Sonstiges
Refine
Year of publication
Document Type
- Conference Proceeding (50)
- Lecture (45)
- Article (35)
- Part of a Book (6)
- Book (3)
- Doctoral Thesis (1)
- Report (1)
Keywords
- Sport (38)
- Electrical Stimulation (21)
- Muscle Stimulation (18)
- Biomedical Engineering (10)
- Muscle Denervation (6)
- Sports Equipment Technologies (6)
- Teaching (6)
- Cycling (5)
- Golf (4)
- Running (4)
- Skiing (4)
- Accelerometer Data (3)
- Machine Learning (3)
- Nordic Walking (3)
- Prevention (3)
- Road Surface (3)
- Running Shoes (3)
- Biomechanics (2)
- Biosignals (2)
- Breathing Simulation (2)
- Climbing (2)
- Coordination (2)
- Didactics (2)
- Electric Stimulation (2)
- Handcycling (2)
- Motivation (2)
- Motocross (2)
- Multiphysics (2)
- Muscle Activity (2)
- Muscular Activity (2)
- Physiology (2)
- Stand-Up Paddling (2)
- extracellular matrix (2)
- mechanical lung-simulator (2)
- 3D Anthropometry (1)
- Acceleration Sensors (1)
- Amplitude (1)
- Amputation (1)
- Arch ratio (1)
- Archery (1)
- Back Training (1)
- Baseball (1)
- Body Climate (1)
- Brake (1)
- CEN (1)
- COVID19 (1)
- Cancer (1)
- Compatibility (1)
- Computer Science (1)
- Contact pressure (1)
- Customized Equipment (1)
- Data Analysis (1)
- Data Collection (1)
- Denervation (1)
- Diabetes (1)
- Dry powder inhaler resistance (1)
- Electroneurodiagnostics (1)
- Elektromyogramm (1)
- Engineering (1)
- Footwear (1)
- Footwear design (1)
- Friction (1)
- Genetics (1)
- Health data (1)
- Healthcare (1)
- Hiking (1)
- Human Cells (1)
- Ice Hockey (1)
- Implants (1)
- Internationalized Teaching (1)
- Intraocular Lenses (1)
- Laboratory (1)
- Life Science Engineering (1)
- Lung Simulation (1)
- Lung Simulator (1)
- Lungensimulation (1)
- Magnetic (1)
- Materials (1)
- Measuring (1)
- Medical Training (1)
- Montainbiking (1)
- Motion Analysis (1)
- Muscle Excitation (1)
- Muscle Fiber (1)
- Neck Brace (1)
- Neurons (1)
- Paralympics (1)
- Problem Based Learning (1)
- Questionnaires (1)
- Rehabilitationstechnik (1)
- Rehabitation (1)
- Rowing (1)
- SARS-COV2 (1)
- Security (1)
- Sensor (1)
- Shoes (1)
- Ski Touring (1)
- Snowboard (1)
- Soccer (1)
- Stimulator (1)
- Sustainability (1)
- TR-MS (1)
- Telemonitoring (1)
- Tennis (1)
- Test Bench (1)
- Testing (1)
- Thigh (1)
- Toll-like receptor 3 (1)
- Treadmill (1)
- Tumor (1)
- Underwear (1)
- Virtual Environment (1)
- Voles (1)
- Wheels (1)
- aging (1)
- aortic valve disease (1)
- benchmark (1)
- betula platyphylla (1)
- biglycan (1)
- biomaterial (1)
- bioreactor (1)
- birch pollen allergy (1)
- bone marrow stromal cells (1)
- bone microarchitecture (1)
- bone tissue engineering (1)
- breathomics (1)
- chinese population (1)
- chronic rotator cuff repair (1)
- eHealth (1)
- education (1)
- endochondral (1)
- exosomes (1)
- extra-curricular offerings (1)
- fibrin (1)
- iPSCs (1)
- inflammation (1)
- inspiratory flow rate (1)
- intercultural teaching (1)
- intramembranous (1)
- locomotion (1)
- lung simulation (1)
- major allergen (1)
- mechanical upper airway model (1)
- mesenchymal stromal cells (1)
- motor function (1)
- muscle (1)
- neuroimaging (1)
- optical aerosol spectrometry (1)
- ossification (1)
- osteogenesis (1)
- osteogenic differentiation (1)
- pectinesterase (1)
- peppermint (1)
- standardization (1)
- tissue engineering (1)
Department
- Department Life Science Engineering (141) (remove)
Local inflammation plays a pivotal role in the process of secondary damage after spinal cord injury. We recently reported that acute intravenous application of extracellular vesicles (EVs) secreted by human umbilical cord mesenchymal stromal cells dampens the induction of inflammatory processes following traumatic spinal cord injury. However, systemic application of EVs is associated with delayed delivery to the site of injury and the necessity for high doses to reach therapeutic levels locally. To resolve these two constraints, we injected EVs directly at the lesion site acutely after spinal cord injury. We report here that intralesional application of EVs resulted in a more robust improvement of motor recovery, assessed with the BBB score and sub-score, as compared to the intravenous delivery. Moreover, the intralesional application was more potent in reducing inflammation and scarring after spinal cord injury than intravenous administration. Hence, the development of EV-based therapy for spinal cord injury should aim at an early application of vesicles close to the lesion.
