TY - GEN A1 - Heher, Philipp A1 - Tomasch, Janine A1 - Maleiner, Babette A1 - Redl, Heinz A1 - Fuchs, Christiane T1 - The Importance of Biomechanical Cues for In Vitro Skeletal Myogenesis KW - In Vitro KW - Myogenesis Y1 - 2018 ER - TY - GEN A1 - Tomasch, Janine T1 - Generation of 3D skeletal muscle-like scaffolds via the application of mechanical stimuli KW - Scaffold KW - Mechanical Stimuli Y1 - 2018 ER - TY - CHAP A1 - Nebel, Sabrina A1 - Salado Manzano, Cristina A1 - Just, Valentin A1 - Leeb, Christine A1 - Jesenberger, Veronika T1 - Role of the MEK/ERK pathway in chondrogenic differentiation: Establishment of a protocol for the generation of MEK1-knockout hTERT ASCs and assessment of their differentiation potential T2 - Proceedings des Forschungsforum 2017 der österreichischen Fachhochschulen KW - Chondrogenesis Y1 - 2018 ER - TY - GEN A1 - Nebel, Sabrina A1 - Salado Manzano, Cristina A1 - Just, Valentin A1 - Leeb, Christine A1 - Jesenberger, Veronika T1 - Role of the MEK/ERK pathway in chondrogenic differentiation: Establishment of a protocol for the generation of MEK1-knockout hTERT ASCs and assessment of their differentiation potential KW - Chondrogenesis Y1 - 2018 ER - TY - GEN A1 - Gepp, Barbara A1 - Liousia, Varvara A1 - Machacek, Thomas A1 - Praher, Daniela A1 - Eisner, Katharina A1 - Limbeck, Sophie A1 - Gamper, Janine A1 - Schwarzl, Elisabeth A1 - Hazod, Till A1 - Landwehr, Renate A1 - Wenger, Charlotte A1 - Olscher, Christoph A1 - Leitner, Rita A1 - Rünzler, Dominik T1 - Pelargonic acid - an alternative to glyphosate-based herbicides? KW - Ecotoxicology KW - Herbicides Y1 - ER - TY - GEN A1 - Eisner, Katharina A1 - Gepp, Barbara A1 - Schnegg, Raimund A1 - Dallinger, Reinhard A1 - Rünzler, Dominik T1 - Investigation of endocrine effects of sublethal cadmium doses on the reproduction system of the freshwater snail B. glabrata KW - Ecotoxicology KW - Endocrine disruptors Y1 - ER - TY - JOUR A1 - Hackethal, Johannes A1 - Mühleder, Severin A1 - Hofer, Alexandra A1 - Schneider, Karl Heinrich A1 - Prüller, Johanna A1 - Hennerbichler, Simone A1 - Redl, Heinz A1 - Teuschl, Andreas T1 - An Effective Method of Atelocollagen Type 1/3 Isolation from Human Placenta and Its In Vitro Characterization in Two-Dimensional and Three-Dimensional Cell Culture Applications JF - Tissue Eng Part C Methods KW - Placenta KW - In Vitro KW - Cell Culture Y1 - 2018 VL - 23 IS - 5 SP - 274 EP - 285 ER - TY - CHAP A1 - Fuchs, Christiane A1 - Weihs, Anna A1 - Szwarc, Dorota A1 - Mittermayr, Rainer A1 - Rünzler, Dominik A1 - Teuschl, Andreas T1 - Shock wave treatment of muscle (stem) cells - a new implementation for regeneration T2 - Proceedings of the 20th International Congress of the ISMST KW - Shockwave treatment KW - Muscle Cells KW - Regeneration Y1 - 2018 ER - TY - JOUR A1 - Berkovitch, Yulia A1 - Cohen, Talia A1 - Peled, Eli A1 - Schmidhammer, Robert A1 - Hildner, Florian A1 - Teuschl, Andreas A1 - Wolbank, Susanne A1 - Yelin, Dvir A1 - Redl, Heinz A1 - Seliktar, Dror T1 - Hydrogel Composition and Laser Micro-Patterning to Regulate Sciatic Nerve Regeneration JF - Journal of Tissue Engineering and Regenerative Medicine KW - Micro-Patterning KW - Laser KW - Nerve Regeneration Y1 - 2018 VL - 12 IS - 4 ER - TY - GEN A1 - Fuchs, Christiane A1 - Weihs, Anna A1 - Szwarc, Dorota A1 - Mittermayr, Rainer A1 - Rünzler, Dominik A1 - Teuschl, Andreas T1 - Shock wave treatment of muscle (stem) cells - a new implementation for regeneration KW - Shockwave treatment KW - Muscle Cells KW - Regeneration Y1 - 2018 ER - TY - GEN A1 - Teuschl, Andreas A1 - Weihs, Anna A1 - Fuchs, Christiane A1 - Monforte Vila, Xavier T1 - Silk as a versatile biomaterial for musculoskeletal tissue engineering KW - Silk KW - Biomaterials Y1 - 2018 ER - TY - GEN A1 - Szwarc, Dorota A1 - Fuchs, Christiane A1 - Weihs, Anna A1 - Rünzler, Dominik T1 - Molecular mechanisms underlying the