Cyclic Tensile Stress Induces Skeletal Muscle Hypertrophy and Myonuclear Accretion in a 3D Model

  • Skeletal muscle is highly adaptive to mechanical stress due to its resident stem cells and the pronounced level of myotube plasticity. Herein, we study the adaptation to mechanical stress and its underlying molecular mechanisms in a tissue-engineered skeletal muscle model. We subjected differentiated 3D skeletal muscle-like constructs to cyclic tensile stress using a custom-made bioreactor system, which resulted in immediate activation of stress-related signal transducers (Erk1/2, p38). Cell cycle re-entry, increased proliferation, and onset of myogenesis indicated subsequent myoblast activation. Furthermore, elevated focal adhesion kinase and β-catenin activity in mechanically stressed constructs suggested increased cell adhesion and migration. After 3 days of mechanical stress, gene expression of the fusogenic markers MyoMaker and MyoMixer, myotube diameter, myonuclear accretion, as well as S6 activation, were significantly increased. Our results highlight that we established a promising tool to study sustained adaptation to mechanical stress in healthy, hypertrophic, or regenerating skeletal muscle.

Export metadata

Additional Services

Share in Twitter Search Google Scholar
Metadaten
Author:Janine Tomasch, Babette Maleiner, Carina HromadaORCiD, Dorota Szwarc-Hofbauer, Andreas Teuschl-Woller
Parent Title (English):Tissue Eng. Part A.
Document Type:Article
Language:English
Completed Date:2023/03/01
Responsibility for metadata:Fachhochschule Technikum Wien
Release Date:2023/06/28
GND Keyword:fibrin; hypertrophy; regeneration; tensile stress; tissue engineering
Volume:2023
Issue:Mar
First Page:257
Last Page:268
Publish on Website:1
Open Access:0
Reviewed:1
Department:Department Life Science Engineering
Research Focus:Tissue Engineering & Molecular Life Science Technologies
Projects:FWF
Studienjahr:2022/2023