol KW - birch pollen allergy KW - betula platyphylla KW - chinese population KW - major allergen KW - pectinesterase Y1 - U6 - http://dx.doi.org/10.12932/AP-100722-1409 ER - TY - GEN A1 - Eidi, Nada A1 - Balz, Andrea A1 - Forjan, Mathias T1 - MedTech-mR - Creating a Virtual Enviroment for Medical Training and Room Planning T2 - Abstracts of the 2022 Joint Annual Conference of the Austrian (ÖGBMT), German (VDE DGBMT) and Swiss (SSBE) Societies for Biomedical Engineering, including the 14th Vienna International Workshop on Functional Electrical Stimulation KW - Medical Training KW - Virtual Environment Y1 - U6 - http://dx.doi.org/https://doi.org/10.1515/bmt-2022-2001 SP - 349 ER -