potential of shock wave treatment for cardiac therapy KW - Shockwave treatment KW - Cardiac Y1 - 2018 ER - TY - JOUR A1 - Bernhard, Jonathan A1 - Ferguson, James A1 - Rieder, Bernhard A1 - Heimel, Patrick A1 - Nau, Thomas A1 - Tangl, Stefan A1 - Redl, Heinz A1 - Vunjak-Novakovic, Gordana T1 - Tissue-engineered hypertrophic chondrocyte grafts enhanced long bone repair biomaterials JF - Biomaterials KW - Grafting KW - Biomaterials Y1 - 2018 IS - 139 SP - 202 EP - 212 ER - TY - GEN A1 - Rünzler, Dominik T1 - Alternatives to Animal Experiment Models: Tracking Zebrafish Larvae and Daphnia KW - Animal Experiments KW - Zebrafish Y1 - 2018 ER - TY - GEN A1 - Fuchs, Christiane T1 - The importance of mechanotransduction in myogenesis KW - Mechanotransduction KW - Myogenesis Y1 - 2018 ER - TY - JOUR A1 - Schneider, Cornelia A1 - Lehmann, Johannes A1 - van Osch, Gerjo A1 - Hildner, Florian A1 - Teuschl, Andreas A1 - Monforte Vila, Xavier A1 - Miosga, David A1 - Heimel, Patrick A1 - Priglinger, Eleni A1 - Redl, Heinz A1 - Wolbank, Susanne A1 - Nürnberger, Sylvia T1 - Systematic Comparison of Protocols for the Preparation of Human Articular Cartilage for Use as Scaffold Material in Cartilage Tissue Engineering JF - Tissue Eng Part C Methods KW - Cartilage KW - Scaffold Material Y1 - 2018 VL - 22 IS - 12 ER - TY - CHAP A1 - Maleiner, Babette A1 - Heher, Philipp A1 - Teuschl, Andreas A1 - Redl, Heinz A1 - Rünzler, Dominik A1 - Fuchs, Christiane T1 - Generation of aligned skeletal muscle-like tissue based on the application of strain to a 3D fibrin scaffold T2 - Proceedings of PACT "Designer Cells go Clinical" Symposium KW - Tissue Generation KW - Fibrin KW - Scaffold Y1 - 2018 ER - TY - GEN A1 - Szwarc, Dorota A1 - Fuchs, Christiane A1 - Weihs, Anna A1 - Monforte Vila, Xavier A1 - Hanetseder, Dominik A1 - Teuschl, Andreas A1 - Rünzler, Dominik T1 - The effect of shock waves on in vitro cartilage development in silk scaffolds KW - Shockwave treatment KW - In Vitro KW - Cartilage KW - Silk Y1 - 2018 ER - TY - GEN A1 - Maleiner, Babette A1 - Heher, Philipp A1 - Teuschl, Andreas A1 - Redl, Heinz A1 - Rünzler, Dominik A1 - Fuchs, Christiane T1 - Generation of aligned skeletal muscle-like tissue based on the application of strain to a 3D fibrin scaffold KW - Tissue Generation KW - Fibrin KW - Scaffold Y1 - 2018 ER - TY - GEN A1 - Teuschl, Andreas A1 - Fuchs, Christiane A1 - Weihs, Anna A1 - Heimel, Patrick A1 - Rünzler, Dominik A1 - Redl, Heinz A1 - Nau, Thomas T1 - The Silk Road from Textiles to Novel Medical Implants KW - Silk Y1 - 2018 ER - TY - GEN A1 - Teuschl, Andreas A1 - Heimel, Patrick A1 - Nürnberger, Sylvia A1 - Redl, Heinz A1 - Nau, Thomas T1 - ACL Regeneration using a novel silk fiber based scaffold - Histological Results of a Large Animal Study KW - Tissue Regeneration KW - Silk KW - Scaffold Y1 - 2018 ER - TY - GEN A1 - Teuschl, Andreas T1 - Silk Processing - from Gels to ACL Replacement KW - Silk Y1 - 2018 ER - TY - CHAP A1 - Fuchs, Christiane A1 - Szwarc, Dorota A1 - Weihs, Anna A1 - Rünzler, Dominik T1 - Shock wave treatment of 3D cardiac model systems activates ERK 1/2 signaling pathway and influences cardiomyogenesis T2 - Proceedings of PACT "Designer Cells go Clinical" Symposium KW - Shockwave treatment KW - Cardiac Y1 - 2018 ER - TY - CHAP A1 - Fuchs, Christiane A1 - Szwarc, Dorota A1 - Weihs, Anna A1 - Rünzler, Dominik T1 - Shock wave treatment of 3D cardiac model systems activates ERK 1/2 signaling pathway and influences cardiomyogenesis T2 - Proceedings of LBG Meeting for Health Sciences 2016 KW - Shockwave treatment KW - Cardiac Y1 - 2018 ER - TY - GEN A1 - Fuchs, Christiane A1 - Szwarc, Dorota A1 - Weihs, Anna A1 - Rünzler, Dominik T1 - Shock wave treatment of 3D cardiac model systems activates ERK 1/2 signaling pathway and influences cardiomyogenesis KW - Shockwave treatment KW - Cardiac Y1 - 2018 ER - TY - CHAP A1 - Fuchs, Christiane