TY  - JOUR
A1  - Bernhard, Jonathan C
A1  - Marolt Presen, Darja
A1  - Li, Ming
A1  - Monforte, Xavier
A1  - Ferguson, James
A1  - Leinfellner, Gabriele
A1  - Heimel, Patrick
A1  - Betti, Susanne L
A1  - Shu, Sharon
A1  - Teuschl-Woller, Andreas H
A1  - Tangl, Stefan
A1  - Redl, Heinz
A1  - Vunjak-Novakovic, Gordana
T1  - Effects of Endochondral and Intramembranous Ossification Pathways on Bone Tissue Formation and Vascularization in Human Tissue-Engineered Grafts
JF  - Cells
N2  - Bone grafts can be engineered by differentiating human mesenchymal stromal cells (MSCs) via the endochondral and intramembranous ossification pathways. We evaluated the effects of each pathway on the properties of engineered bone grafts and their capacity to drive bone regeneration. Bone-marrow-derived MSCs were differentiated on silk scaffolds into either hypertrophic chondrocytes (hyper) or osteoblasts (osteo) over 5 weeks of in vitro cultivation, and were implanted subcutaneously for 12 weeks. The pathways' constructs were evaluated over time with respect to gene expression, composition, histomorphology, microstructure, vascularization and biomechanics. Hypertrophic chondrocytes expressed higher levels of osteogenic genes and deposited significantly more bone mineral and proteins than the osteoblasts. Before implantation, the mineral in the hyper group was less mature than that in the osteo group. Following 12 weeks of implantation, the hyper group had increased mineral density but a similar overall mineral composition compared with the osteo group. The hyper group also displayed significantly more blood vessel infiltration than the osteo group. Both groups contained M2 macrophages, indicating bone regeneration. These data suggest that, similar to the body's repair processes, endochondral pathway might be more advantageous when regenerating large defects, whereas intramembranous ossification could be utilized to guide the tissue formation pattern with a scaffold architecture.
KW  - bone tissue engineering
KW  - endochondral
KW  - mesenchymal stromal cells
KW  - ossification
KW  - intramembranous
Y1  - 
U6  - http://dx.doi.org/10.3390/cells11193070
VL  - 11
IS  - 19:3070
ER  - 
TY  - JOUR
A1  - Gollmann-Tepeköylü, Can
A1  - Graber, Michael
A1  - Hirsch, Jakob
A1  - Mair, Sophia
A1  - Naschberger, Andreas
A1  - Pölzl, Leo
A1  - Nägele, Felix
A1  - Kirchmair, Elke
A1  - Degenhart, Gerald
A1  - Demetz, Egon
A1  - Hilbe, Richard
A1  - Chen, Hao-Yu
A1  - Engert, James C
A1  - Böhm, Anna
A1  - Franz, Nadja
A1  - Lobenwein, Daniela
A1  - Lener, Daniela
A1  - Fuchs, Christiane
A1  - Weihs, Anna
A1  - Töchterle, Sonja
A1  - Vogel, Georg F
A1  - Schweiger, Victor
A1  - Eder, Jonas
A1  - Pietschmann, Peter
A1  - Seifert, Markus
A1  - Kronenberg, Florian
A1  - Coassin, Stefan
A1  - Blumer, Michael
A1  - Hackl, Hubert
A1  - Meyer, Dirk
A1  - Feuchtner, Gudrun
A1  - Kirchmair, Rudolf
A1  - Troppmair, Jakob
A1  - Krane, Markus
A1  - Weiss, Günther
A1  - Tsimikas, Sotirios
A1  - Thanassoulis, George
A1  - Grimm, Michael
A1  - Rupp, Bernhard
A1  - Huber, Lukas A
A1  - Zhang, Shen-Ying
A1  - Casanova, Jean-Laurent
A1  - Tancevski, Ivan
A1  - Holfeld, Johannes
T1  - Toll-Like Receptor 3 Mediates Aortic Stenosis Through a Conserved Mechanism of Calcification
JF  - Circulation
KW  - Toll-like receptor 3
KW  - aortic valve disease
KW  - biglycan
KW  - extracellular matrix
KW  - osteogenesis
Y1  - 
U6  - http://dx.doi.org/10.1161/CIRCULATIONAHA.122.063481
VL  - 147
IS  - 20
SP  - 1518
EP  - 1533
ER  - 
TY  - GEN
A1  - Balz, Andrea
A1  - Langer, Sarah
A1  - Frohner, Matthias