Bone grafts can be engineered by differentiating human mesenchymal stromal cells (MSCs) via the endochondral and intramembranous ossification pathways. We evaluated the effects of each pathway on the properties of engineered bone grafts and their capacity to drive bone regeneration. Bone-marrow-derived MSCs were differentiated on silk scaffolds into either hypertrophic chondrocytes (hyper) or osteoblasts (osteo) over 5 weeks of in vitro cultivation, and were implanted subcutaneously for 12 weeks. The pathways' constructs were evaluated over time with respect to gene expression, composition, histomorphology, microstructure, vascularization and biomechanics. Hypertrophic chondrocytes expressed higher levels of osteogenic genes and deposited significantly more bone mineral and proteins than the osteoblasts. Before implantation, the mineral in the hyper group was less mature than that in the osteo group. Following 12 weeks of implantation, the hyper group had increased mineral density but a similar overall mineral composition compared with the osteo group. The hyper group also displayed significantly more blood vessel infiltration than the osteo group. Both groups contained M2 macrophages, indicating bone regeneration. These data suggest that, similar to the body's repair processes, endochondral pathway might be more advantageous when regenerating large defects, whereas intramembranous ossification could be utilized to guide the tissue formation pattern with a scaffold architecture.
Regeneration of bone defects is often limited due to compromised bone tissue physiology. Previous studies suggest that engineered extracellular matrices enhance the regenerative capacity of mesenchymal stromal cells. In this study, we used human-induced pluripotent stem cells, a scalable source of young mesenchymal progenitors (hiPSC-MPs), to generate extracellular matrix (iECM) and test its effects on the osteogenic capacity of human bone-marrow mesenchymal stromal cells (BMSCs). iECM was deposited as a layer on cell culture dishes and into three-dimensional (3D) silk-based spongy scaffolds. After decellularization, iECM maintained inherent structural proteins including collagens, fibronectin and laminin, and contained minimal residual DNA. Young adult and aged BMSCs cultured on the iECM layer in osteogenic medium exhibited a significant increase in proliferation, osteogenic marker expression, and mineralization as compared to tissue culture plastic. With BMSCs from aged donors, matrix mineralization was only detected when cultured on iECM, but not on tissue culture plastic. When cultured in 3D iECM/silk scaffolds, BMSCs exhibited significantly increased osteogenic gene expression levels and bone matrix deposition. iECM layer showed a similar enhancement of aged BMSC proliferation, osteogenic gene expression, and mineralization compared with extracellular matrix layers derived from young adult or aged BMSCs. However, iECM increased osteogenic differentiation and decreased adipocyte formation compared with single protein substrates including collagen and fibronectin. Together, our data suggest that the microenvironment comprised of iECM can enhance the osteogenic activity of BMSCs, providing a bioactive and scalable biomaterial strategy for enhancing bone regeneration in patients with delayed or failed bone healing.