A1 - Szwarc, Dorota A1 - Weihs, Anna A1 - Rünzler, Dominik T1 - Shock wave treatment positively influences cardiomyogenesis in an energy-dependent manner T2 - Proceedings of PACT "Designer Cells go Clinical" Symposium KW - Shockwave treatment KW - Cardiac Y1 - 2018 ER - TY - GEN A1 - Weihs, Anna T1 - Shock wave treatment for in vitro tissue engineering applications KW - Shockwave treatment KW - In Vitro KW - Tissue Engineering Y1 - 2018 ER - TY - CHAP A1 - Basoli, Valentina A1 - Chaudry, Sidrah A1 - Cruciani, Sara A1 - Fuchs, Christiane A1 - Rieger, Sabine A1 - Dungel, Peter A1 - Wolbank, Susanne A1 - Ventura, Carlo A1 - Grillari-Voglauer, Regina A1 - Redl, Heinz A1 - Maioli, Margherita T1 - Epigenetic and molecular behavious of stem cells exposed to biophysical stimuli: new insights in regenerative medicine T2 - Proceedings des Seminars zum 40. Jahresjubiläum der Österreichischen Gesellschaft für Chirurgische Forschung KW - Stem Cells KW - Regeneration KW - Biophysical Stimuli Y1 - 2018 ER - TY - GEN A1 - Basoli, Valentina A1 - Chaudry, Sidrah A1 - Cruciani, Gabriele A1 - Fuchs, Christiane A1 - Rieger, Sabine A1 - Dungel, Peter A1 - Wolbank, Susanne A1 - Ventura, Carlo A1 - Grillari-Voglauer, Regina A1 - Redl, Heinz A1 - Maioli, Margherita T1 - Epigenetic and molecular behavious of stem cells exposed to biophysical stimuli: new insights in regenerative medicine KW - Stem Cells KW - Regeneration KW - Biophysical Stimuli Y1 - 2018 ER - TY - GEN A1 - Weihs, Anna T1 - Shock wave treatment for in vitro tissue engineering applications KW - Shockwave treatment KW - In Vitro KW - Tissue Engineering Y1 - 2018 ER - TY - GEN A1 - Weihs, Anna T1 - Mechanotransduction in shock wave treatment KW - Shockwave treatment KW - Mechanotransduction Y1 - 2018 ER - TY - GEN A1 - Mandt, Denise A1 - Pichler, Verena A1 - Melchior, Sophie A1 - Rünzler, Dominik T1 - Establishment of 2D and 3D cell culture assays using RT-gillW1 cells KW - Cell Culture KW - Assay Y1 - 2018 ER - TY - GEN A1 - Salzer, Elias A1 - Rünzler, Dominik T1 - Acute Immobilization Test with Daphnia magna - A Refined Methodology for the Detection of Immobilization KW - Immobilization Y1 - 2018 ER - TY - GEN A1 - Salzer, Elias A1 - Rünzler, Dominik T1 - Live or dead is not enough: a large-scale mobility assay with Daphnia magna KW - Mobility Assay Y1 - 2018 ER - TY - GEN A1 - Fuchs, Christiane T1 - A mechanical stimulation strategy for engineering skeletal muscle-like tissue on a strained fibrin scaffold KW - Muscle Tissue KW - Fibrin KW - Scaffold Y1 - 2018 ER - TY - GEN A1 - Fuchs, Christiane T1 - The beneficial effects of in vitro shock wave treatment on cardiomyogenesis are energy dependent KW - In Vitro KW - Shockwave treatment KW - Myogenesis Y1 - 2018 ER - TY - GEN A1 - Fuchs, Christiane T1 - Mechanotransduction - bioreactors KW - Bioreactors Y1 - 2018 ER - TY - GEN A1 - Teuschl, Andreas A1 - Schuh, Christina A1 - Halbweis, Robert A1 - Marton, Gábor A1 - Pajer, Krisztián A1 - Hopf, Rudolf A1 - Mosia, Shorena A1 - Rünzler, Dominik A1 - Nógrádi, Antal A1 - Hausner, Thomas A1 - Redl, Heinz T1 - Silk fibroin for peripheral nerve regeneration: a novel preparation method improved mechanical characteristics and supports regeneration in rat sciatic nerves KW - Fibroin KW - Regeneration Y1 - 2018 ER - TY - GEN A1 - Fuchs, Christiane A1 - Weihs, Anna A1 - Teuschl, Andreas A1 - Hartinger, Joachim A1 - Slezak, Paul A1 - Mittermayr, Rainer A1 - Redl, Heinz A1 - Junger, Wolfgang A1 - Sitte, Harald A1 - Rünzler, Dominik T1 - Shockwave Treatment Enhances Proliferation and Improves Wound Healing via Purinergic Signaling Linked ERK 1/2 Pathways KW - Shockwave treatment KW - Healing Processes KW - Signaling Y1 - 2018 ER - TY - GEN A1 - Fuchs, Christiane A1 - Weihs, Anna A1 - Teuschl, Andreas A1 - Hartinger, Joachim A1 - Slezak, Paul A1 - Mittermayr, Rainer A1 - Redl, Heinz A1 - Junger, Wolfgang