TY  - JOUR
A1  - Holnthoner, Wolfgang
T1  - Adipose-tissue-derived therapeutic cells in their natural environment as an autologous cell therapy strategy: the microtissue-stromal vascular fraction
JF  - Eur Cell Mater.
N2  - The prerequisite for a successful clinical use of autologous adipose-tissue-derived cells is the highest possible regenerative potential of the applied cell population, the stromal vascular fraction (SVF). Current isolation methods depend on high enzyme concentration, lysis buffer, long incubation steps and mechanical stress, resulting in single cell dissociation. The aim of the study was to limit cell manipulation and obtain a derivative comprising therapeutic cells (microtissue-SVF) without dissociation from their natural extracellular matrix, by employing a gentle good manufacturing practice (GMP)-grade isolation. The microtissue-SVF yielded larger numbers of viable cells as compared to the improved standard-SVF, both with low enzyme concentration and minimal dead cell content. It comprised stromal tissue compounds (collagen, glycosaminoglycans, fibroblasts), capillaries and vessel structures (CD31+, smooth muscle actin+). A broad range of cell types was identified by surface-marker characterisation, including mesenchymal, haematopoietic, pericytic, blood and lymphatic vascular and epithelial cells. Subpopulations such as supra-adventitial adipose-derived stromal/stem cells and endothelial progenitor cells were significantly more abundant in the microtissue-SVF, corroborated by significantly higher potency for angiogenic tube-like structure formation in vitro. The microtissue-SVF showed the characteristic phenotype and tri-lineage mesenchymal differentiation potential in vitro and an immunomodulatory and pro-angiogenic secretome. In vivo implantation of the microtissue-SVF combined with fat demonstrated successful graft integration in nude mice. The present study demonstrated a fast and gentle isolation by minor manipulation of liposuction material, achieving a therapeutically relevant cell population with high vascularisation potential and immunomodulatory properties still embedded in a fraction of its original matrix.
KW  - Adipose-tissue
KW  - autologous cell therapy
KW  - microtissue
KW  - stromal vascular fraction
KW  - vascularization
Y1  - 2020
VL  - 2019
IS  - Feb, 37
SP  - 113
EP  - 133
ER  - 
TY  - JOUR
A1  - Sattinger, Vinzenz
A1  - Papa, Maximilian
A1  - Stuja, Kemajl
A1  - Kubinger, Wilfried
T1  - Methodik zur Entwicklung sicherer kollaborativer Produktionssysteme im Rahmen von Industrie 4.0
JF  - e & i Elektrotechnik und Informationstechnik
KW  - Robotics
KW  - Industry 4.0
Y1  - 
ER  - 
TY  - JOUR
A1  - Heimel, Patrick
A1  - Swiadek, Nicole V.
A1  - Slezak, Paul
A1  - Kerbl, Markus
A1  - Schneider, Cornelia
A1  - Nürnberger, Sylvia
A1  - Redl, Heinz
A1  - Teuschl, Andreas
A1  - Hercher, David
T1  - Iodine-Enhanced Micro-CT Imaging of Soft Tissue on the Example of Peripheral Nerve Regeneration
JF  - Contrast Media & Molecular Imaging
KW  - µCT
KW  - Imaging
KW  - Tissue Engineering
KW  - Tissue Regeneration
Y1  - 
ER  - 
TY  - CHAP
A1  - Himmelstoß, Felix
A1  - Edelmoser, Karl
T1  - Zero-Current-Switching Boost Converter, Power Conversion and Intelligent Motion
T2  - Proceedings of PCIM 2019, Nuernberg, Germany, May 7-9, 2019
KW  - Discontinuous Conduction Mode
KW  - DC/DC Converter
KW  - Resonant Converter
KW  - ZCS
KW  - DCM
Y1  - 2019
SN  - 978-3-8007-4938-6
SP  - 1686
EP  - 1691
ER  - 
TY  - CHAP
A1  - Himmelstoß, Felix
A1  - Votzi, Helmut
T1  - A family of quadratic DC/DC converters with one low-side switch and a tapped inductor at the output side
T2  - Aegean Conference on Electrical Machines and Power Electronics & Optimization of Electrical & Electronic Equipment Conference,  Istanbul Turkey, August, 27 – 29, 2019
KW  - DC/DC Converter
KW  - Tapped Inductor
Y1  - 2019
ER  -