A1  - Forjan, Mathias
T1  - Development of Internationalized Teaching and Training Modules for Healthcare Professionals
T2  - Abstracts of the 2022 Joint Annual Conference of the Austrian (ÖGBMT), German (VDE DGBMT) and Swiss (SSBE) Societies for Biomedical Engineering, including the 14th Vienna International Workshop on Functional Electrical Stimulation
KW  - Internationalized Teaching
KW  - Healthcare
Y1  - 
U6  - http://dx.doi.org/https://doi.org/10.1515/bmt-2022-2001
SP  - 353
ER  - 
TY  - JOUR
A1  - Xu, Yingyang
A1  - Gepp, Barbara
A1  - Lengger, Nina
A1  - Yin, Jia
A1  - Breiteneder, Heimo
T1  - Identification of probable pectinesterase as a major allergen of pollen of the Asian white birch (Betula platyphylla) in northern China
JF  - Asian Pac J Allergy Immunol
KW  - birch pollen allergy
KW  - betula platyphylla
KW  - chinese population
KW  - major allergen
KW  - pectinesterase
Y1  - 
U6  - http://dx.doi.org/10.12932/AP-100722-1409
ER  - 
TY  - GEN
A1  - Eidi, Nada
A1  - Balz, Andrea
A1  - Forjan, Mathias
T1  - MedTech-mR - Creating a Virtual Enviroment for Medical Training and Room Planning
T2  - Abstracts of the 2022 Joint Annual Conference of the Austrian (ÖGBMT), German (VDE DGBMT) and Swiss (SSBE) Societies for Biomedical Engineering, including the 14th Vienna International Workshop on Functional Electrical Stimulation
KW  - Medical Training
KW  - Virtual Environment
Y1  - 
U6  - http://dx.doi.org/https://doi.org/10.1515/bmt-2022-2001
SP  - 349
ER  - 
TY  - GEN
A1  - Malaskova, Michaela
A1  - Forjan, Mathias
T1  - Current topics in Life Science Engineering lectures
T2  - Abstracts of the 2022 Joint Annual Conference of the Austrian (ÖGBMT), German (VDE DGBMT) and Swiss (SSBE) Societies for Biomedical Engineering, including the 14th Vienna International Workshop on Functional Electrical Stimulation
KW  - Life Science Engineering
KW  - Teaching
Y1  - 
U6  - http://dx.doi.org/https://doi.org/10.1515/bmt-2022-2001
SP  - 350
ER  - 
TY  - JOUR
A1  - Romanelli, Pasquale
A1  - Bieler, Lara
A1  - Heimel, Patrick
A1  - Škokić, Siniša
A1  - Jakubecova, Dominika
A1  - Kreutzer, Christina
A1  - Zaunmair, Pia
A1  - Smolčić, Tomislav
A1  - Benedetti, Bruno
A1  - Rohde, Eva
A1  - Gimona, Mario
A1  - Hercher, David
A1  - Dobrivojević Radmilović, Marina
A1  - Couillard-Despres, Sebastien
T1  - Enhancing Functional Recovery Through Intralesional Application of Extracellular Vesicles in a Rat Model of Traumatic Spinal Cord Injury
JF  - Front Cell Neurosci
N2  - Local inflammation plays a pivotal role in the process of secondary damage after spinal cord injury. We recently reported that acute intravenous application of extracellular vesicles (EVs) secreted by human umbilical cord mesenchymal stromal cells dampens the induction of inflammatory processes following traumatic spinal cord injury. However, systemic application of EVs is associated with delayed delivery to the site of injury and the necessity for high doses to reach therapeutic levels locally. To resolve these two constraints, we injected EVs directly at the lesion site acutely after spinal cord injury. We report here that intralesional application of EVs resulted in a more robust improvement of motor recovery, assessed with the BBB score and sub-score, as compared to the intravenous delivery. Moreover, the intralesional application was more potent in reducing inflammation and scarring after spinal cord injury than intravenous administration. Hence, the development of EV-based therapy for spinal cord injury should aim at an early application of vesicles close to the lesion.