A1 - Sitte, Harald A1 - Rünzler, Dominik T1 - Shockwave Treatment Augments Proliferation and Improves Wound Healing via Purinergic Signaling Linked ERK 1/2 Pathways KW - Shockwave treatment KW - Healing Processes KW - Signaling Y1 - 2018 ER - TY - GEN A1 - Teuschl, Andreas A1 - Fuchs, Christiane A1 - Feichtinger, Georg A1 - Heher, Philipp A1 - Heimel, Patrick A1 - Schuh, Christina A1 - Nürnberger, Sylvia A1 - Nau, Thomas A1 - Rünzler, Dominik A1 - Redl, Heinz T1 - Fibrin or Fibroin - Not only the "o" Makes the Difference KW - Fibrin KW - Fibroin Y1 - 2018 ER - TY - JOUR A1 - Teuschl, Andreas A1 - Balmayor, Elizabeth A1 - Redl, Heinz A1 - van Griensven, Martijn A1 - Dungel, Peter T1 - Phototherapy With LED Light Modulates Healing Processes in an In Vitro Scratch Wound-Model Using 3 Different Cell Types JF - Dermatologic Surgery KW - Phototherapy KW - Healing Processes Y1 - VL - 41 IS - 2 SP - 261 EP - 268 ER - TY - GEN A1 - Teuschl, Andreas A1 - Nürnberger, Sylvia A1 - Heimel, Patrick A1 - Redl, Heinz A1 - Nau, Thomas T1 - Regeneration of the Anterior Cruciate Ligament Using a Silk-Fiber Based Scaffold - Histological Results KW - Tissue Regeneration KW - Ligaments KW - Scaffold Y1 - 2018 ER - TY - JOUR A1 - Nau, Thomas A1 - Teuschl, Andreas T1 - Regeneration of the anterior cruciate ligament: Current strategies in tissue engineering JF - World Journal of Orthopedics KW - Ligament KW - Regeneration KW - Tissue Engineering Y1 - VL - 6 IS - 1 ER - TY - THES A1 - Weihs, Anna T1 - Elucidation of extracorporeal shock wave treatment triggered intracellular processes N2 - Die Wirkung von Stoßwellen auf den menschlichen Körper ist bereits seit Jahrzehnten bekannt. Ihr zerstörerisches Potential wird seit den 1980-ern zur Behandlung und Desintegration von Nierensteinen angewendet, wo die Stoßwellentherapie heutzutage die Standardbehandlung darstellt. Der erste unerwartete Effekt der Therapie - die Verdichtung des Beckenkammes - wurde schon damals bei Folgeuntersuchungen von Patienten mit Nierensteinen festgestellt. Die Beobachtung, dass Stoßwellen Effekte auf Knochen hervorrufen, ebnete den Weg für die Anwendung der Stoßwellentherapie in Bereichen abseits der Urologie. Mittlerweile wird die Stoßwellentherapie nicht nur zur Behandlung von Knochenbruchheilungsstörungen und Tendinopathien, sondern auch bei Weichteilwundheilungsstörungen eingesetzt. Trotz der vielfältigen Anwendungsgebiete ist der zugrunde liegende Wirkungsmechanismus des positiven Effekts der Stoßwellentherapie bis heute noch nicht vollständig aufgeklärt. Die Aktivierung von mechanotransduktiven Signalwegen wurde bereits teilweise in vitro und in vivo gezeigt, jedoch meist bei knöchernen Indikationen. Doch auch für die Anwendung der Stoßwellentherapie in der (verzögerten) Wundheilung ist die Aufklärung des Wirkungsmechanismus essentiell. Erst dadurch könnte diese nicht- invasive, effiziente und großteils Nebenwirkungs-freie Therapieform auch in diesem Bereich als mögliche Standardtherapieform genutzt werden. In dieser Dissertation wurde zuerst ein Set-up zur in vitro Stoßwellenanwendung optimiert. Mithilfe eines Molekül-Aufnahme Assays wurden die technischen Parameter für die in vitro Stoßwellenbehandlung festgelegt. Mit diesem in vitro Aufbau sollten daraufhin jene intrazellulären Mechanismen identifiziert werden, die von der Stoßwelle beeinflusst werden. In einem in vivo Modell sollte schlussendlich die Rolle dieser Mechanismen am wundheilungsfördernden Effekt der Stoßwelle geklärt werden. Um möglichst universelle Effekte der Stoßwelle auf intrazelluläre Signalwege zu untersuchen, wurden verschiedenste Zelllinien verwendet. Diese beinhalteten die humane U937 Monozyten Zelllinie, humane Jurkat T-Zellen, die humane MG63 Osteosarcoma Zelllinie, die murine C3H10T1/2 mesenchymale Progenitor Zelllinie sowie primäre humane mononukleäre Zellen des peripheren Blutes. Die in den