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A Turbulent Context
(2023)
Interface with WP123200
(2023)
Regeneration of bone defects is often limited due to compromised bone tissue physiology. Previous studies suggest that engineered extracellular matrices enhance the regenerative capacity of mesenchymal stromal cells. In this study, we used human-induced pluripotent stem cells, a scalable source of young mesenchymal progenitors (hiPSC-MPs), to generate extracellular matrix (iECM) and test its effects on the osteogenic capacity of human bone-marrow mesenchymal stromal cells (BMSCs). iECM was deposited as a layer on cell culture dishes and into three-dimensional (3D) silk-based spongy scaffolds. After decellularization, iECM maintained inherent structural proteins including collagens, fibronectin and laminin, and contained minimal residual DNA. Young adult and aged BMSCs cultured on the iECM layer in osteogenic medium exhibited a significant increase in proliferation, osteogenic marker expression, and mineralization as compared to tissue culture plastic. With BMSCs from aged donors, matrix mineralization was only detected when cultured on iECM, but not on tissue culture plastic. When cultured in 3D iECM/silk scaffolds, BMSCs exhibited significantly increased osteogenic gene expression levels and bone matrix deposition. iECM layer showed a similar enhancement of aged BMSC proliferation, osteogenic gene expression, and mineralization compared with extracellular matrix layers derived from young adult or aged BMSCs. However, iECM increased osteogenic differentiation and decreased adipocyte formation compared with single protein substrates including collagen and fibronectin. Together, our data suggest that the microenvironment comprised of iECM can enhance the osteogenic activity of BMSCs, providing a bioactive and scalable biomaterial strategy for enhancing bone regeneration in patients with delayed or failed bone healing.
Technologiemanagement
(2023)
Stellhebel beim Aufbau von Corporate Start-ups. Dargestellt am Start-Up-Projekt „Hallo Sonne“
(2023)
Bucket increasing trees are multilabelled generalizations of increasing trees, where each non-leaf node carries b labels, with a fixed integer. We provide a fundamental result, giving a complete characterization of all families of bucket increasing trees that can be generated by a tree evolution process. We also provide several equivalent properties, complementing and extending earlier results for ordinary increasing trees to bucket trees. Additionally, we state second order results for the number of descendants of label j, again extending earlier results in the literature.
Dieser Beitrag soll interessierten Laien eine Hilfestellung an die Hand geben, ihre Infomaterialien barriereärmer zu gestalten. Einführend erfolgt eine kurze Darstellung der rechtlichen und gesellschaftlichen Situation rund um das Thema Behinderung. Verschiedene Ebenen von Barrieren und Lösungsansätze werden vorgestellt: Organisationale Barrieren von Bildungseinrichtungen werden ebenso wie Barrieren rund um das Bildungsberatungsgespräch adressiert. Der Schwerpunkt liegt bei auf der Gestaltung von Informationsmaterial. Neben einem theoretischen Hintergrund über Lösungsansätze werden praktische Umsetzungen für verschiedene Medien vorgestellt. Der Beitrag wird durch Beispiele guter Praxis in der Umsetzung, eine Checkliste zur Anwendung und weiterführende Ressourcen abgerundet.