KW  - exosomes
KW  - inflammation
KW  - locomotion
KW  - neuroimaging
KW  - motor function
Y1  - 
U6  - http://dx.doi.org/10.3389/fncel.2021.795008
VL  - 15
ER  - 
TY  - JOUR
A1  - Feichtinger, Xaver
A1  - Heimel, Patrick
A1  - Tangl, Stefan
A1  - Keibl, Claudia
A1  - Nürnberger, Sylvia
A1  - Schanda, Jakob Emanuel
A1  - Hercher, David
A1  - Kocijan, Roland
A1  - Redl, Heinz
A1  - Grillari, Johannes
A1  - Fialka, Christian
A1  - Mittermayr, Rainer
T1  - Improved biomechanics in experimental chronic rotator cuff repair after shockwaves is not reflected by bone microarchitecture
JF  - PLoS One
KW  - chronic rotator cuff repair
KW  - bone microarchitecture
Y1  - 
U6  - http://dx.doi.org/10.1371/journal.pone.0262294
VL  - 17
IS  - 1
ER  - 
TY  - THES
A1  - Tomasch, Janine
T1  - Strategies to improve the myogenic outcome of skeletal muscle tissue engineering approaches through optimization of biomaterial properties and mechanical stimuli
KW  - muscle
KW  - bioreactor
KW  - tissue engineering
KW  - fibrin
KW  - biomaterial
Y1  - 
ER  - 
TY  - GEN
A1  - Langer, Sarah
A1  - Nemec, Iris
A1  - Kollmitzer, Josef
A1  - Scherer, Matthias
A1  - Martinek, Johannes
T1  - Vergleich des decompositionierten und des überlagerten Elektromyogramms zur Analyse der Muskelermüdung
N2  - Ziel dieser Studie war das überlagerte Elektromyogramm (ueEMG) und das decompositionierte (dEMG) auf deren Reliabilität und Aussagekraft zu überprüfen. Hierfür vollzogen zehn gesunde männliche Probanden zu drei Testzeitpunkten isometrische Ermüdungsmessungen. Hintergrund Bei der Messung des ueEMG werden die Motor Units Aktionspotentiale (MUAPS) elektrisch überlagert. Physiologische Muskelermüdung zeigt sich hier unter anderem in der Veränderung des Frequenzspektrums und der Signalamplitude. Letzteres steigt bei Ermüdung an, wohingegen die mediane Frequenz (MF) sinkt. Eine weitere Möglichkeit die Muskelermüdung mittels Oberflächen-EMG zu ermitteln ist die Anwendung des dEMGs. Dieses ermöglicht mithilfe einer Mustererkennungssoftware einzelne MUAPS zu erkennen. Studien zeigten, dass aktive Motor Units (MU) bei wiederholten oder anhaltenden submaximalen Kontraktionen als Muskelermüdung ihre Feuerungsrate erhöhen und neue MU rekrutiert werden. Bisher wurden diese Methoden jeweils einzeln betrachtet. Um sicherzustellen welcher Ansatz für klinische Daten geeigneter ist, wurden Datensätze zu beiden Methoden gleichzeitig generiert und hinsichtlich ihrer Aussagekraft und Wiederholbarkeit mit statistischen Mitteln verglichen.
KW  - Elektromyogramm
KW  - Rehabilitationstechnik
Y1  - 2022
ER  -