proliferativen Effekt der Stowellentherapie involvierte Signalkaskade konnte darauffolgend erstmals sowohl in murinen C3H10T1/2 Zellen als auch in humanen Fettstammzellen (adipose tissue-derived stem cells) und in humanen Jurkat T-Zellen detailliert beschrieben werden. Außerdem wurde ATP als entscheidendes Signalmolekül identifiziert, welches nach Freisetzung durch Stoßwellenbehandlung mittels purinergem Signaling die Erk1/2 Signalkaskade aktiviert. Für die dadurch gesteigerte Proliferation in stoßwellenbehandelten Zellen ist die Erk1/2 Aktivierung essentiell. In einem in vivo Model für gestörte Wundheilung in der Ratte wurde die Hypothese zur entscheidenden Rolle des Erk1/2 Signalweges im wundheilungsfördernden Effekt der Stoßwelle bestätigt. In dieser Dissertation wurde gezeigt, dass die durch purinerges Signaling aktivierte Erk1/2 Signalkaskade eine entscheidende Rolle in der durch Stoßwellentherapie beschleunigten Zellproliferation in vitro und Wundheilung in vivo übernimmt. Das damit erweiterte Verständnis der Wirkungsmechanismen der Stoßwellentherapie kann zur Weiterentwicklung dieser Behandlungsform als zukünftige Standardtherapie bei Wundheilungsstörungen, wie z.B. für diabetische oder chronische Wunden, beitragen. N2 - Since the early 1980s, shock wave treatment has been the golden standard treatment option for the disintegration of kidney stones in urology. A wide range of beneficial effects of shock waves on the human body was soon identified, starting with first observations of bone densification at the iliac crest after treatment of kidney stones. Since then, the indications for shock wave therapy have conquered areas apart from the field of urology. Nowadays, shock wave therapy is used for a variety of indications such as tendinopathies or impaired bone healing. Furthermore, patients suffering from poor wound healing such as diabetic foot ulcers and also chronic, non-healing wounds are treated successfully with shock waves. Despite the versatile application fields of shock wave therapy, the general principles underlying the beneficial effect of this treatment still remain to be fully elucidated. Several in vitro and in vivo studies, mostly involving osteoblast like cells and the osteo-inductive potential of shock wave treatment, already highlighted the role of the activation of mechanotransductory signaling pathways. For the clinical application of shock wave therapy as an accepted treatment for critically healing wounds (e.g. chronic or diabetic wounds, burns), general mechanistic evidence to explain the underlying mechanisms is essential. These data would facilitate the standardized application of this non-invasive, cost efficient and low- risk bearing therapy, which can be performed in an outpatient setting. First of all, an in vitro set-up was established and the necessary technical parameters for the optimal application of shock wave treatment on cell cultures were defined in this thesis. For this purpose, a molecule uptake assay was used as a functional assay. The following aims of this study were to elucidate the effect of shock wave treatment on intracellular signaling in vitro and to ultimately describe their role in the wound healing effect of shock wave treatment in vivo. To identify universal effects of shock wave treatment on intracellular signaling mechanisms, various cell lines were used, including the human U937 monocytic cell line, a human Jurkat T-cell line, the human MG63 osteosarcoma cell line, the C3H10T1/2 mouse mesenchymal progenitor cell line as well as primary human peripheral mononuclear cells. For the first time, the affected signaling cascade leading to the proliferative effect of shock wave treatment in vitro was described in detail in mouse C3H10T1/2 cells as well as in human adipose tissue-derived stem cells and human Jurkat T-cells. Further, ATP release from shock wave treated cells was shown