A major challenge for breath research is the lack of standardization in sampling and analysis. To address this, a test that utilizes a standardized intervention and a defined study protocol has been proposed to explore disparities in breath research across different analytical platforms and to provide benchmark values for comparison. Specifically, the Peppermint Experiment involves the targeted analysis in exhaled breath of volatile constituents of peppermint oil after ingestion of the encapsulated oil. Data from the Peppermint Experiment performed by proton transfer reaction mass spectrometry (PTR-MS) and selected ion flow tube mass spectrometry (SIFT-MS) are presented and discussed herein, including the product ions associated with the key peppermint volatiles, namely limonene, α- and β-pinene, 1,8-cineole, menthol, menthone and menthofuran. The breath washout profiles of these compounds from 65 individuals were collected, comprising datasets from five PTR-MS and two SIFT-MS instruments. The washout profiles of these volatiles were evaluated by comparing the log-fold change over time of the product ion intensities associated with each volatile. Benchmark values were calculated from the lower 95% confidence interval of the linear time-to-washout regression analysis for all datasets combined. Benchmark washout values from PTR-MS analysis were 353 min for the sum of monoterpenes and 1,8-cineole (identical product ions), 173 min for menthol, 330 min for menthofuran, and 218 min for menthone; from SIFT-MS analysis values were 228 min for the sum of monoterpenes, 281 min for the sum of monoterpenes and 1,8-cineole, and 370 min for menthone plus 1,8-cineole. Large inter- and intra-dataset variations were observed, whereby the latter suggests that biological variability plays a key role in how the compounds are absorbed, metabolized and excreted from the body via breath. This variability seems large compared to the influence of sampling and analytical procedures, but further investigations are recommended to clarify the effects of these factors.
Dry powder inhalers are used by a large number of patients worldwide to treat respiratory diseases. The objective of this work is to experimentally investigate changes in aerosol particle diameter and particle number concentration of pharmaceutical aerosols generated by four dry powder inhalers under realistic inhalation and exhalation conditions. To simulate patients undergoing inhalation therapy, the active respiratory system model (xPULM™) was used. A mechanical upper airway model was developed, manufactured, and introduced as a part of the xPULM™ to represent the human upper respiratory tract with high fidelity. Integration of optical aerosol spectrometry technique into the setup allowed for evaluation of pharmaceutical aerosols. The results show that there is a significant difference (p < 0.05) in mean particle diameter between inhaled and exhaled particles with the majority of the particles depositing in the lung, while particles with the size of (>0.5 μm) are least influenced by deposition mechanisms. The fraction of exhaled particles ranges from 2.13% (HandiHaler®) over 2.94% (BreezHaler®), and 6.22% (Turbohaler®) to 10.24% (Ellipta®). These values are comparable to previously published studies. Furthermore, the mechanical upper airway model increases the resistance of the overall system and acts as a filter for larger particles (>3 μm). In conclusion, the xPULM™ active respiratory system model is a viable option for studying interactions of pharmaceutical aerosols and the respiratory tract regarding applicable deposition mechanisms. The model strives to support the reduction of animal experimentation in aerosol research and provides an alternative to experiments with human subjects.
Ziel dieser Studie war das überlagerte Elektromyogramm (ueEMG) und das decompositionierte (dEMG) auf deren Reliabilität und Aussagekraft zu überprüfen. Hierfür vollzogen zehn gesunde männliche Probanden zu drei Testzeitpunkten isometrische Ermüdungsmessungen. Hintergrund Bei der Messung des ueEMG werden die Motor Units Aktionspotentiale (MUAPS) elektrisch überlagert. Physiologische Muskelermüdung zeigt sich hier unter anderem in der Veränderung des Frequenzspektrums und der Signalamplitude. Letzteres steigt bei Ermüdung an, wohingegen die mediane Frequenz (MF) sinkt. Eine weitere Möglichkeit die Muskelermüdung mittels Oberflächen-EMG zu ermitteln ist die Anwendung des dEMGs. Dieses ermöglicht mithilfe einer Mustererkennungssoftware einzelne MUAPS zu erkennen. Studien zeigten, dass aktive Motor Units (MU) bei wiederholten oder anhaltenden submaximalen Kontraktionen als Muskelermüdung ihre Feuerungsrate erhöhen und neue MU rekrutiert werden. Bisher wurden diese Methoden jeweils einzeln betrachtet. Um sicherzustellen welcher Ansatz für klinische Daten geeigneter ist, wurden Datensätze zu beiden Methoden gleichzeitig generiert und hinsichtlich ihrer Aussagekraft und Wiederholbarkeit mit statistischen Mitteln verglichen.