to initiate intracellular Erk1/2 signaling activation via purinergic signaling. The thereby ultimately increased proliferation was reported to be dependent on shock wave treatment triggered Erk1/2 pathway activation. An in vivo study on impaired wound healing in rats confirmed the hypothesis on the essential role of Erk1/2 signaling in the shock wave treatment induced wound healing effect. Data clearly indicate the crucial importance of the Erk1/2 signaling cascade in the proliferative and wound healing effect of shock wave treatment. Conclusively, purinergic signaling activated Erk1/2 signaling cascades play an essential role in the shock wave treatment induced proliferative and wound healing effect. The thereby broadened knowledge on the underlying mechanistic principles of the effect of shock wave treatment contributes to the establishment of shock wave therapy as a feasible standard treatment for soft tissue wound healing disorders such as diabetic or chronic wounds. KW - Healing KW - Shockwave Y1 - 2018 ER - TY - JOUR A1 - Rosser, Julie A1 - Calvo, Isabel Olmos A1 - Schlager, Magdalena A1 - Purtscher, Michaela A1 - Jenner, Florien A1 - Ertl, Peter T1 - Recent Advances of Biologically Inspired 3D Microfluidic Hydrogel Cell Culture Systems JF - J Cell Biol Cell Metalab KW - Hydrogel KW - Cell Culture Y1 - IS - 2 ER - TY - GEN A1 - Kroller, Theophil A1 - Kreuzinger, Norbert A1 - Rünzler, Dominik T1 - Validierung und Anwendung eines zellkultur-basierten Assays für Screening von endokrin wirksamen Stoffen in Abwasser KW - Assay KW - Sewage Water Y1 - 2018 ER - TY - GEN A1 - Guillaume, Olivier A1 - Monforte Vila, Xavier T1 - Development of a Biodegradable Silk Mesh Suitable for Intra-Operative Fast Cell Seeding KW - Silk KW - Cell Seeding Y1 - 2018 ER - TY - GEN A1 - Heher, Philipp A1 - Fuchs, Christiane A1 - Prüller, Johanna A1 - Maleiner, Babette A1 - Kollmitzer, Josef A1 - Rünzler, Dominik A1 - Teuschl, Andreas A1 - Wolbank, Susanne A1 - Redl, Heinz T1 - A bioreactor-based 3D culture system for skeletal muscle engineering in fibrin scaffolds KW - Bioreactors KW - Cell Culture KW - Muscle KW - Scaffold Y1 - 2018 ER - TY - JOUR A1 - Banerjee, Asmita A1 - Nürnberger, Sylvia A1 - Hennerbichler, Simone A1 - Riedl, Stefan A1 - Schuh, Christina A1 - Hacobian, Ara A1 - Teuschl, Andreas A1 - Eibl, Jürgen A1 - Redl, Heinz T1 - In toto differentiation of human amniotic membrane towards the Schwann cell lineage JF - 227-239 KW - Membrane KW - In toto differentiation Y1 - 2018 VL - 15 IS - 2 ER - TY - JOUR A1 - Buta, Christiane A1 - David, Robert A1 - Dressel, Ralf A1 - Emgard, Mia A1 - Fuchs, Christiane A1 - Gross, Ulrike A1 - Healy, Lyn A1 - Hescheler, Jürgen A1 - Kolar, Roman A1 - Martin, Ulrich A1 - Mikkers, Harald A1 - Müller, Franz-Josef A1 - Schneider, Rebekka A1 - Seiler, Andrea A1 - Spielmann, Horst A1 - Weitzer, Georg T1 - REconsidering pluripotency tests: do we still need teratoma assays? JF - Stem Cell Research KW - Assay KW - Stem Cells Y1 - 2018 VL - 11 IS - 1 SP - 552 EP - 562 ER - TY - JOUR A1 - Hohlrieder, Manfred A1 - Teuschl, Andreas A1 - Cicha, Klaus A1 - van Griensven, Martijn A1 - Redl, Heinz A1 - Stampfl, Jürgen T1 - Bioreactor and scaffold design for the mechanical stimulation of anterior cruciate ligament grafts JF - Biomedical materials and engineering KW - Bioreactors KW - Ligaments KW - Grafting Y1 - 2018 VL - 23 IS - 3 SP - 225 EP - 237 ER - TY - JOUR A1 - Dungel, Peter A1 - Teuschl, Andreas A1 - Banerjee, Asmita A1 - Paier-Pourani, Jamile A1 - Redl, Heinz A1 - Kozlov, Andrey T1 - Impact of mitochondria on nitrite metabolism in HL-1 cardiomyocytes JF - Frontiers in Physiology KW - Nitrite KW - Metabolism Y1 - 2018 IS - 4 ER - TY - JOUR A1 - Teuschl, Andreas A1 - Nürnberger, Sylvia A1 - Redl, Heinz A1 - Nau, Thomas T1 - Articular cartilage tissue regeneration: current research strategies and outlook for the future JF - European Surgery KW - Tissue Regeneration KW - Cartilage Tissue Y1 - 2018 VL - 45 IS - 3 SP - 142 EP - 153 ER - TY - JOUR A1 - Teuschl, Andreas A1 - van Griensven, Martijn A1 - Redl, Heinz T1 - Sericin removal from raw Bombys mori silk scaffolds of high hierarchical order JF - Tissue Eng Part C Methods KW - Scaffold KW - Sericin Y1 - 2018 ER - TY - GEN A1 - Kamravamanesh, Donya A1 - Pflügl, Stefan A1 - Lackner, Maximilian A1 - Herwig, Christoph T1 - Enhancing PHB Production in Synechocystis sp. PCC 6714 by means of Process Engineering and Multivariate Design of Experiments KW - PHB KW - Bacteria KW - Process Engineering Y1 - 2018 ER - TY - GEN A1 - Purtscher, Michaela A1 - Ergir, Ece A1 - Szwarc, Dorota A1 - Monforte Vila, Xavier A1 - Rünzler, Dominik A1 - Huber-Gries, Carina T1 - A microfluidic-based easy-to-use cardiac tissue model for drug screening applications KW - Tissue Generation KW - Drug Screening KW - Cardiac Y1 - 2018 ER - TY - GEN A1 - Purtscher, Michaela A1 - Ergir, Ece A1 - Monforte Vila, Xavier A1 - Huber-Gries, Carina T1 - Establishment of an in vitro heart tissue model for pre-clinical screening of therapeutic agents using microfluidic technology KW - Tissue Generation KW - Heart Tissue Y1 - 2018 ER - TY - GEN A1 - Purtscher, Michaela A1 - Ergir, Ece A1 - Szwarc, Dorota A1 - Huber-Gries, Carina T1 - Microfluidic based heart-tissue model for directed development of cardiac specific cell types KW - Heart Tissue KW - Tissue Generation KW - Cardiac Y1 - 2018 ER - TY - GEN A1 - Lackner, Maximilian T1 - Cyanobacteria as model organism for PHB production KW - Bacteria KW - PHB Y1 - 2018 ER - TY - GEN A1 - Lackner, Maximilian A1 - Kamravamanesh, Donya A1 - Markl, Erich A1 - Engelhardt-Nowitzki, Corinna A1 - Herwig, Christoph T1 - Optimization of process parameters to enhance Polyhydroxyalkanoate accumulation in a unicellular cyanobacterium using multivariate experimental design approach KW - Bacteria Y1 - 2018 ER - TY - JOUR A1 - Teuschl, Andreas A1 - Zipperle, Johannes A1 - Huber-Gries, Carina A1 - Kaplan, David T1 - Silk fibroin based carrier system for delivery of fibrinogen and thrombin as coagulant supplements JF - Journal of Biomedical Materials Research KW - Fibrin KW - Silk Y1 - 2018 VL - 105 IS - 3 ER - TY - GEN A1 - Kamravamanesh, Donya A1 - Pflügl, Stefan A1 - Lackner, Maximilian A1 - Herwig, Christoph T1 - Photosynthetic poly-β-hydroxybutyrate accumulation in unicellular cyanobacterium Synechocystis sp. PCC 6714 KW - Bacteria Y1 - 2018 ER - TY - CHAP A1 - Leeb, Christine A1 - Leitner, Rita A1 - Pichler, Verena A1 - Huber-Gries, Carina A1 - Rünzler, Dominik A1 - Jesenberger, Veronika T1 - Einführung und Optimierung eines praxisorientierten PBL-Moduls im Life-Science-Bereich T2 - Proceedings des Kongresses Problem-Based Learning 2016 PBL - Kompetenzen fördern, Zukunft gestalten KW - Life Science KW - Problem Based Learning KW - Education Y1 - 2018 ER - TY - JOUR A1 - Kamravamanesh, Donya A1 - Pflügl, Stefan A1 - Nischkauer, Winfried A1 - Limbeck, Andreas A1 - Lackner, Maximilian A1 - Herwig, Christoph T1 - Photosynthetic poly-β-hydroxybutyrate accumulation in unicellular cyanobacterium Synechocystis sp. PCC 6714 JF - AMB Express KW - Bacteria Y1 - 2018 VL - 143 IS - 7 ER - TY - GEN A1 - Kamravamanesh, Donya A1 - Pflügl, Stefan A1 - Lackner, Maximilian A1 - Herwig, Christoph T1 - Photosynthetic poly-β-hydroxybutyrate accumulation in unicellular cyanobacterium Synechocystis sp. PCC 6714 KW - Bacteria Y1 - 2018 ER - TY - BOOK A1 - Lackner, Maximilian A1 - Grabow, Wilhelm A1 - Stadler, Philipp T1 - Handbook of Online and Near-real-time Methods in Microbiology KW - Microbiology Y1 - 2018 ER - TY - GEN A1 - Lackner, Maximilian T1 - PBAT - A versatile bioplastics KW - Bioplastics Y1 - 2018 ER - TY - GEN A1 - Mühleder, Severin A1 - Fuchs, Christiane A1 - Bassilio, Jose A1 - Sczwarc, Dorota A1 - Pill, Karoline A1 - Slezak, Paul A1 - Labuda, Krystina A1 - Siehs, Christian A1 - Pröll, Johannes A1 - Priglinger, Eleni A1 - Redl, Heinz A1 - Holnthoner, Wolfgang T1 - The purinergic receptor P2Y2 modulates endothelial sprouting and angiogenesis KW - Angiogenesis Y1 - 2018 ER - TY - JOUR A1 - Bachmann, Barbara A1 - Spitz, Sarah A1 - Rothbauer, Mario A1 - Jordan, Christian A1 - Purtscher, Michaela A1 - Zirath, Helene A1 - Schuller, Patrick A1 - Eilenberger, Christoph A1 - Ali, Syed Faheem A1 - Mühleder, Severin A1 - Priglinger, Eleni A1 - Harasek, Michael A1 - Redl, Heinz A1 - Holnthoner, Wolfgang A1 - Ertl, Peter T1 - Engineering of three-dimensional pre-vascular networks within fibrin hydrogel constructs by microfluidic control over reciprocal cell signaling JF - Biomicrofluidics KW - Microfluidic KW - Vascularization KW - Tissue Engineering Y1 - 2019 ER - TY - GEN A1 - Schneider, Karl Heinrich A1 - Aigner, Petra A1 - Monforte Vila, Xavier A1 - Holnthoner, Wolfgang A1 - Teuschl, Andreas A1 - Bergmeister, Helga A1 - Redl, Heinz T1 - Naturally derived acellular small diameter vascular grafts from human placenta for reconstructive surgery KW - Placenta KW - Grafting KW - Surgery Y1 - 2018 ER - TY - GEN A1 - Liousia, Varvara A1 - Salzer, Elias A1 - Mandt, Denise A1 - Rünzler, Dominik T1 - The impact of ethanol in vitro and in vivo: a comparative study KW - In Vivo KW - In Vitro KW - Ethanol Y1 - 2018 ER - TY - GEN A1 - Liousia, Varvara A1 - Rünzler, Dominik T1 - Stage- and dose-dependent effects of methanol and ethanol on the locomotor activity of zebrafish larvae KW - Locomotor Activity KW - Zebrafish Y1 - 2018 ER - TY - JOUR A1 - Kierspel, Thomas A1 - Kadek, Alan A1 - Barran, Perdita A1 - Bellina, Bruno A1 - Bijedic, Adi A1 - Brodmerkel, Maxim N. A1 - Commandeur, Jan A1 - Caleman, Carl A1 - Damjanovic, Tomislav A1 - Dawod, Ibrahim A1 - De Santis, Emiliano A1 - Lekkas, Alexandros A1 - Lorenzen, Kristina A1 - López Morillo, Luis A1 - Mandl, Thomas A1 - Marklund, Erik G. A1 - Papanastasiou, Dimitris A1 - Ramakers, Lennart A. I. A1 - Schweikhard, Lutz A1 - Simke, Florian A1 - Sinelnikova, Anna A1 - Smyrnakis, Athanasios A1 - Timneanu, Nicusor A1 - Uetrecht, Charlotte T1 - Coherent diffractive imaging of proteins and viral capsids: simulating MS SPIDOC JF - Analytical and Bioanalytical Chemistry N2 - MS SPIDOC is a novel sample delivery system designed for single (isolated) particle imaging at X-ray Free-Electron Lasers that is adaptable towards most large-scale facility beamlines. Biological samples can range from small proteins to MDa particles. Following nano-electrospray ionization, ionic samples can be m/z-filtered and structurally separated before being oriented at the interaction zone. Here, we present the simulation package developed alongside this prototype. The first part describes how the front-to-end ion trajectory simulations have been conducted. Highlighted is a quadrant lens; a simple but efficient device that steers the ion beam within the vicinity of the strong DC orientation field in the interaction zone to ensure spatial overlap with the X-rays. The second part focuses on protein orientation and discusses its potential with respect to diffractive imaging methods. Last, coherent diffractive imaging of prototypical T = 1 and T = 3 norovirus capsids is shown. We use realistic experimental parameters from the SPB/SFX instrument at the European XFEL to demonstrate that low- resolution diffractive imaging data (q < 0.3 nm −1 ) can be collected with only a few X-ray pulses. Such low-resolution data are sufficient to distinguish between both symmetries of the capsids, allowing to probe low abundant species in a beam if MS SPIDOC is used as sample delivery. KW - SPI KW - X-ray KW - Native MS KW - Protein complex structure KW - Simulation Y1 - U6 - http://dx.doi.org/https://doi.org/10.1007/s00216-023-04658-y VL - 2023 IS - 415 SP - 4209 EP - 4220 ER -